编码DNA复制蛋白和病理相关蛋白的基因 【发明背景】
细胞中天然进行的代谢过程所产生的特定产物和副产物可以用于工业的许多分支中，包括食品工业、动物饲料工业、化妆品工业及制药工业。这些分子合起来称为“精细化学品”，其包括有机酸、蛋白原性(protainogenic)和非蛋白原性氨基酸、核苷酸和核苷、脂类和脂肪酸、二醇、碳水化合物、芳香化合物、维生素和辅因子、以及酶。它们的最好的产生途径是大规模培养细菌，针对不同的特定情况，这些细菌被开发出来以产生并分泌大量的期望分子。一种对此目的尤其适用的生物体是谷氨酸棒状杆菌(Corynebacterium glutamicum)，其为革兰氏阳性的非致病细菌。通过菌株选择，已开发了许多突变菌株，它们能产生各种期望化合物。但是，对具有提高的特定分子产量的菌株的筛选是一种费时且困难的方法。
发明简述
本发明提供了新的可用于谷氨酸棒状杆菌或相关菌种的鉴定或分类的核酸分子。谷氨酸棒状杆菌为革兰氏阳性需氧细菌，其广泛地用在工业上用于大规模产生多种精细化学品，也用于降解碳氢化合物(如在原油泄漏物中)，以及用于氧化萜类化合物。本发明的核酸分子因此可用于鉴定能如通过发酵法用来产生精细化学品的微生物。虽然谷氨酸棒状杆菌自身为非致病的，但它与作为人类地主要病原体的其它谷氨酸棒状杆菌菌种，如白喉棒状杆菌(Corynebacterium diphtheriae)(白喉的致病因子)有亲缘关系。所以能够鉴定棒状杆菌的存在也具有显著的临床重要性，例如诊断应用。而且，所述核酸分子可作为谷氨酸棒状杆菌或相关生物基因组作图的参考点。
这些新核酸分子编码的蛋白质文中称为DNA复制、核糖体的和病理(RRP)蛋白。这些RRP蛋白例如可直接或间接参与谷氨酸棒状杆菌中一种或多种精细化学品的生产。本发明的RRP蛋白也可参与降解碳氢化合物或氧化萜类化合物。这些蛋白能用于鉴定谷氨酸棒状杆菌或谷氨酸棒状杆菌相关生物；在一个蛋白混合物中谷氨酸棒状杆菌或相关种的特异性RRP蛋白的存在可以指示样品中所述细菌的存在。此外，这些RRP-蛋白在植物或动物中可能具有与病害状态或疾病有关的同系物；因此，这些蛋白可作为药物筛选和开发治疗化性合物的有用的药学靶标。
由于可得到用于谷氨酸棒状杆菌中的克隆载体，例如在Sinskey等，美国专利No.4,649,119中所公开的那些，和用于遗传操作谷氨酸棒状杆菌和相关短杆菌种(如乳糖发酵短杆菌(lactofermentum))的技术(Yoshihama等，细菌学杂志(J.Bacteriol.)162：591-597(1985)；Katsumata等，细菌学杂志(J.Bacteriol.)159：306-311(1984)；和Santamaria等，普通微生物学杂志(J.Gen.Microbiol.)130：2237-2246(1984))，本发明的核酸分子可用于遗传操作所述的生物体，以调节一种或多种精细化学品的产生。这种调节可由于操作本发明的基因的直接效果，或可由于此类操作的间接效果而发生。例如，有可能通过修饰参与精细化学品生物合成或者降解的蛋白的活性(即，通过相应基因的突变)，直接调节细胞合成或降解所述化合物的能力，由此调节精细化学品的产率和/或生产效率。同样地，有可能通过调节调控精细化学品代谢途径的蛋白的活性直接影响期望化合物的产量，无论产量是上调还是下调，两种方式都将调节来自细胞的精细化学品的产率或生产效率。
也可以通过修饰本发明的蛋白的活性(即通过相应基因的突变)，使细胞生长或分裂或维持存活和生产力的能力总体上得以提高而进行精细化学品产生的间接调节。精细化学品通常通过在经常是对生长或细胞分裂次最佳的条件下大规模发酵培养谷氨酸棒状杆菌来生产。修饰本发明的蛋白(如应激反应蛋白、细胞壁蛋白或者与细胞分裂和细胞生长发生所必需的化合物如核苷酸和氨基酸的代谢有关的蛋白)以至于所述条件下细胞更好地生存、生长和繁殖，将能够使大规模培养中所述修饰的谷氨酸棒状杆菌细胞的数量和生产力提高，并且这接下来将提高一种或多种期望精细化学品的产率和/或生产效率。此外，细胞代谢途径必然地彼此相依并被共同调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性)，可同时改变这个微生物中另一代谢途径的活性或调节，而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
存在许多机制，通过这些机制本发明RRP蛋白的修饰可影响含有该修饰的RRP蛋白的谷氨酸棒状杆菌菌株中精细化学品的产率、产量或生产力。例如，通过加快DNA复制速率(如通过优化一个或多个DNA聚合酶的活性，或通过提高拓扑异构酶或解旋酶解旋DNA的速率)可以加快细胞分裂，而这接下来可增加培养物中生产精细化学品的活细胞的数量。类似地，通过提高翻译速率(如通过优化核糖体蛋白的活性)可以提高细胞中参与合成一种或多种目的精细化学品的蛋白的数量。当培养具有适当修饰的RRP蛋白的微生物时，这两种方法都应导致目的精细化学品产量的提高。
修饰本发明的DNA复制蛋白也可导致更大的复制准确性，并因此提高所述微生物的遗传稳定性和生存力；由此降低另一遗传修饰由于错误复制而被破坏的危险性，而所述遗传修饰可提高所述精细化学品的产量。
本发明提供了编码这里称作为RRP蛋白的蛋白的新核酸分子，所述RRP蛋白能够，例如调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率，或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。编码RRP蛋白的核酸分子这里称作为RRP核酸分子。在优选的实施方案中，RRP蛋白能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或产率，或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。此类蛋白的实例是表1中所列基因编码的那些蛋白。
因此，本发明的一个方面涉及含有编码RRP蛋白或其生物学活性部分的核苷酸序列的分离的核酸分子(例如cDNA)和适合作为检测或扩增RRP编码核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在尤其优选的实施方案中，该分离的核酸分子含有集合A所列出的任一核酸序列或这些核酸序列之任一的编码区或其互补序列。在其他优选的实施方案中，该分离的核酸分子编码集合B中列出的氨基酸序列之一。本发明优选的RRP蛋白还优选具有至少一种本文所描述的RRP活性。
以下将序列表中的核酸序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合A。
以下将序列表中的多肽序列以及下表1中描述的在相关位置处的序列修饰一起定义为集合B。
在另一个实施方案中，分离的核酸分子长度至少15个核苷酸并且在严紧条件下与含有集合A的核苷酸序列的核酸分子杂交。分离的核酸分子优选与天然发生的核酸分子对应。分离的核酸更优选编码天然发生的谷氨酸棒状杆菌RRP蛋白或其具生物活性的部分。
本发明的另一个方面涉及载体(例如重组表达载体)，其含有本发明的核酸分子，还涉及已导入所述载体的宿主细胞。在一个实施方案中，通过使用在合适的培养基中培养的宿主细胞制备RRP蛋白。可以从培养基或宿主细胞中分离该RRP蛋白。
本发明的另一个方面涉及其中导入了RRP基因或其RRP基因被修饰的遗传修饰的微生物。在一个实施方案中，通过导入至少一种编码突变RRP序列的本发明核酸分子作为转基因，修饰了所述微生物的基因组。在另一个实施方案中，通过与修饰的RRP基因的同源重组，修饰(例如破坏功能)了所述微生物基因组中的内源性RRP基因。在一个优选的实施方案中，微生物属于棒状杆菌或短杆菌(Brevibacterium)属，尤其优选谷氨酸棒状杆菌。在一个优选的实施方案中，该微生物也被用于制备目的化合物，如氨基酸、尤其优选赖氨酸。
另一个优选的实施方案是具有一种以上的集合A中描述的核酸分子的宿主细胞。可用各种技术人员已知的方法制备这些宿主细胞。例如，可通过携带几种本发明核酸分子的载体转染这些细胞。然而，也可以使用载体每一种情况下向宿主细胞中导入一种本发明的核酸分子并从而同时或相继使用多个载体。从而可能构建携带许多乃至高达数百的本发明核酸序列的宿主细胞。这种积累常能够对宿主细胞的精细化学品的生产力产生超加性效果。
本发明的另一个方面涉及分离的RRP蛋白或其部分，例如具有生物学活性的部分。在优选的实施方案中，该分离的RRP蛋白或其部分能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率，或者能够作为谷氨酸棒状杆菌或相关生物体的鉴定标记。在另一个优选的实施方案中，该分离的RRP蛋白或其部分与集合B的氨基酸序列充分同源，以至该蛋白或其部分仍然能够例如调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率，或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
此外，本发明涉及分离的RRP蛋白制品。在优选的实施方案中，该RRP蛋白含有集合B的氨基酸序列。在另一个优选的实施方案中，本发明涉及分离的完整长度的蛋白，其和集合B的完整氨基酸序列(其由集合A中的可读框编码)基本同源。
可以将RRP多肽或其生物学活性部分与非RRP多肽功能性地连接以产生融合蛋白。在优选的实施方案中，该融合蛋白和单独的RRP蛋白具有不同的活性，在其它优选的实施方案中，所述融合蛋白能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率，或者能够作为谷氨酸棒状杆菌或相关生物体的鉴定标记。在尤其优选的实施方案中，所述融合蛋白整合在宿主细胞中调节细胞对目的化合物的生产。
本发明的另一个方面涉及制备精细化学品的制备方法。该方法涉及含有可导致本发明RRP核酸分子表达的载体的细胞的培养，从而生产精细化学品。在优选的实施方案中，该方法还包括得到含有此载体的细胞的步骤，其中所述细胞被导致RRP核酸表达的载体转染。在另一个优选的实施方案中，所述方法还包括从培养物中得到精细化学品的步骤。在一个优选的实施方案中，该细胞属于棒状杆菌属或短杆菌属。
本发明的另一个方面涉及调节微生物生产分子的方法。这些方法包括将细胞和调节RRP蛋白活性或RRP核酸表达的物质接触使得细胞的相关活性和没有所述物质存在时的相同活性相比被改变。在一个优选的实施方案中，针对一个或多个谷氨酸棒状杆菌RRP蛋白活性对细胞进行调节，以提高该微生物生产目的精细化学品的产率、产量和/或生产效率。调节RRP蛋白活性的物质可以是例如刺激RRP蛋白活性或RRP核酸表达的物质。刺激RRP蛋白活性或RRP核酸表达的物质的例子包括小分子、活性RRP蛋白和已经被导入细胞中的编码RRP蛋白的核酸。抑制RRP活性或RRP表达的物质的例子包括小分子和反义RRP核酸分子。
本发明的另一个方面涉及调节细胞中目的化合物的产率、产量和/或生产效率的方法，包括向细胞中导入RRP野生型基因或RRP突变基因，该基因或者保留在分开的质粒上或者整合到宿主细胞的基因组中。向基因组的整合可随机发生或者通过同源重组发生，从而使得天然基因被整合的拷贝所代替，导致细胞中目的化合物的生产被调节。在优选的实施方案中，所述产率增加了。在另一个优选的实施方案中，化学品是精细化学品，其在一个特别优选的实施方案中是氨基酸。在一个特别优选的实施方案中，该氨基酸是L-赖氨酸。
发明详述
本发明提供了RRP核酸和RRP蛋白分子，它们可用于鉴定谷氨酸棒状杆菌或相关生物体，用于绘制谷氨酸棒状杆菌基因组(或密切相关生物体的基因组)图谱或者用于鉴定可用来例如通过发酵工艺产生精细化学品的微生物。由所述核酸编码的蛋白可用来直接或间接地调节谷氨酸棒状杆菌中一种或多种精细化学品的产量或生产效率，可作为谷氨酸棒状杆菌或相关生物体的鉴定标记，可用于氧化萜类化合物，或者用来降解碳氢化合物，或者作为研发治疗性药学化合物的靶标。本发明的诸方面进一步阐述如下。
I.精细化学品
术语“精细化学品”为本领域所认知，其包括已用于多种工业的由生物体产生的分子，其中所述工业例如但不限于制药工业、农业工业和化妆品工业。此类化合物包括有机酸如酒石酸、衣康酸和二氨基庚二酸、蛋白原的和非蛋白原的氨基酸、嘌呤和嘧啶碱基、核苷和核苷酸(如在Rehm等人编辑的生物技术(Biotechnology)第6卷中Kuninaka，A.(1996)核苷酸和相关化合物(Nucleotides and related compounds)，561-612页，VCH：Weinheim及其中所含参考文献中所述)、脂类、饱和和不饱和脂肪酸(如花生四烯酸)、二醇(如丙二醇和丁二醇)、碳水化合物(如透明质酸和海藻糖)、芳香化合物(如芳香胺、香草醛和靛蓝)、维生素和辅因子(参见如Ullmann工业化学百科全书(Ullmann’s Encyclopedia of Industrial Chemistry)，A27卷，“维生素”(“Vitamins”)，443-613页(1996)VCH：Weinheim及其中的参考文献；以及Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings ofthe UNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press，(1995))、酶以及在Gutcho(1983)通过发酵的化学品(Chemicals by Fermentation)，Noyes DataCorporation，ISBN：0818805086和其中的参考文献中所述的所有其它化学品。这些精细化学品中的某些化学品的代谢和用途进一步阐述如下。
A.氨基酸代谢和用途
氨基酸包含所有蛋白质的基本结构单元，由此为所有生物体中正常功能所必需。术语“氨基酸”为本领域所认知。蛋白原性氨基酸有20种，为蛋白质的结构单元，在蛋白质中它们通过肽键连接，非蛋白原性氨基酸(已知成百种非蛋白原的氨基酸)在蛋白质中通常不存在(参见Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry))，A2卷，57-97页，VCH：Weinheim(1985))。氨基酸可为光学D-或L-构型，尽管在天然存在的蛋白质中发现的唯一类型通常为L-氨基酸。已充分说明了20种蛋白原性氨基酸中每一种在原核和真核细胞中的生物合成和降解途径(参见例如，Stryer，L.生物化学(Biochemistry)，第三版，578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)，这样称呼是因为由于它们生物合成的复杂性，通常必须从食物中摄取它们，它们通过简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物能够合成这些氨基酸中的一部分氨基酸，但必需氨基酸必须从食物中摄取，以进行正常的蛋白质合成。
除了它们在蛋白质生物合成中的功能外，这些氨基酸本身还是令人感兴趣的化学品，并且许多已发现在人类食品、动物饲料、化学品、化妆品、农业和药学工业中具有多种应用。赖氨酸不仅对人的营养而且对单胃家畜如禽和猪都是一种重要的氨基酸。谷氨酸盐是最常用的调味添加剂(谷氨酸单钠盐，MSG)，并且还用于食品工业，天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸均用于药学工业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸用于药学工业和化妆品工业。苏氨酸、色氨酸和D/L-甲硫氨酸是广泛使用的动物饲料添加剂。(Rehm等人(编辑)，生物技术(Biotechnology)，第6卷，第14a章中的Leuchtenberger，W.(1996)Amino aids-technicalproduction and use，466-502页，VCH：Weinheim)。还发现这些氨基酸适用作前体，用于合成合成的氨基酸和蛋白质，如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟色氨以及在Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)，A2卷，57-97页，VCH：Weinheim，1985中所述的其它物质。
在可产生这些天然氨基酸的生物体如细菌中已充分描述了它们的生物合成(细菌氨基酸生物合成及其调节的综述，见Umbarger，H.E.(1978)Ann.Rev.Biochem.47：533-606)。谷氨酸通过还原胺化α-酮戊二酸(柠檬酸循环中的中间体)而合成。谷氨酰胺、脯氨酸和精氨酸接下来由谷氨酸产生。丝氨酸的生物合成为三步，由3-磷酸甘油酸(糖酵解中间体)开始，在氧化、氨基转移和水解步骤后产生该氨基酸。半胱氨酸和甘氨酸两者均产生自丝氨酸；具体地，前者通过高半胱氨酸与丝氨酸缩合，后者在丝氨酸转羟甲基酶催化的反应中通过侧链β-碳原子转移至四氢叶酸上合成。苯丙氨酸和酪氨酸在9步的生物合成途径中合成自糖酵解和磷酸戊糖途径的前体赤藓糖4-磷酸和磷酸烯醇丙酮酸，它们的不同仅在预苯酸合成后的最后两步。色氨酸也从这两种起始分子产生，但其合成为11步的途径。酪氨酸也可在苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸均为来自丙酮酸盐(糖酵解的终产物)的生物合成产物。天冬氨酸形成自草酰乙酸(柠檬酸循环的中间体)。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸均通过转化天冬氨酸而形成。异亮氨酸从苏氨酸形成。组氨酸在一个复杂的9步途径中从一种活化糖——5-磷酸核糖-1-焦磷酸形成。
超过蛋白质生物合成所需的量的氨基酸不能被贮藏，并被降解以提供用于细胞主要代谢途径的中间体(综述参见Stryer，L.生物化学(Biochemistry)第三版，21章，“氨基酸降解和尿素循环”495-516页(1988))。虽然细胞可将不想要的氨基酸转化为有用的代谢中间体，但从所需用于合成氨基酸的能量、前体分子和酶来说，氨基酸的产生是昂贵的。因此氨基酸的生物合成受到反馈抑制调节并不让人吃惊，其中特定氨基酸的存在起到减缓或完全终止其自身产生的作用(关于氨基酸生物合成途径中的反馈机制的综述参见Stryer，L.生物化学(Biochemistry)第三版，24章：”氨基酸和血红素的生物合成”575-600页(1988))。因此，特定氨基酸的产出受到细胞中存在的该氨基酸的量的限制。
B.维生素、辅因子和营养成分的代谢和用途
维生素、辅因子和营养成分包含高等动物不能合成且必须摄取的另一类分子，虽然它们易于被其它生物体如细菌合成。这些分子自身为生物活性物质或为在多种代谢途径中作为电子转移分子或中间体的生物活性物质的前体。除了它们的营养价值外，这些化合物作为着色剂、抗氧化剂和催化剂或其它加工辅助剂也具有重要的工业价值(这些化合物的结构、活性和工业应用的概述参见例如，Ullmann工业化学百科全书(Ullmann’sEncyclopedia of Industrial Chemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996)。术语”维生素”为本领域认知，包括生物体行使正常功能所需的营养素，且该生物体自身不能合成它们。维生素组可以包括辅因子和营养性化合物。术语“辅因子”包括进行正常酶促活性所需的非蛋白质性化合物。此类化合物可为有机的或无机的，本发明的辅因子优选有机的。术语“营养成分”包括促进植物和动物尤其是人的健康的食物添加剂。此类分子的实例为维生素、抗氧化剂和某些脂类(如多不饱和脂肪酸)。
已大量描述了这些分子在可产生它们的生物体如细菌中的生物合成(Ullmann工业化学百科全书(Ullmann’s Encyclopedia of IndustrialChemistry)，“维生素”A27卷，443-613页，VCH：Weinheim，1996；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wiley& Sons；Ong，A.S.，Niki，E.和Packer，L.(1995)UNESCO/马来西亚科学和技术协会和亚州自由基研究协会联盟举办的“营养、脂类、健康和疾病“论文集(“Nutrition，Lipids，Health，and Disease”Proceedings of theUNESCO/Confederation of Scientific and Technological Associations inMalaysia，and the Society for Free Radical Research-Asia)，1994年9月1-3日在马来西亚槟榔屿举行，AOCS Press：Champaign，IL X，374S)。
硫胺素(维生素B1)由嘧啶和噻唑部分通过化学偶联产生。核黄素(维生素B2)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素接下来用于合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。合起来称为‘维生素B6’的化合物类(例如，吡哆素、吡哆胺、5’-磷酸吡哆醛和商业上使用的维生素B6盐酸盐)均为共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸盐(泛酸(R)-(+)-N-(2，4-二羟基-3，3-二甲基-1-氧代丁基)-β-丙氨酸)可通过化学合成或发酵产生。泛酸生物合成的最后步骤由ATP-驱动的β-丙氨酸和泛解酸的缩合组成。负责泛解酸和β-丙氨酸转化及泛酸缩合生物合成步骤的酶是已知的。泛酸盐的代谢活性形式为辅酶A，其生物合成由5步酶促步骤进行。泛酸盐、5’-磷酸吡哆醛、半胱氨酸和ATP为辅酶A的前体。这些酶不仅催化泛酸盐的形成，而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(维生素B5原)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。
已详细研究了微生物中生物素从前体分子庚二酰辅酶A的生物合成，并鉴定了几种所涉及的基因。发现许多相应的蛋白质也参与Fe-簇合成，属于蛋白质nifS类。硫辛酸衍生自辛酸，并在能量代谢中作为辅酶，此时其成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸盐为一组这样的物质，其均为叶酸衍生物，而叶酸来自于L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已详细研究了某些微生物中始于鸟苷-5’-三磷酸(GTP)的生物转化的代谢中间体、L-谷氨酸和对氨基苯甲酸的叶酸及其衍生物的生物合成。
类可啉(如钴胺素且尤其是维生素B12)和卟啉属于一组特征在于四吡咯环体系的化学品。维生素B12的生物合成太复杂，目前尚未彻底解释清楚，但许多涉及的酶和底物现已知。烟酸(烟酸盐)和烟酰胺为吡啶衍生物，也称为‘烟酸(niacin)’。烟酸为重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)和它们的还原形式的前体。
这些化合物的工业规模的生产大多数依赖于无细胞的化学合成，虽然这些化学品中的一些如核黄素、维生素B6、泛酸盐和生物素也已通过微生物的大规模培养物产生。只有维生素B12因为其合成的复杂性是仅通过发酵产生的。体外方法需要支出相当大量的材料和时间，并常常代价很高。
C.嘌呤、嘧啶、核苷和核苷酸的代谢和用途
嘌呤和嘧啶代谢基因及它们相应的蛋白质为治疗肿瘤和病毒感染的重要靶标。术语“嘌呤”或“嘧啶”包括构成核酸、辅酶和核苷酸的含氮碱基。术语“核苷酸”包括核酸分子的基本结构单元，其包括含氮碱基、戊糖(对RNA而言，该糖为核糖，对DNA而言，该糖为D-脱氧核糖)和磷酸。术语“核苷”包括作为核苷酸前体的分子，但其不含核苷酸所具有的磷酸单元。通过抑制这些分子的生物合成，或抑制它们形成核酸分子的动员，可抑制RNA和DNA合成；通过以靶向癌细胞的方式抑制这种活性，可抑制肿瘤细胞分裂和复制的能力。
此外，有不形成核酸分子但作为能量贮存体(即AMP)或作为辅酶(即FAD和NAD)的核苷酸。
一些出版物已描述了这些化学品通过影响嘌呤和/或嘧啶代谢在这些医学适应症中的用途(例如Christopherson，R.I.和Lyons，S.D.(1990)”作为化学治疗剂的嘧啶和嘌呤从头生物合成的强力抑制剂”医学研究述评(Med.Res.Reviews)10：505-548)。对参与嘌呤和嘧啶代谢的酶的研究集中在研发可用作如免疫抑制剂或抗增生剂的新药上(Smith，J.L.，(1995)”核苷酸合成中的酶”，结构生物学最新观点(Curr.Opin.Struct.Biol.)5：752-757；(1995)Biochem Soc.Transact.23：877-902)。但是，嘌呤和嘧啶碱基、核苷和核苷酸具有其它可能应用：用作多种精细化学品(例如，硫胺素、S-腺苷-甲硫氨酸、叶酸盐或核黄素)生物合成的中间体，作为细胞的能量载体(例如，ATP或GTP)，和本身作为化学品，通常用作增味剂(例如，IMP或GMP)或用于一些医药用途(参见例如，Kuninaka，A.(1996)生物技术中的核苷酸和相关化合物(Nucleotides and Related Compounds inBiotechnology)第6卷，Rehm等人编辑.VCH：Weinheim，561-612页)。同样，参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作靶，研发用于作物保护的抗这些酶的化学品，包括杀真菌剂、除草剂和杀虫剂。
已阐释了这些化合物在细菌中的代谢(综述参见例如，核酸研究和分子生物学进展(Progress in Nucleic Acid Research and Molecular Biology)，第42卷，Academic Press中的Zalkin，H.和Dixon，J.E.(1992)”嘌呤核苷酸的从头生物合成”，259-287页；和生物化学途径：生物化学和分子生物学图集(Biochemical Pathways：An Atlas of Biochemistry and MolecularBiology)，Wiley：纽约一书中的Michal，G.(1999)”核苷酸和核苷”，第8章)。嘌呤代谢已是深入研究的主题，其对细胞的正常功能是必需的。高等动物中受损的嘌呤代谢可引起严重疾病，如痛风。嘌呤核苷酸从核糖-5-磷酸开始，经过一系列步骤，通过中间体化合物次黄苷-5’-磷酸(IMP)，产生鸟苷-5’-单磷酸(GMP)或腺苷-5’-单磷酸(AMP)，由此可以容易地形成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存体，这样它们的降解可为细胞内许多不同生物化学过程提供能量。嘧啶的生物合成由核糖-5-磷酸形成尿苷-5’-单磷酸(UMP)而进行。UMP接下来转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式通过一步还原步骤从核苷酸的二磷酸核糖形式产生为核苷酸的二磷酸脱氧核糖形式。通过磷酸化后，这些分子可参与DNA合成。
D.海藻糖的代谢和用途
海藻糖由两个葡萄糖分子通过α、α-1，1键连接组成。它通常作为甜味剂用于食品工业，或作为干的或冷冻食品和饮料中的添加剂。但它也用于药学、化妆品和生物技术工业(参见例如，Nishimoto等人(1998)美国专利号5,759,610；Singer，M.A.和Lindquist，S.生物技术趋势(TrendsBiotech.)16(1998)：460-467；Paiva，C.L.A.和Panek，A.D.Biotech.Ann.Rev.2(1996)：293-314；和Shiosaka，M.J.Japan 172：(1997)97-102)。海藻糖可以通过许多微生物的酶产生，并天然释放入环境介质中，从中可用本领域公知的方法分离海藻糖。
II.本发明基因的活性
对于一个特定的细菌物种，要在环境中生存必需有至少三种活性。首先，细胞必须能有效分裂，从而至少维持或增加细胞群体。其次，细胞必须能有效表达那些编码正常细胞功能所需蛋白的基因。最后，细胞必须有和环境相互作用的能力——通过适应主流环境条件；或通过生理运动至优选环境；或通过直接作用于环境从而提高其生存能力。涉及这些活性之任一的关键步骤包括细菌基因组复制、蛋白质生物合成中的核糖体活性、抗细胞或细胞裂解活性(因为它们涉及到一个生物体的发病机理)。
III.本发明元件和方法
本发明至少部分是基于新分子的检测，它们在本文中被称作RRP核酸分子。这些RRP核酸分子不仅适用于鉴定谷氨酸棒状杆菌或相关细菌种，而且还适于作为标记绘制谷氨酸棒状杆菌基因组图谱，以及鉴定适合如通过发酵工艺生产精细化学品的细菌。本发明还至少部分是基于所述RRP核酸分子编码的RRP-蛋白分子。这些RRP分子能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率，作为谷氨酸棒状杆菌或相关生物体的鉴定标记，降解碳氢化合物和作为研发治疗性药学化合物的靶标。在一个实施方案中，本发明的RRP分子直接或间接地参与谷氨酸棒状杆菌中一种或多种精细化学品的代谢途径。在优选的实施方案中，本发明的RRP分子直接或间接地参与此类代谢途径的活性对所述微生物中目的精细化学品的生产具有影响。在尤其优选的实施方案中，本发明的RRP分子的活性被调节，由此调节本发明的RRP蛋白所参与的谷氨酸棒状杆菌代谢途径的效率或产出，而这又直接或间接地调节谷氨酸棒状杆菌中目的精细化学品的产量或生产效率。
术语“RRP蛋白”或“RRP多肽”包括可调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物以及作为药物筛选或药物设计的靶标蛋白、或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记的蛋白。RRP蛋白的例子包括那些由表1和集合A中列出的RRP基因编码的蛋白。术语“RRP基因”或“RRP核酸序列”包括编码RRP蛋白、含有编码区和相应的非翻译的5’和3’序列区的核酸序列。RRP基因的实例在表1中列出。
术语“产量”和“生产力”是本领域中已知的并且包括发酵产物的浓度(例如，在预定的时间段和预定的发酵体积内生产的目的精细化学品的浓度(例如kg产物/h/l))。术语“生产效率”包括细胞为得到特定产物量所需的时间(例如，细胞为达到精细化学品的特定产出速率所需的时间)。术语“产率”或“产物/碳产率”是本领域已知的并且包括将碳源转化成产物(即精细化学品)的效率。这例如通常表达为kg产物/kg碳源。增加化合物的产率或产量可在预定时间内在特定培养体积中增加所得分子或该化合物的适宜得到的分子的量。术语“生物合成”和“生物合成途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中从中间物合成化合物，优选有机化合物。术语“降解”和“降解途径”是本领域中已知的并且包括细胞在例如多步过程或高度调节的过程中将化合物，优选有机化合物切割成降解产物(用更通俗的术语：更小或更简单的分子)。术语“代谢”是本领域中已知的并且包括生物体中发生的生物化学反应的整体。因此，特定化合物的代谢(例如氨基酸如甘氨酸的代谢)包括细胞中影响该化合物的所有生物合成、修饰和降解途径。
在另一个优选的实施方案中，本发明的RRP分子能够直接或间接地调节微生物如谷氨酸棒状杆菌中目的分子如精细化学品的生产。利用基因重组技术，可以操作一种或多种本发明的RRP蛋白使它们的功能受到调节。这种功能调节可以导致对来自谷氨酸棒状杆菌的一种或多种精细化学品的产率、产量和/或生产效率的调节。
例如，可以通过修饰参与精细化学品的生物合成或降解的蛋白的活性(即通过相应基因的突变)，直接地调节细胞合成或降解所述化合物的能力，由此调节该精细化学品的产率和/或生产效率。同样，可以通过调节调控精细化学品代谢途径的蛋白的活性，直接影响目的化合物的产量是上调还是下调，这两种情况都将调节来自细胞的精细化学品的产率或生产效率。
通过修饰本发明蛋白的活性(即突变相应基因)从而整体提高细胞生长、分裂或保持存活及生产力的能力，也可间接调节精细化学品的生产。精细化学品通常从大规模发酵培养的谷氨酸棒状杆菌中产生，而大规模发酵培养这些微生物的条件对该微生物的生长和细胞分裂常常不是最适宜的。通过修饰本发明的蛋白(如应激反应蛋白，细胞壁蛋白或涉及细胞生长和细胞分裂中所必需的化合物，诸如核苷酸和氨基酸的代谢的蛋白)以改良这些条件下微生物的存活、生长和繁殖，这可能在大规模培养中提高这些修饰过的谷氨酸棒状杆菌细胞的数量和生产力，这将反过来导致一种或多种目的精细化学品的产率和/或生产效率的提高。此外，细胞代谢途径必然地彼此相依并被共同调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性)，可以同时改变或者调节这个微生物中另一代谢途径的活性，而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
本发明分离的核酸序列定位于可从美国典型培养物保藏中心(American Type Culture Collection)获得的谷氨酸棒状杆菌菌株的基因组中，该菌株的保藏号为ATCC 13032。分离的谷氨酸棒状杆菌RRP核酸分子的核苷酸序列和预测的该谷氨酸棒状杆菌RRP蛋白的氨基酸序列分别如集合A和集合B所示。进行计算分析，已将这些核苷酸序列中的许多归类于和/或鉴定为与大肠杆菌(E.coli)或枯草芽孢杆菌(Bacillussubtilis)基因具有同源性的序列。
本发明也涉及这样的蛋白质，其具有实质上与集合B中的氨基酸序列同源的氨基酸序列。如本文所用，所具有的氨基酸序列与所选氨基酸序列实质上同源的蛋白，将至少约50％同源于所选氨基酸序列，例如所选完整氨基酸序列。所具有的氨基酸序列与所选氨基酸序列实质上同源的蛋白也可至少约50-60％，优选地至少约60-70％，且更优选地至少约70-80％、80-90％、或90-95％，且最优选地至少约96％、97％、98％、99％或甚至更高地同源于所选的氨基酸序列。
本发明的RRP蛋白或其生物活性部分或其片段能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率，或降解碳氢化合物，或氧化萜类化合物，或作为药物研发的靶标或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
下面的小章节更详细地描述了本发明的各个方面：
A.分离的核酸分子
本发明的一个方面涉及编码RRP多肽或其生物活性部分的分离核酸分子及核酸片段，其中所述核酸片段足以用作杂交探针或引物，用来鉴定或扩增编码RRP的核酸(例如RRP DNA)。这些核酸分子可用于鉴定谷氨酸棒状杆菌或相关生物体，用于绘制谷氨酸棒状杆菌或相关生物体的基因组图谱，或者用于鉴定适于例如通过发酵工艺产生精细化学品的微生物。如此处所用，术语“核酸分子”是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)和用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’和5’端的非翻译区：基因编码区5’端上游的至少约100个核苷酸的序列和基因编码区3’端下游的至少约20个核苷酸的序列。核酸分子可为单链或双链，但优选地为双链DNA。“分离的”核酸分子为从存在于此核酸的天然源中的其它核酸分子中取出的核酸分子。优选地，“分离的”核酸不具有在该核酸所来自的生物体基因组DNA中天然存在于该核酸侧翼的序列(例如，位于该核酸5’或3’端的序列)。在不同实施方案中，该分离的RRP核酸分子可包含例如，少于约5kb，4kb，3kb，2kb，1kb，0.5kb或0.1kb在该核酸所来自的细胞(如谷氨酸棒状杆菌细胞)基因组DNA中天然存在于该核酸分子侧翼的核苷酸序列。而且，“分离的”核酸分子如cDNA分子当通过重组技术产生时可基本上不含有其它细胞物质或培养基，当通过化学合成产生时，可基本上不含有化学前体或其它化学品。
本发明的核酸分子，如具有集合A的核苷酸序列的核酸分子或其部分可用标准的分子生物学技术和本文提供的序列信息分离。例如，使用集合A的完整或部分序列作为杂交探针和标准的杂交技术(例如，在Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，冷泉港实验室，冷泉港实验室出版社，冷泉港，纽约，1989中所述)，可自谷氨酸棒状杆菌文库分离谷氨酸棒状杆菌RRP cDNA。而且，包含集合A的完整序列或其部分的核酸分子可使用根据该序列设计的寡核苷酸引物，应用聚合酶链反应分离(例如，包含集合A的完整或部分序列的核酸分子可使用根据集合A的该同样序列产生的寡核苷酸引物，应用聚合酶链反应分离)。例如，可从正常的内皮细胞分离mRNA(例如，通过Chirgwin等人(1979)生物化学(Biochemistry)18：5294-5299的硫氰酸胍提取法)，并用反转录酶(例如，莫洛尼鼠类白血病病毒反转录酶，可从Gibco/BRL，Bethesda，MD获得；或AMV反转录酶，可从Seikagaku America，Inc.，St.Petersburg，FL获得)和随机多核苷酸引物或基于集合A中所示的任一核苷酸序列的寡核苷酸引物制备cDNA。用于聚合酶链反应扩增的合成寡核苷酸引物可根据集合A所述的核苷酸序列之一产生。本发明的核酸可用cDNA或用基因组DNA作为模板、使用合适的寡核苷酸引物按照标准的PCR扩增技术来扩增。这样扩增的核酸可克隆入合适的载体并通过DNA序列分析鉴定。对应于RRP核苷酸序列的寡核苷酸还可以通过标准的合成技术制备，例如用DNA自动合成仪制备。
在一个优选的实施方案中，本发明的分离核酸分子包含集合A中所列的核苷酸序列之一。集合A的序列对应于本发明的来自谷氨酸棒状杆菌的RRP cDNA。这些cDNA包含RRP蛋白序列(即在集合A的每一序列中所指出的“编码区”)以及同样在集合A中指出的5’和3’的非翻译区。作为备选方案，核酸分子可以只包含集合A中核苷酸序列之一的编码区。
除此之外，本发明的核酸分子可以只包含集合A中核苷酸序列之一的编码区的一部分，例如可用作探针或引物的片断，或者编码RRP蛋白的生物活性部分的片断。从克隆谷氨酸棒状杆菌RRP基因确定的核苷酸序列使得可以产生设计用于鉴定和/或克隆其它细胞类型和生物体中的RRP同系物以及来自其它棒状杆菌或相关种的RRP同系物的探针和引物。探针或引物通常包含基本上纯的寡核苷酸。该寡核苷酸通常包含在严谨条件下可以与集合A中所示的核苷酸序列之一的有义链、集合A中所示的核苷酸序列之一的反义链或其天然存在的突变体中的至少约12个，优选地约25个，更优选地约40、50或75个连续核苷酸杂交的核苷酸序列区域。基于集合A的核苷酸序列的引物可用于PCR反应中以克隆RRP同系物。基于RRP核苷酸序列的探针可用于检测编码相同或同源蛋白的转录本或基因组序列。在优选的实施方案中，该探针还包含与其连接的标记基团，例如放射性同位素、荧光化合物、酶或酶辅因子。这些探针可用作诊断试验试剂盒的一部分用来鉴定误表达RRP蛋白的细胞，例如测定细胞样品中RRP编码核酸的量，例如检测RRP mRNA水平，或确定基因组RRP基因是否已突变或已缺失。
在一个实施方案中，本发明的核酸分子编码这样的蛋白或其部分，其包括的氨基酸序列充分同源于集合B的氨基酸序列，以至于该蛋白或其部分仍然保留调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记的能力。如本文所用，术语“充分同源”是指蛋白或其部分所具有的氨基酸序列包括最小数目的与集合B的氨基酸序列相同或等同的氨基酸残基(例如，氨基酸残基具有与集合B中序列之一的氨基酸残基相似的侧链)，以至于该蛋白或其部分能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或者作为谷氨酸棒状杆菌或相关生物体的鉴定标记。这些活性的实例也在本文进行了描述。因此，“RRP蛋白的功能”有助于一种或多种精细化学品的代谢途径的总体调节，或碳氢化合物的降解，或萜类化合物的氧化。
本发明RRP核酸分子编码的蛋白的部分优选是任何RRP蛋白的生物学活性部分。如本文所用的术语“RRP蛋白的生物学活性部分”旨在包括RRP蛋白的一部分，例如结构域或基元，其能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、以及作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记。为了确定RRP蛋白或者其生物学活性部分能否调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和生产效率、降解碳氢化合物或氧化萜类化合物，可以进行酶活性分析。这些分析方法在实施例部分的实施例8中详细描述，且技术人员熟知这些方法。
其它编码RRP蛋白生物活性部分的核酸片段可通过分离集合B中序列之一的部分、表达所编码的RRP蛋白或RRP肽的部分(例如，通过体外重组表达)并测定所编码的RRP蛋白或RRP肽的部分的活性而制备。
而且，本发明包括由于遗传密码的简并性而不同于集合A所示的核苷酸序列之一(及其部分)，并由此与集合A所示的此核苷酸序列编码相同的RRP蛋白的核酸分子。在另一实施方案中，本发明的分离核酸分子具有这样的核苷酸序列，其编码的蛋白具有如集合B中所示的氨基酸序列。在进一步的实施方案中，本发明的核酸分子编码全长的谷氨酸棒状杆菌蛋白，而该蛋白基本上同源于集合B中所示的氨基酸序列(由集合A中所示的可读框编码)。
除了群体中可能存在的RRP序列的天然变体外，技术人员还理解可通过突变向集合A的核苷酸序列中导入变化，由此导致所编码的RRP蛋白的氨基酸序列改变，但不损害所述RRP蛋白的功能。例如，可在集合A的核苷酸序列中进行在“非必需”氨基酸残基处导致氨基酸替换的核苷酸替换。“非必需”氨基酸残基是任一野生型RRP蛋白序列(集合B)中可以被修饰而不改变所述RRP蛋白的活性的残基，但“必需”氨基酸残基为RRP蛋白活性所需。然而，其它氨基酸残基(例如，在具有RRP活性的结构域中不保守或仅为半保守的那些氨基酸残基)对活性可能为非必需的，并因此可能被改变而不导致RRP活性的改变。
因此，本发明的另一个方面涉及这样的核酸分子，其编码的RRP蛋白含有对于RRP活性非必需的修饰的氨基酸残基。这些RRP蛋白的氨基酸序列与集合B中的序列不同，但尽管如此，该蛋白保留至少一种本文所述的RRP活性。在一个实施方案中，该分离的核酸分子包含编码蛋白的核苷酸序列，其中该蛋白包含至少约50％同源于集合B中的氨基酸序列的氨基酸序列，并且该蛋白能够调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率、降解碳氢化合物、氧化萜类化合物、作为药物研发的靶标或作为谷氨酸棒状杆菌或相关生物体的鉴定标记。
编码同源于集合B的蛋白质序列的RRP蛋白的分离核酸分子可通过将一个或多个核苷酸替换、添加或缺失导入集合A的核苷酸序列中以使一个或多个氨基酸替换、添加或缺失导入所编码的蛋白质中而产生。可通过标准技术将突变导入集合A的核苷酸序列之一，如定点诱变和PCR-介导的诱变。优选地，在一处或多处预测的非必需氨基酸残基处进行保守氨基酸替换。“保守氨基酸替换”为将氨基酸残基用具有相似侧链的氨基酸残基替换。具有相似侧链的氨基酸残基家族已在本领域定义过。这些家族包括具有碱性侧链的氨基酸(例如，赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如，天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如，甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如，丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如，苏氨酸、缬氨酸、异亮氨酸)和具有芳族侧链的氨基酸(例如，酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此，优选地对RRP蛋白中预测的非必需氨基酸残基用来自同一侧链家族的另一氨基酸残基替换。或者，在另一实施方案中，可通过如饱和诱变沿着全部或部分RRP编码序列随机导入突变，并根据文中所述的RRP活性对所得突变体进行检测，以鉴定保留RRP活性的突变体。对集合A序列之任一诱变后，可重组表达编码蛋白，并用例如文中所述的试验(参见实施例部分的实施例8)确定所述蛋白质的活性。
B.重组表达载体和宿主细胞
本发明的另一方面涉及包含编码RRP蛋白(或其部分)的核酸的载体，优选表达载体。如此处所用，术语”载体”是指这样的核酸分子，其可转运与其连接的另一核酸。一种类型的载体为”质粒”，是指其中可连接额外DNA片段的环状双链DNA环。另一种类型的载体为病毒载体，其中可将额外DNA片段连接入病毒基因组。某些载体可在它们所导入的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体和游离型哺乳动物载体)。其它载体(例如，非游离型哺乳动物载体)在导入宿主细胞后整合入宿主细胞的基因组，并因此随着宿主基因组而复制。而且，某些载体可指导与它们功能性连接的基因的表达。此类载体此处称为”表达载体”。一般，可以用于重组DNA技术中的表达载体为质粒形式。在本说明书中，”质粒”和”载体”可互换使用，因为质粒是最经常使用的载体形式。但是，本发明旨在包括其它形式的表达载体，如病毒载体(例如，复制缺陷型反转录病毒、腺病毒和腺相关病毒)，它们起到类似的作用。
本发明的重组表达载体以适于在宿主细胞中表达本发明核酸的形式包含本发明的核酸，这就是说重组表达载体包括一个或多个调节序列，这些调节序列基于表达所用的宿主细胞进行选择并功能性地与待表达的核酸序列连接。在重组表达载体中，术语“功能性连接”是指目的核苷酸序列与调节序列以允许该核苷酸序列表达(例如，在体外转录/翻译系统中，或当载体导入宿主细胞时在宿主细胞中表达)的方式连接。术语“调节序列”旨在包括启动子、阻遏物结合位点、激活物结合位点、增强子区和其它表达调控元件(例如，终止子、mRNA二级结构的其它元件或者多聚腺苷酸化信号)。此类调节序列如在Goeddel；基因表达技术：酶学方法(GeneExpressionTechnology：Methods in Enzymology)185，Academic Press，SanDiego，CA(1990)中描述。调节序列包括控制核苷酸序列在许多类型宿主细胞中表达的调节序列和控制核苷酸序列仅在特定宿主细胞中表达的调节序列。本领域普通技术人员了解，表达载体的设计可取决于如对欲转化宿主细胞的选择、所需的蛋白质表达程度等因素。本发明的表达载体可导入宿主细胞，以产生由此处所述核酸所编码的蛋白质或肽，包括融合蛋白或融合肽(例如，RRP蛋白、RRP蛋白的突变形式、融合蛋白等)。
本发明的重组表达载体可设计用于在原核或真核细胞中表达RRP蛋白。例如，RRP基因可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母细胞和其它真菌细胞(参见Romanos，M.A.等人(1992)“在酵母中外源基因的表达：综述”(”Foreign gene Expression inyeast：a review”)，酵母(Yeast)8：423-488；J.W.Bennet和L.L.Lasure编辑的《更多的用于真菌的基因操作》(More Gene Manipulations inFungi)，Academic Press：San Diego一书中的van den Hondel，C.A.M.J.J.等人(1991)“丝状真菌中的异源基因表达”(”Heterologous geneExpression in filamentous fungi”)，396-428页；以及Peberdy，J.F.等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi)，Cambridge University Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfer systems and vector development for filamentous fungi)，1-28页)、藻类和多细胞植物细胞(参见Schmidt，R.和Willmitzer，L.(1988)，“根癌农杆菌介导的拟南芥叶和子叶外植体的高效转化”，植物细胞报道(Plant Cell Rep.)：583-586)或哺乳动物细胞中表达。合适的宿主细胞在Goeddel，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，CA(1990)中进一步进行了讨论。备选地，可体外转录并翻译重组表达载体，如使用T7启动子调节序列和T7聚合酶。
在原核生物中蛋白的表达主要用含有组成型或诱导型启动子的载体进行，以控制融合或非融合蛋白的表达。融合载体将一些氨基酸添加至其中编码的蛋白上，通常添加至重组蛋白的氨基末端。这些融合载体一般具有三个任务：1)用于增强重组蛋白的表达；2)用于增加重组蛋白的溶解性；和3)在亲和纯化中作为配体而利于重组蛋白的纯化。通常，将蛋白酶剪切位点于融合单元和重组蛋白的连接处导入到融合表达载体中，这使得在该融合蛋白纯化后，可将重组蛋白与融合单元分离。这些酶和它们的对应识别序列包括Xa因子、凝血酶和肠激酶。
通常的融合表达载体包括pGEX(Pharmacia Biotech Inc；Smith，D.B.和Johnson，K.S.(1988)基因(Gene)67：31-40)、pMAL(New EnglandBiolabs，Beverly，MA)和pRIT5(Pharmacia，Piscataway，NJ)，它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白和蛋白A融合至重组靶蛋白。在一个实施方案中，将RRP蛋白的编码序列克隆入pGEX表达载体，以产生编码融合蛋白的载体，该蛋白从N-末端至C-末端包含GST-凝血酶切割位点-X蛋白。该融合蛋白可以使用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。通过将该融合蛋白用凝血酶切割可得到不与GST融合的重组RRP蛋白。
合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等人(1988)基因(Gene)69：301-315)和pET 11d(Studier等人，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)60-89)。pTrc载体中靶基因的表达依赖于宿主RNA聚合酶从杂合的trp-lac融合启动子的转录。pET11d载体中靶基因的表达依赖于共表达的病毒RNA聚合酶(T7 gn1)介导的从T7-gn10-lac融合启动子的转录。该病毒聚合酶可以由宿主菌株BL21(DE3)或HMS174(DE3)中定居的、具有位于lacUV 5启动子转录调控下的T7 gn1基因的λ原噬菌体提供。
最大化重组蛋白表达的一个策略是将该蛋白在对所述重组蛋白进行蛋白酶切割的能力被破坏的宿主细菌中表达(Gottesman，S.，基因表达技术：酶学方法(Gene Expression Technology：Methods in Enzymology)185，Academic Press，San Diego，California(1990)119-128)。另一策略是改变欲插入表达载体的核酸的核酸序列，以使编码每一氨基酸的各密码子为选择用于表达的细菌如谷氨酸棒状杆菌所优选使用的密码子(Wada等人(1992)核酸研究(Nucleic Acids Res.)20：2111-2118)。本发明核酸序列的这种改变可应用DNA合成的标准技术实施。
在另一实施方案中，RRP蛋白表达载体为酵母表达载体。用于在酵母菌酿酒酵母(S.cerevisiae)中表达的载体的实例包括pYepSec1(Baldari，等人(1987)Embo J.6：229-234)、pMFa(Kurjan和Herskowitz，(1982)细胞(Cell)30：933-943)，pJRY88(Schultz等人(1987)基因(Gene)54：113-123)和pYES2(Invitrogen Corporation，San Diego，CA)。适用于其它真菌如丝状真菌的载体和用于构建载体的方法包括在：J.F.Peberdy等人编辑的《真菌的应用分子遗传学》(Applied Molecular Genetics of Fungi)，CambridgeUniversity Press：Cambridge一书中的van den Hondel，C.A.M.J.J.和Punt，P.J.(1991)“丝状真菌的基因转移体系和载体开发”(”Gene transfersystems and vector development for filamentous fungi)，1-28页中详述的那些。
作为另一备选方案，本发明的RRP蛋白可使用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf 9细胞)中表达蛋白的杆状病毒载体包括pAc系列(Smith等人(1983)Mol.Cell Biol.3：2156-2165)和pVL系列(Lucklow和Summers(1989)病毒学(Virology)170：31-39)。
在另一实施方案中，本发明的RRP蛋白可在单细胞植物细胞(如藻类)或在来自高等植物(如种子植物，诸如作物)的植物细胞中表达。植物表达载体的实例包括在：Becker，D.，KemPer，E.，Schell，J.和Masterson，R.(1992)“具有靠近左边界的选择性标记的新植物双元载体”(”New plantbinary vectors with selectable markers located proximal to the leftborder”)，植物分子生物学(Plant Mol.Biol.)20：1195-1197；和Bevan，M.W(1984)“用于植物转化的双元农杆菌载体”(”Binary Agrobacteriumvectors for plant transformation”)，核酸研究(Nucl.Acid.Res.)12：8711-8721中详述的那些。
在另一实施方案中，本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed，B.(1987)自然(Nature)329：840)和pMT2PC(Kaufman等人(1987)EMBO J.6：187-195)。当用于哺乳动物细胞时，表达载体的调控功能通常由病毒调控元件提供。例如，常用的启动子来自多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。用于原核和真核细胞的其它合适表达系统参见Sambrook，J.，Fritsh，E.F.和Maniatis，T.分子克隆：实验室手册(Molecular Cloning：ALaboratory Manual)，第二版，Cold Spring Harbor Laboratory，ColdSpring Harbor Laboratory Press，Cold Spring Harbor，NY，1989中的第16和17章。
在另一实施方案中，重组哺乳动物表达载体可以导致核酸优选地在特定细胞类型中表达(例如，使用组织-特异性调控元件来表达核酸)。组织-特异性调控元件为本领域公知。合适的组织-特异性启动子的非限制性实例包括白蛋白启动子(肝特异性；Pinkert等人(1987)基因进展(Genes Dev.)1：268-277)、淋巴特异性启动子(Calame and Eaton(1988)Adv.Immunol.43：235-275)，特别是T细胞受体启动子(Winoto和Baltimore(1989)EMBOJ.8：729-733)和免疫球蛋白启动子(Banerji等人(1983)细胞(Cell)33：729-740；Queen和Baltimore(1983)细胞(Cell)33：741-748)、神经元特异性启动子(例如，神经丝启动子；Byrne和Ruddle(1989)PNAS86：5473-5477)、胰特异性启动子(Edlund等人(1985)科学(Science)230：912-916)以及乳腺特异性启动子(例如，milk serum启动子；美国专利No.4,873,316和欧洲专利申请文件No.264,166)。同样，还包括发育调节的启动子，如鼠hox启动子(Kessel和Gruss(1990)科学(Science)249：374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)基因进展(Genes Dev.)3：537-546)。
而且，本发明提供了含本发明的DNA分子的重组表达载体，其中该DNA分子以反义方向克隆在表达载体中。也就是，DNA分子功能性地连接到调控序列上，以至于可以表达(通过转录该DNA分子)产生与RRPmRNA反义的RNA分子。可以对功能地与反义方向克隆的核酸连接的调控序列进行选择以控制反义RNA分子在多种细胞类型中持续表达；例如，可选择病毒启动子和/或增强子或调控序列以控制反义RNA的组成型组织特异性或细胞类型特异性表达。反义表达载体的形式可为重组质粒、噬菌粒或减毒病毒，其在高效调控区的控制下产生反义核酸，而所述调控区的活性由载体所导入的细胞的类型决定。关于利用反义基因实施基因表达调控的讨论见Weintraub，H.等人，反义RNA作为遗传分析的分子工具，Reviews-Trends in Genetics，Vol.1(1)1986。
本发明的另一方面涉及已导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在此处可互换使用。当然，该术语不仅指特定的靶细胞，也指该细胞的后代或潜在后代。由于突变或环境因素细胞在传代过程中可发生某些改变，这样，后代可能并不一定与亲代细胞相同，但其仍包括在此处所用术语的范围之内。
宿主细胞可为原核或真核细胞。例如，RRP蛋白可在细菌细胞如谷氨酸棒状杆菌、昆虫细胞、酵母或哺乳动物细胞(如中国仓鼠卵巢(CHO)细胞或COS细胞)中表达。其他合适的宿主细胞为技术人员公知。与谷氨酸棒状杆菌有关的、能够以适宜的方式用作本发明核酸和蛋白分子的宿主细胞的微生物如表3所示。
载体DNA可通过常规转化或转染技术导入原核或真核细胞中。如本文所用，术语“转化”和“转染”、“接合”和“转导”旨在包括本领域公知的各种将外源核酸(例如DNA)导入宿主细胞的技术，包括天然感受态、化学介导的转移、磷酸钙或氯化钙共沉淀、DEAE-葡聚糖-介导的转染、脂转染或电穿孔。转化或转染宿主细胞的合适方法可在Sambrook等人(分子克隆：实验室手册(Molecular Cloning：A Laboratory Manual).第二版，ColdSpring Harbor Laboratory，Cold Spring Harbor Laboratory Press，ColdSpring Harbor，NY，1989)和其它的实验室手册中找到。
对于哺乳动物细胞的稳定转染，已知根据所用的表达载体和所用的转染技术，仅一小部分的细胞可将外源DNA整合到基因组中。一般将编码选择标记(如抗生素抗性)的基因与目的基因一同导入宿主细胞以鉴定和筛选这些整合体。优选的选择标记包括那些可赋予药物抗性的基因，所述药物如G418、潮霉素和氨甲蝶呤。编码选择标记的核酸可与编码RRP蛋白的基因位于同一载体上被导入宿主细胞或可以位于不同的载体上导入。由已导入的核酸稳定转染的细胞可通过例如药物选择进行鉴定(例如，整合有选择标志基因的细胞将存活，而其它细胞死亡)。
通过制备出含至少RRP基因一部分的载体——所述RRP基因一部分中已经导入缺失、添加或替代——可以生成同源重组微生物，由此改变RRP基因(如功能性破坏)。优选地该RRP基因为谷氨酸棒状杆菌RRP基因，但也可以利用来自相关细菌或甚至来自哺乳动物、酵母或昆虫来源的同系物。在优选的实施方案中，载体设计为在同源重组后可功能性破坏内源RRP基因(即，该基因不再编码功能蛋白；同样地也称为“敲除”载体)。作为备选方案，载体可设计为在同源重组后使内源RRP基因发生突变或以其它方式改变但仍编码功能蛋白(例如，可改变位于上游的调控区由此改变内源RRP蛋白的表达)。在此同源重组载体中，改变的RRP基因部分在其5′和3′端侧翼为RRP基因的其它核酸，从而使得载体所携带的外源RRP基因和微生物中的内源RRP基因之间可以发生同源重组。此额外的侧翼RRP核酸的长度应足以使与内源基因的同源重组成功进行。通常，载体内包括不到一千碱基的侧翼DNA(5′及3′端)(参见例如，Thomas，K.R.，和Capecchi，M.R.(1987)细胞(Cell)51：503中对同源重组载体的描述)。将该载体导入微生物(例如，通过电穿孔)，并使用本领域公知的方法，筛选内源RRP基因已与导入的RRP基因同源重组的细胞。
在另一实施方案中，产生的重组微生物含有可调控导入的基因的表达的选定系统。例如，将RRP基因插入载体中位于乳糖操纵子的控制下，可以使RRP基因仅在存在IPTG时表达。这些调控系统为本领域熟知。
本发明的宿主细胞如培养的原核或真核宿主细胞可用于产生(即表达)RRP蛋白。而且，本发明还提供了使用本发明的宿主细胞来产生RRP蛋白的方法。在一个实施方案中，该方法包括在合适的培养基中培养本发明的宿主细胞(其中已导入了编码RRP蛋白的重组表达载体，或其基因组中已导入了编码野生型或改变的RRP蛋白的基因)，直至产生RRP蛋白。在另一实施方案中，该方法还包括从培养基或宿主细胞分离RRP蛋白。
C.本发明的用途和方法
文中所述的核酸分子、蛋白、蛋白同系物、融合蛋白、引物、载体和宿主细胞可用于下述一种或多种方法中：鉴定谷氨酸棒状杆菌及相关生物体，绘制与谷氨酸棒状杆菌相关的生物体的基因组图谱，鉴定并定位目的谷氨酸棒状杆菌序列，进化研究，确定RRP蛋白中功能所需的RRP蛋白区域，调节RRP蛋白的活性，调节一种或多种代谢途径的活性，以及调节目的化合物如精细化学品的细胞生产。本发明的RRP核酸分子具有多种用途。首先，它们可用于鉴定生物体是否为谷氨酸棒状杆菌或其近亲。它们也可用于在混合的微生物群体中鉴定谷氨酸棒状杆菌或其相关菌。本发明提供了许多谷氨酸棒状杆菌基因的核酸序列。通过将从微生物单一群体或混合群体的培养物提取的基因组DNA在严谨条件下用包含对谷氨酸棒状杆菌而言独特的谷氨酸棒状杆菌基因区域的探针来探测，可确定该生物体是否存在。虽然谷氨酸棒状杆菌自身为非致病的，但它与致病种如白喉棒状杆菌有亲缘关系。这种生物体的检测具有实质上的临床重要性。
通过利用本领域公知的技术可检测样品中谷氨酸棒状杆菌的存在。特别地，可以首先在合适的液体或合适的固体培养基中培养样品中的细胞，以增加培养物中细胞的数目。裂解这些细胞并提取其中所含的全部DNA，并且在适当的时候，进行纯化以除去可能干扰随后分析的细胞碎片和蛋白物质。实施本领域公知的聚合酶链式反应或相似技术(有关核酸序列扩增通常所用的方法学的一般概述，参见Mullis等人，美国专利No.4683195，Mullis等人，美国专利No.4965188以及Innis，M.A.，und Gelfand，D.H.(1989)PCR方法，方法及应用指南(PCR-Protocols，A guide to Methodsand Applications)，Academic Press，pp.3-12，和(1988)生物技术(Biotechnology)6：1197，以及国际专利申请No.WO89/01050)，其中，由对本发明的RRP核酸分子特异的引物与样品核酸温育，从而使所述的特定RRP核酸序列，如果存在于样品中的话，得以扩增。待扩增的特定核酸序列的选择依据是它唯一性地存在于谷氨酸棒状杆菌以及仅有的少数近亲细菌的基因组中。期望的扩增产物的存在可指示谷氨酸棒状杆菌或与谷氨酸棒状杆菌密切相关的生物体的存在。
本发明的核酸和蛋白分子也可用作基因组特定区域的标记。利用本领域公知的技术，可以检测本发明的RRP核酸分子在谷氨酸棒状杆菌基因组中的物理定位，而这接下来可用来更容易地在图谱中定位其它核酸分子和基因。而且，本发明的核酸分子可能与相关种的序列充分同源，以至于这些核酸分子同样能够在此类细菌(如乳糖发酵短杆菌BrevibacteriumLactofermentum))中构建基因组图谱。
本发明的核酸和蛋白分子不仅适于绘制基因组图谱，也适用于谷氨酸棒状杆菌蛋白的功能研究。例如，通过切割谷氨酸棒状杆菌基因组，并将片段与DNA-结合蛋白温育，可鉴定与特定的谷氨酸棒状杆菌DNA-结合蛋白结合的基因组区域。另外，那些结合蛋白的片段可用本发明的核酸分子探测，优选地使用易于检测的标记物；此核酸分子与基因组片段的结合可将片段定位于谷氨酸棒状杆菌的基因组图谱中，并且当用不同的酶重复几次时，将有利于快速确定蛋白结合的核酸序列。
本发明的RRP核酸分子同样也适用于进化研究和蛋白结构研究。许多原核和真核细胞应用本发明分子参与的代谢过程；通过将本发明核酸分子的序列与那些来自其它生物体的编码类似酶的序列进行比较，可确定所述生物体的进化相关程度。由此，此比较使得可确定哪些序列保守以及哪些不保守，而这有利于确定对酶功能所必需的那些蛋白区域。这种确定对蛋白工程研究有价值，并可指示蛋白可耐受多少诱变而不丧失其功能。
本发明的RRP蛋白可用作为标记将未知细菌归类为谷氨酸棒状杆菌或鉴定样品中的谷氨酸棒状杆菌或近亲细菌。例如，利用本领域公知的技术，在适当的时候，可以扩增样品中的细胞(例如通过在合适的培养基中培养)，以增加样品的大小，然后裂解所述细胞，由此释放其中所含的蛋白。在适当的时候，该样品可以进行纯化，以除去可能会干扰随后的分析的细胞碎片和核酸分子。选定的本发明的RRP蛋白的特异性抗体可以在典型的Western试验形式中与蛋白样品进行温育(例如参见Ausubel等人，(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：纽约)，这样如果所述蛋白存在于样品中的话，抗体与它的靶蛋白结合。如果一个RRP蛋白对谷氨酸棒状杆菌或谷氨酸棒状杆菌及其亲缘关系很近的细菌而言是独特的或几乎是独特的，则该RRP蛋白被选择用于此类试验。样品中的蛋白随后通过凝胶电泳分级分离，并转移到合适的基质如硝酸纤维素上。带有可检测的标记(如化学发光或显色的)的合适的二抗与该基质温育，接着进行严格洗涤。标记的存在或不存在指示了样品中靶蛋白的存在或不存在。如果蛋白存在，则这表明谷氨酸棒状杆菌的存在。相似的方法使得可以将未知细菌归类为谷氨酸棒状杆菌；如果许多谷氨酸棒状杆菌特异性的蛋白在从未知细菌制备的蛋白样品中均未检测到，则该细菌很可能不是谷氨酸棒状杆菌。
对本发明RRP核酸分子的遗传操作可导致产生与野生型RRP蛋白功能不同的RRP蛋白。这些蛋白可在功效或活性上提高，可以在细胞内以比通常多的量存在，或可以在功效或活性上降低。
这些活性的改变可以直接调节谷氨酸棒状杆菌中一种或多种精细化学品的产率、产量和/或生产效率。例如，有可能通过修饰参与精细化学品的生物合成或降解的蛋白的活性(即通过相应基因的突变)，直接调节细胞合成或降解所述化合物的能力，由此调节该精细化学品的产率和/和生产效率。同样地，有可能通过调节调控精细化学品代谢途径的蛋白的活性，直接影响目的化合物的产生是上调还是下调，两种方式都将调节来自细胞的精细化学品的产率或生产效率。
通过修饰本发明蛋白的活性(即突变相应基因)，从而整体提高细胞生长、分裂或保持存活及生产力的能力，也可间接调节精细化学品的生产。精细化学品通常从大规模发酵培养的谷氨酸棒状杆菌中产生，而大规模发酵培养这些微生物的条件对该微生物的生长和细胞分裂常常不是最适宜的，可以通过修饰本发明的蛋白(如应激反应蛋白，细胞壁蛋白或涉及细胞分裂和细胞生长中所必需的化合物，诸如核苷酸和氨基酸的代谢的蛋白)使所述培养条件下细胞可更好存活，生长和繁殖，从而在大规模培养中提高这些修饰过的谷氨酸棒状杆菌细胞的数量和生产力，这将反过来导致一种或多种目的精细化学品的产率和/或生产效率的提高。此外，细胞代谢途径必然地彼此相依并被共同调控。改变谷氨酸棒状杆菌任一代谢途径的活性(即改变参与此类途径的本发明蛋白之一的活性)，可以同时改变或者调节这个微生物中另一代谢途径的活性，而此另一代谢途径可能直接地涉及精细化学品的合成或降解。
前述的这些突变RRP蛋白以增加谷氨酸棒状杆菌中精细化学品的产率的策略并不旨在限制；这些突变策略的变通方案对技术人员是相当明显的。使用这些策略且包括本文公开的机制，使得可以使用本发明的核酸和蛋白分子产生表达突变的RRP核酸和蛋白分子的谷氨酸棒状杆菌或者相关细菌菌株，从而提高目的化合物的产率、产量和/或生产效率。目的化合物可以是谷氨酸棒状杆菌的任何产物，包括生物合成途径的终产物和天然代谢途径的中间产物以及非天然存在于谷氨酸棒状杆菌代谢中的但是被本发明谷氨酸棒状杆菌菌株生产的分子。
以下实施例进一步举例阐释本发明，其不当理解为限制性的。在本专利申请中引用的所有参考文献、专利申请、专利、公开的专利申请的全部内容特此引用作为参考。
实施例
实施例1：谷氨酸棒状杆菌ATCC 13032的总基因组DNA的制备
谷氨酸棒状杆菌(ATCC 13032)的培养物在30℃于BHI培养基(Difco)中剧烈振摇过夜。离心收获细胞，弃上清并将细胞重悬于5ml缓冲液I(培养物原初体积的5％-所有显示的体积为按100ml培养物体积计算所得)。缓冲液I的组成：140.34g/l蔗糖，2.46g/l MgSO4·7H2O，10ml/l KH2PO4溶液(100g/l，用KOH调节至pH6.7)，50ml/l M12浓缩物(10g/l(NH4)2SO4，1g/lNaCl，2g/l MgSO4·7H2O，0.2g/l CaCl2，0.5g/l酵母提取物(Difco)，10ml/l痕量元素混合物(200mg/l FeSO4·H2O，10mg/l ZnSO4·7H2O，3mg/lMnCl2·4H2O，30mg/l H3BO3，20mg/l CoCl2·6H2O，1mg/l NiCl2·6H2O，3mg/l Na2MoO4·2H2O)，500mg/l络合剂(EDTA或柠檬酸)，100ml/l维生素混合物(0.2mg/l生物素，0.2mg/l叶酸，20mg/l对氨基苯甲酸，20mg/l核黄素，40mg/l泛酸钙，140mg/l烟酸，40mg/l维生素B6盐酸盐，200mg/l肌醇)。向悬液中加入溶菌酶至终浓度2.5mg/ml。在37℃温育约4小时后，细胞壁降解，并离心收获所得的原生质体。沉淀用5ml缓冲液I洗一次并用5ml TE缓冲液(10mM Tris-HCl，1mM EDTA，pH值8)洗一次。将沉淀重悬于4mlTE缓冲液，并加入0.5ml SDS溶液(10％)和0.5ml NaCl溶液(5M)。加入蛋白酶K至终浓度200μg/ml后，将悬液在37℃温育约18小时。使用标准方法用酚、酚/氯仿/异戊醇和氯仿/异戊醇提取而纯化DNA。然后，加入1/50体积的3M醋酸钠和2体积的乙醇，于-20℃温育30分钟，并在高速离心机上使用SS34转子(Sorvall)于12,000rpm离心30分钟，沉淀DNA。将该DNA溶于含20μg/ml RNaseA的1ml TE缓冲液中，并用1000ml TE缓冲液于4℃透析至少3小时。在此期间，更换缓冲液3次。向分装的0.4ml透析过的等分DNA溶液中加入0.4ml 2M LiCl和0.8ml乙醇。于-20℃温育30分钟后，离心(13,000rpm，Biofuge Fresco，Heraeus，Hanau，德国)收集DNA。将该DNA沉淀溶于TE缓冲液。用此方法制备的DNA可用于所有目的，包括Southern印迹和构建基因组文库。
实施例2：在大肠杆菌中构建谷氨酸棒状杆菌(ATCC13032)的基因组文库
使用如实施例1所述制备的DNA，按照公知的且已建立的方法(参见例如，Sambrook，J.等人(1989)“分子克隆：实验室手册”，冷泉港实验室出版社，或Ausubel，F.M.等人(1994)“分子生物学最新方法”，John Wiley &Sons.)构建粘粒和质粒文库。
可以使用任何质粒或粘粒。特别优选使用的是质粒pBR322(Sutcliffe，J.G.(1979)美国国家科学院院报(Proc.Natl.Acad.Sci.USA)75：3737-3741)、pACYC177(Change和Cohen(1978)细菌学杂志(J.Bacteriol)134：1141-1156)、pBS系列的质粒(pBSSK+，pBSSK-及其它；Stratagene，LaJolla，USA)、或粘粒如SuperCos1(Stratagene，LaJolla，美国)或Lorist6(Gibson，T.J.，Rosenthal A.和Waterson，R.H.(1987)基因(Gene)53：283-286。
实施例3：DNA序列测定和计算机功能分析
如实施例2所述的基因组文库按照标准方法用于DNA序列测定，尤其是使用ABI377序列测定仪通过链终止法测定(参见例如，Fleischman，R.D.等人(1995)“流感嗜血菌(Haemophilus Influenzae)Rd的全基因组随机序列测定和组装”，科学(Science)，269：496-512)。使用具有以下核苷酸序列的测序引物：5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。
实施例4：体内诱变
谷氨酸棒状杆菌的体内诱变可通过将质粒(或其它载体)DNA在不能维持其遗传信息完整性的大肠杆菌或其它微生物(例如芽孢杆菌属种或酵母如酿酒酵母)中传代而实施。通常的增变菌株在用于DNA修复系统的基因中有突变(例如mutHLS、mutD、mutT等，对照参见Rupp，W.D.(1996)，《大肠杆菌和沙门氏菌》，ASM：Washington一书中的DNA修复机制，2277-2294页)。这些菌株为技术人员熟知。这些菌株的用途在例如Greener，A.和Callahan，M.(1994)Strategies 7：32-34中进行了阐述。
实施例5：在大肠杆菌和谷氨酸棒状杆菌之间的DNA转移
几种棒状杆菌和短杆菌物种含有自主复制的内源质粒(例如pHM1519或pBL1)(综述参见例如，Martin，J.F.等人(1987)生物技术(Biotechnology)，5：137-146)。使用标准的大肠杆菌载体可以容易地构建用于大肠杆菌和谷氨酸棒状杆菌的穿梭载体(Sambrook，J.等人(1989)，“分子克隆：实验室手册”，冷泉港实验室出版社，或Ausubel，F.M.等人(1994)“分子生物学最新方法”(”Current Protocols in Molecular Biology”)，John Wiley & Sons)，其中向大肠杆菌载体加入用于谷氨酸棒状杆菌中的复制起点和来自谷氨酸棒状杆菌的适当标记。此类复制起点优选取自从棒状杆菌和短杆菌分离的内源质粒。对于这些物种，作为转化标记尤其有用的是卡那霉素抗性基因(如源自Tn5或Tn903转座子的基因)或氯霉素抗性基因(Winnacker，E.L.(1987)“从基因到克隆-基因技术简介”(“From Genes to Clones-Introduction to Gene Technology”)，VCH，Weinheim)。在文献中有许多在大肠杆菌和谷氨酸棒状杆菌中复制的多种穿梭载体的构建例子，这些载体可用于多种目的，包括基因过表达(参见例如，Yoshihama，M.等人(1985)细菌学杂志(J.Bacteriol.)162：591-597，Martin J.F.等人(1987)生物技术(Biotechnology)，5：137-146和Eikmanns，B.J.等人(1991)基因(Gene)，102：93-98)。
使用标准技术，可将目的基因克隆入上述的穿梭载体之一中并可将此杂合载体导入谷氨酸棒状杆菌菌株。谷氨酸棒状杆菌的转化可通过原生质体转化(Kastsumata，R.等人(1984)细菌学杂志(J.Bacteriol.)159，306-311)、电穿孔(Liebl，E.等人(1989)FEMS Microbiol.Letters，53：399-303)以及当使用特别的载体时，也可通过接合(如在Sch_fer，A等人(1990)细菌学杂志(J.Bacteriol.)172：1663-1666中所述)来完成。同样也可通过制备谷氨酸棒状杆菌的质粒DNA(使用本领域熟知的标准方法)并将其转化至大肠杆菌，将谷氨酸棒状杆菌的穿梭载体转移至大肠杆菌中。该转化步骤可使用标准方法进行，但有利的是使用Mcr-缺陷的大肠杆菌菌株，如NM522(Gough和Murray(1983)分子生物学杂志(J.Mol.Biol.)166：1-19)。
实施例6：测定突变蛋白的表达
在转化的宿主细胞中对突变蛋白活性的观察依赖于突变蛋白与野生型蛋白以相似的方式和相似的量表达的事实。确定突变基因的转录的量(指示可用于翻译为基因产物的mRNA量)的适宜方法是进行Northern印迹(参考文献见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocolsin Molecular Biology)，Wiley：纽约)，其中设计为与目的基因结合的引物用可检测标志(通常为放射性的或化学发光的)标记，这样当提取生物体培养物的总RNA、在凝胶上分级分离、转移至稳定的基质上并与该探针温育后，探针的结合和结合量将指示该基因的mRNA的存在和存在量。该信息指示了突变基因的转录程度。通过多种本领域公知的方法可从谷氨酸棒状杆菌中制备总细胞RNA，如在Bormann，E.R.等人(1992)分子微生物学(Mol.Microbiol.)6：317-326中所描述的。
从所述mRNA翻译的蛋白的存在或相对量，可使用标准技术如Western印迹(参见例如，Ausubel等人(1988)分子生物学最新方法(Current Protocols in Molecular Biology)，Wiley：New York)测定。在该方法中，提取细胞总蛋白、通过凝胶电泳分级分离、转移至基质如硝化纤维素上并与能和目的蛋白特异性结合的探针如抗体一起温育。该探针通常用易于检测到的化学发光或显色标记物标记。所观察到的标记物的存在和量将指示在细胞中目的突变蛋白的存在和量。
实施例7：遗传改变的谷氨酸棒状杆菌的生长—培养基和培养条件
遗传修饰的棒状杆菌培养在合成或天然的生长培养基中。用于棒状杆菌的许多种不同生长培养基为人们熟知并易于获得(Lieb等人(1989)Appl.Microbiol.Biotechnol.，32：205-210；yon der Osten等人(1998)Biotechnology Letters，11：11-16；专利DE 4,120,867；在《原核生物》(TheProcaryotes)，Volume II，Balows，A.等人编辑.Springer-Verlag一书中的Liebl(1992)“棒状杆菌属”(“The Genus Corynebacteria”))。这些培养基由一种或多种碳源、氮源、无机盐、维生素和痕量元素组成。优选的碳源是糖类，如单糖、二糖或多糖。非常好的碳源的实例是葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉和纤维素。也可通过复杂化合物如糖蜜或其它来自糖加工的副产品向培养基中提供糖。加入不同碳源的混合物也可能是有利的。其它可能的碳源为醇类和有机酸，如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物，或为包含这些化合物的物质。示例性氮源包括氨气和胺盐如NH4Cl或(NH4)2SO4、NH4OH、硝酸盐、尿素、氨基酸和复杂氮源如玉米浆、大豆粉、大豆蛋白、酵母提取物、肉膏等。
可包括在培养基中的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的氯化物、磷盐或硫酸盐。可向培养基中加入螯合剂以维持溶液中的金属离子。尤其适宜的螯合剂包括二羟基酚，如儿茶酚或原儿茶酸盐，和有机酸如柠檬酸。培养基一般也包括其它生长因子如维生素或生长促进剂，其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸盐和维生素B6。生长因子和盐常来自复杂的培养基组分，如酵母提取物、糖蜜、玉米浆等。培养基的确切组成强烈取决于具体实验并在每一具体情况下单独确定。有关培养基优化的信息在教科书“Applied Microbiol.Physiology，A Practical Approach”(P.M.Rhodes，P.F.Stanbury编辑，IRL Press(1997)53-73页，ISBN 0 19 963577 3)中可找到。也可从供应商处得到生长培养基，如Standard 1(Merck)或BHI(Brain heart infusion，DIFCO)等。
通过加热(1.5巴，121℃加热20分钟)或过滤除菌对所有的培养基组成成份进行灭菌。组成成份可一起灭菌，或需要的话分开灭菌。所有的培养基组成成份可在培养开始时即存在，或它们可按需要连续加入或分批加入。
对每一实验分别确定培养条件。温度应在15℃和45℃的范围之间。在实验中温度可保持恒定或可改变。培养基的pH应在5至8.5的范围之间，优选地在7.0附近，可通过向培养基中添加缓冲液而维持。用于此目的的示例性缓冲液为磷酸钾缓冲液。可备选地或同时地使用合成的缓冲液如MOPS、HEPES、ACES和其它。在生长过程中通过加入NaOH或NH4OH也可维持恒定的培养pH。如果使用复杂的培养基组分如酵母提取物，可减轻对额外的缓冲液的需要，因为许多复杂化合物具有高缓冲容量。如果使用发酵罐培养微生物，用气态氨也可调节pH。
培养时间通常在数小时到数天之间。选择培养时间以允许肉汤中累积最大量的产物。可在多种容器如不同规格的微滴定板、玻璃管、玻璃烧瓶或玻璃或金属发酵罐中实施所公开的生长实验。对于筛选大量的克隆，应在微滴定板、玻璃管或在有或没有挡板的摇瓶中培养微生物。优选地使用100ml摇瓶，其中装有10％(体积)的所需生长培养基。该烧瓶应在旋转振荡器(振幅25mm)上以100-300rpm的速度摇动。可通过维持潮湿空气减少蒸发损失；备选地，对蒸发损失应进行数学上的校正。
如果研究经遗传修饰后的克隆，也应分析未修饰的对照克隆或含有无任何插入的基本质粒的对照克隆。使用已于30℃温育的在琼脂板如CM板(10g/l葡萄糖，2.5g/l NaCl，2g/l尿素，10g/l聚胨，5g/l酵母抽取物，5g/l肉膏，22g/l琼脂，用2M NaOH调节至pH6.8)上生长的细胞接种培养基至OD600 0.5-1.5。培养基的接种通过导入来自CM板的谷氨酸棒状杆菌细胞的盐水溶液或添加所述细菌的液体预培养物而完成。
实施例8：突变蛋白功能的体外分析
酶活性和动力学参数的确定是本领域熟知的。为了确定特定的修饰的酶的活性，实验应加以改良以适应野生型酶的比活性，这在本领域普通技术人员的能力范围内。例如可在下列参考文献中找到对酶的一般性总结以及有关结构、动力学、原理、方法、应用和多种酶活性的测定例子的具体细节：Dixon，M.和Webb，E.C.，(1979)酶(Enzymes)，Longmans，伦敦；Fersht，(1985)酶结构和机制(Enzyme Structure and Mechanism)，Freeman，纽约；Walsh，(1979)酶促反应机制(Enzymatic ReactionMechanisms)，Freeman，旧金山；Price，N.C.，Stevens，L.(1982)酶学基础(Fundamentals of Enzymology)，牛津大学出版社：Oxford；Boyer，P.D.编辑(1983)酶(The Enzymes)，第三版，Academic Press：纽约；Bisswanger，H.，(1994)酶动力学(Enzymkinetik)，第二版.VCH：Weinheim(ISBN3527300325)；Bergmeyer，H.U.，Bergmeyer，J.，Graβl，M.编辑(1983-1986)酶分析方法(Methods of Enzymatic Analysis)，第三版，I-XII卷，VerlagChemie：Weinheim；以及Ullmann工业化学百科全书(Ullmann’sEncyclopedia of Industrial Chemistry)(1987)A9卷，”酶”.VCH：Weinheim，352-363页。
可通过几种已建立的方法测定结合DNA的蛋白的活性，如DNA条带移位分析(也称为凝胶阻滞试验)。这些蛋白对其它分子表达的作用可使用报告基因试验测定(如在Kolmar，H.等人(1995)EMBO J.14：3895-3904及其中所引用的参考文献中所述)。报告基因测试系统为人们所熟知，并被建立以应用于原核和真核细胞，其中使用酶如β-半乳糖苷酶、绿色荧光蛋白和其它几种酶。
可根据如在《生物膜，分子结构和功能》(Biomembranes，MolecularStructure and Function)，Springer：Heidelberg一书中的Gennis，R.B.(1989)”孔、通道和转运蛋白”，85-137；199-234和270-322页中所述的技术进行膜转运蛋白活性的测定。
实施例9：分析突变蛋白对目的产物产生的影响
谷氨酸棒状杆菌中的遗传修饰对目的化合物(如氨基酸)产生的影响可通过如下方式确定：将经修饰的微生物在合适的条件(如上文中所描述的那些条件)下生长，并针对目的产物(即氨基酸)产生的增加来分析培养基和/或细胞成分。此类分析技术为本领域普通技术人员熟知，包括光谱学、薄层色谱法、多种显色法、酶学和微生物学方法、以及分析色谱法如高效液相色谱法(参见例如，Ullman工业化学百科全书(Ullman，Encyclopediaof Industrial Chemistry)，A2卷，89-90和443-613页，VCH：Weinheim(1985)；Fallon，A.等人(1987)《生物化学和分子生物学实验室技术》(LaboratoryTechniques in Biochemistry and Molecular Biology)中的“HPLC在生物化学中的应用”，17卷；Rehm等人(1993)生物技术(Biotechnology)，3卷，III章“产物回收和纯化”，469-714页，VCH：Weinheim；Belter，P.A.等人(1988)生物分离：生物技术中的下游加工(Bioseparations：downstreamprocessing for Biotechnotogy)，John Wiley and Sons；Kennedy，J.F.和Cabral，J.M.S.(1992)生物物质的回收方法(Recovery processes forbiological materials)，John Wiley and Sons；在《Ulmann工业化学百科全书》一书中的Shaeiwitz，J.A.和Henry，J.D.(1988)生物化学分离，B3卷，11章，1-27页，VCH：Weinheim；以及Dechow，F.J.(1989)生物技术中的分离和纯化技术(Separation and purification techniques in biotechnology)，Noyes Publications.)。
除了测定发酵终产物外，同样还可分析用于产生目的化合物的代谢途径的其它成分如中间体和副产物来确定化合物的总体生产效率。分析方法包括测定培养基中的营养素水平(例如，糖类、碳氢化合物、氮源、磷酸盐和其它离子)、测定生物量组成和生长、分析生物合成途径中共同代谢物的产生及测定发酵过程中产生的气体。这些测定的标准方法在《应用微生物生理学，实用方法》(Applied Microbial Physiology，A Practical Approach)，P.M.Rhodes和P.F.Stanbury编辑，IRL Press，103-129；131-163和165-192页(ISBN：0199635773)及其引用的参考文献中描述。
实施例10：从谷氨酸棒状杆菌培养物中纯化目的产物
可通过多种本领域熟知的方法从谷氨酸棒状杆菌细胞或上述培养物上清回收目的产物。如果目的产物不从细胞向外分泌，可低速离心从培养物中收获细胞、用标准技术如机械力或超声裂解细胞。离心去除细胞碎片，获得含有可溶蛋白的上清级分用于进一步纯化目的化合物。如果产物从谷氨酸棒状杆菌细胞分泌，则低速离心从培养物中去除细胞，并保留上清级分用于进一步纯化。
将来自任一种纯化方法的上清级分用合适的树脂进行层析，其中目的分子保留在层析树脂上而样本中的许多杂质不保留在层析树脂上，或者杂质保留在层析树脂上而样本不保留。此层析步骤必要时可使用相同或不同的层析树脂重复。本领域普通技术人员在选择合适的层析树脂以及将它们最有效地用于特定的待纯化分子方面是很精通的。可通过过滤或超滤浓缩纯化产物，并贮存在产物稳定性最高的温度下。
本领域公知许多纯化方法，它们不限于上文的纯化方法，并且在如Bailey，J.E.和Ollis，D.F.生物化学工程基础(Biochemical EngineeringFundamentals)，McGraw-Hill：纽约(1986)中描述。
分离的化合物的身份和纯度可通过本领域的标准技术确定。这些技术包括高效液相色谱(HPLC)、光谱法、显色法、薄层色谱法、NIRS、酶学试验或微生物学方法。此类分析方法编纂于：Patek等人(1994)Appl.Environ.Microbiol.60：133-140；Malakhova等人(1996)生物技术(Biotekhnologiya)11：27-32；和Schmidt等人(1998)生物加工工程(Bioprocess Engineer)19：67-70；Ulmann工业化学百科全书(Ulmann’s Encyclopedia of Industrial Chemistry)(1996)A27卷，VCH：Weinheim，89-90页，521-540页，540-547页，559-566页，575-581页和581-587页；Michal，G.(1999)生物化学途径：生物化学和分子生物学图集(BiochemicalPathways：An Atlas of Biochemistry and Molecular Biology)，John Wileyand Sons；《生物化学和分子生物学中的实验室技术》17卷中的Fallon，A.等人(1987)HPLC在生物化学中的应用。
等价方案
技术人员知晓或者能够通过简单地使用常规的方法确定本发明具体实施方案的大量等价方案。这些等价方案旨在包括在下面的权利要求书中。
表1中信息的理解如下：
在第1列“DNA ID”中，每种情况下相关数字指所附序列表的SEQID NO。所以，“DNA ID”列中的“5”指SEQ ID NO：5。
在第2列“AA ID”中，每种情况下相关数字指所附序列表的SEQ IDNO。因此，“AA ID”列中的“6”指SEQ ID NO：6。
在第3列“标识”中，列出了每个序列的明确的内部名称。
在第4列“AA POS”中，每种情况下相关数字指相同行中多肽序列“AA ID”的氨基酸位置。所以，“AA pos”列中“26”是相应所示的多肽序列的氨基酸位置26。位置计算从N末端为+1开始。
在第5列“AA野生型”中，每种情况下相关字母指相应野生型菌株中位于第4列中所示的位置处的氨基酸(用单字母代码表示)。
在第6列“AA突变体”中，每种情况下相关字母指相应突变菌株中位于第4列中所示的位置处的氨基酸(用单字母代码表示)。
在第7列“功能”中，列出了相应多肽序列的生理学功能。
蛋白原性氨基酸的单字母代码：
A丙氨酸
C半胱氨酸
D天冬氨酸
E谷氨酸
F苯丙氨酸
G甘氨酸
H组氨酸
I异亮氨酸
K赖氨酸
L亮氨酸
M甲硫氨酸
N天冬酰胺
P脯氨酸
Q谷氨酰胺
R精氨酸
S丝氨酸
T苏氨酸
V缬氨酸
W色氨酸
Y酪氨酸
表1
编码DNA复制和病理蛋白的基因DNA ID：AA ID：标识AA POSAA野生型AA突变体功能：12RXA0005066AV依赖ATP的RNA解旋酶DEAD596GS依赖ATP的RNA解旋酶DEAD34RXA00061754SNDNA聚合酶I(EC 2.7.7.7)56RXA0015790VI浸染素(Invasin)1159GR浸染素(Invasin)1449GD浸染素(Invasin)1454SF浸染素(Invasin)1590SF浸染素(Invasin)178RXA0020845GEVULNIBACTIN利用蛋白(UTILIZATION PROTEIN) VIUB61DNVULNIBACTIN利用蛋白(UTILIZATION PROTEIN) VIUB76PLVULNIBACTIN利用蛋白(UTILIZATION PROTEIN) VIUB910RXA0031369EK23S RRNA甲基转移酶(EC 2.1.1.-)1112RXA00341254GD核糖核酸外切酶II(EC 3.1.13.1)414DG核糖核酸外切酶II(EC 3.1.13.1)1314RXA00407187DHDNA聚合酶IIIα链(EC 2.7.7.7)1516RXA0069434ATSSU核糖体蛋白S8P1718RXA0069666TILSU核糖体蛋白L18P1920RXA0080762GEDNA聚合酶III的δ亚基(EC 2.7.7.7)241RCDNA聚合酶III的δ亚基(EC 2.7.7.7)2122RXA01238156SNDNA聚合酶IIIα链(EC 2.7.7.7)2324RXA0128043KRSSU核糖体蛋白S12P2526RXA01305400LF粘附素AIDA-I545SF粘附素AIDA-I2728RXA0134311AVLSU核糖体蛋白L1P2930RXA01480297GDDNA引发酶(EC 2.7.7.-)3132RXA01563117PS核糖核酸酶D(EC 3.1.26.3)375RQ核糖核酸酶D(EC 3.1.26.3)3334RXA01581274SF23S RRNA甲基转移酶(EC 2.1.1.-)3536RXA0166164GDRRNA甲基转移酶(EC 2.1.1.-)3738RXA01683130RCDNA促旋酶亚基A(EC 5.99.1.3)584SFDNA促旋酶亚基A(EC 5.99.1.3)3940RXA01718284PSCBS结构域包含蛋白4142RXA01736236PS推测的依赖ATP的DNA解旋酶4344RXA01770243DNDNA解旋酶II(EC 3.6.1.-)4546RXA01772502SFDNA和RNA解旋酶超家庭II4748RXA0194997SNLSU核糖体蛋白L23P4950RXA0196646AV寡核糖核酸酶(EC 3.1.-.-)5152RXA02070150ATMRP蛋白同系物5354RXA02082726AV染色体分离蛋白SMC2781LF染色体分离蛋白SMC2905AT染色体分离蛋白SMC25556RXA02145321PL甲萘醌基(menaquinol)-细胞色素C还原酶细胞色素B亚单位5758RXA02293349AV依赖ATP的解旋酶HRPB5960RXA02357896GE可能是依赖ATP的解旋酶LHR(EC 3.6.1.-)1414TI可能是依赖ATP的解旋酶LHR(EC 3.6.1.-)6162RXA02363728VM依赖ATP的解旋酶6364RXA02533142RC转录调节蛋白6566RXA02657840HY可能是DNA聚合酶III的α链(EC 2.7.7.7)1161DN可能是DNA聚合酶III的α链(EC 2.7.7.7)6768RXA031661183PL依赖ATP的解旋酶HRPA6970RXA03266103TIDNA聚合酶III的β链(EC 2.7.7.7)364PLDNA聚合酶III的β链(EC 2.7.7.7)7172RXA0333252GE噬菌体Hau3抗性蛋白7374RXA03341153EKDNA结合蛋白7576RXA03590398PS毒力相关蛋白E7778RXA03607176RK推测为噬菌体蛋白7980RXA03743157SFDNA拓朴异构酶I(EC 5.99.1.2)8182RXA03903811EKDNA拓朴异构酶I(EC 5.99.1.2)8384RXA04194922RC依赖ATP的DNA解旋酶8586RXA07017574PSDNA聚合酶III的γ和τ亚单位(EC 2.7.7.7)8788RXA070181245LFDNA指导的RNA聚合酶β链(EC 2.7.7.6)8990RXA0701955GSLSU核糖体蛋白L2P9192RXA07020314RS苯丙氨酰-TRNA合成酶α链(EC 6.1.1.20)9394RXA07027256GD噬菌体侵染蛋白(PHAGE INFECTION PROTEIN)9596RXA0702827EKII型限制修饰系统限制亚单位310AVII型限制修饰系统限制亚单位
【序列表】
<110>巴斯福股份公司(BASF Aktiengesellschaft)
<120>编码DNA复制蛋白和病理相关蛋白的基因
<130>O.Z.0050/52977
<160>96
<210>1
<211>2326
<212>DNA
<213>谷氨酸棒状杆菌(Corynebacterium glutamicum)
<220>
<221>CDS
<222>(101)..(2296)
<223>RXA00050
<400>1
agcaatgtcc cattttccct gctcacctgt atgggcaccc gcggcggaag tggaattgca 60
tatggagttt tgatgatatt tagcgtaact taaaggaaca atg agt aat acc gag 115
Met Ser Asn Thr Glu
1 5
aac gtc aac ggc gac gta gaa cag ccg aat aac gtc att tcg tcg gaa 163
Asn Val Asn Gly Asp Val Glu Gln Pro Asn Asn Val Ile Ser Ser Glu
10 15 20
tct cag gaa acc ccg cag ggt gac tca gca tca gct gac ttc gct ctc 211
Ser Gln Glu Thr Pro Gln Gly Asp Ser Ala Ser Ala Asp Phe Ala Leu
25 30 35
gaa acc cca acc aac act gtt gaa gat gca cca gca tct gag ggt agc 259
Glu Thr Pro Thr Asn Thr Val Glu Asp Ala Pro Ala Ser Glu Gly Ser
40 45 50
gaa gag atc acc agg gtt gcg gat act tct gag gac gcc gac tct gca 307
Glu Glu Ile Thr Arg Val Ala Asp Thr Ser Glu Asp Ala Asp Ser Ala
55 60 65
gat gca gac aac gcg agc aat gta atc aat gag aat gag gac tcc tcg 355
Asp Ala Asp Asn Ala Ser Asn Val Ile Asn Glu Asn Glu Asp Ser Ser
70 75 80 85
gaa ggt gct aac cag cct tca aac gag tca tcc tct acg gaa gcc aaa 403
Glu Gly Ala Asn Gln Pro Ser Asn Glu Ser Ser Ser Thr Glu Ala Lys
90 95 100
tcc ggc ttc gat gca ctc gga ctg cca gag cgt gtt ctt gac gct gtg 451
Ser Gly Phe Asp Ala Leu Gly Leu Pro Glu Arg Val Leu Asp Ala Val
105 110 115
cgc aag gtg ggt tac gaa act cct tcc cca att cag gca caa acc atc 499
Arg Lys Val Gly Tyr Glu Thr Pro Ser Pro Ile Gln Ala Gln Thr Ile
120 125 130
cca atc ctc atg gag ggc cag gat gtt gtt ggt cta gca cag acc ggt 547
Pro Ile Leu Met Glu Gly Gln Asp Val Val Gly Leu Ala Gln Thr Gly
135 140 145
acc ggt aag act gca gct ttc gcg ctg cca atc ctt gcc cgt att gac 595
Thr Gly Lys Thr Ala Ala Phe Ala Leu Pro Ile Leu Ala Arg Ile Asp
150 155 160 165
aag tcc gtg cgc agc cca cag gca ctt gtg ctt gcc cct acc cgt gag 643
Lys Ser Val Arg Ser Pro Gln Ala Leu Val Leu Ala Pro Thr Arg Glu
170 175 180
ctg gca ctt cag gtt gct gac tcc ttc caa tcc ttc gct gac cac gtc 691
Leu Ala Leu Gln Val Ala Asp Ser Phe Gln Ser Phe Ala Asp His Val
185 190 195
ggt ggc ctg aac gtt ctg cca atc tat ggt gga cag gct tac ggc att 739
Gly Gly Leu Asn Val Leu Pro Ile Tyr Gly Gly Gln Ala Tyr Gly Ile
200 205 210
cag ctc tct ggc ctg cgt cgt ggc gct cac atc gtc gtg ggt acc cca 787
Gln Leu Ser Gly Leu Arg Arg Gly Ala His Ile Val Val Gly Thr Pro
215 220 225
ggc cga atc atc gat cac ctc gaa aag ggc tcc ctg gat atc tcc gga 835
Gly Arg Ile Ile Asp His Leu Glu Lys Gly Ser Leu Asp Ile Ser Gly
230 235 240 245
ctg cgc ttc ctc gtg ctc gat gaa gca gac gag atg ctg aac atg ggc 883
Leu Arg Phe Leu Val Leu Asp Glu Ala Asp Glu Met Leu Asn Met Gly
250 255 260
ttc cag gaa gat gtc gag cgc atc ctc gag gac acc cca gac gag aag 931
Phe Gln Glu Asp Val Glu Arg Ile Leu Glu Asp Thr Pro Asp Glu Lys
265 270 275
cag gtt gca cta ttc tcc gca acg atg cca aac ggc att cgt cgc ctg 979
Gln Val Ala Leu Phe Ser Ala Thr Met Pro Asn Gly Ile Arg Arg Leu
280 285 290
tcc aag cag tac ctg aac aac cct gct gaa atc acc gtt aag tcc gag 1027
Ser Lys Gln Tyr Leu Asn Asn Pro Ala Glu Ile Thr Val Lys Ser Glu
295 300 305
acc agg act aac acc aac atc acc cag cgc ttc ctc aac gtt gca cac 1075
Thr Arg Thr Asn Thr Asn Ile Thr Gln Arg Phe Leu Asn Val Ala His
310 315 320 325
cgc aac aag atg gat gca ctg acc cgt att ctc gag gtc acc gag ttt 1123
Arg Asn Lys Met Asp Ala Leu Thr Arg Ile Leu Glu Val Thr Glu Phe
330 335 340
gae gca atg atc atg ttc gtg cgc acc aag cac gaa act gaa gaa gtt 1171
Glu Ala Met Ile Met Phe Val Arg Thr Lys His Glu Thr Glu Glu Val
345 350 355
gct gaa aag ctc cgt gca cgc gga ttc tcc gca gca gcc atc aac ggc 1219
Ala Glu Lys Leu Arg Ala Arg Gly Phe Ser Ala Ala Ala Ile Asn Gly
360 365 370
gac att gct cag gca cag cgt gag cgc acc gtc gac cag ctg aag gac 1267
Asp Ile Ala Gln Ala Gln Arg Glu Arg Thr Val Asp Gln Leu Lys Asp
375 380 385
ggc cgc ctg gac atc ctc gtt gca acc gac gtt gca gcc cgt ggt ctt 1315
Gly Arg Leu Asp Ile Leu Val Ala Thr Asp Val Ala Ala Arg Gly Leu
390 395 400 405
gac gtt gag cgc atc tcc cac gtg ctt aac ttc gac att cca aac gac 1363
Asp Val Glu Arg Ile Ser His Val Leu Asn Phe Asp Ile Pro Asn Asp
410 415 420
acc gag tcc tac gtt cac cgc atc ggc cgc acc ggc cgt gca gga cgt 1411
Thr Glu Ser Tyr Val His Arg Ile Gly Arg Thr Gly Arg Ala Gly Arg
425 430 435
acc ggc gag gca atc ctg ttc gtg acc cca cgt gag cgt cgt atg ctt 1459
Thr Gly Glu Ala Ile Leu Phe Val Thr Pro Arg Glu Arg Arg Met Leu
440 445 450
cgc tcc atc gag cgc gca acc aac gca cca ctg cac gaa atg gaa ctg 1507
Arg Ser Ile Glu Arg Ala Thr Asn Ala pro Leu His Glu Met Glu Leu
455 460 465
cca acc gtc gat cag gtc aac gac ttc cgc aag gtc aag ttc gct gac 1555
Pro Thr Val Asp Gln Val Asn Asp Phe Arg Lys Val Lys Phe Ala Asp
470 475 480 485
tcc atc acc aag tcc ctc gag gac aag cag atg gac ctg ttc cgc acc 1603
Ser Ile Thr Lys Ser Leu Glu Asp Lys Gln Met Asp Leu Phe Arg Thr
490 495 500
ctg gtc aag gaa tac tcc cag gcc aac gac gtt cct cta gag gac atc 1651
Leu Val Lys Glu Tyr Ser Gln Ala Asn Asp Val Pro Leu Glu Asp Ile
505 510 515
gca gcg gca ctg gca acc cag gca cag tcc ggc gac ttc ctg ctc aag 1699
Ala Ala Ala Leu Ala Thr Gln Ala Gln Ser Gly Asp Phe Leu Leu Lys
520 525 530
gag ctc cca cca gag cgc cgt gag cgc aac gac cgc cgt cgt gac cgt 1747
Glu Leu Pro Pro Glu Arg Arg Glu Arg Asn Asp Arg Arg Arg Asp Arg
535 540 545
gac ttc gac gac cgt ggt gga cgt gga cgc gac cgt gac cgt ggc gac 1795
Asp Phe Asp Asp Arg Gly Gly Arg Gly Arg Asp Arg Asp Arg Gly Asp
550 555 560 565
cgc gga gat cgt ggc tca cgc ttc gac cgc gac gac gag aac ctg gca 1843
Arg Gly Asp Arg Gly Ser Arg Phe Asp Arg Asp Asp Glu Asn Leu Ala
570 575 580
acc tac cgc ctc gca gtg ggc aag cgc cag cac atc cgc cca ggc gca 1891
Thr Tyr Arg Leu Ala Val Gly Lys Arg Gln His Ile Arg Pro Gly Ala
585 590 595
atc gtt ggt gca ctt gcc aac gaa ggt ggc ctg aac tcc aag gac ttc 1939
Ile Val Gly Ala Leu Ala Asn Glu Gly Gly Leu Asn Ser Lys Asp Phe
600 605 610
ggc cgc atc acc atc gca gcc gac cac acc ctg gtt gaa ctg cca aag 1987
Gly Arg Ile Thr Ile Ala Ala Asp His Thr Leu Val Glu Leu Pro Lys
615 620 625
gat ctc cca cag agc gtt ctt gac aac ctg cgc gac acc cgc atc tcc 2035
Asp Leu Pro Gln Ser Val Leu Asp Asn Leu Arg Asp Thr Arg Ile Ser
630 635 640 645
ggc cag ctc atc aac ata gaa cgc gac tcc ggt gga cgc cca cca cgc 2083
Gly Gln Leu Ile Asn Ile Glu Arg Asp Ser Gly Gly Arg Pro Pro Arg
650 655 660
cgc ttc gag cgc gat gac cgt ggc gga cgc ggc gga ttc cgc ggc gac 2131
Arg Phe Glu Arg Asp Asp Arg Gly Gly Arg Gly Gly Phe Arg Gly Asp
665 670 675
cgt gat gac cgc ggt gga cgt gga cgt gac cgt gac gat cgt gga agc 2179
Arg Asp Asp Arg Gly Gly Arg Gly Arg Asp Arg Asp Asp Arg Gly Ser
680 685 690
cgt gga ggt ttc cgc ggt gga cgt gac cgt gat gat cgt ggc gga cgc 2227
Arg Gly Gly Phe Arg Gly Gly Arg Asp Arg Asp Asp Arg Gly Gly Arg
695 700 705
ggt gga ttc cgc gga cgc gac gac cgc gga gac cgt ggt ggc cgt ggc 2275
Gly Gly Phe Arg Gly Arg Asp Asp Arg Gly Asp Arg Gly Gly Arg Gly
710 715 720 725
ggt tac cgt ggc gga cgc gac taagagttcg ttttagcttc agctcaggtt 2326
Gly Tyr Arg Gly Gly Arg Asp
730
<210>2
<211>732
<212>PRT
<213>谷氨酸棒状杆菌
<400>2
Met Ser Asn Thr Glu Asn Val Asn Gly Asp Val Glu Gln Pro Asn Asn
1 5 10 15
Val Ile Ser Ser Glu Ser Gln Glu Thr Pro Gln Gly Asp Ser Ala Ser
20 25 30
Ala Asp Phe Ala Leu Glu Thr Pro Thr Asn Thr Val Glu Asp Ala Pro
35 40 45
Ala Ser Glu Gly Ser Glu Glu Ile Thr Arg Val Ala Asp Thr Ser Glu
50 55 60
Asp Ala Asp Ser Ala Asp Ala Asp Asn Ala Ser Asn Val Ile Asn Glu
65 70 75 80
Asn Glu Asp Ser Ser Glu Gly Ala Asn Gln Pro Ser Asn Glu Ser Ser
85 90 95
Ser Thr Glu Ala Lys Ser Gly Phe Asp Ala Leu Gly Leu Pro Glu Arg
100 105 110
Val Leu Asp Ala Val Arg Lys Val Gly Tyr Glu Thr Pro Ser Pro Ile
115 120 125
Gln Ala Gln Thr Ile Pro Ile Leu Met Glu Gly Gln Asp Val Val Gly
130 135 140
Leu Ala Gln Thr Gly Thr Gly Lys Thr Ala Ala Phe Ala Leu Pro Ile
145 150 155 160
Leu Ala Arg Ile Asp Lys Ser Val Arg Ser Pro Gln Ala Leu Val Leu
165 170 175
Ala Pro Thr Arg Glu Leu Ala Leu Gln Val Ala Asp Ser Phe Gln Ser
180 185 190
Phe Ala Asp His Val Gly Gly Leu Asn Val Leu Pro Ile Tyr Gly Gly
195 200 205
Gln Ala Tyr Gly Ile Gln Leu Ser Gly Leu Arg Arg Gly Ala His Ile
210 215 220
Val Val Gly Thr Pro Gly Arg Ile Ile Asp His Leu Glu Lys Gly Ser
225 230 235 240
Leu Asp Ile Ser Gly Leu Arg Phe Leu Val Leu Asp Glu Ala Asp Glu
245 250 255
Met Leu Asn Met Gly Phe Gln Glu Asp Val Glu Arg Ile Leu Glu Asp
260 265 270
Thr Pro Asp Glu Lys Gln Val Ala Leu Phe Ser Ala Thr Met Pro Asn
275 280 285
Gly Ile Arg Arg Leu Ser Lys Gln Tyr Leu Asn Asn Pro Ala Glu Ile
290 295 300
Thr Val Lys Ser Glu Thr Arg Thr Asn Thr Asn Ile Thr Gln Arg Phe
305 310 315 320
Leu Asn Val Ala His Arg Asn Lys Met Asp Ala Leu Thr Arg Ile Leu
325 330 335
Glu Val Thr Glu Phe Glu Ala Met Ile Met Phe Val Arg Thr Lys His
340 345 350
Glu Thr Glu Glu Val Ala Glu Lys Leu Arg Ala Arg Gly Phe Ser Ala
355 360 365
Ala Ala Ile Asn Gly Asp Ile Ala Gln Ala Gln Arg Glu Arg Thr Val
370 375 380
Asp Gln Leu Lys Asp Gly Arg Leu Asp Ile Leu Val Ala Thr Asp Val
385 390 395 400
Ala Ala Arg Gly Leu Asp Val Glu Arg Ile Ser His Val Leu Asn Phe
405 410 415
Asp Ile Pro Asn Asp Thr Glu Ser Tyr Val His Arg Ile Gly Arg Thr
420 425 430
Gly Arg Ala Gly Arg Thr Gly Glu Ala Ile Leu Phe Val Thr Pro Arg
435 440 445
Glu Arg Arg Met Leu Arg Ser Ile Glu Arg Ala Thr Asn Ala Pro Leu
450 455 460
His Glu Met Glu Leu Pro Thr Val Asp Gln Val Asn Asp Phe Arg Lys
465 470 475 480
Val Lys Phe Ala Asp Ser Ile Thr Lys Ser Leu Glu Asp Lys Gln Met
485 490 495
Asp Leu Phe Arg Thr Leu Val Lys Glu Tyr Ser Gln Ala Asn Asp Val
500 505 510
Pro Leu Glu Asp Ile Ala Ala Ala Leu Ala Thr Gln Ala Gln Ser Gly
515 520 525
Asp Phe Leu Leu Lys Glu Leu Pro Pro Glu Arg Arg Glu Arg Asn Asp
530 535 540
Arg Arg Arg Asp Arg Asp Phe Asp Asp Arg Gly Gly Arg Gly Arg Asp
545 550 555 560
Arg Asp Arg Gly Asp Arg Gly Asp Arg Gly Ser Arg Phe Asp Arg Asp
565 570 575
Asp Glu Asn Leu Ala Thr Tyr Arg Leu Ala Val Gly Lys Arg Gln His
580 585 590
Ile Arg Pro Gly Ala Ile Val Gly Ala Leu Ala Asn Glu Gly Gly Leu
595 600 605
Asn Ser Lys Asp Phe Gly Arg Ile Thr Ile Ala Ala Asp His Thr Leu
610 615 620
Val Glu Leu Pro Lys Asp Leu Pro Gln Ser Val Leu Asp Asn Leu Arg
625 630 635 640
Asp Thr Arg Ile Ser Gly Gln Leu Ile Asn Ile Glu Arg Asp Ser Gly
645 650 655
Gly Arg Pro Pro Arg Arg Phe Glu Arg Asp Asp Arg Gly Gly Arg Gly
660 665 670
Gly Phe Arg Gly Asp Arg Asp Asp Arg Gly Gly Arg Gly Arg Asp Arg
675 680 685
Asp Asp Arg Gly Ser Arg Gly Gly Phe Arg Gly Gly Arg Asp Arg Asp
690 695 700
Asp Arg Gly Gly Arg Gly Gly Phe Arg Gly Arg Asp Asp Arg Gly Asp
705 710 715 720
Arg Gly Gly Arg Gly Gly Tyr Arg Gly Gly Arg Asp
725 730
<210>3
<211>2845
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2815)
<223>RXA00061
<400>3
aatcaattgc agaactaacc cggttgtttc cgagccagtc tgaatgactg aaagcaatat 60
tagaccatca atgattagga atggaaatta ggggtctggt ttg ggt gaa tgt gtc 115
Leu Gly Glu Cys Val
1 5
gct aat ttt tcc act cgc cta cac tcg gga ggc gtg act gag aag act 163
Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly Val Thr Glu Lys Thr
10 15 20
gac cag acc tta atg ctt atc gac ggc cac tcg atg gct ttc cgc gca 211
Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser Met Ala Phe Arg Ala
25 30 35
ttc ttt gct ttg ccg gct gag aat ttc tcc acg tcg ggc ggg cag gcc 259
Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr Ser Gly Gly Gln Ala
40 45 50
acc aat gct gtc tat ggc ttt ctc tcg atg ctg tcc acg ttg ttg aag 307
Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu Ser Thr Leu Leu Lys
55 60 65
gat gag cag cct act cat gtg gcg gtg gct ttc gat gtg ggg cgt aag 355
Asp Glu Gln Pro Thr His Val Ala Val Ala Phe Asp Val Gly Arg Lys
70 75 80 85
acg ttc cgt acc gat atg ttc ccg gcg tat aag gcg cag cgt gaa gca 403
Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys Ala Gln Arg Glu Ala
90 95 100
acg cca cct gag ttt aag ggc cag gtg gaa atc ctc aag gag gtg ttg 451
Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile Leu Lys Glu Val Leu
105 110 115
tcc act ttg gga att acg act att gag aaa atc gat ttt gag gct gat 499
Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile Asp Phe Glu Ala Asp
120 125 130
gat gtg atc gcc acg ttg tct gtg gcg gcg aaa cct tta ggc ttt aag 547
Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys Pro Leu Gly Phe Lys
135 140 145
acg ctg att gtt acg ggt gac cgt gat tcc ttc cag ttg gtc aat gac 595
Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe Gln Leu Val Asn Asp
150 155 160 165
acc acc acg gtg ttg tat ccg atg aag ggc gtg tct gtg ctg cac cgt 643
Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val Ser Val Leu His Arg
170 175 180
ttc acg ccg gaa gca gtg gag gag aag tat gga ctg aca ccg agg cag 691
Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly Leu Thr Pro Arg Gln
185 190 195
tat ccg gag ttt gca gcg ctg cgt ggt gat cct tcc gat aac ttg cct 739
Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro Ser Asp Asn Leu Pro
200 205 210
aat att cct ggc gtg ggc gag aag act gct acc aag tgg att gcc cag 787
Asn Ile Pro Gly Val Gly Glu Lys Thr Ala Thr Lys Trp Ile Ala Gln
215 220 225
tat gaa act ttg gat aat ttg ctt gat cac gct gat gag atc aag ggc 835
Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala Asp Glu Ile Lys Gly
230 235 240 245
aag gtt ggc gcc agc ctg cgt gag cgc att gag cag gtc cgg atg aac 883
Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu Gln Val Arg Met Asn
250 255 260
cgc aag ctc acg gag atg gtg aag gat ctg gag ctg ccg ctt ggt ccg 931
Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu Leu Pro Leu Gly Pro
265 270 275
gac gat ttt gag atg aag cct gtg cag gtt gcg gag gtt gcg gcg aag 979
Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala Glu Val Ala Ala Lys
280 285 290
ttt gac gat ctg gag ttt ggt acc aat ttg cgt gag cgg gtg ctg gcg 1027
Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg Glu Arg Val Leu Ala
295 300 305
gtg gtg aag gcc gag ggt tcc gct gcc ccc gtg gag gaa gtg gaa gcg 1075
Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val Glu Glu Val Glu Ala
310 315 320 325
gaa cag gtt gtc gtc gat acg caa tct ttg gcg caa tgg ctg cct gct 1123
Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala Gln Trp Leu Pro Ala
330 335 340
agg gct ggc cag gcg ctt gct tta gcg ctg gct gga gtg gct aaa cct 1171
Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala Gly Val Ala Lys Pro
345 350 355
gct gct ggc gac acg tat gcg cta gcg att gcg gat acc aag cgc cat 1219
Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala Asp Thr Lys Arg His
360 365 370
gcg gtg ttg gtt gat gtg gct gat att tca gcg gag gat gaa aag gcg 1267
Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala Glu Asp Glu Lys Ala
375 380 385
ctg gcc acg tgg ttg gcg tcg gaa gat cca aag atg ctg cac ggc gct 1315
Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys Met Leu His Gly Ala
390 395 400 405
aag gcc gcc tat cat atg ctc gct ggg cgc ggt ttt gag ctg cac ggc 1363
Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly Phe Glu Leu His Gly
410 415 420
gtg gtg cat gac acg gcg atc gcg gca tac ttg ctg cgt ccg ggc caa 1411
Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu Leu Arg Pro Gly Gln
425 430 435
cgc acc tat gag ctt gcc gac gtc tac cag cgg cat ctt caa cga cag 1459
Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg His Leu Gln Arg Gln
440 445 450
ttg tct aca aac gac aat ggc ggc cag ctc acg ctg ctc gac gca gct 1507
Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr Leu Leu Asp Ala Ala
455 460 465
gat gac caa tcg ctt gtt gat gat gtc att gca atc ctt gag ctg tct 1555
Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala Ile Leu Glu Leu Ser
470 475 480 485
gaa gaa ttg acc aaa cag ctt cag gag att caa gct ttt gag ctt tac 1603
Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln Ala Phe Glu Leu Tyr
490 495 500
cat gac ctg gaa att ccg ctg tcg gga att ctg gcg cgc atg gag gcc 1651
His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu Ala Arg Met Glu Ala
505 510 515
atc ggt atc gct gtt gat gtt gcc act ttg gaa gag cag ttg aag act 1699
Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu Glu Gln Leu Lys Thr
520 525 530
ttc att ggt cag gtt gct cag gaa gag gaa gca gct cgc gag ctc gct 1747
Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala Ala Arg Glu Leu Ala
535 540 545
gag gat cca acc ctg aat ctc tcg agc ccg aag cag ctg caa gtg gtg 1795
Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys Gln Leu Gln Val Val
550 555 560 565
ctt ttt gag acg ttc gga atg ccg aaa acc aag aaa acc aag acc ggc 1843
Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys Lys Thr Lys Thr Gly
570 575 580
tac tct acg gct gcc gcg gaa att gaa gcc cta gcg atc aag aat ccg 1891
Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu Ala Ile Lys Asn Pro
585 590 595
cac cca ttc cta gat cac ctg ttg gca cac cgt cag tac caa aag atg 1939
His Pro Phe Leu Asp His Leu Leu Ala His Arg Gln Tyr Gln Lys Met
600 605 610
aag acc act ctg gaa ggt ctc atc cgt gag gtg gct cct gat ggc cgt 1987
Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val Ala Pro Asp Gly Arg
615 620 625
att cac acc acc ttc aac cag acg gtg gcg tct acg gga cgt ttg tca 2035
Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser Thr Gly Arg Leu Ser
630 635 640 645
tcc act gat ccc aac ctg caa aac att cct gtg cgc act gag gct ggc 2083
Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Glu Ala Gly
650 655 660
cga aag att cgt tcg gga ttc gtc gta ggc gag ggg tat gaa acc ttg 2131
Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu Gly Tyr Glu Thr Leu
665 670 675
ctg act gcc gac tat tcg cag att gaa atg cgc gtg atg gct cac ctt 279
Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg Val Met Ala His Leu
680 685 690
tcc cag gac cca ggc ttg att gag gcg tac cgc gaa ggc gaa gac ctg 2227
Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg Glu Gly Glu Asp Leu
695 700 705
cac aat tac gtg ggt tcc aag gtg ttt aat gtg ccc atc gat ggc gtg 2275
His Asn Tyr Val Gly Ser Lys Val Phe Asn Val Pro Ile Asp Gly Val
710 715 720 725
acc cct gag ctg cgt cgc cag gtc aag gcc atg tct tac ggt ctg gtg 2323
Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met Ser Tyr Gly Leu Val
730 735 740
tac ggc ttg tcc gcg ttt ggt ttg tct cag cag ctg agc att cct gct 2371
Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln Leu Ser Ile Pro Ala
745 750 755
ggc gaa gcg aag cag atc atg gag tcc tac ttc gag cgc ttc ggc gga 2419
Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe Glu Arg Phe Gly Gly
760 765 770
gta cag cgc tac ctc cgg gag atc gtg gag gag gct cga aaa gct ggc 2467
Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu Ala Arg Lys Ala Gly
775 780 785
tac acg gaa acg ctg ttt ggg cgt cgt cgc tac ctg ccg gaa ctg acc 2515
Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr Leu Pro Glu Leu Thr
790 795 800 805
tcg gat aac cgt gtc gct cgt gaa aac gct gaa cgt gcc gca ctg aac 2563
Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu Arg Ala Ala Leu Asn
810 815 820
gcc ccg att cag gga act gcc gca gac atc atc aag gtg gcc atg atc 2611
Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile Lys Val Ala Met Ile
825 830 835
cgg gtg gac cgt tca ctc aag gaa gct gcc gtg aaa tct cgc gtg ctg 2659
Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val Lys Ser Arg Val Lau
840 845 850
ctt cag gtg cat gat gaa ttg gtc gtg gaa gta gcg gcc ggt gag ttg 2707
Leu Gln Val His Asp Glu Leu Val Val Glu Val Ala Ala Gly Glu Leu
855 860 865
gaa caa gtc cgt gag att ctg gaa cgc gaa atg gat aac gcc atc aag 2755
Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met Asp Asn Ala Ile Lys
870 875 880 885
ctg tcc gtt cct ttg gaa gtt tca gct ggt gat ggc gtt aac tgg gat 2803
Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp Gly Val Asn Trp Asp
890 895 900
gct gca gcg cac taagaggtaa ctgccttttc gtcgacgagc 2845
Ala Ala Ala His
905
<2l0>4
<211>905
<212>PRT
<213>谷氨酸棒状杆菌
<400>4
Leu Gly Glu Cys Val Ala Asn Phe Ser Thr Arg Leu His Ser Gly Gly
1 5 10 15
Val Thr Glu Lys Thr Asp Gln Thr Leu Met Leu Ile Asp Gly His Ser
20 25 30
Met Ala Phe Arg Ala Phe Phe Ala Leu Pro Ala Glu Asn Phe Ser Thr
35 40 45
Ser Gly Gly Gln Ala Thr Asn Ala Val Tyr Gly Phe Leu Ser Met Leu
50 55 60
Ser Thr Leu Leu Lys Asp Glu Gln Pro Thr His Val Ala Val Ala Phe
65 70 75 80
Asp Val Gly Arg Lys Thr Phe Arg Thr Asp Met Phe Pro Ala Tyr Lys
85 90 95
Ala Gln Arg Glu Ala Thr Pro Pro Glu Phe Lys Gly Gln Val Glu Ile
100 105 110
Leu Lys Glu Val Leu Ser Thr Leu Gly Ile Thr Thr Ile Glu Lys Ile
115 120 125
Asp Phe Glu Ala Asp Asp Val Ile Ala Thr Leu Ser Val Ala Ala Lys
130 135 140
Pro Leu Gly Phe Lys Thr Leu Ile Val Thr Gly Asp Arg Asp Ser Phe
145 150 155 160
Gln Leu Val Asn Asp Thr Thr Thr Val Leu Tyr Pro Met Lys Gly Val
165 170 175
Ser Val Leu His Arg Phe Thr Pro Glu Ala Val Glu Glu Lys Tyr Gly
180 185 190
Leu Thr Pro Arg Gln Tyr Pro Glu Phe Ala Ala Leu Arg Gly Asp Pro
195 200 205
Ser Asp Asn Leu Pro Asn Ile Pro Gly Val Gly Glu Lys Thr Ala Thr
210 215 220
Lys Trp Ile Ala Gln Tyr Glu Thr Leu Asp Asn Leu Leu Asp His Ala
225 230 235 240
Asp Glu Ile Lys Gly Lys Val Gly Ala Ser Leu Arg Glu Arg Ile Glu
245 250 255
Gln Val Arg Met Asn Arg Lys Leu Thr Glu Met Val Lys Asp Leu Glu
260 265 270
Leu Pro Leu Gly Pro Asp Asp Phe Glu Met Lys Pro Val Gln Val Ala
275 280 285
Glu Val Ala Ala Lys Phe Asp Asp Leu Glu Phe Gly Thr Asn Leu Arg
290 295 300
Glu Arg Val Leu Ala Val Val Lys Ala Glu Gly Ser Ala Ala Pro Val
305 310 315 320
Glu Glu Val Glu Ala Glu Gln Val Val Val Asp Thr Gln Ser Leu Ala
325 330 335
Gln Trp Leu Pro Ala Arg Ala Gly Gln Ala Leu Ala Leu Ala Leu Ala
340 345 350
Gly Val Ala Lys Pro Ala Ala Gly Asp Thr Tyr Ala Leu Ala Ile Ala
355 360 365
Asp Thr Lys Arg His Ala Val Leu Val Asp Val Ala Asp Ile Ser Ala
370 375 380
Glu Asp Glu Lys Ala Leu Ala Thr Trp Leu Ala Ser Glu Asp Pro Lys
385 390 395 400
Met Leu His Gly Ala Lys Ala Ala Tyr His Met Leu Ala Gly Arg Gly
405 410 415
Phe Glu Leu His Gly Val Val His Asp Thr Ala Ile Ala Ala Tyr Leu
420 425 430
Leu Arg Pro Gly Gln Arg Thr Tyr Glu Leu Ala Asp Val Tyr Gln Arg
435 440 445
His Leu Gln Arg Gln Leu Ser Thr Asn Asp Asn Gly Gly Gln Leu Thr
450 455 460
Leu Leu Asp Ala Ala Asp Asp Gln Ser Leu Val Asp Asp Val Ile Ala
465 470 475 480
Ile Leu Glu Leu Ser Glu Glu Leu Thr Lys Gln Leu Gln Glu Ile Gln
485 490 495
Ala Phe Glu Leu Tyr His Asp Leu Glu Ile Pro Leu Ser Gly Ile Leu
500 505 510
Ala Arg Met Glu Ala Ile Gly Ile Ala Val Asp Val Ala Thr Leu Glu
515 520 525
Glu Gln Leu Lys Thr Phe Ile Gly Gln Val Ala Gln Glu Glu Glu Ala
530 535 540
Ala Arg Glu Leu Ala Glu Asp Pro Thr Leu Asn Leu Ser Ser Pro Lys
545 550 555 560
Gln Leu Gln Val Val Leu Phe Glu Thr Phe Gly Met Pro Lys Thr Lys
565 570 575
Lys Thr Lys Thr Gly Tyr Ser Thr Ala Ala Ala Glu Ile Glu Ala Leu
580 585 590
Ala Ile Lys Asn Pro His Pro Phe Leu Asp His Leu Leu Ala His Arg
595 600 605
Gln Tyr Gln Lys Met Lys Thr Thr Leu Glu Gly Leu Ile Arg Glu Val
610 615 620
Ala Pro Asp Gly Arg Ile His Thr Thr Phe Asn Gln Thr Val Ala Ser
625 630 635 640
Thr Gly Arg Leu Ser Ser Thr Asp Pro Asn Leu Gln Asn Ile Pro Val
645 650 655
Arg Thr Glu Ala Gly Arg Lys Ile Arg Ser Gly Phe Val Val Gly Glu
660 665 670
Gly Tyr Glu Thr Leu Leu Thr Ala Asp Tyr Ser Gln Ile Glu Met Arg
675 680 685
Val Met Ala His Leu Ser Gln Asp Pro Gly Leu Ile Glu Ala Tyr Arg
690 695 700
Glu Gly Glu Asp Leu His Asn Tyr Val Gly Ser Lys Val Phe Asn Val
705 710 715 720
Pro Ile Asp Gly Val Thr Pro Glu Leu Arg Arg Gln Val Lys Ala Met
725 730 735
Ser Tyr Gly Leu Val Tyr Gly Leu Ser Ala Phe Gly Leu Ser Gln Gln
740 745 750
Leu Ser Ile Pro Ala Gly Glu Ala Lys Gln Ile Met Glu Ser Tyr Phe
755 760 765
Glu Arg Phe Gly Gly Val Gln Arg Tyr Leu Arg Glu Ile Val Glu Glu
770 775 780
Ala Arg Lys Ala Gly Tyr Thr Glu Thr Leu Phe Gly Arg Arg Arg Tyr
785 790 795 800
Leu Pro Glu Leu Thr Ser Asp Asn Arg Val Ala Arg Glu Asn Ala Glu
805 810 815
Arg Ala Ala Leu Asn Ala Pro Ile Gln Gly Thr Ala Ala Asp Ile Ile
820 825 830
Lys Val Ala Met Ile Arg Val Asp Arg Ser Leu Lys Glu Ala Ala Val
835 840 845
Lys Ser Arg Val Leu Leu Gln Val His Asp Glu Leu Val Val Glu Val
850 855 860
Ala Ala Gly Glu Leu Glu Gln Val Arg Glu Ile Leu Glu Arg Glu Met
865 870 875 880
Asp Asn Ala Ile Lys Leu Ser Val Pro Leu Glu Val Ser Ala Gly Asp
885 890 895
Gly Val Asn Trp Asp Ala Ala Ala His
900 905
<210>5
<211>2020
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1990)
<223>RXA00157
<400>5
tttccgctac ggaacgcgga gtctttacgt ttcaattctt ggcaagtatc tagcgtcaag 60
gcttgatatg tgaagggatt cggattgaac aggagaacat gtg gcc aat cta gat 115
Val Ala Asn Leu Asp
1 5
tca gtt cgc cct ggg gtg cgg gca gct ctt cga cgt acc cat acc cag 163
Ser Val Arg Pro Gly Val Arg Ala Ala Leu Arg Arg Thr His Thr Gln
10 15 20
gta aaa ggt gtc acg ttg acc agg gcg ttg att gcg ctt gca gta agc 211
Val Lys Gly Val Thr Leu Thr Arg Ala Leu Ile Ala Leu Ala Val Ser
25 30 35
gga gct ttg ctt agt tcc atg act ccg gcg gtg gcg cag cca cag aat 259
Gly Ala Leu Leu Ser Ser Met Thr Pro Ala Val Ala Gln Pro Gln Asn
40 45 50
ccg gat gac gca gcc att gca cag gca gag gaa aat gtt tcg gcg ggc 307
Pro Asp Asp Ala Ala Ile Ala Gln Ala Glu Glu Asn Val Ser Ala Gly
55 60 65
gat ggg gaa gtc gcc cgc ctg gca gga tct ttg tcc agc act gac gcg 355
Asp Gly Glu Val Ala Arg Leu Ala Gly Ser Leu Ser Ser Thr Asp Ala
70 75 80 85
gaa att aac cgc gtc gag ctg gaa atg ggt gct ctg cgt gaa gaa gtg 403
Glu Ile Asn Arg Val Glu Leu Glu Met Gly Ala Leu Arg Glu Glu Val
90 95 100
aac aag tcc ctc gtg gat ttg cat gat gcg cag gca atc gcc gag cag 451
Asn Lys Ser Leu Val Asp Leu His Asp Ala Gln Ala Ile Ala Glu Gln
105 110 115
gcc cgc caa gat gca ctt gca gcc aag aag gat ctc gat gat tct caa 499
Ala Arg Gln Asp Ala Leu Ala Ala Lys Lys Asp Leu Asp Asp Ser Gln
120 125 130
gcg cag atc gaa gca gcc caa gag cgc ctt gat gag att tca cgt gca 547
Ala Gln Ile Glu Ala Ala Gln Glu Arg Leu Asp Glu Ile Ser Arg Ala
135 140 145
gcg tat cgc caa aac gga acc tcc aag ggg ctt tca ggc atc tcg ggc 595
Ala Tyr Arg Gln Asn Gly Thr Ser Lys Gly Leu Ser Gly Ile Ser Gly
150 155 160 165
aat gga aat tct gaa gat gcg cta gat cgt cag act tac ctg cga acc 643
Asn Gly Asn Ser Glu Asp Ala Leu Asp Arg Gln Thr Tyr Leu Arg Thr
170 175 180
agt gcg gaa aag cag cag gca gct gtt gaa gag ctt gat cgc ctc cgt 691
Ser Ala Glu Lys Gln Gln Ala Ala Val Glu Glu Leu Asp Arg Leu Arg
185 190 195
acg gaa aac gcc aac aag gaa tcg gtg ttg cgc cag gcc cgc atc gtt 739
Thr Glu Asn Ala Asn Lys Glu Ser Val Leu Arg Gln Ala Arg Ile Val
200 205 210
gct gag cag cgt gag gcg gaa gcc gtc gaa aag caa gtc cag acc gag 787
Ala Glu Gln Arg Glu Ala Glu Ala Val Glu Lys Gln Val Gln Thr Glu
215 220 225
gct gca att gcc gca aac agc gag cag ctc aat gtc ttg act aac aat 835
Ala Ala Ile Ala Ala Asn Ser Glu Gln Leu Asn Val Leu Thr Asn Asn
230 235 240 245
cgc agt acc ttg gtt gcc cag cgt gat ggg gct gag cgc aac ttg gcc 883
Arg Ser Thr Leu Val Ala Gln Arg Asp Gly Ala Glu Arg Asn Leu Ala
250 255 260
atc gct cgt gcg cag gcg gat aat ctg caa ggt cag cgt gct gag tac 931
Ile Ala Arg Ala Gln Ala Asp Asn Leu Gln Gly Gln Arg Ala Glu Tyr
26S 270 275
gag gaa ttc cag cag gca gag cag gct cgc atc cag gcg gaa gcg gaa 979
Glu Glu Phe Gln Gln Ala Glu Gln Ala Arg Ile Gln Ala Glu Ala Glu
280 285 290
gct cag gct gct gcg gag gag aag cgt cgt gcc gat gag gct gct gca 1027
Ala Gln Ala Ala Ala Glu Glu Lys Arg Arg Ala Asp Glu Ala Ala Ala
295 300 305
cag gca gcc gct gaa gct caa gaa gct gcc cag caa gct cag gcg gcg 1075
Gln Ala Ala Ala Glu Ala Gln Glu Ala Ala Gln Gln Ala Gln Ala Ala
310 315 320 325
gag gaa gcc caa gcc gcg caa gca gct gag aca gca caa gcc caa gcc 1123
Glu Glu Ala Gln Ala Ala Gln Ala Ala Glu Thr Ala Gln Ala Gln Ala
330 335 340
gcg caa gct gcg gaa acc caa gct gca caa gcc gcg caa gct cag gca 1171
Ala Gln Ala Ala Glu Thr Gln Ala Ala Gln Ala Ala Gln Ala Gln Ala
345 350 355
gaa gcg aat gat cgt gcc gcc gcg caa cag cgt gct gca gag gct caa 1219
Glu Ala Asn Asp Arg Ala Ala Ala Gln Gln Arg Ala Ala Glu Ala Gln
360 365 370
gca gca gcg gaa cag gcg caa cgt gag gct gac gct cag gcg gcc aac 1267
Ala Ala Ala Glu Gln Ala Gln Arg Glu Ala Asp Ala Gln Ala Ala Asn
375 380 385
gat gcc caa gct cag gca ctg cgt gaa cag gcg ctc acc gca gcc tcc 1315
Asp Ala Gln Ala Gln Ala Leu Arg Glu Gln Ala Leu Thr Ala Ala Ser
390 395 400 405
atc gct gcg gct gct cta att gcg gcg agc cag tcc agc cat gcc act 1363
Ile Ala Ala Ala Ala Leu Ile Ala Ala Ser Gln Ser Ser His Ala Thr
410 415 420
act caa aat cct tac cca act gat gaa gac gcg gat ccg acc gat att 1411
Thr Gln Asn Pro Tyr Pro Thr Asp Glu Asp Ala Asp Pro Thr Asp Ile
425 430 435
gcg gac atc caa ggc cca acg cag cca ggt acg ggt gag tct gga gat 1459
Ala Asp Ile Gln Gly Pro Thr Gln Pro Gly Thr Gly Glu Ser Gly Asp
440 445 450
tcc cag agc aac tcc agc gac aac gat tcc aca ggc aac gat tcc aca 1507
Ser Gln Ser Asn Ser Ser Asp Asn Asp Ser Thr Gly Asn Asp Ser Thr
455 460 465
ggc tct gac tct tca gat tca gat tcc tcc ggc aac gat tct tca gag 1555
Gly Ser Asp Ser Ser Asp Ser Asp Ser Ser Gly Asn Asp Ser Ser Glu
470 475 480 485
gtt att tcc ggc gat cgt tcc gct cag att gag act gtg att gcg cgc 1603
Val Ile Ser Gly Asp Arg Ser Ala Gln Ile Glu Thr Val Ile Ala Arg
490 495 500
gcc atg agc cag ttg ggt gtg cag tac gca tgg ggt ggc ggt aac gct 1651
Ala Met Ser Gln Leu Gly Val Gln Tyr Ala Trp Gly Gly Gly Asn Ala
505 510 515
aat ggc cca act ctg ggt atc cgt gac ggt ggc gtg gcg gac tct tac 1699
Asn Gly Pro Thr Leu Gly Ile Arg Asp Gly Gly Val Ala Asp Ser Tyr
520 525 530
ggc gat tac aac aag gtt ggc ttc gac tgc tct gga ctg acc ttg tat 1747
Gly Asp Tyr Asn Lys Val Gly Phe Asp Cys Ser Gly Leu Thr Leu Tyr
535 540 545
gcg ttt gcg ggt gtg gga att tca ctt cct cac tac acg ggc tac cag 1795
Ala Phe Ala Gly Val Gly Ile Ser Leu Pro His Tyr Thr Gly Tyr Gln
550 555 560 565
tac cag cac ggc acc aag gtg tcg cct tct gag atg caa cgt ggc gat 1843
Tyr Gln His Gly Thr Lys Val Ser Pro Ser Glu Met Gln Arg Gly Asp
570 575 580
ctg atc ttc tat ggt ccg gga gcg tct cag cac gtg gca att tac ctc 1891
Leu Ile Phe Tyr Gly Pro Gly Ala Ser Gln His Val Ala Ile Tyr Leu
585 590 595
ggt gat ggt cag atg att gag gct ccg aat tcg ggt tct gtc gtg aag 1939
Gly Asp Gly Gln Met Ile Glu Ala Pro Asn Ser Gly Ser Val Val Lys
600 605 610
att tct cct gtt cgc tgg agc gga atg acc gag agc gtg gta cgc ctc 1987
Ile Ser Pro Val Arg Trp Ser Gly Met Thr Glu Ser Val Val Arg Leu
615 620 625
att tagtttcctc ctatgaatct tgatgtggtt 2020
Ile
630
<210>6
<211>630
<212>PRT
<213>谷氨酸棒状杆菌
<400>6
Val Ala Asn Leu Asp Ser Val Arg Pro Gly Val Arg Ala Ala Leu Arg
1 5 10 15
Arg Thr His Thr Gln Val Lys Gly Val Thr Leu Thr Arg Ala Leu Ile
20 25 30
Ala Leu Ala Val Ser Gly Ala Leu Leu Ser Ser Met Thr Pro Ala Val
35 40 45
Ala Gln Pro Gln Asn Pro Asp Asp Ala Ala Ile Ala Gln Ala Glu Glu
50 55 60
Asn Val Ser Ala Gly Asp Gly Glu Val Ala Arg Leu Ala Gly Ser Leu
65 70 75 80
Ser Ser Thr Asp Ala Glu Ile Asn Arg Val Glu Leu Glu Met Gly Ala
85 90 95
Leu Arg Glu Glu Val Asn Lys Ser Leu Val Asp Leu His Asp Ala Gln
100 105 110
Ala Ile Ala Glu Gln Ala Arg Gln Asp Ala Leu Ala Ala Lys Lys Asp
115 120 125
Leu Asp Asp Ser Gln Ala Gln Ile Glu Ala Ala Gln Glu Arg Leu Asp
130 135 140
Glu Ile Ser Arg Ala Ala Tyr Arg Gln Asn Gly Thr Ser Lys Gly Leu
145 150 155 160
Ser Gly Ile Ser Gly Asn Gly Asn Ser Glu Asp Ala Leu Asp Arg Gln
165 170 175
Thr Tyr Leu Arg Thr Ser Ala Glu Lys Gln Gln Ala Ala Val Glu Glu
180 185 190
Leu Asp Arg Leu Arg Thr Glu Asn Ala Asn Lys Glu Ser Val Leu Arg
195 200 205
Gln Ala Arg Ile Val Ala Glu Gln Arg Glu Ala Glu Ala Val Glu Lys
210 215 220
Gln Val Gln Thr Glu Ala Ala Ile Ala Ala Asn Ser Glu Gln Leu Asn
225 230 235 240
Val Leu Thr Asn Asn Arg Ser Thr Leu Val Ala Gln Arg Asp Gly Ala
245 250 255
Glu Arg Asn Leu Ala Ile Ala Arg Ala Gln Ala Asp Asn Leu Gln Gly
260 265 270
Gln Arg Ala Glu Tyr Glu Glu Phe Gln Gln Ala Glu Gln Ala Arg Ile
275 280 285
Gln Ala Glu Ala Glu Ala Gln Ala Ala Ala Glu Glu Lys Arg Arg Ala
290 295 300
Asp Glu Ala Ala Ala Gln Ala Ala Ala Glu Ala Gln Glu Ala Ala Gln
305 310 315 320
Gln Ala Gln Ala Ala Glu Glu Ala Gln Ala Ala Gln Ala Ala Glu Thr
325 330 335
Ala Gln Ala Gln Ala Ala Gln Ala Ala Glu Thr Gln Ala Ala Gln Ala
340 345 350
Ala Gln Ala Gln Ala Glu Ala Asn Asp Arg Ala Ala Ala Gln Gln Arg
355 360 365
Ala Ala Glu Ala Gln Ala Ala Ala Glu Gln Ala Gln Arg Glu Ala Asp
370 375 380
Ala Gln Ala Ala Asn Asp Ala Gln Ala Gln Ala Leu Arg Glu Gln Ala
385 390 395 400
Leu Thr Ala Ala Ser Ile Ala Ala Ala Ala Leu Ile Ala Ala Ser Gln
405 410 415
Ser Ser His Ala Thr Thr Gln Asn Pro Tyr Pro Thr Asp Glu Asp Ala
420 425 430
Asp Pro Thr Asp Ile Ala Asp Ile Gln Gly Pro Thr Gln Pro Gly Thr
435 440 445
Gly Glu Ser Gly Asp Ser Gln Ser Asn Ser Ser Asp Asn Asp Ser Thr
450 455 460
Gly Asn Asp Ser Thr Gly Ser Asp Ser Ser Asp Ser Asp Ser Ser Gly
465 470 475 480
Asn Asp Ser Ser Glu Val Ile Ser Gly Asp Arg Ser Ala Gln Ile Glu
485 490 495
Thr Val Ile Ala Arg Ala Met Ser Gln Leu Gly Val Gln Tyr Ala Trp
500 505 510
Gly Gly Gly Asn Ala Asn Gly Pro Thr Leu Gly Ile Arg Asp Gly Gly
515 520 525
Val Ala Asp Ser Tyr Gly Asp Tyr Asn Lys Val Gly Phe Asp Cys Ser
530 535 540
Gly Leu Thr Leu Tyr Ala Phe Ala Gly Val Gly Ile Ser Leu Pro His
545 550 555 560
Tyr Thr Gly Tyr Gln Tyr Gln His Gly Thr Lys Val Ser Pro Ser Glu
565 570 575
Met Gln Arg Gly Asp Leu Ile Phe Tyr Gly Pro Gly Ala Ser Gln His
580 585 590
Val Ala Ile Tyr Leu Gly Asp Gly Gln Met Ile Glu Ala Pro Asn Ser
595 600 605
Gly Ser Val Val Lys Ile Ser Pro Val Arg Trp Ser Gly Met Thr Glu
610 615 620
Ser Val Val Arg Leu Ile
625 630
<210>7
<211>979
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(949)
<223>RXA00208
<400>7
cggccaattt tttcttttgt gggggtgcct cttacgcatt tcctgaattt ttgttaggct 60
tgcctaggtc agttaaagat atatcgataa gaggttttcc atg aac act cca gcg 115
Met Asn Thr Pro Ala
1 5
ccc cgc aaa cga cgc gaa atg aaa atc aag aca gca acc gtc act ggt 163
Pro Arg Lys Arg Arg Glu Met Lys Ile Lys Thr Ala Thr Val Thr Gly
10 15 20
gtg cgt caa att tcc ccc gat ctc atc cgc ttc agc ttc gac tgc cca 211
Val Arg Gln Ile Ser Pro Asp Leu Ile Arg Phe Ser Phe Asp Cys Pro
25 30 35
gaa atc gtt ggc gcc gac ctg ggg ttc acg gac cat tac atc aag atc 259
Glu Ile Val Gly Ala Asp Leu Gly Phe Thr Asp His Tyr Ile Lys Ile
40 45 50
ctc ttc gtg cca gca ggt gcg gat tac tcc tgg cct ttc gac atg gca 307
Leu Phe Val Pro Ala Gly Ala Asp Tyr Ser Trp Pro Phe Asp Met Ala
55 60 65
gaa att gcg gaa acc cag ccc cgt gag ctg caa cca gtg cgc cgc acc 355
Glu Ile Ala Glu Thr Gln Pro Arg Glu Leu Gln Pro Val Arg Arg Thr
70 75 80 85
tac act ttc cgc acg gtt gac act gtc gca ggc aca ttt gac ata gat 403
Tyr Thr Phe Arg Thr Val Asp Thr Val Ala Gly Thr Phe Asp Ile Asp
90 95 100
ttc gtt gcg cac ggc acc gat ggc ctt gcc ggt cct tgg gcg cag cag 451
Phe Val Ala His Gly Thr Asp Gly Leu Ala Gly Pro Trp Ala Gln Gln
105 110 115
gca cag gta ggt gat gtc atc gcg ttc ggc ggc cca ggt ggc gca tgg 499
Ala Gln Val Gly Asp Val Ile Ala Phe Gly Gly Pro Gly Gly Ala Trp
120 125 130
aag cca gaa acc acc tat gag cac tac gtt ctc gca ggc gat gaa gcc 547
Lys Pro Glu Thr Thr Tyr Glu His Tyr Val Leu Ala Gly Asp Glu Ala
135 140 145
gca gca ccc gca att ttc gca gcc tta gaa cac cta ccc gcc ggc acc 595
Ala Ala Pro Ala Ile Phe Ala Ala Leu Glu His Leu Pro Ala Gly Thr
150 155 160 165
acc gcc aaa gcc ttc att gaa atc tcc tcc aac gaa gcg cgt ttc aac 643
Thr Ala Lys Ala Phe Ile Glu Ile Ser Ser Asn Glu Ala Arg Phe Asn
170 175 180
gcc cca gcc agc gac aac atc gag gtt gtc tgg gtg ccc cgc gac ggc 691
Ala Pro Ala Ser Asp Asn Ile Glu Val Val Trp Val Pro Arg Asp Gly
185 190 195
gcc acc cac ggt aca ttg ctt atc gac gcc ctc cgc cag gac ggc tac 739
Ala Thr His Gly Thr Leu Leu Ile Asp Ala Leu Arg Gln Asp Gly Tyr
200 205 210
cca acc aag aaa act tcc tgg ttc atc cac gga gtc gcc gaa atg gtg 787
Pro Thr Lys Lys Thr Ser Trp Phe Ile His Gly Val Ala Glu Met Val
215 220 225
aag gaa acc cgc aaa ttc ctc ttc gtg gaa ggc aac gta gac aaa gct 835
Lys Glu Thr Arg Lys Phe Leu Phe Val Glu Gly Asn Val Asp Lys Ala
230 235 240 245
gat gca tcc att tcc gga tac tgg cgc ctt ggc atg acc gaa gac cag 833
Asp Ala Ser Ile Ser Gly Tyr Trp Arg Leu Gly Met Thr Glu Asp Gln
250 255 260
tgg cag gcc tcc aag cgg gag ttc aat gag caa aat gag gca gaa gaa 931
Trp Gln Ala Ser Lys Arg Glu Phe Asn Glu Gln Asn Glu Ala Glu Glu
265 270 275
ctc gcg ctc agc aag gca taagggcaag gggttctaga aagaccaacg 979
Leu Ala Leu Ser Lys Ala
280
<210>8
<211>283
<212>PRT
<213>谷氨酸棒状杆菌
<400>8
Met Asn Thr Pro Ala Pro Arg Lys Arg Arg Glu Met Lys Ile Lys Thr
1 5 10 15
Ala Thr Val Thr Gly Val Arg Gln Ile Ser Pro Asp Lau Ile Arg Phe
20 25 30
Ser Phe Asp Cys Pro Glu Ile Val Gly Ala Asp Leu Gly Phe Thr Asp
35 40 45
His Tyr Ile Lys Ile Leu Phe Val Pro Ala Gly Ala Asp Tyr Ser Trp
50 55 60
Pro Phe Asp Met Ala Glu Ile Ala Glu Thr Gln Pro Arg Glu Leu Gln
65 70 75 80
Pro Val Arg Arg Thr Tyr Thr Phe Arg Thr Val Asp Thr Val Ala Gly
85 90 95
Thr Phe Asp Ile Asp Phe Val Ala His Gly Thr Asp Gly Leu Ala Gly
100 105 110
Pro Trp Ala Gln Gln Ala Gln Val Gly Asp Val Ile Ala Phe Gly Gly
115 120 125
Pro Gly Gly Ala Trp Lys Pro Glu Thr Thr Tyr Glu His Tyr Val Leu
130 135 140
Ala Gly Asp Glu Ala Ala Ala Pro Ala Ile Phe Ala Ala Leu Glu His
145 150 155 160
Leu Pro Ala Gly Thr Thr Ala Lys Ala Phe Ile Glu Ile Ser Ser Asn
165 170 175
Glu Ala Arg Phe Asn Ala Pro Ala Ser Asp Asn Ile Glu Val Val Trp
180 185 190
Val Pro Arg Asp Gly Ala Thr His Gly Thr Leu Leu Ile Asp Ala Leu
195 200 205
Arg Gln Asp Gly Tyr Pro Thr Lys Lys Thr Ser Trp Phe Ile His Gly
210 215 220
Val Ala Glu Met Val Lys Glu Thr Arg Lys Phe Leu Phe Val Glu Gly
225 230 235 240
Asn Val Asp Lys Ala Asp Ala Ser Ile Ser Gly Tyr Trp Arg Leu Gly
245 250 255
Met Thr Glu Asp Gln Trp Gln Ala Ser Lys Arg Glu Phe Asn Glu Gln
260 265 270
Asn Glu Ala Glu Glu Leu Ala Leu Ser Lys Ala
275 280
<210>9
<211>1069
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1039)
<223>RXA00313
<400>9
gatcgtcttg ccgatgctgg tattgaggtt gtggataccg cagatggcgc tacatggaaa 60
ttgcagtaat tacagacact tttaaggaga taatttaaac atg gca gga aat gac 115
Met Ala Gly Asn Asp
1 5
agt cgt cga ggc ggc tta cgc aag acc aat aaa aaa ggt gca acc aag 163
Ser Arg Arg Gly Gly Leu Arg Lys Thr Asn Lys Lys Gly Ala Thr Lys
10 15 20
ggc agt ggc gga cag gtt cgt cgc ggt ctg aaa ggt aag ggg cct acc 211
Gly Ser Gly Gly Gln Val Arg Arg Gly Leu Lys Gly Lys Gly Pro Thr
25 30 35
cct aaa gct gag gat cgc acc tat cac gca gct cac aag cgc aag gtg 259
Pro Lys Ala Glu Asp Arg Thr Tyr His Ala Ala His Lys Arg Lys Val
40 45 50
gag cgt gat cgt cgt gat cgt gga cgc cac cag cgt gaa atg cca gag 307
Glu Arg Asp Arg Arg Asp Arg Gly Arg His Gln Arg Glu Met Pro Glu
55 60 65
ttg gtt gtg ggc cgt aac cca gtg ctg gaa tgt ctg cat gca cgc gtt 355
Leu Val Val Gly Arg Asn Pro Val Leu Glu Cys Leu His Ala Arg Val
70 75 80 85
cca gcg act gct ttg tat gtt gca gag ggt gcg gcg aac gat gag cgt 403
Pro Ala Thr Ala Leu Tyr Val Ala Glu Gly Ala Ala Asn Asp Glu Arg
90 95 100
ctg agc gag gca gtg cac act gcg gct ggc cga aat ctt cca gtg ctg 451
Leu Ser Glu Ala Val His Thr Ala Ala Gly Arg Asn Leu Pro Val Leu
105 110 115
gag gtt aac aag ctg gag ctg gat cgt atg acc ggc aac ggc atg cac 499
Glu Val Asn Lys Leu Glu Leu Asp Arg Met Thr Gly Asn Gly Met His
120 125 130
cag ggc atc ggc ctg gcg atc cct cct tac gag tac gca gat gtt cat 547
Gln Gly Ile Gly Leu Ala Ile Pro Pro Tyr Glu Tyr Ala Asp Val His
135 140 145
gat ctg atc gcc aat gct gcg gct tct aag aag cca ggc atg ttc gtg 595
Asp Leu Ile Ala Asn Ala Ala Ala Ser Lys Lys Pro Gly Met Phe Val
150 155 160 165
att ctg gat aac atc acc gac cca cgt aac ttg ggt gct gtg att cgt 643
Ile Leu Asp Asn Ile Thr Asp Pro Arg Asn Leu Gly Ala Val Ile Arg
170 175 180
tcc gtc ggt gct ttc ggc ggc aac ggt gtc atc att ccg gag cgt cgt 691
Ser Val Gly Ala Phe Gly Gly Asn Gly Val Ile Ile Pro Glu Arg Arg
185 190 195
tca gca tct gtg acc gct gtt gca tgg cgt act tct gct ggt acc gca 739
Ser Ala Ser Val Thr Ala Val Ala Trp Arg Thr Ser Ala Gly Thr Ala
200 205 210
gcg cgt gtg cca gtg gcg aag gaa acc aac atg act cgt gtc gtg aag 787
Ala Arg Val Pro Val Ala Lys Glu Thr Asn Met Thr Arg Val Val Lys
215 220 225
gaa ttc cag caa aac ggt tac cag gtc gtc ggc ctt gac gct ggc ggc 835
Glu Phe Gln Gln Asn Gly Tyr Gln Val Val Gly Leu Asp Ala Gly Gly
230 235 240 245
gac cac act ttg gac acc tac gac ggc acc gac aac gtt gtc atc gtc 883
Asp His Thr Leu Asp Thr Tyr Asp Gly Thr Asp Asn Val Val Ile Val
250 255 260
gtc ggt tct gag ggc aag gga att tcc cgt ctc gtt cgc gaa aac tgc 931
Val Gly Ser Glu Gly Lys Gly Ile Ser Arg Leu Val Arg Glu Asn Cys
265 270 275
gac acc atc atg tcc ata ccc acc gag ggc tgg gtt gaa tcg ctg aac 979
Asp Thr Ile Met Ser Ile Pro Thr Glu Gly Trp Val Glu Ser Leu Asn
280 285 290
gct tcg gtt gct gcc ggc gtc gtg ctg tcg gag ttc tcg cgc cag cgt 1027
Ala Ser Val Ala Ala Gly Val Val Leu Ser Glu Phe Ser Arg Gln Arg
295 300 305
cgc att aag ggt taagccggag gttggcgtcg aaaagccaaa 1069
Arg Ile Lys Gly
310
<210>10
<211>313
<212>PRT
<213>谷氨酸棒状杆菌
<400>10
Met Ala Gly Asn Asp Ser Arg Arg Gly Gly Leu Arg Lys Thr Asn Lys
1 5 10 15
Lys Gly Ala Thr Lys Gly Ser Gly Gly Gln Val Arg Arg Gly Leu Lys
20 25 30
Gly Lys Gly Pro Thr Pro Lys Ala Glu Asp Arg Thr Tyr His Ala Ala
35 40 45
His Lys Arg Lys Val Glu Arg Asp Arg Arg Asp Arg Gly Arg His Gln
50 55 60
Arg Glu Met Pro Glu Leu Val Val Gly Arg Asn Pro Val Leu Glu Cys
65 70 75 80
Leu His Ala Arg Val Pro Ala Thr Ala Leu Tyr Val Ala Glu Gly Ala
85 90 95
Ala Asn Asp Glu Arg Leu Ser Glu Ala Val His Thr Ala Ala Gly Arg
100 105 110
Asn Leu Pro Val Leu Glu Val Asn Lys Leu Glu Leu Asp Arg Met Thr
115 120 125
Gly Asn Gly Met His Gln Gly Ile Gly Leu Ala Ile Pro Pro Tyr Glu
130 135 140
Tyr Ala Asp Val His Asp Leu Ile Ala Asn Ala Ala Ala Ser Lys Lys
145 150 155 160
Pro Gly Met Phe Val Ile Leu Asp Asn Ile Thr Asp Pro Arg Asn Leu
165 170 175
Gly Ala Val Ile Arg Ser Val Gly Ala Phe Gly Gly Asn Gly Val Ile
180 185 190
Ile Pro Glu Arg Arg Ser Ala Ser Val Thr Ala Val Ala Trp Arg Thr
195 200 205
Ser Ala Gly Thr Ala Ala Arg Val Pro Val Ala Lys Glu Thr Asn Met
210 215 220
Thr Arg Val Val Lys Glu Phe Gln Gln Asn Gly Tyr Gln Val Val Gly
225 230 235 240
Leu Asp Ala Gly Gly Asp His Thr Leu Asp Thr Tyr Asp Gly Thr Asp
245 250 255
Asn Val Val Ile Val Val Gly Ser Glu Gly Lys Gly Ile Ser Arg Leu
260 265 270
Val Arg Glu Asn Cys Asp Thr Ile Met Ser Ile Pro Thr Glu Gly Trp
275 280 285
Val Glu Ser Leu Asn Ala Ser Val Ala Ala Gly Val Val Leu Ser Glu
290 295 300
Phe Ser Arg Gln Arg Arg Ile Lys Gly
305 310
<210>11
<211>1645
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1615)
<223>RXA00341
<400>11
cgggaatgtg gtgagtcctt gatcagtaaa cctgcagaaa tcacatttgc cccaccagtc 60
ccaaaatgga accctctcag aatcgctttt aacgggctaa atg agc tcg acc cat 115
Met Ser Ser Thr His
1 5
acc aag acc tat ctg ggg aac ctc gac cct tct gtg ccc aag gaa act 163
Thr Lys Thr Tyr Leu Gly Asn Leu Asp Pro Ser Val Pro Lys Glu Thr
10 15 20
caa cac ccc tgc ata tac ttt tcc gct gtg gaa caa tgg gag cgc atg 211
Gln His Pro Cys Ile Tyr Phe Ser Ala Val Glu Gln Trp Glu Arg Met
25 30 35
aag ctc tat gca gca gtc ctc gac ttt gaa cca gtg gca caa gag ttc 259
Lys Leu Tyr Ala Ala Val Leu Asp Phe Glu Pro Val Ala Gln Glu Phe
40 45 50
ggt gtg gag cga ggt ttt gac cct cat atc cac gac gaa gcc gcg tca 307
Gly Val Glu Arg Gly Phe Asp Pro His Ile His Asp Glu Ala Ala Ser
55 60 65
agt gtc gat agg tat gcg caa gag cgg gaa gat ctc ctg cac atg ccc 355
Ser Val Asp Arg Tyr Ala Gln Glu Arg Glu Asp Leu Leu His Met Pro
70 75 80 85
ttt gtc acc atc gat ccc gta ggt tcc aga gac ctc gat caa gct gtg 403
Phe Val Thr Ile Asp Pro Val Gly Ser Arg Asp Leu Asp Gln Ala Val
90 95 100
ctg att gag gag atc gac agc gga ttt cgg gtg cat tac gcg att gca 451
Leu Ile Glu Glu Ile Asp Ser Gly Phe Arg Val His Tyr Ala Ile Ala
105 110 115
gat gtc gca gcc ttc gtg gag ccg ggc agt gaa ttg gaa aag att tcc 499
Asp Val Ala Ala Phe Val Glu Pro Gly Ser Glu Leu Glu Lys Ile Ser
120 125 130
ctt cac cgc ggg cag act att tat ctg ccg gat tcc cca gcg cga ctg 547
Leu His Arg Gly Gln Thr Ile Tyr Leu Pro Asp Ser Pro Ala Arg Leu
135 140 145
cac cct gag gaa tta tcc gaa gat gcg gca agc ctg ctg gag gga caa 595
His Pro Glu Glu Leu Ser Glu Asp Ala Ala Ser Leu Leu Glu Gly Gln
150 155 160 165
acg aga cca gcg gtt gtg tgg tcg att gat cta gat gaa cgt ggc gaa 643
Thr Arg Pro Ala Val Val Trp Ser Ile Asp Leu Asp Glu Arg Gly Glu
170 175 180
gtc aca gcc acc aag gtg cgt cgc ggg ttg gtg aaa tcc cgg gcg cgt 691
Val Thr Ala Thr Lys Val Arg Arg Gly Leu Val Lys Ser Arg Ala Arg
185 190 195
ttg gat tat gat cag gct caa ata gat gcc gag aat ggt cgg ttg cat 739
Leu Asp Tyr Asp Gln Ala Gln Ile Asp Ala Glu Asn Gly Arg Leu His
200 205 210
ccg tcg ata agc tta ttg ccc aag gtc ggg cag ctg agg cag gaa agc 787
Pro Ser Ile Ser Leu Leu Pro Lys Val Gly Gln Leu Arg Gln Glu Ser
215 220 225
gcg cta cgg cgc gaa gcc gtg aat ctt tct att ccc agc cag cga gtg 835
Ala Leu Arg Arg Glu Ala Val Asn Leu Ser Ile Pro Ser Gln Arg Val
230 235 240 245
gtg aaa gtg ccc aat gat gac gcc ggt gaa cac tat gaa att gtc atc 883
Val Lys Val Pro Asn Asp Asp Ala Gly Glu His Tyr Glu Ile Val Ile
250 255 260
gag cca cgc ccg cac atc atg gat tac aat tcc gag att tcc ctg ctc 931
Glu Pro Arg Pro His Ile Met Asp Tyr Asn Ser Glu Ile Ser Leu Leu
265 270 275
aca ggc atg gta gcg ggg gag atg atg gtg aaa gcg ggg cac ggt ttg 979
Thr Gly Met Val Ala Gly Glu Met Met Val Lys Ala Gly His Gly Leu
280 285 290
ctg cgt aca ctc gcc ccg gcg acc aaa gaa tcc gaa gct act ttc aga 1027
Leu Arg Thr Leu Ala Pro Ala Thr Lys Glu Ser Glu Ala Thr Phe Arg
295 300 305
tca gag gcg caa gcc ctt ggt ttt gag atc gcg ccc gaa caa ccc atc 1075
Ser Glu Ala Gln Ala Leu Gly Phe Glu Ile Ala Pro Glu Gln Pro Ile
310 315 320 325
ggt gag ttt ctt caa agt gtg gat ccc aat acg ccc aaa ggg atg gcc 1123
Gly Glu Phe Leu Gln Ser Val Asp Pro Asn Thr Pro Lys Gly Met Ala
330 335 340
att cag agg gaa gca cag aaa ctc ttg cgg ggc tcc ggc tac gcc agc 1171
Ile Gln Arg Glu Ala Gln Lys Leu Leu Arg Gly Ser Gly Tyr Ala Ser
345 350 355
gtg aaa aat ggg gac tcg gaa gtg cat tcc ggt gtt ggt ggt tac tat 1219
Val Lys Asn Gly Asp Ser Glu Val His Ser Gly Val Gly Gly Tyr Tyr
360 365 370
gct cac gtc acc gca ccg ctg cgc cga ctt atc gac cgt ttc gcc acc 1267
Ala His Val Thr Ala Pro Leu Arg Arg Leu Ile Asp Arg Phe Ala Thr
375 380 385
gaa cat tgc ctt gcg att gcc tcc gga acg gac gtt cct gaa tgg gtg 1315
Glu His Cys Leu Ala Ile Ala Ser Gly Thr Asp Val Pro Glu Trp Val
390 395 400 405
acc agg gtg gaa gag caa gtt ctc gac acc atg aaa tac tcc tcc att 1363
Thr Arg Val Glu Glu Gln Val Leu Asp Thr Met Lys Tyr Ser Ser Ile
410 415 420
ttg gcc agc caa gtg gat aat gcc tgc ctc gac ctc aca gaa gcc acc 1411
Leu Ala Ser Gln Val Asp Asn Ala Cys Leu Asp Leu Thr Glu Ala Thr
425 430 435
gtg ttg aaa tac tgg gag ggc caa aac ttc aac gcg gtg gtt gta gcg 1459
Val Leu Lys Tyr Trp Glu Gly Gln Asn Phe Asn Ala Val Val Val Ala
440 445 450
agc gaa cct gaa aag aac tct gct cga ctt ttt gtg tac aaa ccg cca 1507
Ser Glu Pro Glu Lys Asn Ser Ala Arg Leu Phe Val Tyr Lys Pro Pro
455 460 465
gtg ttg gca aag tgt att ggc gcc cca gaa cag gga aca aac caa gat 1555
Val Leu Ala Lys Cys Ile Gly Ala Pro Glu Gln Gly Thr Asn Gln Asp
470 475 480 485
gtc aca ctg gtg act gcg aac ttg aag aag cgt gaa gtt ttg ttt gcg 1603
Val Thr Leu Val Thr Ala Asn Leu Lys Lys Arg Glu Val Leu Phe Ala
490 495 500
tgg ccg gct gac taagcatgca ggctggttaa gtaggcgtgc 1645
Trp Pro Ala Asp
505
<210>12
<211>505
<212>PRT
<213>谷氨酸棒状杆菌
<400>12
Met Ser Ser Thr His Thr Lys Thr Tyr Leu Gly Asn Leu Asp Pro Ser
1 5 10 15
Val Pro Lys Glu Thr Gln His Pro Cys Ile Tyr Phe Ser Ala Val Glu
20 25 30
Gln Trp Glu Arg Met Lys Leu Tyr Ala Ala Val Leu Asp Phe Glu Pro
35 40 45
Val Ala Gln Glu Phe Gly Val Glu Arg Gly Phe Asp Pro His Ile His
50 55 60
Asp Glu Ala Ala Ser Ser Val Asp Arg Tyr Ala Gln Glu Arg Glu Asp
65 70 75 80
Leu Leu His Met Pro Phe Val Thr Ile Asp Pro Val Gly Ser Arg Asp
85 90 95
Leu Asp Gln Ala Val Leu Ile Glu Glu Ile Asp Ser Gly Phe Arg Val
100 105 110
His Tyr Ala Ile Ala Asp Val Ala Ala Phe Val Glu Pro Gly Ser Glu
115 120 125
Leu Glu Lys Ile Ser Leu His Arg Gly Gln Thr Ile Tyr Leu Pro Asp
130 135 140
Ser Pro Ala Arg Leu His Pro Glu Glu Leu Ser Glu Asp Ala Ala Ser
145 150 155 160
Leu Leu Glu Gly Gln Thr Arg Pro Ala Val Val Trp Ser Ile Asp Leu
165 170 175
Asp Glu Arg Gly Glu Val Thr Ala Thr Lys Val Arg Arg Gly Leu Val
180 185 190
Lys Ser Arg Ala Arg Leu Asp Tyr Asp Gln Ala Gln Ile Asp Ala Glu
195 200 205
Asn Gly Arg Leu His Pro Ser Ile Ser Leu Leu Pro Lys Val Gly Gln
210 215 220
Leu Arg Gln Glu Ser Ala Leu Arg Arg Glu Ala Val Asn Leu Ser Ile
225 230 235 240
Pro Ser Gln Arg Val Val Lys Val Pro Asn Asp Asp Ala Gly Glu His
245 250 255
Tyr Glu Ile Val Ile Glu Pro Arg Pro His Ile Met Asp Tyr Asn Ser
260 265 270
Glu Ile Ser Leu Leu Thr Gly Met Val Ala Gly Glu Met Met Val Lys
275 280 285
Ala Gly His Gly Leu Leu Arg Thr Leu Ala Pro Ala Thr Lys Glu Ser
290 295 300
Glu Ala Thr Phe Arg Ser Glu Ala Gln Ala Leu Gly Phe Glu Ile Ala
305 310 315 320
Pro Glu Gln Pro Ile Gly Glu Phe Leu Gln Ser Val Asp Pro Asn Thr
325 330 335
Pro Lys Gly Met Ala Ile Gln Arg Glu Ala Gln Lys Leu Leu Arg Gly
340 345 350
Ser Gly Tyr Ala Ser Val Lys Asn Gly Asp Ser Glu Val His Ser Gly
355 360 365
Val Gly Gly Tyr Tyr Ala His Val Thr Ala Pro Leu Arg Arg Leu Ile
370 375 380
Asp Arg Phe Ala Thr Glu His Cys Leu Ala Ile Ala Ser Gly Thr Asp
385 390 395 400
Val Pro Glu Trp Val Thr Arg Val Glu Glu Gln Val Leu Asp Thr Met
405 410 415
Lys Tyr Ser Ser Ile Leu Ala Ser Gln Val Asp Asn Ala Cys Leu Asp
420 425 430
Leu Thr Glu Ala Thr Val Leu Lys Tyr Trp Glu Gly Gln Asn Phe Asn
435 440 445
Ala Val Val Val Ala Ser Glu Pro Glu Lys Asn Ser Ala Arg Leu Phe
450 455 460
Val Tyr Lys Pro Pro Val Leu Ala Lys Cys Ile Gly Ala Pro Glu Gln
465 470 475 480
Gly Thr Asn Gln Asp Val Thr Leu Val Thr Ala Asn Leu Lys Lys Arg
485 490 495
Glu Val Leu Phe Ala Trp Pro Ala Asp
500 505
<210>13
<211>3295
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3265)
<223>RXA00407
<400>13
agtatttagg agattagcac catagaccgc gatctgtaca gcttggtcta tttccttctc 60
cggctggcgt ggaaaactta ttggaaaatt tgttctaaaa atg tat cct atc att 115
Met Tyr Pro Ile Ile
1 5
atg gaa tgg aat ggc ggg gga agc ttt aac ggc agg cca tta tcg tgg 163
Met Glu Trp Asn Gly Gly Gly Ser Phe Asn Gly Arg Pro Leu Ser Trp
10 15 20
tcc aaa ttg gaa aga atc ctc tca ggt aaa aag gtc gaa tct ctg cgt 211
Ser Lys Leu Glu Arg Ile Leu Ser Gly Lys Lys Val Glu Ser Leu Arg
25 30 35
ccg gtg ctg cat gag ccg gat gcg cag gcg cct tca tct gcc atg cag 259
Pro Val Leu His Glu Pro Asp Ala Gln Ala Pro Ser Ser Ala Met Gln
40 45 50
ggg gaa gcg tcg gtt cct ttt gcc gag ctg cat gcc acc agc agt tat 307
Gly Glu Ala Ser Val Pro Phe Ala Glu Leu His Ala Thr Ser Ser Tyr
55 60 65
aac ttt ctc act ggc gca tcg gat ccg tct gat gtg gtt gtg cag gcc 355
Asn Phe Leu Thr Gly Ala Ser Asp Pro Ser Asp Val Val Val Gln Ala
70 75 80 85
aaa aag tta gga ctt gtt gct cta tca gtc atg gat agg gat ggt ttt 403
Lys Lys Leu Gly Leu Val Ala Leu Ser Val Met Asp Arg Asp Gly Phe
90 95 100
tat ggt gca gtg aga ttt gcg gaa gct gcc gcg gaa gct gga atg cat 451
Tyr Gly Ala Val Arg Phe Ala Glu Ala Ala Ala Glu Ala Gly Met His
105 110 115
acc gtt tat ggt gcg gag ctg agt ctg caa gaa ggc gtg ttg aca gtc 499
Thr Val Tyr Gly Ala Glu Leu Ser Leu Gln Glu Gly Val Leu Thr Val
120 125 130
ttg tgt aaa aat ccg gaa ggc tac aaa aag ctc agt cac ctg atc agt 547
Leu Cys Lys Asn Pro Glu Gly Tyr Lys Lys Leu Ser His Leu Ile Ser
135 140 145
gac gcg aaa atg gca acg gga gaa aag ggg gaa gtt cgc tat ccg ccg 595
Asp Ala Lys Met Ala Thr Gly Glu Lys Gly Glu Val Arg Tyr Pro Pro
150 155 160 165
ctg cca atg gtt gct gaa cat gct gca ggg gat tgg gtg gtc ctt gca 643
Leu Pro Met Val Ala Glu His Ala Ala Gly Asp Trp Val Val Leu Ala
170 175 180
ggt ttt cag tgg ttg gac aaa atc gac tat gtg atc gat tgc ttt aaa 691
Gly Phe Gln Trp Leu Asp Lys Ile Asp Tyr Val Ile Asp Cys Phe Lys
185 190 195
ccg gaa aat att gtg ctg gaa ttc ggt tca acc atg aca ccg gaa gac 739
Pro Glu Asn Ile Val Leu Glu Phe Gly Ser Thr Met Thr Pro Glu Asp
200 205 210
gcc gac cgc aat gaa tac ctc aga aga acg caa gcc aaa ttc cag ctt 787
Ala Asp Arg Asn Glu Tyr Leu Arg Arg Thr Gln Ala Lys Phe Gln Leu
215 220 225
cga ggc atc cta agc acc aac cca gaa tcc gct gcc cgg ggg agc gtg 835
Arg Gly Ile Leu Ser Thr Asn Pro Glu Ser Ala Ala Arg Gly Ser Val
230 235 240 245
cgg ctt gcc ggc gcc aag cag gca cta gcc cgc aag atg ccg ctt gcc 883
Arg Leu Ala Gly Ala Lys Gln Ala Leu Ala Arg Lys Met Pro Leu Ala
250 255 260
gac gcc gaa agc gag cta cat ccc atg ggc act acc tgg atg cgc agc 931
Asp Ala Glu Ser Glu Leu His Pro Met Gly Thr Thr Trp Met Arg Ser
265 270 275
ggg gat aca ttg tta aaa gca cac cct gat tac gcg gat ctc att gca 979
Gly Asp Thr Leu Leu Lys Ala His Pro Asp Tyr Ala Asp Leu Ile Ala
280 285 290
acc acg gtg gaa tta gct gct gaa tgt gct ttc acc cta gat ttg gtg 1027
Thr Thr Val Glu Leu Ala Ala Glu Cys Ala Phe Thr Leu Asp Leu Val
295 300 305
gcc ccg aat ctg ccc aag tgg gat acc cct ggt gaa cac acg gaa atg 1075
Ala Pro Asn Leu Pro Lys Trp Asp Thr Pro Gly Glu His Thr Glu Met
310 315 320 325
tcc tgg ctt gcg cac ctg gtt tcc act cgg att gat acc cgc tat gtg 1123
Ser Trp Leu Ala His Leu Val Ser Thr Arg Ile Asp Thr Arg Tyr Val
330 335 340
ggg cgc tcc gca gac atc aaa gca cga gct gcc aca caa att gac tat 1171
Gly Arg Ser Ala Asp Ile Lys Ala Arg Ala Ala Thr Gln Ile Asp Tyr
345 350 355
gaa tta ggc gtt att gaa aag ctg ggt ttt cca ggc tat ttc ctc gtc 1219
Glu Leu Gly Val Ile Glu Lys Leu Gly Phe Pro Gly Tyr Phe Leu Val
360 365 370
gtt aat gat ctg gtg gag ttt tgt cgc gat tcc aat att ttg tgc caa 1267
Val Asn Asp Leu Val Glu Phe Cys Arg Asp Ser Asn Ile Leu Cys Gln
375 380 385
ggc aga ggt tcc gcg gcg aac tcg gcg gtg tgc ttt gtc cta ggc atc 1315
Gly Arg Gly Ser Ala Ala Asn Ser Ala Val Cys Phe Val Leu Gly Ile
390 395 400 405
acc aac gcg gag ccg atc tct gct gga ttg ttg ttt gaa cgg ttt tta 1363
Thr Asn Ala Glu Pro Ile Ser Ala Gly Leu Leu Phe Glu Arg Phe Leu
410 415 420
tct cct gac cgg gat ggt cca cca gat att gac att gat att gaa tcc 1411
Ser Pro Asp Arg Asp Gly Pro Pro Asp Ile Asp Ile Asp Ile Glu Ser
425 430 435
ggc agg cgc gaa gaa gta atc caa tac gtg tat gaa aaa tac gga agg 1459
Gly Arg Arg Glu Glu Val Ile Gln Tyr Val Tyr Glu Lys Tyr Gly Arg
440 445 450
gat aac gca gct caa gta gcc aat gtc att acc tac cga aca aaa ggc 1507
Asp Asn Ala Ala Gln Val Ala Asn Val Ile Thr Tyr Arg Thr Lys Gly
455 460 465
gcg atg cgt gat gct gcc cgt gca ctg ggt tac ccg caa ggt gct gcc 1555
Ala Met Arg Asp Ala Ala Arg Ala Leu Gly Tyr Pro Gln Gly Ala Ala
470 475 480 485
gat gcc tgg gct aaa gga acc tcg gaa cca ccc gat gat gtg ctg gaa 1603
Asp Ala Trp Ala Lys Gly Thr Ser Glu Pro Pro Asp Asp Val Leu Glu
490 495 500
tta gct gcg caa ttt aaa ggg caa cca cgg cat ttg ggt att cac tcc 1651
Leu Ala Ala Gln Phe Lys Gly Gln Pro Arg His Leu Gly Ile His Ser
505 510 515
ggt ggc atg gtc att tgc gat cgc ccc atc gcc gat gtg gtg cca gtg 1699
Gly Gly Met Val Ile Cys Asp Arg Pro Ile Ala Asp Val Val Pro Val
520 525 530
gaa tgg gct cgg atg gat aac cgc tcg gtt gtg caa tgg gat aaa gat 1747
Glu Trp Ala Arg Met Asp Asn Arg Ser Val Val Gln Trp Asp Lys Asp
535 540 545
gac tgt gcc acg gca ggc ttg gtc aaa ttc gac ctt ttg gga ttg ggc 1795
Asp Cys Ala Thr Ala Gly Leu Val Lys Phe Asp Leu Leu Gly Leu Gly
550 555 560 565
atg ttg gaa gcc atc cat cac atg ctg gat ctg gtg gca gaa cac cga 1843
Met Leu Glu Ala Ile His His Met Leu Asp Leu Val Ala Glu His Arg
570 575 580
ggt aaa aag atc aat ttg tgg gaa cta gat ctg gcg gaa ccg gag gtc 1891
Gly Lys Lys Ile Asn Leu Trp Glu Leu Asp Leu Ala Glu Pro Glu Val
585 590 595
tat gac atg ttg tgc aag gca gat gcc gtg ggt gtg ttc cag gtg gaa 1939
Tyr Asp Met Leu Cys Lys Ala Asp Ala Val Gly Val Phe Gln Val Glu
600 605 610
tca cgt gcg cag tta tcc acg ctg cct cga ctc aag ccc cgc acc ttc 1987
Ser Arg Ala Gln Leu Ser Thr Leu Pro Arg Leu Lys Pro Arg Thr Phe
615 620 625
ttt gac ctg gtc gtg gag gta gct ctg att cgt cca ggt ccc atc caa 2035
Phe Asp Leu Val Val Glu Val Ala Leu Ile Arg Pro Gly Pro Ile Gln
630 635 640 645
ggc gga tcg gtg cac ccg tat ttg cgg cgc cgt gct ggt gaa gag gcc 2083
Gly Gly Ser Val His Pro Tyr Leu Arg Arg Arg Ala Gly Glu Glu Ala
650 655 660
atc act tat gac cac ccc gtg ttg gaa aag tct ttg ggt aaa acc tta 2131
Ile Thr Tyr Asp His Pro Val Leu Glu Lys Ser Leu Gly Lys Thr Leu
665 670 675
gga atc cca ctg ttt cag gaa cag ctc atg cag gta gct gtt gat gct 2179
Gly Ile Pro Leu Phe Gln Glu Gln Leu Met Gln Val Ala Val Asp Ala
680 685 690
gca ggt ttt agt ggt ggg gaa gcg gat tcc ttg cgc aga gcg atg ggg 2227
Ala Gly Phe Ser Gly Gly Glu Ala Asp Ser Leu Arg Arg Ala Met Gly
695 700 705
tcg aaa cgc tca cct gaa cgc atg gct gcg ttg cgc tcg cgg ttt ttc 2275
Ser Lys Arg Ser Pro Glu Arg Met Ala Ala Leu Arg Ser Arg Phe Phe
710 715 720 725
caa ggg ctg aaa gat acc aat ggg att gtg ggg gag acc gcc gag aaa 2323
Gln Gly Leu Lys Asp Thr Asn Gly Ile Val Gly Glu Thr Ala Glu Lys
730 735 740
ctg tgg aac aaa att gtg gcc ttt gct gcc tac ggt ttt ccg gaa tcg 2371
Leu Trp Asn Lys Ile Val Ala Phe Ala Ala Tyr Gly Phe Pro Glu Ser
745 750 755
cat tcg cag tcg ttt gcg tcc ttg gtg tat ttc tcc gcg tgg ttt aaa 2419
His Ser Gln Ser Phe Ala Ser Leu Val Tyr Phe Ser Ala Trp Phe Lys
760 765 770
tac cac tac ccg gct gaa ttc tgc gtg gga tta ttg cgg gca caa ccc 2467
Tyr His Tyr Pro Ala Glu Phe Cys Val Gly Leu Leu Arg Ala Gln Pro
775 780 785
atg ggt ttc tat tca cca cag tct ttg atc agt gat gcc aga cgc cac 2515
Met Gly Phe Tyr Ser Pro Gln Ser Leu Ile Ser Asp Ala Arg Arg His
790 795 800 805
ggc gtg agt atc ctg ccg atc acg gtc aat gat tcc ggt gtg gag gcc 2563
Gly Val Ser Ile Leu Pro Ile Thr Val Asn Asp Ser Gly Val Glu Ala
810 815 820
gat gct ccg aat ggt gcg att cga ttg ggg ctc aac ctg gtg aaa ggc 2611
Asp Ala Pro Asn Gly Ala Ile Arg Leu Gly Leu Asn Leu Val Lys Gly
825 830 835
ctt ggc cac gat gcc gcg caa aga ata gag gac aac gcc ccg ttt gat 2659
Leu Gly His Asp Ala Ala Gln Arg Ile Glu Asp Asn Ala Pro Phe Asp
840 845 850
tcc att ccg gat tta tcg cgc cgg gct gat ctt aat gtt gct caa gtt 2707
Ser Ile Pro Asp Leu Ser Arg Arg Ala Asp Leu Asn Val Ala Gln Val
855 860 865
gag gca ttg gcg cga gcg gga gcg gtg gac tgc ttg ggg gtc gga cgt 2755
Glu Ala Leu Ala Arg Ala Gly Ala Val Asp Cys Leu Gly Val Gly Arg
870 875 880 885
cga caa gca tta tgg caa gcg ggc gtc gca gcg acc gaa aaa cct gga 2803
Arg Gln Ala Leu Trp Gln Ala Gly Val Ala Ala Thr Glu Lys Pro Gly
890 895 900
atg ctg cct ggc ctt tcg gtg att gaa gct ccg gcg ttg ccg ggg atg 2851
Met Leu Pro Gly Leu Ser Val Ile Glu Ala Pro Ala Leu Pro Gly Met
905 910 915
agc gcc ttt gag ctg atg gcg acc aat att tcc gcc acg gga gtc acc 2899
Ser Ala Phe Glu Leu Met Ala Thr Asn Ile Ser Ala Thr Gly Val Thr
920 925 930
gcg gat tat cag ccg atg gcg ttg att cgg gag cgg atg gag gag ctg 2947
Ala Asp Tyr Gln Pro Met Ala Leu Ile Arg Glu Arg Met Glu Glu Leu
935 940 945
ggg atc gtg ccg gcg gat cgg cta ttg gag gtg gaa gat ggc acg cgg 2995
Gly Ile Val Pro Ala Asp Arg Leu Leu Glu Val Glu Asp Gly Thr Arg
950 955 960 965
ctg cgg atc gct ggc att gtc acg cac cgg cag cgc ccg caa act gcg 3043
Leu Arg Ile Ala Gly Ile Val Thr His Arg Gln Arg Pro Gln Thr Ala
970 975 980
tcg ggg ctg aca ttt tta ggg atg gag gat gag acc ggg ctg atg aat 3091
Ser Gly Leu Thr Phe Leu Gly Met Glu Asp Glu Thr Gly Leu Met Asn
985 990 995
gtg atg gtg tcc gtt ggg ttg tgg cag cgg cag cgc gtg ctg gcc aga 3139
Val Met Val Ser Val Gly Leu Trp Gln Arg Gln Arg Val Leu Ala Arg
1000 1005 1010
aat gcc aag gcg ttg att att cga ggg att gtg cag aat gcg caa ggg 3187
Asn Ala Lys Ala Leu Ile Ile Arg Gly Ile Val Gln Asn Ala Gln Gly
1015 1020 1025
gtg gcg aca gtt gtc gct gac cgg ttg gaa ccg ttg gac atg ggg gag 3235
Val Ala Thr Val Val Ala Asp Arg Leu Glu Pro Leu Asp Met Gly Glu
1030 1035 1040 1045
ttt ctc agc cgt ggc tca cga gat ttt cga taatttggca aagtggacat 3285
Phe Leu Ser Arg Gly Ser Arg Asp Phe Arg
1050 1055
tatgacaaat 3295
<210>14
<211>1055
<212>PRT
<213>谷氨酸棒状杆菌
<400>14
Met Tyr Pro Ile Ile Met Glu Trp Asn Gly Gly Gly Ser Phe Asn Gly
1 5 10 15
Arg Pro Leu Ser Trp Ser Lys Leu Glu Arg Ile Leu Ser Gly Lys Lys
20 25 30
Val Glu Ser Leu Arg Pro Val Leu His Glu Pro Asp Ala Gln Ala Pro
35 40 45
Ser Ser Ala Met Gln Gly Glu Ala Ser Val Pro Phe Ala Glu Leu His
50 55 60
Ala Thr Ser Ser Tyr Asn Phe Leu Thr Gly Ala Ser Asp Pro Ser Asp
65 70 75 80
Val Val Val Gln Ala Lys Lys Leu Gly Leu Val Ala Leu Ser Val Met
85 90 95
Asp Arg Asp Gly Phe Tyr Gly Ala Val Arg Phe Ala Glu Ala Ala Ala
100 105 110
Glu Ala Gly Met His Thr Val Tyr Gly Ala Glu Leu Ser Leu Gln Glu
115 120 125
Gly Val Leu Thr Val Leu Cys Lys Asn Pro Glu Gly Tyr Lys Lys Leu
130 135 140
Ser His Leu Ile Ser Asp Ala Lys Met Ala Thr Gly Glu Lys Gly Glu
145 150 155 160
Val Arg Tyr Pro Pro Leu Pro Met Val Ala Glu His Ala Ala Gly Asp
165 170 175
Trp Val Val Leu Ala Gly Phe Gln Trp Leu Asp Lys Ile Asp Tyr Val
180 185 190
Ile Asp Cys Phe Lys Pro Glu Asn Ile Val Leu Glu Phe Gly Ser Thr
195 200 205
Met Thr Pro Glu Asp Ala Asp Arg Asn Glu Tyr Leu Arg Arg Thr Gln
210 215 220
Ala Lys Phe Gln Leu Arg Gly Ile Leu Ser Thr Asn Pro Glu Ser Ala
225 230 235 240
Ala Arg Gly Ser Val Arg Leu Ala Gly Ala Lys Gln Ala Leu Ala Arg
245 250 255
Lys Met Pro Leu Ala Asp Ala Glu Ser Glu Leu His Pro Met Gly Thr
260 265 270
Thr Trp Met Arg Ser Gly Asp Thr Leu Leu Lys Ala His Pro Asp Tyr
275 280 285
Ala Asp Leu Ile Ala Thr Thr Val Glu Leu Ala Ala Glu Cys Ala Phe
290 295 300
Thr Leu Asp Leu Val Ala Pro Asn Leu Pro Lys Trp Asp Thr Pro Gly
305 310 315 320
Glu His Thr Glu Met Ser Trp Leu Ala His Leu Val Ser Thr Arg Ile
325 330 335
Asp Thr Arg Tyr Val Gly Arg Ser Ala Asp Ile Lys Ala Arg Ala Ala
340 345 350
Thr Gln Ile Asp Tyr Glu Leu Gly Val Ile Glu Lys Leu Gly Phe Pro
355 360 365
Gly Tyr Phe Leu Val Val Asn Asp Leu Val Glu Phe Cys Arg Asp Ser
370 375 380
Asn Ile Leu Cys Gln Gly Arg Gly Ser Ala Ala Asn Ser Ala Val Cys
385 390 395 400
Phe Val Leu Gly Ile Thr Asn Ala Glu Pro Ile Ser Ala Gly Leu Leu
405 410 415
Phe Glu Arg Phe Leu Ser Pro Asp Arg Asp Gly Pro Pro Asp Ile Asp
420 425 430
Ile Asp Ile Glu Ser Gly Arg Arg Glu Glu Val Ile Gln Tyr Val Tyr
435 440 445
Glu Lys Tyr Gly Arg Asp Asn Ala Ala Gln Val Ala Asn Val Ile Thr
450 455 460
Tyr Arg Thr Lys Gly Ala Met Arg Asp Ala Ala Arg Ala Leu Gly Tyr
465 470 475 480
Pro Gln Gly Ala Ala Asp Ala Trp Ala Lys Gly Thr Ser Glu Pro Pro
485 490 495
Asp Asp Val Leu Glu Leu Ala Ala Gln Phe Lys Gly Gln Pro Arg His
500 505 510
Leu Gly Ile His Ser Gly Gly Met Val Ile Cys Asp Arg Pro Ile Ala
515 520 525
Asp Val Val Pro Val Glu Trp Ala Arg Met Asp Asn Arg Ser Val Val
530 535 540
Gln Trp Asp Lys Asp Asp Cys Ala Thr Ala Gly Leu Val Lys Phe Asp
545 550 555 560
Leu Leu Gly Leu Gly Met Leu Glu Ala Ile His His Met Leu Asp Leu
565 570 575
Val Ala Glu His Arg Gly Lys Lys Ile Asn Leu Trp Glu Leu Asp Leu
580 585 590
Ala Glu Pro Glu Val Tyr Asp Met Leu Cys Lys Ala Asp Ala Val Gly
595 600 605
Val Phe Gln Val Glu Ser Arg Ala Gln Leu Ser Thr Leu Pro Arg Leu
610 615 620
Lys Pro Arg Thr Phe Phe Asp Leu Val Val Glu Val Ala Leu Ile Arg
625 630 635 640
Pro Gly Pro Ile Gln Gly Gly Ser Val His Pro Tyr Leu Arg Arg Arg
645 650 655
Ala Gly Glu Glu Ala Ile Thr Tyr Asp His Pro Val Leu Glu Lys Ser
660 665 670
Leu Gly Lys Thr Leu Gly Ile Pro Leu Phe Gln Glu Gln Leu Met Gln
675 680 685
Val Ala Val Asp Ala Ala Gly Phe Ser Gly Gly Glu Ala Asp Ser Leu
690 695 700
Arg Arg Ala Met Gly Ser Lys Arg Ser Pro Glu Arg Met Ala Ala Leu
705 710 715 720
Arg Ser Arg Phe Phe Gln Gly Leu Lys Asp Thr Asn Gly Ile Val Gly
725 730 735
Glu Thr Ala Glu Lys Leu Trp Asn Lys Ile Val Ala Phe Ala Ala Tyr
740 745 750
Gly Phe Pro Glu Ser His Ser Gln Ser Phe Ala Ser Leu Val Tyr Phe
755 760 765
Ser Ala Trp Phe Lys Tyr His Tyr Pro Ala Glu Phe Cys Val Gly Leu
770 775 780
Leu Arg Ala Gln Pro Met Gly Phe Tyr Ser Pro Gln Ser Leu Ile Ser
785 790 795 800
Asp Ala Arg Arg His Gly Val Ser Ile Leu Pro Ile Thr Val Asn Asp
805 810 815
Ser Gly Val Glu Ala Asp Ala Pro Asn Gly Ala Ile Arg Leu Gly Leu
820 825 830
Asn Leu Val Lys Gly Leu Gly His Asp Ala Ala Gln Arg Ile Glu Asp
835 840 845
Asn Ala Pro Phe Asp Ser Ile Pro Asp Leu Ser Arg Arg Ala Asp Leu
850 855 860
Asn Val Ala Gln Val Glu Ala Leu Ala Arg Ala Gly Ala Val Asp Cys
865 870 875 880
Leu Gly Val Gly Arg Arg Gln Ala Leu Trp Gln Ala Gly Val Ala Ala
885 890 895
Thr Glu Lys Pro Gly Met Leu Pro Gly Leu Ser Val Ile Glu Ala Pro
900 905 910
Ala Leu Pro Gly Met Ser Ala Phe Glu Leu Met Ala Thr Asn Ile Ser
915 920 925
Ala Thr Gly Val Thr Ala Asp Tyr Gln Pro Met Ala Leu Ile Arg Glu
930 935 940
Arg Met Glu Glu Leu Gly Ile Val Pro Ala Asp Arg Leu Leu Glu Val
945 950 955 960
Glu Asp Gly Thr Arg Leu Arg Ile Ala Gly Ile Val Thr His Arg Gln
965 970 975
Arg Pro Gln Thr Ala Ser Gly Leu Thr Phe Leu Gly Met Glu Asp Glu
980 985 990
Thr Gly Leu Met Asn Val Met Val Ser Val Gly Leu Trp Gln Arg Gln
995 1000 1005
Arg Val Leu Ala Arg Asn Ala Lys Ala Leu Ile Ile Arg Gly Ile Val
1010 1015 1020
Gln Asn Ala Gln Gly Val Ala Thr Val Val Ala Asp Arg Leu Glu Pro
1025 1030 1035 1040
Leu Asp Met Gly Glu Phe Leu Ser Arg Gly Ser Arg Asp Phe Arg
1045 1050 1055
<210>15
<211>526
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(496)
<223>RXA00694
<400>15
cgactattga gttcggtgag cgttgagtgg ccagaaaaca cacggttaac gtccaaggtg 60
ggaagggaac cccaacgaga aaggcatcag gtcgtctcta atg aca atg act gat 115
Met Thr Met Thr Asp
1 5
cca atc gcc gac atg ctg tcg cgc gtg cgc aat gct agc aat gcg cac 163
Pro Ile Ala Asp Met Leu Ser Arg Val Arg Asn Ala Ser Asn Ala His
10 15 20
cac gac acc gtg tcc atg cca tcc tcc aag atc aag gca aac atc gcc 211
His Asp Thr Val Ser Met Pro Ser Ser Lys Ile Lys Ala Asn Ile Ala
25 30 35
gag atc ttg aag cag gaa ggc tac atc gct aac tac acc gtt gag gat 259
Glu Ile Leu Lys Gln Glu Gly Tyr Ile Ala Asn Tyr Thr Val Glu Asp
40 45 50
gca aag gtc ggc aag acc ctg tcc ctc gag ctg aag tac agc aac acc 307
Ala Lys Val Gly Lys Thr Leu Ser Leu Glu Leu Lys Tyr Ser Asn Thr
55 60 65
cgt gag cgc tcc atc gct ggt ctg cgc cgc gtt tcc aag cct ggt ctg 355
Arg Glu Arg Ser Ile Ala Gly Leu Arg Arg Val Ser Lys Pro Gly Leu
70 75 80 85
cgt gta tac gct aag tcc acc aat ctg cca cag gtt ctg ggc ggc ctt 403
Arg Val Tyr Ala Lys Ser Thr Asn Leu Pro Gln Val Leu Gly Gly Leu
90 95 100
ggc gtg gct atc att tcc acg tca cag ggc ctc ctg acc gac cgt cag 451
Gly Val Ala Ile Ile Ser Thr Ser Gln Gly Leu Leu Thr Asp Arg Gln
105 110 115
gct acc gag aag ggc gta ggc gga gaa gtc ctc gcc tac gtc tgg 496
Ala Thr Glu Lys Gly Val Gly Gly Glu Val Leu Ala Tyr Val Trp
120 125 130
taatagggag gattgactaa atatgtcacg 526
<210>16
<211>132
<212>PRT
<213>谷氨酸棒状杆菌
<400>16
Met Thr Met Thr Asp Pro Ile Ala Asp Met Leu Ser Arg Val Arg Asn
1 5 10 15
Ala Ser Asn Ala His His Asp Thr Val Ser Met Pro Ser Ser Lys Ile
20 25 30
Lys Ala Asn Ile Ala Glu Ile Leu Lys Gln Glu Gly Tyr Ile Ala Asn
35 40 45
Tyr Thr Val Glu Asp Ala Lys Val Gly Lys Thr Leu Ser Leu Glu Leu
50 55 60
Lys Tyr Ser Asn Thr Arg Glu Arg Ser Ile Ala Gly Leu Arg Arg Val
65 70 75 80
Ser Lys Pro Gly Leu Arg Val Tyr Ala Lys Ser Thr Asn Leu Pro Gln
85 90 95
Val Leu Gly Gly Leu Gly Val Ala Ile Ile Ser Thr Ser Gln Gly Leu
100 105 110
Leu Thr Asp Arg Gln Ala Thr Glu Lys Gly Val Gly Gly Glu Val Leu
115 120 125
Ala Tyr Val Trp
130
<210>17
<211>532
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(502)
<223>RXA00696
<400>17
gatccgccgc ctgcgtaagg acgatcctta caagggtaag ggcatccgct acgagggtga 60
gcagatccgc cgcaaggtcg gaaagacggg taagtaagca atg agc aac act gaa 115
Met Ser Asn Thr Glu
1 5
aac aag cag aag cgc gtt tcc gtt ggc aag gac atc gcg act cgt cgt 163
Asn Lys Gln Lys Arg Val Ser Val Gly Lys Asp Ile Ala Thr Arg Arg
10 15 20
cgc gtt gcc cgt gca cgc cgc cac ttc cgc atc cgc aag aac ctg cgt 211
Arg Val Ala Arg Ala Arg Arg His Phe Arg Ile Arg Lys Asn Leu Arg
25 30 35
ggc acc cca gag gct cca cgt ttg gtt gtc cac cgc tct tct cgc cac 259
Gly Thr Pro Glu Ala Pro Arg Leu Val Val His Arg Ser Ser Arg His
40 45 50
atg cac gtt cag atc atc gat gac gtt gca ggc cac acc ctg gct gca 307
Met His Val Gln Ile Ile Asp Asp Val Ala Gly His Thr Leu Ala Ala
55 60 65
gct tct tcc atc gag gct gag gtt cgc gca act gag ggc gac aag aag 355
Ala Ser Ser Ile Glu Ala Glu Val Arg Ala Thr Glu Gly Asp Lys Lys
70 75 80 85
gct aag ggc gca aag gtc ggt cag ctg atc gcc gag cgc gct aag gct 403
Ala Lys Gly Ala Lys Val Gly Gln Leu Ile Ala Glu Arg Ala Lys Ala
90 95 100
gct ggt atc gag cag gtc gtc ttc gac cgc gct ggt tac aag tac cac 451
Ala Gly Ile Glu Gln Val Val Phe Asp Arg Ala Gly Tyr Lys Tyr His
105 110 115
ggc cgc gtt gca gct ctc gct gac gcc gct cgt gaa ggt ggt ctg aaa 499
Gly Arg Val Ala Ala Leu Ala Asp Ala Ala Arg Glu Gly Gly Leu Lys
120 125 130
ttc taatgatgac catttctaag aacatcaacg 532
Phe
<210>18
<211>134
<212>PRT
<213>谷氨酸棒状杆菌
<400>18
Met Ser Asn Thr Glu Asn Lys Gln Lys Arg Val Ser Val Gly Lys Asp
1 5 10 15
Ile Ala Thr Arg Arg Arg Val Ala Arg Ala Arg Arg His Phe Arg Ile
20 25 30
Arg Lys Asn Leu Arg Gly Thr Pro Glu Ala Pro Arg Leu Val Val His
35 40 45
Arg Ser Ser Arg His Met His Val Gln Ile Ile Asp Asp Val Ala Gly
50 55 60
His Thr Leu Ala Ala Ala Ser Ser Ile Glu Ala Glu Val Arg Ala Thr
65 70 75 80
Glu Gly Asp Lys Lys Ala Lys Gly Ala Lys Val Gly Gln Leu Ile Ala
85 90 95
Glu Arg Ala Lys Ala Ala Gly Ile Glu Gln Val Val Phe Asp Arg Ala
100 105 110
Gly Tyr Lys Tyr His Gly Arg Val Ala Ala Leu Ala Asp Ala Ala Arg
115 120 125
Glu Gly Gly Leu Lys Phe
130
<210>19
<211>1372
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1342)
<223>RXA00807
<400>19
tgctctaagc aatcgactca tgctcaatac tctaacgtgc cggtgcgaca tcgcgaggat 60
gtagtgagga agtagtgtag agatcgtgac taattcgagt gtg ttt gac agt ctc 115
Val Phe Asp Ser Leu
1 5
gcc ggc tcc aaa aca gtg tcc aag acg ctt ttc gac gcg gcc tcc agc 163
Ala Gly Ser Lys Thr Val Ser Lys Thr Leu Phe Asp Ala Ala Ser Ser
10 15 20
gcg cgt gcc ctt gtc cgc gcc cga acc aca gaa cgt gcc cgc gcc cgg 211
Ala Arg Ala Leu Val Arg Ala Arg Thr Thr Glu Arg Ala Arg Ala Arg
25 30 35
gca gaa cac caa aac cct gca atg atc cac gac tcc ggc ttt gcc cag 259
Ala Glu His Gln Asn Pro Ala Met Ile His Asp Ser Gly Phe Ala Gln
40 45 50
tca tgg ctg ttt aca ggc cct ccc gga tcg gga cgt tct gtg gca gcc 307
Ser Trp Leu Phe Thr Gly Pro Pro Gly Ser Gly Arg Ser Val Ala Ala
55 60 65
aag gtt ttc gcc gct acg ctc gta tgt tcg aat ccg gat gtt gtg ggc 355
Lys Val Phe Ala Ala Thr Leu Val Cys Ser Asn Pro Asp Val Val Gly
70 75 80 85
tgt gga caa tgc gag gat tgc cgc gcc gcc atg gga ggc agc cac ccc 403
Cys Gly Gln Cys Glu Asp Cys Arg Ala Ala Met Gly Gly Ser His Pro
90 95 100
gat att gaa cac atc gtc ccg cag caa ttg tct atc ggt gtt gat gca 451
Asp Ile Glu His Ile Val Pro Gln Gln Leu Ser Ile Gly Val Asp Ala
105 110 115
gct aga gag gtc atc aaa gcc gca gcg gtc agt cct gtt gca gga aac 499
Ala Arg Glu Val Ile Lys Ala Ala Ala Val Ser Pro Val Ala Gly Asn
120 125 130
tgg cga gtc gtc atc ttc gaa aac gcc gac cga ctc acc atg caa gcc 547
Trp Arg Val Val Ile Phe Glu Asn Ala Asp Arg Leu Thr Met Gln Ala
135 140 145
gcc aac gcc ttg ctg aaa acc gtg gag gaa cca acc gaa agc acc gtg 595
Ala Asn Ala Leu Leu Lys Thr Val Glu Glu Pro Thr Glu Ser Thr Val
150 155 160 165
atg att ctg tgc gca ccc acc aca gac ccc cgc gac att gcg atc acc 643
Met Ile Leu Cys Ala Pro Thr Thr Asp Pro Arg Asp Ile Ala Ile Thr
170 175 180
ctc cgc tcc cgc tgc agg cac ctc tac att ccc acc ccc tcc atc gcg 691
Leu Arg Ser Arg Cys Arg His Leu Tyr Ile Pro Thr Pro Ser Ile Ala
185 190 195
gaa gtc gca cga atc ctc gta gct gaa ggc aac gtc agc caa gcg gat 739
Glu Val Ala Arg Ile Leu Val Ala Glu Gly Asn Val Ser Gln Ala Asp
200 205 210
gca gaa tta gcg gcg gct gcc tca ggc gct cac atc ggc agg gct cga 787
Ala Glu Leu Ala Ala Ala Ala Ser Gly Ala His Ile Gly Arg Ala Arg
215 220 225
tac tta gcg cac aac aac gcc gcc caa cgc aga cgc gcc agc atc ctc 835
Tyr Leu Ala His Asn Asn Ala Ala Gln Arg Arg Arg Ala Ser Ile Leu
230 235 240 245
aac ctg gcc gaa tta atc ttc cac ggt gat gtc gcc ttc cgc tcc gta 883
Asn Leu Ala Glu Leu Ile Phe His Gly Asp Val Ala Phe Arg Ser Val
250 255 260
aac acc ttg gtc aaa atg gtg gaa acc gaa gcc aaa gac agc aac aaa 931
Asn Thr Leu Val Lys Met Val Glu Thr Glu Ala Lys Asp Ser Asn Lys
265 270 275
gaa aaa gaa gaa gcc gaa ctc gaa gcc gta aga atc tca ctc ggc atg 979
Glu Lys Glu Glu Ala Glu Leu Glu Ala Val Arg Ile Ser Leu Gly Met
280 285 290
ggg gcc aaa ggc aaa ggt gtc cac aaa gca gta cgt gga gga gcg gga 1027
Gly Ala Lys Gly Lys Gly Val His Lys Ala Val Arg Gly Gly Ala Gly
295 300 305
gat ttc aaa gca ctc gaa gac caa caa aaa ctc cga cgc acc cga ttc 1075
Asp Phe Lys Ala Leu Glu Asp Gln Gln Lys Leu Arg Arg Thr Arg Phe
310 315 320 325
ctc cgc gac agc ctc gac ctc gca ctc gtc gac cta gcc ggc atc tac 1123
Leu Arg Asp Ser Leu Asp Leu Ala Leu Val Asp Leu Ala Gly Ile Tyr
330 335 340
cgc gat gcc atc atc att tcc tcc caa gcc caa gtc gga ctc aca cac 1171
Arg Asp Ala Ile Ile Ile Ser Ser Gln Ala Gln Val Gly Leu Thr His
345 350 355
ccc gat atg gaa ggc ctc tcc caa gaa ctc gca aca aaa gta agc caa 1219
Pro Asp Met Glu Gly Leu Ser Gln Glu Leu Ala Thr Lys Val Ser Gln
360 365 370
gaa gga ctc ctg gca tgc ctc gac gcg atc tcc aaa tgc cgc gaa tcc 1267
Glu Gly Leu Leu Ala Cys Leu Asp Ala Ile Ser Lys Cys Arg Glu Ser
375 380 385
ttc ggc ttc aat gta cga ccc atc gtg gcc atg gac gcc ctg gta gga 1315
Phe Gly Phe Asn Val Arg Pro Ile Val Ala Met Asp Ala Leu Val Gly
390 395 400 405
cgc ctg cgc aaa gcc tac aaa gtg tcc taaacacccc aaattattga 1362
Arg Leu Arg Lys Ala Tyr Lys Val Ser
410
agtcaagaac 1372
<210>20
<211>414
<212>PRT
<213>谷氨酸棒状杆菌
<400>20
Val Phe Asp Ser Leu Ala Gly Ser Lys Thr Val Ser Lys Thr Leu Phe
1 5 10 15
Asp Ala Ala Ser Ser Ala Arg Ala Leu Val Arg Ala Arg Thr Thr Glu
20 25 30
Arg Ala Arg Ala Arg Ala Glu His Gln Asn Pro Ala Met Ile His Asp
35 40 45
Ser Gly Phe Ala Gln Ser Trp Leu Phe Thr Gly Pro Pro Gly Ser Gly
50 55 60
Arg Ser Val Ala Ala Lys Val Phe Ala Ala Thr Leu Val Cys Ser Asn
65 70 75 80
Pro Asp Val Val Gly Cys Gly Gln Cys Glu Asp Cys Arg Ala Ala Met
85 90 95
Gly Gly Ser His Pro Asp Ile Glu His Ile Val Pro Gln Gln Leu Ser
100 105 110
Ile Gly Val Asp Ala Ala Arg Glu Val Ile Lys Ala Ala Ala Val Ser
115 120 125
Pro Val Ala Gly Asn Trp Arg Val Val Ile Phe Glu Agn Ala Asp Arg
130 135 140
Leu Thr Met Gln Ala Ala Asn Ala Leu Leu Lys Thr Val Glu Glu Pro
145 150 155 160
Thr Glu Ser Thr Val Met Ile Leu Cys Ala Pro Thr Thr Asp Pro Arg
165 170 175
Asp Ile Ala Ile Thr Leu Arg Ser Arg Cys Arg His Leu Tyr Ile Pro
180 185 190
Thr Pro Ser Ile Ala Glu Val Ala Arg Ile Leu Val Ala Glu Gly Asn
195 200 205
Val Ser Gln Ala Asp Ala Glu Leu Ala Ala Ala Ala Ser Gly Ala His
210 215 220
Ile Gly Arg Ala Arg Tyr Leu Ala His Asn Asn Ala Ala Gln Arg Arg
225 230 235 240
Arg Ala Ser Ile Leu Asn Leu Ala Glu Leu Ile Phe His Gly Asp Val
245 250 255
Ala Phe Arg Ser Val Asn Thr Leu Val Lys Met Val Glu Thr Glu Ala
260 265 270
Lys Asp Ser Asn Lys Glu Lys Glu Glu Ala Glu Leu Glu Ala Val Arg
275 280 285
Ile Ser Leu Gly Met Gly Ala Lys Gly Lys Gly Val His Lys Ala Val
290 295 300
Arg Gly Gly Ala Gly Asp Phe Lys Ala Leu Glu Asp Gln Gln Lys Leu
305 310 315 320
Arg Arg Thr Arg Phe Leu Arg Asp Ser Leu Asp Leu Ala Leu Val Asp
325 330 335
Leu Ala Gly Ile Tyr Arg Asp Ala Ile Ile Ile Ser Ser Gln Ala Gln
340 345 350
Val Gly Leu Thr His Pro Asp Met Glu Gly Leu Ser Gln Glu Leu Ala
355 360 365
Thr Lys Val Ser Gln Glu Gly Leu Leu Ala Cys Leu Asp Ala Ile Ser
370 375 380
Lys Cys Arg Glu Ser Phe Gly Phe Asn Val Arg Pro Ile Val Ala Met
385 390 395 400
Asp Ala Leu Val Gly Arg Leu Arg Lys Ala Tyr Lys Val Ser
405 410
<210>21
<211>1531
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1501)
<223>RXA01238
<400>21
ccacgtccag aatcttcttg aattccccgg cgcgcttaaa tgatgtccac cccacctagt 60
agggtggtgt ccattgattc agatcgaaag gaccacacct gtg atc gcg gct tac 115
Val Ile Ala Ala Tyr
1 5
ggc gca tcc atc tct ttg gat gat tcc acc ctc acc atc tct tat tcc 163
Gly Ala Ser Ile Ser Leu Asp Asp Ser Thr Leu Thr Ile Ser Tyr Ser
10 15 20
cct ctt ctt gct gct ctt tct aag tcc agc gca caa tcg gaa tcg gtt 211
Pro Leu Leu Ala Ala Leu Ser Lys Ser Ser Ala Gln Ser Glu Ser Val
25 30 35
gat ctg aca cag gtc tct gga gta tct gtg cag gat ccc act gct ttt 259
Asp Leu Thr Gln Val Ser Gly Val Ser Val Gln Asp Pro Thr Ala Phe
40 45 50
act cac ggc ttt tta aac ctg gag ggc gtg gat aaa tcc atc gcc ttt 307
Thr His Gly Phe Leu Asn Leu Glu Gly Val Asp Lys Ser Ile Ala Phe
55 60 65
gcc cca aat agt tcg gca gat tta gct gcg ctg gca gct gat att gat 355
Ala Pro Asn Ser Ser Ala Asp Leu Ala Ala Leu Ala Ala Asp Ile Asp
70 75 80 85
gct gtg ttg aag ggt gaa aag cca cag cac ctg ggt ggc gga gcc cca 403
Ala Val Leu Lys Gly Glu Lys Pro Gln His Leu Gly Gly Gly Ala Pro
90 95 100
gta gtg cct tca gct cca tct act gtt gct ggt ctg aat ttc gtg ggc 451
Val Val Pro Ser Ala Pro Ser Thr Val Ala Gly Leu Asn Phe Val Gly
105 110 115
ttt gac gta gag acg gcc aat gat gat tgg ggt tcc atc tgc cag att 499
Phe Asp Val Glu Thr Ala Asn Asp Asp Trp Gly Ser Ile Cys Gln Ile
120 125 130
ggt ttg gtc aag tac gtc gat ggt gtg gag gaa tct tcg gag tca tgg 547
Gly Leu Val Lys Tyr Val Asp Gly Val Glu Glu Ser Ser Glu Ser Trp
135 140 145
ttg tgt act cct cct gag agc ctg aat ttc ttc aat gag atc aac att 595
Leu Cys Thr Pro Pro Glu Ser Leu Asn Phe Phe Asn Glu Ile Asn Ile
150 155 160 165
ggt att cac ggc atc acc cca gag atg gtt gct gat cag cct cgt ttt 643
Gly Ile His Gly Ile Thr Pro Glu Met Val Ala Asp Gln Pro Arg Phe
170 175 180
gca gac ctt gtg ccc aag atg gtg gag ttc gtt ggg gat ttg ccg ttg 691
Ala Asp Leu Val Pro Lys Met Val Glu Phe Val Gly Asp Leu Pro Leu
185 190 195
gtt gct cac aat gcg cag ttt gat ttc acc gca ttg tcg cgc gcg tgt 739
Val Ala His Asn Ala Gln Phe Asp Phe Thr Ala Leu Ser Arg Ala Cys
200 205 210
gct gcc tca ggg atc gat gtc cca gag atg att tat ggc tgc tcg ttg 787
Ala Ala Ser Gly Ile Asp Val Pro Glu Met Ile Tyr Gly Cys Ser Leu
215 220 225
acg ttg gca cgc aat gag aag ctg cag gtg gaa aac cat aag ctt cca 835
Thr Leu Ala Arg Asn Glu Lys Leu Gln Val Glu Asn His Lys Leu Pro
230 235 240 245
acg gtg gct agt cat tta ggg ttt gag ctg aaa aac cac cac gat gct 883
Thr Val Ala Ser His Leu Gly Phe Glu Leu Lys Asn His His Asp Ala
250 255 260
gct gaa gat gct cgc gcg tgt gct gcg att acc att gcg ttg gca aag 931
Ala Glu Asp Ala Arg Ala Cys Ala Ala Ile Thr Ile Ala Leu Ala Lys
265 270 275
cgc cac agc ttt gag ggc agc ttt gtg gat ttc gtt cac agc cgt ggt 979
Arg His Ser Phe Glu Gly Ser Phe Val Asp Phe Val His Ser Arg Gly
280 285 290
ttc acc atg gga acc gtg gat aac gcc cgg gtg tat ccg gtg ctg aag 1027
Phe Thr Met Gly Thr Val Asp Asn Ala Arg Val Tyr Pro Val Leu Lys
295 300 305
gat cgt tct gga gct aac gtt gcg ttg cag cgt cga aac ttt ggt ttg 1075
Asp Arg Ser Gly Ala Asn Val Ala Leu Gln Arg Arg Asn Phe Gly Leu
310 315 320 325
gat gca ggc aag acc gaa gtc ccc gtg cag cca gct gtt gat cca gcg 1123
Asp Ala Gly Lys Thr Glu Val Pro Val Gln Pro Ala Val Asp Pro Ala
330 335 340
tgg gaa acc ccg aag gcg gag cca aaa aag caa tct ggc cgc cgt gca 1171
Trp Glu Thr Pro Lys Ala Glu Pro Lys Lys Gln Ser Gly Arg Arg Ala
345 350 355
ccg tgg gac aag gtg gct acc cct gag gtc att cca gat ccc aat ccc 1219
Pro Trp Asp Lys Val Ala Thr Pro Glu Val Ile Pro Asp Pro Asn Pro
360 365 370
gat gct gat ccg tcg agc att ctc tat ggc cag aat gtg acc ttg act 1267
Asp Ala Asp Pro Ser Ser Ile Leu Tyr Gly Gln Asn Val Thr Leu Thr
375 380 385
ggt gat ttc gag cca tat gaa aag ggt gcg ctg tgg cag cgg atc gct 1315
Gly Asp Phe Glu Pro Tyr Glu Lys Gly Ala Leu Trp Gln Arg Ile Ala
390 395 400 405
gat caa ggt gcg ctg atc ggt aag aac gtg act aag aag acc acc att 1363
Asp Gln Gly Ala Leu Ile Gly Lys Asn Val Thr Lys Lys Thr Thr Ile
410 415 420
ttg gtt gct ggc ccg tgg gca acg att acc agt aag caa aaa cgt gct 1411
Leu Val Ala Gly Pro Trp Ala Thr Ile Thr Ser Lys Gln Lys Arg Ala
425 430 435
gag gag ttg aaa gaa aaa gga cag gac atc cag atc tgg gat gaa aag 1459
Glu Glu Leu Lys Glu Lys Gly Gln Asp Ile Gln Ile Trp Asp Glu Lys
440 445 450
cag ctg ttt acg gct ctg ggg tta gat gaa cag ccc ccg ttt 1501
Gln Leu Phe Thr Ala Leu Gly Leu Asp Glu Gln Pro Pro Phe
455 460 465
taaaagaatt ttctaaaact cgggaacctt 1531
<210>22
<211>467
<212>PRT
<213>谷氨酸棒状杆菌
<400>22
Val Ile Ala Ala Tyr Gly Ala Ser Ile Ser Leu Asp Asp Ser Thr Leu
1 5 10 15
Thr Ile Ser Tyr Ser Pro Leu Leu Ala Ala Leu Ser Lys Ser Ser Ala
20 25 30
Gln Ser Glu Ser Val Asp Leu Thr Gln Val Ser Gly Val Ser Val Gln
35 40 45
Asp Pro Thr Ala Phe Thr His Gly Phe Leu Asn Leu Glu Gly Val Asp
50 55 60
Lys Ser Ile Ala Phe Ala Pro Asn Ser Ser Ala Asp Leu Ala Ala Leu
65 70 75 80
Ala Ala Asp Ile Asp Ala Val Leu Lys Gly Glu Lys Pro Gln His Leu
85 90 95
Gly Gly Gly Ala Pro Val Val Pro Ser Ala Pro Ser Thr Val Ala Gly
100 105 110
Leu Asn Phe Val Gly Phe Asp Val Glu Thr Ala Asn Asp Asp Trp Gly
115 120 125
Ser Ile Cys Gln Ile Gly Leu Val Lys Tyr Val Asp Gly Val Glu Glu
130 135 140
Ser Ser Glu Ser Trp Leu Cys Thr Pro Pro Glu Ser Leu Asn Phe Phe
145 150 155 160
Asn Glu Ile Asn Ile Gly Ile His Gly Ile Thr Pro Glu Met Val Ala
165 170 175
Asp Gln Pro Arg Phe Ala Asp Leu Val Pro Lys Met Val Glu Phe Val
180 185 190
Gly Asp Leu Pro Leu Val Ala His Asn Ala Gln Phe Asp Phe Thr Ala
195 200 205
Leu Ser Arg Ala Cys Ala Ala Ser Gly Ile Asp Val Pro Glu Met Ile
210 215 220
Tyr Gly Cys Ser Leu Thr Leu Ala Arg Asn Glu Lys Leu Gln Val Glu
225 230 235 240
Asn His Lys Leu Pro Thr Val Ala Ser His Leu Gly Phe Glu Leu Lys
245 250 255
Asn His His Asp Ala Ala Glu Asp Ala Arg Ala Cys Ala Ala Ile Thr
260 265 270
Ile Ala Leu Ala Lys Arg His Ser Phe Glu Gly Ser Phe Val Asp Phe
275 280 285
Val His Ser Arg Gly Phe Thr Met Gly Thr Val Asp Asn Ala Arg Val
290 295 300
Tyr Pro Val Leu Lys Asp Arg Ser Gly Ala Asn Val Ala Leu Gln Arg
305 310 315 320
Arg Asn Phe Gly Leu Asp Ala Gly Lys Thr Glu Val Pro Val Gln Pro
325 330 335
Ala Val Asp Pro Ala Trp Glu Thr Pro Lys Ala Glu Pro Lys Lys Gln
340 345 350
Ser Gly Arg Arg Ala Pro Trp Asp Lys Val Ala Thr Pro Glu Val Ile
355 360 365
Pro Asp Pro Asn Pro Asp Ala Asp Pro Ser Ser Ile Leu Tyr Gly Gln
370 375 380
Asn Val Thr Leu Thr Gly Asp Phe Glu Pro Tyr Glu Lys Gly Ala Leu
385 390 395 400
Trp Gln Arg Ile Ala Asp Gln Gly Ala Leu Ile Gly Lys Asn Val Thr
405 410 415
Lys Lys Thr Thr Ile Leu Val Ala Gly Pro Trp Ala Thr Ile Thr Ser
420 425 430
Lys Gln Lys Arg Ala Glu Glu Leu Lys Glu Lys Gly Gln Asp Ile Gln
435 440 445
Ile Trp Asp Glu Lys Gln Leu Phe Thr Ala Leu Gly Leu Asp Glu Gln
450 455 460
Pro Pro Phe
465
<210>23
<211>496
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(466)
<223>RXA01280
<400>23
actttttcaa atccttcgcc atcgacaagc tcagccttcg tgttcgtccc ccgggcgtca 60
cgtcagcagt taaagaacaa ctccgaaata aggatggttc atg cca act att cag 115
Met Pro Thr Ile Gln
1 5
cag ctg gtc cgt aag gcc cgc cac gat aag tcc gac aag gtg gct acc 163
Gln Leu Val Arg Lys Ala Arg His Asp Lys Ser Asp Lys Val Ala Thr
10 15 20
gcg gca ctg aag ggt tcc cct cag cgt cgt ggc gta tgc acc cgt gtg 211
Ala Ala Leu Lys Gly Ser Pro Gln Arg Arg Gly Val Cys Thr Arg Val
25 30 35
tac acc acc acc cct aag aag cct aac tct gct ctt cgt aag gtc gct 259
Tyr Thr Thr Thr Pro Lys Lys Pro Asn Ser Ala Leu Arg Lys Val Ala
40 45 50
cgt gtg cgc ctt acc tcc ggc atc gag gtt tcc gct tac atc cct ggt 307
Arg Val Arg Leu Thr Ser Gly Ile Glu Val Ser Ala Tyr Ile Pro Gly
55 60 65
gag ggc cac aac ctg cag gag cac tcc atg gtg ctc gtt cgc ggt ggt 355
Glu Gly His Asn Leu Gln Glu His Ser Met Val Leu Val Arg Gly Gly
70 75 80 85
cgt gtt aag gac ctc cca ggt gtc cgt tac aag atc gtc cgt ggc gca 403
Arg Val Lys Asp Leu Pro Gly Val Arg Tyr Lys Ile Val Arg Gly Ala
90 95 100
ctg gat acc cag ggt gtt aag gac cgc aag cag gct cgt tcc cgc tac 451
Leu Asp Thr Gln Gly Val Lys Asp Arg Lys Gln Ala Arg Ser Arg Tyr
105 110 115
ggc gcg aag agg gga taattaaaaa tgcgtaaatc agcagctcct 496
Gly Ala Lys Arg Gly
120
<210>24
<211>122
<212>PRT
<213>谷氨酸棒状杆菌
<400>24
Met Pro Thr Ile Gln Gln Leu Val Arg Lys Ala Arg His Asp Lys Ser
1 5 10 15
Asp Lys Val Ala Thr Ala Ala Leu Lys Gly Ser Pro Gln Arg Arg Gly
20 25 30
Val Cys Thr Arg Val Tyr Thr Thr Thr Pro Lys Lys Pro Asn Ser Ala
35 40 45
Leu Arg Lys Val Ala Arg Val Arg Leu Thr Ser Gly Ile Glu Val Ser
50 55 60
Ala Tyr Ile Pro Gly Glu Gly His Asn Leu Gln Glu His Ser Met Val
65 70 75 80
Leu Val Arg Gly Gly Arg Val Lys Asp Leu Pro Gly Val Arg Tyr Lys
85 90 95
Ile Val Arg Gly Ala Leu Asp Thr Gln Gly Val Lys Asp Arg Lys Gln
100 105 110
Ala Arg Ser Arg Tyr Gly Ala Lys Arg Gly
115 120
<210>25
<211>1996
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1966)
<223>RXA01305
<400>25
ttctcaaggc tccctctcct ttaagttcgt ggccccagcg ccagcttttt tcataaaaac 60
tcaacaggca ctgtgggacc ccttcacttt gaaagacatc atg cgc ccc tct tcc 115
Met Arg Pro Ser Ser
1 5
cgg cca ctt ggc ctc gtc cta tgc acc gca ctg gca tca acg atc atc 163
Arg Pro Leu Gly Leu Val Leu Cys Thr Ala Leu Ala Ser Thr Ile Ile
10 15 20
acc gtt ccc gca gcg tcc gcc cag gag cca gcg ctt ctc gat gcc tcc 211
Thr Val Pro Ala Ala Ser Ala Gln Glu Pro Ala Leu Leu Asp Ala Ser
25 30 35
gcc atc gcc cca cat acc gcc agc tac ggc tac tac gtt gat gca tgg 259
Ala Ile Ala Pro His Thr Ala Ser Tyr Gly Tyr Tyr Val Asp Ala Trp
40 45 50
gac acc aac gtt tcc act gat ctg aat cca tca agt aat gca gct gtt 307
Asp Thr Asn Val Ser Thr Asp Leu Asn Pro Ser Ser Asn Ala Ala Val
55 60 65
ggc gta ctg gag gaa atg ctt gag ctg tgg acc cca ggc gaa gaa tgg 355
Gly Val Leu Glu Glu Met Leu Glu Leu Trp Thr Pro Gly Glu Glu Trp
70 75 80 85
aac acc ggc gtc aag gtt gac ccc acc gtg ctg gat tcc aac atc gca 403
Asn Thr Gly Val Lys Val Asp Pro Thr Val Leu Asp Ser Asn Ile Ala
90 95 100
cag tct gtg gca atc tcc cag cag gcg acc gat gct cag caa gaa cgt 451
Gln Ser Val Ala Ile Ser Gln Gln Ala Thr Asp Ala Gln Gln Glu Arg
105 110 115
gct tgg gtt att gat cgc cgc aac cag aac tac acc gca acc gac ggt 499
Ala Trp Val Ile Asp Arg Arg Asn Gln Asn Tyr Thr Ala Thr Asp Gly
120 125 130
ctt ggc gca tac gca gat agt tac cgc gag acc gca cag gtg ggc acc 547
Leu Gly Ala Tyr Ala Asp Ser Tyr Arg Glu Thr Ala Gln Val Gly Thr
135 140 145
acc atc cct gac gtt gtt cca gct gat gcc acc acc gtg aag tac aac 595
Thr Ile Pro Asp Val Val Pro Ala Asp Ala Thr Thr Val Lys Tyr Asn
150 155 160 165
gat ggc ggc aat gtg aat ggc aat tgg gca gag acc ggt gga gaa ctc 643
Asp Gly Gly Asn Val Asn Gly Asn Trp Ala Glu Thr Gly Gly Glu Leu
170 175 180
gga tcc act gtt gat cta att gaa gct atc cgt cag cat gcc gca acc 691
Gly Ser Thr Val Asp Leu Ile Glu Ala Ile Arg Gln His Ala Ala Thr
185 190 195
agc aac aat gcc aag gcg tac tac caa tac cca cgc ccc tac cgc tgg 739
Ser Asn Asn Ala Lys Ala Tyr Tyr Gln Tyr Pro Arg Pro Tyr Arg Trp
200 205 210
act gaa tcc atc gaa cca gaa gcc tgg ggc gag ggc gtt gac atg cca 787
Thr Glu Ser Ile Glu Pro Glu Ala Trp Gly Glu Gly Val Asp Met Pro
215 220 225
gag tat gca aac cca ctg cgc aag gat gaa tcc gaa gct gcc agc gat 835
Glu Tyr Ala Asn Pro Leu Arg Lys Asp Glu Ser Glu Ala Ala Ser Asp
230 235 240 245
ggc ggt ttc cct tcc gga cac acc tcc gca ggc ggc atg gca acc aac 883
Gly Gly Phe Pro Ser Gly His Thr Ser Ala Gly Gly Met Ala Thr Asn
250 255 260
ggc ctg gct tac gct ttc cca cag caa tac gat aaa ctg ctc atg act 931
Gly Leu Ala Tyr Ala Phe Pro Gln Gln Tyr Asp Lys Leu Leu Met Thr
265 270 275
gca gcg gaa atc ggc gaa agc cgc atc cag ctg ggc atg cac tct ccg 979
Ala Ala Glu Ile Gly Glu Ser Arg Ile Gln Leu Gly Met His Ser Pro
280 285 290
ctt gat gtt att ggc ggc cgt gtt cta tcc acc gcg att act gca ggt 1027
Leu Asp Val Ile Gly Gly Arg Val Leu Ser Thr Ala Ile Thr Ala Gly
295 300 305
gca ctt aat gat ccg aat ctc gac tcg gtg aag gct gaa gcc ttc gat 1075
Ala Leu Asn Asp Pro Asn Leu Asp Ser Val Lys Ala Glu Ala Phe Asp
310 315 320 325
gat gct cag gca tgg atc agt aac cag agc gac atc acc acc aac act 1123
Asp Ala Gln Ala Trp Ile Ser Asn Gln Ser Asp Ile Thr Thr Asn Thr
330 335 340
cgc gat ttt gat gag caa ctc gcc gag tac acc aac ttc ctc acc ttc 1171
Arg Asp Phe Asp Glu Gln Leu Ala Glu Tyr Thr Asn Phe Leu Thr Phe
345 350 355
ggc ttc gag cag tcc ggc gac acc acc caa gac atg cgc gtg cca aag 1219
Gly Phe Glu Gln Ser Gly Asp Thr Thr Gln Asp Met Arg Val Pro Lys
360 365 370
gga gct gag gct ctg ctg gaa acc cgc ctt ccg tac ctt gat gac gaa 1267
Gly Ala Glu Ala Leu Leu Glu Thr Arg Leu Pro Tyr Leu Asp Asp Glu
375 380 385
cag cgc cgt tgg gtt cta cat tcc act ggc ctc gag tcc ggt ttc cca 1315
Gln Arg Arg Trp Val Lau His Ser Thr Gly Leu Glu Ser Gly Phe Pro
390 395 400 405
gta ctt gat gat gcc gaa ggt tgg ggc cgt ctc aac ctc tac gct gcc 1363
Val Leu Asp Asp Ala Glu Gly Trp Gly Arg Leu Asn Leu Tyr Ala Ala
410 415 420
cag gct ggc tac agt gca ttc gat acc aac gtt gac gtc acc atg aat 1411
Gln Ala Gly Tyr Ser Ala Phe Asp Thr Asn Val Asp Val Thr Met Asn
425 430 435
gcc atc gac ggt ggc tac aac gcc aaa gac aac tgg caa aac gac atc 1459
Ala Ile Asp Gly Gly Tyr Asn Ala Lys Asp Asn Trp Gln Asn Asp Ile
440 445 450
gag ggc gca gga tcc ctg acc aag aac ggt tcc ggt gaa ctc acc ctg 1507
Glu Gly Ala Gly Ser Leu Thr Lys Asn Gly Ser Gly Glu Leu Thr Leu
455 460 465
tca ggt gac aac tcc tac acc ggt gga acc acc atc acc gcg ggc acc 1555
Ser Gly Asp Asn Ser Tyr Thr Gly Gly Thr Thr Ile Thr Ala Gly Thr
470 475 480 485
ttg gtt gct gca act gag tca gct ctg gga gca ggc gat ctc acc atc 1603
Leu Val Ala Ala Thr Glu Ser Ala Leu Gly Ala Gly Asp Leu Thr Ile
490 495 500
aac gat ggt gca acc ttg aag atc acc cag cct gtc acc gtg gat gga 1651
Asn Asp Gly Ala Thr Leu Lys Ile Thr Gln Pro Val Thr Val Asp Gly
505 510 515
acc gca aac ctg gga gga act ctg cac gtt gcc ctt cct gtt ggc acc 1699
Thr Ala Asn Leu Gly Gly Thr Leu His Val Ala Leu Pro Val Gly Thr
520 525 530
aac cac gtc acc gtg atc gat gct gca tca att tcc ggt gaa ttt gat 1747
Asn His Val Thr Val Ile Asp Ala Ala Ser Ile Ser Gly Glu Phe Asp
535 540 545
gag gtt att gtt gat ggc gca gtt gac gct cag gtg agc tac gac aac 1795
Glu Val Ile Val Asp Gly Ala Val Asp Ala Gln Val Ser Tyr Asp Asn
550 555 560 565
ggc tct gtc gtg att act aca ggc gca cct tct gat gac gta aag gaa 1843
Gly Ser Val Val Ile Thr Thr Gly Ala Pro Ser Asp Asp Val Lys Glu
570 575 580
act ggc tct tct gct ggc gga att ctt gcc atc gtg gca gcc ctg ggt 1891
Thr Gly Ser Ser Ala Gly Gly Ile Leu Ala Ile Val Ala Ala Leu Gly
585 590 595
ggc att gca gca ctg atc ttc ggt gca ttc acc cag ttt ggt ttc cca 1939
Gly Ile Ala Ala Leu Ile Phe Gly Ala Phe Thr Gln Phe Gly Phe Pro
600 605 610
cca gca atc aag gaa atg ttc gac ctt taagccttcg ccaaccccac 1986
Pro Ala Ile Lys Glu Met Phe Asp Leu
615 620
ggcgtgcttt 1996
<210>26
<211>622
<212>PRT
<213>谷氨酸棒状杆菌
<400>26
Met Arg Pro Ser Ser Arg Pro Leu Gly Leu Val Leu Cys Thr Ala Leu
1 5 10 15
Ala Ser Thr Ile Ile Thr Val Pro Ala Ala Ser Ala Gln Glu Pro Ala
20 25 30
Leu Leu Asp Ala Ser Ala Ile Ala Pro His Thr Ala Ser Tyr Gly Tyr
35 40 45
Tyr Val Asp Ala Trp Asp Thr Asn Val Ser Thr Asp Leu Asn Pro Ser
50 55 60
Ser Asn Ala Ala Val Gly Val Leu Glu Glu Met Leu Glu Leu Trp Thr
65 70 75 80
Pro Gly Glu Glu Trp Asn Thr Gly Val Lys Val Asp Pro Thr Val Leu
85 90 95
Asp Ser Asn Ile Ala Gln Ser Val Ala Ile Ser Gln Gln Ala Thr Asp
100 105 110
Ala Gln Gln Glu Arg Ala Trp Val Ile Asp Arg Arg Asn Gln Asn Tyr
115 120 125
Thr Ala Thr Asp Gly Leu Gly Ala Tyr Ala Asp Ser Tyr Arg Glu Thr
130 135 140
Ala Gln Val Gly Thr Thr Ile Pro Asp Val Val Pro Ala Asp Ala Thr
145 150 155 160
Thr Val Lys Tyr Asn Asp Gly Gly Asn Val Asn Gly Asn Trp Ala Glu
165 170 175
Thr Gly Gly Glu Leu Gly Ser Thr Val Asp Leu Ile Glu Ala Ile Arg
180 185 190
Gln His Ala Ala Thr Ser Asn Asn Ala Lys Ala Tyr Tyr Gln Tyr Pro
195 200 205
Arg Pro Tyr Arg Trp Thr Glu Ser Ile Glu Pro Glu Ala Trp Gly Glu
210 215 220
Gly Val Asp Met Pro Glu Tyr Ala Asn Pro Leu Arg Lys Asp Glu Ser
225 230 235 240
Glu Ala Ala Ser Asp Gly Gly Phe Pro Ser Gly His Thr Ser Ala Gly
245 250 255
Gly Met Ala Thr Asn Gly Leu Ala Tyr Ala Phe Pro Gln Gln Tyr Asp
260 265 270
Lys Leu Leu Met Thr Ala Ala Glu Ile Gly Glu Ser Arg Ile Gln Leu
275 280 285
Gly Met His Ser Pro Leu Asp Val Ile Gly Gly Arg Val Leu Ser Thr
290 295 300
Ala Ile Thr Ala Gly Ala Leu Asn Asp Pro Asn Leu Asp Ser Val Lys
305 310 315 320
Ala Glu Ala Phe Asp Asp Ala Gln Ala Trp Ile Ser Asn Gln Ser Asp
325 330 335
Ile Thr Thr Asn Thr Arg Asp Phe Asp Glu Gln Leu Ala Glu Tyr Thr
340 345 350
Asn Phe Leu Thr Phe Gly Phe Glu Gln Ser Gly Asp Thr Thr Gln Asp
355 360 365
Met Arg Val Pro Lys Gly Ala Glu Ala Leu Leu Glu Thr Arg Leu Pro
370 375 380
Tyr Leu Asp Asp Glu Gln Arg Arg Trp Val Leu His Ser Thr Gly Leu
385 390 395 400
Glu Ser Gly Phe Pro Val Leu Asp Asp Ala Glu Gly Trp Gly Arg Leu
405 410 415
Asn Leu Tyr Ala Ala Gln Ala Gly Tyr Ser Ala Phe Asp Thr Asn Val
420 425 430
Asp Val Thr Met Asn Ala Ile Asp Gly Gly Tyr Asn Ala Lys Asp Asn
435 440 445
Trp Gln Asn Asp Ile Glu Gly Ala Gly Ser Leu Thr Lys Asn Gly Ser
450 455 460
Gly Glu Leu Thr Leu Ser Gly Asp Asn Ser Tyr Thr Gly Gly Thr Thr
465 470 475 480
Ile Thr Ala Gly Thr Leu Val Ala Ala Thr Glu Ser Ala Leu Gly Ala
485 490 495
Gly Asp Leu Thr Ile Asn Asp Gly Ala Thr Leu Lys Ile Thr Gln Pro
500 505 510
Val Thr Val Asp Gly Thr Ala Asn Leu Gly Gly Thr Leu His Val Ala
515 520 525
Leu Pro Val Gly Thr Asn His Val Thr Val Ile Asp Ala Ala Ser Ile
530 535 540
Ser Gly Glu Phe Asp Glu Val Ile Val Asp Gly Ala Val Asp Ala Gln
545 550 555 560
Val Ser Tyr Asp Asn Gly Ser Val Val Ile Thr Thr Gly Ala Pro Ser
565 570 575
Asp Asp Val Lys Glu Thr Gly Ser Ser Ala Gly Gly Ile Leu Ala Ile
580 585 590
Val Ala Ala Leu Gly Gly Ile Ala Ala Leu Ile Phe Gly Ala Phe Thr
595 600 605
Gln Phe Gly Phe Pro Pro Ala Ile Lys Glu Met Phe Asp Leu
610 615 620
<210>27
<211>838
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(808)
<223>RXA01343
<400>27
ttcaaaatga atggccacca accaattttc accaaagttt tatgtggcag ggccagctcc 60
ggcccgttaa accacagaat tccatgaaag ggaatttcta atg agc aag aac tct 115
Met Ser Lys Asn Ser
1 5
aag gcg tac cgc gag gcc gct gag aag atc gac gct ggt cgc atc tac 163
Lys Ala Tyr Arg Glu Ala Ala Glu Lys Ile Asp Ala Gly Arg Ile Tyr
10 15 20
tcc cca ctc gag gct gca aac ctg gtc aag gag acc tcc tcc aag aac 211
Ser Pro Leu Glu Ala Ala Asn Leu Val Lys Glu Thr Ser Ser Lys Asn
25 30 35
tac gac gct tcc atc gac gta gct atc cgc ctg ggc gtt gac cca cgt 259
Tyr Asp Ala Ser Ile Asp Val Ala Ile Arg Leu Gly Val Asp Pro Arg
40 45 50
aag gct gat cag ctt gtt cgt ggc acc gtc tcc ctg cct aac ggc acc 307
Lys Ala Asp Gln Leu Val Arg Gly Thr Val Ser Leu Pro Asn Gly Thr
55 60 65
ggt aag acc gtt cgc gtt gct gtg ttc gca cag ggc gag aag gct act 355
Gly Lys Thr Val Arg Val Ala Val Phe Ala Gln Gly Glu Lys Ala Thr
70 75 80 85
gag gct gag gct gct ggc gct gac ttc gtt ggc acc gac gag ctc gtt 403
Glu Ala Glu Ala Ala Gly Ala Asp Phe Val Gly Thr Asp Glu Leu Val
90 95 100
gag aag atc cag ggt ggc tgg acc gac ttc gac gtt gct att gca acc 451
Glu Lys Ile Gln Gly Gly Trp Thr Asp Phe Asp Val Ala Ile Ala Thr
105 110 115
cct gat cag atg gct aag atc ggc cgt atc gct cgt gtc ttg ggc cca 499
Pro Asp Gln Met Ala Lys Ile Gly Arg Ile Ala Arg Val Leu Gly Pro
120 125 130
cgt ggt ctg atg cct aac cct aag acc ggc acc gtc acc aac gat gtc 547
Arg Gly Leu Met Pro Asn Pro Lys Thr Gly Thr Val Thr Asn Asp Val
135 140 145
gct aag gct atc gaa gag gtc aag ggc ggc aag att tcc ttc cgc gtt 595
Ala Lys Ala Ile Glu Glu Val Lys Gly Gly Lys Ile Ser Phe Arg Val
150 155 160 165
gac aag gct tcc aac ctg cac gct gca att ggt aag gct tcc ttc gat 643
Asp Lys Ala Ser Asn Leu His Ala Ala Ile Gly Lys Ala Ser Phe Asp
170 175 180
gcg aag aag ctg gct gag aac tac ggc gct ctc ctc gac gag atc atc 691
Ala Lys Lys Leu Ala Glu Asn Tyr Gly Ala Leu Leu Asp Glu Ile Ile
185 190 195
cgt atc aag cct tct tcc gct aag ggc atc tac gtc aag cgc gtg acc 739
Arg Ile Lys Pro Ser Ser Ala Lys Gly Ile Tyr Val Lys Arg Val Thr
200 205 210
ctg tct tcc acc acc ggt cct ggt gtt gag gtt gac act cac gtc acc 787
Leu Ser Ser Thr Thr Gly Pro Gly Val Glu Val Asp Thr His Val Thr
215 220 225
aag aac tac gca gaa gag gca taagccttcc cacgcgtaac tctttaggcc 838
Lys Asn Tyr Ala Glu Glu Ala
230 235
<210>28
<211>236
<212>PRT
<213>谷氨酸棒状杆菌
<400>28
Met Ser Lys Asn Ser Lys Ala Tyr Arg Glu Ala Ala Glu Lys Ile Asp
1 5 10 15
Ala Gly Arg Ile Tyr Ser Pro Leu Glu Ala Ala Asn Leu Val Lys Glu
20 25 30
Thr Ser Ser Lys Asn Tyr Asp Ala Ser Ile Asp Val Ala Ile Arg Leu
35 40 45
Gly Val Asp Pro Arg Lys Ala Asp Gln Leu Val Arg Gly Thr Val Ser
50 55 60
Leu Pro Asn Gly Thr Gly Lys Thr Val Arg Val Ala Val Phe Ala Gln
65 70 75 80
Gly Glu Lys Ala Thr Glu Ala Glu Ala Ala Gly Ala Asp Phe Val Gly
85 90 95
Thr Asp Glu Leu Val Glu Lys Ile Gln Gly Gly Trp Thr Asp Phe Asp
100 105 110
Val Ala Ile Ala Thr Pro Asp Gln Met Ala Lys Ile Gly Arg Ile Ala
115 120 125
Arg Val Leu Gly Pro Arg Gly Leu Met Pro Asn Pro Lys Thr Gly Thr
130 135 140
Val Thr Asn Asp Val Ala Lys Ala Ile Glu Glu Val Lys Gly Gly Lys
145 150 155 160
Ile Ser Phe Arg Val Asp Lys Ala Ser Asn Leu His Ala Ala Ile Gly
165 170 175
Lys Ala Ser Phe Asp Ala Lys Lys Leu Ala Glu Asn Tyr Gly Ala Leu
180 185 190
Leu Asp Glu Ile Ile Arg Ile Lys Pro Ser Ser Ala Lys Gly Ile Tyr
195 200 205
Val Lys Arg Val Thr Leu Ser Ser Thr Thr Gly Pro Gly Val Glu Val
210 215 220
Asp Thr His Val Thr Lys Asn Tyr Ala Glu Glu Ala
225 230 235
<210>29
<211>2074
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2044)
<223>RXA01480
<400>29
ggctggtggc ttcacctgaa gtggacagat caattccaaa ccatgcagca gcgaggacaa 60
cgatgactcc gagcactccg ccgagggtct tcttgctgtt ttg cat aca acc aat 115
Leu His Thr Thr Asn
1 5
ata ggc aca cta aag gga cgg gct aaa gtg atg gtc atg gca aag gga 163
Ile Gly Thr Leu Lys Gly Arg Ala Lys Val Met Val Met Ala Lys Gly
10 15 20
cgt att ccg gaa agt gac att cag gca atc cgc gag cgc acc ccg atc 211
Arg Ile Pro Glu Ser Asp Ile Gln Ala Ile Arg Glu Arg Thr Pro Ile
25 30 35
gag gag atc gtg ggt gat tatgtg cag ctg aaa tcg gca ggt gct gat 259
Glu GluIle Val Gly Asp Tyr Val Gln Leu Lys Ser Ala Gly Ala Asp
40 45 50
tcg ctc aag ggg ctt tct ccc ttt aaa gat gaa aaa aca cca tcg ttc 307
Ser Leu Lys Gly Leu Ser Pro Phe Lys Asp Glu Lys Thr Pro Ser Phe
55 60 65
cat gtg cgc ccc aac cgt ggt tac tac cac tgt ttc tcc acc ggc aaa 355
His Val Arg Pro Asn Arg Gly Tyr Tyr His Cys Phe Ser Thr Gly Lys
70 75 80 85
ggt gga gat gtg ttc tcc ttc ctc atg gag atg gaa cac atc tct ttc 403
Gly Gly Asp Val Phe Ser Phe Leu Met Glu Met Glu His Ile Ser Phe
90 95 100
cct gag gct gtg gaa gtg tgt gcg gag aaa att ggt tat cag atc aat 451
Pro Glu Ala Val Glu Val Cys Ala Glu Lys Ile Gly Tyr Gln Ile Asn
105 110 115
tac caa ggt ggt ggc ccg ggg cgc cgt gag gaa cct gga act cgc cag 499
Tyr Gln Gly Gly Gly Pro Gly Arg Arg Glu Glu Pro Gly Thr Arg Gln
120 125 130
cgc ctt att ttg gct aat aag gct gcg cac cag ttt tat cgc gag caa 547
Arg Leu Ile Leu Ala Asn Lys Ala Ala His Gln Phe Tyr Arg Glu Gln
135 140 145
tta gaa acc ccc gaa gca caa cct ggc agg gag ttt ttg ctg cag cgt 595
Leu Glu Thr Pro Glu Ala Gln Pro Gly Arg Glu Phe Leu Leu Gln Arg
150 155 160 165
gga ttc gga cag cag cac att tat cat ttc gaa tgt ggc tat gcg cct 643
Gly Phe Gly Gln Gln His Ile Tyr His Phe Glu Cys Gly Tyr Ala Pro
170 175 180
gcc ggc tgg gat acc ttg acc aag cat ttg ctg aag aag ggc ttt gag 691
Ala Gly Trp Asp Thr Leu Thr Lys His Leu Leu Lys Lys Gly Phe Glu
185 190 195
ttc aag gaa tta gaa gct gcc ggt cta agc aag atg ggt aag cgc ggt 739
Phe Lys Glu Leu Glu Ala Ala Gly Leu Ser Lys Met Gly Lys Arg Gly
200 205 210
ccg att gat cag ttc cag cgc agg ttg ctg tgg ccg atc aag aac ctg 787
Pro Ile Asp Gln Phe Gln Arg Arg Leu Leu Trp Pro Ile Lys Asn Leu
215 220 225
tct ggt gat gtc att ggt ttt ggc gcc cgc aag ctt ttc gat gac gac 835
Ser Gly Asp Val Ile Gly Phe Gly Ala Arg Lys Leu Phe Asp Asp Asp
230 235 240 245
aaa atg ggc aag tac atg aat acg cct gag acg ttg ttg tac aaa aag 883
Lys Met Gly Lys Tyr Met Asn Thr Pro Glu Thr Leu Leu Tyr Lys Lys
250 255 260
tcc aag gtg ctc ttt ggt cta gat tct gca aag aag gcc att gca gct 931
Ser Lys Val Leu Phe Gly Leu Asp Ser Ala Lys Lys Ala Ile Ala Ala
265 270 275
ggc cac caa gca gta gtg gtg gaa ggc tac acc gat gtg atg gcc atg 979
Gly His Gln Ala Val Val Val Glu Gly Tyr Thr Asp Val Met Ala Met
280 285 290
cat gcc gcg ggc att gat aca gcc gtg gca tcg tgt ggc act gcg ttt 1027
His Ala Ala Gly Ile Asp Thr Ala Val Ala Ser Cys Gly Thr Ala Phe
295 300 305
ggt gaa gaa cac ttg cag atg ctt cgt cga ctc atg ctg gat gat aac 1075
Gly Glu Glu His Leu Gln Met Leu Arg Arg Leu Met Leu Asp Asp Asn
310 315 320 325
tac ttc cgc ggt gaa ctg att tac acc ttc gat ggt gat gag gcc ggc 1123
Tyr Phe Arg Gly Glu Leu Ile Tyr Thr Phe Asp Gly Asp Glu Ala Gly
330 335 340
cag aag gcc gcc atg cgt gcc ttt gag ggc gat cag aag ttc aca gga 1171
Gln Lys Ala Ala Met Arg Ala Phe Glu Gly Asp Gln Lys Phe Thr Gly
345 350 355
caa tca ttt gtg tct gtg gca ccc aac ggc atg gat ccg tgt gat ctg 1219
Gln Ser Phe Val Ser Val Ala Pro Asn Gly Met Asp Pro Cys Asp Leu
360 365 370
cgc ctt gag cgt ggc gat gcg gcg gtg cgt gat ctt gtg gca cga cgc 1267
Arg Leu Glu Arg Gly Asp Ala Ala Val Arg Asp Leu Val Ala Arg Arg
375 380 385
atc ccg atg ttt gag ttc gtc atc caa tcg atc atc agc gaa tac acc 1315
Ile Pro Met Phe Glu Phe Val Ile Gln Ser Ile Ile Ser Glu Tyr Thr
390 395 400 405
ctc gac acc gtg gaa ggc cgt ctg gct gcg ctt cgt cgg gca gtc ccc 1363
Leu Asp Thr Val Glu Gly Arg Leu Ala Ala Leu Arg Arg Ala Val Pro
410 415 420
atc gtg gcg gat att cgc gat aag acg ctg cag tct gaa tac gcc cgc 1411
Ile Val Ala Asp Ile Arg Asp Lys Thr Leu Gln Ser Glu Tyr Ala Arg
425 430 435
ctg ctg tct ggt tgg gtc ggc tgg tct gat cct tca gag gtg ctg cgt 1459
Leu Leu Ser Gly Trp Val Gly Trp Ser Asp Pro Ser Glu Val Leu Arg
440 445 450
cag gtt cac gag gaa gca cgt cgc ccc aag cgc gat aag aag cct gtg 1507
Gln Val His Glu Glu Ala Arg Arg Pro Lys Arg Asp Lys Lys Pro Val
455 460 465
cgt gca aag cgt ttc gat caa ccg ctc gag gat caa agc ctg cga ccc 1555
Arg Ala Lys Arg Phe Asp Gln Pro Leu Glu Asp Gln Ser Leu Arg Pro
470 475 480 485
acc atg gcg ctg cct aat ccg cgg aac cct gtg ctg tgg cag gaa cgg 1603
Thr Met Ala Leu Pro Asn Pro Arg Asn Pro Val Leu Trp Gln Glu Arg
490 495 500
gaa tca ctc aag atc gcc ctg caa tat ccg gag ctc gcg gga tcg tac 1651
Glu Ser Leu Lys Ile Ala Leu Gln Tyr Pro Glu Leu Ala Gly Ser Tyr
505 510 515
ttt gat gga ctg cca acc gat agc ttc acc aac cct gcc tac cgc atg 1699
Phe Asp Gly Leu Pro Thr Asp Ser Phe Thr Asn Pro Ala Tyr Arg Met
520 525 530
gta cgc gat gcc att tcg gct gct ggg gga tgt gaa cgt gcc ctc gat 1747
Val Arg Asp Ala Ile Ser Ala Ala Gly Gly Cys Glu Arg Ala Leu Asp
535 540 545
ggc act gat tgg ttg cct gcc gta tcg gaa aat atg act gat att ttg 1795
Gly Thr Asp Trp Leu Pro Ala Val Ser Glu Asn Met Thr Asp Ile Leu
550 555 560 565
ggc acg tcg ttg gtg tca gag ctg gcg atg gaa ccc atc gag gtg gaa 1843
Gly Thr Ser Leu Val Ser Glu Leu Ala Met Glu Pro Ile Glu Val Glu
570 575 580
gcg caa gac ctg gaa tca tat acc gat ggt gtg ttg tcc agg ctg cag 1891
Ala Gln Asp Leu Glu Ser Tyr Thr Asp Gly Val Leu Ser Arg Leu Gln
585 590 595
gaa aca cga gtg ggc aac cag atc gcc atc ttg aaa gga cag ctg caa 1939
Glu Thr Arg Val Gly Asn Gln Ile Ala Ile Leu Lys Gly Gln Leu Gln
600 605 610
aga atg cgt ccg tct gaa gat gag caa gcc tac aac tcg ctg ttt tcc 1987
Arg Met Arg Pro Ser Glu Asp Glu Gln Ala Tyr Asn Ser Leu Phe Ser
615 620 625
gat ctg gtt gcc ctg gaa cag gcg cgc cgg gag ctg ttg gcc cgg gcg 2035
Asp Leu Val Ala Leu Glu Gln Ala Arg Arg Glu Leu Leu Ala Arg Ala
630 635 640 645
ttt aga ggg taatttagtc ctggtcttgc tcgaggactt 2074
Phe Arg Gly
<210>30
<211>648
<212>PRT
<213>谷氨酸棒状杆菌
<400>30
Leu His Thr Thr Asn Ile Gly Thr Leu Lys Gly Arg Ala Lys Val Met
1 5 10 15
Val Met Ala Lys Gly Arg Ile Pro Glu Ser Asp Ile Gln Ala Ile Arg
20 25 30
Glu Arg Thr Pro Ile Glu Glu Ile Val Gly Asp Tyr Val Gln Leu Lys
35 40 45
Ser Ala Gly Ala Asp Ser Leu Lys Gly Leu Ser Pro Phe Lys Asp Glu
50 55 60
Lys Thr Pro Ser Phe His Val Arg Pro Asn Arg Gly Tyr Tyr His Cys
65 70 75 80
Phe Ser Thr Gly Lys Gly Gly Asp Val Phe Ser Phe Leu Met Glu Met
85 90 95
Glu His Ile Ser Phe Pro Glu Ala Val Glu Val Cys Ala Glu Lys Ile
100 105 110
Gly Tyr Gln Ile Asn Tyr Gln Gly Gly Gly Pro Gly Arg Arg Glu Glu
115 120 125
Pro Gly Thr Arg Gln Arg Leu Ile Leu Ala Asn Lys Ala Ala His Gln
130 135 140
Phe Tyr Arg Glu Gln Leu Glu Thr Pro Glu Ala Gln Pro Gly Arg Glu
145 150 155 160
Phe Leu Leu Gln Arg Gly Phe Gly Gln Gln His Ile Tyr His Phe Glu
165 170 175
Cys Gly Tyr Ala Pro Ala Gly Trp Asp Thr Leu Thr Lys His Leu Leu
180 185 190
Lys Lys Gly Phe Glu Phe Lys Glu Leu Glu Ala Ala Gly Leu Ser Lys
195 200 205
Met Gly Lys Arg Gly Pro Ile Asp Gln Phe Gln Arg Arg Leu Leu Trp
210 215 220
Pro Ile Lys Asn Leu Ser Gly Asp Val Ile Gly Phe Gly Ala Arg Lys
225 230 235 240
Leu Phe Asp Asp Asp Lys Met Gly Lys Tyr Met Asn Thr Pro Glu Thr
245 250 255
Leu Leu Tyr Lys Lys Ser Lys Val Leu Phe Gly Leu Asp Ser Ala Lys
260 265 270
Lys Ala Ile Ala Ala Gly His Gln Ala Val Val Val Glu Gly Tyr Thr
275 280 285
Asp Val Met Ala Met His Ala Ala Gly Ile Asp Thr Ala Val Ala Ser
290 295 300
Cys Gly Thr Ala Phe Gly Glu Glu His Leu Gln Met Leu Arg Arg Leu
305 310 315 320
Met Leu Asp Asp Asn Tyr Phe Arg Gly Glu Leu Ile Tyr Thr Phe Asp
325 330 335
Gly Asp Glu Ala Gly Gln Lys Ala Ala Met Arg Ala Phe Glu Gly Asp
340 345 350
Gln Lys Phe Thr Gly Gln Ser Phe Val Ser Val Ala Pro Asn Gly Met
355 360 365
Asp Pro Cys Asp Leu Arg Leu Glu Arg Gly Asp Ala Ala Val Arg Asp
370 375 380
Leu Val Ala Arg Arg Ile Pro Met Phe Glu Phe Val Ile Gln Ser Ile
385 390 395 400
Ile Ser Glu Tyr Thr Leu Asp Thr Val Glu Gly Arg Leu Ala Ala Leu
405 410 415
Arg Arg Ala Val Pro Ile Val Ala Asp Ile Arg Asp Lys Thr Leu Gln
420 425 430
Ser Glu Tyr Ala Arg Leu Leu Ser Gly Trp Val Gly Trp Ser Asp Pro
435 440 445
Ser Glu Val Leu Arg Gln Val His Glu Glu Ala Arg Arg Pro Lys Arg
450 455 460
Asp Lys Lys Pro Val Arg Ala Lys Arg Phe Asp Gln Pro Leu Glu Asp
465 470 475 480
Gln Ser Leu Arg Pro Thr Met Ala Leu Pro Asn Pro Arg Asn Pro Val
485 490 495
Leu Trp Gln Glu Arg Glu Ser Leu Lys Ile Ala Leu Gln Tyr Pro Glu
500 505 510
Leu Ala Gly Ser Tyr Phe Asp Gly Leu Pro Thr Asp Ser Phe Thr Asn
515 520 525
Pro Ala Tyr Arg Met Val Arg Asp Ala Ile Ser Ala Ala Gly Gly Cys
530 535 540
Glu Arg Ala Leu Asp Gly Thr Asp Trp Leu Pro Ala Val Ser Glu Asn
545 550 555 560
Met Thr Asp Ile Leu Gly Thr Ser Leu Val Ser Glu Leu Ala Met Glu
565 570 575
Pro Ile Glu Val Glu Ala Gln Asp Leu Glu Ser Tyr Thr Asp Gly Val
580 585 590
Leu Ser Arg Leu Gln Glu Thr Arg Val Gly Asn Gln Ile Ala Ile Leu
595 600 605
Lys Gly Gln Leu Gln Arg Met Arg Pro Ser Glu Asp Glu Gln Ala Tyr
610 615 620
Asn Ser Leu Phe Ser Asp Leu Val Ala Leu Glu Gln Ala Arg Arg Glu
625 630 635 640
Leu Leu Ala Arg Ala Phe Arg Gly
645
<210>31
<211>1393
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1363)
<223>RXA01563
<400>31
ccgccaagtg cctaccaagt ggaaatgcgt gcgtcctgga ccgcgactgg caccgacctc 60
accgcgcatg ttgaagcgtt cgcagcagtg cttgcctctg ttg ctg gac ttc ccc 115
Leu Leu Asp Phe Pro
1 5
cag agg gcg tca ccg aac tac gaa ggt aga ttg gac acc atg gtt tcc 163
Gln Arg Ala Ser Pro Asn Tyr Glu Gly Arg Leu Asp Thr Met Val Ser
10 15 20
gat ctc ctt caa ccc cgc gac ggc att ccg cct ttg cta tct acc cct 211
Asp Leu Leu Gln Pro Arg Asp Gly Ile Pro Pro Leu Leu Ser Thr Pro
25 30 35
ggt gag ttc act gct gcg gca gat ctc ttg gct agc gga act ggg ccc 259
Gly Glu Phe Thr Ala Ala Ala Asp Leu Leu Ala Ser Gly Thr Gly Pro
40 45 50
ttc gcc att gat acg gaa cgc gcg tcc ggt ttt aga tac gat gac cgc 307
Phe Ala Ile Asp Thr Glu Arg Ala Ser Gly Phe Arg Tyr Asp Asp Arg
55 60 65
gca ttt ttg att cag atc cgg cgc cgt ggc agc gga act ctc cta ttc 355
Ala Phe Leu Ile Gln Ile Arg Arg Arg Gly Ser Gly Thr Leu Leu Phe
70 75 80 85
gac ccg gag cag ttc cgt cct gaa tta act cag gcg tta aag ccg gtg 403
Asp Pro Glu Gln Phe Arg Pro Glu Leu Thr Gln Ala Leu Lys Pro Val
90 95 100
ctc aat ggt caa gag tgg atc att cac gca gca agc acc gat ttg ccg 451
Leu Asn Gly Gln Glu Trp Ile Ile His Ala Ala Ser Thr Asp Leu Pro
105 110 115
agc ctt gcg tgg ctt gat ctt cac ccc gga tta ctc ttt gat aca gaa 499
Ser Leu Ala Trp Leu Asp Leu His Pro Gly Leu Leu Phe Asp Thr Glu
120 125 130
ctt gct ggc cgc tta gcc gga ttt gat cac gtt aat ctc gct gcc atg 547
Leu Ala Gly Arg Leu Ala Gly Phe Asp His Val Asn Leu Ala Ala Met
135 140 145
gtg gaa cag att ttt gat ctc cac ttg ctc aaa ggc cac ggt tcg gaa 595
Val Glu Gln Ile Phe Asp Leu His Leu Leu Lys Gly His Gly Ser Glu
150 155 160 165
gat tgg tcc aag cgt cct ctg ccg gaa tct tgg ctc aac tac gca gca 643
Asp Trp Ser Lys Arg Pro Leu Pro Glu Ser Trp Leu Asn Tyr Ala Ala
170 175 180
ctc gat gtg gag atg ctg ctg gag ctt gcc gat gtc atg gct gaa atc 691
Leu Asp Val Glu Met Leu Leu Glu Leu Ala Asp Val Met Ala Glu Ile
185 190 195
ctg gat cag cag gga aaa ctc ccc tgg gct gaa cag gaa ttt gtc cat 739
Leu Asp Gln Gln Gly Lys Leu Pro Trp Ala Glu Gln Glu Phe Val His
200 205 210
att gtg gat caa ttc gcc acg atg acc gaa cct tcc gaa acg tcc tgg 787
Ile Val Asp Gln Phe Ala Thr Met Thr Glu Pro Ser Glu Thr Ser Trp
215 220 225
cag gac ctt aaa ggg ctg tcc act ctc aaa cga cca gac caa tta gtt 835
Gln Asp Leu Lys Gly Leu Ser Thr Leu Lys Arg Pro Asp Gln Leu Val
230 235 240 245
gtg gcc cgt gaa atg tgg ttg gaa cgc gac tct ttc gca gcc tcc cgc 883
Val Ala Arg Glu Met Trp Leu Glu Arg Asp Ser Phe Ala Ala Ser Arg
250 255 260
gac ctg gcg ccc ggt aaa gtg ctg tcc aac aaa gtc atc gtg gaa gtc 931
Asp Leu Ala Pro Gly Lys Val Leu Ser Asn Lys Val Ile Val Glu Val
265 270 275
gcc cgt gtt ctc ccc cgc acc ccg gca gaa tta gcg cag gtc aag gga 979
Ala Arg Val Leu Pro Arg Thr Pro Ala Glu Leu Ala Gln Val Lys Gly
280 285 290
ttc ccc ggt cga tcc cag ggt gcc acc aaa cgc tgg ttc cgc atc atc 1027
Phe Pro Gly Arg Ser Gln Gly Ala Thr Lys Arg Trp Phe Arg Ile Ile
295 300 305
acc cgg gcg ctc aaa tcc cct cgc agg aac tgg cca aag cct cag cag 1075
Thr Arg Ala Leu Lys Ser Pro Arg Arg Asn Trp Pro Lys Pro Gln Gln
310 315 320 325
cgc aag gac ggc atc ccc gat cgt cgc gcg tgg gcg tcc tac tac cca 1123
Arg Lys Asp Gly Ile Pro Asp Arg Arg Ala Trp Ala Ser Tyr Tyr Pro
330 335 340
gaa gag cac gaa gtg ctc caa gag att aga gcg ctt atc gac gac ctc 1171
Glu Glu His Glu Val Leu Gln Glu Ile Arg Ala Leu Ile Asp Asp Leu
345 350 355
gcc gcc gat atc aac gtt ccc ggc gag aet atc ctt cag cct tca act 1219
Ala Ala Asp Ile Asn Val Pro Gly Glu Asn Ile Leu Gln Pro Ser Thr
360 365 370
ctg cga gta gct gtg tgg atg gct aaa cac acc ggc gag atc cat aat 1267
Leu Arg Val Ala Val Trp Met Ala Lys His Thr Gly Glu Ile His Asn
375 380 385
gct gaa aca ctc aac gct gta ctt cgc gat tat ggt gcc cgc cag tgg 1315
Ala Glu Thr Leu Asn Ala Val Leu Arg Asp Tyr Gly Ala Arg Gln Trp
390 395 400 405
caa att gac cag act ttt ccg att ctg tcc gcc aac ttg ctg aag ctc 1363
Gln Ile Asp Gln Thr Phe Pro Ile Leu Ser Ala Asn Leu Leu Lys Leu
410 415 420
taaacctaaa gcccgcggct aaggctgcgg 1393
<210>32
<211>421
<212>PRT
<213>谷氨酸棒状杆菌
<400>32
Leu Leu Asp Phe Pro Gln Arg Ala Ser Pro Asn Tyr Glu Gly Arg Leu
1 5 10 15
Asp Thr Met Val Ser Asp Leu Leu Gln Pro Arg Asp Gly Ile Pro Pro
20 25 30
Leu Leu Ser Thr Pro Gly Glu Phe Thr Ala Ala Ala Asp Leu Leu Ala
35 40 45
Ser Gly Thr Gly Pro Phe Ala Ile Asp Thr Glu Arg Ala Ser Gly Phe
50 55 60
Arg Tyr Asp Asp Arg Ala Phe Leu Ile Gln Ile Arg Arg Arg Gly Ser
65 70 75 80
Gly Thr Leu Leu Phe Asp Pro Glu Gln Phe Arg Pro Glu Leu Thr Gln
85 90 95
Ala Leu Lys Pro Val Leu Asn Gly Gln Glu Trp Ile Ile His Ala Ala
100 105 110
Ser Thr Asp Leu Pro Ser Leu Ala Trp Leu Asp Leu His Pro Gly Leu
115 120 125
Leu Phe Asp Thr Glu Leu Ala Gly Arg Leu Ala Gly Phe Asp His Val
130 135 140
Asn Leu Ala Ala Met Val Glu Gln Ile Phe Asp Leu His Leu Leu Lys
145 150 155 160
Gly His Gly Ser Glu Asp Trp Ser Lys Arg Pro Leu Pro Glu Ser Trp
165 170 175
Leu Asn Tyr Ala Ala Leu Asp Val Glu Met Leu Leu Glu Leu Ala Asp
180 185 190
Val Met Ala Glu Ile Leu Asp Gln Gln Gly Lys Leu Pro Trp Ala Glu
195 200 205
Gln Glu Phe Val His Ile Val Asp Gln Phe Ala Thr Met Thr Glu Pro
210 215 220
Ser Glu Thr Ser Trp Gln Asp Leu Lys Gly Leu Ser Thr Leu Lys Arg
225 230 235 240
Pro Asp Gln Leu Val Val Ala Arg Glu Met Trp Leu Glu Arg Asp Ser
245 250 255
Phe Ala Ala Ser Arg Asp Leu Ala Pro Gly Lys Val Leu Ser Asn Lys
260 265 270
Val Ile Val Glu Val Ala Arg Val Leu Pro Arg Thr Pro Ala Glu Leu
275 280 285
Ala Gln Val Lys Gly Phe Pro Gly Arg Ser Gln Gly Ala Thr Lys Arg
290 295 300
Trp Phe Arg Ile Ile Thr Arg Ala Leu Lys Ser Pro Arg Arg Asn Trp
305 310 315 320
Pro Lys Pro Gln Gln Arg Lys Asp Gly Ile Pro Asp Arg Arg Ala Trp
325 330 335
Ala Ser Tyr Tyr Pro Glu Glu His Glu Val Leu Gln Glu Ile Arg Ala
340 345 350
Leu Ile Asp Asp Leu Ala Ala Asp Ile Asn Val Pro Gly Glu Asn Ile
355 360 365
Leu Gln Pro Ser Thr Leu Arg Val Ala Val Trp Met Ala Lys His Thr
370 375 380
Gly Glu Ile His Asn Ala Glu Thr Leu Asn Ala Val Leu Arg Asp Tyr
385 390 395 400
Gly Ala Arg Gln Trp Gln Ile Asp Gln Thr Phe Pro Ile Leu Ser Ala
405 410 415
Asn Leu Leu Lys Leu
420
<210>33
<211>976
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(946)
<223>RXA01581
<400>33
tggtgttttc gaggtgcaga tcttccgcac cgcgatgggc aacaatgtgc atattcttat 60
acatcctttt ctgtgggctt tttaaggata gggagcaaag ttg acc gca cgg tta 115
Leu Thr Ala Arg Leu
1 5
act gtt agt gtt atg agc atg gca ttg gac ttt aat gag gcg ttt acc 153
Thr Val Ser Val Met Ser Met Ala Leu Asp Phe Asn Glu Ala Phe Thr
10 15 20
gaa cgc acc ccg cgc atc gtc aac gca gct aaa ctg cat cgc gcc gcg 211
Glu Arg Thr Pro Arg Ile Val Asn Ala Ala Lys Leu His Arg Ala Ala
25 30 35
cag cgc aaa aaa gat aag cgt ttt cta gtt gaa ggc gaa aat tcc gtt 259
Gln Arg Lys Lys Asp Lys Arg Phe Leu Val Glu Gly Glu Asn Ser Val
40 45 50
gaa gca gct gtc gct acc ggc gca gca act gac ctc ttt gtc act gaa 307
Glu Ala Ala Val Ala Thr Gly Ala Ala Thr Asp Leu Phe Val Thr Glu
55 60 65
tcc gct gcg gag cgc ttc gag gaa atc gtc cgc acc gcc ggt tac atg 355
Ser Ala Ala Glu Arg Phe Glu Glu Ile Val Arg Thr Ala Gly Tyr Met
70 75 80 85
aat gtc tac acc cac gcg atc acg gac aag gcc gcg aag cat ctt agc 403
Asn Val Tyr Thr His Ala Ile Thr Asp Lys Ala Ala Lys His Leu Ser
90 95 100
gac acc gtc acc acc acg ggc att ttt gcg ctt tgc gac gac gtc ctg 451
Asp Thr Val Thr Thr Thr Gly Ile Phe Ala Leu Cys Asp Asp Val Leu
105 110 115
tgg tca gtc ggc aag gcg atc acc ggc cag cca cgt cta gtg agc gtg 499
Trp Ser Val Gly Lys Ala Ile Thr Gly Gln Pro Arg Leu Val Ser Val
120 125 130
ccg gtt gag acc cgc gag ccc ggt aat gcc gga acc ttg att cgc gta 547
Pro Val Glu Thr Arg Glu Pro Gly Asn Ala Gly Thr Leu Ile Arg Val
135 140 145
tcc gac gcg gtc ggc gcc gac gcc gtc gtc ttc gct ggt gaa tca gta 595
Ser Asp Ala Val Gly Ala Asp Ala Val Val Phe Ala Gly Glu Ser Val
150 155 160 165
gat cca ctt ggc gca aaa gct gtg cgc tcc tca gcg gga tcg ctg ttt 643
Asp Pro Leu Gly Ala Lys Ala Val Arg Ser Ser Ala Gly Ser Leu Phe
170 175 180
cac att cca gtg gca cgc aac aac aac atc gca gat gtc ttg ggg cag 691
His Ile Pro Val Ala Arg Asn Asn Asn Ile Ala Asp Val Leu Gly Gln
185 190 195
ctt cgt tcc aag ggt ctg cag atc ctt gcg acc tca gcc gat ggg gaa 739
Leu Arg Ser Lys Gly Leu Gln Ile Leu Ala Thr Ser Ala Asp Gly Glu
200 205 210
gta gac ctc gat gac gcc gat gag ctg cta gcc aag cca acc gca tgg 787
Val Asp Leu Asp Asp Ala Asp Glu Leu Leu Ala Lys Pro Thr Ala Trp
215 220 225
ctt ttt ggt aat gaa gct cac gga ctt gat gag agc ctg ctt gct cag 835
Leu Phe Gly Asn Glu Ala His Gly Leu Asp Glu Ser Leu Leu Ala Gln
230 235 240 245
gct gat cac cgc gtg cgt att ccg atc cgc ggc cgc gca gaa tca ctc 883
Ala Asp His Arg Val Arg Ile Pro Ile Arg Gly Arg Ala Glu Ser Leu
250 255 260
aat ttg gcc aca gca gcg tca att tgt ctg tac gaa tcc tcc aag gca 931
Asn Leu Ala Thr Ala Ala Ser Ile Cys Leu Tyr Glu Ser Ser Lys Ala
265 270 275
cta ttc gcc ggt gag taaaccccaa ttcatgccca gcgggtatga 976
Leu Phe Ala Gly Glu
280
<210>34
<211>282
<212>PRT
<213>谷氨酸棒状杆菌
<400>34
Leu Thr Ala Arg Leu Thr Val Ser Val Met Ser Met Ala Leu Asp Phe
1 5 10 15
Asn Glu Ala Phe Thr Glu Arg Thr Pro Arg Ile Val Asn Ala Ala Lys
20 25 30
Leu His Arg Ala Ala Gln Arg Lys Lys Asp Lys Arg Phe Leu Val Glu
35 40 45
Gly Glu Asn Ser Val Glu Ala Ala Val Ala Thr Gly Ala Ala Thr Asp
50 55 60
Leu Phe Val Thr Glu Ser Ala Ala Glu Arg Phe Glu Glu Ile Val Arg
65 70 75 80
Thr Ala Gly Tyr Met Asn Val Tyr Thr His Ala Ile Thr Asp Lys Ala
85 90 95
Ala Lys His Leu Ser Asp Thr Val Thr Thr Thr Gly Ile Phe Ala Leu
100 105 110
Cys Asp Asp Val Leu Trp Ser Val Gly Lys Ala Ile Thr Gly Gln Pro
115 120 125
Arg Leu Val Ser Val Pro Val Glu Thr Arg Glu Pro Gly Asn Ala Gly
130 135 140
Thr Leu Ile Arg Val Ser Asp Ala Val Gly Ala Asp Ala Val Val Phe
145 150 155 160
Ala Gly Glu Ser Val Asp Pro Leu Gly Ala Lys Ala Val Arg Ser Ser
165 170 175
Ala Gly Ser Leu Phe His Ile Pro Val Ala Arg Asn Asn Asn Ile Ala
180 185 190
Asp Val Leu Gly Gln Leu Arg Ser Lys Gly Leu Gln Ile Leu Ala Thr
195 200 205
Ser Ala Asp Gly Glu Val Asp Leu Asp Asp Ala Asp Glu Leu Leu Ala
210 215 220
Lys Pro Thr Ala Trp Leu Phe Gly Asn Glu Ala His Gly Leu Asp Glu
225 230 235 240
Ser Leu Leu Ala Gln Ala Asp His Arg Val Arg Ile Pro Ile Arg Gly
245 250 255
Arg Ala Glu Ser Leu Asn Leu Ala Thr Ala Ala Ser Ile Cys Leu Tyr
260 265 270
Glu Ser Ser Lys Ala Leu Phe Ala Gly Glu
275 280
<210>35
<211>853
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(823)
<223>RXA01661
<400>35
accaccggaa attcccctct gacagctgtt gccgcgttgc gtgaagctgg cattgaggtt 60
gtgggcgttg ccaccgtggt cgatcgcgca accggtgcag atg agg tta tcg cag 115
Met Arg Leu Ser Gln
1 5
cgg aag gcc ttc ctt acc gca gct tgc tgg gac ttt ctg atc ttg gac 163
Arg Lys Ala Phe Leu Thr Ala Ala Cys Trp Asp Phe Leu Ile Leu Asp
10 15 20
tca act aac acc ccc ggc ccc acg gag tgg ggc gag tcc cgc gtg ggc 211
Ser Thr Asn Thr Pro Gly Pro Thr Glu Trp Gly Glu Ser Arg Val Gly
25 30 35
aaa ggt cca tgg gaa gag gaa aac ccc ggc gta cct cgg cct aca agc 259
Lys Gly Pro Trp Glu Glu Glu Asn Pro Gly Val Pro Arg Pro Thr Ser
40 45 50
ccg ctt ttc gac gtc acc ctc ctc aac gag ggc gat cgc cgc aac gtt 307
Pro Leu Phe Asp Val Thr Leu Leu Asn Glu Gly Asp Arg Arg Asn Val
55 60 65
gtt gac gcc tat cgt tat tgg acc cgt gag gcg att gtt gaa gat atc 355
Val Asp Ala Tyr Arg Tyr Trp Thr Arg Glu Ala Ile Val Glu Asp Ile
70 75 80 85
gac acc cgc cgc cac agc ctc cac gta gcg atc gaa aac ttt gaa aac 403
Asp Thr Arg Arg His Ser Leu His Val Ala Ile Glu Asn Phe Glu Asn
90 95 100
gac gcc aac atc ggc acc gtc gtg cgc acc gcc aac gcc ttt gcc gtg 451
Asp Ala Asn Ile Gly Thr Val Val Arg Thr Ala Asn Ala Phe Ala Val
105 110 115
aac aca gtc cac att gtg ggc agg cgt cgg tgg aac cgc agg gga gcc 499
Asn Thr Val His Ile Val Gly Arg Arg Arg Trp Asn Arg Arg Gly Ala
120 125 130
atg gtg act gac cgt tac cag cac ctc atg cac cac gaa gac gtt gat 547
Met Val Thr Asp Arg Tyr Gln His Leu Met His His Glu Asp Val Asp
135 140 145
tcg ctg ctt gcg tgg gca atc gcg gag cgg ctt acc atc gtc gcg atc 595
Ser Leu Leu Ala Trp Ala Ile Ala Glu Arg Leu Thr Ile Val Ala Ile
150 155 160 165
gat aac acc cca ggt tcc gtg cct ttg gaa acc gct gag ttg ccg aag 643
Asp Asn Thr Pro Gly Ser Val Pro Leu Glu Thr Ala Glu Leu Pro Lys
170 175 180
aac tgc ctg ttg ctg ttt ggt cag gaa ggc cca ggt gtc acc gaa gct 691
Asn Cys Leu Leu Leu Phe Gly Gln Glu Gly Pro Gly Val Thr Glu Ala
185 190 195
gcg cgt gca ggt gcg tta atg acc tgt tcg att gcc caa ttt ggt tcc 739
Ala Arg Ala Gly Ala Leu Met Thr Cys Ser Ile Ala Gln Phe Gly Ser
200 205 210
act cga tcc atc aac gcg ggt gta gct gct ggt att gca atg cat gca 787
Thr Arg Ser Ile Asn Ala Gly Val Ala Ala Gly Ile Ala Met His Ala
215 220 225
tgg att cgt caa cat gcc gac tta tcg caa gcc tgg taattttata 833
Trp Ile Arg Gln His Ala Asp Leu Ser Gln Ala Trp
230 235 240
ccctagatcg ttagactttc 853
<210>36
<211>241
<212>PRT
<213>谷氨酸棒状杆菌
<400>36
Met Arg Leu Ser Gln Arg Lys Ala Phe Leu Thr Ala Ala Cys Trp Asp
1 5 10 15
Phe Leu Ile Leu Asp Ser Thr Asn Thr Pro Gly Pro Thr Glu Trp Gly
20 25 30
Glu Ser Arg Val Gly Lys Gly Pro Trp Glu Glu Glu Asn Pro Gly Val
35 40 45
Pro Arg Pro Thr Ser Pro Leu Phe Asp Val Thr Leu Leu Asn Glu Gly
50 55 60
Asp Arg Arg Asn Val Val Asp Ala Tyr Arg Tyr Trp Thr Arg Glu Ala
65 70 75 80
Ile Val Glu Asp Ile Asp Thr Arg Arg His Ser Leu His Val Ala Ile
85 90 95
Glu Asn Phe Glu Asn Asp Ala Asn Ile Gly Thr Val Val Arg Thr Ala
100 105 110
Asn Ala Phe Ala Val Asn Thr Val His Ile Val Gly Arg Arg Arg Trp
115 120 125
Asn Arg Arg Gly Ala Met Val Thr Asp Arg Tyr Gln His Leu Met His
130 135 140
His Glu Asp Val Asp Ser Leu Leu Ala Trp Ala Ile Ala Glu Arg Leu
145 150 155 160
Thr Ile Val Ala Ile Asp Asn Thr Pro Gly Ser Val Pro Leu Glu Thr
165 170 175
Ala Glu Leu Pro Lys Asn Cys Leu Leu Leu Phe Gly Gln Glu Gly Pro
180 185 190
Gly Val Thr Glu Ala Ala Arg Ala Gly Ala Leu Met Thr Cys Ser Ile
195 200 205
Ala Gln Phe Gly Ser Thr Arg Ser Ile Asn Ala Gly Val Ala Ala Gly
210 215 220
Ile Ala Met His Ala Trp Ile Arg Gln His Ala Asp Leu Ser Gln Ala
225 230 235 240
Trp
<210>37
<211>2698
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2668)
<223>RXA01683
<400>37
cccaaaatgg tatcgctttg ataccaattt tggggcatgc aaggcgttaa aacggcctgt 60
ggactaaaat cggtagggtt gacacgaaga aaggtgatca gtg agc gac gac aat 115
Val Ser Asp Asp Asn
1 5
acc gga caa ttt gac cgc gtt aat ccc att gat atc aat gag gaa atg 163
Thr Gly Gln Phe Asp Arg Val Asn Pro Ile Asp Ile Asn Glu Glu Met
10 15 20
cag tcg agc tac atc gac tac gcg atg tca gtc atc gtc gga cgt gcc 211
Gln Ser Ser Tyr Ile Asp Tyr Ala Met Ser Val Ile Val Gly Arg Ala
25 30 35
ctc cca gag gtg cgc gac ggc ctg aag cca gtc cac cgc cgc gtc ttg 259
Leu Pro Glu Val Arg Asp Gly Leu Lys Pro Val His Arg Arg Val Leu
40 45 50
tac gcg atg ttc gac aac ggc tac cgc ccc gac cgc agc tac gtg aag 307
Tyr Ala Met Phe Asp Asn Gly Tyr Arg Pro Asp Arg Ser Tyr Val Lys
55 60 65
tct gca aaa cca gtg gca gac acc atg ggt aac ttc cac cca cac ggc 355
Ser Ala Lys Pro Val Ala Asp Thr Met Gly Asn Phe His Pro His Gly
70 75 80 85
gac acc gca att tat gac acg ttg gtg cgc atg gct cag cca tgg tcc 403
Asp Thr Ala Ile Tyr Asp Thr Leu Val Arg Met Ala Gln Pro Trp Ser
90 95 100
atg cga tac ccg ctg gta gac ggc cag ggt aac ttc ggt tcc cgc ggc 451
Met Arg Tyr Pro Leu Val Asp Gly Gln Gly Asn Phe Gly Ser Arg Gly
105 110 115
aac gac ggc cct gca gca atg cgt tac acc gag tgc cgc atg acc cca 499
Asn Asp Gly Pro Ala Ala Met Arg Tyr Thr Glu Cys Arg Met Thr Pro
120 125 130
ctg gcc atg gag atg gtg cgc gac atc cgc gaa aac acc gtc aac ttc 547
Leu Ala Met Glu Met Val Arg Asp Ile Arg Glu Asn Thr Val Asn Phe
135 140 145
tca cca aac tac gac ggt aaa acc ctc gaa cca gac gtt ttg cca tcg 595
Ser Pro Asn Tyr Asp Gly Lys Thr Leu Glu Pro Asp Val Leu Pro Ser
150 155 160 165
cgc gtt cca aac ttg ttg atg aac ggt tcg ggc ggc att gcg gtc ggc 643
Arg Val Pro Asn Leu Leu Met Asn Gly Ser Gly Gly Ile Ala Val Gly
170 175 180
atg gcc acc aac atc cca ccg cac aac ctc aac gag ctt gcc gac gcc 691
Met Ala Thr Asn Ile Pro Pro His Asn Leu Asn Glu Leu Ala Asp Ala
185 190 195
atc ttc tgg ctc ctg gaa aac cca gac gcc gaa gaa tcc gaa gct ctc 739
Ile Phe Trp Leu Leu Glu Asn Pro Asp Ala Glu Glu Ser Glu Ala Leu
200 205 210
gaa gcc tgc atg aag ttt gtg aag ggc cca gac ttc cca acc gct ggc 787
Glu Ala Cys Met Lys Phe Val Lys Gly Pro Asp Phe Pro Thr Ala Gly
215 220 225
ctc atc atc ggt gac aag ggc atc cac gat gcc tac acc acc ggc cgc 835
Leu Ile Ile Gly Asp Lys Gly Ile His Asp Ala Tyr Thr Thr Gly Arg
230 235 240 245
ggc tcc atc cgc atg cgc ggt gtc acc tcc atc gag gag gaa ggc aac 883
Gly Ser Ile Arg Met Arg Gly Val Thr Ser Ile Glu Glu Glu Gly Asn
250 255 260
cgc acc gtc atc gtt atc acc gag ctg cca tac cag gtc aac ccg gat 931
Arg Thr Val Ile Val Ile Thr Glu Leu Pro Tyr Gln Val Asn Pro Asp
265 270 275
aac ctg atc tct aat atc gcg gag cag gtg cgc gac ggc aag ctc gtg 979
Asn Leu Ile Ser Asn Ile Ala Glu Gln Val Arg Asp Gly Lys Leu Val
280 285 290
ggc atc tcc aag att gaa gat gaa tcc tcc gac cgc gtc ggc atg cgc 1027
Gly Ile Ser Lys Ile Glu Asp Glu Ser Ser Asp Arg Val Gly Met Arg
295 300 305
att gtg gtc acc ctc aag cgc gac gca gtt gcc cgc gtg gtg ctg aac 1075
Ile Val Val Thr Leu Lys Arg Asp Ala Val Ala Arg Val Val Leu Asn
310 315 320 325
aac ctg ttc aag cac tcc cag ctg caa gcc aac ttt ggt gcg aac atg 1123
Asn Leu Phe Lys His Ser Gln Leu Gln Ala Asn Phe Gly Ala Asn Met
330 335 340
ctc tcc atc gtc gat ggc gtg cca cgc acc ctt cgc ctg gac cag atg 1171
Leu Ser Ile Val Asp Gly Val Pro Arg Thr Leu Arg Leu Asp Gln Met
345 350 355
ctg cgc tac tac gtg gca cac cag atc gaa gtc atc gtg cgc cgc acc 1219
Leu Arg Tyr Tyr Val Ala His Gln Ile Glu Val Ile Val Arg Arg Thr
360 365 370
caa tac cgc ctc gac aag gct gaa gag cgc gcc cac ctc ctc cgc ggc 1267
Gln Tyr Arg Leu Asp Lys Ala Glu Glu Arg Ala His Leu Leu Arg Gly
375 380 385
ctg gtc aag gcc ctg gac atg ctg gac gag gtc atc gcg ctc atc cgc 1315
Leu Val Lys Ala Leu Asp Met Leu Asp Glu Val Ile Ala Leu Ile Arg
390 395 400 405
cgc agc cca acc cca gat gaa gcc cgc acc ggc ctc atg tcg ctt ctc 1363
Arg Ser Pro Thr Pro Asp Glu Ala Arg Thr Gly Leu Met Ser Leu Leu
410 415 420
gac gtc gac gag gcg cag gct gac gca att ctg gca atg cag ctg cgt 1411
Asp Val Asp Glu Ala Gln Ala Asp Ala Ile Leu Ala Met Gln Leu Arg
425 430 435
cgc ctg gcg gca ctg gaa cgc caa aag atc atc gat gag ctc gct gaa 1459
Arg Leu Ala Ala Leu Glu Arg Gln Lys Ile Ile Asp Glu Leu Ala Glu
440 445 450
atc gag ctg gaa atc gct gac ctg aag gcc atc ctg gca agc cca gaa 1507
Ile Glu Leu Glu Ile Ala Asp Leu Lys Ala Ile Leu Ala Ser Pro Glu
455 460 465
cgt cag cgc acc atc gtt cgc gat gag ctg acc gaa atc gtg gaa aag 1555
Arg Gln Arg Thr Ile Val Arg Asp Glu Leu Thr Glu Ile Val Glu Lys
470 475 480 485
tac ggc gac gag cgt cgt tcc cag atc atc gct gcc acc ggc gac gtg 1603
Tyr Gly Asp Glu Arg Arg Ser Gln Ile Ile Ala Ala Thr Gly Asp Val
490 495 500
tct gaa gaa gac ctc att gcg cgt gaa aac gtt gtc atc acc att acc 1651
Ser Glu Glu Asp Leu Ile Ala Arg Glu Asn Val Val Ile Thr Ile Thr
505 510 515
tcc acc ggt tac gca aag cgc acc aag gtc gat gcc tac aag tcg caa 1699
Ser Thr Gly Tyr Ala Lys Arg Thr Lys Val Asp Ala Tyr Lys Ser Gln
520 525 530
aag cgt ggc ggc aag ggt gtt cgt ggc gca gag ctc aag caa gat gac 1747
Lys Arg Gly Gly Lys Gly Val Arg Gly Ala Glu Leu Lys Gln Asp Asp
535 540 545
att gtt cgt cac ttc ttc gtc agc tcc acc cac gac tgg att ttg ttc 1795
Ile Val Arg His Phe Phe Val Ser Ser Thr His Asp Trp Ile Leu Phe
550 555 560 565
ttc acc aac tac ggt cgc gtg tac cgc ctc aag gca ttc gaa ctt cca 1843
Phe Thr Asn Tyr Gly Arg Val Tyr Arg Leu Lys Ala Phe Glu Leu Pro
570 575 580
gag gca tcc cgc acc gca cgt gga cag cac gtg gcc aac ctt ctg gaa 1891
Glu Ala Ser Arg Thr Ala Arg Gly Gln His Val Ala Asn Leu Leu Glu
585 590 595
ttc caa cct ggt gag caa atc gcc cag gtc atc cag ttg gaa agc tac 1939
Phe Gln Pro Gly Glu Gln Ile Ala Gln Val Ile Gln Leu Glu Ser Tyr
600 605 610
aac gac ttc cca tac ctg gtg ctc gca acc gca cac ggt cgc gtg aag 1987
Asn Asp Phe Pro Tyr Leu Val Leu Ala Thr Ala His Gly Arg Val Lys
615 620 625
aag tcc cgc ctg ctc gac tac gaa tca gca cgt tcc ggt ggc ctc atc 2035
Lys Ser Arg Leu Leu Asp Tyr Glu Ser Ala Arg Ser Gly Gly Leu Ile
630 635 640 645
gcc atc aac ctg aac gag gac gat cgc ctc atc ggc gcc gca ctt tgc 2083
Ala Ile Asn Leu Asn Glu Asp Asp Arg Leu Ile Gly Ala Ala Leu Cys
650 655 660
ggt gaa gaa gac gat ctg ctg ctg gtc tct gaa ttc gga cag tcc atc 2131
Gly Glu Glu Asp Asp Leu Leu Leu Val Ser Glu Phe Gly Gln Ser Ile
665 670 675
cgc ttc acc gcc gac gat gag cag ctc cgc ccc atg ggc cgc gcc acc 2179
Arg Phe Thr Ala Asp Asp Glu Gln Leu Arg Pro Met Gly Arg Ala Thr
680 685 690
gcc ggt gtc aag ggc atg cgc ttc cgc gac aac gac caa ctg ctg tcc 2227
Ala Gly Val Lys Gly Met Arg Phe Arg Asp Asn Asp Gln Leu Leu Ser
695 700 705
atg tcc gtg gtc cgc gac ggc gaa ttc ctc ctc gtt gcc acc tcc ggc 2275
Met Ser Val Val Arg Asp Gly Glu Phe Leu Leu Val Ala Thr Ser Gly
710 715 720 725
ggc tac ggc aag cgc acc cca ctt gag gat tac tcc acc cag ggc cgt 2323
Gly Tyr Gly Lys Arg Thr Pro Leu Glu Asp Tyr Ser Thr Gln Gly Arg
730 735 740
ggt ggc ctc ggc gtg gtg acc ttc aag tac acc ccg aag cgc ggt cgc 2371
Gly Gly Leu Gly Val Val Thr Phe Lys Tyr Thr Pro Lys Arg Gly Arg
745 750 755
ctc gtc agc gcc atc gca gtt gag gaa gat gac gag atc ttc gcc atc 2419
Leu Val Ser Ala Ile Ala Val Glu Glu Asp Asp Glu Ile Phe Ala Ile
760 765 770
acc tcc gcc ggc ggc gtt gtt cgc acc gaa gtc aag cag atc cga cca 2467
Thr Ser Ala Gly Gly Val Val Arg Thr Glu Val Lys Gln Ile Arg Pro
775 780 785
tcc tcc cgt gca aca atg ggt gtt cga ctg gtc aac ttg gaa gaa ggt 2515
Ser Ser Arg Ala Thr Met Gly Val Arg Leu Val Asn Leu Glu Glu Gly
790 795 800 805
gta gaa ctg ctt gcc atc gac aag aac gtc gaa gac cag ggc gaa gca 2563
Val Glu Leu Leu Ala Ile Asp Lys Asn Val Glu Asp Gln Gly Glu Ala
810 815 820
tcc gca gaa gca gta gca aag ggt gca gtc gaa gga cca gca tcc aag 2611
Ser Ala Glu Ala Val Ala Lys Gly Ala Val Glu Gly Pro Ala Ser Lys
825 830 835
act gct gcc gaa gaa acc gac tcc gtt gac aac gga tcc gac gaa aac 2659
Thr Ala Ala Glu Glu Thr Asp Ser Val Asp Asn Gly Ser Asp Glu Asn
840 845 850
ggc gag gaa taatttatgg catcccgaga agtatccatc 2698
Gly Glu Glu
855
<210>38
<211>856
<212>PRT
<213>谷氨酸棒状杆菌
<400>38
Val Ser Asp Asp Asn Thr Gly Gln Phe Asp Arg Val Asn Pro Ile Asp
1 5 10 15
Ile Asn Glu Glu Met Gln Ser Ser Tyr Ile Asp Tyr Ala Met Ser Val
20 25 30
Ile Val Gly Arg Ala Leu Pro Glu Val Arg Asp Gly Leu Lys Pro Val
35 40 45
His Arg Arg Val Leu Tyr Ala Met Phe Asp Asn Gly Tyr Arg Pro Asp
50 55 60
Arg Ser Tyr Val Lys Ser Ala Lys Pro Val Ala Asp Thr Met Gly Asn
65 70 75 80
Phe His Pro His Gly Asp Thr Ala Ile Tyr Asp Thr Leu Val Arg Met
85 90 95
Ala Gln Pro Trp Ser Met Arg Tyr Pro Leu Val Asp Gly Gln Gly Asn
100 105 110
Phe Gly Ser Arg Gly Asn Asp Gly Pro Ala Ala Met Arg Tyr Thr Glu
115 120 125
Cys Arg Met Thr Pro Leu Ala Met Glu Met Val Arg Asp Ile Arg Glu
130 135 140
Asn Thr Val Asn Phe Ser Pro Asn Tyr Asp Gly Lys Thr Leu Glu Pro
145 150 155 160
Asp Val Leu Pro Ser Arg Val Pro Asn Leu Leu Met Asn Gly Ser Gly
165 170 175
Gly Ile Ala Val Gly Met Ala Thr Asn Ile Pro Pro His Asn Leu Asn
180 185 190
Glu Leu Ala Asp Ala Ile Phe Trp Leu Leu Glu Asn Pro Asp Ala Glu
195 200 205
Glu Ser Glu Ala Leu Glu Ala Cys Met Lys Phe Val Lys Gly Pro Asp
210 215 220
Phe Pro Thr Ala Gly Leu Ile Ile Gly Asp Lys Gly Ile His Asp Ala
225 230 235 240
Tyr Thr Thr Gly Arg Gly Ser Ile Arg Met Arg Gly Val Thr Ser Ile
245 250 255
Glu Glu Glu Gly Asn Arg Thr Val Ile Val Ile Thr Glu Leu Pro Tyr
260 265 270
Gln Val Asn Pro Asp Asn Leu Ile Ser Asn Ile Ala Glu Gln Val Arg
275 280 285
Asp Gly Lys Leu Val Gly Ile Ser Lys Ile Glu Asp Glu Ser Ser Asp
290 295 300
Arg Val Gly Met Arg Ile Val Val Thr Leu Lys Arg Asp Ala Val Ala
305 310 315 320
Arg Val Val Leu Asn Asn Leu Phe Lys His Ser Gln Leu Gln Ala Asn
325 330 335
Phe Gly Ala Asn Met Leu Ser Ile Val Asp Gly Val Pro Arg Thr Leu
340 345 350
Arg Leu Asp Gln Met Leu Arg Tyr Tyr Val Ala His Gln Ile Glu Val
355 360 365
Ile Val Arg Arg Thr Gln Tyr Arg Leu Asp Lys Ala Glu Glu Arg Ala
370 375 380
His Leu Leu Arg Gly Leu Val Lys Ala Leu Asp Met Leu Asp Glu Val
385 390 395 400
Ile Ala Leu Ile Arg Arg Ser Pro Thr Pro Asp Glu Ala Arg Thr Gly
405 410 415
Leu Met Ser Leu Leu Asp Val Asp Glu Ala Gln Ala Asp Ala Ile Leu
420 425 430
Ala Met Gln Leu Arg Arg Leu Ala Ala Leu Glu Arg Gln Lys Ile Ile
435 440 445
Asp Glu Leu Ala Glu Ile Glu Leu Glu Ile Ala Asp Leu Lys Ala Ile
450 455 460
Leu Ala Ser Pro Glu Arg Gln Arg Thr Ile Val Arg Asp Glu Leu Thr
465 470 475 480
Glu Ile Val Glu Lys Tyr Gly Asp Glu Arg Arg Ser Gln Ile Ile Ala
485 490 495
Ala Thr Gly Asp Val Ser Glu Glu Asp Leu Ile Ala Arg Glu Asn Val
500 505 510
Val Ile Thr Ile Thr Ser Thr Gly Tyr Ala Lys Arg Thr Lys Val Asp
515 520 525
Ala Tyr Lys Ser Gln Lys Arg Gly Gly Lys Gly Val Arg Gly Ala Glu
530 535 540
Leu Lys Gln Asp Asp Ile Val Arg His Phe Phe Val Ser Ser Thr His
545 550 555 560
Asp Trp Ile Leu Phe Phe Thr Asn Tyr Gly Arg Val Tyr Arg Leu Lys
565 570 575
Ala Phe Glu Leu Pro Glu Ala Ser Arg Thr Ala Arg Gly Gln His Val
580 585 590
Ala Asn Leu Leu Glu Phe Gln Pro Gly Glu Gln Ile Ala Gln Val Ile
595 600 605
Gln Leu Glu Ser Tyr Asn Asp Phe Pro Tyr Leu Val Leu Ala Thr Ala
610 615 620
His Gly Arg Val Lys Lys Ser Arg Leu Leu Asp Tyr Glu Ser Ala Arg
625 630 635 640
Ser Gly Gly Leu Ile Ala Ile Asn Leu Asn Glu Asp Asp Arg Leu Ile
645 650 655
Gly Ala Ala Leu Cys Gly Glu Glu Asp Asp Leu Leu Leu Val Ser Glu
660 665 670
Phe Gly Gln Ser Ile Arg Phe Thr Ala Asp Asp Glu Gln Leu Arg Pro
675 680 685
Met Gly Arg Ala Thr Ala Gly Val Lys Gly Met Arg Phe Arg Asp Asn
690 695 700
Asp Gln Leu Leu Ser Met Ser Val Val Arg Asp Gly Glu Phe Leu Leu
705 710 715 720
Val Ala Thr Ser Gly Gly Tyr Gly Lys Arg Thr Pro Leu Glu Asp Tyr
725 730 735
Ser Thr Gln Gly Arg Gly Gly Leu Gly Val Val Thr Phe Lys Tyr Thr
740 745 750
Pro Lys Arg Gly Arg Leu Val Ser Ala Ile Ala Val Glu Glu Asp Asp
755 760 765
Glu Ile Phe Ala Ile Thr Ser Ala Gly Gly Val Val Arg Thr Glu Val
770 775 780
Lys Gln Ile Arg Pro Ser Ser Arg Ala Thr Met Gly Val Arg Leu Val
785 790 795 800
Asn Leu Glu Glu Gly Val Glu Leu Leu Ala Ile Asp Lys Asn Val Glu
805 810 815
Asp Gln Gly Glu Ala Ser Ala Glu Ala Val Ala Lys Gly Ala Val Glu
820 825 830
Gly Pro Ala Ser Lys Thr Ala Ala Glu Glu Thr Asp Ser Val Asp Asn
835 840 845
Gly Ser Asp Glu Asn Gly Glu Glu
850 855
<210>39
<211>1483
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1453)
<223>RXA01718
<400>39
gatctatctg gcctgtccac actaatctat ttgtgttgag gcaggtggaa tcttgttaac 60
agctgttttg tctttaattg ctggtttggt ggtcattggg gtg atc atc gtc ctc 115
Val Ile Ile Val Leu
1 5
aac gga tat ttt gtg gct cag gaa ttt gcc tac atg tcc gtt gat cga 163
Asn Gly Tyr Phe Val Ala Gln Glu Phe Ala Tyr Met Ser Val Asp Arg
10 15 20
aat gag ctg cga gct ctc gct gac tct gga gat aag aag gct cgt cgc 211
Asn Glu Leu Arg Ala Leu Ala Asp Ser Gly Asp Lys Lys Ala Arg Arg
25 30 35
gct ctc agc atc act aag cgc aca tcc ttt atg ctt tct ggt gcg caa 259
Ala Leu Ser Ile Thr Lys Arg Thr Ser Phe Met Leu Ser Gly Ala Gln
40 45 50
ttg ggt atc acc gtc acc gga ctt ttg gtg ggt ttt gtg gcc gaa cct 307
Leu Gly Ile Thr Val Thr Gly Leu Leu Val Gly Phe Val Ala Glu Pro
55 60 65
ctg gtg ggt aac gcg tta ggt gtt ctg cta gga ggg gtg ggg gtt ccc 355
Leu Val Gly Asn Ala Leu Gly Val Leu Leu Gly Gly Val Gly Val Pro
70 75 80 85
gct gcg gtg tca atc tct gtg ggc act gtg ttg gcg cta gcg atc tcc 403
Ala Ala Val Ser Ile Ser Val Gly Thr Val Leu Ala Leu Ala Ile Ser
90 95 100
acg gtt gtc caa atg att ttt ggt gaa ctc ttc cct aag aac tac acc 451
Thr Val Val Gln Met Ile Phe Gly Glu Leu Phe Pro Lys Asn Tyr Thr
105 110 115
ctt gcc acg ccg ttg aag tct gcg ctg gcg ctt gcg cct tcg acc acg 499
Leu Ala Thr Pro Leu Lys Ser Ala Leu Ala Leu Ala Pro Ser Thr Thr
120 125 130
tgg tat ttg aaa ctc gct ggt tgg ttg atc aca ttc ttt gat ttt gca 547
Trp Tyr Leu Lys Leu Ala Gly Trp Leu Ile Thr Phe Phe Asp Phe Ala
135 140 145
tcc aac gca ttg ctt cga ctc ttt cga ata gag ccg gtg gaa gat gtt 595
Ser Asn Ala Leu Leu Arg Leu Phe Arg Ile Glu Pro Val Glu Asp Val
150 155 160 165
gat tcg tct gcc act gca caa gac ctt ccg cat att gtg gcg tca tct 643
Asp Ser Ser Ala Thr Ala Gln Asp Leu Pro His Ile Val Ala Ser Ser
170 175 180
agg gat agt ggc gtt tta aat aat tcg atg tct ttg tcg ctt gat cga 691
Arg Asp Ser Gly Val Leu Asn Asn Ser Met Ser Leu Ser Leu Asp Arg
185 190 195
ttg ttg gat ttc ccg tcg cat gat gtg ggt cat gca atg att ccg cgt 739
Leu Leu Asp Phe Pro Ser His Asp Val Gly His Ala Met Ile Pro Arg
200 205 210
tcg cgt gtg ggt gtg gtt gat ccg gag aca acc atc gct gag gtt aag 787
Ser Arg Val Gly Val Val Asp Pro Glu Thr Thr Ile Ala Glu Val Lys
215 220 225
gcc ctc atg agg aaa gcg cat acg cgt tac ccc atc atc gat gac aac 835
Ala Leu Met Arg Lys Ala His Thr Arg Tyr Pro Ile Ile Asp Asp Asn
230 235 240 245
cat gtg ccc att ggg gtg atc aat ctg att gat att ctt gga acc gat 883
His Val Pro Ile Gly Val Ile Asn Leu Ile Asp Ile Leu Gly Thr Asp
250 255 260
att cac ggc gcc gaa att tca gat tcc gcg aaa gtc act gag ttc atg 931
Ile His Gly Ala Glu Ile Ser Asp Ser Ala Lys Val Thr Glu Phe Met
265 270 275
cat cag cct gtg atc gtt cca gag ttt atg tct ttg cct gac gtg gtg 979
His Gln Pro Val Ile Val Pro Glu Phe Met Ser Leu Pro Asp Val Val
280 285 290
acc gaa ctg cac aat cgt gaa gac agg ctt gct tgc gtg att gat gaa 1027
Thr Glu Leu His Asn Arg Glu Asp Arg Leu Ala Cys Val Ile Asp Glu
295 300 305
tac ggt gga ttc att ggc att gtg aca tta gaa gat tta gct gag gaa 1075
Tyr Gly Gly Phe Ile Gly Ile Val Thr Leu Glu Asp Leu Ala Glu Glu
310 315 320 325
gtc ctc ggc gat atc aac gat gag cac gat gtt ttc agc tct gaa gat 1123
Val Leu Gly Asp Ile Asn Asp Glu His Asp Val Phe Ser Ser Glu Asp
330 335 340
att acg gaa acg agc cct gac aaa tgg cta att gat ggc gat acc cct 1171
Ile Thr Glu Thr Ser Pro Asp Lys Trp Leu Ile Asp Gly Asp Thr Pro
345 350 355
ctt gac gag gtt gag cgc gcc atc ggg tac gag cta cct gaa ggc gat 1219
Leu Asp Glu Val Glu Arg Ala Ile Gly Tyr Glu Leu Pro Glu Gly Asp
360 365 370
tac gag acg att tct gga ctg ctc ttt gac cat gcg aat gcc ctt cta 1267
Tyr Glu Thr Ile Ser Gly Leu Leu Phe Asp His Ala Asn Ala Leu Leu
375 380 385
aag act ggg gat gtc ata gaa ata cct ctg gat ttt gag cca gag gat 1315
Lys Thr Gly Asp Val Ile Glu Ile Pro Leu Asp Phe Glu Pro Glu Asp
390 395 400 405
tac ctg aac aac acc tcc cct acg cag cgt att ttg cgc atc act gtt 1363
Tyr Leu Asn Asn Thr Ser Pro Thr Gln Arg Ile Leu Arg Ile Thr Val
410 415 420
tta gag gta gag cga aac gtg cct gta aaa ctg gcg ctc gct ctg ctc 1411
Leu Glu Val Glu Arg Asn Val Pro Val Lys Leu Ala Leu Ala Leu Leu
425 430 435
gaa gac cac cca gac aat cac ccc gct ccg aag gag aat cgc 1453
Glu Asp His Pro Asp Asn His Pro Ala Pro Lys Glu Asn Arg
440 445 450
taagccatga ctgaatggta tgtcgtttta 1483
<210>40
<211>451
<212>PRT
<213>谷氨酸棒状杆菌
<400>40
Val Ile Ile Val Leu Asn Gly Tyr Phe Val Ala Gln Glu Phe Ala Tyr
1 5 10 15
Met Ser Val Asp Arg Asn Glu Leu Arg Ala Leu Ala Asp Ser Gly Asp
20 25 30
Lys Lys Ala Arg Arg Ala Leu Ser Ile Thr Lys Arg Thr Ser Phe Met
35 40 45
Leu Ser Gly Ala Gln Leu Gly Ile Thr Val Thr Gly Leu Leu Val Gly
50 55 60
Phe Val Ala Glu Pro Leu Val Gly Asn Ala Leu Gly Val Leu Leu Gly
65 70 75 80
Gly Val Gly Val Pro Ala Ala Val Ser Ile Ser Val Gly Thr Val Leu
85 90 95
Ala Leu Ala Ile Ser Thr Val Val Gln Met Ile Phe Gly Glu Leu Phe
100 105 110
Pro Lys Asn Tyr Thr Leu Ala Thr Pro Leu Lys Ser Ala Leu Ala Leu
115 120 125
Ala Pro Ser Thr Thr Trp Tyr Leu Lys Leu Ala Gly Trp Leu Ile Thr
130 135 140
Phe Phe Asp Phe Ala Ser Asn Ala Leu Leu Arg Leu Phe Arg Ile Glu
145 150 155 160
Pro Val Glu Asp Val Asp Ser Ser Ala Thr Ala Gln Asp Leu Pro His
165 170 175
Ile Val Ala Ser Ser Arg Asp Ser Gly Val Leu Asn Asn Ser Met Ser
180 185 190
Leu Ser Leu Asp Arg Leu Leu Asp Phe Pro Ser His Asp Val Gly His
195 200 205
Ala Met Ile Pro Arg Ser Arg Val Gly Val Val Asp Pro Glu Thr Thr
210 215 220
Ile Ala Glu Val Lys Ala Leu Met Arg Lys Ala His Thr Arg Tyr Pro
225 230 235 240
Ile Ile Asp Asp Asn His Val Pro Ile Gly Val Ile Asn Leu Ile Asp
245 250 255
Ile Leu Gly Thr Asp Ile His Gly Ala Glu Ile Ser Asp Ser Ala Lys
260 265 270
Val Thr Glu Phe Met His Gln Pro Val Ile Val Pro Glu Phe Met Ser
275 280 285
Leu Pro Asp Val Val Thr Glu Leu His Asn Arg Glu Asp Arg Leu Ala
290 295 300
Cys Val Ile Asp Glu Tyr Gly Gly Phe Ile Gly Ile Val Thr Leu Glu
305 310 315 320
Asp Leu Ala Glu Glu Val Leu Gly Asp Ile Asn Asp Glu His Asp Val
325 330 335
Phe Ser Ser Glu Asp Ile Thr Glu Thr Ser Pro Asp Lys Trp Leu Ile
340 345 350
Asp Gly Asp Thr Pro Leu Asp Glu Val Glu Arg Ala Ile Gly Tyr Glu
355 360 365
Leu Pro Glu Gly Asp Tyr Glu Thr Ile Ser Gly Leu Leu Phe Asp His
370 375 380
Ala Asn Ala Leu Leu Lys Thr Gly Asp Val Ile Glu Ile Pro Leu Asp
385 390 395 400
Phe Glu Pro Glu Asp Tyr Leu Asn Asn Thr Ser Pro Thr Gln Arg Ile
405 410 415
Leu Arg Ile Thr Val Leu Glu Val Glu Arg Asn Val Pro Val Lys Leu
420 425 430
Ala Leu Ala Leu Leu Glu Asp His Pro Asp Asn His Pro Ala Pro Lys
435 440 445
Glu Asn Arg
450
<210>41
<211>3340
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3310)
<223>RXA01736
<400>41
cacctccgcg gccccaatct gctcgcccga gtaaatccca catgctcgac gtgtcccgtt 60
cgaagcctgt gcccagttca acccgaagga cgtgtcatcc atg cgt agg att tct 115
Met Arg Arg Ile Ser
1 5
cct cga ctg ctc tcc caa tat ttg gga caa gag cat gcg cca act gat 163
Pro Arg Leu Leu Ser Gln Tyr Leu Gly Gln Glu His Ala Pro Thr Asp
10 15 20
cag caa tct gcg atc att gga tcc gaa ccc ggc cca ctg ttg gta gtg 211
Gln Gln Ser Ala Ile Ile Gly Ser Glu Pro Gly Pro Leu Leu Val Val
25 30 35
gct ggt gcc ggc gca gga aaa acg gag acg atg gct gca cga gtg gtg 259
Ala Gly Ala Gly Ala Gly Lys Thr Glu Thr Met Ala Ala Arg Val Val
40 45 50
tgg ttg gta gcg aac gga ttc gtc gcc cca gat caa gtg ctg ggt ctg 307
Trp Leu Val Ala Asn Gly Phe Val Ala Pro Asp Gln Val Leu Gly Leu
55 60 65
aca ttt acc agg aag gcg gcg caa cag ctg tcg cag cgc atc cga aag 355
Thr Phe Thr Arg Lys Ala Ala Gln Gln Leu Ser Gln Arg Ile Arg Lys
70 75 80 85
cgt ttg gag act ctt gct ggt gtg cct cgc ctg aaa gac att gat ccc 403
Arg Leu Glu Thr Leu Ala Gly Val Pro Arg Leu Lys Asp Ile Asp Pro
90 95 100
acc ggc cag ttg gag aaa aac ctt cag gcg atc acc cca acg gta tcc 451
Thr Gly Gln Leu Glu Lys Asn Leu Gln Ala Ile Thr Pro Thr Val Ser
105 110 115
acc tat gac tcc tat gcg ggc acg ttg att aga gaa tac gga ttg ctg 499
Thr Tyr Asp Ser Tyr Ala Gly Thr Leu Ile Arg Glu Tyr Gly Leu Leu
120 125 130
ctt cca gta gaa cct tcc gca agg ttg atc aca caa acg gag ctt tat 547
Leu Pro Val Glu Pro Ser Ala Arg Leu Ile Thr Gln Thr Glu Leu Tyr
135 140 145
cac atc gct cgc aac gtg gtg aac aac tac gac ggt gag ctc acc gcc 595
His Ile Ala Arg Asn Val Val Asn Asn Tyr Asp Gly Glu Leu Thr Ala
150 155 160 165
acc caa acg ccg gcc act gtc acg gaa tac ctc ctc aag ctg gta tcc 643
Thr Gln Thr Pro Ala Thr Val Thr Glu Tyr Leu Leu Lys Leu Val Ser
170 175 180
gaa atg gac aac cac atg gtt acc gca gag gat atc cgt gag gag tcc 691
Glu Met Asp Asn His Met Val Thr Ala Glu Asp Ile Arg Glu Glu Ser
185 190 195
gat ccg ttc att aag ctt ttc gac gaa ctc ccc aag ggc aag ggt cag 739
Asp Pro Phe Ile Lys Leu Phe Asp Glu Leu Pro Lys Gly Lys Gly Gln
200 205 210
cgc gac aac ctc aac gcc gaa atg acg aag tgg cgg gat aca cag gtc 787
Arg Asp Asn Leu Asn Ala Glu Met Thr Lys Trp Arg Asp Thr Gln Val
215 220 225
gct cgc ctc cag tac ttg ccc ctc gtg aag gct ttg aaa gag gaa ctg 835
Ala Arg Leu Gln Tyr Leu Pro Leu Val Lys Ala Leu Lys Glu Glu Leu
230 235 240 245
cac aat cag gct gtg gtg acg ttt ggt gag cag atg tct aag gca gct 883
His Asn Gln Ala Val Val Thr Phe Gly Glu Gln Met Ser Lys Ala Ala
250 255 260
cat ctc gca tcg acg cat cct cag gtg ggc tat tcg cag agg cga cgg 931
His Leu Ala Ser Thr His Pro Gln Val Gly Tyr Ser Gln Arg Arg Arg
265 270 275
ttc cgc gtt gtc atg ctt gat gag tat cag gac acc agc cac tcg cag 979
Phe Arg Val Val Met Leu Asp Glu Tyr Gln Asp Thr Ser His Ser Gln
280 285 290
cgt gtg ttg ttg agc agt ctt ttc ggt ggc acc gat cct ggt ctc act 1027
Arg Val Leu Leu Ser Ser Leu Phe Gly Gly Thr Asp Pro Gly Leu Thr
295 300 305
gtt aat gct gtg ggc gat cca atg cag gcg att tat ggt tgg cga ggt 1075
Val Asn Ala Val Gly Asp Pro Met Gln Ala Ile Tyr Gly Trp Arg Gly
310 315 320 325
gcg aca gcg gcg aac ttg gag aat ttc gtt gac gat ttc ccc gtc atc l123
Ala Thr Ala Ala Asn Leu Glu Asn Phe Val Asp Asp Phe Pro Val Ile
330 335 340
cat ctc gat gga aaa acg aga gca ccg aaa aac gag ttg acc aca agt 1171
His Leu Asp Gly Lys Thr Arg Ala Pro Lys Asn Glu Leu Thr Thr Ser
345 350 355
tgg cga aac cca cca gaa gtc ctc acc ctt gcc aac gca gtt tcg cgg 1219
Trp Arg Asn Pro Pro Glu Val Leu Thr Leu Ala Asn Ala Val Ser Arg
360 365 370
gag gtg ctt ggg tcg ccc gat gcg ccg act cgg acg gtg cag ccg ctg 1267
Glu Val Leu Gly Ser Pro Asp Ala Pro Thr Arg Thr Val Gln Pro Leu
375 380 385
cag cct cgg gaa ggc gca ccg acc ggt gaa gtg tcg ctc gga tgg ttt 1315
Gln Pro Arg Glu Gly Ala Pro Thr Gly Glu Val Ser Leu Gly Trp Phe
390 395 400 405
ggt acg gcg gcg cag gaa cgt gag ttt gtg gct gat gag atg gtg aag 1363
Gly Thr Ala Ala Gln Glu Arg Glu Phe Val Ala Asp Glu Met Val Lys
410 415 420
cat tgg aat gcg cgg gag gag aag ggc aca ttt act gct gcg gtg ttg 1411
His Trp Asn Ala Arg Glu Glu Lys Gly Thr Phe Thr Ala Ala Val Leu
425 430 435
gtg cgt aag aag cgt cat tcg gca ccg atg gcg gag gag ctg acc aag 1459
Val Arg Lys Lys Arg His Ser Ala Pro Met Ala Glu Glu Leu Thr Lys
440 445 450
cgt ggc atc ccc gtg gag atc gtc gga ctg tct ggt ttg ttg gat atc 1507
Arg Gly Ile Pro Val Glu Ile Val Gly Leu Ser Gly Leu Leu Asp Ile
455 460 465
cct gag att gcc gat cta att tct cta gca act atg ctc gtg cgt cct 1555
Pro Glu Ile Ala Asp Leu Ile Ser Leu Ala Thr Met Leu Val Arg Pro
470 475 480 485
cac gat aat cgg gct gcg ttg cgt att ttg gcg ggc ccg cat gtg ggt 1603
His Asp Asn Arg Ala Ala Leu Arg Ile Leu Ala Gly Pro His Val Gly
490 495 500
ttg ggt gtg gcg gat ttg cag cgg ttg cag ggt cgt gcg cgc aat att 1651
Leu Gly Val Ala Asp Leu Gln Arg Leu Gln Gly Arg Ala Arg Asn Ile
505 510 515
gcg ggt cgt gtg tct cgt gag cgt cga gag aag aac cca gat cca tta 1699
Ala Gly Arg Val Ser Arg Glu Arg Arg Glu Lys Asn Pro Asp Pro Leu
520 525 530
gcg gag ctt gat gcg att att gag gaa gcg act gcg att gaa ccg gaa 1747
Ala Glu Leu Asp Ala Ile Ile Glu Glu Ala Thr Ala Ile Glu Pro Glu
535 540 545
gct gtg gtt ggt cta gct gat gcg gtt gcc gat ttg ggt gag ggc gat 1795
Ala Val Val Gly Leu Ala Asp Ala Val Ala Asp Leu Gly Glu Gly Asp
550 555 560 565
cgt ttc agc gag gag ggg ttg agt cgt ttg aag cga ctg gcc act cag 1843
Arg Phe Ser Glu Glu Gly Leu Ser Arg Leu Lys Arg Leu Ala Thr Gln
570 575 580
ttg cga tat ttg cgt aag tac agc ttg ggt cgt tcg gtg gcg gac att 1891
Leu Arg Tyr Leu Arg Lys Tyr Ser Leu Gly Arg Ser Val Ala Asp Ile
585 590 595
ttt gct gat att gaa act gtg ttc aat att cgc acg gag gtg ttg tcg 1939
Phe Ala Asp Ile Glu Thr Val Phe Asn Ile Arg Thr Glu Val Leu Ser
600 605 610
agg cag gat cct cac gcc gat ggt gct gct gga acg gtg cat ttg gat 1987
Arg Gln Asp Pro His Ala Asp Gly Ala Ala Gly Thr Val His Leu Asp
615 620 625
aag ttc gca gaa gag gtc gca agt cac ggt ggc att ggg ctg cct gag 2035
Lys Phe Ala Glu Glu Val Ala Ser His Gly Gly Ile Gly Leu Pro Glu
630 635 640 645
ctg ttg gac tat ttt gag ctc gcg aag gat cag gaa gag ggc ctt gag 2083
Leu Leu Asp Tyr Phe Glu Leu Ala Lys Asp Gln Glu Glu Gly Leu Glu
650 655 660
ccg ggt gag gtg acg gtt cgc agc gat cgt gtg cag atc ctt act gtc 2131
Pro Gly Glu Val Thr Val Arg Ser Asp Arg Val Gln Ile Leu Thr Val
665 670 675
cac aag gct aag ggt ttg gag tgg gat atc gtt tcg gtg ttg cat gcc 2179
His Lys Ala Lys Gly Leu Glu Trp Asp Ile Val Ser Val Leu His Ala
680 685 690
gat gcg tcg acc tat gat gcg aag gct tcg acg tgg ttg aaa aat gtc 2227
Asp Ala Ser Thr Tyr Asp Ala Lys Ala Ser Thr Trp Leu Lys Asn Val
695 700 705
acg atg atc ccg tcg tcg ctt cgt ggc gat gcc ggg acc ggc gcc cca 2275
Thr Met Ile Pro Ser Ser Leu Arg Gly Asp Ala Gly Thr Gly Ala Pro
710 715 720 725
gag atg gat acc tct gag gcg gac gat cgc aaa gct ctg gaa gat tcc 2323
Glu Met Asp Thr Ser Glu Ala Asp Asp Arg Lys Ala Leu Glu Asp Ser
730 735 740
ggc aag gag tac acc agt gaa gtg cgt gag ggg tta cgg gag gaa aat 2371
Gly Lys Glu Tyr Thr Ser Glu Val Arg Glu Gly Leu Arg Glu Glu Asn
745 750 755
tcg cga ctg ttt tat gtc ggt atc acg cgc agc gaa cgg gtg ttg ctc 2419
Ser Arg Leu Phe Tyr Val Gly Ile Thr Arg Ser Glu Arg Val Leu Leu
760 765 770
gtg acg ggt tcg gcg ctt gat gaa agt ggc aca aag gcg aaa gtt ccc 2467
Val Thr Gly Ser Ala Leu Asp Glu Ser Gly Thr Lys Ala Lys Val Pro
775 780 785
tac ggc cac ttg gag atc ctt cgg gat aaa gcg ccg gag tgc gtg gtg 2515
Tyr Gly His Leu Glu Ile Leu Arg Asp Lys Ala Pro Glu Cys Val Val
790 795 800 805
tcg tgg tgg gaa ggg gaa gag ggg gac gtc gaa aag caa aaa cct gcg 2563
Ser Trp Trp Glu Gly Glu Glu Gly Asp Val Glu Lys Gln Lys Pro Ala
810 815 820
gaa ggc gtc ttc ccg caa ctg ctc gcc gct gac tcc tcc ggc gcg gat 2611
Glu Gly Val Phe Pro Gln Leu Leu Ala Ala Asp Ser Ser Gly Ala Asp
825 830 835
ttg gtg cgc ggc ccg cgc gcc gag cca aac aac gag ggc ggg ctg gaa 2659
Lau Val Arg Gly Pro Arg Ala Glu Pro Asn Asn Glu Gly Gly Leu Glu
840 845 850
agc ttg tgg gaa aaa gag gta agc gcg ctt atc gac gaa cac cgc cgc 2707
Ser Leu Trp Glu Lys Glu Val Ser Ala Leu Ile Asp Glu His Arg Arg
855 860 865
ctt tcc aac ccc atc gtc gaa gtc gaa acg ccc cgc gaa ctc acg gcc 2755
Leu Ser Asn Pro Ile Val Glu Val Glu Thr Pro Arg Glu Leu Thr Ala
870 875 880 885
acc gac ctc gtc tct atg aaa aac aat ccc gaa cag ttc gcc cga cga 2803
Thr Asp Leu Val Ser Met Lys Asn Asn Pro Glu Gln Phe Ala Arg Arg
890 895 900
atg cgt cgc cct gtc ccg ttc aaa cca aac acc tac gca aaa cgc ggc 2851
Met Arg Arg Pro Val Pro Phe Lys Pro Asn Thr Tyr Ala Lys Arg Gly
905 910 915
aca ctg ttc cac caa tgg ctc gaa gat cgg ttt ggc agc acc gcg ctt 2899
Thr Leu Phe His Gln Trp Leu Glu Asp Arg Phe Gly Ser Thr Ala Leu
920 925 930
ctc gac gaa acc gag ctc ccc ggc atc gac gag gac tac tcg gat gac 2947
Leu Asp Glu Thr Glu Leu Pro Gly Ile Asp Glu Asp Tyr Ser Asp Asp
935 940 945
gca ttc atc gaa ctc cgc gac gcg ttc cta gga tct act tgg gaa aac 2995
Ala Phe Ile Glu Leu Arg Asp Ala phe Leu Gly Ser Thr Trp Glu Asn
950 955 960 965
cgc aca ccg gaa ttc gtc gaa cat ccc ttc gaa gta acg atc ggc gaa 3043
Arg Thr Pro Glu Phe Val Glu His Pro Phe Glu Val Thr Ile Gly Glu
970 975 980
cac gtc atc cgc ggc cgc atg gac gcc gtc ttc cac acc gat ggc acc 3091
His Val Ile Arg Gly Arg Met Asp Ala Val Phe His Thr Asp Gly Thr
985 990 995
tgg atg gtg gtc gac tgg aaa acc gga cgc acc cca acc ggc ccc gaa 3139
Trp Met Val Val Asp Trp Lys Thr Gly Arg Thr Pro Thr Gly Pro Glu
1000 1005 1010
atg gac gca gcg atc atc cag ctc gct gtc tat aga ctc gcc tgg gca 3187
Met Asp Ala Ala Ile Ile Gln Leu Ala Val Tyr Arg Leu Ala Trp Ala
1015 1020 1025
cga ctc aaa ggc ctc gaa cct gaa gaa gtc cgc gca gca ttc cat tac 3235
Arg Leu Lys Gly Leu Glu Pro Glu Glu Val Arg Ala Ala Phe His Tyr
1030 1035 1040 1045
gtc gcc cac gat cac acc ttt gag ccg aac gat ctg ccg act caa gaa 3283
Val Ala His Asp His Thr Phe Glu Pro Asn Asp Leu Pro Thr Gln Glu
1050 1055 1060
gaa cta gcc cgg ctg ctc agt cag gaa tgatgcgcct aagatcaaac 3330
Glu Leu Ala Arg Leu Leu Ser Gln Glu
1065 1070
aagaatgaaa 3340
<210>42
<211>1070
<212>PRT
<213>谷氨酸棒状杆菌
<400>42
Met Arg Arg Ile Ser Pro Arg Leu Leu Ser Gln Tyr Leu Gly Gln Glu
1 5 10 15
His Ala Pro Thr Asp Gln Gln Ser Ala Ile Ile Gly Ser Glu Pro Gly
20 25 30
Pro Leu Leu Val Val Ala Gly Ala Gly Ala Gly Lys Thr Glu Thr Met
35 40 45
Ala Ala Arg Val Val Trp Leu Val Ala Asn Gly Phe Val Ala Pro Asp
50 55 60
Gln Val Leu Gly Leu Thr Phe Thr Arg Lys Ala Ala Gln Gln Leu Ser
65 70 75 80
Gln Arg Ile Arg Lys Arg Leu Glu Thr Leu Ala Gly Val Pro Arg Leu
85 90 95
Lys Asp Ile Asp Pro Thr Gly Gln Leu Glu Lys Asn Leu Gln Ala Ile
100 105 110
Thr Pro Thr Val Ser Thr Tyr Asp Ser Tyr Ala Gly Thr Leu Ile Arg
115 120 125
Glu Tyr Gly Leu Leu Leu Pro Val Glu Pro Ser Ala Arg Leu Ile Thr
130 135 140
Gln Thr Glu Leu Tyr His Ile Ala Arg Asn Val Val Asn Asn Tyr Asp
145 150 155 160
Gly Glu Leu Thr Ala Thr Gln Thr Pro Ala Thr Val Thr Glu Tyr Leu
165 170 175
Leu Lys Leu Val Ser Glu Met Asp Asn His Met Val Thr Ala Glu Asp
180 185 190
Ile Arg Glu Glu Ser Asp Pro Phe Ile Lys Leu Phe Asp Glu Leu Pro
195 200 205
Lys Gly Lys Gly Gln Arg Asp Asn Leu Asn Ala Glu Met Thr Lys Trp
210 215 220
Arg Asp Thr Gln Val Ala Arg Leu Gln Tyr Leu Pro Leu Val Lys Ala
225 230 235 240
Leu Lys Glu Glu Leu His Asn Gln Ala Val Val Thr Phe Gly Glu Gln
245 250 255
Met Ser Lys Ala Ala His Leu Ala Ser Thr His Pro Gln Val Gly Tyr
260 265 270
Ser Gln Arg Arg Arg Phe Arg Val Val Met Leu Asp Glu Tyr Gln Asp
275 280 285
Thr Ser His Ser Gln Arg Val Leu Leu Ser Ser Leu Phe Gly Gly Thr
290 295 300
Asp Pro Gly Leu Thr Val Asn Ala Val Gly Asp Pro Met Gln Ala Ile
305 310 315 320
Tyr Gly Trp Arg Gly Ala Thr Ala Ala Asn Leu Glu Asn Phe Val Asp
325 330 335
Asp Phe Pro Val Ile His Leu Asp Gly Lys Thr Arg Ala Pro Lys Asn
340 345 350
Glu Leu Thr Thr Ser Trp Arg Asn Pro Pro Glu Val Leu Thr Leu Ala
355 360 365
Asn Ala Val Ser Arg Glu Val Leu Gly Ser Pro Asp Ala Pro Thr Arg
370 375 380
Thr Val Gln Pro Leu Gln Pro Arg Glu Gly Ala Pro Thr Gly Glu Val
385 390 395 400
Ser Leu Gly Trp Phe Gly Thr Ala Ala Gln Glu Arg Glu Phe Val Ala
405 410 415
Asp Glu Met Val Lys His Trp Asn Ala Arg Glu Glu Lys Gly Thr Phe
420 425 430
Thr Ala Ala Val Leu Val Arg Lys Lys Arg His Ser Ala Pro Met Ala
435 440 445
Glu Glu Leu Thr Lys Arg Gly Ile Pro Val Glu Ile Val Gly Leu Ser
450 455 460
Gly Leu Leu Asp Ile Pro Glu Ile Ala Asp Leu Ile Ser Leu Ala Thr
465 470 475 480
Met Leu Val Arg Pro His Asp Asn Arg Ala Ala Leu Arg Ile Leu Ala
485 490 495
Gly Pro His Val Gly Leu Gly Val Ala Asp Leu Gln Arg Leu Gln Gly
500 505 510
Arg Ala Arg Asn Ile Ala Gly Arg Val Ser Arg Glu Arg Arg Glu Lys
515 520 525
Asn Pro Asp Pro Leu Ala Glu Leu Asp Ala Ile Ile Glu Glu Ala Thr
530 535 540
Ala Ile Glu Pro Glu Ala Val Val Gly Leu Ala Asp Ala Val Ala Asp
545 550 555 560
Leu Gly Glu Gly Asp Arg Phe Ser Glu Glu Gly Leu Ser Arg Leu Lys
565 570 575
Arg Leu Ala Thr Gln Leu Arg Tyr Leu Arg Lys Tyr Ser Leu Gly Arg
580 585 590
Ser Val Ala Asp Ile Phe Ala Asp Ile Glu Thr Val Phe Asn Ile Arg
595 600 605
Thr Glu Val Leu Ser Arg Gln Asp Pro His Ala Asp Gly Ala Ala Gly
610 615 620
Thr Val His Leu Asp Lys Phe Ala Glu Glu Val Ala Ser His Gly Gly
625 630 635 640
Ile Gly Leu Pro Glu Leu Leu Asp Tyr Phe Glu Leu Ala Lys Asp Gln
645 650 655
Glu Glu Gly Leu Glu Pro Gly Glu Val Thr Val Arg Ser Asp Arg Val
660 665 670
Gln Ile Leu Thr Val His Lys Ala Lys Gly Leu Glu Trp Asp Ile Val
675 680 685
Ser Val Leu His Ala Asp Ala Ser Thr Tyr Asp Ala Lys Ala Ser Thr
690 695 700
Trp Leu Lys Asn Val Thr Met Ile Pro Ser Ser Leu Arg Gly Asp Ala
705 710 715 720
Gly Thr Gly Ala Pro Glu Met Asp Thr Ser Glu Ala Asp Asp Arg Lys
725 730 735
Ala Leu Glu Asp Ser Gly Lys Glu Tyr Thr Ser Glu Val Arg Glu Gly
740 745 750
Leu Arg Glu Glu Asn Ser Arg Leu Phe Tyr Val Gly Ile Thr Arg Ser
755 760 765
Glu Arg Val Leu Leu Val Thr Gly Ser Ala Leu Asp Glu Ser Gly Thr
770 775 780
Lys Ala Lys Val Pro Tyr Gly His Leu Glu Ile Leu Arg Asp Lys Ala
785 790 795 800
Pro Glu Cys Val Val Ser Trp Trp Glu Gly Glu Glu Gly Asp Val Glu
805 810 815
Lys Gln Lys Pro Ala Glu Gly Val Phe Pro Gln Leu Leu Ala Ala Asp
820 825 830
Ser Ser Gly Ala Asp Leu Val Arg Gly Pro Arg Ala Glu Pro Asn Asn
835 840 845
Glu Gly Gly Leu Glu Ser Leu Trp Glu Lys Glu Val Ser Ala Leu Ile
850 855 860
Asp Glu His Arg Arg Leu Ser Asn Pro Ile Val Glu Val Glu Thr Pro
865 870 875 880
Arg Glu Leu Thr Ala Thr Asp Leu Val Ser Met Lys Asn Asn Pro Glu
885 890 895
Gln Phe Ala Arg Arg Met Arg Arg Pro Val Pro Phe Lys Pro Asn Thr
900 905 910
Tyr Ala Lys Arg Gly Thr Leu Phe His Gln Trp Leu Glu Asp Arg Phe
915 920 925
Gly Ser Thr Ala Leu Leu Asp Glu Thr Glu Leu Pro Gly Ile Asp Glu
930 935 940
Asp Tyr Ser Asp Asp Ala Phe Ile Glu Leu Arg Asp Ala Phe Leu Gly
945 950 955 960
Ser Thr Trp Glu Asn Arg Thr Pro Glu Phe Val Glu His Pro Phe Glu
965 970 975
Val Thr Ile Gly Glu His Val Ile Arg Gly Arg Met Asp Ala Val Phe
980 985 990
His Thr Asp Gly Thr Trp Met Val Val Asp Trp Lys Thr Gly Arg Thr
995 1000 1005
Pro Thr Gly Pro Glu Met Asp Ala Ala Ile Ile Gln Leu Ala Val Tyr
1010 1015 1020
Arg Leu Ala Trp Ala Arg Leu Lys Gly Leu Glu Pro Glu Glu Val Arg
1025 1030 1035 1040
Ala Ala Phe His Tyr Val Ala His Asp His Thr Phe Glu Pro Asn Asp
1045 1050 1055
Leu Pro Thr Gln Glu Glu Leu Ala Arg Leu Leu Ser Gln Glu
1060 1065 1070
<210>43
<211>3895
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3865)
<223>RXA01770
<400>43
tgctcatgac aggtaattca acgcgttacc ctgctgcagc ataaaagaac ctgcctcaac 60
acacctccac tttttctact tttaagaaag ccacattctc atg cca att atc att 115
Met Pro Ile Ile Ile
1 5
gat aac ctc aat tct gac gac gac tcc acc atc ggc act gcc acc gaa 163
Asp Asn Leu Asn Ser Asp Asp Asp Ser Thr Ile Gly Thr Ala Thr Glu
10 15 20
tac aac cct gac act gac gcc gat tta ctt gat gct att aac gct gat 211
Tyr Asn Pro Asp Thr Asp Ala Asp Leu Leu Asp Ala Ile Asn Ala Asp
25 30 35
gct gac ctt gat ggc gac gcc acc atc agc act aac gcg aca gaa gaa 259
Ala Asp Leu Asp Gly Asp Ala Thr Ile Ser Thr Asn Ala Thr Glu Glu
40 45 50
ggt gta gac gca gca gct gaa aaa cct aag aaa aag cgt aaa gcc cct 307
Gly Val Asp Ala Ala Ala Glu Lys Pro Lys Lys Lys Arg Lys Ala Pro
55 60 65
gct ctg aag cct aaa gga ctc acg gca aag ttc ttc cac cgt gat ctt 355
Ala Leu Lys Pro Lys Gly Leu Thr Ala Lys Phe Phe His Arg Asp Leu
70 75 80 85
act ggc gta ggt ggt agg acc ggt cgc ctc aac aag aac gta cac ccg 403
Thr Gly Val Gly Gly Arg Thr Gly Arg Leu Asn Lys Asn Val His Pro
90 95 100
acc aac cca gat ctg tcc tac cag cca gtc tct gat gtc tac acc cca 451
Thr Asn Pro Asp Leu Ser Tyr Gln Pro Val Ser Asp Val Tyr Thr Pro
105 110 115
caa tca gca gat cac aag ggt att aag acc cgc tac atc ctt acc cat 499
Gln Ser Ala Asp His Lys Gly Ile Lys Thr Arg Tyr Ile Leu Thr His
120 125 130
cca acc ccc gct gtt gtt ctc agt gag tcc atc agc aac gcg ttt cat 547
Pro Thr Pro Ala Val Val Leu Ser Glu Ser Ile Ser Asn Ala Phe His
135 140 145
gtc tct acc ctg cgt cgc aac aat aat gtc aac aac tct gat tcg gaa 595
Val Ser Thr Leu Arg Arg Asn Asn Asn Val Asn Asn Ser Asp Ser Glu
150 155 160 165
ttg gct gcc tgg ccg tac ctc tac caa ctc gat att ccg cag ctg gac 643
Leu Ala Ala Trp Pro Tyr Leu Tyr Gln Leu Asp Ile Pro Gln Leu Asp
170 175 180
cag atg att aat gtc gct gac atc tgt gat tac cat ttc cac gga tat 691
Gln Met Ile Asn Val Ala Asp Ile Cys Asp Tyr His Phe His Gly Tyr
185 190 195
aac ctg tgg gtg gat ttt acc ccg cag act atc gct cta cga tcc ggt 739
Asn Leu Trp Val Asp Phe Thr Pro Gln Thr Ile Ala Leu Arg Ser Gly
200 205 210
aag acg gta ctc gat gac ggt acc acc gcc tct gat aac acc act cat 787
Lys Thr Val Leu Asp Asp Gly Thr Thr Ala Ser Asp Asn Thr Thr His
215 220 225
gtc tat tac cgc gtc acc gtt cac gtt att gcc ggt caa gat cat gga 835
Val Tyr Tyr Arg Val Thr Val His Val Ile Ala Gly Gln Asp His Gly
230 235 240 245
tct act ctg ctt gat gac cag ggc aac cag gtg ctt gat agg gat gat 883
Ser Thr Leu Leu Asp Asp Gln Gly Asn Gln Val Leu Asp Arg Asp Asp
250 255 260
aat cct att tct acc cca agt atc aag cgt att ggc gct gtc act gat 931
Asn Pro Ile Ser Thr Pro Ser Ile Lys Arg Ile Gly Ala Val Thr Asp
265 270 275
ctt ttc gat cac aat cct ttt ggc ttc gct agt gta aac tct ttc gca 979
Leu Phe Asp His Asn Pro Phe Gly Phe Ala Ser Val Asn Ser Phe Ala
280 285 290
ttt gtc gat ttc tca tgg gac cca gcc acc acc ttg gtc gat atg ctc 1027
Phe Val Asp Phe Ser Trp Asp Pro Ala Thr Thr Leu Val Asp Met Leu
295 300 305
aac aac ctt gat tca tat ctc tct aat cac atc aat att gcc agc tcc 1075
Asn Asn Leu Asp Ser Tyr Leu Ser Asn His Ile Asn Ile Ala Ser Ser
310 315 320 325
cca acc ccc att gcc ctc gat atg gtt gtg ctc aat gaa tgg tct gaa 1123
Pro Thr Pro Ile Ala Leu Asp Met Val Val Leu Asn Glu Trp Ser Glu
330 335 340
aag tcc tat cag ctg tgc gaa cgc gtt gtt gca cag gca aag ctc atc 1171
Lys Ser Tyr Gln Leu Cys Glu Arg Val Val Ala Gln Ala Lys Leu Ile
345 350 355
aac agc aac aag atc acc gca cat gtg agt gat gtc atc aag caa aat 1219
Asn Ser Asn Lys Ile Thr Ala His Val Ser Asp Val Ile Lys Gln Asn
360 365 370
gcc cac aat atc ttg tgg ttt acc gaa cag atg aac cct ggc acc acc 1267
Ala His Asn Ile Leu Trp Phe Thr Glu Gln Met Asn Pro Gly Thr Thr
375 380 385
aac ctc agc gag gta cct atc tcc aag aag tct atg ctg ccg atg tct 1315
Asn Leu Ser Glu Val Pro Ile Ser Lys Lys Ser Met Leu Pro Met Ser
390 395 400 405
cgc cag cta cgt att ttg gag cac tac gat gta cca ctg acc gcg tac 1363
Arg Gln Leu Arg Ile Leu Glu His Tyr Asp Val Pro Leu Thr Ala Tyr
410 415 420
tct gct ctt ttc tgg act gtc agc gcc att aag aat gaa tct atg gtg 1411
Ser Ala Leu Phe Trp Thr Val Ser Ala Ile Lys Asn Glu Ser Met Val
425 430 435
cag tac ctt gtg cga cag aat atg cag ctc acc ttg agc tca aat ctg 1459
Gln Tyr Leu Val Arg Gln Asn Met Gln Leu Thr Leu Ser Ser Asn Leu
440 445 450
gat gca ctc aac tcc att gtc tcc cag ctc cct gta cca gat aag gac 1507
Asp Ala Leu Asn Ser Ile Val Ser Gln Leu Pro Val Pro Asp Lys Asp
455 460 465
gtt gtg gct gct tct ggc tac cag att cag cca cac ttc tcc acg cag 1555
Val Val Ala Ala Ser Gly Tyr Gln Ile Gln Pro His Phe Ser Thr Gln
470 475 480 485
cag cgt gaa gca atc acc act gat aat cct ctc gcg att att cag gcc 1603
Gln Arg Glu Ala Ile Thr Thr Asp Asn Pro Leu Ala Ile Ile Gln Ala
490 495 500
ggt gct ggt acc ggt aaa tcc acc gtg att ctg gaa cgc att gag tac 1651
Gly Ala Gly Thr Gly Lys Ser Thr Val Ile Leu Glu Arg Ile Glu Tyr
505 510 515
ttg tgc gct gca ggc acc aac cct gaa gag att gct gtg ctg tct ttt 1699
Leu Cys Ala Ala Gly Thr Asn Pro Glu Glu Ile Ala Val Leu Ser Phe
520 525 530
act aat gct gcc gcc gat aac atc aca gca aaa aat gac aag gtg acc 1747
Thr Asn Ala Ala Ala Asp Asn Ile Thr Ala Lys Asn Asp Lys Val Thr
535 540 545
tct atg acc atc tcc aag atg gtt cat gag att tac gca cac aat ttc 1795
Ser Met Thr Ile Ser Lys Met Val His Glu Ile Tyr Ala His Asn Phe
550 555 560 565
cct gat cat gag atc tcc acc atc gac acc att atc aat acg ctt gat 1843
Pro Asp His Glu Ile Ser Thr Ile Asp Thr Ile Ile Asn Thr Leu Asp
570 575 580
att gaa tac ggc gat caa atg gtg acc tcc gat tac atg att cag ctc 1891
Ile Glu Tyr Gly Asp Gln Met Val Thr Ser Asp Tyr Met Ile Gln Leu
585 590 595
cgt gac ctg ctc tat aag gtc atg acg cag ggt ggc aac gcc aac ctg 1939
Arg Asp Leu Leu Tyr Lys Val Met Thr Gln Gly Gly Asn Ala Asn Leu
600 605 610
aca gcg ttg agc atc ttc atg gaa tcc cat atc gag gcg ttc atc tcg 1987
Thr Ala Leu Ser Ile Phe Met Glu Ser His Ile Glu Ala Phe Ile Ser
615 620 625
gtg ttg gat cag atc aag cag act tct ctt gag ctg gaa atc atc atc 2035
Val Leu Asp Gln Ile Lys Gln Thr Ser Leu Glu Leu Glu Ile Ile Ile
630 635 640 645
tgc tac ctg ctc ttg gat aag ctg atc gag cct cat gca tcg ccg aag 2083
Cys Tyr Leu Leu Leu Asp Lys Leu Ile Glu Pro His Ala Ser Pro Lys
650 655 660
tac ctc att att gat gag gtg cag gac aac tcg gtc ttc gag ttc gtc 2131
Tyr Leu Ile Ile Asp Glu Val Gln Asp Asn Ser Val Phe Glu Phe Val
665 670 675
ttt gca ctt cgt ttt gcg gca aag cac aat acg agt ctg tac ttg gtg 2179
Phe Ala Leu Arg Phe Ala Ala Lys His Asn Thr Ser Leu Tyr Leu Val
680 685 690
ggt gac tcg tca cag acc ttg tat gag ttc cgt tct gct aac cct aag 2227
Gly Asp Ser Ser Gln Thr Leu Tyr Glu Phe Arg Ser Ala Asn Pro Lys
695 700 705
gct ctg aac tcc ctg gaa gca tct ggt gtt ttc ggt acc tac cgt ttg 2275
Ala Leu Asn Ser Leu Glu Ala Ser Gly Val Phe Gly Thr Tyr Arg Leu
710 715 720 725
acc acc aat tac cgt tcc aac cag gaa att ctt gac ttc gct aat atc 2323
Thr Thr Asn Tyr Arg Ser Asn Gln Glu Ile Leu Asp Phe Ala Asn Ile
730 735 740
cac ctc tcg gat att gaa gct aac cag ttc gca ggt att cag ctc tac 2371
His Leu Ser Asp Ile Glu Ala Asn Gln Phe Ala Gly Ile Gln Leu Tyr
745 750 755
gcc aac tct ttt gat gca cct act gcg gac agc ttc aaa gaa aag gtt 2419
Ala Asn Ser Phe Asp Ala Pro Thr Ala Asp Ser Phe Lys Glu Lys Val
760 765 770
gaa ctg gac atg cac cat gtg tct aag cag tct gag ttt acc gac agc 2467
Glu Leu Asp Met His His Val Ser Lys Gln Ser Glu Phe Thr Asp Ser
775 780 785
att cct tat ttc atg gag tcc aat aaa gct cgc ttt gat gct gcg atc 2515
Ile Pro Tyr Phe Met Glu Ser Asn Lys Ala Arg Phe Asp Ala Ala Ile
790 795 800 805
ttg aac aat gag caa acc att gtg ctt gct cat tct ggt cgt gaa att 2563
Leu Asn Asn Glu Gln Thr Ile Val Leu Ala His Ser Gly Arg Glu Ile
810 815 820
cgt gca gcg cag caa gcg cta gcc gag atg tac ccc agc atc acg gtg 2611
Arg Ala Ala Gln Gln Ala Leu Ala Glu Met Tyr Pro Ser Ile Thr Val
825 830 835
cgt aac ttg cag tcc gat aag ggt ttc aac aac acg gta ttt tcg acc 2659
Arg Asn Leu Gln Ser Asp Lys Gly Phe Asn Asn Thr Val Phe Ser Thr
840 845 850
ttt att aag gat ttc tgg ttt gag gtc acc gct gta gac cca gct cat 2707
Phe Ile Lys Asp Phe Trp Phe Glu Val Thr Ala Val Asp Pro Ala His
855 860 865
gcc gca ttt acc ttt acc agc caa gtc acc gct cat cta gat aag ttg 2755
Ala Ala Phe Thr Phe Thr Ser Gln Val Thr Ala His Leu Asp Lys Leu
870 875 880 885
gta cgc gga aag cgt gag cag atg gag gat cgc gtt atc cgg tct atg 2803
Val Arg Gly Lys Arg Glu Gln Met Glu Asp Arg Val Ile Arg Ser Met
890 895 900
gct gca tgg tgg cgt gaa aat gag cgt gac atc cag ggt tgg gtg cag 2851
Ala Ala Trp Trp Arg Glu Asn Glu Arg Asp Ile Gln Gly Trp Val Gln
905 910 915
caa act cag tcc ggt gcg atc acc aac gaa gag ttc ttc tac cgt ctt 2899
Gln Thr Gln Ser Gly Ala Ile Thr Asn Glu Glu Phe Phe Tyr Arg Leu
920 925 930
cgt cag tgc att ctt gac tat gaa atc agg aat aac cga gca cgt cag 2947
Arg Gln Cys Ile Leu Asp Tyr Glu Ile Arg Asn Asn Arg Ala Arg Gln
935 940 945
tcc atg ctc aat gcg cgc aac aac gct aac aag gag gct gtc gca cag 2995
Ser Met Leu Asn Ala Arg Asn Asn Ala Asn Lys Glu Ala Val Ala Gln
950 955 960 965
gag aaa cca ctg ctc atg gtc tcc acc att cac agt gca aag ggt ctg 3043
Glu Lys Pro Leu Leu Met Val Ser Thr Ile His Ser Ala Lys Gly Leu
970 975 980
gaa ttc gat aat gtc atc gtg ctt caa aaa cca agc tcc gat gca gag 3091
Glu Phe Asp Asn Val Ile Val Leu Gln Lys Pro Ser Ser Asp Ala Glu
985 990 995
atg acc gaa gaa ggt aag cgc gca acc tat gtg gcg ctg acc cgt gca 3139
Met Thr Glu Glu Gly Lys Arg Ala Thr Tyr Val Ala Leu Thr Arg Ala
1000 1005 1010
aaa aag cgt gaa ctc att att gct ggt tct act cgc gca tac cca cgc 3187
Lys Lys Arg Glu Leu Ile Ile Ala Gly Ser Thr Arg Ala Tyr Pro Arg
1015 1020 1025
atc gtc acc gat tat gag caa atc gtt gat ctt ctt gaa aag cgc gat 3235
Ile Val Thr Asp Tyr Glu Gln Ile Val Asp Leu Leu Glu Lys Arg Asp
1030 1035 1040 1045
gag gag cgc aaa att aag gag gaa gag gct gca gca ctc gcc acc ctt 3283
Glu Glu Arg Lys Ile Lys Glu Glu Glu Ala Ala Ala Leu Ala Thr Leu
1050 1055 1060
gaa gct gaa cag gag acg cgc gcc ctt gct gct gct gag gcg cag gct 3331
Glu Ala Glu Gln Glu Thr Arg Ala Leu Ala Ala Ala Glu Ala Gln Ala
1065 1070 1075
cag gcg ttg ttg ctt gag cac aac ccg tgg ttg cgt gat ctc agc gat 3379
Gln Ala Leu Leu Leu Glu His Asn Pro Trp Leu Arg Asp Leu Ser Asp
1080 1085 1090
gaa gaa gtc act gct cta act gag caa gag atc atc aac aat gtt gag 3427
Glu Glu Val Thr Ala Leu Thr Glu Gln Glu Ile Ile Asn Asn Val Glu
1095 1100 1105
gca gcc cta cag att gaa gaa gag gag gaa gag gct cgt gca ctc gct 3475
Ala Ala Leu Gln Ile Glu Glu Glu Glu Glu Glu Ala Arg Ala Leu Ala
1110 1115 1120 1125
gca gca gag cca gca att cag cag tac ttg tct cag ttt gct ttc gat 3523
Ala Ala Glu Pro Ala Ile Gln Gln Tyr Leu Ser Gln Phe Ala Phe Asp
1130 1135 1140
gag ttc ccg gac gac gac aat gta gcc aac act gtc gta cat gta gca 3571
Glu Phe Pro Asp Asp Asp Asn Val Ala Asn Thr Val Val His Val Ala
1145 1150 1155
cca cag cct att ctc cac cag gca gtg cct gca gat gtg act gta caa 3619
Pro Gln Pro Ile Leu His Gln Ala Val Pro Ala Asp Val Thr Val Gln
1160 1165 1170
tca agc acc gcc ccc gta aca cct gtt gtt gcg gat ctt gag gta acc 3667
Ser Ser Thr Ala Pro Val Thr Pro Val Val Ala Asp Leu Glu Val Thr
1175 1180 1185
act gtt gca gct gat cca gtc gaa ccg act atc gtt gct gca cag cct 3715
Thr Val Ala Ala Asp Pro Val Glu Pro Thr Ile Val Ala Ala Gln Pro
1190 1195 1200 1205
gag gtg gac gac aac ctt gtc tac agc acg tcc acc cct aat agt cac 3763
Glu Val Asp Asp Asn Leu Val Tyr Ser Thr Ser Thr Pro Asn Ser His
1210 1215 1220
agt gac gtt att gca gtg aac tct gat acc tca gaa aac gct gct gtt 3811
Ser Asp Val Ile Ala Val Asn Ser Asp Thr Ser Glu Asn Ala Ala Val
1225 1230 1235
aat cca gtt ctg tcc gat att gag gca ctc cga gca att ttc aac aac 3859
Asn Pro Val Leu Ser Asp Ile Glu Ala Leu Arg Ala Ile Phe Asn Asn
1240 1245 1250
cag gac taaaacacct gctgctgttg agcgcttctc 3895
Gln Asp
1255
<210>44
<211>1255
<212>PRT
<213>谷氨酸棒状杆菌
<400>44
Met Pro Ile Ile Ile Asp Asn Leu Asn Ser Asp Asp Asp Ser Thr Ile
1 5 10 15
Gly Thr Ala Thr Glu Tyr Asn Pro Asp Thr Asp Ala Asp Leu Leu Asp
20 25 30
Ala Ile Asn Ala Asp Ala Asp Leu Asp Gly Asp Ala Thr Ile Ser Thr
35 40 45
Asn Ala Thr Glu Glu Gly Val Asp Ala Ala Ala Glu Lys Pro Lys Lys
50 55 60
Lys Arg Lys Ala Pro Ala Leu Lys Pro Lys Gly Leu Thr Ala Lys Phe
65 70 75 80
Phe His Arg Asp Leu Thr Gly Val Gly Gly Arg Thr Gly Arg Leu Asn
85 90 95
Lys Asn Val His Pro Thr Asn Pro Asp Leu Ser Tyr Gln Pro Val Ser
100 105 110
Asp Val Tyr Thr Pro Gln Ser Ala Asp His Lys Gly Ile Lys Thr Arg
115 120 125
Tyr Ile Leu Thr His Pro Thr Pro Ala Val Val Leu Ser Glu Ser Ile
130 135 140
Ser Asn Ala Phe His Val Ser Thr Leu Arg Arg Asn Asn Asn Val Asn
145 150 155 160
Asn Ser Asp Ser Glu Leu Ala Ala Trp Pro Tyr Leu Tyr Gln Leu Asp
165 170 175
Ile Pro Gln Leu Asp Gln Met Ile Asn Val Ala Asp Ile Cys Asp Tyr
180 185 190
His Phe His Gly Tyr Asn Leu Trp Val Asp Phe Thr Pro Gln Thr Ile
195 200 205
Ala Leu Arg Ser Gly Lys Thr Val Leu Asp Asp Gly Thr Thr Ala Ser
210 215 220
Asp Asn Thr Thr His Val Tyr Tyr Arg Val Thr Val His Val Ile Ala
225 230 235 240
Gly Gln Asp His Gly Ser Thr Leu Leu Asp Asp Gln Gly Asn Gln Val
245 250 255
Leu Asp Arg Asp Asp Asn Pro Ile Ser Thr Pro Ser Ile Lys Arg Ile
260 265 270
Gly Ala Val Thr Asp Leu Phe Asp His Asn Pro Phe Gly Phe Ala Ser
275 280 285
Val Asn Ser Phe Ala Phe Val Asp Phe Ser Trp Asp Pro Ala Thr Thr
290 295 300
Leu Val Asp Met Leu Asn Asn Leu Asp Ser Tyr Leu Ser Asn His Ile
305 310 315 320
Asn Ile Ala Ser Ser Pro Thr Pro Ile Ala Leu Asp Met Val Val Leu
325 330 335
Asn Glu Trp Ser Glu Lys Ser Tyr Gln Leu Cys Glu Arg Val Val Ala
340 345 350
Gln Ala Lys Leu Ile Asn Ser Asn Lys Ile Thr Ala His Val Ser Asp
355 360 365
Val Ile Lys Gln Asn Ala His Asn Ile Leu Trp Phe Thr Glu Gln Met
370 375 380
Asn Pro Gly Thr Thr Asn Leu Ser Glu Val Pro Ile Ser Lys Lys Ser
385 390 395 400
Met Leu Pro Met Ser Arg Gln Leu Arg Ile Leu Glu His Tyr Asp Val
405 410 415
Pro Leu Thr Ala Tyr Ser Ala Leu Phe Trp Thr Val Ser Ala Ile Lys
420 425 430
Asn Glu Ser Met Val Gln Tyr Leu Val Arg Gln Asn Met Gln Leu Thr
435 440 445
Leu Ser Ser Asn Leu Asp Ala Leu Asn Ser Ile Val Ser Gln Leu Pro
450 455 460
Val Pro Asp Lys Asp Val Val Ala Ala Ser Gly Tyr Gln Ile Gln Pro
465 470 475 480
His Phe Ser Thr Gln Gln Arg Glu Ala Ile Thr Thr Asp Asn Pro Leu
485 490 495
Ala Ile Ile Gln Ala Gly Ala Gly Thr Gly Lys Ser Thr Val Ile Leu
500 505 510
Glu Arg Ile Glu Tyr Leu Cys Ala Ala Gly Thr Asn Pro Glu Glu Ile
515 520 525
Ala Val Leu Ser Phe Thr Asn Ala Ala Ala Asp Asn Ile Thr Ala Lys
530 535 540
Asn Asp Lys Val Thr Ser Met Thr Ile Ser Lys Met Val His Glu Ile
545 550 555 560
Tyr Ala His Asn Phe Pro Asp His Glu Ile Ser Thr Ile Asp Thr Ile
565 570 575
Ile Asn Thr Leu Asp Ile Glu Tyr Gly Asp Gln Met Val Thr Ser Asp
580 585 590
Tyr Met Ile Gln Leu Arg Asp Leu Leu Tyr Lys Val Met Thr Gln Gly
595 600 605
Gly Asn Ala Asn Leu Thr Ala Leu Ser Ile Phe Met Glu Ser His Ile
610 615 620
Glu Ala Phe Ile Ser Val Leu Asp Gln Ile Lys Gln Thr Ser Leu Glu
625 630 635 640
Leu Glu Ile Ile Ile Cys Tyr Leu Leu Leu Asp Lys Leu Ile Glu Pro
645 650 655
His Ala Ser Pro Lys Tyr Leu Ile Ile Asp Glu Val Gln Asp Asn Ser
660 665 670
Val Phe Glu Phe Val Phe Ala Leu Arg Phe Ala Ala Lys His Asn Thr
675 680 685
Ser Leu Tyr Leu Val Gly Asp Ser Ser Gln Thr Leu Tyr Glu Phe Arg
690 695 700
Ser Ala Asn Pro Lys Ala Leu Asn Ser Leu Glu Ala Ser Gly Val Phe
705 710 715 720
Gly Thr Tyr Arg Leu Thr Thr Asn Tyr Arg Ser Asn Gln Glu Ile Leu
725 730 735
Asp Phe Ala Asn Ile His Leu Ser Asp Ile Glu Ala Asn Gln Phe Ala
740 745 750
Gly Ile Gln Leu Tyr Ala Asn Ser Phe Asp Ala Pro Thr Ala Asp Ser
755 760 765
Phe Lys Glu Lys Val Glu Leu Asp Met His His Val Ser Lys Gln Ser
770 775 780
Glu Phe Thr Asp Ser Ile Pro Tyr Phe Met Glu Ser Asn Lys Ala Arg
785 790 795 800
Phe Asp Ala Ala Ile Leu Asn Asn Glu Gln Thr Ile Val Leu Ala His
805 810 815
Ser Gly Arg Glu Ile Arg Ala Ala Gln Gln Ala Leu Ala Glu Met Tyr
820 825 830
Pro Ser Ile Thr Val Arg Asn Leu Gln Ser Asp Lys Gly Phe Asn Asn
835 840 845
Thr Val Phe Ser Thr Phe Ile Lys Asp Phe Trp Phe Glu Val Thr Ala
850 855 860
Val Asp Pro Ala His Ala Ala Phe Thr Phe Thr Ser Gln Val Thr Ala
865 870 875 880
His Leu Asp Lys Leu Val Arg Gly Lys Arg Glu Gln Met Glu Asp Arg
885 890 895
Val Ile Arg Ser Met Ala Ala Trp Trp Arg Glu Asn Glu Arg Asp Ile
900 905 910
Gln Gly Trp Val Gln Gln Thr Gln Ser Gly Ala Ile Thr Asn Glu Glu
915 920 925
Phe Phe Tyr Arg Leu Arg Gln Cys Ile Leu Asp Tyr Glu Ile Arg Asn
930 935 940
Asn Arg Ala Arg Gln Ser Met Leu Asn Ala Arg Asn Asn Ala Asn Lys
945 950 955 960
Glu Ala Val Ala Gln Glu Lys Pro Leu Leu Met Val Ser Thr Ile His
965 970 975
Ser Ala Lys Gly Leu Glu Phe Asp Asn Val Ile Val Leu Gln Lys Pro
980 985 990
Ser Ser Asp Ala Glu Met Thr Glu Glu Gly Lys Arg Ala Thr Tyr Val
995 1000 1005
Ala Leu Thr Arg Ala Lys Lys Arg Glu Leu Ile Ile Ala Gly Ser Thr
1010 1015 1020
Arg Ala Tyr Pro Arg Ile Val Thr Asp Tyr Glu Gln Ile Val Asp Leu
1025 1030 1035 1040
Leu Glu Lys Arg Asp Glu Glu Arg Lys Ile Lys Glu Glu Glu Ala Ala
1045 1050 1055
Ala Leu Ala Thr Leu Glu Ala Glu Gln Glu Thr Arg Ala Leu Ala Ala
1060 1065 1070
Ala Glu Ala Gln Ala Gln Ala Leu Leu Leu Glu His Asn Pro Trp Leu
1075 1080 1085
Arg Asp Leu Ser Asp Glu Glu Val Thr Ala Leu Thr Glu Gln Glu Ile
1090 1095 1100
Ile Asn Asn Val Glu Ala Ala Leu Gln Ile Glu Glu Glu Glu Glu Glu
1105 1110 1115 1120
Ala Arg Ala Leu Ala Ala Ala Glu Pro Ala Ile Gln Gln Tyr Leu Ser
1125 1130 1135
Gln Phe Ala Phe Asp Glu Phe Pro Asp Asp Asp Asn Val Ala Asn Thr
1140 1145 1150
Val Val His Val Ala Pro Gln Pro Ile Leu His Gln Ala Val Pro Ala
1155 1160 1165
Asp Val Thr Val Gln Ser Ser Thr Ala Pro Val Thr Pro Val Val Ala
1170 1175 1180
Asp Leu Glu Val Thr Thr Val Ala Ala Asp Pro Val Glu Pro Thr Ile
1185 1190 1195 1200
Val Ala Ala Gln Pro Glu Val Asp Asp Asn Leu Val Tyr Ser Thr Ser
1205 1210 1215
Thr Pro Asn Ser His Ser Asp Val Ile Ala Val Asn Ser Asp Thr Ser
1220 1225 1230
Glu Asn Ala Ala Val Asn Pro Val Leu Ser Asp Ile Glu Ala Leu Arg
1235 1240 1245
Ala Ile Phe Asn Asn Gln Asp
1250 1255
<210>45
<211>5068
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(5038)
<223>RXA01772
<400>45
ccagaaggaa tcctcccgca ccggctggct cgttagcctg aagtagcact tcgaatctaa 60
gaccaccaaa tggacatgtc ctttgagtga ataggacaca atg gaa tgc atg tcc 115
Met Glu Cys Met Ser
1 5
acg att act gat gct gta tcc gcc ctg cgc aca ctg cct tcc cag tct 163
Thr Ile Thr Asp Ala Val Ser Ala Leu Arg Thr Leu Pro Ser Gln Ser
10 15 20
gcg cag ggt ttg gct ttt gaa aaa ctc atg gtc aac ttc att aag tct 211
Ala Gln Gly Leu Ala Phe Glu Lys Leu Met Val Asn Phe Ile Lys Ser
25 30 35
gac ccc act ctc tcc act gag ttt gat gaa gtc cat cgt tgg gtc gat 259
Asp Pro Thr Leu Ser Thr Glu Phe Asp Glu Val His Arg Trp Val Asp
40 45 50
tgg cca tat aac ggt ggc acc atg gat act ggc atc gac tta gtg gct 307
Trp Pro Tyr Asn Gly Gly Thr Met Asp Thr Gly Ile Asp Leu Val Ala
55 60 65
tat aac aag gac gat gat gct tat acg gcg atc cag tgc aag ttt tat 355
Tyr Asn Lys Asp Asp Asp Ala Tyr Thr Ala Ile Gln Cys Lys Phe Tyr
70 75 80 85
ctt ccc act act tcc ctg gca aaa gga cag cta gat tcc ttc ttt gaa 403
Leu Pro Thr Thr Ser Leu Ala Lys Gly Gln Leu Asp Ser Phe Phe Glu
90 95 100
gcc tcg gga cgc act ttt gaa acc cct gaa gga aca cgc tcc ttt agc 451
Ala Ser Gly Arg Thr Phe Glu Thr Pro Glu Gly Thr Arg Ser Phe Ser
105 110 115
aat aga ctc gtt att tcc act act gat aag tgg agt tca aat gcg gaa 499
Asn Arg Leu Val Ile Ser Thr Thr Asp Lys Trp Ser Ser Asn Ala Glu
120 125 130
aag atg ttg gag aac caa acc att cca act aac cgc att ggt cta tct 547
Lys Met Leu Glu Asn Gln Thr Ile Pro Thr Asn Arg Ile Gly Leu Ser
135 140 145
gca att gcg gaa tcc ccg att gac tgg gat att gcc tat cca ggt tct 595
Ala Ile Ala Glu Ser Pro Ile Asp Trp Asp Ile Ala Tyr Pro Gly Ser
150 155 160 165
gaa tta act att aac ctg cag ctt aaa gag ccg tat agc cct cgt cct 643
Glu Leu Thr Ile Asn Leu Gln Leu Lys Glu Pro Tyr Ser Pro Arg Pro
170 175 180
cac cag caa act gct att gaa aaa gca att gaa ggc ttc caa act cat 691
His Gln Gln Thr Ala Ile Glu Lys Ala Ile Glu Gly Phe Gln Thr His
185 190 195
gac cgt ggc aeg ctc atc atg gct tgc ggt acc gga aaa acc ttt act 739
Asp Arg Gly Lys Leu Ile Met Ala Cys Gly Thr Gly Lys Thr Phe Thr
200 205 210
gcc ctg cgt ctt tct gaa gag gtt gcg cga ctc aat ggc aat aaa gct 787
Ala Leu Arg Leu Ser Glu Glu Val Ala Arg Leu Asn Gly Asn Lys Ala
215 220 225
cgc att ctt ttt cta gtc cct tcc atc agc ttg ctt tcg caa aca etc 835
Arg Ile Leu Phe Leu Val Pro Ser Ile Ser Leu Leu Ser Gln Thr Leu
230 235 240 245
aaa gag tgg act gca caa aag aca atg gat ctt cgt cct gtc gcc gta 883
Lys Glu Trp Thr Ala Gln Lys Thr Met Asp Leu Arg Pro Val Ala Val
250 255 260
tgt tct gac tcc aag gtc tcc aag gct gca gaa gat att gct gct tat 931
Cys Ser Asp Ser Lys Val Ser Lys Ala Ala Glu Asp Ile Ala Ala Tyr
265 270 275
gat ctt gaa gtt ccg gta agt act gat ggt gca ttg att gcg gaa aaa 979
Asp Leu Glu Val Pro Val Ser Thr Asp Gly Ala Leu Ile Ala Glu Lys
280 285 290
ctg gag cat cgt aag cga gca gca gga ttg acc gtg gtg ttc tct act 1027
Leu Glu His Arg Lys Arg Ala Ala Gly Leu Thr Val Val Phe Ser Thr
295 300 305
tac cag tcc ctt cct gct gtt cac gcg gct caa gaa gct ggg gct gag 1075
Tyr Gln Ser Leu Pro Ala Val His Ala Ala Gln Glu Ala Gly Ala Glu
310 315 320 325
ccc ttt gat ctt gtc atc tgc gat gaa gcc cac cgc acc aca ggc atc 1123
Pro Phe Asp Leu Val Ile Cys Asp Glu Ala His Arg Thr Thr Gly Ile
330 335 340
acc ttg gct gga gaa gac cct tca aac ttc acc cgc att cat gac gct 1171
Thr Leu Ala Gly Glu Asp Pro Ser Asn Phe Thr Arg Ile His Asp Ala
345 350 355
tct tat atc aag gca gca aag cgg ctg tac atg acg gca acc cca agg 1219
Ser Tyr Ile Lys Ala Ala Lys Arg Leu Tyr Met Thr Ala Thr Pro Arg
360 365 370
ctt ttc gac gac tcc gtg aag ggc aag gct gca gat cat tca gct gaa 1267
Leu Phe Asp Asp Ser Val Lys Gly Lys Ala Ala Asp His Ser Ala Glu
375 380 385
gtt tct tcc atg gat gat gaa gca atc tac gga ccc gaa ttt cac cgg 1315
Val Ser Ser Met Asp Asp Glu Ala Ile Tyr Gly Pro Glu Phe His Arg
390 395 400 405
ctg ggt ttt ggg gaa gca gta gaa aaa ggc ctg ctt act gac tac aaa 1363
Leu Gly Phe Gly Glu Ala Val Glu Lys Gly Leu Leu Thr Asp Tyr Lys
410 415 420
gtt gta gtg atg aca gtt gat gag caa gtt gca gcc agt gcc tta act 1411
Val Val Val Met Thr Val Asp Glu Gln Val Ala Ala Ser Ala Leu Thr
425 430 435
gtt ttg ggt tca aca cct ggt gaa gaa ctc act ctg gac atg act tcc 1459
Val Leu Gly Ser Thr Pro Gly Glu Glu Leu Thr Leu Asp Met Thr Ser
440 445 450
gca att att ggc gcg tgg aat ggc ttg gca aag cgt tcg ggt aaa gag 1507
Ala Ile Ile Gly Ala Trp Asn Gly Leu Ala Lys Arg Ser Gly Lys Glu
455 460 465
caa gac acc aag act ggt ttc tca agc tct gac gca gca atg gaa cga 1555
Gln Asp Thr Lys Thr Gly Phe Ser Ser Ser Asp Ala Ala Met Glu Arg
470 475 480 485
gct gtt gca ttt gcg cga gac att aag act tct cag caa atc gcg gag 1603
Ala Val Ala Phe Ala Arg Asp Ile Lys Thr Ser Gln Gln Ile Ala Glu
490 495 500
tct ttt cca cga gtt gtc aat gct tat acg act gag ctt gag gtt aaa 1651
Ser Phe Pro Arg Val Val Asn Ala Tyr Thr Thr Glu Leu Glu Val Lys
505 510 515
aac gat gac gta gat gag cac aac ctc aat ttg agc gtg gca tgc cag 1699
Asn Asp Asp Val Asp Glu His Asn Leu Asn Leu Ser Val Ala Cys Gln
520 525 530
cat gtc gat gga tca atg aat gcg ctg gaa cga aac tct cgt tta act 1747
His Val Asp Gly Ser Met Asn Ala Leu Glu Arg Asn Ser Arg Leu Thr
535 540 545
tgg ctt aaa gct cct acc cag tca atg gaa acc aag att ctt act aac 1795
Trp Leu Lys Ala Pro Thr Gln Ser Met Glu Thr Lys Ile Leu Thr Asn
550 555 560 565
gcg cgc tgc ctt tct gag ggt gtc gat gta ccg gct ttg gat tcc gta 1843
Ala Arg Cys Leu Ser Glu Gly Val Asp Val Pro Ala Leu Asp Ser Val
570 575 580
atc ttc ttt aat ccc cgt aat tcc atg gtg gat gtg gtt cag tcg gtt 1891
Ile Phe Phe Asn Pro Arg Asn Ser Met Val Asp Val Val Gln Ser Val
585 590 595
ggc cga gtt atg cgt aaa tct cca ggt aag aat tac gga tat atc atc 1939
Gly Arg Val Met Arg Lys Ser Pro Gly Lys Asn Tyr Gly Tyr Ile Ile
600 605 610
ttg cca gtt gct gtt ccc cca ggt gtt gca cca tca gct gcg tta aat 1987
Leu Pro Val Ala Val Pro Pro Gly Val Ala Pro Ser Ala Ala Leu Asn
615 620 625
gat tca cgc cgc ttc aag gtt gta tgg cag gtt ctt aac gcc ctt cgt 2035
Asp Ser Arg Arg Phe Lys Val Val Trp Gln Val Leu Asn Ala Leu Arg
630 635 640 645
gct cac gat gac cgt ttt aac gca atg gtg aac tcc att gcg ctc aat 2083
Ala His Asp Asp Arg Phe Asn Ala Met Val Asn Ser Ile Ala Leu Asn
650 655 660
gag gga aat att aag gat cta cct gtt gag act gag cat act ggc cct 2131
Glu Gly Asn Ile Lys Asp Leu Pro Val Glu Thr Glu His Thr Gly Pro
665 670 675
aca agt aaa gat aga gat aac gca ccc tat gac agt gct gaa tcc gct 2179
Thr Ser Lys Asp Arg Asp Asn Ala Pro Tyr Asp Ser Ala Glu Ser Ala
680 685 690
aca caa tac gta ctg ttc tct tta gaa cag tgg caa gaa gcg atc tac 2227
Thr Gln Tyr Val Leu Phe Ser Leu Glu Gln Trp Gln Glu Ala Ile Tyr
695 700 705
acc aag ctt gta gat aaa gtt ggt acc cga acc tac tgg gaa gat tgg 2275
Thr Lys Leu Val Asp Lys Val Gly Thr Arg Thr Tyr Trp Glu Asp Trp
710 715 720 725
gca gat gat gtt gct gac atc gcc caa gca caa ata acc cgt att aaa 2323
Ala Asp Asp Val Ala Asp Ile Ala Gln Ala Gln Ile Thr Arg Ile Lys
730 735 740
gcg ctc tta gac aat gct tca cca acg atc aaa gaa gag ttc gag cga 2371
Ala Leu Leu Asp Asn Ala Ser Pro Thr Ile Lys Glu Glu Phe Glu Arg
745 750 755
ttt gtt gaa ggt cta cgt ggc aac ctc aat gag tcc atc tct gac gac 2419
Phe Val Glu Gly Leu Arg Gly Asn Leu Asn Glu Ser Ile Ser Asp Asp
760 765 770
gag gca atc agc atg ttg tca cag cat ctg atc acc gca cca gtg ttt 2467
Glu Ala Ile Ser Met Leu Ser Gln His Leu Ile Thr Ala Pro Val Phe
775 780 785
gac gct cta ttt gct gaa tca agc ttc gct aag caa aac cct gtt tcc 2515
Asp Ala Leu Phe Ala Glu Ser Ser Phe Ala Lys Gln Asn Pro Val Ser
790 795 800 805
cag gtt atg caa cgc atg gca gat gct ctt aat agt gct gaa ctt aac 2563
Gln Val Met Gln Arg Met Ala Asp Ala Leu Asn Ser Ala Glu Leu Asn
810 815 820
tct gaa acg gaa aaa ctt gag aag ttc tat gac tct gtt cgt atc cgt 2611
Ser Glu Thr Glu Lys Leu Glu Lys Phe Tyr Asp Ser Val Arg Ile Arg
825 830 835
gct gct gaa gta agc tcc gca gct ggt aaa caa gca gta att aaa gac 2659
Ala Ala Glu Val Ser Ser Ala Ala Gly Lys Gln Ala Val Ile Lys Asp
840 845 850
ctc tac gaa cga ttc ttt aaa aag gcc ttt aaa aag caa tct gaa gct 2707
Leu Tyr Glu Arg Phe Phe Lys Lys Ala Phe Lys Lys Gln Ser Glu Ala
855 860 865
cta ggt att gtc tat acc cct gtt gag atc gtg gac ttt att ctg cga 2755
Leu Gly Ile Val Tyr Thr Pro Val Glu Ile Val Asp Phe Ile Leu Arg
870 875 880 885
gct gcc gat gat gtg tcc aag aag cat ttt ggt cgt ggc tta agc gat 2803
Ala Ala Asp Asp Val Ser Lys Lys His Phe Gly Arg Gly Leu Ser Asp
890 895 900
aaa gat gtc cat gtt ctt gat cct ttc acc ggt acg ggt act ttt atg 2851
Lys Asp Val His Val Leu Asp Pro Phe Thr Gly Thr Gly Thr Phe Met
905 910 915
gtg cgt tta ttg cag tca ggt cta att aag cct gaa gat ttg gcc cgt 2899
Val Arg Leu Leu Gln Ser Gly Leu Ile Lys Pro Glu Asp Leu Ala Arg
920 925 930
aaa tat gcc aat gag ctg cac gct act gag atc atg ttg ctt gcc tat 2947
Lys Tyr Ala Asn Glu Leu His Ala Thr Glu Ile Met Leu Leu Ala Tyr
935 940 945
tat gtt gcg gcc gtt aac att gag acc act tat ttt ggt ctc gag gga 2995
Tyr Val Ala Ala Val Asn Ile Glu Thr Thr Tyr Phe Gly Leu Glu Gly
950 955 960 965
gag cgt gct ctg cgt aat ggt gaa gat gcg ccg gtc tat gag ccg ttt 3043
Glu Arg Ala Leu Arg Asn Gly Glu Asp Ala Pro Val Tyr Glu Pro Phe
970 975 980
gat ggc att gtg ttg ggt gat acc ttc cag atg tat gaa gac gat gac 3091
Asp Gly Ile Val Leu Gly Asp Thr Phe Gln Met Tyr Glu Asp Asp Asp
985 990 995
aaa ctc gat cta gat gtt ttt act gct aac aat gac cgt atg gag cgt 3139
Lys Leu Asp Leu Asp Val Phe Thr Ala Asn Asn Asp Arg Met Glu Arg
1000 1005 1010
cag aga ctt act cct gta cag gtt att gtg ggt aac ccg cct tac tct 3187
Gln Arg Leu Thr Pro Val Gln Val Ile Val Gly Asn Pro Pro Tyr Ser
1015 1020 1025
gtg ggg caa tcg agt gcg aat gac aat aac gca aac ctc aag tac ccc 3235
Val Gly Gln Ser Ser Ala Asn Asp Asn Asn Ala Asn Leu Lys Tyr Pro
1030 1035 1040 1045
act ctt gat cga cgt att gaa gat tct tat gcg aag tat tcg acc gca 3283
Thr Leu Asp Arg Arg Ile Glu Asp Ser Tyr Ala Lys Tyr Ser Thr Ala
1050 1055 1060
aca aat aag aac tct ctg tac gac tcc tat tta cgt gct ttc cgg tgg 3331
Thr Asn Lys Asn Ser Leu Tyr Asp Ser Tyr Leu Arg Ala Phe Arg Trp
1065 1070 1075
gca aca gat cgt att cac aca caa ggg gtt gtt gct ttt gtt tct aac 3379
Ala Thr Asp Arg Ile His Thr Gln Gly Val Val Ala Phe Val Ser Asn
1080 1085 1090
aac ggt tgg gtt gac ggc aat acc gct gat ggt gtg cgc cta agt ttg 3427
Asn Gly Trp Val Asp Gly Asn Thr Ala Asp Gly Val Arg Leu Ser Leu
1095 1100 1105
gca cag gac ttc tcc gag att tat gtt ttc aac ctt cgt ggc aat tcc 3475
Ala Gln Asp Phe Ser Glu Ile Tyr Val Phe Asn Leu Arg Gly Asn Ser
1110 1115 1120 1125
cga aca ggt ggt gat ttg gct aag cgc gag ggc ggc aac gtc ttt aat 3523
Arg Thr Gly Gly Asp Leu Ala Lys Arg Glu Gly Gly Asn Val Phe Asn
1130 1135 1140
gtc cgt gtg ggt act caa atc att gtt gcc gtg aaa aac cca caa ttg 3571
Val Arg Val Gly Thr Gln Ile Ile Val Ala Val Lys Asn Pro Gln Leu
1145 1150 1155
tct ggt tgc agg att ctt tat aaa gat att ggt gac aat ctc agc gcg 3619
Ser Gly Cys Arg Ile Leu Tyr Lys Asp Ile Gly Asp Asn Leu Ser Ala
1160 1165 1170
gat gcg aag ttg aac gag att gcc gtt gcc aca att gag ggc gct gag 3667
Asp Ala Lys Leu Asn Glu Ile Ala Val Ala Thr Ile Glu Gly Ala Glu
1175 1180 1185
tgg cag act att tcg cct aat gaa tat gga gat tgg atc agc cag cgc 3715
Trp Gln Thr Ile Ser Pro Asn Glu Tyr Gly Asp Trp Ile Ser Gln Arg
1190 1195 1200 1205
tct gta gat ttt gat acc tgg cct gta ctc ggt gat aag aaa aac aaa 3763
Ser Val Asp Phe Asp Thr Trp Pro Val Leu Gly Asp Lys Lys Asn Lys
1210 1215 1220
tct gca tta aag gtt ttt caa acc ttt tct gct ggt cta aag act ggc 3811
Ser Ala Leu Lys Val Phe Gln Thr Phe Ser Ala Gly Leu Lys Thr Gly
1225 1230 1235
cgc gat gcc tgg tgc tat ggg cca aca tct gcg cag gta aaa act aat 3859
Arg Asp Ala Trp Cys Tyr Gly Pro Thr Ser Ala Gln Val Lys Thr Asn
1240 1245 1250
atc acc cgg tta tta gaa acc tat gaa caa gct cag caa cgg ttt aat 3907
Ile Thr Arg Leu Leu Glu Thr Tyr Glu Gln Ala Gln Gln Arg Phe Asn
1255 1260 1265
tcg tgg gtt gtg gat aac gga gtg aca tct cct aag gaa gct gac gtt 3955
Ser Trp Val Val Asp Asn Gly Val Thr Ser Pro Lys Glu Ala Asp Val
1270 1275 1280 1285
aat caa ttc ctt aag cag aac cct gat tta gcg gat agc aag aaa ata 4003
Asn Gln Phe Leu Lys Gln Asn Pro Asp Leu Ala Asp Ser Lys Lys Ile
1290 1295 1300
tcc tgg gat tcc aat cta aaa atg tcc ttg tca cgc ggt gat act ttt 405l
Ser Trp Asp Ser Asn Leu Lys Met Ser Leu Ser Arg Gly Asp Thr Phe
1305 1310 1315
tct ttt gat cca agc agc atc caa atg tcc ttg tat cgt cca ttt ttc 4099
Ser Phe Asp Pro Ser Ser Ile Gln Met Ser Leu Tyr Arg Pro Phe Phe
1320 1325 1330
cct caa cag aca tac ttc cat gtt tca ttg aac cag cgt cga tac cag 4147
Pro Gln Gln Thr Tyr Phe His Val Ser Leu Asn Gln Arg Arg Tyr Gln
1335 1340 1345
tta ccg tca atg ttc ccg acc ccg gaa cat gac aac cag ggc ttt tat 4195
Leu Pro Ser Met Phe Pro Thr Pro Glu His Asp Asn Gln Gly Phe Tyr
1350 1355 1360 1365
atc gtc aac cca ggt agt gcc aag cca ttc tct acc ctt gca aca aat 4243
Ile Val Asn Pro Gly Ser Ala Lys Pro Phe Ser Thr Leu Ala Thr Asn
1370 1375 1380
cta ctt cca gat ctt gct atg tgg ggt tct aac gcc gga cag ttc ttt 4291
Leu Leu Pro Asp Leu Ala Met Trp Gly Ser Asn Ala Gly Gln Phe Phe
1385 1390 1395
acc cga tgg act tgg gaa ccc atc gaa act cga gaa ggc gaa tta gac 4339
Thr Arg Trp Thr Trp Glu Pro Ile Glu Thr Arg Glu Gly Glu Leu Asp
1400 1405 1410
ttc ggt aat gga ctt ttc tcc aca acc cca aaa aag gga gtt gaa ggc 4387
Phe Gly Asn Gly Leu Phe Ser Thr Thr Pro Lys Lys Gly Val Glu Gly
1415 1420 1425
gag atc ctt gac ggt tac cgg cgc gtc gat aac atc acc gac gag atc 4435
Glu Ile Leu Asp Gly Tyr Arg Arg Val Asp Asn Ile Thr Asp Glu Ile
1430 1435 1440 1445
tta aag ctc tac caa tca agc ttg ggt gag gat gtc acc aag gat gac 4483
Leu Lys Leu Tyr Gln Ser Ser Leu Gly Glu Asp Val Thr Lys Asp Asp
1450 1455 1460
atc ttc tac ttt gtt tat gca cag ttg cat gat cct gcc tat cgt gag 4531
Ile Phe Tyr Phe Val Tyr Ala Gln Leu His Asp Pro Ala Tyr Arg Glu
1465 1470 1475
gcc tat gcg gct gat cta aag aag atg ctg cca cat att gaa acc cct 4579
Ala Tyr Ala Ala Asp Leu Lys Lys Met Leu Pro His Ile Glu Thr Pro
1480 1485 1490
act gat cgc gca cgc ttt gat cac ttt gtg aca gcc ggc aag gaa ttg 4627
Thr Asp Arg Ala Arg Phe Asp His Phe Val Thr Ala Gly Lys Glu Leu
1495 1500 1505
atg gat ctt cat atc aac tat gaa gat gtt gaa cca tgg gat gtg gag 4675
Met Asp Leu His Ile Asn Tyr Glu Asp Val Glu Pro Trp Asp Val Glu
1510 1515 1520 1525
gta aag gtc aaa gaa aaa gct gat ccc acg gat cgt gag acc tgg cgt 4723
Val Lys Val Lys Glu Lys Ala Asp Pro Thr Asp Arg Glu Thr Trp Arg
1530 1535 1540
gtt acc aag atg aag tgg gct aag gtt cgt gat cca gag acc aag aaa 4771
Val Thr Lys Met Lys Trp Ala Lys Val Arg Asp Pro Glu Thr Lys Lys
1545 1550 1555
ttg gtg gag gac cat acc acc ttg att tac aac agc tca atc acc atc 4819
Leu Val Glu Asp His Thr Thr Leu Ile Tyr Asn Ser Ser Ile Thr Ile
1560 1565 1570
agc ggc atc ccc gaa gaa gct gaa aac tat caa cta ggt tca cgt tct 4867
Ser Gly Ile Pro Glu Glu Ala Glu Asn Tyr Gln Leu Gly Ser Arg Ser
1575 1580 1585
gcc atc gca tgg ctt att gac cgt tac cag gtg aag aaa gat aag gcg 4915
Ala Ile Ala Trp Leu Ile Asp Arg Tyr Gln Val Lys Lys Asp Lys Ala
1590 1595 1600 1605
tcc ggc att gtt aat gat ccc aat gat tgg gct gat gaa gtg ggt aac 4963
Ser Gly Ile Val Asn Asp Pro Asn Asp Trp Ala Asp Glu Val Gly Asn
1610 1615 1620
cct cgc tac att gtg gag ctg att gct aag gtc acc cgc gtt gcc gtt 5011
Pro Arg Tyr Ile Val Glu Leu Ile Ala Lys Val Thr Arg Val Ala Val
1625 1630 1635
gag acc atg aga att gta gag gag ctt tgagttttcc tactgcagct 5058
Glu Thr Met Arg Ile Val Glu Glu Leu
1640 1645
tccagaagtg 5068
<210>46
<211>1646
<212>PRT
<213>谷氨酸棒状杆菌
<400>46
Met Glu Cys Met Ser Thr Ile Thr Asp Ala Val Ser Ala Leu Arg Thr
1 5 10 15
Leu Pro Ser Gln Ser Ala Gln Gly Leu Ala Phe Glu Lys Leu Met Val
20 25 30
Asn Phe Ile Lys Ser Asp Pro Thr Leu Ser Thr Glu Phe Asp Glu Val
35 40 45
His Arg Trp Val Asp Trp Pro Tyr Asn Gly Gly Thr Met Asp Thr Gly
50 55 60
Ile Asp Leu Val Ala Tyr Asn Lys Asp Asp Asp Ala Tyr Thr Ala Ile
65 70 75 80
Gln Cys Lys Phe Tyr Leu Pro Thr Thr Ser Leu Ala Lys Gly Gln Leu
85 90 95
Asp Ser Phe Phe Glu Ala Ser Gly Arg Thr Phe Glu Thr Pro Glu Gly
100 105 110
Thr Arg Ser Phe Ser Asn Arg Leu Val Ile Ser Thr Thr Asp Lys Trp
115 120 125
Ser Ser Asn Ala Glu Lys Met Leu Glu Asn Gln Thr Ile Pro Thr Asn
130 135 140
Arg Ile Gly Leu Ser Ala Ile Ala Glu Ser Pro Ile Asp Trp Asp Ile
145 150 155 160
Ala Tyr pro Gly Ser Glu Leu Thr Ile Asn Leu Gln Leu Lys Glu Pro
165 170 175
Tyr Ser Pro Arg Pro His Gln Gln Thr Ala Ile Glu Lys Ala Ile Glu
180 185 190
Gly Phe Gln Thr His Asp Arg Gly Lys Leu Ile Met Ala Cys Gly Thr
195 200 205
Gly Lys Thr Phe Thr Ala Leu Arg Leu Ser Glu Glu Val Ala Arg Leu
210 215 220
Asn Gly Asn Lys Ala Arg Ile Leu Phe Leu Val Pro Ser Ile Ser Leu
225 230 235 240
Leu Ser Gln Thr Leu Lys Glu Trp Thr Ala Gln Lys Thr Met Asp Leu
245 250 255
Arg Pro Val Ala Val Cys Ser Asp Ser Lys Val Ser Lys Ala Ala Glu
260 265 270
Asp Ile Ala Ala Tyr Asp Leu Glu Val Pro Val Ser Thr Asp Gly Ala
275 280 285
Leu Ile Ala Glu Lys Leu Glu His Arg Lys Arg Ala Ala Gly Leu Thr
290 295 300
Val Val Phe Ser Thr Tyr Gln Ser Leu Pro Ala Val His Ala Ala Gln
305 310 315 320
Glu Ala Gly Ala Glu Pro Phe Asp Leu Val Ile Cys Asp Glu Ala His
325 330 335
Arg Thr Thr Gly Ile Thr Leu Ala Gly Glu Asp pro Ser Asn Phe Thr
340 345 350
Arg Ile His Asp Ala Ser Tyr Ile Lys Ala Ala Lys Arg Leu Tyr Met
355 360 365
Thr Ala Thr Pro Arg Leu Phe Asp Asp Ser Val Lys Gly Lys Ala Ala
370 375 380
Asp His Ser Ala Glu Val Ser Ser Met Asp Asp Glu Ala Ile Tyr Gly
385 390 395 400
Pro Glu Phe His Arg Leu Gly Phe Gly Glu Ala Val Glu Lys Gly Leu
405 410 415
Leu Thr Asp Tyr Lys Val Val Val Met Thr Val Asp Glu Gln Val Ala
420 425 430
Ala Ser Ala Leu Thr Val Leu Gly Ser Thr Pro Gly Glu Glu Leu Thr
435 440 445
Leu Asp Met Thr Ser Ala Ile Ile Gly Ala Trp Asn Gly Leu Ala Lys
450 455 460
Arg Ser Gly Lys Glu Gln Asp Thr Lys Thr Gly Phe Ser Ser Ser Asp
465 470 475 480
Ala Ala Met Glu Arg Ala Val Ala Phe Ala Arg Asp Ile Lys Thr Ser
485 490 495
Gln Gln Ile Ala Glu Ser Phe Pro Arg Val Val Asn Ala Tyr Thr Thr
500 505 510
Glu Leu Glu Val Lys Asn Asp Asp Val Asp Glu His Asn Leu Asn Leu
515 520 525
Ser Val Ala Cys Gln His Val Asp Gly Ser Met Asn Ala Leu Glu Arg
530 535 540
Asn Ser Arg Leu Thr Trp Leu Lys Ala Pro Thr Gln Ser Met Glu Thr
545 550 555 560
Lys Ile Leu Thr Asn Ala Arg Cys Leu Ser Glu Gly Val Asp Val Pro
565 570 575
Ala Leu Asp Ser Val Ile Phe Phe Asn Pro Arg Asn Ser Met Val Asp
580 585 590
Val Val Gln Ser Val Gly Arg Val Met Arg Lys Ser Pro Gly Lys Asn
595 600 605
Tyr Gly Tyr Ile Ile Leu Pro Val Ala Val Pro Pro Gly Val Ala Pro
610 615 620
Ser Ala Ala Leu Asn Asp Ser Arg Arg Phe Lys Val Val Trp Gln Val
625 630 635 640
Leu Asn Ala Leu Arg Ala His Asp Asp Arg Phe Asn Ala Met Val Asn
645 650 655
Ser Ile Ala Leu Asn Glu Gly Asn Ile Lys Asp Leu Pro Val Glu Thr
660 665 670
Glu His Thr Gly Pro Thr Ser Lys Asp Arg Asp Asn Ala Pro Tyr Asp
675 680 685
Ser Ala Glu Ser Ala Thr Gln Tyr Val Leu Phe Ser Leu Glu Gln Trp
690 695 700
Gln Glu Ala Ile Tyr Thr Lys Leu Val Asp Lys Val Gly Thr Arg Thr
705 710 715 720
Tyr Trp Glu Asp Trp Ala Asp Asp Val Ala Asp Ile Ala Gln Ala Gln
725 730 735
Ile Thr Arg Ile Lys Ala Leu Leu Asp Asn Ala Ser Pro Thr Ile Lys
740 745 750
Glu Glu Phe Glu Arg Phe Val Glu Gly Leu Arg Gly Asn Leu Asn Glu
755 760 765
Ser Ile Ser Asp Asp Glu Ala Ile Ser Met Leu Ser Gln His Leu Ile
770 775 780
Thr Ala Pro Val Phe Asp Ala Leu Phe Ala Glu Ser Ser Phe Ala Lys
785 790 795 800
Gln Asn Pro Val Ser Gln Val Met Gln Arg Met Ala Asp Ala Leu Asn
805 810 815
Ser Ala Glu Leu Asn Ser Glu Thr Glu Lys Leu Glu Lys Phe Tyr Asp
820 825 830
Ser Val Arg Ile Arg Ala Ala Glu Val Ser Ser Ala Ala Gly Lys Gln
835 840 845
Ala Val Ile Lys Asp Leu Tyr Glu Arg Phe Phe Lys Lys Ala Phe Lys
850 855 860
Lys Gln Ser Glu Ala Leu Gly Ile Val Tyr Thr Pro Val Glu Ile Val
865 870 875 880
Asp Phe Ile Leu Arg Ala Ala Asp Asp Val Ser Lys Lys His Phe Gly
885 890 895
Arg Gly Leu Ser Asp Lys Asp Val His Val Leu Asp Pro Phe Thr Gly
900 905 910
Thr Gly Thr Phe Met Val Arg Leu Leu Gln Ser Gly Leu Ile Lys Pro
915 920 925
Glu Asp Leu Ala Arg Lys Tyr Ala Asn Glu Leu His Ala Thr Glu Ile
930 935 940
Met Leu Leu Ala Tyr Tyr Val Ala Ala Val Asn Ile Glu Thr Thr Tyr
945 950 955 960
Phe Gly Leu Glu Gly Glu Arg Ala Leu Arg Asn Gly Glu Asp Ala Pro
965 970 975
Val Tyr Glu Pro Phe Asp Gly Ile Val Leu Gly Asp Thr Phe Gln Met
980 985 990
Tyr Glu Asp Asp Asp Lys Leu Asp Leu Asp Val Phe Thr Ala Asn Asn
995 1000 1005
Asp Arg Met Glu Arg Gln Arg Leu Thr Pro Val Gln Val Ile Val Gly
1010 1015 1020
Asn Pro Pro Tyr Ser Val Gly Gln Ser Ser Ala Asn Asp Asn Asn Ala
1025 1030 1035 1040
Asn Leu Lys Tyr Pro Thr Leu Asp Arg Arg Ile Glu Asp Ser Tyr Ala
1045 1050 1055
Lys Tyr Ser Thr Ala Thr Asn Lys Asn Ser Leu Tyr Asp Ser Tyr Leu
1060 1065 1070
Arg Ala Phe Arg Trp Ala Thr Asp Arg Ile His Thr Gln Gly Val Val
1075 1080 1085
Ala Phe Val Ser Asn Asn Gly Trp Val Asp Gly Asn Thr Ala Asp Gly
1090 1095 1100
Val Arg Leu Ser Leu Ala Gln Asp Phe Ser Glu Ile Tyr Val Phe Asn
1105 1110 1115 1120
Leu Arg Gly Asn Ser Arg Thr Gly Gly Asp Leu Ala Lys Arg Glu Gly
1125 1130 1135
Gly Asn Val Phe Asn Val Arg Val Gly Thr Gln Ile Ile Val Ala Val
1140 1145 1150
Lys Asn Pro Gln Leu Ser Gly Cys Arg Ile Leu Tyr Lys Asp Ile Gly
1155 1160 1165
Asp Asn Leu Ser Ala Asp Ala Lys Leu Asn Glu Ile Ala Val Ala Thr
1170 1175 1180
Ile Glu Gly Ala Glu Trp Gln Thr Ile Ser Pro Asn Glu Tyr Gly Asp
1185 1190 1195 1200
Trp Ile Ser Gln Arg Ser Val Asp Phe Asp Thr Trp Pro Val Leu Gly
1205 1210 1215
Asp Lys Lys Asn Lys Ser Ala Leu Lys Val Phe Gln Thr Phe Ser Ala
1220 1225 1230
Gly Leu Lys Thr Gly Arg Asp Ala Trp Cys Tyr Gly Pro Thr Ser Ala
1235 1240 1245
Gln Val Lys Thr Asn Ile Thr Arg Leu Leu Glu Thr Tyr Glu Gln Ala
1250 1255 1260
Gln Gln Arg Phe Asn Ser Trp Val Val Asp Asn Gly Val Thr Ser Pro
1265 1270 1275 1280
Lys Glu Ala Asp Val Asn Gln Phe Leu Lys Gln Asn Pro Asp Leu Ala
1285 1290 1295
Asp Ser Lys Lys Ile Ser Trp Asp Ser Asn Leu Lys Met Ser Leu Ser
1300 1305 1310
Arg Gly Asp Thr Phe Ser Phe Asp Pro Ser Ser Ile Gln Met Ser Leu
1315 1320 1325
Tyr Arg Pro Phe Phe Pro Gln Gln Thr Tyr Phe His Val Ser Leu Asn
1330 1335 1340
Gln Arg Arg Tyr Gln Leu Pro Ser Met Phe Pro Thr Pro Glu His Asp
1345 1350 1355 1360
Asn Gln Gly Phe Tyr Ile Val Asn Pro Gly Ser Ala Lys Pro Phe Ser
1365 1370 1375
Thr Leu Ala Thr Asn Leu Leu Pro Asp Leu Ala Met Trp Gly Ser Asn
1380 1385 1390
Ala Gly Gln Phe Phe Thr Arg Trp Thr Trp Glu Pro Ile Glu Thr Arg
1395 1400 1405
Glu Gly Glu Leu Asp Phe Gly Asn Gly Leu Phe Ser Thr Thr Pro Lys
1410 1415 1420
Lys Gly Val Glu Gly Glu Ile Leu Asp Gly Tyr Arg Arg Val Asp Asn
1425 1430 1435 1440
Ile Thr Asp Glu Ile Leu Lys Leu Tyr Gln Ser Ser Leu Gly Glu Asp
1445 1450 1455
Val Thr Lys Asp Asp Ile Phe Tyr Phe Val Tyr Ala Gln Leu His Asp
1460 1465 1470
Pro Ala Tyr Arg Glu Ala Tyr Ala Ala Asp Leu Lys Lys Met Leu Pro
1475 1480 1485
His Ile Glu Thr Pro Thr Asp Arg Ala Arg Phe Asp His Phe Val Thr
1490 1495 1500
Ala Gly Lys Glu Leu Met Asp Leu His Ile Asn Tyr Glu Asp Val Glu
1505 1510 1515 1520
Pro Trp Asp Val Glu Val Lys Val Lys Glu Lys Ala Asp Pro Thr Asp
1525 1530 1535
Arg Glu Thr Trp Arg Val Thr Lys Met Lys Trp Ala Lys Val Arg Asp
1540 1545 1550
Pro Glu Thr Lys Lys Leu Val Glu Asp His Thr Thr Leu Ile Tyr Asn
1555 1560 1565
Ser Ser Ile Thr Ile Ser Gly Ile Pro Glu Glu Ala Glu Asn Tyr Gln
1570 1575 1580
Leu Gly Ser Arg Ser Ala Ile Ala Trp Leu Ile Asp Arg Tyr Gln Val
1585 1590 1595 1600
Lys Lys Asp Lys Ala Ser Gly Ile Val Asn Asp Pro Asn Asp Trp Ala
1605 1610 1615
Asp Glu Val Gly Asn Pro Arg Tyr Ile Val Glu Leu Ile Ala Lys Val
1620 1625 1630
Thr Arg Val Ala Val Glu Thr Met Arg Ile Val Glu Glu Leu
1635 1640 1645
<210>47
<211>433
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(403)
<223>RXA01949
<400>47
cctacgacgt tctcaagtct gacgacgttg tgttetccgt tgaggctctc cacaccttca 60
tcaaccgcgc ttccggtgcg gcacaggagg agcagaacta atg gct act atc gcc 115
Met Ala Thr Ile Ala
1 5
aac cca cgc gac atc atc atc gca ccg gtc gtt tct gag aag tcc tac 163
Asn Pro Arg Asp Ile Ile Ile Ala Pro Val Val Ser Glu Lys Ser Tyr
10 15 20
ggc ctc atg gag cag aac gtt tac acg ttc ttc gtc tcc act gac gct 211
Gly Leu Met Glu Gln Asn Val Tyr Thr Phe Phe Val Ser Thr Asp Ala
25 30 35
aac aag act cag atc aag att gcc atc gaa gag atc ttc ggc gtc aag 259
Asn Lys Thr Gln Ile Lys Ile Ala Ile Glu Glu Ile Phe Gly Val Lys
40 45 50
gtt gca tct gtg aac acc gtt aac cgt gca ggt aag cgc aag cgc tcc 307
Val Ala Ser Val Asn Thr Val Asn Arg Ala Gly Lys Arg Lys Arg Ser
55 60 65
cgc acc ggc ttc ggt act cgc aag gct acc aag cgc gct tat gtg act 355
Arg Thr Gly Phe Gly Thr Arg Lys Ala Thr Lys Arg Ala Tyr Val Thr
70 75 80 85
ctt cgc gaa ggc agc gac tcc atc gac ate ttc agc ggc tcc gtc gct 403
Leu Arg Glu Gly Ser Asp Ser Ile Asp Ile Phe Ser Gly Ser Val Ala
90 95 100
taagacgtcg atagaaaagg acacattatg 433
<210>48
<211>101
<212>PRT
<213>谷氨酸棒状杆菌
<400>48
Met Ala Thr Ile Ala Asn Pro Arg Asp Ile Ile Ile Ala Pro Val Val
l 5 10 15
Ser Glu Lys Ser Tyr Gly Leu Met Glu Gln Asn Val Tyr Thr Phe Phe
20 25 30
Val Ser Thr Asp Ala Asn Lys Thr Gln Ile Lys Ile Ala Ile Glu Glu
35 40 45
Ile Phe Gly Val Lys Val Ala Ser Val Asn Thr Val Asn Arg Ala Gly
50 55 60
Lys Arg Lys Arg Ser Arg Thr Gly Phe Gly Thr Arg Lys Ala Thr Lys
65 70 75 80
Arg Ala Tyr Val Thr Leu Arg Glu Gly Ser Asp Ser Ile Asp Ile Phe
85 90 95
Ser Gly Ser Val Ala
100
<210>49
<211>787
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(757)
<223>RXA01966
<400>49
gctgtccaag gccatgaatt ttgtgtcctc tgttgctcca accgctgtgc tctcccctgt 60
tatgggctgg gtttataaga agatgggtta gtttttaaaa gtg tct gaa tct gaa 115
Val Ser Glu Ser Glu
1 5
aac aac aca aca cca gca gtc gca gct cgc gat gac cgt ctg gtc tgg 163
Asn Asn Thr Thr Pro Ala Val Ala Ala Arg Asp Asp Arg Leu Val Trp
10 15 20
gtc gat ctg gaa atg act ggt cta gat ttg aag cgc cac gtg atc gtg 211
Val Asp Leu Glu Met Thr Gly Leu Asp Leu Lys Arg His Val Ile Val
25 30 35
gag gtt gcg gcg ttg gtc act gac gct aac ctc aac gtt ttg ggc gag 259
Glu Val Ala Ala Leu Val Thr Asp Ala Asn Leu Asn Val Leu Gly Glu
40 45 50
ggc gtg gac ttg gtt gtt cac gca act gaa gaa gag ctc gcg cag atg 307
Gly Val Asp Leu Val Val His Ala Thr Glu Glu Glu Leu Ala Gln Met
55 60 65
gat gat ttt gtc acc aac atg cac gaa tcc tct ggg ctg act gag cag 355
Asp Asp Phe Val Thr Asn Met His Glu Ser Ser Gly Leu Thr Glu Gln
70 75 80 85
atc cgg gaa tcc gcg gtc acg ttg aag gaa gcc gaa gat gct gtg ctc 403
Ile Arg Glu Ser Ala Val Thr Leu Lys Glu Ala Glu Asp Ala Val Leu
90 95 100
gca ttg att gaa aag cac tgc gat cca gcc cat cct gca ccg cta gct 451
Ala Leu Ile Glu Lys His Cys Asp Pro Ala His Pro Ala Pro Leu Ala
105 110 115
ggt aac tcc att gcc act gac cgc gcg ttt atc cgc gaa cat atg cea 499
Gly Asn Ser Ile Ala Thr Asp Arg Ala Phe Ile Arg Glu His Met Pro
120 125 130
cgt ctt gat gag gcc ctg cat tac cgc atg gtg gat gtg tcc tcg gtg 547
Arg Leu Asp Glu Ala Leu His Tyr Arg Met Val Asp Val Ser Ser Val
135 140 145
aag gaa ttg gcg cgt cgc tgg tac cca cgc gtg tac tac aag cag ccg 595
Lys Glu Leu Ala Arg Arg Trp Tyr Pro Arg Val Tyr Tyr Lys Gln Pro
150 155 160 165
gag aag ggt ttg gcg cac cgc gcg ttg gcg gac att gtg gag tcg att 643
Glu Lys Gly Leu Ala His Arg Ala Leu Ala Asp Ile Val Glu Ser Ile
170 175 180
cgg gag ttg gat tac tac cgt cgc tca ttt ttt gtt gca gag cct ggt 691
Arg Glu Leu Asp Tyr Tyr Arg Arg Ser Phe Phe Val Ala Glu Pro Gly
185 190 195
cct acc tct gag cag tgc gca gat gat gcg cag gca gcg gtg gac cgt 739
Pro Thr Ser Glu Gln Cys Ala Asp Asp Ala Gln Ala Ala Val Asp Arg
200 205 210
ttt gca ccc tac ttt gat tagagggttt taagcagcct ggtgagttcg 787
Phe Ala Pro Tyr Phe Asp
215
<210> 50
<211> 219
<212>PRT
<213>谷氨酸棒状杆菌
<400>50
Val Ser Glu Ser Glu Asn Asn Thr Thr Pro Ala Val Ala Ala Arg Asp
1 5 10 15
Asp Arg Leu Val Trp Val Asp Leu Glu Met Thr Gly Leu Asp Leu Lys
20 25 30
Arg His Val Ile Val Glu Val Ala Ala Leu Val Thr Asp Ala Asn Leu
35 40 45
Asn Val Leu Gly Glu Gly Val Asp Leu Val Val His Ala Thr Glu Glu
50 55 60
Glu Leu Ala Gln Met Asp Asp Phe Val Thr Asn Met His Glu Ser Ser
65 70 75 80
Gly Leu Thr Glu Gln Ile Arg Glu Ser Ala Val Thr Leu Lys Glu Ala
85 90 95
Glu Asp Ala Val Leu Ala Leu Ile Glu Lys His Cys Asp Pro Ala His
100 105 110
Pro Ala Pro Leu Ala Gly Asn Ser Ile Ala Thr Asp Arg Ala Phe Ile
115 120 125
Arg Glu His Met Pro Arg Leu Asp Glu Ala Leu His Tyr Arg Met Val
130 135 140
Asp Val Ser Ser Val Lys Glu Leu Ala Arg Arg Trp Tyr Pro Arg Val
145 150 155 160
Tyr Tyr Lys Gln Pro Glu Lys Gly Leu Ala His Arg Ala Leu Ala Asp
165 170 175
Ile Val Glu Ser Ile Arg Glu Leu Asp Tyr Tyr Arg Arg Ser Phe Phe
180 185 190
Val Ala Glu Pro Gly Pro Thr Ser Glu Gln Cys Ala Asp Asp Ala Gln
195 200 205
Ala Ala Val Asp Arg Phe Ala Pro Tyr Phe Asp
210 215
<210>51
<211>1255
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1225)
<223>RXA02070
<400>51
aacagcacgg atcaggacct caagatttgt ctgaccccac tcagggtgat gttgcagacg 60
aattttaggg agtatcaaaa cttgggacta ctatcgatac gtg atg act caa gta 115
Val Met Thr Gln Val
1 5
acc gaa tcc gct gtc cgc agc gcg cta tcc cgc gta gag gat cca gag 163
Thr Glu Ser Ala Val Arg Ser Ala Leu Ser Arg Val Glu Asp Pro Glu
10 15 20
atc ggt aag ccc atc aca gag ctc ggc atg gtc aaa tca gtg tcc atc 211
Ile Gly Lys Pro Ile Thr Glu Leu Gly Met Val Lys Ser Val Ser Ile
25 30 35
gac ggc tct gat gtc cag gtg gag gtc tac ctg acg atc gcg gct tgc 259
Asp Gly Ser Asp Val Gln Val Glu Val Tyr Leu Thr Ile Ala Ala Cys
40 45 50
ccg atg aaa acc acc att gtc acc aac act gaa gca gct ctc aaa gac 307
Pro Met Lys Thr Thr Ile Val Thr Asn Thr Glu Ala Ala Leu Lys Asp
55 60 65
atc gac ggg gtt ggc caa gtt cat gtc acc acc gat gtc atg agt gat 355
Ile Asp Gly Val Gly Gln Val His Val Thr Thr Asp Val Met Ser Asp
70 75 80 85
gaa cag cgc cgt gcg ctc cgc gtc tcc ctg cgc ggt gaa act tct gag 403
Glu Gln Arg Arg Ala Leu Arg Val Ser Leu Arg Gly Glu Thr Ser Glu
90 95 100
cca gtg att cca ttc gct cag cct ggt tcc act acc cgc gtt tac gct 451
Pro Val Ile Pro Phe Ala Gln Pro Gly Ser Thr Thr Arg Val Tyr Ala
105 110 115
gtt gct tcc ggc aaa ggt ggc gta gga aaa tcc tcc atg acg gtg aac 499
Val Ala Ser Gly Lys Gly Gly Val Gly Lys Ser Ser Met Thr Val Asn
120 125 130
ttg gct gca gcc cta gcc aag cgc ggg ctg tct gtg gga att ttg gat 547
Leu Ala Ala Ala Leu Ala Lys Arg Gly Leu Ser Val Gly Ile Leu Asp
135 140 145
gcc gat att tac gga cac tca gtg ccc gga atg ctc ggc tcg gac caa 595
Ala Asp Ile Tyr Gly His Ser Val Pro Gly Met Leu Gly Ser Asp Gln
150 155 160 165
cgc cca cac cag gtc gat gac atg atc atg cct ccc cag gcg cac ggc 643
Arg Pro His Gln Val Asp Asp Met Ile Met Pro Pro Gln Ala His Gly
170 175 180
gtg aag atg ata tcc att gct cac ttc acc gaa gga aat gct cct gtg 69l
Val Lys Met Ile Ser Ile Ala His Phe Thr Glu Gly Asn Ala Pro Val
185 190 195
gtg tgg cgt gga cca atg ctg cac cgt gcc atc cag caa ttc ctc act 739
Val Trp Arg Gly Pro Met Leu His Arg Ala Ile Gln Gln Phe Leu Thr
200 205 210
gac gtg ttc tgg ggc gac ctg gat att ttg ctg ctg gat ctt cct cca 787
Asp Val Phe Trp Gly Asp Leu Asp Ile Leu Leu Leu Asp Lau Pro Pro
215 220 225
gga act ggt gac atc gcc atc acc gtt gcc caa ttg atc ccg aat gct 835
Gly Thr Gly Asp Ile Ala Ile Thr Val Ala Gln Leu Ile Pro Asn Ala
230 235 240 245
gag ttg ctc att gtg acc act cct cag gct gcc gca gct gag gtt gcc 883
Glu Leu Leu Ile Val Thr Thr Pro Gln Ala Ala Ala Ala Glu Val Ala
250 255 260
gag cga gca gga acg atc tct gtg cag acc aac cag aag gtt gct ggc 931
Glu Arg Ala Gly Thr Ile Ser Val Gln Thr Asn Gln Lys Val Ala Gly
265 270 275
gtg att gaa aac atg tct gcc atg gtg ctt cct gat ggc acc acc atg 979
Val Ile Glu Asn Met Ser Ala Met Val Leu Pro Asp Gly Thr Thr Met
280 285 290
gat gtt ttc ggc acc ggc ggc ggt caa aag att gct gat cgt ctt acc 1027
Asp Val Phe Gly Thr Gly Gly Gly Gln Lys Ile Ala Asp Arg Leu Thr
295 300 305
gct gtg aca ggt gaa gag gtc aag gtt atc gga tct gtt cca ttg gat 1075
Ala Val Thr Gly Glu Glu Val Lys Val Ile Gly Ser Val Pro Leu Asp
310 315 320 325
ccg aac ctg cgt atc ggt ggc gat gtg gga aat cct att gcg att tct 1123
Pro Asn Leu Arg Ile Gly Gly Asp Val Gly Asn Pro Ile Ala Ile Ser
330 335 340
gaa cca cac tcc cca acc gct gca gcg atc aat gag atc gct gaa cac 1171
Glu Pro His Ser Pro Thr Ala Ala Ala Ile Asn Glu Ile Ala Glu His
345 350 355
cta gct cac cgc aag gta tcg ctg gtg ggt aaa acg ctt ggg ctt ggc 1219
Leu Ala His Arg Lys Val Ser Leu Val Gly Lys Thr Leu Gly Leu Gly
360 365 370
gtt aaa taaaagctaa ataatatcgg tccaagagac 1255
Val Lys
375
<210>52
<211>375
<212>PRT
<213>谷氨酸棒状杆菌
<400>52
Val Met Thr Gln Val Thr Glu Ser Ala Val Arg Ser Ala Leu Ser Arg
1 5 10 15
Val Glu Asp Pro Glu Ile Gly Lys Pro Ile Thr Glu Leu Gly Met Val
20 25 30
Lys Ser Val Ser Ile Asp Gly Ser Asp Val Gln Val Glu Val Tyr Lau
35 40 45
Thr Ile Ala Ala Cys Pro Met Lys Thr Thr Ile Val Thr Asn Thr Glu
50 55 60
Ala Ala Leu Lys Asp Ile Asp Gly Val Gly Gln Val His Val Thr Thr
65 70 75 80
Asp Val Met Ser Asp Glu Gln Arg Arg Ala Leu Arg Val Ser Leu Arg
85 90 95
Gly Glu Thr Ser Glu Pro Val Ile Pro Phe Ala Gln Pro Gly Ser Thr
100 105 110
Thr Arg Val Tyr Ala Val Ala Ser Gly Lys Gly Gly Val Gly Lys Ser
115 120 125
Ser Met Thr Val Asn Leu Ala Ala Ala Leu Ala Lys Arg Gly Leu Ser
130 135 140
Val Gly Ile Leu Asp Ala Asp Ile Tyr Gly His Ser Val Pro Gly Met
145 150 155 160
Leu Gly Ser Asp Gln Arg Pro His Gln Val Asp Asp Met Ile Met Pro
165 170 175
Pro Gln Ala His Gly Val Lys Met Ile Ser Ile Ala His Phe Thr Glu
180 185 190
Gly Asn Ala Pro Val Val Trp Arg Gly Pro Met Leu His Arg Ala Ile
195 200 205
Gln Gln Phe Leu Thr Asp Val Phe Trp Gly Asp Leu Asp Ile Leu Leu
210 215 220
Leu Asp Leu Pro Pro Gly Thr Gly Asp Ile Ala Ile Thr Val Ala Gln
225 230 235 240
Leu Ile Pro Asn Ala Glu Leu Leu Ile Val Thr Thr pro Gln Ala Ala
245 250 255
Ala Ala Glu Val Ala Glu Arg Ala Gly Thr Ile Ser Val Gln Thr Asn
260 265 270
Gln Lys Val Ala Gly Val Ile Glu Asn Met Ser Ala Met Val Leu Pro
275 280 285
Asp Gly Thr Thr Met Asp Val Phe Gly Thr Gly Gly Gly Gln Lys Ile
290 295 300
Ala Asp Arg Leu Thr Ala Val Thr Gly Glu Glu Val Lys Val Ile Gly
305 310 315 320
Ser Val Pro Leu Asp Pro Asn Leu Arg Ile Gly Gly Asp Val Gly Asn
325 330 335
Pro Ile Ala Ile Ser Glu Pro His Ser Pro Thr Ala Ala Ala Ile Asn
340 345 350
Glu Ile Ala Glu His Leu Ala His Arg Lys Val Ser Leu Val Gly Lys
355 360 365
Thr Leu Gly Leu Gly Val Lys
370 375
<210>53
<211>3595
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3565)
<223>RXA02082
<400>53
gctcgtatcg cagaccaggt catgtggaca cagttattga gcaatggggc gagccgcgtg 60
acgttgaagg ctttgtggag cgctagactt taaccccgtt atg tat ttg aaa tcg 115
Met Tyr Leu Lys Ser
1 5
ttg acg ctc aag ggg ttt aag tct ttc gcg tct gcg acg acc ctg aaa 163
Leu Thr Leu Lys Gly Phe Lys Ser Phe Ala Ser Ala Thr Thr Leu Lys
10 15 20
ttt gag cca ggc att tgt gcc gtg gtg ggt ccg aat ggt tca ggc aaa 211
Phe Glu Pro Gly Ile Cys Ala Val Val Gly Pro Asn Gly Ser Gly Lys
25 30 35
tcc aat gtg gtt gat gcg ctg gcc tgg gtg atg ggt gaa ggt tct gcg 259
Ser Asn Val Val Asp Ala Leu Ala Trp Val Met Gly Glu Gly Ser Ala
40 45 50
aag acc ttg cgt ggc ggc aaa atg gaa gat gtc att ttt gct ggc gcg 307
Lys Thr Leu Arg Gly Gly Lys Met Glu Asp Val Ile Phe Ala Gly Ala
55 60 65
ggc gat cgt aaa ccg ttg ggt cgc gca gaa gtc acg ctg acc att gat 355
Gly Asp Arg Lys Pro Leu Gly Arg Ala Glu Val Thr Leu Thr Ile Asp
70 75 80 85
aac tct gat ggc gca ctg ccc att gag tac acc gaa gtg tcg gtg acc 403
Asn Ser Asp Gly Ala Leu Pro Ile Glu Tyr Thr Glu Val Ser Val Thr
90 95 100
aga cgg atg ttc cgt gat ggt gca agt gaa tat gag atc aat ggg gcg 451
Arg Arg Met Phe Arg Asp Gly Ala Ser Glu Tyr Glu Ile Asn Gly Ala
105 110 115
aaa gct cga ttg atg gat atc cag gag ctg ttg tcg gat acc ggt att 499
Lys Ala Arg Leu Met Asp Ile Gln Glu Leu Leu Ser Asp Thr Gly Ile
120 125 130
ggc cgt gaa atg cac atc atg gtg ggg cag gga aag ctc gca gag att 547
Gly Arg Glu Met His Ile Met Val Gly Gln Gly Lys Leu Ala Glu Ile
135 140 145
ttg gag tcc cgc ccc gaa gag cgc cga gcg tat atc gaa gaa gct gcg 595
Leu Glu Ser Arg Pro Glu Glu Arg Arg Ala Tyr Ile Glu Glu Ala Ala
150 155 160 165
ggt gtg ctc aag cac cgg cgc agg aaa gaa aag gcg cag cgc aaa ctt 643
Gly Val Leu Lys His Arg Arg Arg Lys Glu Lys Ala Gln Arg Lys Leu
170 175 180
cag ggc atg cag gtc aat ctt gat cgt ttg cag gat ctg acc cat gag 691
Gln Gly Met Gln Val Asn Leu Asp Arg Leu Gln Asp Leu Thr His Glu
185 190 195
ttg gcc aag cag ctc aag ccg ttg gct agg cag gcg gaa gca gcg cag 739
Leu Ala Lys Gln Leu Lys Pro Leu Ala Arg Gln Ala Glu Ala Ala Gln
200 205 210
cgt gcg gcg acg gtg cag gct gat ttg cgt gat gcg cgt ttc cag att 787
Arg Ala Ala Thr Val Gln Ala Asp Leu Arg Asp Ala Arg Phe Gln Ile
215 220 225
gct ggc ttt gag atc gtg aag ctc tcg gaa aag ctg gaa acc tct act 835
Ala Gly Phe Glu Ile Val Lys Leu Ser Glu Lys Leu Glu Thr Ser Thr
230 235 240 245
gag cgc gag aaa atg att cgt gag cag gcg gaa gca gca caa gag cag 883
Glu Arg Glu Lys Met Ile Arg Glu Gln Ala Glu Ala Ala Gln Glu Gln
250 255 260
ctg gaa gaa gcc acc aca act cag atg gaa gtg gag atg gag ttg gcg 931
Leu Glu Glu Ala Thr Thr Thr Gln Met Glu Val Glu Met Glu Leu Ala
265 270 275
gag atc act ccg cag gct gaa gct gcg caa cag ttg tgg ttt gat ttg 979
Glu Ile Thr Pro Gln Ala Glu Ala Ala Gln Gln Leu Trp Phe Asp Leu
280 285 290
tct tcg ctg gct gag cgg gtg tcg gca acg atg cgt att gct gca gac 1027
Ser Ser Leu Ala Glu Arg Val Ser Ala Thr Met Arg Ile Ala Ala Asp
295 300 305
cgt gcg agt tca ggt gcc gcg gat gtg ccg tat gcg ggc cag gat cct 1075
Arg Ala Ser Ser Gly Ala Ala Asp Val Pro Tyr Ala Gly Gln Asp Pro
310 315 320 325
gat gag ttg ctt ggt cgg gcc gaa act gct gac aaa gaa tta gaa gaa 1123
Asp Glu Leu Leu Gly Arg Ala Glu Thr Ala Asp Lys Glu Leu Glu Glu
330 335 340
ctc gag atg gcc gtg gaa atg acc acc gag cgt ttg acc tcc att caa 1171
Leu Glu Met Ala Val Glu Met Thr Thr Glu Arg Leu Thr Ser Ile Gln
345 350 355
gag gaa gcc gag gat aag gcc gcg cag gct cgt gag gct gag cgt gaa 1219
Glu Glu Ala Glu Asp Lys Ala Ala Gln Ala Arg Glu Ala Glu Arg Glu
360 365 370
cac ttg gcg cag gtc agg gcg att tct gat cgt cgt gaa ggt gtt gtg 1267
His Leu Ala Gln Val Arg Ala Ile Ser Asp Arg Arg Glu Gly Val Val
375 380 385
cgc ctg ctt gca tct gag gaa tct ttg cgc acc cag cac acg tca gca 1315
Arg Leu Leu Ala Ser Glu Glu Ser Leu Arg Thr Gln His Thr Ser Ala
390 395 400 405
gag gag gaa gct gag cga ctc agt gag cag ctt gag gag ttc atc ggc 1363
Glu Glu Glu Ala Glu Arg Leu Ser Glu Gln Leu Glu Glu Phe Ile Gly
410 415 420
cgc att ttg gat gtg gaa cgt gaa cgt cgc ctc acc gat gag cgt aaa 1411
Arg Ile Leu Asp Val Glu Arg Glu Arg Arg Leu Thr Asp Glu Arg Lys
425 430 435
cag ggc gtt gac acg gat cgt gcg ccc ttg gaa gaa gcc ctc aaa cag 1459
Gln Gly Val Asp Thr Asp Arg Ala Pro Leu Glu Glu Ala Leu Lys Gln
440 445 450
gca aaa cat gaa gcc gaa gca gca gag act cgt ctt gag gag ctt cgt 1507
Ala Lys His Glu Ala Glu Ala Ala Glu Thr Arg Leu Glu Glu Leu Arg
455 460 465
act aag cgc agc gat ctg gaa aaa gaa gta tcc agg ttg cag tcg cgc 1555
Thr Lys Arg Ser Asp Leu Glu Lys Glu Val Ser Arg Leu Gln Ser Arg
470 475 480 485
att gag acg ctt aac caa aat agg cca cgt tcc gat gct gct gat gtg 1603
Ile Glu Thr Leu Asn Gln Asn Arg Pro Arg Ser Asp Ala Ala Asp Val
490 495 500
gtg gat tac ccg cag ctg gcc acg ttg att cga ccg caa cga aac gtc 1651
Val Asp Tyr Pro Gln Leu Ala Thr Leu Ile Arg Pro Gln Arg Asn Val
505 510 515
gat aag gct ctc gct gcc gcc ctg ggt gcg cat gcc gag gcg ctg gct 1699
Asp Lys Ala Leu Ala Ala Ala Leu Gly Ala His Ala Glu Ala Leu Ala
520 525 530
ggc gag gct gcg gaa ggg ctc gtc gag aag ctt atc gac gcc ggc gtt 1747
Gly Glu Ala Ala Glu Gly Leu Val Glu Lys Leu Ile Asp Ala Gly Val
535 540 545
gca cgc acc atc atc gtt gat ggc acg cag gct ggc ggc gca tgg cgc 1795
Ala Arg Thr Ile Ile Val Asp Gly Thr Gln Ala Gly Gly Ala Trp Arg
550 555 560 565
ctg gac gcg aac att ccg gcc ggg gcg agc tgg ctg ctc gac cat gtt 1843
Leu Asp Ala Asn Ile Pro Ala Gly Ala Ser Trp Leu Leu Asp His Val
570 575 580
gat ctg gat ccg gcg att gcc ggc ccg gta aac cgg ctg ctt gcc gac 1891
Asp Leu Asp Pro Ala Ile Ala Gly Pro Val Asn Arg Leu Leu Ala Asp
585 590 595
gtt gtg ctt gtc gac gac ccc tcc ctc ggc cgc caa gca atc gag gat 1939
Val Val Leu Val Asp Asp Pro Ser Leu Gly Arg Gln Ala Ile Glu Asp
600 605 610
gat ccc cgt ctg cgt gcc gtt gac cgc aat ggt gtg ctc atc ggc gct 1987
Asp Pro Arg Leu Arg Ala Val Asp Arg Asn Gly Val Leu Ile Gly Ala
615 620 625
ggg tgg att cag gtc ggc acc gaa acc tcg act gtg gaa atc aca gct 2035
Gly Trp Ile Gln Val Gly Thr Glu Thr Ser Thr Val Glu Ile Thr Ala
630 635 640 645
caa att gag gaa gca gaa gct caa ctt gct gcg gcc tct gcc gcc ttg 2083
Gln Ile Glu Glu Ala Glu Ala Gln Leu Ala Ala Ala Ser Ala Ala Leu
650 655 660
gac gac att gcc ggc act ttt gat ggc gcc ctc cac gct gcc gac aac 2131
Asp Asp Ile Ala Gly Thr Phe Asp Gly Ala Leu His Ala Ala Asp Asn
665 670 675
act cgc gtc gag gtg gct gcc cgc acc gca gcc ctg cgc gaa ctc gac 2179
Thr Arg Val Glu Val Ala Ala Arg Thr Ala Ala Leu Arg Glu Leu Asp
680 685 690
atg acc agg gat tcc atc acc cgc gat ctc gcg cgc ttg gac aaa caa 2227
Met Thr Arg Asp Ser Ile Thr Arg Asp Leu Ala Arg Leu Asp Lys Gln
695 700 705
cat gag gcc gcc gaa tcc gag cgc gtc cgc cat gtt gga cgc ctg cat 2275
His Glu Ala Ala Glu Ser Glu Arg Val Arg His Val Gly Arg Leu His
710 715 720 725
gct gcg gaa aca cgc cgt gaa gag ctg cgc gaa cag tta gaa gac atc 2323
Ala Ala Glu Thr Arg Arg Glu Glu Leu Arg Glu Gln Leu Glu Asp Ile
730 735 740
gtc gat cga ctc tcc cgc gtg gaa gac gaa gaa gac gct gac gaa ccc 2371
Val Asp Arg Leu Ser Arg Val Glu Asp Glu Glu Asp Ala Asp Glu Pro
745 750 755
tca acc acc gcc cgc gac caa gca aat gcc gag ctg caa caa atc cgc 2419
Ser Thr Thr Ala Arg Asp Gln Ala Asn Ala Glu Leu Gln Gln Ile Arg
760 765 770
gcc atg gaa atg gaa gca cgc ctt gcc caa cgc acc gcc gaa gag cgc 2467
Ala Met Glu Met Glu Ala Arg Leu Ala Gln Arg Thr Ala Glu Glu Arg
775 780 785
gcc ggg cag cag cgg ggc aag ggc gat agt ctg cga cgc cag gcc gag 2515
Ala Gly Gln Gln Arg Gly Lys Gly Asp Ser Leu Arg Arg Gln Ala Glu
790 795 800 805
cat gag cgc caa gcc aaa atc cgg cat gaa caa gcc atg gaa gcc cgt 2563
His Glu Arg Gln Ala Lys Ile Arg His Glu Gln Ala Met Glu Ala Arg
810 815 820
cgc agg cgc acc caa ttg gct gca gcc gtg caa aat ggc gca egc gat 2611
Arg Arg Arg Thr Gln Leu Ala Ala Ala Val Gln Asn Gly Ala Arg Asp
825 830 835
gtg gcc gag cgt gtc tca gct gcc ctt gcc caa gca gcc atc gaa aga 2659
Val Ala Glu Arg Val Ser Ala Ala Leu Ala Gln Ala Ala Ile Glu Arg
840 845 850
gat cag cac aac cgc gac aaa gcg ctg ctg acc tca cac tta gcg cgc 2707
Asp Gln His Asn Arg Asp Lys Ala Leu Leu Thr Ser His Leu Ala Arg
855 860 865
gcc aaa gat gct gtg agt gct gca cgc cag cac ctc aat cga ctc agc 2755
Ala Lys Asp Ala Val Ser Ala Ala Arg Gln His Leu Asn Arg Leu Ser
870 875 880 885
gac aac gcc cac tcc atg gaa ctt gcg cgc agc caa gct caa gtg cgc 2803
Asp Asn Ala His Ser Met Glu Leu Ala Arg Ser Gln Ala Gln Val Arg
890 895 900
atg gag gaa gcc gtt gcc aaa atc acc gag caa ctt ggc att ccg gtc 2851
Met Glu Glu Ala Val Ala Lys Ile Thr Glu Gln Leu Gly Ile Pro Val
905 910 915
gca gaa ttg ctg cgc gat tac acc cca gat gaa aac ttt gat gaa aag 2899
Ala Glu Leu Leu Arg Asp Tyr Thr Pro Asp Glu Asn Phe Asp Glu Lys
920 925 930
ttc caa cgg gca cgc ctc aaa caa gcc gaa aaa gac ctc gcc gca cta 2947
Phe Gln Arg Ala Arg Leu Lys Gln Ala Glu Lys Asp Leu Ala Ala Leu
935 940 945
ggc aaa gtc aac ccc ctg gcc ttg gaa gaa ttc aaa gcc ttg gaa gag 2995
Gly Lys Val Asn Pro Leu Ala Leu Glu Glu Phe Lys Ala Leu Glu Glu
950 955 960 965
cgc tac gag ttc ctc tcc acc caa ttg gcc gat gtg gaa caa gcc cgc 3043
Arg Tyr Glu Phe Leu Ser Thr Gln Leu Ala Asp Val Glu Gln Ala Arg
970 975 980
gcc gat ctg agc ggc gtg att gaa gaa gtc gat gcg aaa att ctg cag 3091
Ala Asp Leu Ser Gly Val Ile Glu Glu Val Asp Ala Lys Ile Leu Gln
985 990 995
ctt ttc acc gat gcc tgg aac gac gtg gaa gca gaa ttc cca cgc gtg 3139
Leu Phe Thr Asp Ala Trp Asn Asp Val Glu Ala Glu Phe Pro Arg Val
1000 1005 1010
ttc aac acc ttg ttc cca ggt ggc gaa ggc aaa ctg atc ctc acc gag 3187
Phe Asn Thr Leu Phe Pro Gly Gly Glu Gly Lys Leu Ile Leu Thr Glu
1015 1020 1025
ccc gat gat ttg ctg gca acc ggc atc gaa gtg gaa gcc cgc cca cca 3235
Pro Asp Asp Leu Leu Ala Thr Gly Ile Glu Val Glu Ala Arg Pro Pro
1030 1035 1040 1045
gga aaa cgc gtc aag cgc cta tcg ctg cta tcc ggt ggc gaa aaa tcc 3283
Gly Lys Arg Val Lys Arg Leu Ser Leu Leu Ser Gly Gly Glu Lys Ser
1050 1055 1060
ctc acc gca cta gcc atg ctc gtc gcg att ttc cgc gcc cga ccc agc 3331
Leu Thr Ala Leu Ala Met Leu Val Ala Ile Phe Arg Ala Arg Pro Ser
1065 1070 1075
cct ttc tac gtg atg gat gag gtg gaa gca gca ctc gat gat gtc aac 3379
Pro Phe Tyr Val Met Asp Glu Val Glu Ala Ala Leu Asp Asp Val Asn
1080 1085 1090
ctt cgc cga ctt atc gca ctg ttt gaa gag ttg cgc cgc gac tcc cag 3427
Leu Arg Arg Leu Ile Ala Leu Phe Glu Glu Leu Arg Arg Asp Ser Gln
1095 1100 1105
ctc att gtg atc acc cac caa aaa ccc acc atg gat gtg gct aac gta 3475
Leu Ile Val Ile Thr His Gln Lys Pro Thr Met Asp Val Ala Asn Val
1110 1115 1120 1125
ctc tac ggc gtc acc atg cgt ggc gat ggc gtt acc cgc gtg atc tcc 3523
Leu Tyr Gly Val Thr Met Arg Gly Asp Gly Val Thr Arg Val Ile Ser
1130 1135 1140
caa cgc atg gaa cct gct tct gca atg gaa act gtg gcg ggg 3565
Gln Arg Met Glu Pro Ala Ser Ala Met Glu Thr Val Ala Gly
1145 1150 1155
taaaacctgc gctgagcacg gatgatgttc 3595
<210>54
<211>1155
<212>PRT
<213>谷氨酸棒状杆菌
<400>54
Met Tyr Leu Lys Ser Leu Thr Leu Lys Gly Phe Lys Ser Phe Ala Ser
1 5 10 15
Ala Thr Thr Leu Lys Phe Glu Pro Gly Ile Cys Ala Val Val Gly Pro
20 25 30
Asn Gly Ser Gly Lys Ser Asn Val Val Asp Ala Leu Ala Trp Val Met
35 40 45
Gly Glu Gly Ser Ala Lys Thr Leu Arg Gly Gly Lys Met Glu Asp Val
50 55 60
Ile Phe Ala Gly Ala Gly Asp Arg Lys Pro Leu Gly Arg Ala Glu Val
65 70 75 80
Thr Leu Thr Ile Asp Asn Ser Asp Gly Ala Leu Pro Ile Glu Tyr Thr
85 90 95
Glu Val Ser Val Thr Arg Arg Met Phe Arg Asp Gly Ala Ser Glu Tyr
100 105 110
Glu Ile Asn Gly Ala Lys Ala Arg Leu Met Asp Ile Gln Glu Leu Leu
115 120 125
Ser Asp Thr Gly Ile Gly Arg Glu Met His Ile Met Val Gly Gln Gly
130 135 140
Lys Leu Ala Glu Ile Leu Glu Ser Arg Pro Glu Glu Arg Arg Ala Tyr
145 150 155 160
Ile Glu Glu Ala Ala Gly Val Leu Lys His Arg Arg Arg Lys Glu Lys
165 170 175
Ala Gln Arg Lys Leu Gln Gly Met Gln Val Asn Leu Asp Arg Leu Gln
180 185 190
Asp Leu Thr His Glu Leu Ala Lys Gln Leu Lys Pro Leu Ala Arg Gln
195 200 205
Ala Glu Ala Ala Gln Arg Ala Ala Thr Val Gln Ala Asp Leu Arg Asp
210 215 220
Ala Arg Phe Gln Ile Ala Gly Phe Glu Ile Val Lys Leu Ser Glu Lys
225 230 235 240
Leu Glu Thr Ser Thr Glu Arg Glu Lys Met Ile Arg Glu Gln Ala Glu
245 250 255
Ala Ala Gln Glu Gln Leu Glu Glu Ala Thr Thr Thr Gln Met Glu Val
260 265 270
Glu Met Glu Leu Ala Glu Ile Thr Pro Gln Ala Glu Ala Ala Gln Gln
275 280 285
Leu Trp Phe Asp Leu Ser Ser Leu Ala Glu Arg Val Ser Ala Thr Met
290 295 300
Arg Ile Ala Ala Asp Arg Ala Ser Ser Gly Ala Ala Asp Val Pro Tyr
305 310 315 320
Ala Gly Gln Asp Pro Asp Glu Leu Leu Gly Arg Ala Glu Thr Ala Asp
325 330 335
Lys Glu Leu Glu Glu Leu Glu Met Ala Val Glu Met Thr Thr Glu Arg
340 345 350
Leu Thr Ser Ile Gln Glu Glu Ala Glu Asp Lys Ala Ala Gln Ala Arg
355 360 365
Glu Ala Glu Arg Glu His Leu Ala Gln Val Arg Ala Ile Ser Asp Arg
370 375 380
Arg Glu Gly Val Val Arg Leu Leu Ala Ser Glu Glu Ser Leu Arg Thr
385 390 395 400
Gln His Thr Ser Ala Glu Glu Glu Ala Glu Arg Leu Ser Glu Gln Leu
405 410 415
Glu Glu Phe Ile Gly Arg Ile Leu Asp Val Glu Arg Glu Arg Arg Leu
420 425 430
Thr Asp Glu Arg Lys Gln Gly Val Asp Thr Asp Arg Ala Pro Leu Glu
435 440 445
Glu Ala Leu Lys Gln Ala Lys His Glu Ala Glu Ala Ala Glu Thr Arg
450 455 460
Leu Glu Glu Leu Arg Thr Lys Arg Ser Asp Leu Glu Lys Glu Val Ser
465 470 475 480
Arg Leu Gln Ser Arg Ile Glu Thr Leu Asn Gln Asn Arg Pro Arg Ser
485 490 495
Asp Ala Ala Asp Val Val Asp Tyr Pro Gln Leu Ala Thr Leu Ile Arg
500 505 510
Pro Gln Arg Asn Val Asp Lys Ala Leu Ala Ala Ala Leu Gly Ala His
515 520 525
Ala Glu Ala Leu Ala Gly Glu Ala Ala Glu Gly Leu Val Glu Lys Leu
530 535 540
Ile Asp Ala Gly Val Ala Arg Thr Ile Ile Val Asp Gly Thr Gln Ala
545 550 555 560
Gly Gly Ala Trp Arg Leu Asp Ala Asn Ile Pro Ala Gly Ala Ser Trp
565 570 575
Leu Leu Asp His Val Asp Leu Asp Pro Ala Ile Ala Gly Pro Val Asn
580 585 590
Arg Leu Leu Ala Asp Val Val Leu Val Asp Asp Pro Ser Leu Gly Arg
595 600 605
Gln Ala Ile Glu Asp Asp Pro Arg Leu Arg Ala Val Asp Arg Asn Gly
610 615 620
Val Leu Ile Gly Ala Gly Trp Ile Gln Val Gly Thr Glu Thr Ser Thr
625 630 635 640
Val Glu Ile Thr Ala Gln Ile Glu Glu Ala Glu Ala Gln Leu Ala Ala
645 650 655
Ala Ser Ala Ala Leu Asp Asp Ile Ala Gly Thr Phe Asp Gly Ala Leu
660 665 670
His Ala Ala Asp Asn Thr Arg Val Glu Val Ala Ala Arg Thr Ala Ala
675 680 685
Leu Arg Glu Leu Asp Met Thr Arg Asp Ser Ile Thr Arg Asp Leu Ala
690 695 700
Arg Leu Asp Lys Gln His Glu Ala Ala Glu Ser Glu Arg Val Arg His
705 710 715 720
Val Gly Arg Leu His Ala Ala Glu Thr Arg Arg Glu Glu Leu Arg Glu
725 730 735
Gln Leu Glu Asp Ile Val Asp Arg Leu Ser Arg Val Glu Asp Glu Glu
740 745 750
Asp Ala Asp Glu Pro Ser Thr Thr Ala Arg Asp Gln Ala Asn Ala Glu
755 760 765
Leu Gln Gln Ile Arg Ala Met Glu Met Glu Ala Arg Leu Ala Gln Arg
770 775 780
Thr Ala Glu Glu Arg Ala Gly Gln Gln Arg Gly Lys Gly Asp Ser Leu
785 790 795 800
Arg Arg Gln Ala Glu His Glu Arg Gln Ala Lys Ile Arg His Glu Gln
805 810 815
Ala Met Glu Ala Arg Arg Arg Arg Thr Gln Leu Ala Ala Ala Val Gln
820 825 830
Asn Gly Ala Arg Asp Val Ala Glu Arg Val Ser Ala Ala Leu Ala Gln
835 840 845
Ala Ala Ile Glu Arg Asp Gln His Asn Arg Asp Lys Ala Leu Leu Thr
850 855 860
Ser His Leu Ala Arg Ala Lys Asp Ala Val Ser Ala Ala Arg Gln His
865 870 875 880
Leu Asn Arg Leu Ser Asp Asn Ala His Ser Met Glu Leu Ala Arg Ser
885 890 895
Gln Ala Gln Val Arg Met Glu Glu Ala Val Ala Lys Ile Thr Glu Gln
900 905 910
Leu Gly Ile Pro Val Ala Glu Leu Leu Arg Asp Tyr Thr Pro Asp Glu
915 920 925
Asn Phe Asp Glu Lys Phe Gln Arg Ala Arg Leu Lys Gln Ala Glu Lys
930 935 940
Asp Leu Ala Ala Leu Gly Lys Val Asn Pro Leu Ala Leu Glu Glu Phe
945 950 955 960
Lys Ala Leu Glu Glu Arg Tyr Glu Phe Leu Ser Thr Gln Leu Ala Asp
965 970 975
Val Glu Gln Ala Arg Ala Asp Leu Ser Gly Val Ile Glu Glu Val Asp
980 985 990
Ala Lys Ile Leu Gln Leu Phe Thr Asp Ala Trp Asn Asp Val Glu Ala
995 1000 1005
Glu Phe Pro Arg Val Phe Asn Thr Leu Phe Pro Gly Gly Glu Gly Lys
1010 1015 1020
Leu Ile Leu Thr Glu Pro Asp Asp Leu Leu Ala Thr Gly Ile Glu Val
1025 1030 1035 1040
Glu Ala Arg Pro Pro Gly Lys Arg Val Lys Arg Leu Ser Leu Leu Ser
1045 1050 1055
Gly Gly Glu Lys Ser Leu Thr Ala Leu Ala Met Leu Val Ala Ile Phe
1060 1065 1070
Arg Ala Arg Pro Ser Pro Phe Tyr Val Met Asp Glu Val Glu Ala Ala
1075 1080 1085
Leu Asp Asp Val Asn Leu Arg Arg Leu Ile Ala Leu Phe Glu Glu Leu
1090 1095 1100
Arg Arg Asp Ser Gln Leu Ile Val Ile Thr His Gln Lys Pro Thr Met
1105 1110 1115 1120
Asp Val Ala Asn Val Leu Tyr Gly Val Thr Met Arg Gly Asp Gly Val
1125 1130 1135
Thr Arg Val Ile Ser Gln Arg Met Glu Pro Ala Ser Ala Met Glu Thr
1140 1145 1150
Val Ala Gly
1155
<210>55
<211>1747
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1717)
<223>RXA02145
<400>55
cactgccaca gctgccaatt accgttgatg aagagggcta cctcatcgcc gctggtaact 60
tcattgagcc actcggccct gcattctggg agcgtaagtc atg agt cta gct acc 115
Met Ser Leu Ala Thr
1 5
gtg gga aac aat ctt gat tcc cgt tac acc atg gcg tcg ggt atc cgt 163
Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met Ala Ser Gly Ile Arg
10 15 20
cgc cag atc aac aag gtc ttc cea act cac tgg tcc ttc atg ctc ggc 211
Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp Ser Phe Met Leu Gly
25 30 35
gag att gcg ctt tac agc ttc atc gtc ttg ctg ctg act ggt gtc tac 259
Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu Leu Thr Gly Val Tyr
40 45 50
ctg acc ctg ttc ttc gac cca tca atc acc aag gtc att tat gac ggc 307
Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys Val Ile Tyr Asp Gly
55 60 65
ggc tac ctc cca ctg aac ggt gtg gag atg tcc cgt gca tac gca act 355
Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser Arg Ala Tyr Ala Thr
70 75 80 85
gcg ttg gat att tcc ttc gag gtt cgc ggt ggt ctg ttc atc cgc cag 403
Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly Leu Phe Ile Arg Gln
90 95 100
atg cac cac tgg gca gcc ctg ctg ttc gtt gta tcc atg ctg gtt cac 451
Met His His Trp Ala Ala Leu Leu Phe Val Val Ser Met Leu Val His
105 110 115
atg ctc cgt att ttc ttc acc ggt gcg ttc cgt cgc cca cgt gaa gca 499
Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg Arg Pro Arg Glu Ala
120 125 130
aac tgg atc atc ggt gtt gtt ctg atc atc ctg ggt atg gct gaa ggc 547
Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu Gly Met Ala Glu Gly
135 140 145
ttc atg ggt tac tcc ctg cct gat gac ctg ctc tct ggt gtt ggt ctt 595
Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu Ser Gly Val Gly Leu
150 155 160 165
cga atc atg tcc gcc atc atc gtt ggt ctt ccg atc ata ggt acc tgg 643
Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro Ile Ile Gly Thr Trp
170 175 180
atg cac tgg ctg atc ttc ggt gga gac ttc cca tcc gat ctg atg ctg 691
Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro Ser Asp Leu Met Leu
185 190 195
gac cgc ttc tac atc gca cac gtt cta atc atc cca gct atc ctg ctt 739
Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile Pro Ala Ile Leu Leu
200 205 210
ggc ttg atc gca gct cac ctg gca ctt gtt tgg tac cag aag cac acc 787
Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp Tyr Gln Lys His Thr
215 220 225
cag ttc cca ggc gct ggc cgc act gag aac aac gtg atc ggt atc cga 835
Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn Val Ile Gly Ile Arg
230 235 240 245
atc atg cct ctg ttc gca gtt aag gct gtt gct ttc ggc ctc atc gtc 883
Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala Phe Gly Leu Ile Val
250 255 260
ttc ggt ttc ctc gca ctg ctt gct ggt gtc acc acc att aac gca att 931
Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr Thr Ile Asn Ala Ile
265 270 275
tgg aat ctt gga ccg tac aac cct tca cag gtg tct gct ggt tcc cag 979
Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val Ser Ala Gly Ser Gln
280 285 290
cct gac gtt tac atg ctg tgg aca gat ggt gct gct cgt gtc atg ccg 1027
Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala Ala Arg Val Met Pro
295 300 305
gca tgg gag ctc tac ctc ggt aac tac act att cca gca gtc ttc tgg 1075
Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile Pro Ala Val Phe Trp
310 315 320 325
gtt gct gtg atg ctg ggt atc ctc gtg gtt ctg ctt gtg act tac cca 1123
Val Ala Val Met Leu Gly Ile Leu Val Val Leu Leu Val Thr Tyr Pro
330 335 340
ttc att gag cgt aag ttc acc ggc gac gat gca cac cac aac ttg ctg 1171
Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala His His Asn Leu Leu
345 350 355
cag cgt cct cgc gat gtt cca gtc cgc acc tca ctc ggt gtc atg gcg 1219
Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser Leu Gly Val Met Ala
360 365 370
ctt gtc ttc tac atc ctg ctt acc gtt tct ggt ggt aac gat gtt tac 1267
Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly Gly Asn Asp Val Tyr
375 380 385
gca atg cag ttc cat gtt tca ctg aac gcg atg acc tgg atc ggt cgt 1315
Ala Met Gln Phe His Val Ser Leu Asn Ala Met Thr Trp Ile Gly Arg
390 395 400 405
atc ggc ctc atc gtt gga cca gct att gca tac ttc atc act tac cga 1363
Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr Phe Ile Thr Tyr Arg
410 415 420
ctg tgc atc ggc ttg cag cgc tct gac cgc gag gtc ctg gag cac ggc 1411
Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu Val Leu Glu His Gly
425 430 435
atc gag acc ggt atc atc aag cag atg cca aat ggt gcc ttc att gaa 1459
Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn Gly Ala Phe Ile Glu
440 445 450
gtt cac cag cca ctt ggc cca gtt gat gac cat ggt cac cca atc cca 1507
Val His Gln Pro Leu Gly Pro Val Asp Asp His Gly His Pro Ile Pro
455 460 465
ctg cca tac gct ggc gct gcg gtt cca aag cag atg aac cag ctt ggt 1555
Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln Met Asn Gln Leu Gly
470 475 480 485
tac gct gag gtt gaa acc cgc ggt gga ttc ttc gga cct gat cca gaa 1603
Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe Gly Pro Asp Pro Glu
490 495 500
gac atc cgt gcg aag gct aag gaa att gag cac gca aac cac att gag 1651
Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His Ala Asn His Ile Glu
505 510 515
gaa gcg aac act ctt cgt gca ctc aac gag gca aac att gag cgt gac 1699
Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala Asn Ile Glu Arg Asp
520 525 530
aag aat gag ggc aag aac tagtttctag gacttcatct ctgaaactcc 1747
Lys Asn Glu Gly Lys Asn
535
<210>56
<211>539
<212>PRT
<213>谷氨酸棒状杆菌
<400>56
Met Ser Leu Ala Thr Val Gly Asn Asn Leu Asp Ser Arg Tyr Thr Met
1 5 10 15
Ala Ser Gly Ile Arg Arg Gln Ile Asn Lys Val Phe Pro Thr His Trp
20 25 30
Ser Phe Met Leu Gly Glu Ile Ala Leu Tyr Ser Phe Ile Val Leu Leu
35 40 45
Leu Thr Gly Val Tyr Leu Thr Leu Phe Phe Asp Pro Ser Ile Thr Lys
50 55 60
Val Ile Tyr Asp Gly Gly Tyr Leu Pro Leu Asn Gly Val Glu Met Ser
65 70 75 80
Arg Ala Tyr Ala Thr Ala Leu Asp Ile Ser Phe Glu Val Arg Gly Gly
85 90 95
Leu Phe Ile Arg Gln Met His His Trp Ala Ala Leu Leu Phe Val Val
100 105 110
Ser Met Leu Val His Met Leu Arg Ile Phe Phe Thr Gly Ala Phe Arg
115 120 125
Arg Pro Arg Glu Ala Asn Trp Ile Ile Gly Val Val Leu Ile Ile Leu
130 135 140
Gly Met Ala Glu Gly Phe Met Gly Tyr Ser Leu Pro Asp Asp Leu Leu
145 150 155 160
Ser Gly Val Gly Leu Arg Ile Met Ser Ala Ile Ile Val Gly Leu Pro
165 170 175
Ile Ile Gly Thr Trp Met His Trp Leu Ile Phe Gly Gly Asp Phe Pro
180 185 190
Ser Asp Leu Met Leu Asp Arg Phe Tyr Ile Ala His Val Leu Ile Ile
195 200 205
Pro Ala Ile Leu Leu Gly Leu Ile Ala Ala His Leu Ala Leu Val Trp
210 215 220
Tyr Gln Lys His Thr Gln Phe Pro Gly Ala Gly Arg Thr Glu Asn Asn
225 230 235 240
Val Ile Gly Ile Arg Ile Met Pro Leu Phe Ala Val Lys Ala Val Ala
245 250 255
Phe Gly Leu Ile Val Phe Gly Phe Leu Ala Leu Leu Ala Gly Val Thr
260 265 270
Thr Ile Asn Ala Ile Trp Asn Leu Gly Pro Tyr Asn Pro Ser Gln Val
275 280 285
Ser Ala Gly Ser Gln Pro Asp Val Tyr Met Leu Trp Thr Asp Gly Ala
290 295 300
Ala Arg Val Met Pro Ala Trp Glu Leu Tyr Leu Gly Asn Tyr Thr Ile
305 310 315 320
Pro Ala Val Phe Trp Val Ala Val Met Leu Gly Ile Leu Val Val Leu
325 330 335
Leu Val Thr Tyr Pro Phe Ile Glu Arg Lys Phe Thr Gly Asp Asp Ala
340 345 350
His His Asn Leu Leu Gln Arg Pro Arg Asp Val Pro Val Arg Thr Ser
355 360 365
Leu Gly Val Met Ala Leu Val Phe Tyr Ile Leu Leu Thr Val Ser Gly
370 375 380
Gly Asn Asp Val Tyr Ala Met Gln Phe His Val Ser Leu Asn Ala Met
385 390 395 400
Thr Trp Ile Gly Arg Ile Gly Leu Ile Val Gly Pro Ala Ile Ala Tyr
405 410 415
Phe Ile Thr Tyr Arg Leu Cys Ile Gly Leu Gln Arg Ser Asp Arg Glu
420 425 430
Val Leu Glu His Gly Ile Glu Thr Gly Ile Ile Lys Gln Met Pro Asn
435 440 445
Gly Ala Phe Ile Glu Val His Gln Pro Leu Gly Pro Val Asp Asp His
450 455 460
Gly His Pro Ile Pro Leu Pro Tyr Ala Gly Ala Ala Val Pro Lys Gln
465 470 475 480
Met Asn Gln Leu Gly Tyr Ala Glu Val Glu Thr Arg Gly Gly Phe Phe
485 490 495
Gly Pro Asp Pro Glu Asp Ile Arg Ala Lys Ala Lys Glu Ile Glu His
500 505 510
Ala Asn His Ile Glu Glu Ala Asn Thr Leu Arg Ala Leu Asn Glu Ala
515 520 525
Asn Ile Glu Arg Asp Lys Asn Glu Gly Lys Asn
530 535
<210>57
<211>2557
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2527)
<223>RXA02293
<400>57
gtggggatat ccgcttcgcc acgaaggaaa aatttacttt ccacaacccg ctagaaaaca 60
catcatcgcc ttaggtttct ctgtagaagt taggaaaaag atg tgt tca ctc agc 115
Met Cys Ser Leu Ser
1 5
aaa ttc tta atg tac agg caa ttg atg agt tca aga att ggc aat ttt 163
Lys Phe Leu Met Tyr Arg Gln Leu Met Ser Ser Arg Ile Gly Asn Phe
10 15 20
ctt ata aat cgc att tcc acg ggc ctg ccc gta gaa aat att atc cct 211
Leu Ile Asn Arg Ile Ser Thr Gly Leu Pro Val Glu Asn Ile Ile Pro
25 30 35
cac ctg caa gaa gct ttt tcg gcc gga cct aaa aac ctt gtc att cag 259
His Leu Gln Glu Ala Phe Ser Ala Gly Pro Lys Asn Leu Val Ile Gln
40 45 50
gcc cct ccc ggc aca gga aaa acg aca ctt ctc ccc cca tta gta gct 307
Ala Pro Pro Gly Thr Gly Lys Thr Thr Leu Leu Pro Pro Leu Val Ala
55 60 65
aac atc tta tgt aat gaa gga gcg gga aat gcc acc cca acc aag gtt 355
Asn Ile Leu Cys Asn Glu Gly Ala Gly Asn Ala Thr Pro Thr Lys Val
70 75 80 85
tta gta acg gca cct cgt cgt gtg gcc gtc cga gca gca gct cgc aga 403
Leu Val Thr Ala Pro Arg Arg Val Ala Val Arg Ala Ala Ala Arg Arg
90 95 100
ctc gcc caa cta gat gat agc cag ctc gga aca aaa gtg gga ttc agc 451
Leu Ala Gln Leu Asp Asp Ser Gln Leu Gly Thr Lys Val Gly Phe Ser
105 110 115
gtg cgt ggt gaa cat att tca ggc tcc cac gtg caa ttt atg acc ccc 499
Val Arg Gly Glu His Ile Ser Gly Ser His Val Gln Phe Met Thr Pro
120 125 130
ggt gtg tta atc cgc cag ctc tta aac aac cca gaa tta ccc ggc atc 547
Gly Val Leu Ile Arg Gln Leu Leu Asn Asn Pro Glu Leu Pro Gly Ile
135 140 145
ggc gct gtg att atc gat gaa gtc cac gaa cgt caa ctc gat tcc gat 595
Gly Ala Val Ile Ile Asp Glu Val His Glu Arg Gln Leu Asp Ser Asp
150 155 160 165
cta ctg cta gga atg ctt gct gaa ctc agt caa ctg cgc gac gat ttc 643
Leu Leu Leu Gly Met Leu Ala Glu Leu Ser Gln Leu Arg Asp Asp Phe
170 175 180
tcc ctc atc gcc atg tcc gca acc tta gat tca gat aaa ttc gca aac 691
Ser Leu Ile Ala Met Ser Ala Thr Leu Asp Ser Asp Lys Phe Ala Asn
185 190 195
tta tta gac gct cag gtc ctt agt gtt gaa gca ccg att ttc ccc tta 739
Leu Leu Asp Ala Gln Val Leu Ser Val Glu Ala Pro Ile Phe Pro Leu
200 205 210
gac att tct tat gct cca gcc cgc gcg cct cgc ctc aat gca aaa ggt 787
Asp Ile Ser Tyr Ala Pro Ala Arg Ala Pro Arg Leu Asn Ala Lys Gly
215 220 225
gtc gac tgg gat ttc ctc gac cac atg gcg cag aaa act cac gat gcg 835
Val Asp Trp Asp Phe Leu Asp His Met Ala Gln Lys Thr His Asp Ala
230 235 240 245
gtg aca cat tca gag cac tct gca ctc att ttc gtc cca ggt gtg cgt 883
Val Thr His Ser Glu His Ser Ala Leu Ile Phe Val Pro Gly Val Arg
250 255 260
gaa att gat cga gta atg age acg ctc aaa tcg ctc ggc cat aat aat 931
Glu Ile Asp Arg Val Met Ser Thr Leu Lys Ser Leu Gly His Asn Asn
265 270 275
gtt ttc cca ctt cat ggc caa ctc agc ccg acc gaa caa gac cgc gcc 979
Val Phe Pro Leu His Gly Gln Leu Ser Pro Thr Glu Gln Asp Arg Ala
280 285 290
ctc gca ccg tca caa cag cag cgc atc att gtc tcc act ccc gtt gcg 1027
Leu Ala pro Ser Gln Gln Gln Arg Ile Ile Val Ser Thr Pro Val Ala
295 300 305
gaa agc tcc cta act gtg cct gga gtt cgc atc gtg gtg gat tcc ggc 1075
Glu Ser Ser Leu Thr Val Pro Gly Val Arg Ile Val Val Asp Ser Gly
310 315 320 325
ctc tca cga agc ccc aaa cga gac tct gcc cgc ggc atg aca gga ctg 1123
Leu Ser Arg Ser Pro Lys Arg Asp Ser Ala Arg Gly Met Thr Gly Leu
330 335 340
atc acc agc agt tgc gcg cag gct tca gca ggc caa cgc gca ggt cgc 1171
Ile Thr Ser Ser Cys Ala Gln Ala Ser Ala Gly Gln Arg Ala Gly Arg
345 350 355
gct ggc cgt gaa gga cca gga caa ata atc aga tgc tat tcc gaa gaa 1219
Ala Gly Arg Glu Gly Pro Gly Gln Ile Ile Arg Cys Tyr Ser Glu Glu
360 365 370
gac ttc tcc cac ttc cca cga ttt gtg aca cca gaa atc agc tcc gct 1267
Asp Phe Ser His Phe Pro Arg Phe Val Thr Pro Glu Ile Ser Ser Ala
375 380 385
gac ctc acc caa gca gcc ctc tgg ctc gcc caa tgg gga acg tca cct 1315
Asp Leu Thr Gln Ala Ala Leu Trp Leu Ala Gln Trp Gly Thr Ser Pro
390 395 400 405
gcg gat tta ccg ctt ctt gat caa ccc cct cac gca gcc tgg acg gct 1363
Ala Asp Leu Pro Leu Leu Asp Gln Pro Pro His Ala Ala Trp Thr Ala
410 415 420
gca caa cag atc ctg cgc ctc atc ggc gcg ctt gaa ggt gac gct atc 1411
Ala Gln Gln Ile Leu Arg Leu Ile Gly Ala Leu Glu Gly Asp Ala Ile
425 430 435
acg agc ctg gga cat cgc tta tcg acg ctt ccc ctc tgc cct caa ctc 1459
Thr Ser Leu Gly His Arg Leu Ser Thr Leu Pro Leu Cys Pro Gln Leu
440 445 450
agc gct tct ctc ctc cgc ttc ggt gaa caa tcc gca aaa atc ttg gcg 1507
Ser Ala Ser Leu Leu Arg Phe Gly Glu Gln Ser Ala Lys Ile Leu Ala
455 460 465
gtg gtg tcc gaa aac ccg cag ggg gac gtc gaa aag caa caa ccc gac 1555
Val Val Ser Glu Asn Pro Gln Gly Asp Val Glu Lys Gln Gln Pro Asp
470 475 480 485
aag cgt gaa gtg gaa cgc ctt cgc cgt ttg gca cct gcg tcg gtt gga 1603
Lys Arg Glu Val Glu Arg Leu Arg Arg Leu Ala Pro Ala Ser Val Gly
490 495 500
aaa gcg agt gcg ggg caa atc gta ggc gct gca ttc ccg cag ctc atc 1651
Lys Ala Ser Ala Gly Gln Ile Val Gly Ala Ala Phe Pro Gln Leu Ile
505 510 515
ggc cga aaa ata gac aat gga gaa tac ctt tta gcc agt gga acc cgc 1699
Gly Arg Lys Ile Asp Asn Gly Glu Tyr Leu Leu Ala Ser Gly Thr Arg
520 525 530
gca cgc ctc atg gat tca gat ctc aaa gat gcc gaa tgg atc tcc gtt 1747
Ala Arg Leu Met Asp Ser Asp Leu Lys Asp Ala Glu Trp Ile Ser Val
535 540 545
gct gca att aac cgc tca caa aat tct gcc atc atc cgc gcc gcc gcc 1795
Ala Ala Ile Asn Arg Ser Gln Asn Ser Ala Ile Ile Arg Ala Ala Ala
550 555 560 565
cgc att tea gag gac gat gcc ata gac atc atc gga gtc gtg gaa gaa 1843
Arg Ile Ser Glu Asp Asp Ala Ile Asp Ile Ile Gly Val Val Glu Glu
570 575 580
acc cgc gcg att ttt gtc aac gga aaa gtc caa gcc cgg aag gtc aaa 1891
Thr Arg Ala Ile Phe Val Asn Gly Lys Val Gln Ala Arg Lys Val Lys
585 590 595
gca gca ggc gcg atc gaa tta agt tcc act ccg aca aaa cca aca ccc 1939
Ala Ala Gly Ala Ile Glu Leu Ser Ser Thr Pro Thr Lys Pro Thr Pro
600 605 610
gcc gaa gcc tcg gaa aca atc gcc aca gcc ctt gcc aaa ggc gga atc 1987
Ala Glu Ala Ser Glu Thr Ile Ala Thr Ala Leu Ala Lys Gly Gly Ile
615 620 625
gac cta ttc cac ttc tcc gac aaa gcg gca tcc ttg cgt gac cga ctg 2035
Asp Leu Phe His Phe Ser Asp Lys Ala Ala Ser Leu Arg Asp Arg Leu
630 635 640 645
aaa ttc atc cac gaa cac cgt ggc gaa ccc tgg cca gat att gaa act 2083
Lys Phe Ile His Glu His Arg Gly Glu Pro Trp Pro Asp Ile Glu Thr
650 655 660
gcg gac ccg cac ctg tgg tta tct cca gaa att gag gcg ctc agc cac 2131
Ala Asp Pro His Leu Trp Leu Ser Pro Glu Ile Glu Ala Leu Ser His
665 670 675
gga aca cga cta aac aat atc gat atg tat ccc gca ctc cag cga ctt 2179
Gly Thr Arg Leu Asn Asn Ile Asp Met Tyr Pro Ala Leu Gln Arg Leu
680 685 690
ctc ccc tgg cct gaa gca acc aac ttt gaa gaa ttc gct cct tcc cac 2227
Leu Pro Trp Pro Glu Ala Thr Asn Phe Glu Glu Phe Ala Pro Ser His
695 700 705
cta tcc gta ccc agc ggg aat cag cac cga cta gat tat tca tca ggc 2275
Leu Ser Val Pro Ser Gly Asn Gln His Arg Leu Asp Tyr Ser Ser Gly
710 715 720 725
agg cca gtt ata cga gtg aaa tta caa gaa tgc ttc gga tta gaa gaa 2323
Arg Pro Val Ile Arg Val Lys Leu Gln Glu Cys Phe Gly Leu Glu Glu
730 735 740
tcc ccg cag ctc tgc gga atc ccg gtg caa ttt cat ctc ctg tca cct 2371
Ser Pro Gln Leu Cys Gly Ile Pro Val Gln Phe His Leu Leu Ser Pro
745 750 755
gcg gga aga cct ctt gca gtt acc gat gac ctg cgt agt ttc tgg tct 2419
Ala Gly Arg Pro Leu Ala Val Thr Asp Asp Leu Arg Ser Phe Trp Ser
760 765 770
ggc ccg tac agc caa gtc cgt gca gaa atg cgg ggc cgg tac ccc aaa 2467
Gly Pro Tyr Ser Gln Val Arg Ala Glu Met Arg Gly Arg Tyr Pro Lys
775 780 785
cac ccg tgg ccg gag gat cca tgg acc gct cct gcc act gcg cgc acc 2515
His Pro Trp Pro Glu Asp Pro Trp Thr Ala Pro Ala Thr Ala Arg Thr
790 795 800 805
aag aat cgc atg tagttttggc acgacttgat gtgagccgat 2557
Lys Asn Arg Met
<210>58
<211>809
<212>PRT
<213>谷氨酸棒状杆菌
<400>58
Met Cys Ser Leu Ser Lys Phe Leu Met Tyr Arg Gln Leu Met Ser Ser
1 5 10 15
Arg Ile Gly Asn Phe Leu Ile Asn Arg Ile Ser Thr Gly Leu Pro Val
20 25 30
Glu Asn Ile Ile Pro His Leu Gln Glu Ala Phe Ser Ala Gly Pro Lys
35 40 45
Asn Leu Val Ile Gln Ala Pro Pro Gly Thr Gly Lys Thr Thr Leu Leu
50 55 60
Pro Pro Leu Val Ala Asn Ile Leu Cys Asn Glu Gly Ala Gly Asn Ala
65 70 75 80
Thr Pro Thr Lys Val Leu Val Thr Ala Pro Arg Arg Val Ala Val Arg
85 90 95
Ala Ala Ala Arg Arg Leu Ala Gln Leu Asp Asp Ser Gln Leu Gly Thr
100 105 110
Lys Val Gly Phe Ser Val Arg Gly Glu His Ile Ser Gly Ser His Val
115 120 125
Gln Phe Met Thr Pro Gly Val Leu Ile Arg Gln Leu Leu Asn Asn Pro
130 135 140
Glu Leu Pro Gly Ile Gly Ala Val Ile Ile Asp Glu Val His Glu Arg
145 150 155 160
Gln Leu Asp Ser Asp Leu Leu Leu Gly Met Leu Ala Glu Leu Ser Gln
165 170 175
Leu Arg Asp Asp Phe Ser Leu Ile Ala Met Ser Ala Thr Leu Asp Ser
180 185 190
Asp Lys Phe Ala Asn Leu Leu Asp Ala Gln Val Leu Ser Val Glu Ala
195 200 205
Pro Ile Phe Pro Leu Asp Ile Ser Tyr Ala Pro Ala Arg Ala Pro Arg
210 215 220
Leu Asn Ala Lys Gly Val Asp Trp Asp Phe Leu Asp His Met Ala Gln
225 230 235 240
Lys Thr His Asp Ala Val Thr His Ser Glu His Ser Ala Leu Ile Phe
245 250 255
Val Pro Gly Val Arg Glu Ile Asp Arg Val Met Ser Thr Leu Lys Ser
260 265 270
Leu Gly His Asn Asn Val Phe Pro Leu His Gly Gln Leu Ser Pro Thr
275 280 285
Glu Gln Asp Arg Ala Leu Ala Pro Ser Gln Gln Gln Arg Ile Ile Val
290 295 300
Ser Thr Pro Val Ala Glu Ser Ser Leu Thr Val Pro Gly Val Arg Ile
305 310 315 320
Val Val Asp Ser Gly Leu Ser Arg Ser Pro Lys Arg Asp Ser Ala Arg
325 330 335
Gly Met Thr Gly Leu Ile Thr Ser Ser Cys Ala Gln Ala Ser Ala Gly
340 345 350
Gln Arg Ala Gly Arg Ala Gly Arg Glu Gly Pro Gly Gln Ile Ile Arg
355 360 365
Cys Tyr Ser Glu Glu Asp Phe Ser His Phe Pro Arg Phe Val Thr Pro
370 375 380
Glu Ile Ser Ser Ala Asp Leu Thr Gln Ala Ala Leu Trp Leu Ala Gln
385 390 395 400
Trp Gly Thr Ser Pro Ala Asp Leu Pro Leu Leu Asp Gln Pro Pro His
405 410 415
Ala Ala Trp Thr Ala Ala Gln Gln Ile Leu Arg Leu Ile Gly Ala Leu
420 425 430
Glu Gly Asp Ala Ile Thr Ser Leu Gly His Arg Leu Ser Thr Leu Pro
435 440 445
Leu Cys Pro Gln Leu Ser Ala Ser Leu Leu Arg Phe Gly Glu Gln Ser
450 455 460
Ala Lys Ile Leu Ala Val Val Ser Glu Asn Pro Gln Gly Asp Val Glu
465 470 475 480
Lys Gln Gln Pro Asp Lys Arg Glu Val Glu Arg Leu Arg Arg Leu Ala
485 490 495
Pro Ala Ser Val Gly Lys Ala Ser Ala Gly Gln Ile Val Gly Ala Ala
500 505 510
Phe Pro Gln Leu Ile Gly Arg Lys Ile Asp Asn Gly Glu Tyr Leu Leu
515 520 525
Ala Ser Gly Thr Arg Ala Arg Leu Met Asp Ser Asp Leu Lys Asp Ala
530 535 540
Glu Trp Ile Ser Val Ala Ala Ile Asn Arg Ser Gln Asn Ser Ala Ile
545 550 555 560
Ile Arg Ala Ala Ala Arg Ile Ser Glu Asp Asp Ala Ile Asp Ile Ile
565 570 575
Gly Val Val Glu Glu Thr Arg Ala Ile Phe Val Asn Gly Lys Val Gln
580 585 590
Ala Arg Lys Val Lys Ala Ala Gly Ala Ile Glu Leu Ser Ser Thr Pro
595 600 605
Thr Lys Pro Thr Pro Ala Glu Ala Ser Glu Thr Ile Ala Thr Ala Leu
610 615 620
Ala Lys Gly Gly Ile Asp Leu Phe His Phe Ser Asp Lys Ala Ala Ser
625 630 635 640
Leu Arg Asp Arg Leu Lys Phe Ile His Glu His Arg Gly Glu Pro Trp
645 650 655
Pro Asp Ile Glu Thr Ala Asp Pro His Leu Trp Leu Ser Pro Glu Ile
660 665 670
Glu Ala Leu Ser His Gly Thr Arg Leu Asn Asn Ile Asp Met Tyr Pro
675 680 685
Ala Leu Gln Arg Leu Leu Pro Trp Pro Glu Ala Thr Asn Phe Glu Glu
690 695 700
Phe Ala Pro Ser His Leu Ser Val Pro Ser Gly Asn Gln His Arg Leu
705 710 715 720
Asp Tyr Ser Ser Gly Arg Pro Val Ile Arg Val Lys Leu Gln Glu Cys
725 730 735
Phe Gly Leu Glu Glu Ser Pro Gln Leu Cys Gly Ile Pro Val Gln Phe
740 745 750
His Leu Leu Ser Pro Ala Gly Arg Pro Leu Ala Val Thr Asp Asp Leu
755 760 765
Arg Ser Phe Trp Ser Gly Pro Tyr Ser Gln Val Arg Ala Glu Met Arg
770 775 780
Gly Arg Tyr Pro Lys His Pro Trp Pro Glu Asp Pro Trp Thr Ala Pro
785 790 795 800
Ala Thr Ala Arg Thr Lys Asn Arg Met
805
<210>59
<211>6430
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(6400)
<223>RXA02357
<400>59
tatacctttg aatatccttt ccgggtgttg gatcgtgaag ctgatctgcg tgctgcatat 60
gcaaaatttg aaaaccagct aaaggagcca tagagcgctt atg tcc tca ctc att 115
Met Ser Ser Leu Ile
1 5
cct gtc cat gcg gct ggc agc att caa gaa ggc atc acc gaa tat ttg 163
Pro Val His Ala Ala Gly Ser Ile Gln Glu Gly Ile Thr Glu Tyr Leu
10 15 20
acc acc agt ttt tcc ctt gcg gat aag caa gtg gcc acc gag ctc aag 21l
Thr Thr Ser Phe Ser Leu Ala Asp Lys Gln Val Ala Thr Glu Leu Lys
25 30 35
cgg ttc ttg ggc cac ggt gat tcc ggc atg ttc cac ggg cct tat gtg 259
Arg Phe Leu Gly His Gly Asp Ser Gly Met Phe His Gly Pro Tyr Val
40 45 50
cgt gca cgt ttg ccc tat gct cag gca cag gaa tgg gaa aat gtg ctg 307
Arg Ala Arg Leu Pro Tyr Ala Gln Ala Gln Glu Trp Glu Asn Val Leu
55 60 65
agt tgg tta cct gag aac ttt gtg ccc tat cac cat caa aaa gcg gcg 355
Ser Trp Leu Pro Glu Asn Phe Val Pro Tyr His His Gln Lys Ala Ala
70 75 80 85
ttt cag cgg ctg agt tcc ctc gat aac cga ggt aaa gat cgc cgc ccc 403
Phe Gln Arg Leu Ser Ser Leu Asp Asn Arg Gly Lys Asp Arg Arg Pro
90 95 100
gat ccc acc ttg gtg gta act gga acg ggt tcc ggt aag acg gaa tct 451
Asp Pro Thr Leu Val Val Thr Gly Thr Gly Ser Gly Lys Thr Glu Ser
105 110 115
ttc ctc tac ccg att ctg gat cat gcc ctg cgc ctg cgg aaa cgt ggc 499
Phe Leu Tyr Pro Ile Leu Asp His Ala Leu Arg Leu Arg Lys Arg Gly
120 125 130
caa caa ggc atc aag gcg ctg ttg ctt tat ccc atg aac gcc ttg gcc 547
Gln Gln Gly Ile Lys Ala Leu Leu Leu Tyr Pro Met Asn Ala Leu Ala
135 140 145
aat gac cag gca gat cgt ttg gcg cgg ctc att cac aat aac cca gca 595
Asn Asp Gln Ala Asp Arg Leu Ala Arg Leu Ile His Asn Asn Pro Ala
150 155 160 165
ctt aaa ggt gtt acc gcc ggt att tat acc ggt gaa gcc aag ggc aat 543
Leu Lys Gly Val Thr Ala Gly Ile Tyr Thr Gly Glu Ala Lys Gly Asn
170 175 180
cgc acg cag atg ggc gaa agg gag ctt att aat gat ccc caa gcc atg 691
Arg Thr Gln Met Gly Glu Arg Glu Leu Ile Asn Asp Pro Gln Ala Met
185 190 195
cgg gtt agt ccc cca gat att ttg ctc acc aac tac aaa atg ttg gat 739
Arg Val Ser Pro Pro Asp Ile Leu Leu Thr Asn Tyr Lys Met Leu Asp
200 205 210
cag ctc ttg ctg cgc agc gtc gac cgc gaa atg tgg caa aaa tcc gcc 787
Gln Leu Leu Leu Arg Ser Val Asp Arg Glu Met Trp Gln Lys Ser Ala
215 220 225
acc tct ttg cag tat tta gta ctt gat gag ttc cac act tat gac ggt 835
Thr Ser Leu Gln Tyr Leu Val Leu Asp Glu phe His Thr Tyr Asp Gly
230 235 240 245
gct caa ggt aca gat gtg gcg ttg ttg ttg cgg cgt ttg ggg ctc atg 883
Ala Gln Gly Thr Asp Val Ala Leu Leu Leu Arg Arg Leu Gly Leu Met
250 255 260
ctg aaa tcc cag cag cct gct aat ttt ttg gat gat agt gcc atg cac 931
Leu Lys Ser Gln Gln Pro Ala Asn Phe Leu Asp Asp Ser Ala Met His
265 270 275
cgg cca ttg ggc att att acg cca gtg gct acc tca gca acc ttg ggt 979
Arg Pro Leu Gly Ile Ile Thr Pro Val Ala Thr Ser Ala Thr Leu Gly
280 285 290
tct ggt gat tcc ggc tcc ccc atg ttg gat ttt gct tac acc att ttt 1027
Ser Gly Asp Ser Gly Ser Pro Met Leu Asp Phe Ala Tyr Thr Ile Phe
295 300 305
ggt gag cgt ttt cca gct gat gcc atc gtc ggg gaa act cgc ctg gag 1075
Gly Glu Arg Phe Pro Ala Asp Ala Ile Val Gly Glu Thr Arg Leu Glu
310 315 320 325
ctg gat caa tgg cgc gca gaa att gcc cag aat ttt ggt gcc cca gca 1123
Leu Asp Gln Trp Arg Ala Glu Ile Ala Gln Asn Phe Gly Ala Pro Ala
330 335 340
gtt agc gag ccc cgt gag cta ccc act gtg gaa gat att gag gtg gtt 1171
Val Ser Glu Pro Arg Glu Leu Pro Thr Val Glu Asp Ile Glu Val Val
345 350 355
tta gat acc atc gcc aca gct caa cat gag gac gat tat gca cag ctc 1219
Leu Asp Thr Ile Ala Thr Ala Gln His Glu Asp Asp Tyr Ala Gln Leu
360 365 370
tgc ttc cgc gtt ttt tgt gag aaa gtg tgg ctg tgt gag gct gat ctg 1267
Cys Phe Arg Val Phe Cys Glu Lys Val Trp Leu Cys Glu Ala Asp Leu
375 380 385
cac gca gcg atc agt gct tat gcg gct cat gat tta act gct gcg att 1315
His Ala Ala Ile Ser Ala Tyr Ala Ala His Asp Leu Thr Ala Ala Ile
390 395 400 405
ttg cag cat gct gct gat tcc acc ccg ctt agc agg cgt gat caa gat 1363
Leu Gln His Ala Ala Asp Ser Thr Pro Leu Ser Arg Arg Asp Gln Asp
410 415 420
gag gtc act gcc ctt ccg gag ctg gta ttg ggg gct acc gcc cgc atc 1411
Glu Val Thr Ala Leu Pro Glu Leu Val Leu Gly Ala Thr Ala Arg Ile
425 430 435
tta ggt gag gtt aaa gct gcg gaa ttt atc agc cat gca ctg gct gcc 1459
Leu Gly Glu Val Lys Ala Ala Glu Phe Ile Ser His Ala Leu Ala Ala
440 445 450
atg gca ttt gtg cgc gcg gaa tac ggc aaa gtt gcc gcc tgg ggt gcc 1507
Met Ala Phe Val Arg Ala Glu Tyr Gly Lys Val Ala Ala Trp Gly Ala
455 460 465
aag cgc cta cct ggc gtc gaa acg cat ctt tgg gtg cgc gag gtc tcc 1555
Lys Arg Leu Pro Gly Val Glu Thr His Leu Trp Val Arg Glu Val Ser
470 475 480 485
cgc att gac cgc gcc ctg ggg gtt ggg gat gaa caa tcc atg ttt cgc 1603
Arg Ile Asp Arg Ala Leu Gly Val Gly Asp Glu Gln Ser Met Phe Arg
490 495 500
tgg tcc gat gat ggc ccg gca gaa gat gcc aat acc caa caa tgg ttg 1651
Trp Ser Asp Asp Gly Pro Ala Glu Asp Ala Asn Thr Gln Gln Trp Leu
505 510 515
ccg gcg tgt tat tgc cgc agt tgt ggc cgt tcc ggc tgg atg gtc agc 1699
Pro Ala Cys Tyr Cys Arg Ser Cys Gly Arg Ser Gly Trp Met Val Ser
520 525 530
ctg gaa caa ggc acc aat atc ccg gtg ttg gaa gaa caa aaa att cgc 1747
Leu Glu Gln Gly Thr Asn Ile Pro Val Leu Glu Glu Gln Lys Ile Arg
535 540 545
ctc aac tcc ttt gaa caa ccc cat aaa caa aga gcg ctt ctc gac gcc 1795
Leu Asn Ser Phe Glu Gln Pro His Lys Gln Arg Ala Leu Leu Asp Ala
550 555 560 565
acc tct gag cag cgc gca gcc att gag cag ggg cgc agc gtt gct ggg 1843
Thr Ser Glu Gln Arg Ala Ala Ile Glu Gln Gly Arg Ser Val Ala Gly
570 575 580
ccg cga gga gtt gat ggc acc tca gca gtg ctg tgg ttc cat agc gcc 1891
Pro Arg Gly Val Asp Gly Thr Ser Ala Val Leu Trp Phe His Ser Ala
585 590 595
agc aat gaa ctc agc acg cgc caa ccc agc cca gaa gaa gaa caa tca 1939
Ser Asn Glu Leu Ser Thr Arg Gln Pro Ser Pro Glu Glu Glu Gln Ser
600 605 610
ggt tct tct atc gca gtg ctc acc cac ttt ggc cct gag gca gac gat 1987
Gly Ser Ser Ile Ala Val Leu Thr His Phe Gly Pro Glu Ala Asp Asp
615 620 625
ctc tcc gcc aaa cag acc tgc cct tcc tgt ggt gat gtt gat tcc atc 2035
Leu Ser Ala Lys Gln Thr Cys Pro Ser Cys Gly Asp Val Asp Ser Ile
630 635 640 645
cgt tat atc gga tcg gga atc tct acc ctg ctc tct gtc tca ctc tcc 2083
Arg Tyr Ile Gly Ser Gly Ile Ser Thr Leu Leu Ser Val Ser Leu Ser
650 655 660
aat ctt ttt ggt atg gcc gat ttg gat agc gct gag aaa aag acg ctg 2131
Asn Leu Phe Gly Met Ala Asp Leu Asp Ser Ala Glu Lys Lys Thr Leu
665 670 675
gtc ttt gcc gat tcc gtg caa gat gcc gcg cac cgc gcc ggg tac gtc 2179
Val Phe Ala Asp Ser Val Gln Asp Ala Ala His Arg Ala Gly Tyr Val
680 685 690
caa gca cgt tcc cgc gct ttt gcc ctt cgt acc tat acc cgg cgc gca 2227
Gln Ala Arg Ser Arg Ala Phe Ala Leu Arg Thr Tyr Thr Arg Arg Ala
695 700 705
gtg ggt gat aat gaa gtc acc ttg cca tca atc tcc cgg gcg ctg atg 2275
Val Gly Asp Asn Glu Val Thr Leu Pro Ser Ile Ser Arg Ala Leu Met
710 715 720 725
gat aac gcc acc tct ggg cgc acc cgt tat gag ctc cta ccc cct gac 2323
Asp Asn Ala Thr Ser Gly Arg Thr Arg Tyr Glu Leu Leu Pro Pro Asp
730 735 740
ctg acc gat ctt gat att tac aaa cct tat tgg cac ccc gat gcc agc 2371
Leu Thr Asp Leu Asp Ile Tyr Lys Pro Tyr Trp His Pro Asp Ala Ser
745 750 755
aag gct gag cgc cgt gag gcc tcc cgc aat gtg cat aaa cgt tta agt 2419
Lys Ala Glu Arg Arg Glu Ala Ser Arg Asn Val His Lys Arg Leu Ser
760 765 770
ttt gat ctc gcc ctc gag ttt gga caa cgc gct gat cta ccc cgt tcc 2467
Phe Asp Leu Ala Leu Glu Phe Gly Gln Arg Ala Asp Leu Pro Arg Ser
775 780 785
ctt gcg ctg acc ggc gct ttg agt gct ttt gtg gat ttg ccc aaa ggt 2515
Leu Ala Leu Thr Gly Ala Leu Ser Ala Phe Val Asp Leu Pro Lys Gly
790 795 800 805
gtt gca ttg tcc gcg gct gcc gaa gca ctt tat gcc atc gaa gtt ccc 2563
Val Ala Leu Ser Ala Ala Ala Glu Ala Leu Tyr Ala Ile Glu Val Pro
810 815 820
acc tta gat att gag gat gag aac ctg cga ctg cgc tgg gtg caa ggt 2611
Thr Leu Asp Ile Glu Asp Glu Asn Leu Arg Leu Arg Trp Val Gln Gly
825 830 835
gcc ctg gaa ctt ttg cgc gcc cgc ggg ggc atc aac cat gag tgg ttt 2659
Ala Leu Glu Leu Leu Arg Ala Arg Gly Gly Ile Asn His Glu Trp Phe
840 845 850
ggc gcc tac ctg cgc acc gat ggc aac ccc tat atg ctt aac cgc cgc 2707
Gly Ala Tyr Leu Arg Thr Asp Gly Asn Pro Tyr Met Leu Asn Arg Arg
855 860 865
cas gcc cgc gct gag ggc att ccc ggt ttt gtc cgc ggt ggt gca cct 2755
Gln Ala Arg Ala Glu Gly Ile Pro Gly Phe Val Arg Gly Gly Ala Pro
870 875 880 885
gaa ttc cca cgt gta ggt tcc gca ctt tcc gga tcc ctg cgt tcc agc 2803
Glu Phe Pro Arg Val Gly Ser Ala Leu Ser Gly Ser Leu Arg Ser Ser
890 895 900
acc ggc acc acc ccg ctt ggt agc ccg cgc gga cgt tat gcg tcc tgg 2851
Thr Gly Thr Thr Pro Leu Gly Ser Pro Arg Gly Arg Tyr Ala Ser Trp
905 910 915
acc tcg cag gtg ctt ggc atc agc acc cac gat gcc gcc acc gcc atc 2899
Thr Ser Gln Val Leu Gly Ile Ser Thr His Asp Ala Ala Thr Ala Ile
920 925 930
aca aag ctt ttc gac gct tta agc aac cgc agc att ctc tcc tca ata 2947
Thr Lys Leu Phe Asp Ala Leu Ser Asn Arg Ser Ile Leu Ser Ser Ile
935 940 945
tct acc gac agc ggc gga aaa atc tac tgc ctc gag gcc gaa cgc atc 2995
Ser Thr Asp Ser Gly Gly Lys Ile Tyr Cys Leu Glu Ala Glu Arg Ile
950 955 960 965
cgt att ttt agc gaa gac cat ccc gaa gtt ctg gaa tgc agc gtg tgc 3043
Arg Ile Phe Ser Glu Asp His Pro Glu Val Leu Glu Cys Ser Val Cys
970 975 980
cat gcc caa act ggt gta act gat cat gtg cgt gaa ctt ctt gat ggc 3091
His Ala Gln Thr Gly Val Thr Asp His Val Arg Glu Leu Leu Asp Gly
985 990 995
gct ccg tgt ttt agc cct agt tgt ggg ggc gtt ctc cat acc gag gaa 3139
Ala Pro Cys Phe Ser Pro Ser Cys Gly Gly Val Leu His Thr Glu Glu
1000 1005 1010
gta gaa gac aac tac tac cgc agg ctt tat tcc gca att gaa ccg cgc 3187
Val Glu Asp Asn Tyr Tyr Arg Arg Leu Tyr Ser Ala Ile Glu Pro Arg
1015 1020 1025
act gtc att gcc cgc gag cac acc agc atg ctc aag aea aaa gac cgc 3235
Thr Val Ile Ala Arg Glu His Thr Ser Met Leu Lys Lys Lys Asp Arg
1030 1035 1040 1045
ctc gca ctc gaa caa tcc ttt aga ggt gga gaa ggc tcg gcc aaa caa 3283
Leu Ala Leu Glu Gln Ser Phe Arg Gly Gly Glu Gly Ser Ala Lys Gln
1050 1055 1060
tcc ccc gat gcg ccc aat gtg ttg gta gct acg cca acg ctg gaa atg 3331
Ser Pro Asp Ala Pro Asn Val Leu Val Ala Thr Pro Thr Leu Glu Met
1065 1070 1075
ggt att gac atc ggc gat ctc tcc acc gtg atg ctt gcg tct ttg cca 3379
Gly Ile Asp Ile Gly Asp Leu Ser Thr Val Met Leu Ala Ser Leu Pro
1080 1085 1090
aca tca gtg gcc agt tat gta cag cgt gtt ggt cgt gcc ggg cgc ctc 3427
Thr Ser Val Ala Ser Tyr Val Gln Arg Val Gly Arg Ala Gly Arg Leu
1095 1100 1105
agc gga aac tcg ttg gta ctc gcc gtg gtg cgt ggc cgc ggt gtc aca 3475
Ser Gly Asn Ser Leu Val Leu Ala Val Val Arg Gly Arg Gly Val Thr
1110 1115 1120 1125
ttg cct cgt ctt aac caa ccg ctc tcc atg atc aag gga gct att acc 3523
Leu Pro Arg Leu Asn Gln Pro Leu Ser Met Ile Lys Gly Ala Ile Thr
1130 1135 1140
cca cca gtt gcg tat ctt tcc gcc agt gaa atc ttg cac cgc caa ttc 3571
Pro Pro Val Ala Tyr Leu Ser Ala Ser Glu Ile Leu His Arg Gln Phe
1145 1150 1155
ttg gca tat gtg atc gac tgc tta gat act cgc gcc gaa ctg cca aaa 3619
Leu Ala Tyr Val Ile Asp Cys Leu Asp Thr Arg Ala Glu Leu Pro Lys
1160 1165 1170
ctc gaa aca gca att gat gtt ttt gat aat gcc gcc gga aaa aca cct 3667
Leu Glu Thr Ala Ile Asp Val Phe Asp Asn Ala Ala Gly Lys Thr Pro
1175 1180 1185
ttg gtt gca tta ctc aaa gcg cag att cac gca ggc cta gat cct ttg 3715
Leu Val Ala Leu Leu Lys Ala Gln Ile His Ala Gly Leu Asp Pro Leu
1190 1195 1200 1205
ctg gag gaa ttt gta cgc acc ctc aac atg caa atc agc att gat aat 3763
Leu Glu Glu Phe Val Arg Thr Leu Asn Met Gln Ile Ser Ile Asp Asn
1210 1215 1220
att ttc gaa ctg cgc act tgg gca tct gga aat agc aca gac tcc ctc 3811
Ile Phe Glu Leu Arg Thr Trp Ala Ser Gly Asn Ser Thr Asp Ser Leu
1225 1230 1235
ctc gcg ctg ctg gaa acc tcc caa aaa gag tgg atg gaa gaa cgc cgc 3859
Leu Ala Leu Leu Glu Thr Ser Gln Lys Glu Trp Met Glu Glu Arg Arg
1240 1245 1250
agc ctt acc gcg cgg cgg gga gaa ctc gaa aaa atc ttt gac aaa cta 3907
Ser Leu Thr Ala Arg Arg Gly Glu Leu Glu Lys Ile Phe Asp Lys Leu
1255 1260 1265
gat gcg cgc aat gat gca cat gat gaa gag cta aag gaa gaa aaa cgt 3955
Asp Ala Arg Asn Asp Ala His Asp Glu Glu Leu Lys Glu Glu Lys Arg
1270 1275 1280 1285
aaa acc gca gct tcc ctt aaa gcc gtc aaa ctt caa atc cgt gat cta 4003
Lys Thr Ala Ala Ser Leu Lys Ala Val Lys Leu Gln Ile Arg Asp Leu
1290 1295 1300
ctt ggt gaa ttc tgg atc gct gct ttg gag cgt tat ggt ctc ctg ccc 4051
Leu Gly Glu Phe Trp Ile Ala Ala Leu Glu Arg Tyr Gly Leu Leu Pro
1305 1310 1315
aac ttc acc ctg gtt gat gat tcc gta gaa ctt aat gtg gcg gtt act 4099
Asn Phe Thr Leu Val Asp Asp Ser Val Glu Leu Asn Val Ala Val Thr
1320 1325 1330
tcg ttc aac ccc cag gaa gtg gaa ttt gat acc aag aat cat gcc tac 4147
Ser Phe Asn Pro Gln Glu Val Glu Phe Asp Thr Lys Asn His Ala Tyr
1335 1340 1345
tcg cgt ggg atc tct gca gcg ctt ttt gag ctc gct ccc ggt gcg acc 4195
Ser Arg Gly Ile Ser Ala Ala Leu Phe Glu Leu Ala Pro Gly Ala Thr
1350 1355 1360 1365
ttt tat gcc caa gga att gct gcc aaa gtt gat tcc att gag att ggt 4243
Phe Tyr Ala Gln Gly Ile Ala Ala Lys Val Asp Ser Ile Glu Ile Gly
1370 1375 1380
gaa cat ggc tct gcc att gag caa tgg cgg ttg tgc ccc gtg tgc tcg 4291
Glu His Gly Ser Ala Ile Glu Gln Trp Arg Leu Cys Pro Val Cys Ser
1385 1390 1395
cac tcc gaa atc ctc cag ccg ggt gtc tct aca ccg gga tcg tgt cct 4339
His Ser Glu Ile Leu Gln Pro Gly Val Ser Thr Pro Gly Ser Cys Pro
1400 1405 1410
acc tgt ggc tcc ccc gcc ttt gcc gat aag gga caa atc cta gag gta 4387
Thr Cys Gly Ser Pro Ala Phe Ala Asp Lys Gly Gln Ile Leu Glu Val
1415 1420 1425
gtg caa atg cgt aag gtt tcc tcc gca gtg gag aaa act cgc gct gcc 4435
Val Gln Met Arg Lys Val Ser Ser Ala Val Glu Lys Thr Arg Ala Ala
1430 1435 1440 1445
att agc gat gac cgt gaa gat cgc ttt agc acc cgc ttt aac cag cac 4483
Ile Ser Asp Asp Arg Glu Asp Arg Phe Ser Thr Arg Phe Asn Gln His
1450 1455 1460
gtc agc ttt gtc gtt cca cca gat ggc cat gga aaa tcc tgg tat ttg 4531
Val Ser Phe Val Val Pro Pro Asp Gly His Gly Lys Ser Trp Tyr Leu
1465 1470 1475
aat gat ggt ttt ggc att gag cat ctg ccc aag gtg gaa tta cgc tgg 4579
Asn Asp Gly Phe Gly Ile Glu His Leu Pro Lys Val Glu Leu Arg Trp
1480 1485 1490
ctg aac tta gga att ggc aat ggc caa aag cgt cgg ctc ggt ggc ttt 4627
Leu Asn Leu Gly Ile Gly Asn Gly Gln Lys Arg Arg Leu Gly Gly Phe
1495 1500 1505
gaa gtc acc agc cca cta ttt aat gtg tgc cgc cac tgt gga cat tta 4675
Glu Val Thr Ser Pro Leu Phe Asn Val Cys Arg His Cys Gly His Leu
1510 1515 1520 1525
gat tcc gag gca gga gct aac tca cgc tgg gat cac cga ccc tgg tgc 4723
Asp Ser Glu Ala Gly Ala Asn Ser Arg Trp Asp His Arg Pro Trp Cys
1530 1535 1540
cca cac cgt tat gag caa aaa gaa gat acc gta tct ttc gct ttg ggt 4771
Pro His Arg Tyr Glu Gln Lys Glu Asp Thr Val Ser Phe Ala Leu Gly
1545 1550 1555
cgt act ctt aaa acc caa ggc gtg ctc atg ctg ctg ccc gaa tat ttt 4819
Arg Thr Leu Lys Thr Gln Gly Val Leu Met Leu Leu Pro Glu Tyr Phe
1560 1565 1570
ggc agt gaa gcc gat tct atg gtg gta acc agc ctc att gct gct atc 4867
Gly Ser Glu Ala Asp Ser Met Val Val Thr Ser Leu Ile Ala Ala Ile
1575 1580 1585
aaa tta gga ttt cgt gag gtg ttg ggt ggc gat cct gac cac ctg gat 4915
Lys Leu Gly Phe Arg Glu Val Leu Gly Gly Asp Pro Asp His Leu Asp
1590 1595 1600 1605
gtc acc agt gtg cag gtc ccc cgt act tct ggc gat ggt gca ctt gat 4963
Val Thr Ser Val Gln Val Pro Arg Thr Ser Gly Asp Gly Ala Leu Asp
1610 1615 1620
gcc ctt ttg ctg cac gat cag gtt cca gga ggc acg ggt tat ctt aac 5011
Ala Leu Leu Leu His Asp Gln Val Pro Gly Gly Thr Gly Tyr Leu Asn
1625 1630 1635
caa ttt gcc gat cct aca aag gtt cgt gaa ctt att tcc cga gct tgg 5059
Gln Phe Ala Asp Pro Thr Lys Val Arg Glu Leu Ile Ser Arg Ala Trp
1640 1645 1650
gag cgg gtg tct agg tgc caa tgc cag tat gat gaa agg ctg gcc tgc 5107
Glu Arg Val Ser Arg Cys Gln Cys Gln Tyr Asp Glu Arg Leu Ala Cys
1655 1660 1665
cca gaa tgt ttg ttg cct tat acc cgc act gcc acg ctc ctc cag act 5155
Pro Glu Cys Leu Leu Pro Tyr Thr Arg Thr Ala Thr Leu Leu Gln Thr
1670 1675 1680 1685
tcc cgc gca gct gca gaa aaa gcc ttg cgc gca att ttg ctc aat agc 5203
Ser Arg Ala Ala Ala Glu Lys Ala Leu Arg Ala Ile Leu Leu Asn Ser
1690 1695 1700
tcg cgc ccc gaa gaa atc act gac ctt tct gca gta ccg gac tgg act 5251
Ser Arg Pro Glu Glu Ile Thr Asp Leu Ser Ala Val Pro Asp Trp Thr
1705 1710 1715
ttc ttg gaa aag cgc cca gag aac act ctt ggc tct cag ttg gaa ctg 5299
Phe Leu Glu Lys Arg Pro Glu Asn Thr Leu Gly Ser Gln Leu Glu Leu
1720 1725 1730
cgt ttc cgc gtg atg ctg cga cgc gcc tta aaa aat cgc cat gcc aaa 5347
Arg Phe Arg Val Met Leu Arg Arg Ala Leu Lys Asn Arg His Ala Lys
1735 1740 1745
ctt gtg gac cgc gtc aac ggc tca aac tct tat gtg gat att gag atg 5395
Leu Val Asp Arg Val Asn Gly Ser Asn Ser Tyr Val Asp Ile Glu Met
1750 1755 1760 1765
agc tcc ggt gtg cgc tgg cgg atg agc gaa caa gtt gat cgt gga tat 5443
Ser Ser Gly Val Arg Trp Arg Met Ser Glu Gln Val Asp Arg Gly Tyr
1770 1775 1780
aca cgc cct gat ttc tgg ttt gaa ccg ctc aac ggc aac tat ccc acc 5491
Thr Arg Pro Asp Phe Trp Phe Glu Pro Leu Asn Gly Asn Tyr Pro Thr
1785 1790 1795
gtg gct gtt ttt acc gat ggc gct gcg ttc cat atc tct tca gct aac 5539
Val Ala Val Phe Thr Asp Gly Ala Ala Phe His Ile Ser Ser Ala Asn
1800 1805 1810
tac cgt ctt gat ggc gat att cag aaa cgg atg aaa cta gcg ctc gat 5587
Tyr Arg Leu Asp Gly Asp Ile Gln Lys Arg Met Lys Leu Ala Leu Asp
1815 1820 1825
cca gac aat att ttg ccg tgg aat atc act agc tta gac ctc gac cgc 5635
Pro Asp Asn Ile Leu Pro Trp Asn Ile Thr Ser Leu Asp Leu Asp Arg
1830 1835 1840 1845
ttt agt aat ccc gct gca caa ggt gag gaa cca gca tgg ttt agc ccc 5633
Phe Ser Asn Pro Ala Ala Gln Gly Glu Glu Pro Ala Trp Phe Ser Pro
1850 1855 1860
atc ggc agg cag ctc agc aaa gca aat ttg att ctt gat cca caa tca 5731
Ile Gly Arg Gln Leu Ser Lys Ala Asn Leu Ile Leu Asp Pro Gln Ser
l865 1870 1875
act gca ctc ctg gca gca acg cct atg gat caa ctc ttg gca ttt tta 5779
Thr Ala Leu Leu Ala Ala Thr Pro Met Asp Gln Leu Leu Ala Phe Leu
1880 1885 1890
gat aat ccc gcg gca tcc tcg tgg aag gag ttt gct cat atc gct gct 5827
Asp Asn Pro Ala Ala Ser Ser Trp Lys Glu Phe Ala His Ile Ala Ala
1895 1900 1905
gct cac atg ctt ggg cat aat cca caa aaa aat ggc gac gga att gtt 5875
Ala His Met Leu Gly His Asn Pro Gln Lys Asn Gly Asp Gly Ile Val
1910 1915 1920 1925
ggt acc ttc cgc aat aag att tcc ttg cgg gcc acc atg gtt aat cgg 5923
Gly Thr Phe Arg Asn Lys Ile Ser Leu Arg Ala Thr Met Val Asn Arg
1930 1935 1940
gaa ctg cgc gcc cgc caa ttg tgg ctt gct ccc acc act cca gaa gag 5971
Glu Leu Arg Ala Arg Gln Leu Trp Leu Ala Pro Thr Thr Pro Glu Glu
1945 1950 1955
ctg gaa gtg gat acc tgg act gcc ttc ctc aat ttg gcc aac ctc atg 6019
Leu Glu Val Asp Thr Trp Thr Ala Phe Leu Asn Leu Ala Asn Leu Met
1960 1965 1970
tgg ctg gca ccg gaa tcc gta gac gta agc act aat ggt tca ccg cat 6067
Trp Leu Ala Pro Glu Ser Val Asp Val Ser Thr Asn Gly Ser Pro His
1975 1980 1985
aaa att gat att gtg cct gct cca gca gct ccg ctt gct gtt gaa gtt 6115
Lys Ile Asp Ile Val Pro Ala Pro Ala Ala Pro Leu Ala Val Glu Val
1990 1995 2000 2005
cct gaa ttg tgg gct ccc atc ttg gat ggc ttt acc gcc gat gaa gat 6163
Pro Glu Leu Trp Ala Pro Ile Leu Asp Gly Phe Thr Ala Asp Glu Asp
2010 2015 2020
gaa gaa gcc gaa ggc gct ttg cag atc ttg gct aag gaa cat gcc ctg 6211
Glu Glu Ala Glu Gly Ala Leu Gln Ile Leu Ala Lys Glu His Ala Leu
2025 2030 2035
gtt cca gag acc acc ggt gat gag ctc tcg agt att cct acg att gct 6259
Val Pro Glu Thr Thr Gly Asp Glu Leu Ser Ser Ile Pro Thr Ile Ala
2040 2045 2050
acg tgg ccg agt gtc aag atc gct ttg ctc tat gaa tct gat ccc gat 6307
Thr Trp Pro Ser Val Lys Ile Ala Leu Leu Tyr Glu Ser Asp Pro Asp
2055 2060 2065
gag cct ttg gag gat gat ctc aaa gct gaa ggt tgg acg ctg ctt ttt 6355
Glu Pro Leu Glu Asp Asp Leu Lys Ala Glu Gly Trp Thr Leu Leu Phe
2070 2075 2080 2085
gca aat gac ctc gag acc tcc gat att ccc gct gcc ctt cgt ccc 6400
Ala Asn Asp Leu Glu Thr Ser Asp Ile Pro Ala Ala Lau Arg Pro
2090 2095 2100
tagtacaccc aaagtttatg aaagataaaa 6430
<210>60
<211>2100
<212>PRT
<213>谷氨酸棒状杆菌
<400>60
Met Ser Ser Leu Ile Pro Val His Ala Ala Gly Ser Ile Gln Glu Gly
1 5 10 15
Ile Thr Glu Tyr Leu Thr Thr Ser Phe Ser Leu Ala Asp Lys Gln Val
20 25 30
Ala Thr Glu Leu Lys Arg Phe Leu Gly His Gly Asp Ser Gly Met Phe
35 40 45
His Gly Pro Tyr Val Arg Ala Arg Leu Pro Tyr Ala Gln Ala Gln Glu
50 55 60
Trp Glu Asn Val Leu Ser Trp Leu Pro Glu Asn Phe Val Pro Tyr His
65 70 75 80
His Gln Lys Ala Ala Phe Gln Arg Leu Ser Ser Leu Asp Asn Arg Gly
85 90 95
Lys Asp Arg Arg Pro Asp Pro Thr Leu Val Val Thr Gly Thr Gly Ser
100 105 110
Gly Lys Thr Glu Ser Phe Leu Tyr Pro Ile Leu Asp His Ala Leu Arg
115 120 125
Leu Arg Lys Arg Gly Gln Gln Gly Ile Lys Ala Leu Leu Leu Tyr Pro
130 135 140
Met Asn Ala Leu Ala Asn Asp Gln Ala Asp Arg Leu Ala Arg Leu Ile
145 150 155 160
His Asn Asn Pro Ala Leu Lys Gly Val Thr Ala Gly Ile Tyr Thr Gly
165 170 175
Glu Ala Lys Gly Asn Arg Thr Gln Met Gly Glu Arg Glu Leu Ile Asn
180 185 190
Asp Pro Gln Ala Met Arg Val Ser Pro Pro Asp Ile Leu Leu Thr Asn
195 200 205
Tyr Lys Met Leu Asp Gln Leu Leu Leu Arg Ser Val Asp Arg Glu Met
210 215 220
Trp Gln Lys Ser Ala Thr Ser Leu Gln Tyr Leu Val Leu Asp Glu Phe
225 230 235 240
His Thr Tyr Asp Gly Ala Gln Gly Thr Asp Val Ala Leu Leu Leu Arg
245 250 255
Arg Leu Gly Leu Met Leu Lys Ser Gln Gln Pro Ala Asn Phe Leu Asp
260 265 270
Asp Ser Ala Met His Arg Pro Leu Gly Ile Ile Thr Pro Val Ala Thr
275 280 285
Ser Ala Thr Leu Gly Ser Gly Asp Ser Gly Ser Pro Met Leu Asp Phe
290 295 300
Ala Tyr Thr Ile Phe Gly Glu Arg Phe Pro Ala Asp Ala Ile Val Gly
305 310 315 320
Glu Thr Arg Leu Glu Leu Asp Gln Trp Arg Ala Glu Ile Ala Gln Asn
325 330 335
Phe Gly Ala Pro Ala Val Ser Glu Pro Arg Glu Leu Pro Thr Val Glu
340 345 350
Asp Ile Glu Val Val Leu Asp Thr Ile Ala Thr Ala Gln His Glu Asp
355 360 365
Asp Tyr Ala Gln Leu Cys Phe Arg Val Phe Cys Glu Lys Val Trp Leu
370 375 380
Cys Glu Ala Asp Leu His Ala Ala Ile Ser Ala Tyr Ala Ala His Asp
385 390 395 400
Leu Thr Ala Ala Ile Leu Gln His Ala Ala Asp Ser Thr Pro Leu Ser
405 410 415
Arg Arg Asp Gln Asp Glu Val Thr Ala Leu Pro Glu Leu Val Leu Gly
420 425 430
Ala Thr Ala Arg Ile Leu Gly Glu Val Lys Ala Ala Glu Phe Ile Ser
435 440 445
His Ala Leu Ala Ala Met Ala Phe Val Arg Ala Glu Tyr Gly Lys Val
450 455 460
Ala Ala Trp Gly Ala Lys Arg Leu Pro Gly Val Glu Thr His Leu Trp
465 470 475 480
Val Arg Glu Val Ser Arg Ile Asp Arg Ala Leu Gly Val Gly Asp Glu
485 490 495
Gln Ser Met Phe Arg Trp Ser Asp Asp Gly Pro Ala Glu Asp Ala Asn
500 505 510
Thr Gln Gln Trp Leu Pro Ala Cys Tyr Cys Arg Ser Cys Gly Arg Ser
515 520 525
Gly Trp Met Val Ser Leu Glu Gln Gly Thr Asn Ile Pro Val Leu Glu
530 535 540
Glu Gln Lys Ile Arg Leu Asn Ser Phe Glu Gln Pro His Lys Gln Arg
545 550 555 560
Ala Leu Leu Asp Ala Thr Ser Glu Gln Arg Ala Ala Ile Glu Gln Gly
565 570 575
Arg Ser Val Ala Gly Pro Arg Gly Val Asp Gly Thr Ser Ala Val Leu
580 585 590
Trp Phe His Ser Ala Ser Asn Glu Leu Ser Thr Arg Gln Pro Ser Pro
595 600 605
Glu Glu Glu Gln Ser Gly Ser Ser Ile Ala Val Leu Thr His Phe Gly
610 615 620
Pro Glu Ala Asp Asp Leu Ser Ala Lys Gln Thr Cys Pro Ser Cys Gly
625 630 635 640
Asp Val Asp Ser Ile Arg Tyr Ile Gly Ser Gly Ile Ser Thr Leu Leu
645 650 655
Ser Val Ser Leu Ser Asn Leu Phe Gly Met Ala Asp Leu Asp Ser Ala
660 665 670
Glu Lys Lys Thr Leu Val Phe Ala Asp Ser Val Gln Asp Ala Ala His
675 680 685
Arg Ala Gly Tyr Val Gln Ala Arg Ser Arg Ala Phe Ala Leu Arg Thr
690 695 700
Tyr Thr Arg Arg Ala Val Gly Asp Asn Glu Val Thr Leu Pro Ser Ile
705 710 715 720
Ser Arg Ala Leu Met Asp Asn Ala Thr Ser Gly Arg Thr Arg Tyr Glu
725 730 735
Leu Leu Pro Pro Asp Leu Thr Asp Leu Asp Ile Tyr Lys Pro Tyr Trp
740 745 750
His Pro Asp Ala Ser Lys Ala Glu Arg Arg Glu Ala Ser Arg Asn Val
755 760 765
His Lys Arg Leu Ser Phe Asp Leu Ala Leu Glu Phe Gly Gln Arg Ala
770 775 780
Asp Leu Pro Arg Ser Leu Ala Leu Thr Gly Ala Leu Ser Ala Phe Val
785 790 795 800
Asp Leu Pro Lys Gly Val Ala Leu Ser Ala Ala Ala Glu Ala Leu Tyr
805 810 815
Ala Ile Glu Val Pro Thr Leu Asp Ile Glu Asp Glu Asn Leu Arg Leu
820 825 830
Arg Trp Val Gln Gly Ala Leu Glu Leu Leu Arg Ala Arg Gly Gly Ile
835 840 845
Asn His Glu Trp Phe Gly Ala Tyr Leu Arg Thr Asp Gly Asn Pro Tyr
850 855 860
Met Leu Asn Arg Arg Gln Ala Arg Ala Glu Gly Ile Pro Gly Phe Val
865 870 875 880
Arg Gly Gly Ala Pro Glu Phe Pro Arg Val Gly Ser Ala Leu Ser Gly
885 890 895
Ser Leu Arg Ser Ser Thr Gly Thr Thr Pro Leu Gly Ser Pro Arg Gly
900 905 910
Arg Tyr Ala Ser Trp Thr Ser Gln Val Leu Gly Ile Ser Thr His Asp
915 920 925
Ala Ala Thr Ala Ile Thr Lys Leu Phe Asp Ala Leu Ser Asn Arg Ser
930 935 940
Ile Leu Ser Ser Ile Ser Thr Asp Ser Gly Gly Lys Ile Tyr Cys Leu
945 950 955 960
Glu Ala Glu Arg Ile Arg Ile Phe Ser Glu Asp His Pro Glu Val Leu
965 970 975
Glu Cys Ser Val Cys His Ala Gln Thr Gly Val Thr Asp His Val Arg
980 985 990
Glu Leu Leu Asp Gly Ala Pro Cys Phe Ser Pro Ser Cys Gly Gly Val
995 1000 1005
Leu His Thr Glu Glu Val Glu Asp Asn Tyr Tyr Arg Arg Leu Tyr Ser
1010 1015 1020
Ala Ile Glu Pro Arg Thr Val Ile Ala Arg Glu His Thr Ser Met Leu
1025 1030 1035 1040
Lys Lys Lys Asp Arg Leu Ala Leu Glu Gln Ser Phe Arg Gly Gly Glu
1045 1050 1055
Gly Ser Ala Lys Gln Ser Pro Asp Ala Pro Asn Val Leu Val Ala Thr
1060 1065 1070
Pro Thr Leu Glu Met Gly Ile Asp Ile Gly Asp Leu Ser Thr Val Met
1075 1080 1085
Leu Ala Ser Leu Pro Thr Ser Val Ala Ser Tyr Val Gln Arg Val Gly
1090 1095 1100
Arg Ala Gly Arg Leu Ser Gly Asn Ser Leu Val Leu Ala Val Val Arg
1105 1110 1115 1120
Gly Arg Gly Val Thr Leu Pro Arg Leu Asn Gln Pro Leu Ser Met Ile
1125 1130 1135
Lys Gly Ala Ile Thr Pro Pro Val Ala Tyr Leu Ser Ala Ser Glu Ile
1140 1145 1150
Leu His Arg Gln Phe Leu Ala Tyr Val Ile Asp Cys Leu Asp Thr Arg
1155 1160 1165
Ala Glu Leu Pro Lys Leu Glu Thr Ala Ile Asp Val Phe Asp Asn Ala
1170 1175 1180
Ala Gly Lys Thr Pro Leu Val Ala Leu Leu Lys Ala Gln Ile His Ala
1185 1190 1195 1200
Gly Leu Asp Pro Leu Leu Glu Glu Phe Val Arg Thr Leu Asn Met Gln
1205 1210 1215
Ile Ser Ile Asp Asn Ile Phe Glu Leu Arg Thr Trp Ala Ser Gly Asn
1220 1225 1230
Ser Thr Asp Ser Leu Leu Ala Leu Leu Glu Thr Ser Gln Lys Glu Trp
1235 1240 1245
Met Glu Glu Arg Arg Ser Leu Thr Ala Arg Arg Gly Glu Leu Glu Lys
1250 1255 1260
Ile Phe Asp Lys Leu Asp Ala Arg Asn Asp Ala His Asp Glu Glu Leu
1265 1270 1275 1280
Lys Glu Glu Lys Arg Lys Thr Ala Ala Ser Leu Lys Ala Val Lys Leu
1285 1290 1295
Gln Ile Arg Asp Leu Leu Gly Glu Phe Trp Ile Ala Ala Leu Glu Arg
1300 1305 1310
Tyr Gly Leu Leu Pro Asn Phe Thr Leu Val Asp Asp Ser Val Glu Leu
1315 1320 1325
Asn Val Ala Val Thr Ser Phe Asn Pro Gln Glu Val Glu Phe Asp Thr
1330 1335 1340
Lys Asn His Ala Tyr Ser Arg Gly Ile Ser Ala Ala Leu Phe Glu Leu
1345 1350 1355 1360
Ala Pro Gly Ala Thr Phe Tyr Ala Gln Gly Ile Ala Ala Lys Val Asp
1365 1370 1375
Ser Ile Glu Ile Gly Glu His Gly Ser Ala Ile Glu Gln Trp Arg Leu
1380 1385 1390
Cys Pro Val Cys Ser His Ser Glu Ile Leu Gln Pro Gly Val Ser Thr
1395 1400 1405
Pro Gly Ser Cys Pro Thr Cys Gly Ser Pro Ala Phe Ala Asp Lys Gly
1410 1415 1420
Gln Ile Leu Glu Val Val Gln Met Arg Lys Val Ser Ser Ala Val Glu
1425 1430 1435 1440
Lys Thr Arg Ala Ala Ile Ser Asp Asp Arg Glu Asp Arg Phe Ser Thr
1445 1450 1455
Arg Phe Asn Gln His Val Ser Phe Val Val Pro Pro Asp Gly His Gly
1460 1465 1470
Lys Ser Trp Tyr Leu Asn Asp Gly Phe Gly Ile Glu His Leu Pro Lys
1475 1480 1485
Val Glu Leu Arg Trp Leu Asn Leu Gly Ile Gly Asn Gly Gln Lys Arg
1490 1495 1500
Arg Leu Gly Gly Phe Glu Val Thr Ser Pro Leu Phe Asn Val Cys Arg
1505 1510 1515 1520
His Cys Gly His Leu Asp Ser Glu Ala Gly Ala Asn Ser Arg Trp Asp
1525 1530 1535
His Arg Pro Trp Cys Pro His Arg Tyr Glu Gln Lys Glu Asp Thr Val
1540 1545 1550
Ser Phe Ala Leu Gly Arg Thr Leu Lys Thr Gln Gly Val Leu Met Leu
1555 1560 1565
Leu Pro Glu Tyr Phe Gly Ser Glu Ala Asp Ser Met Val Val Thr Ser
1570 1575 1580
Leu Ile Ala Ala Ile Lys Leu Gly Phe Arg Glu Val Leu Gly Gly Asp
1585 1590 1595 1600
Pro Asp His Leu Asp Val Thr Ser Val Gln Val Pro Arg Thr Ser Gly
1605 1610 1615
Asp Gly Ala Leu Asp Ala Leu Leu Leu His Asp Gln Val Pro Gly Gly
1620 1625 1630
Thr Gly Tyr Leu Asn Gln Phe Ala Asp Pro Thr Lys Val Arg Glu Leu
1635 1640 1645
Ile Ser Arg Ala Trp Glu Arg Val Ser Arg Cys Gln Cys Gln Tyr Asp
1650 1655 1660
Glu Arg Leu Ala Cys Pro Glu Cys Leu Leu Pro Tyr Thr Arg Thr Ala
1665 1670 1675 1680
Thr Leu Leu Gln Thr Ser Arg Ala Ala Ala Glu Lys Ala Leu Arg Ala
1685 1690 1695
Ile Leu Leu Asn Ser Ser Arg Pro Glu Glu Ile Thr Asp Leu Ser Ala
1700 1705 1710
Val Pro Asp Trp Thr Phe Leu Glu Lys Arg Pro Glu Asn Thr Leu Gly
1715 1720 1725
Ser Gln Leu Glu Leu Arg Phe Arg Val Met Leu Arg Arg Ala Leu Lys
1730 1735 1740
Asn Arg His Ala Lys Leu Val Asp Arg Val Asn Gly Ser Asn Ser Tyr
1745 1750 1755 1760
Val Asp Ile Glu Met Ser Ser Gly Val Arg Trp Arg Met Ser Glu Gln
1765 1770 1775
Val Asp Arg Gly Tyr Thr Arg Pro Asp Phe Trp Phe Glu Pro Leu Asn
1780 1785 1790
Gly Asn Tyr Pro Thr Val Ala Val Phe Thr Asp Gly Ala Ala Phe His
1795 1800 1805
Ile Ser Ser Ala Asn Tyr Arg Leu Asp Gly Asp Ile Gln Lys Arg Met
1810 1815 1820
Lys Leu Ala Leu Asp Pro Asp Asn Ile Leu Pro Trp Asn Ile Thr Ser
1825 1830 1835 1840
Leu Asp Leu Asp Arg Phe Ser Asn Pro Ala Ala Gln Gly Glu Glu Pro
1845 1850 1855
Ala Trp Phe Ser Pro Ile Gly Arg Gln Leu Ser Lys Ala Asn Leu Ile
1860 1865 1870
Leu Asp Pro Gln Ser Thr Ala Leu Leu Ala Ala Thr Pro Met Asp Gln
1875 1880 1885
Leu Leu Ala Phe Leu Asp Asn Pro Ala Ala Ser Ser Trp Lys Glu Phe
1890 1895 1900
Ala His Ile Ala Ala Ala His Met Leu Gly His Asn Pro Gln Lys Asn
1905 1910 1915 1920
Gly Asp Gly Ile Val Gly Thr Phe Arg Asn Lys Ile Ser Leu Arg Ala
1925 1930 1935
Thr Met Val Asn Arg Glu Leu Arg Ala Arg Gln Leu Trp Leu Ala Pro
1940 1945 1950
Thr Thr Pro Glu Glu Leu Glu Val Asp Thr Trp Thr Ala Phe Leu Asn
1955 1960 1965
Leu Ala Asn Leu Met Trp Leu Ala Pro Glu Ser Val Asp Val Ser Thr
1970 1975 1980
Asn Gly Ser Pro His Lys Ile Asp Ile Val Pro Ala Pro Ala Ala Pro
1985 1990 1995 2000
Leu Ala Val Glu Val Pro Glu Leu Trp Ala Pro Ile Leu Asp Gly Phe
2005 2010 2015
Thr Ala Asp Glu Asp Glu Glu Ala Glu Gly Ala Leu Gln Ile Leu Ala
2020 2025 2030
Lys Glu His Ala Leu Val Pro Glu Thr Thr Gly Asp Glu Leu Ser Ser
2035 2040 2045
Ile Pro Thr Ile Ala Thr Trp Pro Ser Val Lys Ile Ala Leu Leu Tyr
2050 2055 2060
Glu Ser Asp Pro Asp Glu Pro Leu Glu Asp Asp Leu Lys Ala Glu Gly
2065 2070 2075 2080
Trp Thr Leu Leu Phe Ala Asn Asp Leu Glu Thr Ser Asp Ile Pro Ala
2085 2090 2095
Ala Leu Arg Pro
2100
<210>61
<211>4930
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(4900)
<223>RXA02363
<400>61
ttcctcgtct cacaggattt gacctggtga gtgccgaagc gaaagtgctc agcgcaataa 60
acaacaacaa tataatccca actgcaattt aaggatcact atg tcc aac gca cct 115
Met Ser Asn Ala Pro
1 5
aaa aag agc ttt tca gac ttc ttt tcc cca cta gca acc gaa act gaa 163
Lys Lys Ser Phe Ser Asp Phe Phe Ser Pro Leu Ala Thr Glu Thr Glu
10 15 20
aca act att acg ctc tcg gca att gag gta gag aaa gtc aat gce aag 211
Thr Thr Ile Thr Leu Ser Ala Ile Glu Val Glu Lys Val Asn Ala Lys
25 30 35
act gaa gtc act cga aat att gat cca gtc gaa gca gca gaa caa atc 259
Thr Glu Val Thr Arg Asn Ile Asp Pro Val Glu Ala Ala Glu Gln Ile
40 45 50
agc cgc gac tac cgc cgc tac ctg aaa aca ctt att agc ccc agt aat 307
Ser Arg Asp Tyr Arg Arg Tyr Leu Lys Thr Leu Ile Ser Pro Ser Asn
55 60 65
aag aca att gcg gca gaa ttc aac cga gag att gat gag tcc gaa aac 355
Lys Thr Ile Ala Ala Glu Phe Asn Arg Glu Ile Asp Glu Ser Glu Asn
70 75 80 85
ctt gtc tac gga cca att ttg cag ctg act ccg cct tat gca cca ggt 403
Leu Val Tyr Gly Pro Ile Leu Gln Leu Thr Pro Pro Tyr Ala Pro Gly
90 95 100
aag tcc cct gct caa tta att gat gag gga gtc ctt tct cca aac ttc 451
Lys Ser Pro Ala Gln Leu Ile Asp Glu Gly Val Leu Ser Pro Asn Phe
105 110 115
tca cgt ctc gat gcc gca cta ccc aaa gat cgc ccg ctt tat cag cat 499
Ser Arg Leu Asp Ala Ala Leu Pro Lys Asp Arg Pro Leu Tyr Gln His
120 125 130
cag gaa gac gct ctg cga aaa atc gct agt ggc cga aac tta atc gtt 547
Gln Glu Asp Ala Leu Arg Lys Ile Ala Ser Gly Arg Asn Leu Ile Val
135 140 145
tca acc ggt aca gga tct gga aaa aca gag tct ttc ctc atc ccg atc 595
Ser Thr Gly Thr Gly Ser Gly Lys Thr Glu Ser Phe Leu Ile Pro Ile
150 155 160 165
ttt gac caa ctg ctc cgc caa caa caa gcc ggt gag tta aat cct ggt 643
Phe Asp Gln Leu Leu Arg Gln Gln Gln Ala Gly Glu Leu Asn Pro Gly
170 175 180
gtc aga gca ttg ctt ctt tac cct atg aat gct ctt gca aat gat cag 691
Val Arg Ala Leu Leu Leu Tyr Pro Met Asn Ala Leu Ala Asn Asp Gln
185 190 195
gag aaa cgt ctc cgc gaa tta ttg gca gac acc ccg gaa att act ttt 739
Glu Lys Arg Leu Arg Glu Leu Leu Ala Asp Thr Pro Glu Ile Thr Phe
200 205 210
ggt cga tac acc gga gat acc aaa cag acc cgt gaa gaa gcc gag aaa 787
Gly Arg Tyr Thr Gly Asp Thr Lys Gln Thr Arg Glu Glu Ala Glu Lys
215 220 225
tac ttc aaa ctg att aat ggg cga aat gcg acc cct cta cct aat gag 835
Tyr Phe Lys Leu Ile Asn Gly Arg Asn Ala Thr Pro Leu Pro Asn Glu
230 235 240 245
cta att agc cgt gat gag atg cag gaa aat cca cct cat atc ttg ctc 883
Leu Ile Ser Arg Asp Glu Met Gln Glu Asn Pro Pro His Ile Leu Leu
250 255 260
acc aac tac gca atg ttg gaa tac ctc ctg ctt cgt cct gca gac aac 931
Thr Asn Tyr Ala Met Leu Glu Tyr Leu Leu Leu Arg Pro Ala Asp Asn
265 270 275
gca ttc ttc gat gat gca tat tcc aat aat tgg aag ttc ctt gtt ctt 979
Ala Phe Phe Asp Asp Ala Tyr Ser Asn Asn Trp Lys Phe Leu Val Leu
280 285 290
gat gaa gct cat gtc tac gcc gga gca caa ggc act gaa gtc ggc atg 1027
Asp Glu Ala His Val Tyr Ala Gly Ala Gln Gly Thr Glu Val Gly Met
295 300 305
ctg atg agg cgc ctg aaa gat cgt gtc caa cga gga aat ccc ctc cag 1075
Leu Met Arg Arg Leu Lys Asp Arg Val Gln Arg Gly Asn Pro Leu Gln
310 315 320 325
tgc atc gct aca agt gct tct ttg gaa gga act aaa gaa gcc atc atg 1123
Cys Ile Ala Thr Ser Ala Ser Leu Glu Gly Thr Lys Glu Ala Ile Met
330 335 340
acc ttt gga caa gac ctc ttt ggt gaa cct ttt gag ttt gtc aac gaa 1171
Thr Phe Gly Gln Asp Leu Phe Gly Glu Pro Phe Glu Phe Val Asn Glu
345 350 355
gat ccc tcg cgc cag gat ttg gta tcc gcg cat cgg aga aaa cta cca 1219
Asp Pro Ser Arg Gln Asp Leu Val Ser Ala His Arg Arg Lys Leu Pro
360 365 370
aaa acc ttt acg tgg tcc cta cca gat gag ctc ttt gac cag cct tta 1267
Lys Thr Phe Thr Trp Ser Leu Pro Asp Glu Leu Phe Asp Gln Pro Leu
375 380 385
gaa agt gat ggt ctt ttc caa gca cta caa gag cgc ggt gga gat caa 1315
Glu Ser Asp Gly Leu Phe Gln Ala Leu Gln Glu Arg Gly Gly Asp Gln
390 395 400 405
tac gaa gag ctc tcc aaa gaa gaa cac atc gta aaa ctc cgc gaa cta 1363
Tyr Glu Glu Leu Ser Lys Glu Glu His Ile Val Lys Leu Arg Glu Leu
410 415 420
tcc cag caa tca agc act cga gtt gaa gac atc ggc aag ggt ctt tgg 1411
Ser Gln Gln Ser Ser Thr Arg Val Glu Asp Ile Gly Lys Gly Leu Trp
425 430 435
cca aat gtt act gat aaa gca tca atg cac cgc aca cat atg ctg gtg 1459
Pro Asn Val Thr Asp Lys Ala Ser Met His Arg Thr His Met Leu Val
440 445 450
aat tta ggc agt ggc gtt cta tcc cat gat gga gta cca gct ctg tcc 1507
Asn Leu Gly Ser Gly Val Leu Ser His Asp Gly Val Pro Ala Leu Ser
455 460 465
gcg cgt tat cac atg ttt gtc cgc gca gtt gaa ggc gct ttt ctt ggg 1555
Ala Arg Tyr His Met Phe Val Arg Ala Val Glu Gly Ala Phe Leu Gly
470 475 480 485
tat aca gag caa gga aag cca atc gtg tcc ttg gac cgg caa gtg aca 1603
Tyr Thr Glu Gln Gly Lys Pro Ile Val Ser Leu Asp Arg Gln Val Thr
490 495 500
tta ggc gac aca gct cga cca atg tat gaa atg ggt gct tgc atc aaa 1651
Leu Gly Asp Thr Ala Arg Pro Met Tyr Glu Met Gly Ala Cys Ile Lys
505 510 515
tgt gga acc gtt cat atc agc gcc cat aat gat tct ggt ttc ctt gtg 1699
Cys Gly Thr Val His Ile Ser Ala His Asn Asp Ser Gly Phe Leu Val
520 525 530
cct cca gag aat tcc tca aat ttc gac gaa caa cag ctc aaa tgg gta 1747
Pro Pro Glu Asn Ser Ser Asn Phe Asp Glu Gln Gln Leu Lys Trp Val
535 540 545
gtc ctc acc gat gat ttc gaa aca gca gac atc gac gaa gat gac cta 1795
Val Leu Thr Asp Asp Phe Glu Thr Ala Asp Ile Asp Glu Asp Asp Leu
550 555 560 565
gaa act gat gct gac gag aac gtc aaa gtc ctt gag cta caa aaa tta 1843
Glu Thr Asp Ala Asp Glu Asn Val Lys Val Leu Glu Leu Gln Lys Leu
570 575 580
tgt acg gcc tgc gga aaa cta aac gga aag aac tct ctt ctc tgc tcc 1891
Cys Thr Ala Cys Gly Lys Leu Asn Gly Lys Asn Ser Leu Leu Cys Ser
585 590 595
gga tgc tca tca cat cat gac cag ttt att gat gta aag atc ttg gaa 1939
Gly Cys Ser Ser His His Asp Gln Phe Ile Asp Val Lys Ile Leu Glu
600 605 610
ccc cgg aat ggt agc caa tta aca tgc act cga tgc ggt ggc cga gaa 1987
Pro Arg Asn Gly Ser Gln Leu Thr Cys Thr Arg Cys Gly Gly Arg Glu
615 620 625
aag aac ctg att cga cga ctc cga acc gat tca aat gct gca cct tct 2035
Lys Asn Leu Ile Arg Arg Leu Arg Thr Asp Ser Asn Ala Ala Pro Ser
630 635 640 645
gtc ctc acc aca tca ttg ttc caa ctg ttg cca gaa tca gct gat caa 2083
Val Leu Thr Thr Ser Leu Phe Gln Leu Leu Pro Glu Ser Ala Asp Gln
650 655 660
gat aca tca cgc aag atc ggt gct gga cgc aaa cta ctc acg ttt tct 2131
Asp Thr Ser Arg Lys Ile Gly Ala Gly Arg Lys Leu Leu Thr Phe Ser
665 670 675
gac tcc cgc cag gct gcc gct tac gca gca cca tat ttg caa gca agt 2179
Asp Ser Arg Gln Ala Ala Ala Tyr Ala Ala Pro Tyr Leu Gln Ala Ser
680 685 690
tac acc agg ttg cta gag cga aga atc ctc att gag acc ctg cgg gat 2227
Tyr Thr Arg Leu Leu Glu Arg Arg Ile Leu Ile Glu Thr Leu Arg Asp
695 700 705
gaa gaa ttt act gaa ggc gca tcc att gaa cga tgg att tcc cgc gca 2275
Glu Glu Phe Thr Glu Gly Ala Ser Ile Glu Arg Trp Ile Ser Arg Ala
710 715 720 725
agt gaa gtg gcg aaa aac aat cga gtc ctc gct aac aac ttg aac cca 2323
Ser Glu Val Ala Lys Asn Asn Arg Val Leu Ala Asn Asn Leu Asn Pro
730 735 740
cgc gaa aca cta gaa cag aca ggt aac tgg gta ttc gca gat ctt gct 2371
Arg Glu Thr Leu Glu Gln Thr Gly Asn Trp Val Phe Ala Asp Leu Ala
745 750 755
tcg aca gtc cga cgt agt tcg act gaa ggg ctt gga cta gcc aaa att 2419
Ser Thr Val Arg Arg Ser Ser Thr Glu Gly Leu Gly Leu Ala Lys Ile
760 765 770
gaa ctg acc cct gag gct ttg tcg caa ctc agt ttc aga aaa cca ctt 2467
Glu Leu Thr Pro Glu Ala Leu Ser Gln Leu Ser Phe Arg Lys Pro Leu
775 780 785
ggc gag atg ttt ggg gat ccg gat gcc gcc gac gcc ttt ttt aat ctc 2515
Gly Glu Met Phe Gly Asp Pro Asp Ala Ala Asp Ala Phe Phe Asn Leu
790 795 800 805
ttc gct caa gaa ttc cgt cac aag ggc gca atc aat tgc cct gac tac 2563
Phe Ala Gln Glu Phe Arg His Lys Gly Ala Ile Asn Cys Pro Asp Tyr
810 815 820
gtc aat ctt gag gat gaa cgc ttt ggc cct cgt cgt ggg cag cac ttt 2611
Val Asn Leu Glu Asp Glu Arg Phe Gly Pro Arg Arg Gly Gln His Phe
825 830 835
ttc acc aaa gat ggt gga aga aaa tca aca cga cgc ctc tac tca tgg 2659
Phe Thr Lys Asp Gly Gly Arg Lys Ser Thr Arg Arg Leu Tyr Ser Trp
840 845 850
att ccg caa cga gga acc aac aat cgc aag gac ttc att aca aaa gtc 2707
Ile Pro Gln Arg Gly Thr Asn Asn Arg Lys Asp Phe Ile Thr Lys Val
855 860 865
ttg aac cgc atc gga caa gca ggt gac gaa ggt gaa aat att acg acg 2755
Leu Asn Arg Ile Gly Gln Ala Gly Asp Glu Gly Glu Asn Ile Thr Thr
870 875 880 885
ctt ctc cat cac cta tgg aat gac tac act aat tcc gaa att ttg aag 2803
Leu Leu His His Leu Trp Asn Asp Tyr Thr Asn Ser Glu Ile Leu Lys
890 895 900
gtc cca ggc gaa aag gcc gaa ggc tac aca ctt aac tac aac tca ctg 2851
Val Pro Gly Glu Lys Ala Glu Gly Tyr Thr Leu Asn Tyr Asn Ser Leu
905 910 915
caa gta agc cct gga aaa caa cat tcc tgg tat gaa tgt gac act tgt 2899
Gln Val Ser Pro Gly Lys Gln His Ser Trp Tyr Glu Cys Asp Thr Cys
920 925 930
aga aat aca acg cca ttt aat gtt ctc ggg ctg tgt cca cat ggg ttc 2947
Arg Asn Thr Thr Pro Phe Asn Val Leu Gly Leu Cys Pro His Gly Phe
935 940 945
tgt aaa ggc aag cta aaa gaa atc gat act tct tta ccg gag tat gcc 2995
Cys Lys Gly Lys Leu Lys Glu Ile Asp Thr Ser Leu Pro Glu Tyr Ala
950 955 960 965
aac aat cat tat cgg aag ttg gca aca tcg ctt gaa att ctt ccg ctt 3043
Asn Asn His Tyr Arg Lys Leu Ala Thr Ser Leu Glu Ile Leu Pro Leu
970 975 980
tca gcc aaa gag cac act gct caa tgg acg cca acg gag gct gca gag 3091
Ser Ala Lys Glu His Thr Ala Gln Trp Thr Pro Thr Glu Ala Ala Glu
985 990 995
gtt cag aaa gaa ttc atc gaa gga aaa atc aat gtc cta agc tgt tct 3139
Val Gln Lys Glu Phe Ile Glu Gly Lys Ile Asn Val Leu Ser Cys Ser
1000 1005 1010
act acg ttt gag ctt gga gtt gac gtt ggt gat ctg cag tca gta atg 3187
Thr Thr Phe Glu Leu Gly Val Asp Val Gly Asp Leu Gln Ser Val Met
1015 1020 1025
atg cgc aac gta ccg cca cgt acg gct aac tat gtg caa cgt gcg gga 3235
Met Arg Asn Val Pro Pro Arg Thr Ala Asn Tyr Val Gln Arg Ala Gly
1030 1035 1040 1045
cgt gcg gga cgc cga tca ggt tca gca gct ttt gtg ctt acc ttt gct 3283
Arg Ala Gly Arg Arg Ser Gly Ser Ala Ala Phe Val Leu Thr Phe Ala
1050 1055 1060
aag cga tct tct cat gac ttg gct gta ttt aag aac cca acc cag atg 3331
Lys Arg Ser Ser His Asp Leu Ala Val Phe Lys Asn Pro Thr Gln Met
1065 1070 1075
att gat ggc gaa atg aca gtg cca ttt ttg cac att aac aat gcc cga 3379
Ile Asp Gly Glu Met Thr Val Pro Phe Leu His Ile Asn Asn Ala Arg
1080 1085 1090
atc gcc cgt cgt cac acc tat tca atc gct ctc gcc gca ttt ttc cga 3427
Ile Ala Arg Arg His Thr Tyr Ser Ile Ala Leu Ala Ala Phe Phe Arg
1095 1100 1105
gag cag gct gcc caa aat cgg ttc tgg aaa aag gcc gga gag ttc ttc 3475
Glu Gln Ala Ala Gln Asn Arg Phe Trp Lys Lys Ala Gly Glu Phe Phe
1110 1115 1120 1125
ctt ggc act gat gca gct cca tac ctt agg cct gct gtc gca gag caa 3523
Leu Gly Thr Asp Ala Ala Pro Tyr Leu Arg Pro Ala Val Ala Glu Gln
1130 1135 1140
gag gca acg gag atc ctc gaa gag ttt ctc tcc cct gtt cct aat tac 3571
Glu Ala Thr Glu Ile Leu Glu Glu Phe Leu Ser Pro Val Pro Asn Tyr
1145 1150 1155
atc act gaa gcc ctc cgt cgc gta ttc cct gag tcc ctg cat gag gat 3619
Ile Thr Glu Ala Leu Arg Arg Val Phe Pro Glu Ser Leu His Glu Asp
1160 1165 1170
ctg gat att gaa aat caa ggt tgg gtt aaa caa ttc ctt gaa att ttc 3667
Leu Asp Ile Glu Asn Gln Gly Trp Val Lys Gln Phe Leu Glu Ile Phe
1175 1180 1185
gat aca act cgt cag gag atc tcc gaa gat ttc caa acg ctt aaa aag 3715
Asp Thr Thr Arg Gln Glu Ile Ser Glu Asp Phe Gln Thr Leu Lys Lys
1190 1195 1200 1205
atg caa ggg cga gcg ctg ggc agt gaa caa ggc aaa aag gca gat gcc 3763
Met Gln Gly Arg Ala Leu Gly Ser Glu Gln Gly Lys Lys Ala Asp Ala
1210 1215 1220
ttc aaa cgc aca atc aca act ctg atg gac caa gat ctt ttg gga tac 3811
Phe Lys Arg Thr Ile Thr Thr Leu Met Asp Gln Asp Leu Leu Gly Tyr
1225 1230 1235
ttg gcc aag aag aac atg ctg ccc aag tac agc ttc cct gtc gat aca 3859
Leu Ala Lys Lys Asn Met Leu Pro Lys Tyr Ser Phe Pro Val Asp Thr
1240 1245 1250
gta gat ctt caa aca aac ttc agt gaa gct gga aat aag gtg agc ttg 3907
Val Asp Leu Gln Thr Asn Phe Ser Glu Ala Gly Asn Lys Val Ser Leu
1255 1260 1265
tca cga gat ctt cag cta gcg att acc gac tat gct cct ggt gcg gag 3955
Ser Arg Asp Leu Gln Leu Ala Ile Thr Asp Tyr Ala Pro Gly Ala Glu
1270 1275 1280 1285
tta gta gca ggc gga aaa ttg tgg aaa tcc gct ggc att cga cac cta 4003
Leu Val Ala Gly Gly Lys Leu Trp Lys Ser Ala Gly Ile Arg His Leu
1290 1295 1300
gcc ggt aaa aag gtg gaa act ttt tat tgg aca acg tgt act gag tgt 4051
Ala Gly Lys Lys Val Glu Thr Phe Tyr Trp Thr Thr Cys Thr Glu Cys
1305 1310 1315
aaa cat act gaa acc tct cgt ttt gga ttt aca tct gaa gat gtc tgt 4099
Lys His Thr Glu Thr Ser Arg Phe Gly Phe Thr Ser Glu Asp Val Cys
1320 1325 1330
tct cag tgc tct gca cca ata tcg ctg ggt aaa gag aac aag ttc ctt 4147
Ser Gln Cys Ser Ala Pro Ile Ser Leu Gly Lys Glu Asn Lys Phe Leu
1335 1340 1345
atc cct cgt ttt ggt ttc gtt gca gac cca aat cca act gaa gtg ggc 4195
Ile Pro Arg Phe Gly Phe Val Ala Asp Pro Asn Pro Thr Glu Val Gly
1350 1355 1360 1365
act gct ccc ccg gtc cga tcc tct aat cga ctt gaa ttt gta aag cag 4243
Thr Ala Pro Pro Val Arg Ser Ser Asn Arg Leu Glu Phe Val Lys Gln
1370 1375 1380
ttc ggt gtc aaa gat gac agt gaa gag ttc tca aat tca gat ggg aca 4291
Phe Gly Val Lys Asp Asp Ser Glu Glu Phe Ser Asn Ser Asp Gly Thr
1385 1390 1395
gca act gcg caa gta ctt act tct tct tgg agt cgg acg gag atg gga 4339
Ala Thr Ala Gln Val Leu Thr Ser Ser Trp Ser Arg Thr Glu Met Gly
1400 1405 1410
gcg ctt gaa aca ggt cct aac aag aat gga ttt tgg tat tgc caa aca 4387
Ala Leu Glu Thr Gly Pro Asn Lys Asn Gly Phe Trp Tyr Cys Gln Thr
1415 1420 1425
tgc gga ttc ggc act ccc aat ggc gcc gaa atc cct aaa agc cat cga 4435
Cys Gly Phe Gly Thr Pro Asn Gly Ala Glu Ile Pro Lys Ser His Arg
1430 1435 1440 1445
aac ccc aga act aag cag cag tgt ggc acc tac tac tta gaa ccc cac 4483
Asn Pro Arg Thr Lys Gln Gln Cys Gly Thr Tyr Tyr Leu Glu Pro His
1450 1455 1460
tct ttg ggt cac acc tac caa act gac atc gcg aca gtt gcc gtt cct 4531
Ser Leu Gly His Thr Tyr Gln Thr Asp Ile Ala Thr Val Ala Val Pro
1465 1470 1475
tct tat aca aat ctt gac ttc gaa ggg tgg cgc tct gga atg tac gcc 4579
Ser Tyr Thr Asn Leu Asp Phe Glu Gly Trp Arg Ser Gly Met Tyr Ala
1480 1485 1490
atc atc gag gca gct gct gaa tgt tta gaa atc aat cgt gat gat cta 4627
Ile Ile Glu Ala Ala Ala Glu Cys Leu Glu Ile Asn Arg Asp Asp Leu
1495 1500 1505
aac ggc acc atg gcg aaa cac gac aac agg cca acg atg gtg ttg ttc 4675
Asn Gly Thr Met Ala Lys His Asp Asn Arg Pro Thr Met Val Leu Phe
1510 1515 1520 1525
gat act gtt cct ggt gga gct ggt att acc cga aaa gtt cga gaa aac 4723
Asp Thr Val Pro Gly Gly Ala Gly Ile Thr Arg Lys Val Arg Glu Asn
1530 1535 1540
ttc cct caa gta ctc gaa gcg gca att aga agg gtc gaa acc tgt tct 4771
Phe Pro Gln Val Leu Glu Ala Ala Ile Arg Arg Val Glu Thr Cys Ser
1545 1550 1555
tgt ggc atc gat act tcg tgt tat gcc tgt ttg cga tca ttt tct aac 4819
Cys Gly Ile Asp Thr Ser Cys Tyr Ala Cys Leu Arg Ser Phe Ser Asn
1560 1565 1570
cag cgt ttc cat ctc gat tta agg cgc gat att gca ttg gac ttg ctt 4867
Gln Arg Phe His Leu Asp Leu Arg Arg Asp Ile Ala Leu Asp Leu Leu
1575 1580 1585
cac cat atg gcg gtt gcg atg cca aaatct gag tagataacac cagctaccca 4920
His His Met Ala Val Ala Met Pro Lys Ser Glu
1590 1595 1600
cagctaaaag 4930
<210>62
<211>1600
<212>PRT
<213>谷氨酸棒状杆菌
<400>62
Met Ser Asn Ala Pro Lys Lys Ser Phe Ser Asp Phe Phe Ser Pro Leu
1 5 10 15
Ala Thr Glu Thr Glu Thr Thr Ile Thr Leu Ser Ala Ile Glu Val Glu
20 25 30
Lys Val Asn Ala Lys Thr Glu Val Thr Arg Asn Ile Asp Pro Val Glu
35 40 45
Ala Ala Glu Gln Ile Ser Arg Asp Tyr Arg Arg Tyr Leu Lys Thr Leu
50 55 60
Ile Ser Pro Ser Asn Lys Thr Ile Ala Ala Glu Phe Asn Arg Glu Ile
65 70 75 80
Asp Glu Ser Glu Asn Leu Val Tyr Gly Pro Ile Leu Gln Leu Thr Pro
85 90 95
Pro Tyr Ala Pro Gly Lys Ser Pro Ala Gln Leu Ile Asp Glu Gly Val
100 105 110
Leu Ser Pro Asn Phe Ser Arg Leu Asp Ala Ala Leu Pro Lys Asp Arg
115 120 125
Pro Leu Tyr Gln His Gln Glu Asp Ala Leu Arg Lys Ile Ala Ser Gly
130 135 140
Arg Asn Leu Ile Val Ser Thr Gly Thr Gly Ser Gly Lys Thr Glu Ser
145 150 155 160
Phe Leu Ile Pro Ile Phe Asp Gln Leu Leu Arg Gln Gln Gln Ala Gly
165 170 175
Glu Leu Asn Pro Gly Val Arg Ala Leu Leu Leu Tyr Pro Met Asn Ala
180 185 190
Leu Ala Asn Asp Gln Glu Lys Arg Leu Arg Glu Leu Leu Ala Asp Thr
195 200 205
Pro Glu Ile Thr Phe Gly Arg Tyr Thr Gly Asp Thr Lys Gln Thr Arg
210 215 220
Glu Glu Ala Glu Lys Tyr Phe Lys Leu Ile Asn Gly Arg Asn Ala Thr
225 230 235 240
Pro Leu Pro Asn Glu Leu Ile Ser Arg Asp Glu Met Gln Glu Asn Pro
245 250 255
Pro His Ile Leu Leu Thr Asn Tyr Ala Met Leu Glu Tyr Leu Leu Leu
260 265 270
Arg Pro Ala Asp Asn Ala Phe Phe Asp Asp Ala Tyr Ser Asn Asn Trp
275 280 285
Lys Phe Leu Val Leu Asp Glu Ala His Val Tyr Ala Gly Ala Gln Gly
290 295 300
Thr Glu Val Gly Met Leu Met Arg Arg Leu Lys Asp Arg Val Gln Arg
305 310 315 320
Gly Asn Pro Leu Gln Cys Ile Ala Thr Ser Ala Ser Leu Glu Gly Thr
325 330 335
Lys Glu Ala Ile Met Thr Phe Gly Gln Asp Leu Phe Gly Glu Pro Phe
340 345 350
Glu Phe Val Asn Glu Asp Pro Ser Arg Gln Asp Leu Val Ser Ala His
355 360 365
Arg Arg Lys Leu Pro Lys Thr Phe Thr Trp Ser Leu Pro Asp Glu Leu
370 375 380
Phe Asp Gln Pro Leu Glu Ser Asp Gly Leu Phe Gln Ala Leu Gln Glu
385 390 395 400
Arg Gly Gly Asp Gln Tyr Glu Glu Leu Ser Lys Glu Glu His Ile Val
405 410 415
Lys Leu Arg Glu Leu Ser Gln Gln Ser Ser Thr Arg Val Glu Asp Ile
420 425 430
Gly Lys Gly Leu Trp Pro Asn Val Thr Asp Lys Ala Ser Met His Arg
435 440 445
Thr His Met Leu Val Asn Leu Gly Ser Gly Val Leu Ser His Asp Gly
450 455 460
Val Pro Ala Leu Ser Ala Arg Tyr His Met Phe Val Arg Ala Val Glu
465 470 475 480
Gly Ala Phe Leu Gly Tyr Thr Glu Gln Gly Lys Pro Ile Val Ser Leu
485 490 495
Asp Arg Gln Val Thr Leu Gly Asp Thr Ala Arg Pro Met Tyr Glu Met
500 505 510
Gly Ala Cys Ile Lys Cys Gly Thr Val His Ile Ser Ala His Asn Asp
515 520 525
Ser Gly Phe Leu Val Pro Pro Glu Asn Ser Ser Asn Phe Asp Glu Gln
530 535 540
Gln Leu Lys Trp Val Val Leu Thr Asp Asp Phe Glu Thr Ala Asp Ile
545 550 555 560
Asp Glu Asp Asp Leu Glu Thr Asp Ala Asp Glu Asn Val Lys Val Leu
565 570 575
Glu Leu Gln Lys Leu Cys Thr Ala Cys Gly Lys Leu Asn Gly Lys Asn
580 585 590
Ser Leu Leu Cys Ser Gly Cys Ser Ser His His Asp Gln Phe Ile Asp
595 600 605
Val Lys Ile Leu Glu Pro Arg Asn Gly Ser Gln Leu Thr Cys Thr Arg
610 615 620
Cys Gly Gly Arg Glu Lys Asn Leu Ile Arg Arg Leu Arg Thr Asp Ser
625 630 635 640
Asn Ala Ala Pro Ser Val Leu Thr Thr Ser Leu Phe Gln Leu Leu Pro
645 650 655
Glu Ser Ala Asp Gln Asp Thr Ser Arg Lys Ile Gly Ala Gly Arg Lys
660 665 670
Leu Leu Thr Phe Ser Asp Ser Arg Gln Ala Ala Ala Tyr Ala Ala Pro
675 680 685
Tyr Leu Gln Ala Ser Tyr Thr Arg Leu Leu Glu Arg Arg Ile Leu Ile
690 695 700
Glu Thr Leu Arg Asp Glu Glu Phe Thr Glu Gly Ala Ser Ile Glu Arg
705 710 715 720
Trp Ile Ser Arg Ala Ser Glu Val Ala Lys Asn Asn Arg Val Leu Ala
725 730 735
Asn Asn Leu Asn Pro Arg Glu Thr Leu Glu Gln Thr Gly Asn Trp Val
740 745 750
Phe Ala Asp Leu Ala Ser Thr Val Arg Arg Ser Ser Thr Glu Gly Leu
755 760 765
Gly Leu Ala Lys Ile Glu Leu Thr Pro Glu Ala Leu Ser Gln Leu Ser
770 775 780
Phe Arg Lys Pro Leu Gly Glu Met Phe Gly Asp Pro Asp Ala Ala Asp
785 790 795 800
Ala Phe Phe Asn Leu Phe Ala Gln Glu Phe Arg His Lys Gly Ala Ile
805 810 815
Asn Cys Pro Asp Tyr Val Asn Leu Glu Asp Glu Arg Phe Gly Pro Arg
820 825 830
Arg Gly Gln His Phe Phe Thr Lys Asp Gly Gly Arg Lys Ser Thr Arg
835 840 845
Arg Leu Tyr Ser Trp Ile Pro Gln Arg Gly Thr Asn Asn Arg Lys Asp
850 855 860
Phe Ile Thr Lys Val Leu Asn Arg Ile Gly Gln Ala Gly Asp Glu Gly
865 870 875 880
Glu Asn Ile Thr Thr Leu Leu His His Leu Trp Asn Asp Tyr Thr Asn
885 890 895
Ser Glu Ile Leu Lys Val Pro Gly Glu Lys Ala Glu Gly Tyr Thr Leu
900 905 910
Asn Tyr Asn Ser Leu Gln Val Ser Pro Gly Lys Gln His Ser Trp Tyr
915 920 925
Glu Cys Asp Thr Cys Arg Asn Thr Thr Pro Phe Asn Val Leu Gly Leu
930 935 940
Cys Pro His Gly Phe Cys Lys Gly Lys Leu Lys Glu Ile Asp Thr Ser
945 950 955 960
Leu Pro Glu Tyr Ala Asn Asn His Tyr Arg Lys Leu Ala Thr Ser Leu
965 970 975
Glu Ile Leu Pro Leu Ser Ala Lys Glu His Thr Ala Gln Trp Thr Pro
980 985 990
Thr Glu Ala Ala Glu Val Gln Lys Glu Phe Ile Glu Gly Lys Ile Asn
995 1000 1005
Val Leu Ser Cys Ser Thr Thr Phe Glu Leu Gly Val Asp Val Gly Asp
1010 1015 1020
Leu Gln Ser Val Met Met Arg Asn Val Pro Pro Arg Thr Ala Asn Tyr
1025 1030 1035 1040
Val Gln Arg Ala Gly Arg Ala Gly Arg Arg Ser Gly Ser Ala Ala Phe
1045 1050 1055
Val Leu Thr Phe Ala Lys Arg Ser Ser His Asp Leu Ala Val Phe Lys
1060 1065 1070
Asn Pro Thr Gln Met Ile Asp Gly Glu Met Thr Val Pro Phe Leu His
1075 1080 1085
Ile Asn Asn Ala Arg Ile Ala Arg Arg His Thr Tyr Ser Ile Ala Leu
1090 1095 1100
Ala Ala Phe Phe Arg Glu Gln Ala Ala Gln Asn Arg Phe Trp Lys Lys
1105 1110 1115 1120
Ala Gly Glu Phe Phe Leu Gly Thr Asp Ala Ala Pro Tyr Leu Arg Pro
1125 1130 1135
Ala Val Ala Glu Gln Glu Ala Thr Glu Ile Leu Glu Glu Phe Leu Ser
1140 1145 1150
Pro Val Pro Asn Tyr Ile Thr Glu Ala Leu Arg Arg Val Phe Pro Glu
1155 1160 1165
Ser Leu His Glu Asp Leu Asp Ile Glu Asn Gln Gly Trp Val Lys Gln
1170 1175 1180
Phe Leu Glu Ile Phe Asp Thr Thr Arg Gln Glu Ile Ser Glu Asp Phe
1185 1190 1195 1200
Gln Thr Leu Lys Lys Met Gln Gly Arg Ala Leu Gly Ser Glu Gln Gly
1205 1210 1215
Lys Lys Ala Asp Ala Phe Lys Arg Thr Ile Thr Thr Leu Met Asp Gln
1220 1225 1230
Asp Leu Leu Gly Tyr Leu Ala Lys Lys Asn Met Leu Pro Lys Tyr Ser
1235 1240 1245
Phe Pro Val Asp Thr Val Asp Leu Gln Thr Asn Phe Ser Glu Ala Gly
1250 1255 1260
Asn Lys Val Ser Leu Ser Arg Asp Leu Gln Leu Ala Ile Thr Asp Tyr
1265 1270 1275 1280
Ala Pro Gly Ala Glu Leu Val Ala Gly Gly Lys Leu Trp Lys Ser Ala
1285 1290 1295
Gly Ile Arg His Leu Ala Gly Lys Lys Val Glu Thr Phe Tyr Trp Thr
1300 1305 1310
Thr Cys Thr Glu Cys Lys His Thr Glu Thr Ser Arg Phe Gly Phe Thr
1315 1320 1325
Ser Glu Asp Val Cys Ser Gln Cys Ser Ala Pro Ile Ser Leu Gly Lys
1330 1335 1340
Glu Asn Lys Phe Leu Ile Pro Arg Phe Gly Phe Val Ala Asp Pro Asn
1345 1350 1355 1360
Pro Thr Glu Val Gly Thr Ala Pro Pro Val Arg Ser Ser Asn Arg Leu
1365 1370 1375
Glu Phe Val Lys Gln Phe Gly Val Lys Asp Asp Ser Glu Glu Phe Ser
1380 1385 1390
Asn Ser Asp Gly Thr Ala Thr Ala Gln Val Leu Thr Ser Ser Trp Ser
1395 1400 1405
Arg Thr Glu Met Gly Ala Leu Glu Thr Gly Pro Asn Lys Asn Gly Phe
1410 1415 1420
Trp Tyr Cys Gln Thr Cys Gly Phe Gly Thr Pro Asn Gly Ala Glu Ile
1425 1430 1435 1440
Pro Lys Ser His Arg Asn Pro Arg Thr Lys Gln Gln Cys Gly Thr Tyr
1445 1450 1455
Tyr Leu Glu Pro His Ser Leu Gly His Thr Tyr Gln Thr Asp Ile Ala
1460 1465 1470
Thr Val Ala Val Pro Ser Tyr Thr Asn Leu Asp Phe Glu Gly Trp Arg
1475 1480 1485
Ser Gly Met Tyr Ala Ile Ile Glu Ala Ala Ala Glu Cys Leu Glu Ile
1490 1495 1500
Asn Arg Asp Asp Leu Asn Gly Thr Met Ala Lys His Asp Asn Arg Pro
1505 1510 1515 1520
Thr Met Val Leu Phe Asp Thr Val Pro Gly Gly Ala Gly Ile Thr Arg
1525 1530 1535
Lys Val Arg Glu Asn Phe Pro Gln Val Leu Glu Ala Ala Ile Arg Arg
1540 1545 1550
Val Glu Thr Cys Ser Cys Gly Ile Asp Thr Ser Cys Tyr Ala Cys Leu
1555 1560 1565
Arg Ser Phe Ser Asn Gln Arg Phe His Leu Asp Leu Arg Arg Asp Ile
1570 1575 1580
Ala Leu Asp Leu Leu His His Met Ala Val Ala Met Pro Lys Ser Glu
1585 1590 1595 1600
<210>63
<211>697
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(667)
<223>RXA02533
<400>63
gttttgtgca atccaatgct actcccacag agcgggctac tttctctaaa aatgttctca 60
tagtagataa aattgttctt aaagcgacat tattgtctgc atg gaa gac gat ctc 115
Met Glu Asp Asp Leu
1 5
agt gct gct ctc gtc aaa gcg ctt ttc gac gcg cga acc caa cgc agg 163
Ser Ala Ala Leu Val Lys Ala Leu Phe Asp Ala Arg Thr Gln Arg Arg
10 15 20
ctc tct atc tcg gcg tta gct gaa tcc tcc ggt gtg tcg cga gca atg 211
Leu Ser Ile Ser Ala Leu Ala Glu Ser Ser Gly Val Ser Arg Ala Met
25 30 35
att tcc cgc gtg gaa aac gca gag gcg caa cca agc gct gca tta ctt 259
Ile Ser Arg Val Glu Asn Ala Glu Ala Gln Pro Ser Ala Ala Leu Leu
40 45 50
gga cgc ctt tcc ggt gca ttg ggt atg acg ctt tcg gag ctc att gca 307
Gly Arg Leu Ser Gly Ala Leu Gly Met Thr Leu Ser Glu Leu Ile Ala
55 60 65
cag gct gaa ggt ggc tat gac cgg ggc gct cgg cgg tca aag cag tct 355
Gln Ala Glu Gly Gly Tyr Asp Arg Gly Ala Arg Arg Ser Lys Gln Ser
70 75 80 85
gta tgg aca gat cca gct acc ggt tac aca cgg cgt gca gtg tca cag 403
Val Trp Thr Asp Pro Ala Thr Gly Tyr Thr Arg Arg Ala Val Ser Gln
90 95 100
ccg tca gaa tcc cca cta gaa cta gtg gaa gta atg ctg cct cct ggg 451
Pro Ser Glu Ser Pro Leu Glu Leu Val Glu Val Met Leu Pro Pro Gly
105 110 115
gcg gaa gtt ggc tac cca gct gat gct tat cgt ttc atg gat cag gtg 499
Ala Glu Val Gly Tyr Pro Ala Asp Ala Tyr Arg Phe Met Asp Gln Val
120 125 130
gtc tgg gta ctc gaa ggg gcc gtt cgt att act gaa ggt gaa gag gtc 547
Val Trp Val Leu Glu Gly Ala Val Arg Ile Thr Glu Gly Glu Glu Val
135 140 145
cac gaa ctt tca acg ggg gat tgt cta cgg ttt ggg cct ccg cga gat 595
His Glu Leu Ser Thr Gly Asp Cys Leu Arg Phe Gly Pro Pro Arg Asp
150 155 160 165
acc gac ttt gct aat ccc acc acc gta gcc act agg tat tta gtt gcc 643
Thr Asp Phe Ala Asn Pro Thr Thr Val Ala Thr Arg Tyr Leu Val Ala
170 175 180
ttg gac aag cgt gta cct cgt gct tgatataaca agtaaggaag cctgatgaat 697
Leu Asp Lys Arg Val Pro Arg Ala
185
<210>64
<211>189
<212>PRT
<213>谷氨酸棒状杆菌
<400>64
Met Glu Asp Asp Leu Ser Ala Ala Leu Val Lys Ala Leu Phe Asp Ala
1 5 10 15
Arg Thr Gln Arg Arg Leu Ser Ile Ser Ala Leu Ala Glu Ser Ser Gly
20 25 30
Val Ser Arg Ala Met Ile Ser Arg Val Glu Asn Ala Glu Ala Gln Pro
35 40 45
Ser Ala Ala Leu Leu Gly Arg Leu Ser Gly Ala Leu Gly Met Thr Leu
50 55 60
Ser Glu Leu Ile Ala Gln Ala Glu Gly Gly Tyr Asp Arg Gly Ala Arg
65 70 75 80
Arg Ser Lys Gln Ser Val Trp Thr Asp Pro Ala Thr Gly Tyr Thr Arg
85 90 95
Arg Ala Val Ser Gln Pro Ser Glu Ser Pro Leu Glu Leu Val Glu Val
100 105 110
Met Leu Pro Pro Gly Ala Glu Val Gly Tyr Pro Ala Asp Ala Tyr Arg
115 120 125
Phe Met Asp Gln Val Val Trp Val Leu Glu Gly Ala Val Arg Ile Thr
130 135 140
Glu Gly Glu Glu Val His Glu Leu Ser Thr Gly Asp Cys Leu Arg Phe
145 150 155 160
Gly Pro Pro Arg Asp Thr Asp Phe Ala Asn Pro Thr Thr Val Ala Thr
165 170 175
Arg Tyr Leu Val Ala Leu Asp Lys Arg Val Pro Arg Ala
180 185
<210>65
<211>3712
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3682)
<223>RXA02657
<400>65
tatgcgagta cgccgaaaat catagctgtt accttatacc taggttagaa aagccgagat 60
caatgcgtta atgaaatagg agtttcacgg gtgtaaggag atg gct aga ctg tcc 115
Met Ala Arg Leu Ser
1 5
cac atg gcc aag caa tcc tca ttt gta cat ctt cac aac cac acc gag 163
His Met Ala Lys Gln Ser Ser Phe Val His Leu His Asn His Thr Glu
10 15 20
ttt tcc atg ctt gat gga atg gcc aag atc gat atg ttg gcc gat gag 211
Phe Ser Met Leu Asp Gly Met Ala Lys Ile Asp Met Leu Ala Asp Glu
25 30 35
gtt aaa gcc cag gga atg cct gcg gtc gga atc acc gac cac ggc aat 259
Val Lys Ala Gln Gly Met Pro Ala Val Gly Ile Thr Asp His Gly Asn
40 45 50
atg tat ggc tcc aac ccc ttt tat cgc aag atg aca gag atg ggc att 307
Met Tyr Gly Ser Asn Pro Phe Tyr Arg Lys Met Thr Glu Met Gly Ile
55 60 65
aag ccc atc att ggc att gaa acg tat atg gca cct gag tct cgt ttt 355
Lys Pro Ile Ile Gly Ile Glu Thr Tyr Met Ala Pro Glu Ser Arg Phe
70 75 80 85
aag aaa gag cgt gtg cgt tgg ggc gaa cca cac caa aaa tca gat gat 403
Lys Lys Glu Arg Val Arg Trp Gly Glu Pro His Gln Lys Ser Asp Asp
90 95 100
gtt tct ggt tcc ggt gcg tat ttg cac cag acg atg ctt gca gaa aac 451
Val Ser Gly Ser Gly Ala Tyr Leu His Gln Thr Met Leu Ala Glu Asn
105 110 115
acc aca ggt tta aga aac ctc ttt tat cta tct tcg atg gca tcg tac 499
Thr Thr Gly Leu Arg Asn Leu Phe Tyr Leu Ser Ser Met Ala Ser Tyr
120 125 130
gaa ggc cag cta ggc aag tgg ccc cgc atg gac gcc gat atc atc gct 547
Glu Gly Gln Leu Gly Lys Trp Pro Arg Met Asp Ala Asp Ile Ile Ala
135 140 145
gag cac gcc gaa ggc atc atc gcc acc acg ggt tgc cct tcc ggc gat 595
Glu His Ala Glu Gly Ile Ile Ala Thr Thr Gly Cys Pro Ser Gly Asp
150 155 160 165
gtg caa aca cgc ctg cgc ttg ggc cag ttc gac gaa gcc ctt gaa gca 643
Val Gln Thr Arg Leu Arg Leu Gly Gln Phe Asp Glu Ala Leu Glu Ala
170 175 180
gcc gcc atg tgg cag gac atc tat ggt cgc gac aac tac ttc ctc gag 691
Ala Ala Met Trp Gln Asp Ile Tyr Gly Arg Asp Asn Tyr Phe Leu Glu
185 190 195
ttg atg gac cac ggg ctc gac att gaa acc cgt gtg cgc agt gag ctg 739
Leu Met Asp His Gly Leu Asp Ile Glu Thr Arg Val Arg Ser Glu Leu
200 205 210
ctc gaa atc gga cgc aag ctc aat ttg cca ccc ctg gtc acc aac gac 787
Leu Glu Ile Gly Arg Lys Leu Asn Leu Pro Pro Leu Val Thr Asn Asp
215 220 225
tgc cac tat gtg ctg gaa tct cag gcg caa gcc cac gag gca atg ctc 835
Cys His Tyr Val Leu Glu Ser Gln Ala Gln Ala His Glu Ala Met Leu
230 235 240 245
tgc gtg caa aca ggc aag acg ctt cat gat gaa gac cga ttc aaa ttc 883
Cys Val Gln Thr Gly Lys Thr Leu His Asp Glu Asp Arg Phe Lys Phe
250 255 260
ggc gga acc ggc tat tac gtt aaa tct gca gaa caa atg cgt gca ctc 931
Gly Gly Thr Gly Tyr Tyr Val Lys Ser Ala Glu Gln Met Arg Ala Leu
265 270 275
tgg gac gac atg gtt cca gat ggc tgc gac aac acc ctg tgg atc gct 979
Trp Asp Asp Met Val Pro Asp Gly Cys Asp Asn Thr Leu Trp Ile Ala
280 285 290
gaa cgt gtg cag tcc tat gac gaa atc tgg gaa gaa cac tca cac gac 1027
Glu Arg Val Gln Ser Tyr Asp Glu Ile Trp Glu Glu His Ser His Asp
295 300 305
cgc atg cct atc gct gat gtt cca gaa ggc tac acc cca acc act tgg 1075
Arg Met Pro Ile Ala Asp Val Pro Glu Gly Tyr Thr Pro Thr Thr Trp
310 315 320 325
ttg cac cat gaa gtg atg gct ggc ttg gag gat cgt ttc tct gga cag 1123
Leu His His Glu Val Met Ala Gly Leu Glu Asp Arg Phe Ser Gly Gln
330 335 340
caa gtt cct gag gat tat att gag cgc gcg gag tat gag atc tcc gtt 1171
Gln Val Pro Glu Asp Tyr Ile Glu Arg Ala Glu Tyr Glu Ile Ser Val
345 350 355
att gat atg aag ggc tac cct tcc tac ttc ctc atc gtt gct gaa att 1219
Ile Asp Met Lys Gly Tyr Pro Ser Tyr Phe Leu Ile Val Ala Glu Ile
360 365 370
atc aag cac gct cgt tcc att ggc atc cgt gta gga cct ggc cgt ggt 1267
Ile Lys His Ala Arg Ser Ile Gly Ile Arg Val Gly Pro Gly Arg Gly
375 380 385
tcg gct gca ggt gca ttg gtt gcc tac gcg ttg acc atc acc aac att 1315
Ser Ala Ala Gly Ala Leu Val Ala Tyr Ala Leu Thr Ile Thr Asn Ile
390 395 400 405
gac ccc atg gaa cac gga ttg ctg ttc gag aga ttc ctc aac cca gaa 1363
Asp Pro Met Glu His Gly Leu Leu Phe Glu Arg Phe Leu Asn Pro Glu
410 415 420
cga cca tcc gca ccc gat atc gat att gac ttc gat gat cgc cgc cgc 1411
Arg Pro Ser Ala Pro Asp Ile Asp Ile Asp Phe Asp Asp Arg Arg Arg
425 430 435
ggt gaa atg atc cgt tac gca gct gac cgt tgg ggc gag gac aag att 1459
Gly Glu Met Ile Arg Tyr Ala Ala Asp Arg Trp Gly Glu Asp Lys Ile
440 445 450
gct cag gtg atc acc ttc ggt acg gtg aaa aca aag cag gca ctg aaa 1507
Ala Gln Val Ile Thr Phe Gly Thr Val Lys Thr Lys Gln Ala Leu Lys
455 460 465
gac tcc gcc cgt gtg caa atg ggg cag cca ggc tat caa atc gct gac 1555
Asp Ser Ala Arg Val Gln Met Gly Gln Pro Gly Tyr Gln Ile Ala Asp
470 475 480 485
cgc gtg atc aag gag ctt cca cct gcc att atg gcg aag gat att ccg 1603
Arg Val Ile Lys Glu Leu Pro Pro Ala Ile Met Ala Lys Asp Ile Pro
490 495 500
ttg tcc ggt atc acc gat ccg gat cac cct cgt ttt aat gaa gca ggt 1651
Leu Ser Gly Ile Thr Asp Pro Asp His Pro Arg Phe Asn Glu Ala Gly
505 510 515
gcg gtg cgc cag ctg atc gaa acc gac cct gat gtg aag cgc att tac 1699
Ala Val Arg Gln Leu Ile Glu Thr Asp Pro Asp Val Lys Arg Ile Tyr
520 525 530
gac acc gct cgt ggt ttg gaa ggc gtg gtg cgc caa tct ggc gtg cac 1747
Asp Thr Ala Arg Gly Leu Glu Gly Val Val Arg Gln Ser Gly Val His
535 540 545
gcg tgt gcc gtg att atg tct tct gta ccg ctg ctg gac tgc att ccg 1795
Ala Cys Ala Val Ile Met Ser Ser Val Pro Leu Leu Asp Cys Ile Pro
550 555 560 565
atg tgg aag cgg cca gcc gac ggc gca ctg att aca ggc tgg gat tac 1843
Met Trp Lys Arg Pro Ala Asp Gly Ala Leu Ile Thr Gly Trp Asp Tyr
570 575 580
cca gca tgt gag gcc att ggc ctg ttg aag atg gac ttc ctg gga ctt 1891
Pro Ala Cys Glu Ala Ile Gly Leu Leu Lys Met Asp Phe Leu Gly Leu
585 590 595
cga aac ctt acc gtt att ggc gat gcg att gaa aac att aag gcc aac 1939
Arg Asn Leu Thr Val Ile Gly Asp Ala Ile Glu Asn Ile Lys Ala Asn
600 605 610
cgc gat ggg gaa gtg ctt gat cta gaa aac cta gcg atc gag gat gaa 1987
Arg Asp Gly Glu Val Leu Asp Leu Glu Asn Leu Ala Ile Glu Asp Glu
615 620 625
gaa acc tac aag ctg cta ggc cgt gga gaa acc ctt ggt gtg ttc cag 2035
Glu Thr Tyr Lys Leu Leu Gly Arg Gly Glu Thr Leu Gly Val Phe Gln
630 635 640 645
ctt gat ggt ggt ggc atg cag gag ctg ctg aag cgt atg cag cca acc 2083
Leu Asp Gly Gly Gly Met Gln Glu Leu Leu Lys Arg Met Gln Pro Thr
650 655 660
ggc ttc aat gac atc gtc gca gcg ctt gcg ctg tat cgc cca ggc ccc 2131
Gly Phe Asn Asp Ile Val Ala Ala Leu Ala Leu Tyr Arg Pro Gly Pro
665 670 675
atg ggt gtg aac gcg cac tgg gat tac gcc gac cgt aaa aac gga cgc 2179
Met Gly Val Asn Ala His Trp Asp Tyr Ala Asp Arg Lys Asn Gly Arg
680 685 690
aag ccc att act cca att cac cca gag ttg gag gaa gct ctg gaa gaa 2227
Lys Pro Ile Thr Pro Ile His Pro Glu Leu Glu Glu Ala Leu Glu Glu
695 700 705
atc ctt ggt gaa acc tat ggt ctg att gtg tac cag gag cag atc atg 2275
Ile Leu Gly Glu Thr Tyr Gly Leu Ile Val Tyr Gln Glu Gln Ile Met
710 715 720 725
agg atc tcc cag aag gtc gca aac tac acc gct ggt caa gca gat ggt 2323
Arg Ile Ser Gln Lys Val Ala Asn Tyr Thr Ala Gly Gln Ala Asp Gly
730 735 740
ttc cgt aaa gcc atg ggt aag aag aag ccc gag gtc ctg gaa aag gag 2371
Phe Arg Lys Ala Met Gly Lys Lys Lys Pro Glu Val Leu Glu Lys Glu
745 750 755
ttc gca aac ttc gaa ggt gga atg aag gcg aac ggc tat tca gat gcc 2419
Phe Ala Asn Phe Glu Gly Gly Met Lys Ala Asn Gly Tyr Ser Asp Ala
760 765 770
gcg att aag act ttg tgg gat acc att ctg ccg ttc gcc ggc tac gcg 2467
Ala Ile Lys Thr Leu Trp Asp Thr Ile Leu Pro Phe Ala Gly Tyr Ala
775 780 785
ttc aac aag tct cac gcc gca ggt tat gga ctt gta tcc ttc tgg act 2515
Phe Asn Lys Ser His Ala Ala Gly Tyr Gly Leu Val Ser Phe Trp Thr
790 795 800 805
gcc tat ttg aag gcg cac tac gcg ccg gaa tac atg gca gca ctg ctg 2563
Ala Tyr Leu Lys Ala His Tyr Ala Pro Glu Tyr Met Ala Ala Leu Leu
810 815 820
act tct gtg ggt gat aac aag gac aaa tcg gcg atc tac ctt tct gat 2611
Thr Ser Val Gly Asp Asn Lys Asp Lys Ser Ala Ile Tyr Leu Ser Asp
825 830 835
tgc cga cac ctt gga att cga gtg ctt tcg ccg gat att aac gag tcc 2659
Cys Arg His Leu Gly Ile Arg Val Leu Ser Pro Asp Ile Asn Glu Ser
840 845 850
tcg ttg aac ttc ctt cct gtt ggc acc gat att cgc tat ggc ctg gga 2707
Ser Leu Asn Phe Leu Pro Val Gly Thr Asp Ile Arg Tyr Gly Leu Gly
855 860 865
gcc att aga aac gtg ggt gct gaa gtt gtg gat tcc att ttg gat acc 2755
Ala Ile Arg Asn Val Gly Ala Glu Val Val Asp Ser Ile Leu Asp Thr
870 875 880 885
cgc aag gaa aag ggc cta ttt aag gac ttc tca gac tac ttg gac aag 2803
Arg Lys Glu Lys Gly Leu Phe Lys Asp Phe Ser Asp Tyr Leu Asp Lys
890 895 900
atc gat acc ctg ccg tgt aac aag cgc atc acc gag tct ttg atc aag 2851
Ile Asp Thr Leu Pro Cys Asn Lys Arg Ile Thr Glu Ser Leu Ile Lys
905 910 915
ggt ggc gct ttt gac tcc ctt gga cac gca cga aaa ggc ctc atg ctg 2899
Gly Gly Ala Phe Asp Ser Leu Gly His Ala Arg Lys Gly Leu Met Leu
920 925 930
gtc ttc gaa gat gcc gtt gat tcc gtc atc gct acc aaa aaa gct gct 2947
Val Phe Glu Asp Ala Val Asp Ser Val Ile Ala Thr Lys Lys Ala Ala
935 940 945
gac aag gga caa ttt gat ctc ttt gca gct ttc gac tcg gat aac aac 2995
Asp Lys Gly Gln Phe Asp Leu Phe Ala Ala Phe Asp Ser Asp Asn Asn
950 955 960 965
gac gat gtg gca agt ttc ttc cag atc acc gtt cct gat gac gaa tgg 3043
Asp Asp Val Ala Ser Phe Phe Gln Ile Thr Val Pro Asp Asp Glu Trp
970 975 980
gac cgt aag cat gag ctc gca ctc gag cga gaa atg ctg ggt ctg tat 3091
Asp Arg Lys His Glu Leu Ala Leu Glu Arg Glu Met Leu Gly Leu Tyr
985 990 995
gtt tct gga cac cca ctc gat ggc tat gaa gat gcc att gct gcc cag 3139
Val Ser Gly His Pro Leu Asp Gly Tyr Glu Asp Ala Ile Ala Ala Gln
1000 1005 1010
gtt gat aca gca ctg acc acc att gtt gcc ggt gaa ctc aag cac ggc 3187
Val Asp Thr Ala Leu Thr Thr Ile Val Ala Gly Glu Leu Lys His Gly
1015 1020 1025
gca gaa gtg acc gtg ggt ggc att atc tct ggt gtg gat cga cgg ttc 3235
Ala Glu Val Thr Val Gly Gly Ile Ile Ser Gly Val Asp Arg Arg Phe
1030 1035 1040 1045
tcc aag aag gac ggt tcc cct tgg gcg att gtc acc att gaa gat cac 3283
Ser Lys Lys Asp Gly Ser Pro Trp Ala Ile Val Thr Ile Glu Asp His
1050 1055 1060
aac ggc gcg tcc gtt gaa ttg ttg gtc ttc aac aag gtg tat tcc atc 3331
Asn Gly Ala Ser Val Glu Leu Leu Val Phe Asn Lys Val Tyr Ser Ile
1065 1070 1075
gtt gga tcc atg att gtg gaa gac aac atc atc ttg gcc aag gca cac 3379
Val Gly Ser Met Ile Val Glu Asp Asn Ile Ile Leu Ala Lys Ala His
1080 1085 1090
atc tcc att cga gat gat cgt atg agc ctt ttc tgt gat gat ctc cgc 3427
Ile Ser Ile Arg Asp Asp Arg Met Ser Leu Phe Cys Asp Asp Leu Arg
1095 1100 1105
gtt cca gag ctt ggg cca gga aac ggg caa gga ctt ccg ctt cgt ttg 3475
Val Pro Glu Leu Gly Pro Gly Asn Gly Gln Gly Leu Pro Leu Arg Leu
1110 1115 1120 1125
tcc atg cgt act gat cag tgc acc atg tcc aac att gcc aag ctc aag 3523
Ser Met Arg Thr Asp Gln Cys Thr Met Ser Asn Ile Ala Lys Leu Lys
1130 1135 1140
cag gtg ctg gtg gac aac aag ggt gaa tct gat gtg tac ctc aat ttg 3571
Gln Val Leu Val Asp Asn Lys Gly Glu Ser Asp Val Tyr Leu Asn Leu
1145 1150 1155
atc gat ggg gat aac tcc acg gtc atg att ttg ggt gat cac tta aga 3619
Ile Asp Gly Asp Asn Ser Thr Val Met Ile Leu Gly Asp His Leu Arg
1160 1165 1170
gtc aac cga tcc gca agt ttg atg ggc gac ctc aag gca acg atg ggg 3667
Val Asn Arg Ser Ala Ser Leu Met Gly Asp Leu Lys Ala Thr Met Gly
1175 1180 1185
cca ggc atc ctc ggt taatcacatc acactgggat taccccgtgt 3712
Pro Gly Ile Leu Gly
1190
<210>66
<211>1194
<212>PRT
<213>谷氨酸棒状杆菌
<400>66
Met Ala Arg Leu Ser His Met Ala Lys Gln Ser Ser Phe Val His Leu
1 5 10 15
His Asn His Thr Glu Phe Ser Met Leu Asp Gly Met Ala Lys Ile Asp
20 25 30
Met Leu Ala Asp Glu Val Lys Ala Gln Gly Met Pro Ala Val Gly Ile
35 40 45
Thr Asp His Gly Asn Met Tyr Gly Ser Asn Pro Phe Tyr Arg Lys Met
50 55 60
Thr Glu Met Gly Ile Lys Pro Ile Ile Gly Ile Glu Thr Tyr Met Ala
65 70 75 80
Pro Glu Ser Arg Phe Lys Lys Glu Arg Val Arg Trp Gly Glu Pro His
85 90 95
Gln Lys Ser Asp Asp Val Ser Gly Ser Gly Ala Tyr Leu His Gln Thr
100 105 110
Met Leu Ala Glu Asn Thr Thr Gly Leu Arg Asn Leu Phe Tyr Leu Ser
115 120 125
Ser Met Ala Ser Tyr Glu Gly Gln Leu Gly Lys Trp Pro Arg Met Asp
130 135 140
Ala Asp Ile Ile Ala Glu His Ala Glu Gly Ile Ile Ala Thr Thr Gly
145 150 155 160
Cys Pro Ser Gly Asp Val Gln Thr Arg Leu Arg Leu Gly Gln Phe Asp
165 170 175
Glu Ala Leu Glu Ala Ala Ala Met Trp Gln Asp Ile Tyr Gly Arg Asp
180 185 190
Asn Tyr Phe Leu Glu Leu Met Asp His Gly Leu Asp Ile Glu Thr Arg
195 200 205
Val Arg Ser Glu Leu Leu Glu Ile Gly Arg Lys Leu Asn Leu Pro Pro
210 215 220
Leu Val Thr Asn Asp Cys His Tyr Val Leu Glu Ser Gln Ala Gln Ala
225 230 235 240
His Glu Ala Met Leu Cys Val Gln Thr Gly Lys Thr Leu His Asp Glu
245 250 255
Asp Arg Phe Lys Phe Gly Gly Thr Gly Tyr Tyr Val Lys Ser Ala Glu
260 265 270
Gln Met Arg Ala Leu Trp Asp Asp Met Val Pro Asp Gly Cys Asp Asn
275 280 285
Thr Leu Trp Ile Ala Glu Arg Val Gln Ser Tyr Asp Glu Ile Trp Glu
290 295 300
Glu His Ser His Asp Arg Met Pro Ile Ala Asp Val Pro Glu Gly Tyr
305 310 315 320
Thr Pro Thr Thr Trp Leu His His Glu Val Met Ala Gly Leu Glu Asp
325 330 335
Arg Phe Ser Gly Gln Gln Val Pro Glu Asp Tyr Ile Glu Arg Ala Glu
340 345 350
Tyr Glu Ile Ser Val Ile Asp Met Lys Gly Tyr Pro Ser Tyr Phe Leu
355 360 365
Ile Val Ala Glu Ile Ile Lys His Ala Arg Ser Ile Gly Ile Arg Val
370 375 380
Gly Pro Gly Arg Gly Ser Ala Ala Gly Ala Leu Val Ala Tyr Ala Leu
385 390 395 400
Thr Ile Thr Asn Ile Asp Pro Met Glu His Gly Leu Leu Phe Glu Arg
405 410 415
Phe Leu Asn Pro Glu Arg Pro Ser Ala Pro Asp Ile Asp Ile Asp Phe
420 425 430
Asp Asp Arg Arg Arg Gly Glu Met Ile Arg Tyr Ala Ala Asp Arg Trp
435 440 445
Gly Glu Asp Lys Ile Ala Gln Val Ile Thr Phe Gly Thr Val Lys Thr
450 455 460
Lys Gln Ala Leu Lys Asp Ser Ala Arg Val Gln Met Gly Gln Pro Gly
465 470 475 480
Tyr Gln Ile Ala Asp Arg Val Ile Lys Glu Leu Pro Pro Ala Ile Met
485 490 495
Ala Lys Asp Ile Pro Leu Ser Gly Ile Thr Asp Pro Asp His Pro Arg
500 505 510
Phe Asn Glu Ala Gly Ala Val Arg Gln Leu Ile Glu Thr Asp Pro Asp
515 520 525
Val Lys Arg Ile Tyr Asp Thr Ala Arg Gly Leu Glu Gly Val Val Arg
530 535 540
Gln Ser Gly Val His Ala Cys Ala Val Ile Met Ser Ser Val Pro Leu
545 550 555 560
Leu Asp Cys Ile Pro Met Trp Lys Arg Pro Ala Asp Gly Ala Leu Ile
565 570 575
Thr Gly Trp Asp Tyr Pro Ala Cys Glu Ala Ile Gly Leu Leu Lys Met
580 585 590
Asp Phe Leu Gly Leu Arg Asn Leu Thr Val Ile Gly Asp Ala Ile Glu
595 600 605
Asn Ile Lys Ala Asn Arg Asp Gly Glu Val Leu Asp Leu Glu Asn Leu
610 615 620
Ala Ile Glu Asp Glu Glu Thr Tyr Lys Leu Leu Gly Arg Gly Glu Thr
625 630 635 640
Leu Gly Val Phe Gln Leu Asp Gly Gly Gly Met Gln Glu Leu Leu Lys
645 650 655
Arg Met Gln Pro Thr Gly Phe Asn Asp Ile Val Ala Ala Leu Ala Leu
660 665 670
Tyr Arg Pro Gly Pro Met Gly Val Asn Ala His Trp Asp Tyr Ala Asp
675 680 685
Arg Lys Asn Gly Arg Lys Pro Ile Thr Pro Ile His Pro Glu Leu Glu
690 695 700
Glu Ala Leu Glu Glu Ile Leu Gly Glu Thr Tyr Gly Leu Ile Val Tyr
705 710 715 720
Gln Glu Gln Ile Met Arg Ile Ser Gln Lys Val Ala Asn Tyr Thr Ala
725 730 735
Gly Gln Ala Asp Gly Phe Arg Lys Ala Met Gly Lys Lys Lys Pro Glu
740 745 750
Val Leu Glu Lys Glu Phe Ala Asn Phe Glu Gly Gly Met Lys Ala Asn
755 760 765
Gly Tyr Ser Asp Ala Ala Ile Lys Thr Leu Trp Asp Thr Ile Lau Pro
770 775 780
Phe Ala Gly Tyr Ala Phe Asn Lys Ser His Ala Ala Gly Tyr Gly Leu
785 790 795 800
Val Ser Phe Trp Thr Ala Tyr Leu Lys Ala His Tyr Ala Pro Glu Tyr
805 810 815
Met Ala Ala Leu Leu Thr Ser Val Gly Asp Asn Lys Asp Lys Ser Ala
820 825 830
Ile Tyr Leu Ser Asp Cys Arg His Leu Gly Ile Arg Val Leu Ser Pro
835 840 845
Asp Ile Asn Glu Ser Ser Leu Asn Phe Leu Pro Val Gly Thr Asp Ile
850 855 860
Arg Tyr Gly Leu Gly Ala Ile Arg Asn Val Gly Ala Glu Val Val Asp
865 870 875 880
Ser Ile Leu Asp Thr Arg Lys Glu Lys Gly Leu Phe Lys Asp Phe Ser
885 890 895
Asp Tyr Leu Asp Lys Ile Asp Thr Leu Pro Cys Asn Lys Arg Ile Thr
900 905 910
Glu Ser Leu Ile Lys Gly Gly Ala Phe Asp Ser Leu Gly His Ala Arg
915 920 925
Lys Gly Leu Met Leu Val Phe Glu Asp Ala Val Asp Ser Val Ile Ala
930 935 940
Thr Lys Lys Ala Ala Asp Lys Gly Gln Phe Asp Leu Phe Ala Ala Phe
945 950 955 960
Asp Ser Asp Asn Asn Asp Asp Val Ala Ser Phe Phe Gln Ile Thr Val
965 970 975
Pro Asp Asp Glu Trp Asp Arg Lys His Glu Leu Ala Leu Glu Arg Glu
980 985 990
Met Leu Gly Leu Tyr Val Ser Gly His Pro Leu Asp Gly Tyr Glu Asp
995 1000 1005
Ala Ile Ala Ala Gln Val Asp Thr Ala Leu Thr Thr Ile Val Ala Gly
1010 1015 1020
Glu Leu Lys His Gly Ala Glu Val Thr Val Gly Gly Ile Ile Ser Gly
1025 1030 1035 1040
Val Asp Arg Arg Phe Ser Lys Lys Asp Gly Ser Pro Trp Ala Ile Val
1045 1050 1055
Thr Ile Glu Asp His Asn Gly Ala Ser Val Glu Leu Leu Val Phe Asn
1060 1065 1070
Lys Val Tyr Ser Ile Val Gly Ser Met Ile Val Glu Asp Asn Ile Ile
1075 1080 1085
Leu Ala Lys Ala His Ile Ser Ile Arg Asp Asp Arg Met Ser Leu Phe
1090 1095 1100
Cys Asp Asp Leu Arg Val Pro Glu Leu Gly Pro Gly Asn Gly Gln Gly
1105 1110 1115 1120
Leu Pro Leu Arg Leu Ser Met Arg Thr Asp Gln Cys Thr Met Ser Asn
1125 1130 1135
Ile Ala Lys Leu Lys Gln Val Leu Val Asp Asn Lys Gly Glu Ser Asp
1140 1145 1150
Val Tyr Leu Asn Leu Ile Asp Gly Asp Asn Ser Thr Val Met Ile Leu
1155 1160 1165
Gly Asp His Leu Arg Val Asn Arg Ser Ala Ser Leu Met Gly Asp Leu
1170 1175 1180
Lys Ala Thr Met Gly Pro Gly Ile Leu Gly
1185 1190
<210>67
<211>4036
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(4006)
<223>RXA03166
<400>67
ccctccctat agtgattagt tcacctccag actacctggc accgcgtggg ctgctttcac 60
ttggactttc ggcaaagaca agtagaatta gcccctagtc atg act act tct gaa 115
Met Thr Thr Ser Glu
1 5
act gcc cca tca aag gct tcc ctg tat gaa ctt tta gag ggc gta tcc 163
Thr Ala Pro Ser Lys Ala Ser Leu Tyr Glu Leu Leu Glu Gly Val Ser
10 15 20
ctc tcc gac gag cgc acg tttagg cgc cga ctg tcc aaa gcc cgc gcg 211
Leu Ser Asp Glu Arg Thr Phe Arg Arg Arg Leu Ser Lys Ala Arg Ala
25 30 35
ccc aag gcg ctt ggt gcg att aag gct gat atc gat aag gca cgc ttg 259
Pro Lys Ala Leu Gly Ala Ile Lys Ala Asp Ile Asp Lys Ala Arg Leu
40 45 50
ctt atc gac gaa aag agc cag tta att ccg tct atc acc tac cca gaa 307
Leu Ile Asp Glu Lys Ser Gln Leu Ile Pro Ser Ile Thr Tyr Pro Glu
55 60 65
aac ctt ccg gtg agt tcc cgg cgc gat gat atc gcc gag gct atc cgt 355
Asn Leu Pro Val Ser Ser Arg Arg Asp Asp Ile Ala Glu Ala Ile Arg
70 75 80 85
gat aat cag gtg gtt att atc gcc ggt gag act ggt tcg ggt aag acg 403
Asp Asn Gln Val Val Ile Ile Ala Gly Glu Thr Gly Ser Gly Lys Thr
90 95 100
act cag att cct aag att tgt ttg gac cta ggc cgt ggc cgg cgt ggg 451
Thr Gln Ile Pro Lys Ile Cys Leu Asp Leu Gly Arg Gly Arg Arg Gly
105 110 115
ctc att ggc cac aca cag cca cgt cga tta gca gct agg acc gtc gcc 499
Leu Ile Gly His Thr Gln Pro Arg Arg Leu Ala Ala Arg Thr Val Ala
120 125 130
gag cgc atc gcc gat gaa ttg ggg caa gac atc ggc gaa tcg gtg ggt 547
Glu Arg Ile Ala Asp Glu Leu Gly Gln Asp Ile Gly Glu Ser Val Gly
135 140 145
tat gcg att cgt ttt gat gat cga gtt tcc tcg cat aca tcc gtg aag 595
Tyr Ala Ile Arg Phe Asp Asp Arg Val Ser Ser His Thr Ser Val Lys
150 155 160 165
ttg atg scg gat ggt att ttg ctt gct gaa atg cag cgg gat cgt ttc 643
Leu Met Thr Asp Gly Ile Leu Leu Ala Glu Met Gln Arg Asp Arg Phe
170 175 180
ctc aat gcg tat gac acc atc atc atc gat gag gcg cat gaa cgt tcc 691
Leu Asn Ala Tyr Asp Thr Ile Ile Ile Asp Glu Ala His Glu Arg Ser
185 190 195
ctc aac att gac ttc att ctg ggt tat ttg cgc cag ttg ttg cct aag 739
Leu Asn Ile Asp Phe Ile Leu Gly Tyr Leu Arg Gln Leu Leu Pro Lys
200 205 210
cgc cct gat ctt aaa gtc att att acc tcc gca acg att gac cct gag 787
Arg Pro Asp Leu Lys Val Ile Ile Thr Ser Ala Thr Ile Asp Pro Glu
215 220 225
cgt ttc gcg gag cac ttt gct gat gct tct gga aaa cca gca cca att 835
Arg Phe Ala Glu His Phe Ala Asp Ala Ser Gly Lys Pro Ala Pro Ile
230 235 240 245
atc gag gtc tcc ggc cgc acc ttc ccg gta gag atc cgt tat cgc cca 883
Ile Glu Val Ser Gly Arg Thr Phe Pro Val Glu Ile Arg Tyr Arg Pro
250 255 260
ctt gag gta tta gac ggc gat aaa att atc gat acc gat ccc ctt gac 931
Leu Glu Val Leu Asp Gly Asp Lys Ile Ile Asp Thr Asp Pro Leu Asp
265 270 275
ggc ttg tgt tct gct ttg gaa gag ctc atg gct gaa ggc gac ggc gat 979
Gly Leu Cys Ser Ala Leu Glu Glu Leu Met Ala Glu Gly Asp Gly Asp
280 285 290
atc ctc tgc ttc ttt gcc ggt gag cgt gat atc cgc gat gcc atg gag 1027
Ile Leu Cys Phe Phe Ala Gly Glu Arg Asp Ile Arg Asp Ala Met Glu
295 300 305
gca atc gag gcc cga cgc tgg aaa ggt gtg gaa gtc act ccc ctg ttt 1075
Ala Ile Glu Ala Arg Arg Trp Lys Gly Val Glu Val Thr Pro Leu Phe
310 315 320 325
ggc cgc ctg tcc aac cag gag cag cac cgt gta ttt agc ccg cac tct 1123
Gly Arg Leu Ser Asn Gln Glu Gln His Arg Val Phe Ser Pro His Ser
330 335 340
ggt cga cgc att gtg ctt tcc acc aat atc gca gag aca tcc ctg acg 1171
Gly Arg Arg Ile Val Leu Ser Thr Asn Ile Ala Glu Thr Ser Leu Thr
345 350 355
gtt ccg ggc att cac tac gtg gtt gat acc ggt acg gcg cgt atc tct 1219
Val Pro Gly Ile His Tyr Val Val Asp Thr Gly Thr Ala Arg Ile Ser
360 365 370
cgt tat tca gtg cgc acc aag gtg cag cgc ctt ccg att gag aac att 1267
Arg Tyr Ser Val Arg Thr Lys Val Gln Arg Leu Pro Ile Glu Asn Ile
375 380 385
tcc cag gcc agc gca aac cag cgt tct ggt cgt tgt ggt cgt gtc gca 1315
Ser Gln Ala Ser Ala Asn Gln Arg Ser Gly Arg Cys Gly Arg Val Ala
390 395 400 405
gac ggt att gcg att cgt ttg tat tcc gaa gat gat ttc aac tct cgc 1363
Asp Gly Ile Ala Ile Arg Leu Tyr Ser Glu Asp Asp Phe Asn Ser Arg
410 415 420
cca gag ttc acc gat ccg gaa att ctg cgc acc aac ttg gcg agc gtt 1411
Pro Glu Phe Thr Asp Pro Glu Ile Leu Arg Thr Asn Leu Ala Ser Val
425 430 435
att ttg cgc atg gcg tcg ctg cgt ctt ggc gat att aat gat ttc ccc 1459
Ile Leu Arg Met Ala Ser Leu Arg Leu Gly Asp Ile Asn Asp Phe Pro
440 445 450
ttc gtc caa gcc cca gag caa cgc tcc atc agg gat ggt att ttg ctg 1507
Phe Val Gln Ala Pro Glu Gln Arg Ser Ile Arg Asp Gly Ile Leu Leu
455 460 465
ctc cac gag ttg ggc gca ctc acc gac gac acc caa gcc gat ggt tca 1555
Leu His Glu Leu Gly Ala Leu Thr Asp Asp Thr Gln Ala Asp Gly Ser
470 475 480 485
ccg cag ctc acc cag atc ggt aaa gac tta gcc aac atc cca gtc gat 1603
Pro Gln Leu Thr Gln Ile Gly Lys Asp Leu Ala Asn Ile Pro Val Asp
490 495 500
ccc cgc atg gcc cgc atg ctc gta gaa gcc aac act cta ggc tgc ctg 1651
Pro Arg Met Ala Arg Met Leu Val Glu Ala Asn Thr Leu Gly Cys Leu
505 510 515
cat tct gtc atg gtc att gtg tcc gct ctg acg att caa gat gtt cgc 1699
His Ser Val Met Val Ile Val Ser Ala Leu Thr Ile Gln Asp Val Arg
520 525 530
gaa cgc ccc ctg gaa ttc caa gcc caa gct gat caa gcc cac gct agg 1747
Glu Arg Pro Leu Glu Phe Gln Ala Gln Ala Asp Gln Ala His Ala Arg
535 540 545
ttc aag gac acc acc tca gat ttc tta ggc ttt ttg aaa ctg tgg gag 1795
Phe Lys Asp Thr Thr Ser Asp Phe Leu Gly Phe Leu Lys Leu Trp Glu
550 555 560 565
tac atc gcc gat cag cgc aat caa agc agc ggc aat tcc ttc cgc aag 1843
Tyr Ile Ala Asp Gln Arg Asn Gln Ser Ser Gly Asn Ser Phe Arg Lys
570 575 580
cag atg aaa aaa gaa ttc ctc cac tac atg cgt atc cgc gag tgg tgg 1891
Gln Met Lys Lys Glu Phe Leu His Tyr Met Arg Ile Arg Glu Trp Trp
585 590 595
gat ttg gtg cgc caa tta gag cag att ggc cag caa ctt ggt tgg gca 1939
Asp Leu Val Arg Gln Leu Glu Gln Ile Gly Gln Gln Leu Gly Trp Ala
600 605 610
aag aag gag cag gtt gca ggc acc gcc agc cct gac atc att cac caa 1987
Lys Lys Glu Gln Val Ala Gly Thr Ala Ser Pro Asp Ile Ile His Gln
615 620 625
tcc ttg ctc acc ggt ctg ctc tcg caa atc ggt tcc cgt gat ggt gag 2035
Ser Leu Leu Thr Gly Leu Leu Ser Gln Ile Gly Ser Arg Asp Gly Glu
630 635 640 645
agc aaa gaa ttc act gga gcc aga ggt acc aaa ttc ttg gtc ttt cct 2083
Ser Lys Glu Phe Thr Gly Ala Arg Gly Thr Lys Phe Leu Val Phe Pro
650 655 660
ggt tct gcg cta acc aag aag ccg cca cag ttc atc atg gct ggc caa 2131
Gly Ser Ala Leu Thr Lys Lys Pro Pro Gln Phe Ile Met Ala Gly Gln
665 670 675
ttg gta gaa acc tca cgt ttg tgg gct cgc gat gta gcc aag att gaa 2179
Leu Val Glu Thr Ser Arg Leu Trp Ala Arg Asp Val Ala Lys Ile Glu
680 685 690
cca gag tgg gtg gaa aaa gca gcc ggc cca ttg ctc aag cac caa tac 2227
Pro Glu Trp Val Glu Lys Ala Ala Gly Pro Leu Leu Lys His Gln Tyr
695 700 705
tct gaa ccg tat tgg tcc tca aag cgt ggc gct gcc atg gtg cac egc 2275
Ser Glu Pro Tyr Trp Ser Ser Lys Arg Gly Ala Ala Met Val His Arg
710 715 720 725
aaa tca acg ctc tac ggt gtg aca att gtt gcc gac aag gta gtt ccc 2323
Lys Ser Thr Leu Tyr Gly Val Thr Ile Val Ala Asp Lys Val Val Pro
730 735 740
tac cac acg gtt gat ccc gtg gct gcg cgc gat atg ttc atc cgc cat 2371
Tyr His Thr Val Asp Pro Val Ala Ala Arg Asp Met Phe Ile Arg His
745 750 755
gcc ctc atc gaa ggc gat tgg tcc act cac cac cgc ttc tac cac gac 2419
Ala Leu Ile Glu Gly Asp Trp Ser Thr His His Arg Phe Tyr His Asp
760 765 770
aac gtg gcc aaa ttg gag gca atc gga gaa ctt gaa gcc aag gcg cgt 2467
Asn Val Ala Lys Leu Glu Ala Ile Gly Glu Leu Glu Ala Lys Ala Arg
775 780 785
cgc cgc gac atc gtc gta gat gaa gac acc ctg ttt gat ttc tac gat 2515
Arg Arg Asp Ile Val Val Asp Glu Asp Thr Leu Phe Asp Phe Tyr Asp
790 795 800 805
gcc aag ctt cca agc aac gcc acc acc acc cgc aat ttc gat tcc tgg 2563
Ala Lys Leu Pro Ser Asn Ala Thr Thr Thr Arg Asn Phe Asp Ser Trp
810 815 820
tgg aag aaa aca tca cgc gtc aca cca gat ctt ctc gat ttc gac cca 2611
Trp Lys Lys Thr Ser Arg Val Thr Pro Asp Leu Leu Asp Phe Asp Pro
825 830 835
gat agt ctg atc aac gaa gac gct gac ggc gtt aac gaa gaa gcc ttc 2659
Asp Ser Leu Ile Asn Glu Asp Ala Asp Gly Val Asn Glu Glu Ala Phe
840 845 850
ccc gac aag tgg att aaa ggc agc ctg atc ttc gat ctc agc tac cac 2707
Pro Asp Lys Trp Ile Lys Gly Ser Leu Ile Phe Asp Leu Ser Tyr His
855 860 865
ttc gaa ccc ggc cac cgc gat gac ggc gtc aca gtc ctc atc ccc att 2755
Phe Glu Pro Gly His Arg Asp Asp Gly Val Thr Val Leu Ile Pro Ile
870 875 880 885
cca gtg ctg ggt gga ctt gat acc gaa ggc ttc gat tgg cta gtt cca 2803
Pro Val Leu Gly Gly Leu Asp Thr Glu Gly Phe Asp Trp Leu Val Pro
890 895 900
ggc tta agg ctt gat ttg gtg acc gag tta atc cgc acc atg cct aag 2851
Gly Leu Arg Leu Asp Leu Val Thr Glu Leu Ile Arg Thr Met Pro Lys
905 910 915
gct tta cga cgc acc gtt gtc cct gcc ccg gat ttc gcc gag cgg gtc 2899
Ala Leu Arg Arg Thr Val Val Pro Ala Pro Asp Phe Ala Glu Arg Val
920 925 930
ttg ccg tta ctg cgt ccc tac atg aca cca ctg acc acc caa ctt gcc 2947
Leu Pro Leu Leu Arg Pro Tyr Met Thr Pro Leu Thr Thr Gln Leu Ala
935 940 945
gat gca ctc cat acc ctg ggc ggc caa gga atc aac gcc tct gat ttc 2995
Asp Ala Leu His Thr Leu Gly Gly Gln Gly Ile Asn Ala Ser Asp Phe
950 955 960 965
gat cct gcc aaa ctc ccc gat cac ctg cgc att tcc tat gca gca gta 3043
Asp Pro Ala Lys Leu Pro Asp His Leu Arg Ile Ser Tyr Ala Ala Val
970 975 980
gat cgt cgt ggc aag atc atc gac cgc gac cgt gac ctc gaa gca cta 3091
Asp Arg Arg Gly Lys Ile Ile Asp Arg Asp Arg Asp Leu Glu Ala Leu
985 990 995
aag aaa cgc cgc gcg aac caa atc agg gca tct gtt tct agc gtt gct 3139
Lys Lys Arg Arg Ala Asn Gln Ile Arg Ala Ser Val Ser Ser Val Ala
1000 1005 1010
aag gca tcc gaa caa gat gaa act acg cag tgg acc aca gat acc ttg 3187
Lys Ala Ser Glu Gln Asp Glu Thr Thr Gln Trp Thr Thr Asp Thr Leu
1015 1020 1025
ggc acc atc gat gaa gaa atc gag acc act gtc gat gga cag tcc gtc 3235
Gly Thr Ile Asp Glu Glu Ile Glu Thr Thr Val Asp Gly Gln Ser Val
1030 1035 1040 1045
acc gcg tac ccc gcc ttg gtt gtc acc ccg act ggc gta tcc gtc aag 3283
Thr Ala Tyr Pro Ala Leu Val Val Thr Pro Thr Gly Val Ser Val Lys
1050 1055 1060
gtc atg ccc acc aag gct gct gct gat gcc tcg atg atc acc gca aca 3331
Val Met Pro Thr Lys Ala Ala Ala Asp Ala Ser Met Ile Thr Ala Thr
1065 1070 1075
ttg aca ctg ttg atg cgt gag att gaa gta tcc aca aag caa atg gtc 3379
Leu Thr Leu Leu Met Arg Glu Ile Glu Val Ser Thr Lys Gln Met Val
1080 1085 1090
aag gga cta ccc ttg caa caa cga gtc gca gtg gac aac tat ccc cac 3427
Lys Gly Leu Pro Leu Gln Gln Arg Val Ala Val Asp Asn Tyr Pro His
1095 1100 1105
ggc ggt gcc gaa gga ctg gtt tct gat gcc cgt atc gcc gca att cgc 3475
Gly Gly Ala Glu Gly Leu Val Ser Asp Ala Arg Ile Ala Ala Ile Arg
1110 1115 1120 1125
gat ttg atg ttt gaa cac ggc gga gct gtg cgt tca ccg gaa gag ttc 3523
Asp Leu Met Phe Glu His Gly Gly Ala Val Arg Ser Pro Glu Glu Phe
1130 1135 1140
gac gtt ctt cta gcc aag gtg aag ccg gaa aca cca gga gtt gtt cgt 3571
Asp Val Leu Leu Ala Lys Val Lys Pro Glu Thr Pro Gly Val Val Arg
1145 1150 1155
cga act gtc gtt gag cta gcc cca gca ttg gtt cac tat gca tcc gtt 3619
Arg Thr Val Val Glu Leu Ala Pro Ala Leu Val His Tyr Ala Ser Val
1160 1165 1170
gtg tca gag ctg gag tcc tgg tct gga cct gcc att gac gat atg acc 3667
Val Ser Glu Leu Glu Ser Trp Ser Gly Pro Ala Ile Asp Asp Met Thr
1175 1180 1185
gcc cag ttg gat ttc ctc ttg cca aag cag gcc atc agc att cac ggc 3715
Ala Gln Leu Asp Phe Leu Leu Pro Lys Gln Ala Ile Ser Ile His Gly
1190 1195 1200 1205
atc agt agg ttg agg cac ctt ccg agg tat ctg cag gcg ttg acg att 3763
Ile Ser Arg Leu Arg His Leu Pro Arg Tyr Leu Gln Ala Leu Thr Ile
1210 1215 1220
cgc cta gaa gag atg aac caa aat ccg gat cgt gac gcc gac cgt caa 3811
Arg Leu Glu Glu Met Asn Gln Asn Pro Asp Arg Asp Ala Asp Arg Gln
1225 1230 1235
gat gaa gtg aac ttc gtt gaa gag cag ctg gag aaa cag ctc agt aag 3859
Asp Glu Val Asn Phe Val Glu Glu Gln Leu Glu Lys Gln Leu Ser Lys
1240 1245 1250
cta ccc gct gcg cga gcc aac acc aag gaa gcc aag gat att gcc tgg 3907
Leu Pro Ala Ala Arg Ala Asn Thr Lys Glu Ala Lys Asp Ile Ala Trp
1255 1260 1265
cag att cag gaa cta agg atc agc ctc ttt gca cag cgg tta ggc act 3955
Gln Ile Gln Glu Leu Arg Ile Ser Leu Phe Ala Gln Arg Leu Gly Thr
1270 1275 1280 1285
ccc cga cct gta tcg gcg cag aga atc cag aag gct att gcc aag ctt 4003
Pro Arg Pro Val Ser Ala Gln Arg Ile Gln Lys Ala Ile Ala Lys Leu
1290 1295 1300
cga taagcactgc ttgagatgag ggactagtta 4036
Arg
<210>68
<211>1302
<212>PRT
<213>谷氨酸棒状杆菌
<400>68
Met Thr Thr Ser Glu Thr Ala Pro Ser Lys Ala Ser Leu Tyr Glu Leu
1 5 10 15
Leu Glu Gly Val Ser Leu Ser Asp Glu Arg Thr Phe Arg Arg Arg Leu
20 25 30
Ser Lys Ala Arg Ala Pro Lys Ala Leu Gly Ala Ile Lys Ala Asp Ile
35 40 45
Asp Lys Ala Arg Leu Leu Ile Asp Glu Lys Ser Gln Leu Ile Pro Ser
50 55 60
Ile Thr Tyr Pro Glu Asn Leu Pro Val Ser Ser Arg Arg Asp Asp Ile
65 70 75 80
Ala Glu Ala Ile Arg Asp Asn Gln Val Val Ile Ile Ala Gly Glu Thr
85 90 95
Gly Ser Gly Lys Thr Thr Gln Ile Pro Lys Ile Cys Leu Asp Leu Gly
100 105 110
Arg Gly Arg Arg Gly Leu Ile Gly His Thr Gln Pro Arg Arg Leu Ala
115 120 125
Ala Arg Thr Val Ala Glu Arg Ile Ala Asp Glu Leu Gly Gln Asp Ile
130 135 140
Gly Glu Ser Val Gly Tyr Ala Ile Arg Phe Asp Asp Arg Val Ser Ser
145 150 155 160
His Thr Ser Val Lys Leu Met Thr Asp Gly Ile Leu Leu Ala Glu Met
165 170 175
Gln Arg Asp Arg Phe Leu Asn Ala Tyr Asp Thr Ile Ile Ile Asp Glu
180 185 190
Ala His Glu Arg Ser Leu Asn Ile Asp Phe Ile Leu Gly Tyr Leu Arg
195 200 205
Gln Leu Leu Pro Lys Arg Pro Asp Leu Lys Val Ile Ile Thr Ser Ala
210 215 220
Thr Ile Asp Pro Glu Arg Phe Ala Glu His Phe Ala Asp Ala Ser Gly
225 230 235 240
Lys Pro Ala Pro Ile Ile Glu Val Ser Gly Arg Thr Phe Pro Val Glu
245 250 255
Ile Arg Tyr Arg Pro Leu Glu Val Leu Asp Gly Asp Lys Ile Ile Asp
260 265 270
Thr Asp Pro Leu Asp Gly Leu Cys Ser Ala Leu Glu Glu Leu Met Ala
275 280 285
Glu Gly Asp Gly Asp Ile Leu Cys Phe Phe Ala Gly Glu Arg Asp Ile
290 295 300
Arg Asp Ala Met Glu Ala Ile Glu Ala Arg Arg Trp Lys Gly Val Glu
305 310 315 320
Val Thr Pro Leu Phe Gly Arg Leu Ser Asn Gln Glu Gln His Arg Val
325 330 335
Phe Ser Pro His Ser Gly Arg Arg Ile Val Leu Ser Thr Asn Ile Ala
340 345 350
Glu Thr Ser Leu Thr Val Pro Gly Ile His Tyr Val Val Asp Thr Gly
355 360 365
Thr Ala Arg Ile Ser Arg Tyr Ser Val Arg Thr Lys Val Gln Arg Leu
370 375 380
Pro Ile Glu Asn Ile Ser Gln Ala Ser Ala Asn Gln Arg Ser Gly Arg
385 390 395 400
Cys Gly Arg Val Ala Asp Gly Ile Ala Ile Arg Leu Tyr Ser Glu Asp
405 410 415
Asp Phe Asn Ser Arg Pro Glu Phe Thr Asp Pro Glu Ile Leu Arg Thr
420 425 430
Asn Leu Ala Ser Val Ile Leu Arg Met Ala Ser Leu Arg Leu Gly Asp
435 440 445
Ile Asn Asp Phe Pro Phe Val Gln Ala Pro Glu Gln Arg Ser Ile Arg
450 455 460
Asp Gly Ile Leu Leu Leu His Glu Leu Gly Ala Leu Thr Asp Asp Thr
465 470 475 480
Gln Ala Asp Gly Ser Pro Gln Leu Thr Gln Ile Gly Lys Asp Leu Ala
485 490 495
Asn Ile Pro Val Asp Pro Arg Met Ala Arg Met Leu Val Glu Ala Asn
500 505 510
Thr Leu Gly Cys Leu His Ser Val Met Val Ile Val Ser Ala Leu Thr
515 520 525
Ile Gln Asp Val Arg Glu Arg Pro Leu Glu Phe Gln Ala Gln Ala Asp
530 535 540
Gln Ala His Ala Arg Phe Lys Asp Thr Thr Ser Asp Phe Leu Gly Phe
545 550 555 560
Leu Lys Leu Trp Glu Tyr Ile Ala Asp Gln Arg Asn Gln Ser Ser Gly
565 570 575
Asn Ser Phe Arg Lys Gln Met Lys Lys Glu Phe Leu His Tyr Met Arg
580 585 590
Ile Arg Glu Trp Trp Asp Leu Val Arg Gln Leu Glu Gln Ile Gly Gln
595 600 605
Gln Leu Gly Trp Ala Lys Lys Glu Gln Val Ala Gly Thr Ala Ser Pro
610 615 620
Asp Ile Ile His Gln Ser Leu Leu Thr Gly Leu Leu Ser Gln Ile Gly
625 630 635 640
Ser Arg Asp Gly Glu Ser Lys Glu Phe Thr Gly Ala Arg Gly Thr Lys
645 650 655
Phe Leu Val Phe Pro Gly Ser Ala Leu Thr Lys Lys Pro Pro Gln Phe
660 665 670
Ile Met Ala Gly Gln Leu Val Glu Thr Ser Arg Leu Trp Ala Arg Asp
675 680 685
Val Ala Lys Ile Glu Pro Glu Trp Val Glu Lys Ala Ala Gly Pro Leu
690 695 700
Leu Lys His Gln Tyr Ser Glu Pro Tyr Trp Ser Ser Lys Arg Gly Ala
705 710 715 720
Ala Met Val His Arg Lys Ser Thr Leu Tyr Gly Val Thr Ile Val Ala
725 730 735
Asp Lys Val Val Pro Tyr His Thr Val Asp Pro Val Ala Ala Arg Asp
740 745 750
Met Phe Ile Arg His Ala Leu Ile Glu Gly Asp Trp Ser Thr His His
755 760 765
Arg Phe Tyr His Asp Asn Val Ala Lys Leu Glu Ala Ile Gly Glu Leu
770 775 780
Glu Ala Lys Ala Arg Arg Arg Asp Ile Val Val Asp Glu Asp Thr Leu
785 790 795 800
Phe Asp Phe Tyr Asp Ala Lys Leu Pro Ser Asn Ala Thr Thr Thr Arg
805 810 815
Asn Phe Asp Ser Trp Trp Lys Lys Thr Ser Arg Val Thr Pro Asp Leu
820 825 830
Leu Asp Phe Asp Pro Asp Ser Leu Ile Asn Glu Asp Ala Asp Gly Val
835 840 845
Asn Glu Glu Ala Phe Pro Asp Lys Trp Ile Lys Gly Ser Leu Ile Phe
850 855 860
Asp Leu Ser Tyr His Phe Glu Pro Gly His Arg Asp Asp Gly Val Thr
865 870 875 880
Val Leu Ile Pro Ile Pro Val Leu Gly Gly Leu Asp Thr Glu Gly Phe
885 890 895
Asp Trp Leu Val Pro Gly Leu Arg Leu Asp Leu Val Thr Glu Leu Ile
900 905 910
Arg Thr Met Pro Lys Ala Leu Arg Arg Thr Val Val Pro Ala Pro Asp
915 920 925
Phe Ala Glu Arg Val Leu Pro Leu Leu Arg Pro Tyr Met Thr Pro Leu
930 935 940
Thr Thr Gln Leu Ala Asp Ala Leu His Thr Leu Gly Gly Gln Gly Ile
945 950 955 960
Asn Ala Ser Asp Phe Asp Pro Ala Lys Leu Pro Asp His Leu Arg Ile
965 970 975
Ser Tyr Ala Ala Val Asp Arg Arg Gly Lys Ile Ile Asp Arg Asp Arg
980 985 990
Asp Leu Glu Ala Leu Lys Lys Arg Arg Ala Asn Gln Ile Arg Ala Ser
995 1000 1005
Val Ser Ser Val Ala Lys Ala Ser Glu Gln Asp Glu Thr Thr Gln Trp
1010 1015 1020
Thr Thr Asp Thr Leu Gly Thr Ile Asp Glu Glu Ile Glu Thr Thr Val
1025 1030 1035 1040
Asp Gly Gln Ser Val Thr Ala Tyr Pro Ala Leu Val Val Thr Pro Thr
1045 1050 1055
Gly Val Ser Val Lys Val Met Pro Thr Lys Ala Ala Ala Asp Ala Ser
1060 1065 1070
Met Ile Thr Ala Thr Leu Thr Leu Leu Met Arg Glu Ile Glu Val Ser
1075 1080 1085
Thr Lys Gln Met Val Lys Gly Leu Pro Leu Gln Gln Arg Val Ala Val
1090 1095 1100
Asp Asn Tyr Pro His Gly Gly Ala Glu Gly Leu Val Ser Asp Ala Arg
1105 1110 1115 1120
Ile Ala Ala Ile Arg Asp Leu Met Phe Glu His Gly Gly Ala Val Arg
1125 1130 1135
Ser Pro Glu Glu Phe Asp Val Leu Leu Ala Lys Val Lys Pro Glu Thr
1140 1145 1150
Pro Gly Val Val Arg Arg Thr Val Val Glu Leu Ala Pro Ala Leu Val
1155 1160 1165
His Tyr Ala Ser Val Val Ser Glu Leu Glu Ser Trp Ser Gly Pro Ala
1170 1175 1180
Ile Asp Asp Met Thr Ala Gln Leu Asp Phe Leu Leu Pro Lys Gln Ala
1185 1190 1195 1200
Ile Ser Ile His Gly Ile Ser Arg Leu Arg His Leu Pro Arg Tyr Leu
1205 1210 1215
Gln Ala Leu Thr Ile Arg Leu Glu Glu Met Asn Gln Asn Pro Asp Arg
1220 1225 1230
Asp Ala Asp Arg Gln Asp Glu Val Asn Phe Val Glu Glu Gln Leu Glu
1235 1240 1245
Lys Gln Leu Ser Lys Leu Pro Ala Ala Arg Ala Asn Thr Lys Glu Ala
1250 1255 1260
Lys Asp Ile Ala Trp Gln Ile Gln Glu Leu Arg Ile Ser Leu Phe Ala
1265 1270 1275 1280
Gln Arg Leu Gly Thr Pro Arg Pro Val Ser Ala Gln Arg Ile Gln Lys
1285 1290 1295
Ala Ile Ala Lys Leu Arg
1300
<210>69
<211>1312
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1282)
<223>RXA03266
<400>69
gaaccttctt ccaagaagtt ttccctatcg gtaaacgtcc gggtttagtt aaacgatgaa 60
ctatatttga tctatatttc ccaaggagct ttaaaacagc atg gag tca caa aac 115
Met Glu Ser Gln Asn
1 5
gtg tcc ttc cgt gtg gcc agg gaa gac ctg gtt acc gcg gta gcc tgg 163
Val Ser Phe Arg Val Ala Arg Glu Asp Leu Val Thr Ala Val Ala Trp
10 15 20
gtc gct cgc aac ctg ccc acc aaa ccg act cag ccg gta ctt cga gcc 211
Val Ala Arg Asn Leu Pro Thr Lys Pro Thr Gln Pro Val Leu Arg Ala
25 30 35
atg ctg atc act gcc gat gat gag ggt ctt gaa ctc gcc ggt tac gac 259
Met Leu Ile Thr Ala Asp Asp Glu Gly Leu Glu Leu Ala Gly Tyr Asp
40 45 50
tac gac gta tcc acc cga gtt cgc ctc tca gct gag gtt tct cag cca 307
Tyr Asp Val Ser Thr Arg Val Arg Leu Ser Ala Glu Val Ser Gln Pro
55 60 65
ggt cgc atc gca gta gcc ggc aag ctg ctg tct gaa atc aca gga tct 355
Gly Arg Ile Ala Val Ala Gly Lys Leu Leu Ser Glu Ile Thr Gly Ser
70 75 80 85
ttg ccc aac aag ccc gtt gat ttc cgc atc gac gga tcc aaa gct ttt 403
Leu Pro Asn Lys Pro Val Asp Phe Arg Ile Asp Gly Ser Lys Ala Phe
90 95 100
gtc act tgt ggt tct tcc cgc ttc gag ctg cca ctg atc ccg ctt gat 451
Val Thr Cys Gly Ser Ser Arg Phe Glu Leu Pro Leu Ile Pro Leu Asp
105 110 115
gat tac cca atg ctg ccc aag ctt cct gca ggt acc ggt tcc atc aac 499
Asp Tyr Pro Met Leu Pro Lys Leu Pro Ala Gly Thr Gly Ser Ile Asn
120 125 130
gcc aag ctg ttc acc gaa gca gtc tcc cag gtg gca tcc gct gca ggt 547
Ala Lys Leu Phe Thr Glu Ala Val Ser Gln Val Ala Ser Ala Ala Gly
135 140 145
aaa gat gat tct ttg ccg atg ctc acc ggt gtc agc atg gaa atc gtg 595
Lys Asp Asp Ser Leu Pro Met Leu Thr Gly Val Ser Met Glu Ile Val
150 155 160 165
ggc aac cag atc aac ttg gct gct act gac cgt ttc cgc ctg gcg ctg 643
Gly Asn Gln Ile Asn Lau Ala Ala Thr Asp Arg Phe Arg Leu Ala Leu
170 175 180
cgc acc ttc gaa tgg gaa gca aac gac cca gag ctc aac gtt aaa ctg 691
Arg Thr Phe Glu Trp Glu Ala Asn Asp Pro Glu Leu Asn Val Lys Leu
185 190 195
ctg att cct gcc cgc acc ctg ctg gac aac gcg cgt tct ttg gat tcc 739
Leu Ile Pro Ala Arg Thr Leu Leu Asp Asn Ala Arg Ser Leu Asp Ser
200 205 210
ggt ctc aac gat tcc atc gac atc gct gtc ggt acc ggc gat cag gtt 787
Gly Leu Asn Asp Ser Ile Asp Ile Ala Val Gly Thr Gly Asp Gln Val
215 220 225
ggc cgc gaa gga cta ttc ggc gtt cac acc gat aac cgc gaa acc acc 835
Gly Arg Glu Gly Leu Phe Gly Val His Thr Asp Asn Arg Glu Thr Thr
230 235 240 245
acc agg ctt ctc gac gcc gat ttc ccc aac atc gca cca ctg ctt ccc 883
Thr Arg Leu Leu Asp Ala Asp Phe Pro Asn Ile Ala Pro Leu Leu Pro
250 255 260
aaa gag cac acc gcg atc gca tca gtt gaa atc gca ccg ctg gtc gac 931
Lys Glu His Thr Ala Ile Ala Ser Val Glu Ile Ala Pro Leu Val Asp
265 270 275
gcc atc cgc cgc gta tcc ctg gtg gca gaa cgc aac gct cag atc gtc 979
Ala Ile Arg Arg Val Ser Leu Val Ala Glu Arg Asn Ala Gln Ile Val
280 285 290
ttg cac ttc agc gaa ggc cag gtc atc ttg act gca ggc gca acc gag 1027
Leu His Phe Ser Glu Gly Gln Val Ile Leu Thr Ala Gly Ala Thr Glu
295 300 305
gca ggc cac gcg gaa gaa acc ctg ccg tgt gca ttc acc ggc aag gaa 1075
Ala Gly His Ala Glu Glu Thr Leu Pro Cys Ala Phe Thr Gly Lys Glu
310 315 320 325
ctg acc atc gcg ttc aac cct ggc tac ctg aag gat gga ctt tct gta 1123
Leu Thr Ile Ala Phe Asn Pro Gly Tyr Leu Lys Asp Gly Leu Ser Val
330 335 340
gtt cca act tct cga gca gtc ttc gga ttc act gag cct tcc cgc cca 117l
Val Pro Thr Ser Arg Ala Val Phe Gly Phe Thr Glu Pro Ser Arg Pro
345 350 355
gcg atc atg atc cca gag cct gaa gaa atg cct tcc gcc aac gaa aat 1219
Ala Ile Met Ile Pro Glu Pro Glu Glu Met Pro Ser Ala Asn Glu Asn
360 365 370
ggc att ttc caa act ccg gac acc tat ttc acc tac ctg ctc atg cca 1267
Gly Ile Phe Gln Thr Pro Asp Thr Tyr Phe Thr Tyr Leu Leu Met Pro
375 380 385
gtg cgc ctg cca ggc taaacacaaa agtttcaccc ttttcgcgcc 1312
Val Arg Leu Pro Gly
390
<210>70
<211>394
<212>PRT
<213>谷氨酸棒状杆菌
<400>70
Met Glu Ser Gln Asn Val Ser Phe Arg Val Ala Arg Glu Asp Leu Val
1 5 10 15
Thr Ala Val Ala Trp Val Ala Arg Asn Leu Pro Thr Lys Pro Thr Gln
20 25 30
Pro Val Leu Arg Ala Met Leu Ile Thr Ala Asp Asp Glu Gly Leu Glu
35 40 45
Leu Ala Gly Tyr Asp Tyr Asp Val Ser Thr Arg Val Arg Leu Ser Ala
50 55 60
Glu Val Ser Gln Pro Gly Arg Ile Ala Val Ala Gly Lys Leu Leu Ser
65 70 75 80
Glu Ile Thr Gly Ser Leu Pro Asn Lys Pro Val Asp Phe Arg Ile Asp
85 90 95
Gly Ser Lys Ala Phe Val Thr Cys Gly Ser Ser Arg Phe Glu Leu Pro
100 105 110
Leu Ile Pro Leu Asp Asp Tyr Pro Met Leu Pro Lys Leu Pro Ala Gly
115 120 125
Thr Gly Ser Ile Asn Ala Lys Leu Phe Thr Glu Ala Val Ser Gln Val
130 135 140
Ala Ser Ala Ala Gly Lys Asp Asp Ser Leu Pro Met Leu Thr Gly Val
145 150 155 160
Ser Met Glu Ile Val Gly Asn Gln Ile Asn Leu Ala Ala Thr Asp Arg
165 170 175
Phe Arg Leu Ala Leu Arg Thr Phe Glu Trp Glu Ala Asn Asp Pro Glu
180 185 190
Leu Asn Val Lys Leu Leu Ile Pro Ala Arg Thr Leu Leu Asp Asn Ala
195 200 205
Arg Ser Leu Asp Ser Gly Leu Asn Asp Ser Ile Asp Ile Ala Val Gly
210 215 220
Thr Gly Asp Gln Val Gly Arg Glu Gly Leu Phe Gly Val His Thr Asp
225 230 235 240
Asn Arg Glu Thr Thr Thr Arg Leu Leu Asp Ala Asp Phe Pro Asn Ile
245 250 255
Ala Pro Leu Leu Pro Lys Glu His Thr Ala Ile Ala Ser Val Glu Ile
260 265 270
Ala Pro Leu Val Asp Ala Ile Arg Arg Val Ser Leu Val Ala Glu Arg
275 280 285
Asn Ala Gln Ile Val Leu His Phe Ser Glu Gly Gln Val Ile Leu Thr
290 295 300
Ala Gly Ala Thr Glu Ala Gly His Ala Glu Glu Thr Leu Pro Cys Ala
305 310 315 320
Phe Thr Gly Lys Glu Leu Thr Ile Ala Phe Asn Pro Gly Tyr Leu Lys
325 330 335
Asp Gly Leu Ser Val Val Pro Thr Ser Arg Ala Val Phe Gly Phe Thr
340 345 350
Glu Pro Ser Arg Pro Ala Ile Met Ile Pro Glu Pro Glu Glu Met Pro
355 360 365
Ser Ala Asn Glu Asn Gly Ile Phe Gln Thr Pro Asp Thr Tyr Phe Thr
370 375 380
Tyr Leu Leu Met Pro Val Arg Leu Pro Gly
385 390
<210>71
<211>331
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(301)
<223>RXA03332
<400>71
attctccttg cagcctataa gcgttaggct ttacctacac acccggaaca cccttacacc 60
agcgcgagct acagaagatt cacagattgg gagcccttcg atg aga ttc tat gtg 115
Met Arg Phe Tyr Val
1 5
acc aat agg gac acc gat cct ccc atg gga tcg agg gga acc tgg gtt 163
Thr Asn Arg Asp Thr Asp Pro Pro Met Gly Ser Arg Gly Thr Trp Val
10 15 20
aat cta ctc cgg gac aat tgg gat gac tat ggg ttc aaa act tct ttt 211
Asn Leu Leu Arg Asp Asn Trp Asp Asp Tyr Gly Phe Lys Thr Ser Phe
25 30 35
cat gta aaa cta tat cgg gac aat ggc gag aca ata cag ctc gga atg 259
His Val Lys Leu Tyr Arg Asp Asn Gly Glu Thr Ile Gln Leu Gly Met
40 45 50
gtg aag atc ctg cgt gcc gga caa att gag ttt tta ctg acc 301
Val Lys Ile Leu Arg Ala Gly Gln Ile Glu Phe Leu Leu Thr
55 60 65
taggggaccg actgaggaag tcgcacagtg 331
<210>72
<211>67
<212>PRT
<213>谷氨酸棒状杆菌
<400>72
Met Arg Phe Tyr Val Thr Asn Arg Asp Thr Asp Pro Pro Met Gly Ser
1 5 10 15
Arg Gly Thr Trp Val Asn Leu Leu Arg Asp Asn Trp Asp Asp Tyr Gly
20 25 30
Phe Lys Thr Ser Phe His Val Lys Leu Tyr Arg Asp Asn Gly Glu Thr
35 40 45
Ile Gln Leu Gly Met Val Lys Ile Leu Arg Ala Gly Gln Ile Glu Phe
50 55 60
Leu Leu Thr
65
<210>73
<211>1105
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1075)
<223>RXA03341
<400>73
taatgacgat ggaggtgtca agcagaatca tgaagtagcc caaaaggacg atgcttaaaa 60
tcatgtgact aatttttgat ggcagcaaga aaacgcaaca gtg atc gct tat gcc 115
Val Ile Ala Tyr Ala
1 5
ggt act act ggt acc tgt tta aaa gtc agc ccc aac gcc tat gtt gaa 163
Gly Thr Thr Gly Thr Cys Leu Lys Val Ser Pro Asn Ala Tyr Val Glu
10 15 20
gat atg gat gta cac gca gat act cga gaa ttc ctc act gcg cgc cgc 211
Asp Met Asp Val His Ala Asp Thr Arg Glu Phe Leu Thr Ala Arg Arg
25 30 35
aat cgt ctc act cca aaa gac gcc gga ttg cct att tac caa ggc aaa 259
Asn Arg Leu Thr Pro Lys Asp Ala Gly Leu Pro Ile Tyr Gln Gly Lys
40 45 50
agg cga gtt ccg ggg ctt cga cgt gaa gaa gtg gcc atg ttg gct ggc 307
Arg Arg Val Pro Gly Leu Arg Arg Glu Glu Val Ala Met Leu Ala Gly
55 60 65
gtg agc gtg gac tat tac aca cgt ctt gaa cga ggc aac ttg agt ggt 355
Val Ser Val Asp Tyr Tyr Thr Arg Leu Glu Arg Gly Asn Leu Ser Gly
70 75 80 85
gtg tca gaa cag gtg tta agc gcg tta gct act gcc tta cag ctg gat 403
Val Ser Glu Gln Val Leu Ser Ala Leu Ala Thr Ala Leu Gln Leu Asp
90 95 100
gat gcg gag acg cag cat ctg ttt gat ctg gcc aag ctc agt aat tca 451
Asp Ala Glu Thr Gln His Leu Phe Asp Leu Ala Lys Leu Ser Asn Ser
105 110 115
ccg gcg tca cgg cgt aag cgt acc cct gca ccg aag tct gtg ctg cgc 499
Pro Ala Ser Arg Arg Lys Arg Thr Pro Ala Pro Lys Ser Val Leu Arg
120 125 130
cct gag gtg ctg cga att ctc aat tcg atg cac gat att cca gca tat 547
Pro Glu Val Leu Arg Ile Leu Asn Ser Met His Asp Ile Pro Ala Tyr
135 140 145
att cgt tct gaa agc cgt gac ctt ctg gca gcc aac act ttt ggt cgt 595
Ile Arg Ser Glu Ser Arg Asp Leu Leu Ala Ala Asn Thr Phe Gly Arg
150 155 160 165
gcg cta tat gca ccg ttg tat gaa agc acg gtc tcc gat gga att agt 643
Ala Leu Tyr Ala Pro Leu Tyr Glu Ser Thr Val Ser Asp Gly Ile Ser
170 175 180
ggg gaa ccg ggg tca atc aat gtt gca cgc ttt aca ttc ctc gat cca 691
Gly Glu Pro Gly Ser Ile Asn Val Ala Arg Phe Thr Phe Leu Asp Pro
185 190 195
gca gcg cgc gaa ttt ttc cct gag tgg gag aga act tcg gca gat ttg 739
Ala Ala Arg Glu Phe Phe Pro Glu Trp Glu Arg Thr Ser Ala Asp Leu
200 205 210
gta gcc agc ttg cgc acc gtt gca gca cag cgg ccc aac gac acg ttg 787
Val Ala Ser Leu Arg Thr Val Ala Ala Gln Arg Pro Asn Asp Thr Leu
215 220 225
ttt agc aat ttg atc ggt gag ctg gtg acc aag tca gaa gtc ttc gcg 835
Phe Ser Asn Leu Ile Gly Glu Leu Val Thr Lys Ser Glu Val Phe Ala
230 235 240 245
caa atg tgg gcc gac cat aat gtc cgc atg cat cga aca ggt tcc aag 883
Gln Met Trp Ala Asp His Asn Val Arg Met His Arg Thr Gly Ser Lys
250 255 260
aag atc gtg cac ccg ttg gtg ggg gag atg gag ctg gat ttt gaa acg 931
Lys Ile Val His Pro Leu Val Gly Glu Met Glu Leu Asp Phe Glu Thr
265 270 275
ctt gat ctt cca gca gat ccc gat att gcc ctc gtg gtg tac tcc gct 979
Leu Asp Leu Pro Ala Asp Pro Asp Ile Ala Leu Val Val Tyr Ser Ala
280 285 290
gcg gaa ggt tcg aca tct gca atg aac ctt caa ctt ctg gcc aat tgg 1027
Ala Glu Gly Ser Thr Ser Ala Met Asn Leu Gln Leu Leu Ala Asn Trp
295 300 305
aca ggt aat gac agc ccc tcc ctc agc aca gaa ctt ggt gac act att 1075
Thr Gly Asn Asp Ser Pro Ser Leu Ser Thr Glu Leu Gly Asp Thr Ile
310 315 320 325
taattatgac tttcacactg aacaacggac 1105
<210>74
<211>325
<212>PRT
<213>谷氨酸棒状杆菌
<400>74
Val Ile Ala Tyr Ala Gly Thr Thr Gly Thr Cys Leu Lys Val Ser Pro
1 5 10 15
Asn Ala Tyr Val Glu Asp Met Asp Val His Ala Asp Thr Arg Glu Phe
20 25 30
Leu Thr Ala Arg Arg Asn Arg Leu Thr Pro Lys Asp Ala Gly Leu Pro
35 40 45
Ile Tyr Gln Gly Lys Arg Arg Val Pro Gly Leu Arg Arg Glu Glu Val
50 55 60
Ala Met Leu Ala Gly Val Ser Val Asp Tyr Tyr Thr Arg Leu Glu Arg
65 70 75 80
Gly Asn Leu Ser Gly Val Ser Glu Gln Val Leu Ser Ala Leu Ala Thr
85 90 95
Ala Leu Gln Leu Asp Asp Ala Glu Thr Gln His Leu Phe Asp Leu Ala
100 105 110
Lys Leu Ser Asn Ser Pro Ala Ser Arg Arg Lys Arg Thr Pro Ala Pro
115 120 125
Lys Ser Val Leu Arg Pro Glu Val Leu Arg Ile Leu Asn Ser Met His
130 135 140
Asp Ile Pro Ala Tyr Ile Arg Ser Glu Ser Arg Asp Leu Leu Ala Ala
145 150 155 160
Asn Thr Phe Gly Arg Ala Leu Tyr Ala Pro Leu Tyr Glu Ser Thr Val
165 170 175
Ser Asp Gly Ile Ser Gly Glu Pro Gly Ser Ile Asn Val Ala Arg Phe
180 185 190
Thr Phe Leu Asp Pro Ala Ala Arg Glu Phe Phe Pro Glu Trp Glu Arg
195 200 205
Thr Ser Ala Asp Leu Val Ala Ser Leu Arg Thr Val Ala Ala Gln Arg
210 215 220
Pro Asn Asp Thr Leu Phe Ser Asn Leu Ile Gly Glu Leu Val Thr Lys
225 230 235 240
Ser Glu Val Phe Ala Gln Met Trp Ala Asp His Asn Val Arg Met His
245 250 255
Arg Thr Gly Ser Lys Lys Ile Val His Pro Leu Val Gly Glu Met Glu
260 265 270
Leu Asp Phe Glu Thr Leu Asp Leu Pro Ala Asp Pro Asp Ile Ala Leu
275 280 285
Val Val Tyr Ser Ala Ala Glu Gly Ser Thr Ser Ala Met Asn Leu Gln
290 295 300
Leu Leu Ala Asn Trp Thr Gly Asn Asp Ser Pro Ser Leu Ser Thr Glu
305 310 315 320
Leu Gly Asp Thr Ile
325
<210>75
<211>1903
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(1873)
<223>RXA03590
<400>75
aaaagcaact cacttcgtgc ttttttcacc gaccccggct gtgtggatgc cacccactta 60
caccgcacaa tcgaaaataa aagtaaagga tgttcacctt atg tct gac acc aca 115
Met Ser Asp Thr Thr
1 5
gac agc cac gac cca gca acc tcc cct aca gtc gat tca gcc gca tcc 163
Asp Ser His Asp Pro Ala Thr Ser Pro Thr Val Asp Ser Ala Ala Ser
10 15 20
ggt act tct gac ccc aac cag caa ccc aag aag aaa ttc acc atc att 211
Gly Thr Ser Asp Pro Asn Gln Gln Pro Lys Lys Lys Phe Thr Ile Ile
25 30 35
aag cag gtg aac atg att gcg cgt act gcc att gat gac tgc att aat 259
Lys Gln Val Asn Met Ile Ala Arg Thr Ala Ile Asp Asp Cys Ile Asn
40 45 50
cga cta ggc cca gat gtt gtc acc atg tcc agc gaa gac att gct gat 307
Arg Leu Gly Pro Asp Val Val Thr Met Ser Ser Glu Asp Ile Ala Asp
55 60 65
caa gtg gtc aac tcc att aac gtt gct att gcc aga gaa aac aca cgt 355
Gln Val Val Asn Ser Ile Asn Val Ala Ile Ala Arg Glu Asn Thr Arg
70 75 80 85
gcc caa gat gcc aat gct gcg gtg cgt tac aca cct atc ttt aag ctc 403
Ala Gln Asp Ala Asn Ala Ala Val Arg Tyr Thr Pro Ile Phe Lys Leu
90 95 100
gat ttc tcc cat gtc gct gtc ctt atg cgc aga ctg cat gtg att atc 451
Asp Phe Ser His Val Ala Val Leu Met Arg Arg Leu His Val Ile Ile
105 110 115
aac atc gcg cca tcg cat aac tct gat cct gac agc gat atg ttg gct 499
Asn Ile Ala Pro Ser His Asn Ser Asp Pro Asp Ser Asp Met Leu Ala
120 125 130
att tac gac ccc aac cca cgc agc gag cat tac ggg atc tat cgc acc 547
Ile Tyr Asp Pro Asn Pro Arg Ser Glu His Tyr Gly Ile Tyr Arg Thr
135 140 145
tca gag gct gaa ata cgc cgc gta gcc cgt gaa tac tgc cct gac ctc 595
Ser Glu Ala Glu Ile Arg Arg Val Ala Arg Glu Tyr Cys Pro Asp Leu
150 155 160 165
acc tca gcg cag ttt aga gaa ctg cag atg gca ctc tca gat gct gca 643
Thr Ser Ala Gln Phe Arg Glu Leu Gln Met Ala Leu Ser Asp Ala Ala
170 175 180
cct cgt aaa gtc cgc cac aag cag cgt gac ttg att ccc gtg aaa aac 691
Pro Arg Lys Val Arg His Lys Gln Arg Asp Leu Ile Pro Val Lys Asn
185 190 195
ggc atc ttt aat tac tcg act aaa caa cta gaa ccg ttt tct caa gag 739
Gly Ile Phe Asn Tyr Ser Thr Lys Gln Leu Glu Pro Phe Ser Gln Glu
200 205 210
ttt gtc ttt ctt gct aaa tct gca gtg aac tac aac cca aat gca caa 787
Phe Val Phe Leu Ala Lys Ser Ala Val Asn Tyr Asn Pro Asn Ala Gln
215 220 225
aac cct gtc atc act cat ccc cag gat ggc tct gtc tgg gat gtt gaa 835
Asn Pro Val Ile Thr His Pro Gln Asp Gly Ser Val Trp Asp Val Glu
230 235 240 245
tca tgg atg aac gat ctc tcc gat gac cct gaa gtc gtt aac ctg ctg 883
Ser Trp Met Asn Asp Leu Ser Asp Asp Pro Glu Val Val Asn Leu Leu
250 255 260
tgg gag att atc ggc gct atc gtg cgc ccc tat gtg tcc tgg aac aag 931
Trp Glu Ile Ile Gly Ala Ile Val Arg Pro Tyr Val Ser Trp Asn Lys
265 270 275
tct gcc tgg ttc tac tca gag gcc ggc aac aac ggt aaa ggc acg ctc 979
Ser Ala Trp Phe Tyr Ser Glu Ala Gly Asn Asn Gly Lys Gly Thr Leu
280 285 290
gtt gag ctc atg cgc aac atc ttg ggc gca gag gcc tat acc agt atc 1027
Val Glu Leu Met Arg Asn Ile Leu Gly Ala Glu Ala Tyr Thr Ser Ile
295 300 305
cag ctc tcg gac ttc tct aaa gag ttc cac ctt gaa tca ctg act cgt 1075
Gln Leu Ser Asp Phe Ser Lys Glu Phe His Leu Glu Ser Leu Thr Arg
310 315 320 325
gca caa gca atc ctt gtt gat gag aac gat gtc ggc gcc ttc tta gaa 1123
Ala Gln Ala Ile Leu Val Asp Glu Asn Asp Val Gly Ala Phe Leu Glu
330 335 340
aag tca gca aac ctt aaa gcg att gtc acc aat gac gtc atc tcc att 1171
Lys Ser Ala Asn Leu Lys Ala Ile Val Thr Asn Asp Val Ile Ser Ile
345 350 355
aac cgc aag cac aaa acc atg ctc agc tat cag ttt tat ggc ttt atg 1219
Asn Arg Lys His Lys Thr Met Leu Ser Tyr Gln Phe Tyr Gly Phe Met
360 365 370
gtg cag tgt att aac ggc ttc ccc aag gtc aaa gac cag tcg gag tca 1267
Val Gln Cys Ile Asn Gly Phe Pro Lys Val Lys Asp Gln Ser Glu Ser
375 380 385
ttt ttc agg cgc caa ctc ttt gtg ccg ttt gag aaa agc ttc acc ggt 1315
Phe Phe Arg Arg Gln Leu Phe Val Pro Phe Glu Lys Ser Phe Thr Gly
390 395 400 405
gcc gag cgc aag tac atc aaa gac gac tac atg tcg cgc act gat gtc 1363
Ala Glu Arg Lys Tyr Ile Lys Asp Asp Tyr Met Ser Arg Thr Asp Val
410 415 420
ctc gaa tat gta ctg cac cgc gta ctg cat atg aac tac gac aat ctc 1411
Leu Glu Tyr Val Leu His Arg Val Leu His Met Asn Tyr Asp Asn Leu
425 430 435
tcc acc cct gct gca gct ctc gct gtg ctg gat gaa tac aaa gag ttt 1459
Ser Thr Pro Ala Ala Ala Leu Ala Val Leu Asp Glu Tyr Lys Glu Phe
440 445 450
gtc gat cct gtg cgc gca ttc tgg aat gaa ttc agc gat caa ttt gtc 1507
Val Asp Pro Val Arg Ala Phe Trp Asn Glu Phe Ser Asp Gln Phe Val
455 460 465
tgg gat ctt ctt ccc ctg caa ttc ctc tac gag ttt tat cgc aag tgg 1555
Trp Asp Leu Leu Pro Leu Gln Phe Leu Tyr Glu Phe Tyr Arg Lys Trp
470 475 480 485
ttt gac cgt gac agc cca tcc ggg tct gtg ctc ggc aaa cgc agc ttc 1603
Phe Asp Arg Asp Ser Pro Ser Gly Ser Val Leu Gly Lys Arg Ser Phe
490 495 500
att caa aaa atc acc acc atc gcc gtt gat agc ggt cag tgg gag tat 1651
Ile Gln Lys Ile Thr Thr Ile Ala Val Asp Ser Gly Gln Trp Glu Tyr
505 510 515
ccg ctg aca gca cag cgt ccc ggt ggc gat atg gcc gtt cct gag cca 1699
Pro Leu Thr Ala Gln Arg Pro Gly Gly Asp Met Ala Val Pro Glu Pro
520 525 530
tta gtc att gat tat gac ctc acc gag tgg caa aac gcc act gta cct 1747
Leu Val Ile Asp Tyr Asp Leu Thr Glu Trp Gln Asn Ala Thr Val Pro
535 540 545
aaa ggt cac gtc aac aag ggg ctc cct cta cca ctc aag gct aat tat 1795
Lys Gly His Val Asn Lys Gly Leu Pro Leu Pro Leu Lys Ala Asn Tyr
550 555 560 565
cga gga ctg ctc cgc aaa cct cta aca aca cct gct act ccc cct gca 1843
Arg Gly Leu Leu Arg Lys Pro Leu Thr Thr Pro Ala Thr Pro Pro Ala
570 575 580
gcg ccg ctc aac ccc aca cca ccc acc cct taacaaccac aagaaagatt 1893
Ala Pro Leu Asn Pro Thr Pro Pro Thr Pro
585 590
tatcatgtcc 1903
<210>76
<211>591
<212>PRT
<213>谷氨酸棒状杆菌
<400>76
Met Ser Asp Thr Thr Asp Ser His Asp Pro Ala Thr Ser Pro Thr Val
1 5 l0 15
Asp Ser Ala Ala Ser Gly Thr Ser Asp Pro Asn Gln Gln Pro Lys Lys
20 25 30
Lys Phe Thr Ile Ile Lys Gln Val Asn Met Ile Ala Arg Thr Ala Ile
35 40 45
Asp Asp Cys Ile Asn Arg Leu Gly Pro Asp Val Val Thr Met Ser Ser
50 55 60
Glu Asp Ile Ala Asp Gln Val Val Asn Ser Ile Asn Val Ala Ile Ala
65 70 75 80
Arg Glu Asn Thr Arg Ala Gln Asp Ala Asn Ala Ala Val Arg Tyr Thr
85 90 95
Pro Ile Phe Lys Leu Asp Phe Ser His Val Ala Val Leu Met Arg Arg
100 105 110
Leu His Val Ile Ile Asn Ile Ala Pro Ser His Asn Ser Asp Pro Asp
115 120 125
Ser Asp Met Leu Ala Ile Tyr Asp Pro Asn Pro Arg Ser Glu His Tyr
130 135 140
Gly Ile Tyr Arg Thr Ser Glu Ala Glu Ile Arg Arg Val Ala Arg Glu
145 150 155 160
Tyr Cys Pro Asp Leu Thr Ser Ala Gln Phe Arg Glu Leu Gln Met Ala
165 170 175
Leu Ser Asp Ala Ala Pro Arg Lys Val Arg His Lys Gln Arg Asp Leu
180 185 190
Ile Pro Val Lys Asn Gly Ile Phe Asn Tyr Ser Thr Lys Gln Leu Glu
195 200 205
Pro Phe Ser Gln Glu Phe Val Phe Leu Ala Lys Ser Ala Val Asn Tyr
210 215 220
Asn Pro Asn Ala Gln Asn Pro Val Ile Thr His Pro Gln Asp Gly Ser
225 230 235 240
Val Trp Asp Val Glu Ser Trp Met Asn Asp Leu Ser Asp Asp Pro Glu
245 250 255
Val Val Asn Leu Leu Trp Glu Ile Ile Gly Ala Ile Val Arg Pro Tyr
260 265 270
Val Ser Trp Asn Lys Ser Ala Trp Phe Tyr Ser Glu Ala Gly Asn Asn
275 280 285
Gly Lys Gly Thr Leu Val Glu Leu Met Arg Asn Ile Leu Gly Ala Glu
290 295 300
Ala Tyr Thr Ser Ile Gln Leu Ser Asp Phe Ser Lys Glu Phe His Leu
305 310 315 320
Glu Ser Leu Thr Arg Ala Gln Ala Ile Leu Val Asp Glu Asn Asp Val
325 330 335
Gly Ala Phe Leu Glu Lys Ser Ala Asn Leu Lys Ala Ile Val Thr Asn
340 345 350
Asp Val Ile Ser Ile Asn Arg Lys His Lys Thr Met Leu Ser Tyr Gln
355 360 365
Phe Tyr Gly Phe Met Val Gln Cys Ile Asn Gly Phe Pro Lys Val Lys
370 375 380
Asp Gln Ser Glu Ser Phe Phe Arg Arg Gln Leu Phe Val Pro Phe Glu
385 390 395 400
Lys Ser Phe Thr Gly Ala Glu Arg Lys Tyr Ile Lys Asp Asp Tyr Met
405 410 415
Ser Arg Thr Asp Val Leu Glu Tyr Val Leu His Arg Val Leu His Met
420 425 430
Asn Tyr Asp Asn Leu Ser Thr Pro Ala Ala Ala Leu Ala Val Leu Asp
435 440 445
Glu Tyr Lys Glu Phe Val Asp Pro Val Arg Ala Phe Trp Asn Glu Phe
450 455 460
Ser Asp Gln Phe Val Trp Asp Leu Leu Pro Leu Gln Phe Leu Tyr Glu
465 470 475 480
Phe Tyr Arg Lys Trp Phe Asp Arg Asp Ser Pro Ser Gly Ser Val Leu
485 490 495
Gly Lys Arg Ser Phe Ile Gln Lys Ile Thr Thr Ile Ala Val Asp Ser
500 505 510
Gly Gln Trp Glu Tyr Pro Leu Thr Ala Gln Arg Pro Gly Gly Asp Met
515 520 525
Ala Val Pro Glu Pro Leu Val Ile Asp Tyr Asp Leu Thr Glu Trp Gln
530 535 540
Asn Ala Thr Val Pro Lys Gly His Val Asn Lys Gly Leu Pro Leu Pro
545 550 555 560
Leu Lys Ala Asn Tyr Arg Gly Leu Leu Arg Lys Pro Leu Thr Thr Pro
565 570 575
Ala Thr Pro Pro Ala Ala Pro Leu Asn Pro Thr Pro Pro Thr Pro
580 585 590
<210>77
<211>994
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(964)
<223>RXA03607
<400>77
gggtaagaac ttacaggatc tcataggtca ggtgtttggg cgatggactg tcatagatcg 60
agcggaaagt agggtttcct ccacaggaag gaaaaccacc atg tgg cat tgc aga 115
Met Trp His Cys Arg
1 5
tgt act tgc ggt gtt gag aaa gat ctt gct gcg ggg tca ctg aag aat 163
Cys Thr Cys Gly Val Glu Lys Asp Leu Ala Ala Gly Ser Leu Lys Asn
10 15 20
ggt gat tcg aaa tcg tgt ggt tgt cta aaa atc gag tcg ctt tca aag 211
Gly Asp Ser Lys Ser Cys Gly Cys Leu Lys Ile Glu Ser Leu Ser Lys
25 30 35
aat agg gat tta gtt ggt tcc aca ttt ggt cga tgg tct gtt gtt cga 259
Asn Arg Asp Leu Val Gly Ser Thr Phe Gly Arg Trp Ser Val Val Arg
40 45 50
cgt ggt gag gac gtg ctg cag tgg ggt cgc tta tct aag gcg tgg ttt 307
Arg Gly Glu Asp Val Leu Gln Trp Gly Arg Leu Ser Lys Ala Trp Phe
55 60 65
tgc gag tgc gag tgt gga gcg aca agg gac gta ttg gaa cag tct ttg 355
Cys Glu Cys Glu Cys Gly Ala Thr Arg Asp Val Leu Glu Gln Ser Leu
70 75 80 85
ttg cgg ggt cgg tca ata tcg tgc ggg tgc tat cgc ata gaa cag cga 403
Leu Arg Gly Arg Ser Ile Ser Cys Gly Cys Tyr Arg Ile Glu Gln Arg
90 95 100
cga gaa act gct gtt ttt gaa gat ttg act ggc aag gta ttt aac cag 451
Arg Glu Thr Ala Val Phe Glu Asp Leu Thr Gly Lys Val Phe Asn Gln
105 110 115
tgg act gtg ctg tct cgt acg gat gac agg ttt tac cct ggt ggt ggg 499
Trp Thr Val Leu Ser Arg Thr Asp Asp Arg Phe Tyr Pro Gly Gly Gly
120 125 130
cgg tct caa atg tgg ttg tgc cga tgt atg tgc ggc tcc gaa aat gct 547
Arg Ser Gln Met Trp Leu Cys Arg Cys Met Cys Gly Ser Glu Asn Ala
135 140 145
gtt gct ggg att atg ttg agg cag ggt att agc caa tcg tgt ggt tgt 595
Val Ala Gly Ile Met Leu Arg Gln Gly Ile Ser Gln Ser Cys Gly Cys
150 155 160 165
ttg cac gga tca atc caa gag tca cat gtc agg gac ttt ctt tta aag 643
Leu His Gly Ser Ile Gln Glu Ser His Val Arg Asp Phe Leu Leu Lys
170 175 180
aat gat ttt cag ttt gaa tct caa aag act ttc gat ggt ttg gtg ggg 691
Asn Asp Phe Gln Phe Glu Ser Gln Lys Thr Phe Asp Gly Leu Val Gly
185 190 195
gtt ggt gga gga cca ctg agt tat gat ttt ttg gtt ttc aag gat acc 739
Val Gly Gly Gly Pro Leu Ser Tyr Asp Phe Leu Val Phe Lys Asp Thr
200 205 210
gag ccc tgg ttg ttg att gag tgc caa ggt gag cag cat ttt aga cca 787
Glu Pro Trp Leu Leu Ile Glu Cys Gln Gly Glu Gln His Phe Arg Pro
215 220 225
gtt gag tat ttc ggt gga gag agt cgt ttc gcg atc caa caa gag cac 835
Val Glu Tyr Phe Gly Gly Glu Ser Arg Phe Ala Ile Gln Gln Glu His
230 235 240 245
gac aga cga aaa cga gcg ttt act gta gtc cga gag cta aca ctt ttg 883
Asp Arg Arg Lys Arg Ala Phe Thr Val Val Arg Glu Leu Thr Leu Leu
250 255 260
gag atc cca tat acg gcg aga aca gca gaa tca att ggc gag tta tta 931
Glu Ile Pro Tyr Thr Ala Arg Thr Ala Glu Ser Ile Gly Glu Leu Leu
265 270 275
gta agt ggt ccg ttt cag gaa gaa gtt ttt gga tagcggtttt gcgtagactg 984
Val Ser Gly Pro Phe Gln Glu Glu Val Phe Gly
280 285
acgaagaata 994
<210>78
<211>288
<212>PRT
<213>谷氨酸棒状杆菌
<400>78
Met Trp His Cys Arg Cys Thr Cys Gly Val Glu Lys Asp Leu Ala Ala
1 5 10 15
Gly Ser Leu Lys Asn Gly Asp Ser Lys Ser Cys Gly Cys Leu Lys Ile
20 25 30
Glu Ser Leu Ser Lys Asn Arg Asp Leu Val Gly Ser Thr Phe Gly Arg
35 40 45
Trp Ser Val Val Arg Arg Gly Glu Asp Val Leu Gln Trp Gly Arg Leu
50 55 60
Ser Lys Ala Trp Phe Cys Glu Cys Glu Cys Gly Ala Thr Arg Asp Val
65 70 75 80
Leu Glu Gln Ser Leu Leu Arg Gly Arg Ser Ile Ser Cys Gly Cys Tyr
85 90 95
Arg Ile Glu Gln Arg Arg Glu Thr Ala Val Phe Glu Asp Leu Thr Gly
100 105 110
Lys Val Phe Asn Gln Trp Thr Val Leu Ser Arg Thr Asp Asp Arg Phe
115 120 125
Tyr Pro Gly Gly Gly Arg Ser Gln Met Trp Leu Cys Arg Cys Met Cys
130 135 140
Gly Ser Glu Asn Ala Val Ala Gly Ile Met Leu Arg Gln Gly Ile Ser
145 150 155 160
Gln Ser Cys Gly Cys Leu His Gly Ser Ile Gln Glu Ser His Val Arg
165 170 175
Asp Phe Leu Leu Lys Asn Asp Phe Gln Phe Glu Ser Gln Lys Thr Phe
180 185 190
Asp Gly Leu Val Gly Val Gly Gly Gly Pro Leu Ser Tyr Asp Phe Leu
195 200 205
Val Phe Lys Asp Thr Glu Pro Trp Leu Leu Ile Glu Cys Gln Gly Glu
210 215 220
Gln His Phe Arg Pro Val Glu Tyr Phe Gly Gly Glu Ser Arg Phe Ala
225 230 235 240
Ile Gln Gln Glu His Asp Arg Arg Lys Arg Ala Phe Thr Val Val Arg
245 250 255
Glu Leu Thr Leu Leu Glu Ile Pro Tyr Thr Ala Arg Thr Ala Glu Ser
260 265 270
Ile Gly Glu Leu Leu Val Ser Gly Pro Phe Gln Glu Glu Val Phe Gly
275 280 285
<210>79
<211>2407
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2377)
<223>RXA03743
<400>79
tgcgacccgc attgctgggt cggttgaata actcaaataa ctaattcaat tagtttcttg 60
accgtgttaa agtattaaaa aagtaagaaa ggttaatact ttg aca att gga att 115
Leu Thr Ile Gly Ile
1 5
ttg acg gag aaa gct tcg gct gcg aaa aac ttt gcg aaa gcc ctc ggt 163
Leu Thr Glu Lys Ala Ser Ala Ala Lys Asn Phe Ala Lys Ala Leu Gly
10 15 20
gga ccc tct ggc act tat aac ggc gag aag tac gtt att gct gca gcc 211
Gly Pro Ser Gly Thr Tyr Asn Gly Glu Lys Tyr Val Ile Ala Ala Ala
25 30 35
agc ggg cac ctt tat gaa ttt gtc gag cca gaa gag atg gtg cca cca 259
Ser Gly His Leu Tyr Glu Phe Val Glu Pro Glu Glu Met Val Pro Pro
40 45 50
agc tat gcc gat aag atc ggc ggc tac tgg gat ctg gct aag ctg ccg 307
Ser Tyr Ala Asp Lys Ile Gly Gly Tyr Trp Asp Leu Ala Lys Leu Pro
55 60 65
tgg gat gag acg cag ttt ttg tgg tca cgc gag caa aag cac ggt gca 355
Trp Asp Glu Thr Gln Phe Leu Trp Ser Arg Glu Gln Lys His Gly Ala
70 75 80 85
gct aac acg att gca cgc att aag aaa acc ttg agc agc tgc acc acc 403
Ala Asn Thr Ile Ala Arg Ile Lys Lys Thr Leu Ser Ser Cys Thr Thr
90 95 100
att gtg atc gga tca gat att gat ccc acg ggc gaa ggt gat ctg ttg 451
Ile Val Ile Gly Ser Asp Ile Asp Pro Thr Gly Glu Gly Asp Leu Leu
105 110 115
gcg tgg gag att atc gct gag ctg ggg tta gac acc aag aag att cag 499
Ala Trp Glu Ile Ile Ala Glu Leu Gly Leu Asp Thr Lys Lys Ile Gln
120 125 130
cgt atg gaa ttt gtt gat gag gcg ccg gcc tcg att cag cag gcg ttt 547
Arg Met Glu Phe Val Asp Glu Ala Pro Ala Ser Ile Gln Gln Ala Phe
135 140 145
atc aac cgc cgc gat gtg acc tcc atg cat gac gag ccc aat tat ctt 595
Ile Asn Arg Arg Asp Val Thr Ser Met His Asp Glu Pro Asn Tyr Leu
150 155 160 165
aag gcg gaa ttc cga tca cgc ttt gat ttg ctg tcg atg cag tgg act 643
Lys Ala Glu Phe Arg Ser Arg Phe Asp Leu Leu Ser Met Gln Trp Thr
170 175 180
cgc ggt gcc acc aaa gtg cta gag acc act ggt cgg cgt gca gtg ttg 691
Arg Gly Ala Thr Lys Val Leu Glu Thr Thr Gly Arg Arg Ala Val Leu
185 190 195
cgc aat ggt cgc ctg aaa tct gcc atg gtt gcg att gtg ggg cag ggc 739
Arg Asn Gly Arg Leu Lys Ser Ala Met Val Ala Ile Val Gly Gln Gly
200 205 210
tta gat gcg tac aac gat tat aaa aag att cct ttt tat caa aac cgc 787
Leu Asp Ala Tyr Asn Asp Tyr Lys Lys Ile Pro Phe Tyr Gln Asn Arg
215 220 225
ttc att gat gat cac ggt gta agt tac gtc aac ccc gag gaa ccg cgt 835
Phe Ile Asp Asp His Gly Val Ser Tyr Val Asn Pro Glu Glu Pro Arg
230 235 240 245
tat aag act tgc gac gag gta cca cag ctt tat aag gct agt gcg gtc 883
Tyr Lys Thr Cys Asp Glu Val Pro Gln Leu Tyr Lys Ala Ser Ala Val
250 255 260
gaa tgc tat gag aag agc atg aaa aag acc gcg cca cca cgt cta ctt 931
Glu Cys Tyr Glu Lys Ser Met Lys Lys Thr Ala Pro Pro Arg Leu Leu
265 270 275
gac ctg gcg agc ctg tcg gcc ctg ctg tct aaa gag ggc ttt agc gca 979
Asp Leu Ala Ser Leu Ser Ala Leu Leu Ser Lys Glu Gly Phe Ser Ala
280 285 290
aag aac gtg ctt aag act tat cag cag atg tac gag gcg cag gtc gtg 1027
Lys Asn Val Leu Lys Thr Tyr Gln Gln Met Tyr Glu Ala Gln Val Val
295 300 305
tct tat ccg cgt acc gag gat aag acg att acc cct gag cag ttt aag 1075
Ser Tyr Pro Arg Thr Glu Asp Lys Thr Ile Thr Pro Glu Gln Phe Lys
310 315 320 325
gaa cta gcg ccg ttg gtc gat aag atc gcc ggc ctg gtt ggt gtg aac 1123
Glu Leu Ala Pro Leu Val Asp Lys Ile Ala Gly Leu Val Gly Val Asn
330 335 340
ccg gct gac ctt acg cac cga cag cca cgg tcc acg cat gtt aag ccc 1171
Pro Ala Asp Leu Thr His Arg Gln Pro Arg Ser Thr His Val Lys Pro
345 350 355
aag ggt gca cac ggc gcc aac cgt ccg ggt ttg aat gtg ccg acc agt 1219
Lys Gly Ala His Gly Ala Asn Arg Pro Gly Leu Asn Val Pro Thr Ser
360 365 370
att gca gcg gtg aaa acc acc tac ggt gtg ctc gga cag cgc att tat 1267
Ile Ala Ala Val Lys Thr Thr Tyr Gly Val Leu Gly Gln Arg Ile Tyr
375 380 385
gaa gtg ctc gct aag agc tat ctc acg atg ctg gca gag gat tac ctc 1315
Glu Val Leu Ala Lys Ser Tyr Leu Thr Met Leu Ala Glu Asp Tyr Leu
390 395 400 405
tat gag cac caa aaa ggc cgc gtc gtg gat tac cca gcg ttt ctc ggt 1363
Tyr Glu His Gln Lys Gly Arg Val Val Asp Tyr Pro Ala Phe Leu Gly
410 415 420
aca gca aat gtg cca aag tcc ttg ggt tgg aaa ggt att ttc gac gta 1411
Thr Ala Asn Val Pro Lys Ser Leu Gly Trp Lys Gly Ile Phe Asp Val
425 430 435
gac gct gag gct gat gat gat gct gcc ggc agt gat gca gca cag cag 1459
Asp Ala Glu Ala Asp Asp Asp Ala Ala Gly Ser Asp Ala Ala Gln Gln
440 445 450
ggg att ggt act cgt gct gag cca gaa gtg ttt gag ggt ttt ccg cag 1507
Gly Ile Gly Thr Arg Ala Glu Pro Glu Val Phe Glu Gly Phe Pro Gln
455 460 465
cga cca ccg cat ccg tca atg acg tgg ttg atg aaa cag ctc gat aag 1555
Arg Pro Pro His Pro Ser Met Thr Trp Leu Met Lys Gln Leu Asp Lys
470 475 480 485
cat gat gtc ggt acc ggt gcg aca cga acg agc acc tat gcc gag gtc 1603
His Asp Val Gly Thr Gly Ala Thr Arg Thr Ser Thr Tyr Ala Glu Val
490 495 500
aca gcc ggc aag tca gca ttg ctc aaa gaa acg cgt ggc aaa gtc act 1651
Thr Ala Gly Lys Ser Ala Leu Leu Lys Glu Thr Arg Gly Lys Val Thr
505 510 515
atg act gac gca ggg caa ctg aat tat ctg ctg ctt cct ggc acc cac 1699
Met Thr Asp Ala Gly Gln Leu Asn Tyr Leu Leu Leu Pro Gly Thr His
520 525 530
att ggc gac ctc aaa ctt acc gag cgc gtt tat gcc gat atg gca gcg 1747
Ile Gly Asp Leu Lys Leu Thr Glu Arg Val Tyr Ala Asp Met Ala Ala
535 540 545
gtt gct gct ggt gaa aag act gca gag cag gca ctg gaa cca gtt aaa 1795
Val Ala Ala Gly Glu Lys Thr Ala Glu Gln Ala Leu Glu Pro Val Lys
550 555 560 565
cag tgg gtc gaa gaa gat att gcg acc atg acc cgt aac gca aaa aac 1843
Gln Trp Val Glu Glu Asp Ile Ala Thr Met Thr Arg Asn Ala Lys Asn
570 575 580
atc ccc caa gaa cta gga aag aaa ctc atg act act act ttt acc cct 1891
Ile Pro Gln Glu Leu Gly Lys Lys Leu Met Thr Thr Thr Phe Thr Pro
585 590 595
tct gtc aag cac tcc ggc acc tgg aac ggt gag cag gtg agc ttt aag 1939
Ser Val Lys His Ser Gly Thr Trp Asn Gly Glu Gln Val Ser Phe Lys
600 605 610
aag gtc tac tgc ggg cat gaa ttt agc gat gca gaa tgc gag gcg ttg 1987
Lys Val Tyr Cys Gly His Glu Phe Ser Asp Ala Glu Cys Glu Ala Leu
615 620 625
ctg cgc ggt gag gag ctc acc att acc gat gcc atg att ggt gga cag 2035
Leu Arg Gly Glu Glu Leu Thr Ile Thr Asp Ala Met Ile Gly Gly Gln
630 635 640 645
atc acg aca gtc acc ggc aag ctg gcg cat caa agc ttt aac aaa gac 2083
Ile Thr Thr Val Thr Gly Lys Leu Ala His Gln Ser Phe Asn Lys Asp
650 655 660
ggc aag acc att aag ttc ttt ggg ttt aag ggg cag gtt gat cgc att 2131
Gly Lys Thr Ile Lys Phe Phe Gly Phe Lys Gly Gln Val Asp Arg Ile
665 670 675
gcc tct gca gac cca gct gtg tac gcg gtg ggt gtg tgg aag gtt gag 2179
Ala Ser Ala Asp Pro Ala Val Tyr Ala Val Gly Val Trp Lys Val Glu
680 685 690
ggt gaa aag att cgc ttc aag cgc gta tgg ggt ggt cac aag ttc acc 2227
Gly Glu Lys Ile Arg Phe Lys Arg Val Trp Gly Gly His Lys Phe Thr
695 700 705
gac aag gag atc gcc gat ctg ctt gag ggc cgc gag att gct ttt gat 2275
Asp Lys Glu Ile Ala Asp Leu Leu Glu Gly Arg Glu Ile Ala Phe Asp
710 715 720 725
gcc atg tcg aag gcc aag aag ccg tac acc gct cgg gga tcg ctg cag 2323
Ala Met Ser Lys Ala Lys Lys Pro Tyr Thr Ala Arg Gly Ser Leu Gln
730 735 740
cgc ggc gag tac aac ggt aat tcg ttc gtc ggc ttc cag ctc gca cca 2371
Arg Gly Glu Tyr Asn Gly Asn Ser Phe Val Gly Phe Gln Leu Ala Pro
745 750 755
cgc gat taacgcacaa gtacgtgcac tagaagaaac 2407
Arg Asp
<210>80
<211>759
<212>PRT
<213>谷氨酸棒状杆菌
<400>80
Leu Thr Ile Gly Ile Leu Thr Glu Lys Ala Ser Ala Ala Lys Asn Phe
1 5 10 15
Ala Lys Ala Leu Gly Gly Pro Ser Gly Thr Tyr Asn Gly Glu Lys Tyr
20 25 30
Val Ile Ala Ala Ala Ser Gly His Leu Tyr Glu Phe Val Glu Pro Glu
35 40 45
Glu Met Val Pro Pro Ser Tyr Ala Asp Lys Ile Gly Gly Tyr Trp Asp
50 55 60
Leu Ala Lys Leu Pro Trp Asp Glu Thr Gln Phe Leu Trp Ser Arg Glu
65 70 75 80
Gln Lys His Gly Ala Ala Asn Thr Ile Ala Arg Ile Lys Lys Thr Leu
85 90 95
Ser Ser Cys Thr Thr Ile Val Ile Gly Ser Asp Ile Asp Pro Thr Gly
100 105 110
Glu Gly Asp Leu Leu Ala Trp Glu Ile Ile Ala Glu Leu Gly Leu Asp
115 120 125
Thr Lys Lys Ile Gln Arg Met Glu Phe Val Asp Glu Ala Pro Ala Ser
130 135 140
Ile Gln Gln Ala Phe Ile Asn Arg Arg Asp Val Thr Ser Met His Asp
145 150 155 160
Glu Pro Asn Tyr Leu Lys Ala Glu Phe Arg Ser Arg Phe Asp Leu Leu
165 170 175
Ser Met Gln Trp Thr Arg Gly Ala Ihr Lys Val Leu Glu Thr Thr Gly
180 185 190
Arg Arg Ala Val Leu Arg Asn Gly Arg Leu Lys Ser Ala Met Val Ala
195 200 205
Ile Val Gly Gln Gly Leu Asp Ala Tyr Asn Asp Tyr Lys Lys Ile Pro
210 215 220
Phe Tyr Gln Asn Arg Phe Ile Asp Asp His Gly Val Ser Tyr Val Asn
225 230 235 240
Pro Glu Glu Pro Arg Tyr Lys Thr Cys Asp Glu Val Pro Gln Leu Tyr
245 250 255
Lys Ala Ser Ala Val Glu Cys Tyr Glu Lys Ser Met Lys Lys Thr Ala
260 265 270
Pro Pro Arg Leu Leu Asp Leu Ala Ser Leu Ser Ala Leu Leu Ser Lys
275 280 285
Glu Gly Phe Ser Ala Lys Asn Val Leu Lys Thr Tyr Gln Gln Met Tyr
290 295 300
Glu Ala Gln Val Val Ser Tyr Pro Arg Thr Glu Asp Lys Thr Ile Thr
305 310 315 320
Pro Glu Gln Phe Lys Glu Leu Ala Pro Leu Val Asp Lys Ile Ala Gly
325 330 335
Leu Val Gly Val Asn Pro Ala Asp Leu Thr His Arg Gln Pro Arg Ser
340 345 350
Thr His Val Lys Pro Lys Gly Ala His Gly Ala Asn Arg Pro Gly Leu
355 360 365
Asn Val Pro Thr Ser Ile Ala Ala Val Lys Thr Thr Tyr Gly Val Leu
370 375 380
Gly Gln Arg Ile Tyr Glu Val Leu Ala Lys Ser Tyr Leu Thr Met Leu
385 390 395 400
Ala Glu Asp Tyr Leu Tyr Glu His Gln Lys Gly Arg Val Val Asp Tyr
405 410 415
Pro Ala Phe Leu Gly Thr Ala Asn Val Pro Lys Ser Leu Gly Trp Lys
420 425 430
Gly Ile Phe Asp Val Asp Ala Glu Ala Asp Asp Asp Ala Ala Gly Ser
435 440 445
Asp Ala Ala Gln Gln Gly Ile Gly Thr Arg Ala Glu Pro Glu Val Phe
450 455 460
Glu Gly Phe Pro Gln Arg Pro Pro His Pro Ser Met Thr Trp Leu Met
465 470 475 480
Lys Gln Leu Asp Lys His Asp Val Gly Thr Gly Ala Thr Arg Thr Ser
485 490 495
Thr Tyr Ala Glu Val Thr Ala Gly Lys Ser Ala Leu Leu Lys Glu Thr
500 505 510
Arg Gly Lys Val Thr Met Thr Asp Ala Gly Gln Leu Asn Tyr Leu Leu
515 520 525
Leu Pro Gly Thr His Ile Gly Asp Leu Lys Leu Thr Glu Arg Val Tyr
530 535 540
Ala Asp Met Ala Ala Val Ala Ala Gly Glu Lys Thr Ala Glu Gln Ala
545 550 555 560
Leu Glu Pro Val Lys Gln Trp Val Glu Glu Asp Ile Ala Thr Met Thr
565 570 575
Arg Asn Ala Lys Asn Ile Pro Gln Glu Leu Gly Lys Lys Leu Met Thr
580 585 590
Thr Thr Phe Thr Pro Ser Val Lys His Ser Gly Thr Trp Asn Gly Glu
595 600 605
Gln Val Ser Phe Lys Lys Val Tyr Cys Gly His Glu Phe Ser Asp Ala
610 615 620
Glu Cys Glu Ala Leu Leu Arg Gly Glu Glu Leu Thr Ile Thr Asp Ala
625 630 635 640
Met Ile Gly Gly Gln Ile Thr Thr Val Thr Gly Lys Leu Ala His Gln
645 650 655
Ser Phe Asn Lys Asp Gly Lys Thr Ile Lys Phe Phe Gly Phe Lys Gly
660 665 670
Gln Val Asp Arg Ile Ala Ser Ala Asp Pro Ala Val Tyr Ala Val Gly
675 680 685
Val Trp Lys Val Glu Gly Glu Lys Ile Arg Phe Lys Arg Val Trp Gly
690 695 700
Gly His Lys Phe Thr Asp Lys Glu Ile Ala Asp Leu Leu Glu Gly Arg
705 710 715 720
Glu Ile Ala Phe Asp Ala Met Ser Lys Ala Lys Lys Pro Tyr Thr Ala
725 730 735
Arg Gly Ser Leu Gln Arg Gly Glu Tyr Asn Gly Asn Ser Phe Val Gly
740 745 750
Phe Gln Leu Ala Pro Arg Asp
755
<210>81
<211>3118
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(3088)
<223>RXA03903
<400>81
catagtgggc acagacacgc aataagaaca gtggctttct gaagtgttct aaaaggaaca 60
tgaagggggt caaggcagtt aacgagaggg atgcattccc gtg gca gat acc gca 115
Val Ala Asp Thr Ala
1 5
ggc acc aca gga tcg aaa aag aag tac ttg gtg atc gtc gag tcg gcg 163
Gly Thr Thr Gly Ser Lys Lys Lys Tyr Leu Val Ile Val Glu Ser Ala
10 15 20
acc aag gct aaa aag att cag cct tac ctt ggc aac gac tac atc gtc 211
Thr Lys Ala Lys Lys Ile Gln Pro Tyr Leu Gly Asn Asp Tyr Ile Val
25 30 35
gag gcc tcc gtt ggt cat att cgt gat ctg cca cgt ggc gct gct gac 259
Glu Ala Ser Val Gly His Ile Arg Asp Leu Pro Arg Gly Ala Ala Asp
40 45 50
atc cct gca aag tac aag aag gag cct tgg gct cgt ctt ggt gtg gac 307
Ile Pro Ala Lys Tyr Lys Lys Glu Pro Trp Ala Arg Leu Gly Val Asp
55 60 65
acc gat cgc ggt ttc gcg ccg ctt tat gtg gtg agc ccc gat aaa aag 355
Thr Asp Arg Gly Phe Ala Pro Leu Tyr Val Val Ser Pro Asp Lys Lys
70 75 80 85
aag aag gtc gct gac ctc aag gcg aag ctc aag ctc gtt gat gag ttg 403
Lys Lys Val Ala Asp Leu Lys Ala Lys Leu Lys Leu Val Asp Glu Leu
90 95 100
ctg ctg gca aca gac ccc gac cgt gag ggc gag gcg att gcg tgg cat 451
Leu Leu Ala Thr Asp Pro Asp Arg Glu Gly Glu Ala Ile Ala Trp His
105 110 115
ttg ctt gag gtg ttg aag ccg act gtt cct gtg cgt cgc atg gtg ttc 499
Leu Leu Glu Val Leu Lys Pro Thr Val Pro Val Arg Arg Met Val Phe
120 125 130
aat gag atc acg aag cct gcc att ttg gct gcg gcg gaa aac act cgt 547
Asn Glu Ile Thr Lys Pro Ala Ile Leu Ala Ala Ala Glu Asn Thr Arg
135 140 145
gag ctg gat gag aac ctg gtg gat gcg cag gaa act cgt cgt att ctg 595
Glu Leu Asp Glu Asn Leu Val Asp Ala Gln Glu Thr Arg Arg Ile Leu
150 155 160 165
gac cgt ttg tac ggc tat gaa gtc tct cct gtg ctg tgg aaa aag gtc 643
Asp Arg Leu Tyr Gly Tyr Glu Val Ser Pro Val Leu Trp Lys Lys Val
170 175 180
atg ccg agg ttg tcg gcg ggc cgt gtg cag tcg gtg gca acc cgt gtg 691
Met Pro Arg Leu Ser Ala Gly Arg Val Gln Ser Val Ala Thr Arg Val
185 190 195
att gtt gag cgg gag cgc gag cgc atg gcg ttc gtg tcg gcg gat tat 739
Ile Val Glu Arg Glu Arg Glu Arg Met Ala Phe Val Ser Ala Asp Tyr
200 205 210
tgg gat ctg tcg gcg gag ttt aat gcg cgt gaa aac ggc aag gcg gat 787
Trp Asp Leu Ser Ala Glu Phe Asn Ala Arg Glu Asn Gly Lys Ala Asp
215 220 225
tcg gat aac ccg tcg tcg ttt act gcg cgt ttg tcc acg att gat gga 835
Ser Asp Asn Pro Ser Ser Phe Thr Ala Arg Leu Ser Thr Ile Asp Gly
230 235 240 245
aac cgt gtt gct caa ggc cgt gat ttt aat gat cgg gga gag ctg acc 883
Asn Arg Val Ala Gln Gly Arg Asp Phe Asn Asp Arg Gly Glu Leu Thr
250 255 260
tcg gag gct gtc gtc gtc gat aag cag cgt gct gag gcg tta gcc gag 931
Ser Glu Ala Val Val Val Asp Lys Gln Arg Ala Glu Ala Leu Ala Glu
265 270 275
gct ttg gaa ggc cag gaa atg gcc gtc gtt ggg gtc gag gaa aag ccg 979
Ala Leu Glu Gly Gln Glu Met Ala Val Val Gly Val Glu Glu Lys Pro
280 285 290
tac acc cgt cgc cct tat gcg ccg ttt atg acc tct acg ctg cag caa 1027
Tyr Thr Arg Arg Pro Tyr Ala Pro Phe Met Thr Ser Thr Leu Gln Gln
295 300 305
gag tct ggc cgc aag ctg cat tac act tct gag cgc acg atg cgt att 1075
Glu Ser Gly Arg Lys Leu His Tyr Thr Ser Glu Arg Thr Met Arg Ile
310 315 320 325
gcg cag cgc ttg tat gaa aac ggc cat atc act tat atg cgt act gac 1123
Ala Gln Arg Leu Tyr Glu Asn Gly His Ile Thr Tyr Met Arg Thr Asp
330 335 340
tcg acc tcg ttg tcg gag cag ggc atg aag gct gcg cgc gat cag gcg 1171
Ser Thr Ser Leu Ser Glu Gln Gly Met Lys Ala Ala Arg Asp Gln Ala
345 350 355
ttg gag ctg tac ggt gcg gaa tat gtt tcg ccg agc cca cgt acc tat 1219
Leu Glu Leu Tyr Gly Ala Glu Tyr Val Ser Pro Ser Pro Arg Thr Tyr
360 365 370
gac cgc aag gtg aag aac tcc cag gag gcc cac gag gcg att cgc cca 1267
Asp Arg Lys Val Lys Asn Ser Gln Glu Ala His Glu Ala Ile Arg Pro
375 380 385
gct ggt gaa act ttt gcg acc ccg ggc cag ctg cat ggc cag ttg gat 1315
Ala Gly Glu Thr Phe Ala Thr Pro Gly Gln Leu His Gly Gln Leu Asp
390 395 400 405
gcg gaa gaa ttt aag ctc tat gag ctg att tgg cag cgc act gtg gcc 1363
Ala Glu Glu Phe Lys Leu Tyr Glu Leu Ile Trp Gln Arg Thr Val Ala
410 415 420
tcc cag atg gcc gat gcc aag ggc acg tcc atg aag gtc acc atc ggt 1411
Ser Gln Met Ala Asp Ala Lys Gly Thr Ser Met Lys Val Thr Ile Gly
425 430 435
ggc acc gcg aag acc ggc gag aag act gag ttc aac gcg acc ggc cgc 1459
Gly Thr Ala Lys Thr Gly Glu Lys Thr Glu Phe Asn Ala Thr Gly Arg
440 445 450
acg ctg act ttc cct ggc ttc ctg cgc gct tac gtg gaa acc acc cgc 1507
Thr Leu Thr Phe Pro Gly Phe Leu Arg Ala Tyr Val Glu Thr Thr Arg
455 460 465
acc gcc gat ggc cgc gac gta gct gac aac gcc gaa aag cgt ctg cca 1555
Thr Ala Asp Gly Arg Asp Val Ala Asp Asn Ala Glu Lys Arg Leu Pro
470 475 480 485
ctg ctg tct gag ggc gat ctg ctc aag gtt ttg agc atc gaa gcc gat 1603
Leu Leu Ser Glu Gly Asp Leu Leu Lys Val Leu Ser Ile Glu Ala Asp
490 495 500
ggt cac agc acc aat cca cct gcg cgc tac aca gag gcg tcg ctg gtg 1651
Gly His Ser Thr Asn Pro Pro Ala Arg Tyr Thr Glu Ala Ser Leu Val
505 510 515
aag aag atg gaa gat ctg ggc atc ggc cgt cct tcc act tat gca tcg 1699
Lys Lys Met Glu Asp Leu Gly Ile Gly Arg Pro Ser Thr Tyr Ala Ser
520 525 530
atc att aag acg att cag gat cga ggc tac gtt tat tcg cgt ggc aat 1747
Ile Ile Lys Thr Ile Gln Asp Arg Gly Tyr Val Tyr Ser Arg Gly Asn
535 540 545
gcg ctg gtg ccg tcc tgg gtc gcg ttc gcc gtg gtc gga ttg ctt gaa 1795
Ala Leu Val Pro Ser Trp Val Ala Phe Ala Val Val Gly Leu Leu Glu
550 555 560 565
gcc aac ttc acc tcg ctg gtg gat tac gat ttc acc tcc tcc atg gaa 1843
Ala Asn Phe Thr Ser Leu Val Asp Tyr Asp Phe Thr Ser Ser Met Glu
570 575 580
gat gag ctg gac aac atc gcc gca ggt cgc gag ggc cgc acg gag tgg 1891
Asp Glu Leu Asp Asn Ile Ala Ala Gly Arg Glu Gly Arg Thr Glu Trp
585 590 595
ctc aac ggt ttc tac ttc ggc gat gcc gaa gcg gat cag tcc atg gct 1939
Leu Asn Gly Phe Tyr Phe Gly Asp Ala Glu Ala Asp Gln Ser Met Ala
600 605 610
gaa tca gtt gcc cgc cag ggc ggt ttg aag gcg ctt gtc gac gcg aac 1987
Glu Ser Val Ala Arg Gln Gly Gly Leu Lys Ala Leu Val Asp Ala Asn
615 620 625
ctg gag cac atc gac gcg cgt tca gta aac tca ctc aag ctt ttc gac 2035
Leu Glu His Ile Asp Ala Arg Ser Val Asn Ser Leu Lys Leu Phe Asp
630 635 640 645
gac gcc gaa ggc cgt gcc gtg aac gtt cga gtc gga cgc tac ggt ccg 2083
Asp Ala Glu Gly Arg Ala Val Asn Val Arg Val Gly Arg Tyr Gly Pro
650 655 660
tac atc gag cgc atc gtg ggc acc acc gcg gaa ggc gag cca gaa ttt 2131
Tyr Ile Glu Arg Ile Val Gly Thr Thr Ala Glu Gly Glu Pro Glu Phe
665 670 675
cag cgc gcc aac cta cct gag gaa acc acg cct gat gag ctg acc ctc 2179
Gln Arg Ala Asn Leu Pro Glu Glu Thr Thr Pro Asp Glu Leu Thr Leu
680 685 690
gag gtc gct gag aag ctc ttc gct acc cca caa ggt gga cgt gaa ctg 2227
Glu Val Ala Glu Lys Leu Phe Ala Thr Pro Gln Gly Gly Arg Glu Leu
695 700 705
ggc att aac cca gca aac ggt cgc atg gtg gtg gct aag gaa ggc cgc 2275
Gly Ile Asn Pro Ala Asn Gly Arg Met Val Val Ala Lys Glu Gly Arg
710 715 720 725
ttt ggt cca tac gtg atc gag cag gtc acg gac tca gag cgc gct ggc 2323
Phe Gly Pro Tyr Val Ile Glu Gln Val Thr Asp Ser Glu Arg Ala Gly
730 735 740
gcc gaa gcc caa gca gaa gaa gtc gtt gca gcg gaa cga aaa gct gaa 2371
Ala Glu Ala Gln Ala Glu Glu Val Val Ala Ala Glu Arg Lys Ala Glu
745 750 755
gac gaa caa cgt gcc acc gat gga atg cga ccc aag aac tgg gaa acc 2419
Asp Glu Gln Arg Ala Thr Asp Gly Met Arg Pro Lys Asn Trp Glu Thr
760 765 770
aag act gcc gca aac cag aag gaa aag cgc atc aac cag ctg gtt gag 2467
Lys Thr Ala Ala Asn Gln Lys Glu Lys Arg Ile Asn Gln Leu Val Glu
775 780 785
gaa aac ctc aag cca gcg acc gca tcc ctg ttc agc ggc atg gaa cct 2515
Glu Asn Leu Lys Pro Ala Thr Ala Ser Leu Phe Ser Gly Met Glu Pro
790 795 800 805
gca acc gtg acc ctg gaa gaa gcc ctc aag ctg ctg tcc ctg cca cgc 2563
Ala Thr Val Thr Leu Glu Glu Ala Leu Lys Leu Leu Ser Leu Pro Arg
810 815 820
gaa gta ggt gtc gat cct tcc gac aac gaa gtg atc acc gct caa aac 2611
Glu Val Gly Val Asp Pro Ser Asp Asn Glu Val Ile Thr Ala Gln Asn
825 830 835
gga cga tac ggc cct tat ctg aag aag ggt agc gac tcc cgt tcc ctc 2659
Gly Arg Tyr Gly Pro Tyr Leu Lys Lys Gly Ser Asp Ser Arg Ser Leu
840 845 850
aac agc gaa gag cag atc ttc acc gtc act ttg gat gag gct cgc cgc 2707
Asn Ser Glu Glu Gln Ile Phe Thr Val Thr Leu Asp Glu Ala Arg Arg
855 860 865
atc tac gcc gaa cca aag cgt cgt gga cgc gcc gct gct cag cca cca 2755
Ile Tyr Ala Glu Pro Lys Arg Arg Gly Arg Ala Ala Ala Gln Pro Pro
870 875 880 885
ctg aag caa ctt ggc gac aat gac gtt tcc ggc aaa cca atg acc gtc 2803
Leu Lys Gln Leu Gly Asp Asn Asp Val Ser Gly Lys Pro Met Thr Val
890 895 900
aag gac gga cgt ttc ggc cca tac gtc acc gac ggc acc acc aac gcg 2851
Lys Asp Gly Arg Phe Gly Pro Tyr Val Thr Asp Gly Thr Thr Asn Ala
905 910 915
tca ctg cgc aag ggc gat gtt cca gag tcc ctg acc gat gcg cgt gcc 2899
Ser Leu Arg Lys Gly Asp Val Pro Glu Ser Leu Thr Asp Ala Arg Ala
920 925 930
aac gag tta ctt tcc gag cgt cgt gcc aag gaa gca gca gat ggc gga 2947
Asn Glu Leu Leu Ser Glu Arg Arg Ala Lys Glu Ala Ala Asp Gly Gly
935 940 945
gct cct gcg aag aag acg tcc act aaa aag act gca gcc aag aag acc 2995
Ala Pro Ala Lys Lys Thr Ser Thr Lys Lys Thr Ala Ala Lys Lys Thr
950 955 960 965
acg gct aaa aag aca aca gct aag aaa acc gtg agg aag gct ccg ccg 3043
Thr Ala Lys Lys Thr Thr Ala Lys Lys Thr Val Arg Lys Ala Pro Pro
970 975 980
aaa acc acc aaa aac gtg gtg aag gcc ggc gct aag aag aag tcc 3088
Lys Thr Thr Lys Asn Val Val Lys Ala Gly Ala Lys Lys Lys Ser
985 990 995
taaaacatgc tgaacgggtt cgtgaccaaa 3118
<210>82
<211>996
<212>PRT
<213>谷氨酸棒状杆菌
<400>82
Val Ala Asp Thr Ala Gly Thr Thr Gly Ser Lys Lys Lys Tyr Leu Val
1 5 10 15
Ile Val Glu Ser Ala Thr Lys Ala Lys Lys Ile Gln Pro Tyr Leu Gly
20 25 30
Asn Asp Tyr Ile Val Glu Ala Ser Val Gly His Ile Arg Asp Leu Pro
35 40 45
Arg Gly Ala Ala Asp Ile Pro Ala Lys Tyr Lys Lys Glu Pro Trp Ala
50 55 60
Arg Leu Gly Val Asp Thr Asp Arg Gly Phe Ala Pro Leu Tyr Val Val
65 70 75 80
Ser Pro Asp Lys Lys Lys Lys Val Ala Asp Leu Lys Ala Lys Leu Lys
85 90 95
Leu Val Asp Glu Leu Leu Leu Ala Thr Asp Pro Asp Arg Glu Gly Glu
100 105 110
Ala Ile Ala Trp His Leu Leu Glu Val Leu Lys Pro Thr Val Pro Val
115 120 125
Arg Arg Met Val Phe Asn Glu Ile Thr Lys Pro Ala Ile Leu Ala Ala
130 135 140
Ala Glu Asn Thr Arg Glu Leu Asp Glu Asn Leu Val Asp Ala Gln Glu
145 150 155 160
Thr Arg Arg Ile Leu Asp Arg Leu Tyr Gly Tyr Glu Val Ser Pro Val
165 170 175
Leu Trp Lys Lys Val Met Pro Arg Leu Ser Ala Gly Arg Val Gln Ser
180 185 190
Val Ala Thr Arg Val Ile Val Glu Arg Glu Arg Glu Arg Met Ala Phe
195 200 205
Val Ser Ala Asp Tyr Trp Asp Leu Ser Ala Glu Phe Asn Ala Arg Glu
210 215 220
Asn Gly Lys Ala Asp Ser Asp Asn Pro Ser Ser Phe Thr Ala Arg Leu
225 230 235 240
Ser Thr Ile Asp Gly Asn Arg Val Ala Gln Gly Arg Asp Phe Asn Asp
245 250 255
Arg Gly Glu Leu Thr Ser Glu Ala Val Val Val Asp Lys Gln Arg Ala
260 265 270
Glu Ala Leu Ala Glu Ala Leu Glu Gly Gln Glu Met Ala Val Val Gly
275 280 285
Val Glu Glu Lys Pro Tyr Thr Arg Arg Pro Tyr Ala Pro Phe Met Thr
290 295 300
Ser Thr Leu Gln Gln Glu Ser Gly Arg Lys Leu His Tyr Thr Ser Glu
305 310 315 320
Arg Thr Met Arg Ile Ala Gln Arg Leu Tyr Glu Asn Gly His Ile Thr
325 330 335
Tyr Met Arg Thr Asp Ser Thr Ser Leu Ser Glu Gln Gly Met Lys Ala
340 345 350
Ala Arg Asp Gln Ala Leu Glu Leu Tyr Gly Ala Glu Tyr Val Ser Pro
355 360 365
Ser Pro Arg Thr Tyr Asp Arg Lys Val Lys Asn Ser Gln Glu Ala His
370 375 380
Glu Ala Ile Arg Pro Ala Gly Glu Thr Phe Ala Thr Pro Gly Gln Leu
385 390 395 400
His Gly Gln Leu Asp Ala Glu Glu Phe Lys Leu Tyr Glu Leu Ile Trp
405 410 415
Gln Arg Thr Val Ala Ser Gln Met Ala Asp Ala Lys Gly Thr Ser Met
420 425 430
Lys Val Thr Ile Gly Gly Thr Ala Lys Thr Gly Glu Lys Thr Glu Phe
435 440 445
Asn Ala Thr Gly Arg Thr Leu Thr Phe Pro Gly Phe Leu Arg Ala Tyr
450 455 460
Val Glu Thr Thr Arg Thr Ala Asp Gly Arg Asp Val Ala Asp Asn Ala
465 470 475 480
Glu Lys Arg Leu Pro Leu Leu Ser Glu Gly Asp Leu Leu Lys Val Leu
485 490 495
Ser Ile Glu Ala Asp Gly His Ser Thr Asn Pro Pro Ala Arg Tyr Thr
500 505 510
Glu Ala Ser Leu Val Lys Lys Met Glu Asp Leu Gly Ile Gly Arg Pro
515 520 525
Ser Thr Tyr Ala Ser Ile Ile Lys Thr Ile Gln Asp Arg Gly Tyr Val
530 535 540
Tyr Ser Arg Gly Asn Ala Leu Val Pro Ser Trp Val Ala Phe Ala Val
545 550 555 560
Val Gly Leu Leu Glu Ala Asn Phe Thr Ser Leu Val Asp Tyr Asp Phe
565 570 575
Thr Ser Ser Met Glu Asp Glu Leu Asp Asn Ile Ala Ala Gly Arg Glu
580 585 590
Gly Arg Thr Glu Trp Leu Asn Gly Phe Tyr Phe Gly Asp Ala Glu Ala
595 600 605
Asp Gln Ser Met Ala Glu Ser Val Ala Arg Gln Gly Gly Leu Lys Ala
610 615 620
Leu Val Asp Ala Asn Leu Glu His Ile Asp Ala Arg Ser Val Asn Ser
625 630 635 640
Leu Lys Leu Phe Asp Asp Ala Glu Gly Arg Ala Val Asn Val Arg Val
645 650 655
Gly Arg Tyr Gly Pro Tyr Ile Glu Arg Ile Val Gly Thr Thr Ala Glu
660 665 670
Gly Glu Pro Glu Phe Gln Arg Ala Asn Leu Pro Glu Glu Thr Thr Pro
675 680 685
Asp Glu Leu Thr Leu Glu Val Ala Glu Lys Leu Phe Ala Thr Pro Gln
690 695 700
Gly Gly Arg Glu Leu Gly Ile Asn Pro Ala Asn Gly Arg Met Val Val
705 710 715 720
Ala Lys Glu Gly Arg Phe Gly Pro Tyr Val Ile Glu Gln Val Thr Asp
725 730 735
Ser Glu Arg Ala Gly Ala Glu Ala Gln Ala Glu Glu Val Val Ala Ala
740 745 750
Glu Arg Lys Ala Glu Asp Glu Gln Arg Ala Thr Asp Gly Met Arg Pro
755 760 765
Lys Asn Trp Glu Thr Lys Thr Ala Ala Asn Gln Lys Glu Lys Arg Ile
770 775 780
Asn Gln Leu Val Glu Glu Asn Leu Lys Pro Ala Thr Ala Ser Leu Phe
785 790 795 800
Ser Gly Met Glu Pro Ala Thr Val Thr Leu Glu Glu Ala Leu Lys Leu
805 810 815
Leu Ser Leu Pro Arg Glu Val Gly Val Asp Pro Ser Asp Asn Glu Val
820 825 830
Ile Thr Ala Gln Asn Gly Arg Tyr Gly Pro Tyr Leu Lys Lys Gly Ser
835 840 845
Asp Ser Arg Ser Leu Asn Ser Glu Glu Gln Ile Phe Thr Val Thr Leu
850 855 860
Asp Glu Ala Arg Arg Ile Tyr Ala Glu Pro Lys Arg Arg Gly Arg Ala
865 870 875 880
Ala Ala Gln Pro Pro Leu Lys Gln Leu Gly Asp Asn Asp Val Ser Gly
885 890 895
Lys Pro Met Thr Val Lys Asp Gly Arg Phe Gly Pro Tyr Val Thr Asp
900 905 910
Gly Thr Thr Asn Ala Ser Leu Arg Lys Gly Asp Val Pro Glu Ser Leu
915 920 925
Thr Asp Ala Arg Ala Asn Glu Leu Leu Ser Glu Arg Arg Ala Lys Glu
930 935 940
Ala Ala Asp Gly Gly Ala Pro Ala Lys Lys Thr Ser Thr Lys Lys Thr
945 950 955 960
Ala Ala Lys Lys Thr Thr Ala Lys Lys Thr Thr Ala Lys Lys Thr Val
965 970 975
Arg Lys Ala Pro Pro Lys Thr Thr Lys Asn Val Val Lys Ala Gly Ala
980 985 990
Lys Lys Lys Ser
995
<210>83
<211>2917
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(101)..(2 887)
<223>RXA04194
<400>83
cggatgctga acccaagtgg ggaggcgtcg ataagctata aacccgggcg cgccgacttc 60
ggtgacgtgc tctagggcct agccaggtac ccttagaggc atg agt ttt tct gcc 115
Met Ser Phe Ser Ala
1 5
gaa aag ggc acc cac ctt tca gag ttc ata gca gac ctg ggc ttt gat 163
Glu Lys Gly Thr His Leu Ser Glu Phe Ile Ala Asp Leu Gly Phe Asp
10 15 20
ctg gac gag ttc caa atc aaa ggc tgc cac gct gtg gaa gaa gac cac 211
Leu Asp Glu Phe Gln Ile Lys Gly Cys His Ala Val Glu Glu Asp His
25 30 35
ggt gtt tta gta tgt gcg ccc acc ggc gcg ggc aaa aca att gtt ggt 259
Gly Val Leu Val Cys Ala Pro Thr Gly Ala Gly Lys Thr Ile Val Gly
40 45 50
gaa ttc gca gtg tcc ctc gca tta tcg cgg ggg aca aag tgt ttc tac 307
Glu Phe Ala Val Ser Leu Ala Leu Ser Arg Gly Thr Lys Cys Phe Tyr
55 60 65
acc acc ccc atc aaa gcg ctg agc aac cag aag tac cac gat ttg gtg 355
Thr Thr Pro Ile Lys Ala Leu Ser Asn Gln Lys Tyr His Asp Leu Val
70 75 80 85
gct aaa cac ggc tcc gat gcc gtt ggt ctg ctc acc ggt gat gtt tcc 403
Ala Lys His Gly Ser Asp Ala Val Gly Leu Leu Thr Gly Asp Val Ser
90 95 100
att aac cat gat gct gac atc gtg gtc atg acc acc gaa gtg ctg cgc 451
Ile Asn His Asp Ala Asp Ile Val Val Met Thr Thr Glu Val Leu Arg
105 110 115
aac atg att tac gcg ggc tct ttt gcg ctt gag cgc tta agc cac gtg 499
Asn Met Ile Tyr Ala Gly Ser Phe Ala Leu Glu Arg Leu Ser His Val
120 125 130
gtc atg gat gag atc cac ttc ctt gct gat gcc tcc cgt ggc gcg gtg 547
Val Met Asp Glu Ile His Phe Leu Ala Asp Ala Ser Arg Gly Ala Val
135 140 145
tgg gaa gaa gtg atc ctc aac ttg gat gat tcc gtc aac atc atc ggt 595
Trp Glu Glu Val Ile Leu Asn Leu Asp Asp Ser Val Asn Ile Ile Gly
150 155 160 165
cta tct gcc acg gtg tcc aac tca gag gag ttt ggt gag tgg ctg acc 643
Leu Ser Ala Thr Val Ser Asn Ser Glu Glu Phe Gly Glu Trp Leu Thr
170 175 180
act gtt cgc ggc gat acc cgt gtg att gtt act gat cac cgc ccc gtt 691
Thr Val Arg Gly Asp Thr Arg Val Ile Val Thr Asp His Arg Pro Val
185 190 195
ccg ctt gat cag tac atg atg gtg cag cgc aaa gtg atg cca ctg ttt 739
Pro Leu Asp Gln Tyr Met Met Val Gln Arg Lys Val Met Pro Leu Phe
200 205 210
gag cct ggc acc gat gga cgc gtg aac aag gag tta gag gca acg att 787
Glu Pro Gly Thr Asp Gly Arg Val Asn Lys Glu Leu Glu Ala Thr Ile
215 220 225
gat cgc ctc aac agc aag caa agc gaa caa ggc cgt gcg gca tac cgc 835
Asp Arg Leu Asn Ser Lys Gln Ser Glu Gln Gly Arg Ala Ala Tyr Arg
230 235 240 245
tct ggt gaa ggc ttc cgt gca cgc agc aaa ggc gat aag cag gat tct 883
Ser Gly Glu Gly Phe Arg Ala Arg Ser Lys Gly Asp Lys Gln Asp Ser
250 255 260
cgc act ggt aag cca cgg gaa caa gac cgc cac agg cca ctg ggt cgg 931
Arg Thr Gly Lys Pro Arg Glu Gln Asp Arg His Arg Pro Leu Gly Arg
265 270 275
cct gaa gtg ctc agc atc ctc aag ggc atc aac atg ctg cca gcg att 979
Pro Glu Val Leu Ser Ile Leu Lys Gly Ile Asn Met Leu Pro Ala Ile
280 285 290
acg ttt atc ttc tcc cgc gcg ggc tgt gat ggt gcg ctg tac caa tgc 1027
Thr Phe Ile Phe Ser Arg Ala Gly Cys Asp Gly Ala Leu Tyr Gln Cys
295 300 305
ttg cgt tct aag ttg gtc ttg acg gat caa gca gaa tca gaa gag att 1075
Leu Arg Ser Lys Leu Val Leu Thr Asp Gln Ala Glu Ser Glu Glu Ile
310 315 320 325
gca cgc att gtc gac gcc ggc gtg gtg ggg atc ccc gag gaa gac ctt 1123
Ala Arg Ile Val Asp Ala Gly Val Val Gly Ile Pro Glu Glu Asp Leu
330 335 340
caa gta ctg aac ttt aag cag tgg cgt gct gca ctg atg cgc ggt ttc 1171
Gln Val Leu Asn Phe Lys Gln Trp Arg Ala Ala Leu Met Arg Gly Phe
345 350 355
gca gcc cac cac gcg ggt atg ctt cca gcg ttt agg cac atc gtg gaa 1219
Ala Ala His His Ala Gly Met Leu Pro Ala Phe Arg His Ile Val Glu
360 365 370
gag ctc ttt gtt aaa ggt ctt gtc cgc gcg gtg ttt gcc acg gaa acc 1267
Glu Leu Phe Val Lys Gly Leu Val Arg Ala Val Phe Ala Thr Glu Thr
375 380 385
ctg gca ttg gga atc aac atg cca gcg cgc acc gtg gtg ttg gaa aag 1315
Leu Ala Leu Gly Ile Asn Met Pro Ala Arg Thr Val Val Leu Glu Lys
390 395 400 405
atg gtc aaa ttt gac ggc gaa ggc cac gtt gat ctc acc cct ggc caa 1363
Met Val Lys Phe Asp Gly Glu Gly His Val Asp Leu Thr Pro Gly Gln
410 415 420
tac acg cag ctg acc ggt cgt gct ggt cga cgt ggc atc gat gtg ttg 1411
Tyr Thr Gln Leu Thr Gly Arg Ala Gly Arg Arg Gly Ile Asp Val Leu
425 430 435
ggt aat gct gtg gtg cag tgg tca cca gca ctt gat cca cga tgg gtg 1459
Gly Asn Ala Val Val Gln Trp Ser Pro Ala Leu Asp Pro Arg Trp Val
440 445 450
gca ggt ctt gcc tct acg cgt acc tac ccg ctg atc tct acg ttc cag 1507
Ala Gly Leu Ala Ser Thr Arg Thr Tyr Pro Leu Ile Ser Thr Phe Gln
455 460 465
ccg ggc tac aac atg tcg gtt aac ctg ctg aaa acc att ggt tat gag 1555
Pro Gly Tyr Asn Met Ser Val Asn Leu Leu Lys Thr Ile Gly Tyr Glu
470 475 480 485
cct tcg ctg cgc ctt ttg gaa aaa tct ttt gca cag ttc caa gcc gat 1603
Pro Ser Leu Arg Leu Leu Glu Lys Ser Phe Ala Gln Phe Gln Ala Asp
490 495 500
ggt tcc gtc gtg ggc gat gtg cgt gaa att gaa cgt gca gaa gcc aag 1651
Gly Ser Val Val Gly Asp Val Arg Glu Ile Glu Arg Ala Glu Ala Lys
505 510 515
gtg gca gaa ttg cgt gcc cag ctg aac aaa gag att gct gcc acc aac 1699
Val Ala Glu Leu Arg Ala Gln Leu Asn Lys Glu Ile Ala Ala Thr Asn
520 525 530
cct gcg gtg cgt gaa gat caa gac gca gtg gag gtg ttt atc gac tac 1747
Pro Ala Val Arg Glu Asp Gln Asp Ala Val Glu Val Phe Ile Asp Tyr
535 540 545
atg gag ctt cgc cgt gag ctc aat gag gaa gaa aaa ctc aat cgc aag 1795
Met Glu Leu Arg Arg Glu Leu Asn Glu Glu Glu Lys Leu Asn Arg Lys
550 555 560 565
caa agc atc gaa gat cgc aac gta gaa aca gtc cgt gtg ctg tcc aag 1843
Gln Ser Ile Glu Asp Arg Asn Val Glu Thr Val Arg Val Leu Ser Lys
570 575 580
ctg caa atc ggt gat gtc att gcc atg cct ggt aga aaa cga cca gtt 1891
Leu Gln Ile Gly Asp Val Ile Ala Met Pro Gly Arg Lys Arg Pro Val
585 590 595
ctc gca gta gtg atg acg cct gct aat cag agc aga gat ccc cgc cca 1939
Leu Ala Val Val Met Thr Pro Ala Asn Gln Ser Arg Asp Pro Arg Pro
600 605 610
tgg gtg acc acc gaa tct ggt tgg tca ggt cgt gtg gat gcg gaa tcc 1987
Trp Val Thr Thr Glu Ser Gly Trp Ser Gly Arg Val Asp Ala Glu Ser
615 620 625
ttt act aat ccg ccg atc acc att ggg cat atg cgt ctg cct cgc caa 2035
Phe Thr Asn Pro Pro Ile Thr Ile Gly His Met Arg Leu Pro Arg Gln
630 635 640 645
gct att gaa gag cct cgc cgc aat gcc cgg cgc gtc cag gaa ctg ttt 2083
Ala Ile Glu Glu Pro Arg Arg Asn Ala Arg Arg Val Gln Glu Leu Phe
650 655 660
agg cgt gag cac ttc aag cga ccc aac aag atg cgg gag ttt gct cgg 2131
Arg Arg Glu His Phe Lys Arg Pro Asn Lys Met Arg Glu Phe Ala Arg
665 670 675
gtt cgt ccc aat gag gca gtg acc aaa ctg cgt aat gct att cgg gat 2179
Val Arg Pro Asn Glu Ala Val Thr Lys Leu Arg Asn Ala Ile Arg Asp
680 685 690
cat gag gcg cat cat tgg cct gat cgg gag cac ttg gct cgc acc gcg 2227
His Glu Ala His His Trp Pro Asp Arg Glu His Leu Ala Arg Thr Ala
695 700 705
gag cgt atg atc cgt aaa gaa cgt gat ctg gct aag ttg acc ggc aat 2275
Glu Arg Met Ile Arg Lys Glu Arg Asp Leu Ala Lys Leu Thr Gly Asn
710 715 720 725
gtg gat aaa gcc agg gaa acc ctc ggt agg acg ttt gag cgc att ttg 2323
Val Asp Lys Ala Arg Glu Thr Leu Gly Arg Thr Phe Glu Arg Ile Leu
730 735 740
tcg ctg ctc agt gaa atg gac tat gtg gat tac tct aat cca gat aat 2371
Ser Leu Leu Ser Glu Met Asp Tyr Val Asp Tyr Ser Asn Pro Asp Asn
745 750 755
cca gtg atc act gat gaa ggt gag cgt ttg gcg aaa atc cac agt gag 2419
Pro Val Ile Thr Asp Glu Gly Glu Arg Leu Ala Lys Ile His Ser Glu
760 765 770
gca gac ctg ttg gtt gct cag tgc ctc aag cgt ggc att tgg gac aac 2467
Ala Asp Leu Leu Val Ala Gln Cys Leu Lys Arg Gly Ile Trp Asp Asn
775 780 785
ctt gat ccc gca gag ctc gcc ggt gtg gtg agt atg tgc acg ttt gaa 2515
Leu Asp Pro Ala Glu Leu Ala Gly Val Val Ser Met Cys Thr Phe Glu
790 795 800 805
aat cgt cgc gaa acc ggt ggt gag gct caa gct gtc aca gag gcc atg 2563
Asn Arg Arg Glu Thr Gly Gly Glu Ala Gln Ala Val Thr Glu Ala Met
810 815 820
gct gat tcc atg aat agc gtg gaa cgt att tgg ggt gag ctg gtt gaa 2611
Ala Asp Ser Met Asn Ser Val Glu Arg Ile Trp Gly Glu Leu Val Glu
825 830 835
gat gag cgc cgt cac cgt ctg cca att act cgc cag ccg gaa gca ggt 2559
Asp Glu Arg Arg His Arg Leu Pro Ile Thr Arg Gln Pro Glu Ala Gly
840 845 850
ttt gcc acc gcg atc cac cag tgg gct tca ggt gct ccg ctg gga tat 2707
Phe Ala Thr Ala Ile His Gln Trp Ala Ser Gly Ala Pro Leu Gly Tyr
855 860 865
tgc atg gct gcg gca gcg gaa aac ggc gcg gag ttg acc cct ggt gac 2755
Cys Met Ala Ala Ala Ala Glu Asn Gly Ala Glu Leu Thr Pro Gly Asp
870 875 880 885
ttc gtg cgc tgg tgc cgt caa gtc atc gat ctt cta gag cag gtt gct 2803
Phe Val Arg Trp Cys Arg Gln Val Ile Asp Leu Leu Glu Gln Val Ala
890 895 900
aag act gcc tac ttt gat gag acc aca cgg aat gct cgt cag gct att 2851
Lys Thr Ala Tyr Phe Asp Glu Thr Thr Arg Asn Ala Arg Gln Ala Ile
905 910 915
gat gcg att agg cgt gga gtt gtg gcg atc ggt tcc tagcgagcta 2897
Asp Ala Ile Arg Arg Gly Val Val Ala Ile Gly Ser
920 925
gctatgttcg agcagttcaa 2917
<210>84
<211>929
<212>PRT
<213>谷氨酸棒状杆菌
<400>84
Met Ser Phe Ser Ala Glu Lys Gly Thr His Leu Ser Glu Phe Ile Ala
1 5 10 15
Asp Leu Gly Phe Asp Leu Asp Glu Phe Gln Ile Lys Gly Cys His Ala
20 25 30
Val Glu Glu Asp His Gly Val Leu Val Cys Ala Pro Thr Gly Ala Gly
35 40 45
Lys Thr Ile Val Gly Glu Phe Ala Val Ser Leu Ala Leu Ser Arg Gly
50 55 60
Thr Lys Cys Phe Tyr Thr Thr Pro Ile Lys Ala Leu Ser Asn Gln Lys
65 70 75 80
Tyr His Asp Leu Val Ala Lys His Gly Ser Asp Ala Val Gly Leu Leu
85 90 95
Thr Gly Asp Val Ser Ile Asn His Asp Ala Asp Ile Val Val Met Thr
100 105 110
Thr Glu Val Leu Arg Asn Met Ile Tyr Ala Gly Ser Phe Ala Leu Glu
115 120 125
Arg Leu Ser His Val Val Met Asp Glu Ile His Phe Leu Ala Asp Ala
130 135 140
Ser Arg Gly Ala Val Trp Glu Glu Val Ile Leu Asn Leu Asp Asp Ser
145 150 155 160
Val Asn Ile Ile Gly Leu Ser Ala Thr Val Ser Asn Ser Glu Glu Phe
165 170 175
Gly Glu Trp Leu Thr Thr Val Arg Gly Asp Thr Arg Val Ile Val Thr
180 185 190
Asp His Arg Pro Val Pro Leu Asp Gln Tyr Met Met Val Gln Arg Lys
195 200 205
Val Met Pro Leu Phe Glu Pro Gly Thr Asp Gly Arg Val Asn Lys Glu
210 215 220
Leu Glu Ala Thr Ile Asp Arg Leu Asn Ser Lys Gln Ser Glu Gln Gly
225 230 235 240
Arg Ala Ala Tyr Arg Ser Gly Glu Gly Phe Arg Ala Arg Ser Lys Gly
245 250 255
Asp Lys Gln Asp Ser Arg Thr Gly Lys Pro Arg Glu Gln Asp Arg His
260 265 270
Arg Pro Leu Gly Arg Pro Glu Val Leu Ser Ile Leu Lys Gly Ile Asn
275 280 285
Met Leu Pro Ala Ile Thr Phe Ile Phe Ser Arg Ala Gly Cys Asp Gly
290 295 300
Ala Leu Tyr Gln Cys Leu Arg Ser Lys Leu Val Leu Thr Asp Gln Ala
305 310 315 320
Glu Ser Glu Glu Ile Ala Arg Ile Val Asp Ala Gly Val Val Gly Ile
325 330 335
Pro Glu Glu Asp Leu Gln Val Leu Asn Phe Lys Gln Trp Arg Ala Ala
340 345 350
Leu Met Arg Gly Phe Ala Ala His His Ala Gly Met Leu Pro Ala Phe
355 360 365
Arg His Ile Val Glu Glu Leu Phe Val Lys Gly Leu Val Arg Ala Val
370 375 380
Phe Ala Thr Glu Thr Leu Ala Leu Gly Ile Asn Met Pro Ala Arg Thr
385 390 395 400
Val Val Leu Glu Lys Met Val Lys Phe Asp Gly Glu Gly His Val Asp
405 410 415
Leu Thr Pro Gly Gln Tyr Thr Gln Leu Thr Gly Arg Ala Gly Arg Arg
420 425 430
Gly Ile Asp Val Leu Gly Asn Ala Val Val Gln Trp Ser Pro Ala Leu
435 440 445
Asp Pro Arg Trp Val Ala Gly Leu Ala Ser Thr Arg Thr Tyr Pro Leu
450 455 460
Ile Ser Thr Phe Gln Pro Gly Tyr Asn Met Ser Val Asn Leu Leu Lys
465 470 475 480
Thr Ile Gly Tyr Glu Pro Ser Leu Arg Leu Leu Glu Lys Ser Phe Ala
485 490 495
Gln Phe Gln Ala Asp Gly Ser Val Val Gly Asp Val Arg Glu Ile Glu
500 505 510
Arg Ala Glu Ala Lys Val Ala Glu Leu Arg Ala Gln Leu Asn Lys Glu
515 520 525
Ile Ala Ala Thr Asn Pro Ala Val Arg Glu Asp Gln Asp Ala Val Glu
530 535 540
Val Phe Ile Asp Tyr Met Glu Leu Arg Arg Glu Leu Asn Glu Glu Glu
545 550 555 560
Lys Leu Asn Arg Lys Gln Ser Ile Glu Asp Arg Asn Val Glu Thr Val
565 570 575
Arg Val Leu Ser Lys Leu Gln Ile Gly Asp Val Ile Ala Met Pro Gly
580 585 590
Arg Lys Arg Pro Val Leu Ala Val Val Met Thr Pro Ala Asn Gln Ser
595 600 605
Arg Asp Pro Arg Pro Trp Val Thr Thr Glu Ser Gly Trp Ser Gly Arg
610 615 620
Val Asp Ala Glu Ser Phe Thr Asn Pro Pro Ile Thr Ile Gly His Met
625 630 635 640
Arg Leu Pro Arg Gln Ala Ile Glu Glu Pro Arg Arg Asn Ala Arg Arg
645 650 655
Val Gln Glu Leu Phe Arg Arg Glu His Phe Lys Arg Pro Asn Lys Met
660 665 670
Arg Glu Phe Ala Arg Val Arg Pro Asn Glu Ala Val Thr Lys Leu Arg
675 680 685
Asn Ala Ile Arg Asp His Glu Ala His His Trp Pro Asp Arg Glu His
690 695 700
Leu Ala Arg Thr Ala Glu Arg Met Ile Arg Lys Glu Arg Asp Leu Ala
705 710 715 720
Lys Leu Thr Gly Asn Val Asp Lys Ala Arg Glu Thr Leu Gly Arg Thr
725 730 735
Phe Glu Arg Ile Leu Ser Leu Leu Ser Glu Met Asp Tyr Val Asp Tyr
740 745 750
Ser Asn Pro Asp Asn Pro Val Ile Thr Asp Glu Gly Glu Arg Leu Ala
755 760 765
Lys Ile His Ser Glu Ala Asp Leu Leu Val Ala Gln Cys Leu Lys Arg
770 775 780
Gly Ile Trp Asp Asn Leu Asp Pro Ala Glu Leu Ala Gly Val Val Ser
785 790 795 800
Met Cys Thr Phe Glu Asn Arg Arg Glu Thr Gly Gly Glu Ala Gln Ala
805 810 815
Val Thr Glu Ala Met Ala Asp Ser Met Asn Ser Val Glu Arg Ile Trp
820 825 830
Gly Glu Leu Val Glu Asp Glu Arg Arg His Arg Leu Pro Ile Thr Arg
835 840 845
Gln Pro Glu Ala Gly Phe Ala Thr Ala Ile His Gln Trp Ala Ser Gly
850 855 860
Ala Pro Leu Gly Tyr Cys Met Ala Ala Ala Ala Glu Asn Gly Ala Glu
865 870 875 880
Leu Thr Pro Gly Asp Phe Val Arg Trp Cys Arg Gln Val Ile Asp Leu
885 890 895
Leu Glu Gln Val Ala Lys Thr Ala Tyr Phe Asp Glu Thr Thr Arg Asn
900 905 910
Ala Arg Gln Ala Ile Asp Ala Ile Arg Arg Gly Val Val Ala Ile Gly
915 920 925
Ser
<210>85
<211>2325
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(2325)
<223>RXA07017
<400>85
gtg gct ttg tat agc aag tat cga ccg gca agt ttt ggt gaa cta gtt 48
Val Ala Leu Tyr Ser Lys Tyr Arg Pro Ala Ser Phe Gly Glu Leu Val
1 5 10 15
ggg cag tcg caa gtg act gac cct ctg tcc gca gct ttg gat agc ggg 96
Gly Gln Ser Gln Val Thr Asp Pro Leu Ser Ala Ala Leu Asp Ser Gly
20 25 30
cgc atc aac cat gcg tac ctt ttt tcg ggt ccg cgt ggt tgt ggc aag 144
Arg Ile Asn His Ala Tyr Leu Phe Ser Gly Pro Arg Gly Cys Gly Lys
35 40 45
acg tcg tcg gca cgc atc ctt gcc cgg tcc ctc aac tgc gtg gaa ggc 192
Thr Ser Ser Ala Arg Ile Leu Ala Arg Ser Leu Asn Cys Val Glu Gly
50 55 60
cca act tcc acg ccg tgt ggg gtg tgt aat tct tgc gta gcg ctg gcc 240
Pro Thr Ser Thr Pro Cys Gly Val Cys Asn Ser Cys Val Ala Leu Ala
65 70 75 80
ccg ggt ggc cct gga acc ctt gat gta aca gag ctc gac gcc gcg agt 288
Pro Gly Gly Pro Gly Thr Leu Asp Val Thr Glu Leu Asp Ala Ala Ser
85 90 95
aac aat ggt gtc gat gac atg cgt gaa ctg cgc gaa cgc gcg aac tac 336
Asn Asn Gly Val Asp Asp Met Arg Glu Leu Arg Glu Arg Ala Asn Tyr
100 105 110
gcc ccg gcg gaa tct cgc tac cgc gtg ttc atc att gac gaa gcc cac 384
Ala Pro Ala Glu Ser Arg Tyr Arg Val Phe Ile Ile Asp Glu Ala His
115 120 125
atg atc agt acc caa ggc ttc aac gcc ttg ctg aaa atc gtt gaa gag 432
Met Ile Ser Thr Gln Gly Phe Asn Ala Leu Leu Lys Ile Val Glu Glu
130 135 140
cca cca gcg cac ctg att ttc atc ttc gcc acc acc gag ccg gac aaa 480
Pro Pro Ala His Leu Ile Phe Ile Phe Ala Thr Thr Glu Pro Asp Lys
145 150 155 160
atg atc ggt acg atc cgt tcc cgc acg cac aat tac cca ttc cgc ctg 528
Met Ile Gly Thr Ile Arg Ser Arg Thr His Asn Tyr Pro Phe Arg Leu
165 170 175
ctc acc cca ggg gat atg cgc aaa gtg ctg aaa aat gcg gtc gat ggc 576
Leu Thr Pro Gly Asp Met Arg Lys Val Leu Lys Asn Ala Val Asp Gly
180 185 190
gaa ggc gtc cac gtc gac gat tcc gtt tac cca ctg gtc atc cgc gcc 624
Glu Gly Val His Val Asp Asp Ser Val Tyr Pro Leu Val Ile Arg Ala
195 200 205
ggc ggc ggc agc ccc cgc gac agc ctc tcc atc ctc gac cag ctc atc 672
Gly Gly Gly Ser Pro Arg Asp Ser Leu Ser Ile Leu Asp Gln Leu Ile
210 215 220
gcc ggc tcg ggc ccg gag ggc ttg aca tat gag cgc gcc ttg ccg ctg 720
Ala Gly Ser Gly Pro Glu Gly Leu Thr Tyr Glu Arg Ala Leu Pro Leu
225 230 235 240
ctc ggt gtc aca agc ttc acg ctt atc gac gat tcg atc cat gcc ctt 768
Leu Gly Val Thr Ser Phe Thr Leu Ile Asp Asp Ser Ile His Ala Leu
245 250 255
gca tct aaa gac aac gca agc atg ttc acc acg atc gat aac gtc atc 8l6
Ala Ser Lys Asp Asn Ala Ser Met Phe Thr Thr Ile Asp Asn Val Ile
260 265 270
gaa gaa ggc ctc gaa ccg cga cgc ttc acg atc gac ctc ctc gac cgc 864
Glu Glu Gly Leu Glu Pro Arg Arg Phe Thr Ile Asp Leu Leu Asp Arg
275 280 285
ctc cgc gac ttg atg gtc ctc caa gcc gtc cca gaa gca cta aac ctc 912
Leu Arg Asp Leu Met Val Leu Gln Ala Val Pro Glu Ala Leu Asn Leu
290 295 300
ggg ctt gtc gac gcc ccc acc gac cgc gcc gac gcc cta atc gaa caa 960
Gly Leu Val Asp Ala Pro Thr Asp Arg Ala Asp Ala Leu Ile Glu Gln
305 310 315 320
tcc acc ctg ttc aaa ggc aac gag ctc gcc aac cta gcc tcc atg gtg 1008
Ser Thr Leu Phe Lys Gly Asn Glu Leu Ala Asn Leu Ala Ser Met Val
325 330 335
aac tcc gga ctc gac gac atg cgt ggt gcc acc tca ccg cgc ctc ctt 1056
Asn Ser Gly Leu Asp Asp Met Arg Gly Ala Thr Ser Pro Arg Leu Leu
340 345 350
ctg gaa atc ctc tgc gcc cga ctg ctc ctg gca agc aat acc gtg gca 1104
Leu Glu Ile Leu Cys Ala Arg Leu Leu Leu Ala Ser Asn Thr Val Ala
355 360 365
ggt cca gcg gtc agt agt tcg act gac gct gcg cct gca gct act ccg 1152
Gly Pro Ala Val Ser Ser Ser Thr Asp Ala Ala Pro Ala Ala Thr Pro
370 375 380
ggt ggt ctc act ggt att gct gct gcc cgc gcg aaa gca cgg gag tat 1200
Gly Gly Leu Thr Gly Ile Ala Ala Ala Arg Ala Lys Ala Arg Glu Tyr
385 390 395 400
gga cag aag aag gca gct cca gct cct gca cca act cct gcg ccc gag 1248
Gly Gln Lys Lys Ala Ala Pro Ala Pro Ala Pro Thr Pro Ala Pro Glu
405 410 415
cca gtg cgc gaa cag tct ctt gca cca acg cct gaa cca acg cca gcg 1296
Pro Val Arg Glu Gln Ser Leu Ala Pro Thr Pro Glu Pro Thr Pro Ala
420 425 430
gct gaa cct aca tct cag ccc gcg ccg gaa ccg gaa ccc gcc agg gaa 1344
Ala Glu Pro Thr Ser Gln Pro Ala Pro Glu Pro Glu Pro Ala Arg Glu
435 440 445
cca gtg gtg gaa gtg cgg gag gcg agc gtc gaa aag cag cct gca agc 1392
Pro Val Val Glu Val Arg Glu Ala Ser Val Glu Lys Gln Pro Ala Ser
450 455 460
agt gat ccc ctc gaa acc att cga agc cgc tgg tca gag ctg cgc aac 1440
Ser Asp Pro Leu Glu Thr Ile Arg Ser Arg Trp Ser Glu Leu Arg Asn
465 470 475 480
atc gtg gaa aaa caa agt gtg cgc acc tca atc atg ctg acc gaa gcg 1488
Ile Val Glu Lys Gln Ser Val Arg Thr Ser Ile Met Leu Thr Glu Ala
485 490 495
cga gtt ttg gga ctg cga ggc gat acc ctc gtg ctc ggt cac agc acc 1536
Arg Val Leu Gly Leu Arg Gly Asp Thr Leu Val Leu Gly His Ser Thr
500 505 510
ggg gcg ttg gct gcg cgt ttg aac gct gct gat cac aac gga att ttg 1584
Gly Ala Leu Ala Ala Arg Leu Asn Ala Ala Asp His Asn Gly Ile Leu
515 520 525
gtc aag gtg ttg gct gag gaa act ggt ctg cag ctc aag gtc gaa tgc 1632
Val Lys Val Leu Ala Glu Glu Thr Gly Leu Gln Leu Lys Val Glu Cys
530 535 540
att gtg ggc acg aac cca gcc gaa gct gga ttt acc gcc cag cct gct 1680
Ile Val Gly Thr Asn Pro Ala Glu Ala Gly Phe Thr Ala Gln Pro Ala
545 550 555 560
gtg caa aag agc acg tgg aac ccc aac tac gac tcc aaa cca gcc act 1728
Val Gln Lys Ser Thr Trp Asn Pro Asn Tyr Asp Ser Lys Pro Ala Thr
565 570 575
cca agt gcg cct gcg cag cct cag acg cct aag caa gaa tcc gtg ccc 1776
Pro Ser Ala Pro Ala Gln Pro Gln Thr Pro Lys Gln Glu Ser Val Pro
580 585 590
acc gag cca gag gag cct gag agt tcg gct gaa act tcg gga tgg ggg 1824
Thr Glu Pro Glu Glu Pro Glu Ser Ser Ala Glu Thr Ser Gly Trp Gly
595 600 605
caa ccg gta aaa atc ggt ggg cca gca cct gag gca cag act cca acg 1872
Gln Pro Val Lys Ile Gly Gly Pro Ala Pro Glu Ala Gln Thr Pro Thr
610 615 620
cct ccg cct gct ccg gtg gtt ccg gct act cct gct gct ccg gct gcg 1920
Pro Pro Pro Ala Pro Val Val Pro Ala Thr Pro Ala Ala Pro Ala Ala
625 630 635 640
ggt gct gcg aaa cca gcg tgg aag gaa cgc gtc gaa caa gcc gcg gca 1968
Gly Ala Ala Lys Pro Ala Trp Lys Glu Arg Val Glu Gln Ala Ala Ala
645 650 655
aat gca gcg caa caa cgt cag cat cgc cag ggc agc tcc gag ccc ttc 2016
Asn Ala Ala Gln Gln Arg Gln His Arg Gln Gly Ser Ser Glu Pro Phe
660 665 670
gag cgg ggt gtt ccg ctg cca cca gaa cct gac ctg cca ccg gac ccc 2064
Glu Arg Gly Val Pro Leu Pro Pro Glu Pro Asp Leu Pro Pro Asp Pro
675 680 685
tat gga tac cca gct gac gag ggt ttc ccc gaa cga aac caa ggg ttc 2112
Tyr Gly Tyr Pro Ala Asp Glu Gly Phe Pro Glu Arg Asn Gln Gly Phe
690 695 700
cag caa cag cca gcg cct ccc gtt gag caa cca gct cct gag cct gcg 2160
Gln Gln Gln Pro Ala Pro Pro Val Glu Gln Pro Ala Pro Glu Pro Ala
705 710 715 720
acg gag cca gtt cca gca gtt gcg cct gag ccg gag gtc agc cag cta 2208
Thr Glu Pro Val Pro Ala Val Ala Pro Glu Pro Glu Val Ser Gln Leu
725 730 735
tct gaa gaa gag cag ctg atc cgc gaa gca gac gaa gag cca ggt gaa 2256
Ser Glu Glu Glu Gln Leu Ile Arg Glu Ala Asp Glu Glu Pro Gly Glu
740 745 750
atg gat cgt cgt gat gcg aaa acc atc gcg atg gaa ctg ctt gct cag 2304
Met Asp Arg Arg Asp Ala Lys Thr Ile Ala Met Glu Leu Leu Ala Gln
755 760 765
gaa ctc ggc gcg aaa cct ttg 2325
Glu Leu Gly Ala Lys Pro Leu
770 775
<210>86
<211>775
<212>PRT
<213>谷氨酸棒状杆菌
<400>86
Val Ala Leu Tyr Ser Lys Tyr Arg Pro Ala Ser Phe Gly Glu Leu Val
1 5 10 15
Gly Gln Ser Gln Val Thr Asp Pro Leu Ser Ala Ala Leu Asp Ser Gly
20 25 30
Arg Ile Asn His Ala Tyr Leu Phe Ser Gly Pro Arg Gly Cys Gly Lys
35 40 45
Thr Ser Ser Ala Arg Ile Leu Ala Arg Ser Leu Asn Cys Val Glu Gly
50 55 60
Pro Thr Ser Thr Pro Cys Gly Val Cys Asn Ser Cys Val Ala Leu Ala
65 70 75 80
Pro Gly Gly Pro Gly Thr Leu Asp Val Thr Glu Leu Asp Ala Ala Ser
85 90 95
Asn Asn Gly Val Asp Asp Met Arg Glu Leu Arg Glu Arg Ala Asn Tyr
100 105 110
Ala Pro Ala Glu Ser Arg Tyr Arg Val Phe Ile Ile Asp Glu Ala His
115 120 125
Met Ile Ser Thr Gln Gly Phe Asn Ala Leu Leu Lys Ile Val Glu Glu
130 135 140
Pro Pro Ala His Leu Ile Phe Ile Phe Ala Thr Thr Glu Pro Asp Lys
145 150 155 160
Met Ile Gly Thr Ile Arg Ser Arg Thr His Asn Tyr Pro Phe Arg Leu
165 170 175
Leu Thr Pro Gly Asp Met Arg Lys Val Leu Lys Asn Ala Val Asp Gly
180 185 190
Glu Gly Val His Val Asp Asp Ser Val Tyr Pro Leu Val Ile Arg Ala
195 200 205
Gly Gly Gly Ser Pro Arg Asp Ser Leu Ser Ile Leu Asp Gln Leu Ile
210 215 220
Ala Gly Ser Gly Pro Glu Gly Leu Thr Tyr Glu Arg Ala Leu Pro Leu
225 230 235 240
Leu Gly Val Thr Ser Phe Thr Leu Ile Asp Asp Ser Ile His Ala Leu
245 250 255
Ala Ser Lys Asp Asn Ala Ser Met Phe Thr Thr Ile Asp Asn Val Ile
260 265 270
Glu Glu Gly Leu Glu Pro Arg Arg Phe Thr Ile Asp Leu Leu Asp Arg
275 280 285
Leu Arg Asp Leu Met Val Leu Gln Ala Val Pro Glu Ala Leu Asn Leu
290 295 300
Gly Leu Val Asp Ala Pro Thr Asp Arg Ala Asp Ala Leu Ile Glu Gln
305 310 315 320
Ser Thr Leu Phe Lys Gly Asn Glu Leu Ala Asn Leu Ala Ser Met Val
325 330 335
Asn Ser Gly Leu Asp Asp Met Arg Gly Ala Thr Ser Pro Arg Leu Leu
340 345 350
Leu Glu Ile Leu Cys Ala Arg Leu Leu Leu Ala Ser Asn Thr Val Ala
355 360 365
Gly Pro Ala Val Ser Ser Ser Thr Asp Ala Ala Pro Ala Ala Thr Pro
370 375 380
Gly Gly Leu Thr Gly Ile Ala Ala Ala Arg Ala Lys Ala Arg Glu Tyr
385 390 395 400
Gly Gln Lys Lys Ala Ala Pro Ala Pro Ala Pro Thr Pro Ala Pro Glu
405 410 415
Pro Val Arg Glu Gln Ser Leu Ala Pro Thr Pro Glu Pro Thr Pro Ala
420 425 430
Ala Glu Pro Thr Ser Gln Pro Ala Pro Glu Pro Glu Pro Ala Arg Glu
435 440 445
Pro Val Val Glu Val Arg Glu Ala Ser Val Glu Lys Gln Pro Ala Ser
450 455 460
Ser Asp Pro Leu Glu Thr Ile Arg Ser Arg Trp Ser Glu Leu Arg Asn
465 470 475 480
Ile Val Glu Lys Gln Ser Val Arg Thr Ser Ile Met Leu Thr Glu Ala
485 490 495
Arg Val Leu Gly Leu Arg Gly Asp Thr Leu Val Leu Gly His Ser Thr
500 505 510
Gly Ala Leu Ala Ala Arg Leu Asn Ala Ala Asp His Asn Gly Ile Leu
515 520 525
Val Lys Val Leu Ala Glu Glu Thr Gly Leu Gln Leu Lys Val Glu Cys
530 535 540
Ile Val Gly Thr Asn Pro Ala Glu Ala Gly Phe Thr Ala Gln Pro Ala
545 550 555 560
Val Gln Lys Ser Thr Trp Asn Pro Asn Tyr Asp Ser Lys Pro Ala Thr
565 570 575
Pro Ser Ala Pro Ala Gln Pro Gln Thr Pro Lys Gln Glu Ser Val Pro
580 585 590
Thr Glu Pro Glu Glu Pro Glu Ser Ser Ala Glu Thr Ser Gly TrP Gly
595 600 605
Gln Pro Val Lys Ile Gly Gly Pro Ala Pro Glu Ala Gln Thr Pro Thr
610 615 620
Pro Pro Pro Ala Pro Val Val Pro Ala Thr Pro Ala Ala Pro Ala Ala
625 630 635 640
Gly Ala Ala Lys Pro Ala Trp Lys Glu Arg Val Glu Gln Ala Ala Ala
645 650 655
Asn Ala Ala Gln Gln Arg Gln His Arg Gln Gly Ser Ser Glu Pro Phe
660 665 670
Glu Arg Gly Val Pro Leu Pro Pro Glu Pro Asp Leu Pro Pro Asp Pro
675 680 685
Tyr Gly Tyr Pro Ala Asp Glu Gly Phe Pro Glu Arg Asn Gln Gly Phe
690 695 700
Gln Gln Gln Pro Ala Pro Pro Val Glu Gln Pro Ala Pro Glu Pro Ala
705 710 715 720
Thr Glu Pro Val Pro Ala Val Ala Pro Glu Pro Glu Val Ser Gln Leu
725 730 735
Ser Glu Glu Glu Gln Leu Ile Arg Glu Ala Asp Glu Glu Pro Gly Glu
740 745 750
Met Asp Arg Arg Asp Ala Lys Thr Ile Ala Met Glu Leu Leu Ala Gln
755 760 765
Glu Leu Gly Ala Lys Pro Leu
770 775
<210>87
<211>4026
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(4026)
<223>RXA07018
<400>87
atg ttc tgg ggg gaa agg gag ttt tac gtg ctc gac gta aac gtc ttc 48
Met Phe Trp Gly Glu Arg Glu Phe Tyr Val Leu Asp Val Asn Val Phe
1 5 10 15
gat gag ctc cgc atc ggc ctg gcc acc gcc gac gac atc cgc cgt tgg 96
Asp Glu Leu Arg Ile Gly Leu Ala Thr Ala Asp Asp Ile Arg Arg Trp
20 25 30
tcc aag ggt gag gtc aag aag ccg gag acc atc aac tac cga acc ctc 144
Ser Lys Gly Glu Val Lys Lys Pro Glu Thr Ile Asn Tyr Arg Thr Leu
35 40 45
aag cct gag aag gac ggt ctg ttc tgc gag cgt atc ttc ggt cca act 192
Lys Pro Glu Lys Asp Gly Leu Phe Cys Glu Arg Ile Phe Gly Pro Thr
50 55 60
cgc gac tgg gag tgc gcc tgc ggt aag tac aag cgt gtc cgc tac aag 240
Arg Asp Trp Glu Cys Ala Cys Gly Lys Tyr Lys Arg Val Arg Tyr Lys
65 70 75 80
ggc atc atc tgt gaa cgc tgt ggc gtt gag gtc acc aag tcc aag gtg 288
Gly Ile Ile Cys Glu Arg Cys Gly Val Glu Val Thr Lys Ser Lys Val
85 90 95
cgc cgt gag cgc atg gga cac att gag ctc gct gca cca gta acc cac 336
Arg Arg Glu Arg Met Gly His Ile Glu Leu Ala Ala Pro Val Thr His
100 105 110
att tgg tac ttc aag ggc gtt cca tca cgc ctc ggc tac ctt ttg gac 384
Ile Trp Tyr Phe Lys Gly Val Pro Ser Arg Leu Gly Tyr Leu Leu Asp
115 120 125
ctt gct cca aag gac ctg gac ctc atc atc tac ttc ggt gcg aac atc 432
Leu Ala Pro Lys Asp Leu Asp Leu Ile Ile Tyr Phe Gly Ala Asn Ile
130 135 140
atc acc agc gtg gac gaa gag gct cgc cac agc gac cag acc act ctt 480
Ile Thr Ser Val Asp Glu Glu Ala Arg His Ser Asp Gln Thr Thr Leu
145 150 155 160
gag gca gaa atg ctt ctg gag aag aag gac gtt gag gca gac gca gag 528
Glu Ala Glu Met Leu Leu Glu Lys Lys Asp Val Glu Ala Asp Ala Glu
165 170 175
tct gac att gct gag cgt gct gaa aag ctc gaa gag gat ctt gct gaa 576
Ser Asp Ile Ala Glu Arg Ala Glu Lys Leu Glu Glu Asp Leu Ala Glu
180 185 190
ctt gag gca gct ggc gct aag gcc gac gct cgc cgc aag gtt cag gct 624
Leu Glu Ala Ala Gly Ala Lys Ala Asp Ala Arg Arg Lys Val Gln Ala
195 200 205
gct gcc gat aag gaa atg cag cac atc cgt gag cgt gca cag cgc gaa 672
Ala Ala Asp Lys Glu Met Gln His Ile Arg Glu Arg Ala Gln Arg Glu
210 215 220
atc gat cgt ctc gat gag gtc tgg cag acc ttc atc aag ctt gct cca 720
Ile Asp Arg Leu Asp Glu Val Trp Gln Thr Phe Ile Lys Leu Ala Pro
225 230 235 240
aag cag atg atc cgc gat gag aag ctc tac gat gaa ctg atc gac cgc 768
Lys Gln Met Ile Arg Asp Glu Lys Leu Tyr Asp Glu Leu Ile Asp Arg
245 250 255
tac gag gat tac ttc acc ggt ggt atg ggt gca gag tcc att gag gct 816
Tyr Glu Asp Tyr Phe Thr Gly Gly Met Gly Ala Glu Ser Ile Glu Ala
260 265 270
ttg atc cag aac ttc gac ctt gat gct gag gct gaa gag ctg cgc gac 864
Leu Ile Gln Asn Phe Asp Leu Asp Ala Glu Ala Glu Glu Leu Arg Asp
275 280 285
atc atc aac aat ggc aag ggc cag aag aag atg cgt gca ctg aag cgc 912
Ile Ile Asn Asn Gly Lys Gly Gln Lys Lys Met Arg Ala Leu Lys Arg
290 295 300
ctg aag gtt gtt gca gcc ttc cag cgt tcc ggc aac gat cct gcc ggc 950
Leu Lys Val Val Ala Ala Phe Gln Arg Ser Gly Asn Asp Pro Ala Gly
305 310 315 320
atg gtt ttg aac gcg atc cca gtg atc cca cca gag ctt cgc cca atg 1008
Met Val Leu Asn Ala Ile Pro Val Ile Pro Pro Glu Leu Arg Pro Met
325 330 335
gtt cag ctt gac ggt ggt cgc ttc gct acc tcc gac ttg aac gac ctt 1056
Val Gln Leu Asp Gly Gly Arg Phe Ala Thr Ser Asp Leu Asn Asp Leu
340 345 350
tac cgt cgt gtg atc aac cgc aac aac cgt ctg aag cgc atg att gag 1104
Tyr Arg Arg Val Ile Asn Arg Asn Asn Arg Leu Lys Arg Met Ile Glu
355 360 365
ctc ggt gca cct gag atc atc gtg aac aac gag aag cgc atg ctg cag 1152
Leu Gly Ala Pro Glu Ile Ile Val Asn Asn Glu Lys Arg Met Leu Gln
370 375 380
gaa tct gtg gac gcg ctg ttc gac aac ggt cgt cgt ggt cgc cca gtt 1200
Glu Ser Val Asp Ala Leu Phe Asp Asn Gly Arg Arg Gly Arg Pro Val
385 390 395 400
acc gga ccg ggt aac cgt ccg ctg aag tct ctg tct gac ttg ctc aag 1248
Thr Gly Pro Gly Asn Arg Pro Leu Lys Ser Leu Ser Asp Leu Leu Lys
405 410 415
ggc aag caa ggc cgt ttc cgt cag aac ctt ctg ggt aag cgt gtt gac 1296
Gly Lys Gln Gly Arg Phe Arg Gln Asn Leu Leu Gly Lys Arg Val Asp
420 425 430
tac tct ggt cgt tcc gta att atc gtt ggt cct cag ctg cgc ctc cac 1344
Tyr Ser Gly Arg Ser Val Ile Ile Val Gly Pro Gln Leu Arg Leu His
435 440 445
gaa tgt ggt ctg cct aag ctg atg gct ctc gag ctc ttc aag cct ttc 1392
Glu Cys Gly Leu Pro Lys Leu Met Ala Leu Glu Leu Phe Lys Pro Phe
450 455 460
gtc atg aag cgc ttg gtg gag aac gag tac gca cag aac atc aag tct 1440
Val Met Lys Arg Leu Val Glu Asn Glu Tyr Ala Gln Asn Ile Lys Ser
465 470 475 480
gca aag cgc atg gtt gag cgt cag cgc cct gag gtg tgg gac gtc ctc 1488
Ala Lys Arg Met Val Glu Arg Gln Arg Pro Glu Val Trp Asp Val Leu
485 490 495
gaa gag gcc atc tct gag cac cca gtg atg ctg aac cgt gca cca acc 1536
Glu Glu Ala Ile Ser Glu His Pro Val Met Leu Asn Arg Ala Pro Thr
500 505 510
ctg cac cgc ttg ggc att cag gct ttc gag cct gtc ctt gtt gag ggt 1584
Leu His Arg Leu Gly Ile Gln Ala Phe Glu Pro Val Leu Val Glu Gly
515 520 525
aag gct att cag ctg cac cca ctt gct tgt gaa gct ttc aac gcc gac 1632
Lys Ala Ile Gln Leu His Pro Leu Ala Cys Glu Ala Phe Asn Ala Asp
530 535 540
ttc gat ggt gac cag atg gca gtt cac ctg ccg ctg tcc gct gaa gct 1680
Phe Asp Gly Asp Gln Met Ala Val His Leu Pro Leu Ser Ala Glu Ala
545 550 555 560
cag gct gag gct cgc gtg ctg atg ctt gca tcc aac aac att ttg tcc 1728
Gln Ala Glu Ala Arg Val Leu Met Leu Ala Ser Asn Asn Ile Leu Ser
565 570 575
cca gct tcc ggt aag cct ttg gct atg cct cgt ctg gat atg gtg acc 1776
Pro Ala Ser Gly Lys Pro Leu Ala Met Pro Arg Leu Asp Met Val Thr
580 585 590
ggt ctg tac tac ctg act ctg gag aag tct tcc gag gag ttc ggt gga 1824
Gly Leu Tyr Tyr Leu Thr Leu Glu Lys Ser Ser Glu Glu Phe Gly Gly
595 600 605
cag ggc gct tac cag cct gca gat gaa aac ggt cct gaa aag ggc gtg 1872
Gln Gly Ala Tyr Gln Pro Ala Asp Glu Asn Gly Pro Glu Lys Gly Val
610 615 620
tat tcc tca ctg gca gaa gca atc atg gct tat gac cgt ggt gta ctt 1920
Tyr Ser Ser Leu Ala Glu Ala Ile Met Ala Tyr Asp Arg Gly Val Leu
625 630 635 640
ggc ctg cag gcc cca gtt cgc atc cgt ttg aac cac ctg cgc cca cca 1968
Gly Leu Gln Ala Pro Val Arg Ile Arg Leu Asn His Leu Arg Pro Pro
645 650 655
gct gag gta gaa gca gag cag ttc cca gat gga tgg aac cag ggc gag 2016
Ala Glu Val Glu Ala Glu Gln Phe Pro Asp Gly Trp Asn Gln Gly Glu
660 665 670
act tgg ttg gct cac acc acc ttg ggt cgc gtt atg ttc aac gag atc 2064
Thr Trp Leu Ala His Thr Thr Leu Gly Arg Val Met Phe Asn Glu Ile
675 680 685
ctg cct tgg aac tac cca tac ctt gag ggc gtt atg gtc cgt aag ggt 2112
Leu Pro Trp Asn Tyr Pro Tyr Leu Glu Gly Val Met Val Arg Lys Gly
690 695 700
ggc ggc tcc gac aag atc atg ctt ggc gac gta gtc aat gac ctc gct 2160
Gly Gly Ser Asp Lys Ile Met Leu Gly Asp Val Val Asn Asp Leu Ala
705 710 715 720
gct aag tac cca atg atc acc gtg gct cag acc atg gac aag atg aag 2208
Ala Lys Tyr Pro Met Ile Thr Val Ala Gln Thr Met Asp Lys Met Lys
725 730 735
gat gct ggc ttc tac tgg tca acc cgt tcc ggt gtg acc atc gct atg 2256
Asp Ala Gly Phe Tyr Trp Ser Thr Arg Ser Gly Val Thr Ile Ala Met
740 745 750
tct gac gtt ttg gtt ctt cct aac aag gaa gaa atg ctg gac cgc tac 2304
Ser Asp Val Leu Val Leu Pro Asn Lys Glu Glu Met Leu Asp Arg Tyr
755 760 765
gag gaa tct gca cgc cag atc gaa gtt aag tac aac cgc ggt aag ctc 2352
Glu Glu Ser Ala Arg Gln Ile Glu Val Lys Tyr Asn Arg Gly Lys Leu
770 775 780
acc ggc cgt gag cgc tac gac cgt ctg gtc gag ctg tgg aag gac gca 2400
Thr Gly Arg Glu Arg Tyr Asp Arg Leu Val Glu Leu Trp Lys Asp Ala
785 790 795 800
act gac gag gtt gga cag gct gtc gag gat ctg tac cca gac gac aac 2448
Thr Asp Glu Val Gly Gln Ala Val Glu Asp Leu Tyr Pro Asp Asp Asn
805 810 815
cca att cca atg atc gtg aag tct ggt gct gcc ggt aac atg cgt cag 2496
Pro Ile Pro Met Ile Val Lys Ser Gly Ala Ala Gly Asn Met Arg Gln
820 825 830
atc tgg acc ctt gct ggt atg aag ggc atg gtt gtg aac tcg aag ggt 2544
Ile Trp Thr Leu Ala Gly Met Lys Gly Met Val Val Asn Ser Lys Gly
835 840 845
gac tac atc acc cgc ccg atc aag act tcc ttc cgt gaa ggc ttg acc 2592
Asp Tyr Ile Thr Arg Pro Ile Lys Thr Ser Phe Arg Glu Gly Leu Thr
850 855 860
gtt ctc gag tac ttc aac aac tcc cac ggt tcc cgt aag ggc ctg gcc 2640
Val Leu Glu Tyr Phe Asn Asn Ser His Gly Ser Arg Lys Gly Leu Ala
865 870 875 880
gat acc gcg ctg cgt acc gct gac tcc ggt tac ctg acc cgt cgt ctt 2688
Asp Thr Ala Leu Arg Thr Ala Asp Ser Gly Tyr Leu Thr Arg Arg Leu
885 890 895
gtt gac gtc gct cag gac gtc atc gtg cgt gtt gag gac tgt ggc acc 2736
Val Asp Val Ala Gln Asp Val Ile Val Arg Val Glu Asp Cys Gly Thr
900 905 910
cgc cag ggt gtt cgc gtt cct gtc gct gct gag gtt ctg gat gca act 2784
Arg Gln Gly Val Arg Val Pro Val Ala Ala Glu Val Leu Asp Ala Thr
915 920 925
ggt gct gtc acc ggc tac acc cgc cat gac ctg atc gag act tct gtc 2832
Gly Ala Val Thr Gly Tyr Thr Arg His Asp Leu Ile Glu Thr Ser Val
930 935 940
tcc ggt cgt gtt ctg gct ggc gat gca acc aac gct gca ggc gag gtt 2880
Ser Gly Arg Val Leu Ala Gly Asp Ala Thr Asn Ala Ala Gly Glu Val
945 950 955 960
gtg ctt gct gct ggt acc gac ctg acc gag ctc aac att gac ctt ctg 2928
Val Leu Ala Ala Gly Thr Asp Leu Thr Glu Leu Asn Ile Asp Leu Leu
965 970 975
gtc gag gct ggc atc aag gac gtc aag gtt cgt tcc gta ctt acc tgc 2976
Val Glu Ala Gly Ile Lys Asp Val Lys Val Arg Ser Val Leu Thr Cys
980 985 990
cag acc cca acc ggt gtt tgt gct aag tgc tac ggc aag tcc atg gct 3024
Gln Thr Pro Thr Gly Val Cys Ala Lys Cys Tyr Gly Lys Ser Met Ala
995 1000 1005
tcc ggc cag cag gtt gat atc gga gag gct gtc ggt att gtt gct gca 3072
Ser Gly Gln Gln Val Asp Ile Gly Glu Ala Val Gly Ile Val Ala Ala
1010 1015 1020
cag tcc att ggt gag cct ggt acc cag ctg acc atg cgt acc ttc cac 3120
Gln Ser Ile Gly Glu Pro Gly Thr Gln Leu Thr Met Arg Thr Phe His
1025 1030 1035 1040
cag ggt ggt gtc ggt ggc gat att acc ggc ggt ctg cct cgt gtt cag 3168
Gln Gly Gly Val Gly Gly Asp Ile Thr Gly Gly Leu Pro Arg Val Gln
1045 1050 1055
gag ctg ttt gag gca cgt gtt cct aag aac tgt gca cca att gct tct 3216
Glu Leu Phe Glu Ala Arg Val Pro Lys Asn Cys Ala Pro Ile Ala Ser
1060 1065 1070
gtt gaa ggt gtt atc cac ctt gag gat gaa ggc aac ttc tac act ctg 3264
Val Glu Gly Val Ile His Leu Glu Asp Glu Gly Asn Phe Tyr Thr Leu
1075 1080 1085
acc atc gtt cct gac gat ggc tcc gac aac gtt gtc tac gag aag ctg 3312
Thr Ile Val Pro Asp Asp Gly Ser Asp Asn Val Val Tyr Glu Lys Leu
1090 1095 1100
tcc aag cga cag ggt ctt gca tcc act cgc gtg gct atg gag tcc aac 3360
Ser Lys Arg Gln Gly Leu Ala Ser Thr Arg Val Ala Met Glu Ser Asn
1105 1110 1115 1120
gct ggt gcg ttc att gag cgc acc ttg acc gaa ggt gac cgc gtc acc 3408
Ala Gly Ala Phe Ile Glu Arg Thr Leu Thr Glu Gly Asp Arg Val Thr
1125 1130 1135
gtt ggt cag cgt ctg ctc cgt ggt gca gct gat cca cac gac gtg ctc 3456
Val Gly Gln Arg Leu Leu Arg Gly Ala Ala Asp Pro His Asp Val Leu
1140 1145 1150
gag atc ctc ggt cgc cgt ggt gtg gag cag cac ctc atc gat gag gtg 3504
Glu Ile Leu Gly Arg Arg Gly Val Glu Gln His Leu Ile Asp Glu Val
1155 1160 1165
cag gct gtt tac cgt gca cag ggt gtg gcc atc cac gac aag cac atc 3552
Gln Ala Val Tyr Arg Ala Gln Gly Val Ala Ile His Asp Lys His Ile
1170 1175 1180
gaa atc atc att cgt cag atg ctg cgt cgc ggt acc gtc att gag tcc 3600
Glu Ile Ile Ile Arg Gln Met Leu Arg Arg Gly Thr Val Ile Glu Ser
1185 1190 1195 1200
ggt tcc acc gag ttc ctt cct ggt tct ttg gtt gac ctc tct gag gcg 3648
Gly Ser Thr Glu Phe Leu Pro Gly Ser Leu Val Asp Leu Ser Glu Ala
1205 1210 1215
aag ctg gct aac tct gag gca atc ggt gcg ggc ggt cag cct gca gag 3696
Lys Leu Ala Asn Ser Glu Ala Ile Gly Ala Gly Gly Gln Pro Ala Glu
1220 1225 1230
ctg cgt tct gag atc atg ggt atc acc aag gcc tct ctc gca act gag 3744
Leu Arg Ser Glu Ile Met Gly Ile Thr Lys Ala Ser Leu Ala Thr Glu
1235 1240 1245
tct tgg ctg tct gca gcg tcc ttc cag gag acc act cgt gtc ctg act 3792
Ser Trp Leu Ser Ala Ala Ser Phe Gln Glu Thr Thr Arg Val Leu Thr
1250 1255 1260
gat gct gct atc aac aag cgc tcc gat aag ctc atc ggc ctg aag gag 3840
Asp Ala Ala Ile Asn Lys Arg Ser Asp Lys Leu Ile Gly Leu Lys Glu
1265 1270 1275 1280
aac gtg atc atc ggt aag ctg atc cca gct ggt act ggt att tcc cgt 3888
Asn Val Ile Ile Gly Lys Leu Ile Pro Ala Gly Thr Gly Ile Ser Arg
1285 1290 1295
tac cgc aac atc tcc atc aag cca acc gag gct gct cgc aac gcc gca 3936
Tyr Arg Asn Ile Ser Ile Lys Pro Thr Glu Ala Ala Arg Asn Ala Ala
1300 1305 1310
tac tcg atc cca act tat ggt gag tcg att tac ggt gac gat gga ttc 3984
Tyr Ser Ile Pro Thr Tyr Gly Glu Ser Ile Tyr Gly Asp Asp Gly Phe
1315 1320 1325
ggt gag ttc acc ggc gca tcc gtc cca ttg gat gag gct ttc 4026
Gly Glu Phe Thr Gly Ala Ser Val Pro Leu Asp Glu Ala Phe
1330 1335 1340
<210>88
<211>1342
<212>PRT
<213>谷氨酸棒状杆菌
<400>88
Met Phe Trp Gly Glu Arg Glu Phe Tyr Val Leu Asp Val Asn Val Phe
1 5 10 15
Asp Glu Leu Arg Ile Gly Leu Ala Thr Ala Asp Asp Ile Arg Arg Trp
20 25 30
Ser Lys Gly Glu Val Lys Lys Pro Glu Thr Ile Asn Tyr Arg Thr Leu
35 40 45
Lys Pro Glu Lys Asp Gly Leu Phe Cys Glu Arg Ile Phe Gly Pro Thr
50 55 60
Arg Asp Trp Glu Cys Ala Cys Gly Lys Tyr Lys Arg Val Arg Tyr Lys
65 70 75 80
Gly Ile Ile Cys Glu Arg Cys Gly Val Glu Val Thr Lys Ser Lys Val
85 90 95
Arg Arg Glu Arg Met Gly His Ile Glu Leu Ala Ala Pro Val Thr His
100 105 110
Ile Trp Tyr Phe Lys Gly Val Pro Ser Arg Leu Gly Tyr Leu Leu Asp
115 120 125
Leu Ala Pro Lys Asp Leu Asp Leu Ile Ile Tyr Phe Gly Ala Asn Ile
130 135 140
Ile Thr Ser Val Asp Glu Glu Ala Arg His Ser Asp Gln Thr Thr Leu
145 150 155 160
Glu Ala Glu Met Leu Leu Glu Lys Lys Asp Val Glu Ala Asp Ala Glu
165 170 175
Ser Asp Ile Ala Glu Arg Ala Glu Lys Leu Glu Glu Asp Leu Ala Glu
180 185 190
Leu Glu Ala Ala Gly Ala Lys Ala Asp Ala Arg Arg Lys Val Gln Ala
195 200 205
Ala Ala Asp Lys Glu Met Gln His Ile Arg Glu Arg Ala Gln Arg Glu
210 215 220
Ile Asp Arg Leu Asp Glu Val Trp Gln Thr Phe Ile Lys Leu Ala Pro
225 230 235 240
Lys Gln Met Ile Arg Asp Glu Lys Leu Tyr Asp Glu Leu Ile Asp Arg
245 250 255
Tyr Glu Asp Tyr Phe Thr Gly Gly Met Gly Ala Glu Ser Ile Glu Ala
260 265 270
Leu Ile Gln Asn Phe Asp Leu Asp Ala Glu Ala Glu Glu Leu Arg Asp
275 280 285
Ile Ile Asn Asn Gly Lys Gly Gln Lys Lys Met Arg Ala Leu Lys Arg
290 295 300
Leu Lys Val Val Ala Ala Phe Gln Arg Ser Gly Asn Asp Pro Ala Gly
305 310 315 320
Met Val Leu Asn Ala Ile Pro Val Ile Pro Pro Glu Leu Arg Pro Met
325 330 335
Val Gln Leu Asp Gly Gly Arg Phe Ala Thr Ser Asp Leu Asn Asp Leu
340 345 350
Tyr Arg Arg Val Ile Asn Arg Asn Asn Arg Leu Lys Arg Met Ile Glu
355 360 365
Leu Gly Ala Pro Glu Ile Ile Val Asn Asn Glu Lys Arg Met Leu Gln
370 375 380
Glu Ser Val Asp Ala Leu Phe Asp Asn Gly Arg Arg Gly Arg Pro Val
385 390 395 400
Thr Gly Pro Gly Asn Arg Pro Leu Lys Ser Leu Ser Asp Leu Leu Lys
405 410 415
Gly Lys Gln Gly Arg Phe Arg Gln Asn Leu Leu Gly Lys Arg Val Asp
420 425 430
Tyr Ser Gly Arg Ser Val Ile Ile Val Gly Pro Gln Leu Arg Leu His
435 440 445
Glu Cys Gly Leu Pro Lys Leu Met Ala Leu Glu Leu Phe Lys Pro Phe
450 455 460
Val Met Lys Arg Leu Val Glu Asn Glu Tyr Ala Gln Asn Ile Lys Ser
465 470 475 480
Ala Lys Arg Met Val Glu Arg Gln Arg Pro Glu Val Trp Asp Val Leu
485 490 495
Glu Glu Ala Ile Ser Glu His Pro Val Met Leu Asn Arg Ala Pro Thr
500 505 510
Leu His Arg Leu Gly Ile Gln Ala Phe Glu Pro Val Leu Val Glu Gly
515 520 525
Lys Ala Ile Gln Leu His Pro Leu Ala Cys Glu Ala Phe Asn Ala Asp
530 535 540
Phe Asp Gly Asp Gln Met Ala Val His Leu Pro Leu Ser Ala Glu Ala
545 550 555 560
Gln Ala Glu Ala Arg Val Leu Met Leu Ala Ser Asn Asn Ile Leu Ser
565 570 575
Pro Ala Ser Gly Lys Pro Leu Ala Met Pro Arg Leu Asp Met Val Thr
580 585 590
Gly Leu Tyr Tyr Leu Thr Leu Glu Lys Ser Ser Glu Glu Phe Gly Gly
595 600 605
Gln Gly Ala Tyr Gln Pro Ala Asp Glu Asn Gly Pro Glu Lys Gly Val
610 615 620
Tyr Ser Ser Leu Ala Glu Ala Ile Met Ala Tyr Asp Arg Gly Val Leu
625 630 635 640
Gly Leu Gln Ala Pro Val Arg Ile Arg Leu Asn His Leu Arg Pro Pro
645 650 655
Ala Glu Val Glu Ala Glu Gln Phe Pro Asp Gly Trp Asn Gln Gly Glu
660 665 670
Thr Trp Leu Ala His Thr Thr Leu Gly Arg Val Met Phe Asn Glu Ile
675 680 685
Leu Pro Trp Asn Tyr Pro Tyr Leu Glu Gly Val Met Val Arg Lys Gly
690 695 700
Gly Gly Ser Asp Lys Ile Met Leu Gly Asp Val Val Asn Asp Leu Ala
705 710 715 720
Ala Lys Tyr Pro Met Ile Thr Val Ala Gln Thr Met Asp Lys Met Lys
725 730 735
Asp Ala Gly Phe Tyr Trp Ser Thr Arg Ser Gly Val Thr Ile Ala Met
740 745 750
Ser Asp Val Leu Val Leu Pro Asn Lys Glu Glu Met Leu Asp Arg Tyr
755 760 765
Glu Glu Ser Ala Arg Gln Ile Glu Val Lys Tyr Asn Arg Gly Lys Leu
770 775 780
Thr Gly Arg Glu Arg Tyr Asp Arg Leu Val Glu Leu Trp Lys Asp Ala
785 790 795 800
Thr Asp Glu Val Gly Gln Ala Val Glu Asp Leu Tyr Pro Asp Asp Asn
805 810 815
Pro Ile Pro Met Ile Val Lys Ser Gly Ala Ala Gly Asn Met Arg Gln
820 825 830
Ile Trp Thr Leu Ala Gly Met Lys Gly Met Val Val Asn Ser Lys Gly
835 840 845
Asp Tyr Ile Thr Arg Pro Ile Lys Thr Ser Phe Arg Glu Gly Leu Thr
850 855 860
Val Leu Glu Tyr Phe Asn Asn Ser His Gly Ser Arg Lys Gly Leu Ala
865 870 875 880
Asp Thr Ala Leu Arg Thr Ala Asp Ser Gly Tyr Leu Thr Arg Arg Leu
885 890 895
Val Asp Val Ala Gln Asp Val Ile Val Arg Val Glu Asp Cys Gly Thr
900 905 910
Arg Gln Gly Val Arg Val Pro Val Ala Ala Glu Val Leu Asp Ala Thr
915 920 925
Gly Ala Val Thr Gly Tyr Thr Arg His Asp Leu Ile Glu Thr Ser Val
930 935 940
Ser Gly Arg Val Leu Ala Gly Asp Ala Thr Asn Ala Ala Gly Glu Val
945 950 955 960
Val Leu Ala Ala Gly Thr Asp Leu Thr Glu Leu Asn Ile Asp Leu Leu
965 970 975
Val Glu Ala Gly Ile Lys Asp Val Lys Val Arg Ser Val Leu Thr Cys
980 985 990
Gln Thr Pro Thr Gly Val Cys Ala Lys Cys Tyr Gly Lys Ser Met Ala
995 1000 1005
Ser Gly Gln Gln Val Asp Ile Gly Glu Ala Val Gly Ile Val Ala Ala
1010 1015 1020
Gln Ser Ile Gly Glu Pro Gly Thr Gln Leu Thr Met Arg Thr Phe His
1025 1030 1035 1040
Gln Gly Gly Val Gly Gly Asp Ile Thr Gly Gly Leu Pro Arg Val Gln
1045 1050 1055
Glu Leu Phe Glu Ala Arg Val Pro Lys Asn Cys Ala Pro Ile Ala Ser
1060 1065 1070
Val Glu Gly Val Ile His Leu Glu Asp Glu Gly Asn Phe Tyr Thr Leu
1075 1080 1085
Thr Ile Val Pro Asp Asp Gly Ser Asp Asn Val Val Tyr Glu Lys Leu
1090 1095 1100
Ser Lys Arg Gln Gly Leu Ala Ser Thr Arg Val Ala Met Glu Ser Asn
1105 1110 1115 1120
Ala Gly Ala Phe Ile Glu Arg Thr Leu Thr Glu Gly Asp Arg Val Thr
1125 1130 1135
Val Gly Gln Arg Leu Leu Arg Gly Ala Ala Asp Pro His Asp Val Leu
1140 1145 1150
Glu Ile Leu Gly Arg Arg Gly Val Glu Gln His Leu Ile Asp Glu Val
1155 1160 1165
Gln Ala Val Tyr Arg Ala Gln Gly Val Ala Ile His Asp Lys His Ile
1170 1175 1180
Glu Ile Ile Ile Arg Gln Met Leu Arg Arg Gly Thr Val Ile Glu Ser
1185 1190 1195 1200
Gly Ser Thr Glu Phe Leu Pro Gly Ser Leu Val Asp Leu Ser Glu Ala
1205 1210 1215
Lys Leu Ala Asn Ser Glu Ala Ile GlV Ala Gly Gly Gln Pro Ala Glu
1220 1225 1230
Leu Arg Ser Glu Ile Met Gly Ile Thr Lys Ala Ser Leu Ala Thr Glu
1235 1240 1245
Ser Trp Leu Ser Ala Ala Ser Phe Gln Glu Thr Thr Arg Val Leu Thr
1250 1255 1260
Asp Ala Ala Ile Asn Lys Arg Ser Asp Lys Leu Ile Gly Leu Lys Glu
1265 1270 1275 1280
Asn Val Ile Ile Gly Lys Leu Ile Pro Ala Gly Thr Gly Ile Ser Arg
1285 1290 1295
Tyr Arg Asn Ile Ser Ile Lys Pro Thr Glu Ala Ala Arg Asn Ala Ala
1300 1305 1310
Tyr Ser Ile Pro Thr Tyr Gly Glu Ser Ile Tyr Gly Asp Asp Gly Phe
1315 1320 1325
Gly Glu Phe Thr Gly Ala Ser Val Pro Leu Asp Glu Ala Phe
1330 1335 1340
<210>89
<211>840
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(840)
<223>RXA07019
<400>89
atg gct att cgt aag tac aag ccg aca acc ccg ggt cgc cgc gca agc 48
Met Ala Ile Arg Lys Tyr Lys Pro Thr Thr Pro Gly Arg Arg Ala Ser
1 5 10 15
tcc gtt tcc atg ttc acg gag atc acc cgt tcg acc cct gag aag tca 96
Ser Val Ser Met Phe Thr Glu Ile Thr Arg Ser Thr Pro Glu Lys Ser
20 25 30
ctt ctc cgc cca ctg agc aag acc ggc gga cgt aac tct cac ggc cac 144
Leu Leu Arg Pro Leu Ser Lys Thr Gly Gly Arg Asn Ser His Gly His
35 40 45
atc acc acc cgt cac cgc ggt ggt gga cac aag cgc cgc tac cgc gtc 192
Ile Thr Thr Arg His Arg Gly Gly Gly His Lys Arg Arg Tyr Arg Val
50 55 60
atc gac ttc cgt cgt aac gac aag gat ggc gta ttg gcg aag gtc gct 240
Ile Asp Phe Arg Arg Asn Asp Lys Asp Gly Val Leu Ala Lys Val Ala
65 70 75 80
cac atc gag tac gac cca aac cgt acc gct aac att gca ctg ctt cac 288
His Ile Glu Tyr Asp Pro Asn Arg Thr Ala Asn Ile Ala Leu Leu His
85 90 95
tac ttc gat ggc gag aag cgt tac atc ctc gca ccg aag ggc ctg acc 336
Tyr Phe Asp Gly Glu Lys Arg Tyr Ile Leu Ala Pro Lys Gly Leu Thr
100 105 110
cag ggc acc gtt atc gag tcc ggc gct gca gcc gac atc aag gtt ggt 384
Gln Gly Thr Val Ile Glu Ser Gly Ala Ala Ala Asp Ile Lys Val Gly
115 120 125
aac aac ctg cca ctg cgt aac atc ccg act ggt acc acc atc cac aac 432
Asn Asn Leu Pro Leu Arg Asn Ile Pro Thr Gly Thr Thr Ile His Asn
130 135 140
gtg gag ttg aag cca ggc gca ggt gca aag ctg gca cgt tcc gct gga 480
Val Glu Leu Lys Pro Gly Ala Gly Ala Lys Leu Ala Arg Ser Ala Gly
145 150 155 160
gct tcc atc cag ctt ctt ggt aag gaa ggc tcc tac gca gtt ctg cgt 528
Ala Ser Ile Gln Leu Leu Gly Lys Glu Gly Ser Tyr Ala Val Leu Arg
165 170 175
atg cca tcc tcc gag atc cga cgc gta gac atc cgc tgc cgc gcg act 576
Met Pro Ser Ser Glu Ile Arg Arg Val Asp Ile Arg Cys Arg Ala Thr
180 185 190
gtt ggt gag gtc ggc aac gcc gag cag atc aac att cgt tgg ggt aaa 624
Val Gly Glu Val Gly Asn Ala Glu Gln Ile Asn Ile Arg Trp Gly Lys
195 200 205
gct ggt cgt atg cgt tgg aag ggc tgg cgc cca acc gtc cgt ggt gtc 672
Ala Gly Arg Met Arg Trp Lys Gly Trp Arg Pro Thr Val Arg Gly Val
210 215 220
gtt atg aac ccg gtc gac cac cca cac ggt ggt ggt gaa ggt aag act 720
Val Met Asn Pro Val Asp His Pro His Gly Gly Gly Glu Gly Lys Thr
225 230 235 240
tct ggt ggt cgc cac cca gtc tcc cca tgg gga cag aag gaa ggc cgc 768
Ser Gly Gly Arg His Pro Val Ser Pro Trp Gly Gln Lys Glu Gly Arg
245 250 255
acc cgc aag cct aag cgt tac agc gat gac atg atc gtt cgt cgc cgt 816
Thr Arg Lys Pro Lys Arg Tyr Ser Asp Asp Met Ile Val Arg Arg Arg
260 265 270
cgt gct aac aag aac aag aag cgc 840
Arg Ala Asn Lys Asn Lys Lys Arg
275 280
<210>90
<211>280
<212>PRT
<213>谷氨酸棒状杆菌
<400>90
Met Ala Ile Arg Lys Tyr Lys Pro Thr Thr Pro Gly Arg Arg Ala Ser
1 5 10 15
Ser Val Ser Met Phe Thr Glu Ile Thr Arg Ser Thr Pro Glu Lys Ser
20 25 30
Leu Leu Arg Pro Leu Ser Lys Thr Gly Gly Arg Asn Ser His Gly His
35 40 45
Ile Thr Thr Arg His Arg Gly Gly Gly His Lys Arg Arg Tyr Arg Val
50 55 60
Ile Asp Phe Arg Arg Asn Asp Lys Asp Gly Val Leu Ala Lys Val Ala
65 70 75 80
His Ile Glu Tyr Asp Pro Asn Arg Thr Ala Asn Ile Ala Leu Leu His
85 90 95
Tyr Phe Asp Gly Glu Lys Arg Tyr Ile Leu Ala Pro Lys Gly Leu Thr
100 105 110
Gln Gly Thr Val Ile Glu Ser Gly Ala Ala Ala Asp Ile Lys Val Gly
115 120 125
Asn Asn Leu Pro Leu Arg Asn Ile Pro Thr Gly Thr Thr Ile His Asn
130 135 140
Val Glu Leu Lys Pro Gly Ala Gly Ala Lys Leu Ala Arg Ser Ala Gly
145 150 155 160
Ala Ser Ile Gln Leu Leu Gly Lys Glu Gly Ser Tyr Ala Val Leu Arg
165 170 175
Met Pro Ser Ser Glu Ile Arg Arg Val Asp Ile Arg Cys Arg Ala Thr
180 185 190
Val Gly Glu Val Gly Asn Ala Glu Gln Ile Asn Ile Arg Trp Gly Lys
195 200 205
Ala Gly Arg Met Arg Trp Lys Gly Trp Arg Pro Thr Val Arg Gly Val
210 215 220
Val Met Asn Pro Val Asp His Pro His Gly Gly Gly Glu Gly Lys Thr
225 230 235 240
Ser Gly Gly Arg His Pro Val Ser Pro Trp Gly Gln Lys Glu Gly Arg
245 250 255
Thr Arg Lys Pro Lys Arg Tyr Ser Asp Asp Met Ile Val Arg Arg Arg
260 265 270
Arg Ala Asn Lys Asn Lys Lys Arg
275 280
<210>91
<211>1089
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(1089)
<223>RXA07020
<400>91
atg gcg ggg tta gtt gac tta aca ata aac atg agg gaa ggg ctg gac 48
Met Ala Gly Leu Val Asp Leu Thr Ile Asn Met Arg Glu Gly Leu Asp
1 5 10 15
tac acg gtg tcc gaa att cag ttg acc gaa gcc agt ttg aac gag gcg 96
Tyr Thr Val Ser Glu Ile Gln Leu Thr Glu Ala Ser Leu Asn Glu Ala
20 25 30
gcc gac gcc gcg atc aag gct ttc gac ggt gca caa aac cta gat gaa 144
Ala Asp Ala Ala Ile Lys Ala Phe Asp Gly Ala Gln Asn Leu Asp Glu
35 40 45
ctc gca gca cta cgc cgc gat cac ttg ggt gat gcc gca cct att ccg 192
Leu Ala Ala Leu Arg Arg Asp His Leu Gly Asp Ala Ala Pro Ile Pro
50 55 60
cag gca cga cgc tca ttg gga act att cct aaa gat cag cgc aag gat 240
Gln Ala Arg Arg Ser Leu Gly Thr Ile Pro Lys Asp Gln Arg Lys Asp
65 70 75 80
gcg ggc cgc ttt gtc aac atg gca ctt ggt cgc gca gaa aag cac ttt 288
Ala Gly Arg Phe Val Asn Met Ala Leu Gly Arg Ala Glu Lys His Phe
85 90 95
gct cag gtc aag gtt gtt ttg gaa gaa aag cgc aac gca gaa gtc cta 336
Ala Gln Val Lys Val Val Leu Glu Glu Lys Arg Asn Ala Glu Val Leu
100 105 110
gag ctt gag cgc gtt gac gtc acc gtt cca acc acc cgc gag cag gtt 384
Glu Leu Glu Arg Val Asp Val Thr Val Pro Thr Thr Arg Glu Gln Val
115 120 125
ggt gcg ctg cac ccc atc acg atc ctc aac gag cag atc gca gac atc 432
Gly Ala Leu His Pro Ile Thr Ile Leu Asn Glu Gln Ile Ala Asp Ile
130 135 140
ttc gtg ggc atg ggc tgg gaa atc gcc gag ggc cca gag gtg gaa gca 430
Phe Val Gly Met Gly Trp Glu Ile Ala Glu Gly Pro Glu Val Glu Ala
145 150 155 160
gaa tac ttc aac ttc gac gca ctg aac ttc ctg cct gat cac cca gct 528
Glu Tyr Phe Asn Phe Asp Ala Leu Asn Phe Leu Pro Asp His Pro Ala
165 170 175
cgt aca ctg cag gac acc ttc cac att gcg ccg gaa ggc tcc cgc cag 576
Arg Thr Leu Gln Asp Thr Phe His Ile Ala Pro Glu Gly Ser Arg Gln
180 185 190
gtg ctg cgt acc cac acc tca cca gtt cag gtg cgc acc atg ctc aac 624
Val Leu Arg Thr His Thr Ser Pro Val Gln Val Arg Thr Met Leu Asn
195 200 205
cgt gaa gtt ccg atc tac atc gca tgc cct ggc cgt gtg ttc cgt acc 672
Arg Glu Val Pro Ile Tyr Ile Ala Cys Pro Gly Arg Val Phe Arg Thr
210 215 220
gac gaa ctc gat gca aca cac acc cct gtg ttc cac cag atc gaa ggc 720
Asp Glu Leu Asp Ala Thr His Thr Pro Val Phe His Gln Ile Glu Gly
225 230 235 240
ctt gca gtt gat aag ggt ctg act atg gct cac ctg cgc ggc acc ttg 768
Leu Ala Val Asp Lys Gly Leu Thr Met Ala His Leu Arg Gly Thr Leu
245 250 255
gat cac ctg gcc aag gaa ctc ttc ggc cca gag acc aag act cgt atg 816
Asp His Leu Ala Lys Glu Leu Phe Gly Pro Glu Thr Lys Thr Arg Met
260 265 270
cgc tcc aac tac ttc cca ttc acc gag cct tcc gct gag gtt gat gtg 864
Arg Ser Asn Tyr Phe Pro Phe Thr Glu Pro Ser Ala Glu Val Asp Val
275 280 285
tgg ttc cct aac aag aag ggt ggt gca ggc tgg atc gag tgg ggt ggc 912
Trp Phe Pro Asn Lys Lys Gly Gly Ala Gly Trp Ile Glu Trp Gly Gly
290 295 300
tgt ggc atg gtc aac cca aac gtg ctt cgt gcc gtg ggt gtt gac cca 960
Cys Gly Met Val Asn Pro Asn Val Leu Arg Ala Val Gly Val Asp Pro
305 310 315 320
gag gag tac acc gga ttc gcg ttc ggc atg ggc att gaa cgt acc ctg 1008
Glu Glu Tyr Thr Gly Phe Ala Phe Gly Met Gly Ile Glu Arg Thr Leu
325 330 335
cag ttc cgc aac ggc ctg agc gat atg cgc gac atg gtt gag ggc gat 1056
Gln Phe Arg Asn Gly Leu Ser Asp Met Arg Asp Met Val Glu Gly Asp
340 345 350
att cgc ttc acc ctc cca ttc ggc att cag gca 1089
Ile Arg Phe Thr Lau Pro Phe Gly Ile Gln Ala
355 360
<210>92
<211>363
<212>PRT
<213>谷氨酸棒状杆菌
<400>92
Met Ala Gly Leu Val Asp Leu Thr Ile Asn Met Arg Glu Gly Leu Asp
1 5 10 15
Tyr Thr Val Ser Glu Ile Gln Leu Thr Glu Ala Ser Leu Asn Glu Ala
20 25 30
Ala Asp Ala Ala Ile Lys Ala Phe Asp Gly Ala Gln Asn Leu Asp Glu
35 40 45
Leu Ala Ala Leu Arg Arg Asp His Leu Gly Asp Ala Ala Pro Ile Pro
50 55 60
Gln Ala Arg Arg Ser Leu Gly Thr Ile Pro Lys Asp Gln Arg Lys Asp
65 70 75 80
Ala Gly Arg Phe Val Asn Met Ala Leu Gly Arg Ala Glu Lys His Phe
85 90 95
Ala Gln Val Lys Val Val Leu Glu Glu Lys Arg Asn Ala Glu Val Leu
100 105 110
Glu Leu Glu Arg Val Asp Val Thr Val Pro Thr Thr Arg Glu Gln Val
115 120 125
Gly Ala Leu His Pro Ile Thr Ile Leu Asn Glu Gln Ile Ala Asp Ile
130 135 140
Phe Val Gly Met Gly Trp Glu Ile Ala Glu Gly Pro Glu Val Glu Ala
145 150 155 160
Glu Tyr Phe Asn Phe Asp Ala Leu Asn Phe Leu Pro Asp His Pro Ala
165 170 175
Arg Thr Leu Gln Asp Thr Phe His Ile Ala Pro Glu Gly Ser Arg Gln
180 185 190
Val Leu Arg Thr His Thr Ser Pro Val Gln Val Arg Thr Met Leu Asn
195 200 205
Arg Glu Val Pro Ile Tyr Ile Ala Cys Pro Gly Arg Val Phe Arg Thr
210 215 220
Asp Glu Leu Asp Ala Thr His Thr Pro Val Phe His Gln Ile Glu Gly
225 230 235 240
Leu Ala Val Asp Lys Gly Leu Thr Met Ala His Leu Arg Gly Thr Leu
245 250 255
Asp His Leu Ala Lys Glu Leu Phe Gly Pro Glu Thr Lys Thr Arg Met
260 265 270
Arg Ser Asn Tyr Phe Pro Phe Thr Glu Pro Ser Ala Glu Val Asp Val
275 280 285
Trp Phe Pro Asn Lys Lys Gly Gly Ala Gly Trp Ile Glu Trp Gly Gly
290 295 300
Cys Gly Met Val Asn Pro Asn Val Leu Arg Ala Val Gly Val Asp Pro
305 310 315 320
Glu Glu Tyr Thr Gly Phe Ala Phe Gly Met Gly Ile Glu Arg Thr Leu
325 330 335
Gln Phe Arg Asn Gly Leu Ser Asp Met Arg Asp Met Val Glu Gly Asp
340 345 350
Ile Arg Phe Thr Leu Pro Phe Gly Ile Gln Ala
355 360
<210>93
<211>1857
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(1857)
<223>RXA07027
<400>93
atg gca ttt tta cac ttt ggc tca gaa ctg aaa cga ttt ggc cgc gga 48
Met Ala Phe Leu His Phe Gly Ser Glu Leu Lys Arg Phe Gly Arg Gly
1 5 10 15
aaa ctc cca cca ttg ggg ttt gtt gtg gtc atg ttg ctg ccg ttg ctt 96
Lys Leu Pro Pro Leu Gly Phe Val Val Val Met Leu Leu Pro Leu Leu
20 25 30
ttc ggc ggc gtc ttt gtt tct gcc tac tat gac ccc atc ggt ggc ttg 144
Phe Gly Gly Val Phe Val Ser Ala Tyr Tyr Asp Pro Ile Gly Gly Leu
35 40 45
tcc aag ctt ccc gtg gcc gtg gtc aat cag gac gaa ggt gaa ctc gat 192
Ser Lys Leu Pro Val Ala Val Val Asn Gln Asp Glu Gly Glu Leu Asp
50 55 60
gcc ggc gcc cag gtg gtg gag aac ctc ctg gag caa gac gac atc aaa 240
Ala Gly Ala Gln Val Val Glu Asn Leu Leu Glu Gln Asp Asp Ile Lys
65 70 75 80
ttc att gaa gtc agc gcc gag gaa gcc cgc gag gga atc aac gac ggc 288
Phe Ile Glu Val Ser Ala Glu Glu Ala Arg Glu Gly Ile Asn Asp Gly
85 90 95
acc tac tac ttc ggc att gaa att ccg aag aac ttc agc gat tct att 336
Thr Tyr Tyr Phe Gly Ile Glu Ile Pro Lys Asn Phe Ser Asp Ser Ile
100 105 110
gcc agc gtg acc agc gat tca ccc gcg cca gca acc gtc aac gcg gta 384
Ala Ser Val Thr Ser Asp Ser Pro Ala Pro Ala Thr Val Asn Ala Val
115 120 125
ttc aac aac agc aac ggc ttc att gcc tcc atg ctg ggc aac cag gtg 432
Phe Asn Asn Ser Asn Gly Phe Ile Ala Ser Met Leu Gly Asn Gln Val
130 135 140
gtc aac act gtt gtg gag acc atg gac acg gaa ttc ggc gtc cgc att 480
Val Asn Thr Val Val Glu Thr Met Asp Thr Glu Phe Gly Val Arg Ile
145 150 155 160
gtg gat aac atg ctc gtc ggt ttc tcc acc ttg ggc gac ggc atg aac 528
Val Asp Asn Met Leu Val Gly Phe Ser Thr Leu Gly Asp Gly Met Asn
165 170 175
caa gcc gcc gaa ggt gcc act acg ctc agc gat ggc gtc ggt tcc gcc 576
Gln Ala Ala Glu Gly Ala Thr Thr Leu Ser Asp Gly Val Gly Ser Ala
180 185 190
aac gac ggt gca gtt cag ctt gcc gac ggc gcg gtc acc ctg cgc gac 624
Asn Asp Gly Ala Val Gln Leu Ala Asp Gly Ala Val Thr Leu Arg Asp
195 200 205
ggc atc gca agt gcc aat gag ggt gcg caa tcg ctt gcc gac ggc gcc 672
Gly Ile Ala Ser Ala Asn Glu Gly Ala Gln Ser Leu Ala Asp Gly Ala
210 215 220
agc cag ctc gac acc ggc ctc ggc tcc gcg gct aca ggc agc caa acg 720
Ser Gln Leu Asp Thr Gly Leu Gly Ser Ala Ala Thr Gly Ser Gln Thr
225 230 235 240
ctc gcc gac ggt cta tcc agc ctg tct gcg ggc acc gcc caa cta ggc 768
Leu Ala Asp Gly Leu Ser Ser Leu Ser Ala Gly Thr Ala Gln Leu Gly
245 250 255
caa ggc gca acc cag gtt tca gat ggc gtg ggc caa ctt gtc gac caa 816
Gln Gly Ala Thr Gln Val Ser Asp Gly Val Gly Gln Leu Val Asp Gln
260 265 270
gta gca cca ctg acc gcc tat gtt cca gac atc aac tct cag ttg atc 864
Val Ala Pro Leu Thr Ala Tyr Val Pro Asp Ile Asn Ser Gln Leu Ile
275 280 285
acc ctg cgc gac ggc gca gcc acc att gcc tct gaa cta tct gat ccc 912
Thr Leu Arg Asp Gly Ala Ala Thr Ile Ala Ser Glu Leu Ser Asp Pro
290 295 300
tcc agc acc tac cgc tcc ggc gtg gac tcc gct gtg agc gca tcc cag 960
Ser Ser Thr Tyr Arg Ser Gly Val Asp Ser Ala Val Ser Ala Ser Gln
305 310 315 320
caa cta gca gcc ggc ctg caa acc ctg aaa gac gga tcc agc caa ctc 1008
Gln Leu Ala Ala Gly Leu Gln Thr Leu Lys Asp Gly Ser Ser Gln Leu
325 330 335
agc atc ggt gca cgc acc ctc gct gat ggc acc agc caa ttg gcc gca 1056
Ser Ile Gly Ala Arg Thr Leu Ala Asp Gly Thr Ser Gln Leu Ala Ala
340 345 350
ggt tcc gaa cag cta gtt gtt ggc gca caa gca ctg cgc gac ggc acc 1104
Gly Ser Glu Gln Leu Val Val Gly Ala Gln Ala Leu Arg Asp Gly Thr
355 360 365
gtc cag ctt gat gaa ggc tcc agc gaa ctc gcc ctc aaa ctc acc gac 1152
Val Gln Leu Asp Glu Gly Ser Ser Glu Leu Ala Leu Lys Leu Thr Asp
370 375 380
ggc gca agc caa gta cca acc ttc gct gac ggc gca gac acc acc atc 1200
Gly Ala Ser Gln Val Pro Thr Phe Ala Asp Gly Ala Asp Thr Thr Ile
385 390 395 400
gca acc cca gtt gaa aca gaa caa gca gga gac acc aca ccg ctc ttc 1248
Ala Thr Pro Val Glu Thr Glu Gln Ala Gly Asp Thr Thr Pro Leu Phe
405 410 415
ggt att ggt ctc gca cca ttc ttc atg gct gtc ggc ctg ttc atg gga 1296
Gly Ile Gly Leu Ala Pro Phe Phe Met Ala Val Gly Leu Phe Met Gly
420 425 430
gca acc gtt gcc tgg atg atc ctg cac cca atc agt cgc cgc gca ctc 1344
Ala Thr Val Ala Trp Met Ile Leu His Pro Ile Ser Arg Arg Ala Leu
435 440 445
gac tcc cgc atg gga ggc ttc cga ggc acc ctg gca agc tac ctt cca 1392
Asp Ser Arg Met Gly Gly Phe Arg Gly Thr Leu Ala Ser Tyr Leu Pro
450 455 460
tca aca gtc tta ggc ctt ggc caa gca acc atc atg tgg gca gta ctg 1440
Ser Thr Val Leu Gly Leu Gly Gln Ala Thr Ile Met Trp Ala Val Leu
465 470 475 480
tac ttc ctg ctc gac ctc aat cca gct cac cca gct gga ctg tgg atg 1488
Tyr Phe Leu Leu Asp Leu Asn Pro Ala His Pro Ala Gly Leu Trp Met
485 490 495
gcg atg gtc gcc atc tca tgg gta ttc atc tcc att acc cat atg ttc 1536
Ala Met Val Ala Ile Ser Trp Val Phe Ile Ser Ile Thr His Met Phe
500 505 510
aac aac gtg gca gga ccc tcc gca ggc cgt gtg ctg tcc atc gtg atg 1584
Asn Asn Val Ala Gly Pro Ser Ala Gly Arg Val Leu Ser Ile Val Met
515 520 525
atg tcc ttc cag cta gtc tcc tcc ggt ggc cta tac cca cca gaa acc 1632
Met Ser Phe Gln Leu Val Ser Ser Gly Gly Leu Tyr Pro Pro Glu Thr
530 535 540
cag cca gca ttc ttc cac tgg ttc cac acc tac gac ccg atc acc tac 1680
Gln Pro Ala Phe Phe His Trp Phe His Thr Tyr Asp Pro Ile Thr Tyr
545 550 555 560
gca gtc aac ctc gtg cgc caa atg atc ttc aac gaa acc cca tcc aac 1728
Ala Val Asn Leu Val Arg Gln Met Ile Phe Asn Glu Thr Pro Ser Asn
565 570 575
gac cca cgc ttc ata caa gca atc tgg gta ctg ctc ttc atc tgg gca 1776
Asp Pro Arg Phe Ile Gln Ala Ile Trp Val Leu Leu Phe Ile Trp Ala
580 585 590
ctg atg ctc gcc atc tcc acc ctg gcg aat aga aca aac aag gtt ctt 1824
Leu Met Leu Ala Ile Ser Thr Leu Ala Asn Arg Thr Asn Lys Val Leu
595 600 605
cgc atg aag gac tac cac cca gaa ctg aag gtc 1857
Arg Met Lys Asp Tyr His Pro Glu Leu Lys Val
610 615
<210>94
<211>619
<212>PRT
<213>谷氨酸棒状杆菌
<400>94
Met Ala Phe Leu His Phe Gly Ser Glu Leu Lys Arg Phe Gly Arg Gly
1 5 10 15
Lys Leu Pro Pro Leu Gly Phe Val Val Val Met Leu Leu Pro Leu Leu
20 25 30
Phe Gly Gly Val Phe Val Ser Ala Tyr Tyr Asp Pro Ile Gly Gly Leu
35 40 45
Ser Lys Leu Pro Val Ala Val Val Asn Gln Asp Glu Gly Glu Leu Asp
50 55 60
Ala Gly Ala Gln Val Val Glu Asn Leu Leu Glu Gln Asp Asp Ile Lys
65 70 75 80
Phe Ile Glu Val Ser Ala Glu Glu Ala Arg Glu Gly Ile Asn Asp Gly
85 90 95
Thr Tyr Tyr Phe Gly Ile Glu Ile Pro Lys Asn Phe Ser Asp Ser Ile
100 105 110
Ala Ser Val Thr Ser Asp Ser Pro Ala Pro Ala Thr Val Asn Ala Val
115 120 125
Phe Asn Asn Ser Asn Gly Phe Ile Ala Ser Met Leu Gly Asn Gln Val
130 135 140
Val Asn Thr Val Val Glu Thr Met Asp Thr Glu Phe Gly Val Arg Ile
145 150 155 160
Val Asp Asn Met Leu Val Gly Phe Ser Thr Leu Gly Asp Gly Met Asn
165 170 175
Gln Ala Ala Glu Gly Ala Thr Thr Leu Ser Asp Gly Val Gly Ser Ala
180 185 190
Asn Asp Gly Ala Val Gln Leu Ala Asp Gly Ala Val Thr Leu Arg Asp
195 200 205
Gly Ile Ala Ser Ala Asn Glu Gly Ala Gln Ser Leu Ala Asp Gly Ala
210 215 220
Ser Gln Leu Asp Thr Gly Leu Gly Ser Ala Ala Thr Gly Ser Gln Thr
225 230 235 240
Leu Ala Asp Gly Leu Ser Ser Leu Ser Ala Gly Thr Ala Gln Leu Gly
245 250 255
Gln Gly Ala Thr Gln Val Ser Asp Gly Val Gly Gln Leu Val Asp Gln
260 265 270
Val Ala Pro Leu Thr Ala Tyr Val Pro Asp Ile Asn Ser Gln Leu Ile
275 280 285
Thr Leu Arg Asp Gly Ala Ala Thr Ile Ala Ser Glu Leu Ser Asp Pro
290 295 300
Ser Ser Thr Tyr Arg Ser Gly Val Asp Ser Ala Val Ser Ala Ser Gln
305 310 315 320
Gln Leu Ala Ala Gly Leu Gln Thr Leu Lys Asp Gly Ser Ser Gln Leu
325 330 335
Ser Ile Gly Ala Arg Thr Leu Ala Asp Gly Thr Ser Gln Leu Ala Ala
340 345 350
Gly Ser Glu Gln Leu Val Val Gly Ala Gln Ala Leu Arg Asp Gly Thr
355 360 365
Val Gln Leu Asp Glu Gly Ser Ser Glu Leu Ala Leu Lys Leu Thr Asp
370 375 380
Gly Ala Ser Gln Val Pro Thr Phe Ala Asp Gly Ala Asp Thr Thr Ile
385 390 395 400
Ala Thr Pro Val Glu Thr Glu Gln Ala Gly Asp Thr Thr Pro Leu Phe
405 410 415
Gly Ile Gly Leu Ala Pro Phe Phe Met Ala Val Gly Leu Phe Met Gly
420 425 430
Ala Thr Val Ala Trp Met Ile Leu His Pro Ile Ser Arg Arg Ala Leu
435 440 445
Asp Ser Arg Met Gly Gly Phe Arg Gly Thr Leu Ala Ser Tyr Leu Pro
450 455 460
Ser Thr Val Leu Gly Leu Gly Gln Ala Thr Ile Met Trp Ala Val Leu
465 470 475 480
Tyr Phe Leu Leu Asp Leu Asn Pro Ala His Pro Ala Gly Leu Trp Met
485 490 495
Ala Met Val Ala Ile Ser Trp Val Phe Ile Ser Ile Thr His Met Phe
500 505 510
Asn Asn Val Ala Gly Pro Ser Ala Gly Arg Val Leu Ser Ile Val Met
515 520 525
Met Ser Phe Gln Leu Val Ser Ser Gly Gly Leu Tyr Pro Pro Glu Thr
530 535 540
Gln Pro Ala Phe Phe His Trp Phe His Thr Tyr Asp Pro Ile Thr Tyr
545 550 555 560
Ala Val Asn Leu Val Arg Gln Met Ile Phe Asn Glu Thr Pro Ser Asn
565 570 575
Asp Pro Arg Phe Ile Gln Ala Ile Trp Val Leu Leu Phe Ile Trp Ala
580 585 590
Leu Met Leu Ala Ile Ser Thr Leu Ala Asn Arg Thr Asn Lys Val Leu
595 600 605
Arg Met Lys Asp Tyr His Pro Glu Leu Lys Val
610 615
<210>95
<211>1074
<212>DNA
<213>谷氨酸棒状杆菌
<220>
<221>CDS
<222>(1)..(1074)
<223>RXA07028
<400>95
atg aag ccc acc gtt aat gtt gtg ttc aat gcg cat cac ccc aaa gat 48
Met Lys Pro Thr Val Asn Val Val Phe Asn Ala His His Pro Lys Asp
1 5 10 15
acg cag ccg ttg gat aag ttc ttc gat aaa gaa ctt aaa gac aca cat 96
Thr Gln Pro Leu Asp Lys Phe Phe Asp Lys Glu Leu Lys Asp Thr His
20 25 30
cat ctc gat ata acg gtg ggt tat atc agt gag aaa tca cta caa tat 144
His Leu Asp Ile Thr Val Gly Tyr Ile Ser Glu Lys Ser Leu Gln Tyr
35 40 45
ttg ctt ctt att gca ggc act cac ccc gac ctc acc atc aca ctc acc 192
Leu Leu Leu Ile Ala Gly Thr His Pro Asp Leu Thr Ile Thr Leu Thr
50 55 60
tgt gga atg cac gct cgt gaa ggc atg act gct gcc caa ctg cat cat 240
Cys Gly Met His Ala Arg Glu Gly Met Thr Ala Ala Gln Leu His His
65 70 75 80
gcg cga gtg ctc cat gac tac tta agc gac cat gat cga ggc ggg gtg 288
Ala Arg Val Leu His Asp Tyr Leu Ser Asp His Asp Arg Gly Gly Val
85 90 95
ttc gtt att ccc cga ttg cgt tat cac ggc aaa atc tat ctt ttc cac 336
Phe Val Ile Pro Arg Leu Arg Tyr His Gly Lys Ile Tyr Leu Phe His
100 105 110
aag aac cag cac aca gat cct att gct tat atc ggt agc gct aac ctc 384
Lys Asn Gln His Thr Asp Pro Ile Ala Tyr Ile Gly Ser Ala Asn Leu
115 120 125
tca gcc atc gtt cct ggg tac acc tct aca ttc gag acc ggc gtc atc 432
Ser Ala Ile Val Pro Gly Tyr Thr Ser Thr Phe Glu Thr Gly Val Ile
130 135 140
tta gac ccc gca cct gaa gat ctc gtg ctt cat ctc aac cgt gat gtc 480
Leu Asp Pro Ala Pro Glu Asp Leu Val Leu His Leu Asn Arg Asp Val
145 150 155 160
gta ccc cta tgt gtc ccc att gac acc gcg cat gtc ccc atc att aaa 528
Val Pro Leu Cys Val Pro Ile Asp Thr Ala His Val Pro Ile Ile Lys
165 170 175
gat caa gaa tcc ccg atg aag cac gtc gct gaa gca aca gct gtg tcc 576
Asp Gln Glu Ser Pro Met Lys His Val Ala Glu Ala Thr Ala Val Ser
180 185 190
acc tct gat gtt gtt gcc atc atg tcc agc cca ttt act tat agt ttt 624
Thr Ser Asp Val Val Ala Ile Met Ser Ser Pro Phe Thr Tyr Ser Phe
195 200 205
gac ctt aaa ctc aaa gcc act gcc agc agc aac ctc aat gct cat aac 672
Asp Leu Lys Leu Lys Ala Thr Ala Ser Ser Asn Leu Asn Ala His Asn
210 215 220
tca ggc ggt ggc gcg cgc aaa cag aaa aac ggt agc ttc ctt gca cgc 720
Ser Gly Gly Gly Ala Arg Lys Gln Lys Asn Gly Ser Phe Leu Ala Arg
225 230 235 240
aat tgg tat gag ggc gaa atc att gtc ggt gtc gag aca aca aga ctc 768
Asn Trp Tyr Glu Gly Glu Ile Ile Val Gly Val Glu Thr Thr Arg Leu
245 250 255
cca ggt tac cca caa aac aaa tcc gaa ttc act gcg gtc act gat gac 816
Pro Gly Tyr Pro Gln Asn Lys Ser Glu Phe Thr Ala Val Thr Asp Asp
260 265 270
ggc tgg tca ttt gtt tgc aaa atc agc gga gga aac gga aag aac cta 864
Gly Trp Ser Phe Val Cys Lys Ile Ser Gly Gly Asn Gly Lys Asn Leu
275 280 285
cgc agc aaa ggt gac ctg tcc atc ctc ggt acg tgg tta aag tct cga 912
Arg Ser Lys Gly Asp Leu Ser Ile Leu Gly Thr Trp Leu Lys Ser Arg
290 295 300
ttc att gaa caa ggt gcc ctg gaa tac ggc gag gat gcc acc caa gaa 960
Phe Ile Glu Gln Gly Ala Leu Glu Tyr Gly Glu Asp Ala Thr Gln Glu
305 310 315 320
aac atc gac cgt ttt ggg aga aca cat atg acc atg cgc tat cac cca 1008
Asn Ile Asp Arg Phe Gly Arg Thr His Met Thr Met Arg Tyr His Pro
325 330 335
gat ttc gat gtg tgg tca ttc gat ctc agc caa acc ccg aag cct tcg 1056
Asp Phe Asp Val Trp Ser Phe Asp Leu Ser Gln Thr Pro Lys Pro Ser
340 345 350
aca cag att ggg cag gat 1074
Thr Gln Ile Gly Gln Asp
355
<210>96
<211>358
<212>PRT
<213>谷氨酸棒状杆菌
<400>96
Met Lys Pro Thr Val Asn Val Val Phe Asn Ala His His Pro Lys Asp
1 5 10 15
Thr Gln Pro Leu Asp Lys Phe Phe Asp Lys Glu Leu Lys Asp Thr His
20 25 30
His Leu Asp Ile Thr Val Gly Tyr Ile Ser Glu Lys Ser Leu Gln Tyr
35 40 45
Leu Leu Leu Ile Ala Gly Thr His Pro Asp Leu Thr Ile Thr Leu Thr
50 55 60
Cys Gly Met His Ala Arg Glu Gly Met Thr Ala Ala Gln Leu His His
65 70 75 80
Ala Arg Val Leu His Asp Tyr Leu Ser Asp His Asp Arg Gly Gly Val
85 90 95
Phe Val Ile Pro Arg Leu Arg Tyr His Gly Lys Ile Tyr Leu Phe His
100 105 110
Lys Asn Gln His Thr Asp Pro Ile Ala Tyr Ile Gly Ser Ala Asn Leu
115 120 125
Ser Ala Ile Val Pro Gly Tyr Thr Ser Thr Phe Glu Thr Gly Val Ile
130 135 140
Leu Asp Pro Ala Pro Glu Asp Leu Val Leu His Leu Asn Arg Asp Val
145 150 155 160
Val Pro Leu Cys Val Pro Ile Asp Thr Ala His Val Pro Ile Ile Lys
165 170 175
Asp Gln Glu Ser Pro Met Lys His Val Ala Glu Ala Thr Ala Val Ser
180 185 190
Thr Ser Asp Val Val Ala Ile Met Ser Ser Pro Phe Thr Tyr Ser Phe
195 200 205
Asp Leu Lys Leu Lys Ala Thr Ala Ser Ser Asn Leu Asn Ala His Asn
210 215 220
Ser Gly Gly Gly Ala Arg Lys Gln Lys Asn Gly Ser Phe Leu Ala Arg
225 230 235 240
Asn Trp Tyr Glu Gly Glu Ile Ile Val Gly Val Glu Thr Thr Arg Leu
245 250 255
Pro Gly Tyr Pro Gln Asn Lys Ser Glu Phe Thr Ala Val Thr Asp Asp
260 265 270
Gly Trp Ser Phe Val Cys Lys Ile Ser Gly Gly Asn Gly Lys Asn Leu
275 280 285
Arg Ser Lys Gly Asp Leu Ser Ile Leu Gly Thr Trp Leu Lys Ser Arg
290 295 300
Phe Ile Glu Gln Gly Ala Leu Glu Tyr Gly Glu Asp Ala Thr Gln Glu
305 310 315 320
Asn Ile Asp Arg Phe Gly Arg Thr His Met Thr Met Arg Tyr His Pro
325 330 335
Asp Phe Asp Val Trp Ser Phe Asp Leu Ser Gln Thr Pro Lys Pro Ser
340 345 350
Thr Gln Ile Gly Gln Asp
355