表皮葡萄球菌调节信号多肽及编码的DNA 【技术领域】
本发明属生物技术领域，具体涉及作为表皮葡萄球菌信号传递系统关键新蛋白：组氨酸蛋白激酶和反应调节蛋白，以及利用该类蛋白寻找抑菌药物的方法。更具体地，本发明涉及编码该类多肽的DNA；制备该类多肽的方法，包括基因克隆、基因表达、蛋白纯化和功能测定；制备该类多肽的抗体；以及利用该类多肽筛选抑菌活性化合物。
背景技术
葡萄球菌与医院内化脓性感染相关，尤以金黄色葡萄球菌和表皮葡萄球菌最为密切，表皮葡萄球菌所致感染与一些高分子医疗器材在体内的留置有关，如静脉导管、起搏器、脑脊液引流装置、人工器官等。随着上述医疗材料的使用日益频繁，此类院内感染问题日益严峻，且易发展为慢性感染，对抗生素的抗性较高。
已知在各种原核生物中普遍存在双组分调控系统，为原核细胞所特有的信号系统，参与调控生物生长、毒力等多种生物学功能。通常由一对相应的蛋白组成，称为组氨酸蛋白激酶和反应调节蛋白。组氨酸蛋白激酶为跨膜蛋白，当外界信号作用于组氨酸蛋白激酶的膜外区域，产生信号，使该蛋白发生自身磷酸化。随后，磷酸化的组氨酸蛋白激酶作用于相应的反应调节蛋白发挥调控作用。不同的双组分蛋白，调控细菌的不同生物学活性。然而，由于研究手段的局限性，对表皮葡萄球菌双组分调控系统及对生物学功能地调控缺乏系统地了解和实际应用。
【发明内容】
本发明的目的是提供一组表皮葡萄球菌信号传递系统的新蛋白，包括组氨酸蛋白激酶和反应调节蛋白。本发明的另一目的是提供制备该类多肽的方法和编码该类多肽的DNA，及其制备该类多肽的抗体，本发明的进一步目的是利用该类多肽筛选药物化合物。
本发明通过对表葡菌ATCC12228株的全基因组测序和序列分析，采用序列同源性比较和功能域分析等生物信息学方法确定了16对表皮葡萄球菌的双组分调控信号蛋白(包括组氨酸蛋白激酶和反应调节蛋白)，并制备了相应的蛋白，经对上述蛋白功能域空间结构的模建和实验证实该类蛋白存在保守性结构和活性位点，可作为筛选药物的“靶点”。
本发明表皮葡萄球菌组氨酸蛋白激酶和反应调节蛋白中包括16对双组分调控蛋白，其中16个组氨酸蛋白激酶均具有结合ATP的激酶活性功能域(HATPase_c功能域)，分别具有SEQ ID No.1-16的序列结构。其中16个反应调节蛋白均存在接受磷酸基团的功能域(REC功能域)，分别具有SEQ ID No.17-32的序列结构。
本发明采用PMODELIHG软件对上述相对保守的功能域片段进行同源模建，结果显示：组氨酸蛋白激酶HATPase_c功能域的结构特征是在AMP-PNP的结合部位形成袋状结构，且在其周围有保守的基序围绕，所述AMP-PNP是一种ATP类似物；反应调节蛋白的REC功能域模拟结构由5个α螺旋和5个β折叠组成，其中存在三个天冬氨酸残基构成接受磷酸基团的活性位点，且接受磷酸基团的过程需要二价阳离子(Mg2+或Mn2+)的辅助，所述天冬氨酸残基是D7、D8、D51。
本发明所述的表皮葡萄球菌组氨酸蛋白激酶具有SEQ ID No.1-16的氨基酸序列结构，由SEQ ID No.33-48的DNA核苷酸序列编码；所述的反应调节蛋白具有SEQ ID No.17-32的氨基酸序列结构，由SEQ ID No.49-64的DNA核苷酸序列编码。
本发明涉及由SEQ ID No.1-32构成的蛋白的氨基酸序列的同源序列及片断序列，和SEQ ID No.33-64编码相关同源序列和片断有关多肽的DNA核苷酸序列。所述同源DNA序列是指编码SEQ ID No.33-64所示核苷酸序列或片断，或DNA片断长度在60个连续核苷酸区域内，一般至少70％同源于SEQ ID 1-32，可与之选择性杂交的片断核苷酸序列。所述SEQ ID 1-32的多肽同源序列至少在30个的连续氨基酸序列区域，至少60％以上的同源。
本发明的多肽包括组氨酸蛋白激酶和反应调节蛋白氨基酸序列的功能域部分序列，如具有生物学活性的氨基酸基本序列的多肽片断；或其中部分氨基酸被性质相似的氨基酸取代的多肽；或其氨基酸的一部分中加入或插入其他氨基酸的多肽，以及DNA的核苷酸序列改变，但不影响氨基酸序列的多肽，如同种氨基酸使用不同密码子。
本发明提供了制备表皮葡萄球菌组氨酸蛋白激酶和反应调节蛋白(SEQ IDNo.1-32)方法，包括制备仅含生物学活性功能区的编码序列的DNA片断，克隆至适合的表达载体，转染宿主菌，在适于的条件下培养该宿主细胞，以及从培养物中回收和纯化所需蛋白。
上述技术方案中所有基本分子生物学操作均参照《分子克隆实验指南》。
本发明将编码相应蛋白的DNA序列与表达载体重组，形成重组表达质粒。本发明不限于特定的表达质粒。在一优选实施方案中，本发明使用原核表达载体，例如pet28a等。
上述重组表达载体可按常规方法导入适宜宿主细菌。本发明不局限于任何特定的宿主细胞，只要它能够表达所述重组表达载体。在一优选实施方案中，本发明使用大肠杆菌JM109等。
本发明的多肽包括磷酸化反应产物。
本发明还提供抗本发明多肽的多克隆抗体或单克隆抗体，使用本发明的多肽或其片断作为免疫原，按常规方法制备特异性抗体。可用于细菌细胞内的多肽表达量，也可用于研究所述多肽与细菌致病的关系，或用于阻断细菌细胞之间的信号转递。
可以将本发明DNA的反义核酸的DNA插入合适的载体中，产生反义RNA；也可用合成方法产生反义RNA。反义RNA可用于控制细菌细胞内多肽水平。
本发明多肽功能域结构可用于筛选活性化合物，能阻断组氨酸蛋白激酶或反应调节蛋白质间的磷酸基团转移和蛋白质的磷酸化，抑制细菌细胞内或细胞之间的信号传递，从而抑制葡萄球菌的生长。
本发明在表皮葡萄球菌ATCC12228株中确定了16对完整的双组分调控系统的基因核苷酸序列及其功能。经实验证实了组氨酸蛋白激酶和反应调节蛋白的磷酸化或两者分子之间的磷酸基团转移可调控葡萄球菌的生物学活性，抑制组氨酸蛋白激酶和反应调节蛋白的磷酸化或磷酸基团的转移可抑制细菌的生长。对其保守性功能域的空间结构模建确定了存在保守结构和活性位点可作为筛选活性化合物的药物“靶点”。
附图说明：
图1是表皮葡萄球菌双组分调控蛋白保守功能域空间结构同源模建图。其中，1a是组氨酸蛋白激酶HATPase_c功能域模拟结构，1b是反应调节蛋白REC功能域模拟结构。
【具体实施方式】
实施例1 确定16对表皮葡萄球菌双组分调控系统
运用序列同源性比较，功能域分析等生物信息学方法，在表皮葡萄球菌ATCC12228株全基因组(GeneBank收录号AE015929)中确定了16对双组分调控基因。表1列出了上述双组分调控基因的基本情况，包括基因长度等，以及氨基酸序列同源性比较结果(blastp)。
表1组氨酸蛋白激酶开放阅读框编号 氨基酸序列长度同源性最高的细菌氨基酸序列同源性se0019 610金黄色葡萄球菌531/610(87％)se0121 363破伤风梭菌86/305(28％)se0166 512金黄色葡萄球菌354/511(69％)se0428 346金黄色葡萄球菌220/346(63％)se0478 321金黄色葡萄球菌239/316(75％)se1000 243金黄色葡萄球菌119/235(50％)se1099 456金黄色葡萄球菌286/449(63％)se1175 589金黄色葡萄球菌359/584(61％)se1368 565金黄色葡萄球菌338/564(59％)se1530 377金黄色葡萄球菌245/370(66％)se1570 348金黄色葡萄球菌300/344(87％)se1637 429金黄色葡萄球菌229/413(55％)se1942 451金黄色葡萄球菌253/452(55％)se1970 344金黄色葡萄球菌238/344(69％)se2011 591金黄色葡萄球菌343/566(60％)se2194 298金黄色葡萄球菌159/293(54％)反应调节蛋白开放阅读框编号 氨基酸序列长度同源性最高的细菌氨基酸序列同源性se0018 233金黄色葡萄球菌227/233(97％)se0120 222丙酮丁醇梭菌78/230(33％)se0165 251金黄色葡萄球菌201/252(79％)se0427 224金黄色葡萄球菌203/224(90％)se0479 229金黄色葡萄球菌179/229(78％)se1002 200金黄色葡萄球菌138/200(69％)se1100 219金黄色葡萄球菌178/219(81％)se1176 241金黄色葡萄球菌218/241(90％)se1369 236金黄色葡萄球菌197/235(83％)se1529 207金黄色葡萄球菌166/207(80％)se1569 209金黄色葡萄球菌186/209(88％)se1638 238金黄色葡萄球菌209/238(87％)se1941 224金黄色葡萄球菌158/224(70％)se1969 218金黄色葡萄球菌191/218(87％)se2012 252金黄色葡萄球菌136/251(54％)se2195 221金黄色葡萄球菌175/221(79％)
通过与SMART氨基酸序列及功能域数据库进行同源性比较，对16对双组分调控基因中存在的保守性功能域进行分析，结果表明16个组氨酸蛋白激酶中均存在结合ATP的激酶活性功能域(HATPase_c功能域)，16个反应调节蛋白(SEQID No.17-32)均存在接受磷酸基团的功能域(REC功能域)，表2是表皮葡萄球菌双组分调控基因功能域分析结果。
表2组氨酸激酶的功能域名*HAMP PAS GAF HisKA HATPase_c功能域计数5 3 2 9 16反应调节蛋白的功能域名*HTH-ARAC HTH-LUXR Trans_regC LytTR REC功能域计数1 4 9 2 16
其中功能域名称均来源于SMART氨基酸序列数据库。
实施例2 对HATPase_c功能域和REC功能域进行空间结构模建
根据上述确定的HATPase_c功能域和REC功能域在双组分调控系统中的相对保守性，运用PMODELING软件对两个功能域进行空间结构的同源模建。结果显示HATPase_c功能域模拟结构，与已报道的大肠杆菌组氨酸蛋白激酶基因EnvZ的HATPase_c功能域的和磁共振(NMR)结构非常相似(Ncbi mmdbId：11091)，即HATPase_c功能域主要结构由4个β折叠和3个α螺旋组成，尤其是在AMP-PNP(一种ATP类似物)的结合部位形成一个袋状结构，且其周围有一些保守的基序(G1 box、G2 box、F box)围绕，见图1a；而REC功能域模拟结构与大肠杆菌的反应调节蛋白基因CheY的REC功能域的和磁共振(NMR)结构也非常相似(NcbimmdbId：3000)，由5个α螺旋和5个β折叠组成，其中存在三个天冬氨酸残基(D7、D8、D51)构成接受磷酸基团的活性位点，且该过程需要二价阳离子(Mg2+或Mn2+)的辅助，见图1b。因此，这两个结构均存在药物设计、筛选的“靶标”，以它们为模板，筛选各类小分子化合物库，能找到一些在空间结构上封闭该活性结构或活性位点的小分子化合物，从而阻断这些双组分调控系统，其作用原理是阻断双组分调控系统信号传导途径的一个或多个环节，包括组氨酸蛋白激酶的自身磷酸化、将磷酸基团从蛋白激酶转移到调节蛋白的转磷酸基过程以及反应调节蛋白的磷酸化等。
实施例3 16对双组分调控基因相关功能的分析
将16对双组分调控基因与已报道的其他革兰阳性菌中有明确功能的双组分基因进行了序列同源性比较，结果发现有8对双组分调控基因与已报道的其他革兰阳性菌中双组分基因有较高的氨基酸序列同源性(同源性大于30％)，表明所述双组分调控系统在同为革兰阳性菌的表皮葡萄球菌中也可能发挥着重要的作用，表3是表皮葡萄球菌双组分蛋白调控的生物学功能同源性比对结果。
表3开放阅读框编号 其他革兰阳性菌 的双组分基因 氨基酸序列 同源性(％)参与调控的功能se0019/se0018 VicK/VicR 46/76调控细菌生长、毒力、外源性DNA摄取se0478/se0479 SaeS/SaeR 78/83调控分泌性蛋白合成se1099/se1100 ArlS/ArlR 69/84调控细菌粘附、自溶、分泌性蛋白活性se1175/se1176 SrrB/SrrA 71/90调控毒力因子表达se1368/se1369 PhoR/PhoP 34/68调控碱性磷酸酶合成se1637/se1638 AgrC/AgrA 60/87调控毒力因子表达se2011/se2012 LytS/LytR 62/56调控细菌自溶se1970/se1969 DegS/DegU 33/40调控蛋白酶生成及外源性DNA摄取
实施例4 双组分基因se1175(SrrB)、se1176(SrrA)原核表达质粒1175-pet28a、1176-pet28a的构建及se1175d(SrrB的HATPase_c功能域片段)原核表达质粒1175d-pet28a的构建。
(1)se1175、se1175d、se1176基因的获取方法如下：
以表葡菌ATCC12228株全基因组DNA为模板，设计相应引物经PCR获得。
se1175引物设计：
正义链 5’＞gcg gat cca tga acc gac taa ata gtg ttg 3’
反义链 5’＞taa cta att tgt ttt gat tta ggt 3’
se1175d引物设计：
正义链 5’＞gcg gat ccc gtg ata tga caa atg aac a 3’
反义链 5’＞taa cta att tgt ttt gat tta ggt 3’
se1176引物设计：
正义链 5’＞gcg gat cca tga cta acg aaa ttt taa tc 3’
反义链 5’＞tat tta gtc ggt tca tca c 3’
单划线代表BamHI内切酶位点，双划线代表XholI内切酶位点，限制性内切酶购自GIBCO公司，大肠杆菌BL21，质粒pet28a Invitrogen公司。按常规分子克隆方法将目的基因或片断重组入质粒pet28a，卡那抗性筛选阳性克隆。
(2)重组质粒1175-pet28a、1176-pet28a、1175d-pet28a的表达及目的蛋白的纯化：
将上述重组质粒1175-pet28a、1176-pet28a、1175d-pet28a按常规方法在IPTG诱导下表达，用SDS-PAGE电泳对目的蛋白进行鉴定，结果显示三种重组质粒分别表达70kd、30kd及30kd左右的蛋白，用申能博彩公司提供的3s NTAHis-Tag Resin层析柱进行蛋白提纯。
实施例5 其余1 5对双组分基因(除外se1175/se1176)原核表达质粒的构建
以表葡菌ATCC12228株全基因组DNA为模板，设计相应引物经PCR获得。
se0019引物设计：
正义链 5’CGG GGA TCC ATG AAG TGG CTT AAA CAA CTA 3’
反义链 5’GGC TTA TTC ATC CCA ATC ACC GTC 3’
se0121引物设计：
正义链 5’CGG GGA TCC ATG GCT AGA GTA GAA AGT TTA 3’
反义链 5’GGC TTA GAC AGG AAT ATT GAA ACT 3’
se0166引物设计：
正义链 5’CGG GGA TCC ATG AAG ACC TAT AAG CCG TAC 3’
反义链 5’GGC CTA GAC AAG TGG TAT TTG GTA 3’
se0428引物设计：
正义链 5’CGG GGA TCC ATG AAT AAT TTT CGA TGG TTT 3’
反义链 5’GGC TTA AAA TGA CAA TCT TGT CAC 3’
se0478引物设计：
正义链 5’CGG GGA TCC ATG TGG TTT AAT GGT CAC ATG 3’
反义链 5’GGC TTA CTT TTT AAA TTG AAA TTT 3’
se1000引物设计：
正义链 5’CGG GGA TCC ATG GAA TCA AGA AGT TGG GGA 3’
反义链 5’GGC TCA AAA GAT TGG TAT CTT CAA 3’
se1099引物设计：
正义链 5’CGG GGA TCC ATG ATT AAG CGC CAA AAA TTA 3’
反义链 5’GGC TTA GTT TAG TAC TGG AAA ACT 3’
se1368引物设计：
正义链 5’CGG GGA TCC ATG TTG AAG TTC CAT TTG CGT 3’
反义链 5’GGC TTA ATT ATC ATT AAA TAG AAC 3’
se1530引物设计：
正义链 5’CGG GGA TCC ATG GAG AAA CCA ACT AGA CTT 3’
反义链 5’GGC TTA TGC ATA AGG ATG CTC CTT 3’
se1570引物设计：
正义链 5’CGG GGA TCC ATG AAT CAC TAC ATA AGA GCT 3’
反义链 5’GGC TTA ATC GCC ACT TGA ATT CTC 3’
se1637引物设计：
正义链 5’CGG GGA TCC ATG GAT GAT ATT AAT CTA TTT 3’
反义链 5’GGC TTA TGA TTC CTT ATT ATT TAT 3’
se1942引物设计：
正义链 5’CGG GGA TCC ATG TTT AAA ACA TTA TAT TCG 3’
反义链 5’GGC CTA AAT AAA TAG TTT TAC AAT 3’
se1970引物设计：
正义链 5’CGG GGA TCC GTG GTA AAT ATG TTG GAG CAA 3’
反义链 5’GGC TTA AAT CGG TAT ATC TAA TGT 3’
se2011引物设计：
正义链 5’CGG GGA TCC ATG AGG ATT GAC GTT ATG CTT 3’
反义链 5’GGCTCA ACG ATT AAC GCT TTC ATT 3’
se2194引物设计：
正义链 5’CGG GGA TCC ATG AAG TTT GCA TAT ATT CAA 3’
反义链 5’GGCTTA TTT TTC TTT AGT AAA ATG 3’
se0018引物设计：
正义链 5’CGG GGA TCC ATG GCT AGA AAA GTT GTT GTA 3’
反义链 5’GGC CTA ATC ATG TTG TTG GAG GAA 3’
se0120引物设计：
正义链 5’CGG GGA TCC GTG ATG TAT AAC ATA ATG ATT 3’
反义链 5’GGC CTA CTC TAG CCA TTT ATA TCC 3’
se0165引物设计：
正义链 5’CGG GGA TCC GTG TTA CCA AAT ACC ACT TGT 3’
反义链 5’GGC CTA TTT TGA TAG CTT ATT ATA 3’
se0427引物设计：
正义链 5’CGG GGA TCC ATG CAA ATA TTA TTA GTC GAA 3’
反义链 5’GGC TTA TTC ATG AGC TAA GTA TCC 3’
se0479引物设计：
正义链 5’CGG GGA TCC GTG AAG ATA AGC ATG ACC CAC 3’
反义链 5’GGC TTA TCG GCT CCT TTC AAA CTT 3’
se1002引物设计：
正义链 5’CGG GGA TCC ATG ATA TCG CTT GTT ATA ACT 3’
反义链 5’GGC TTA AAT CCA CCC CTT TTC ATT 3’
se1100引物设计：
正义链 5’CGG GGA TCC ATG ACA AAT ATT TTA ATT GTA 3’
反义链 5’GGC TCA TCG AAT CAC ATA CCC TAC 3’
se1369引物设计：
正义链 5’CGG GGA TCC ATG TAT AAA AAG ATT AGG AGG 3’
反义链 5’GGC GTC GAC TTA AGC TTT AGG TCT CTC CAG 3’
se1529引物设计：
正义链 5’CGG GGA TCC ATG CAT AAA GTC ATT CTC GTC 3’
反义链 5’GGC TTA AAT CAA TTT ATT TTC CAT 3’
se1569引物设计：
正义链 5’CGG GGA TCC GTG GCG ATT AAA GTT TTA TTT 3’
反义链 5’GGC TTA TTG AAT TAA ATT ATG CTG 3’
se1638引物设计：
正义链 5’CGG GGA TCC ATG AAA ATT TTT GTT TGT GAA 3’
反义链 5’GGC TTA TAT TTT TTT AAC ATT ACG 3’
se1941引物设计：
正义链 5’CGG GGA TCC ATG ATT AAT TGC TTA ATC GTA 3’
反义链 5’GGC TTA AAC ATT TTG GTC CAC CCT 3’
se1969引物设计：
正义链 5’CGG GGA TCC GTG AAA ATA GTT ATA GCG GAT 3’
反义链 5’GGC TTA AAA TTC TAA TAA TTT TTT 3’
se2012 引物设计：
正义链 5’CGG GGA TCC ATG AAA GCG TTA ATC GTT GAT 3’
反义链 5’GGC TTA TTG AAG ACC AAG TTG TTG 3’
se2195引物设计：
正义链 5’CGG GGA TCC ATG AAG ATT TTC ATT GTA GAA 3’
反义链 5’GGC TCA AAC CCT GTA TCC TAC ATT 3’
单划线代表BamHI内切酶位点，双划线代表XholI内切酶位点，波浪线代表SalI内切酶位点，虚线代表EcoRI内切酶位点，限制性内切酶购自GIBCO公司，大肠杆菌BL21，质粒pet28a Invitrogen公司。按常规分子克隆方法将目的基因或片断重组入质粒pet28a，卡那抗性筛选阳性克隆。
SEQUENCE LISTING
Organization Applicant
Street：医学院路138号
City：上海
Country：中国
PostalCode：200032
PhoneNumber：8621-54237524
FaxNumber：8621-54237524
<110>复旦大学
<120>表皮葡萄球菌调节信号多肽及编码的DNA
<160>64
<170>PatentIn version 3.2
<210>1
<211>610
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>1
Met Lys Trp Leu Lys Gln Leu Gln Ser Leu His Thr Lys Leu Val Ile
1 5 10 15
Val Tyr Val Leu Leu Ile Ile Ile Gly Met Gln Ile Ile Gly Leu Tyr
20 25 30
Phe Thr Asn Ser Leu Glu Lys Glu Leu Leu Asp Asn Phe Lys Lys Asn
35 40 45
Ile Thr Gln Tyr Ala Lys Gln Leu Asp Val Ash Ile Glu Lys Val Tyr
50 55 60
Lys Asp Lys Asp Lys Gly Ser Val Asn Ala Gln Lys Asp Ile Gln Asp
65 70 75 80
Leu Leu Asn Glu Tyr Ala Asn Arg Gln Glu Ile Gly Glu Ile Arg Phe
85 90 95
Ile Asp Lys Asp Gln Ile Ile Met Ala Thr Thr Lys Gln Ser Asn Arg
100 105 110
Gly Leu Ile Asn Gln Lys Val Asn Asp Gly Ser Val Gln Lys Ala Leu
115 120 125
Ser Leu Gly Gln Thr Asn Asp His Met Val Leu Lys Asp Tyr Gly Ser
130 135 140
Gly Lys Glu Arg Val Trp Val Tyr Asn Ile Pro Val Lys Val Asp Lys
145 150 155 160
Gln Thr Ile Gly Asp Ile Tyr Ile Glu Ser Lys Ile Asn Asp Val Tyr
165 170 175
Asn Gln Leu Asn Asn Ile Asn Gln Ile Phe Ile Val Gly Thr Ala Ile
180 185 190
Ser Leu Phe Ile Thr Val Ile Leu Gly Phe Phe Ile Ala Arg Thr Ile
195 200 205
Thr Lys Pro Ile Thr Asp Met Arg Asn Gln Thr Val Glu Met Ser Lys
210 215 220
Gly Asn Tyr Thr Gln Arg Val Lys Ile Tyr Gly Asn Asp Glu Ile Gly
225 230 235 240
Glu Leu Ala Leu Ala Phe Asn Asn Leu Ser Lys Arg Val Gln Glu Ala
245 250 255
Gln Ala Asn Thr Glu Ser Glu Lys Arg Arg Leu Asp Ser Val Ile Thr
260 265 270
His Met Ser Asp Gly Ile Leu Ala Thr Asp Arg Arg Gly Arg Val Arg
275 280 285
Ile Ala Asn Asp Met Ala Leu Lys Met Leu Gly Leu Ala Lys Glu Asp
290 295 300
Val Ile Gly Tyr Tyr Met Leu Gly Val Leu Asn Leu Glu Asn Glu Phe
305 310 315 320
Ser Leu Glu Glu Ile Gln Glu Asn Ser Asp Ser Phe Leu Leu Asp Ile
325 330 335
Asn Glu Glu Glu Gly Ile Ile Ala Arg Val Asn Phe Ser Thr Ile Val
340 345 350
Gln Glu Thr Gly Phe Val Thr Gly Tyr Ile Ala Val Leu His Asp Val
355 360 365
Thr Glu Gln Gln Gln Val Glu Arg Glu Arg Arg Glu Phe Val Ala Asn
370 375 380
Val Ser His Glu Leu Arg Thr Pro Leu Thr Ser Met Asn Ser Tyr Ile
385 390 395 400
Glu Ala Leu Glu Glu Gly Ala Trp Gln Asp Lys Glu Leu Ala Pro Ser
405 410 415
Phe Leu Ser Val Thr Arg Glu Glu Thr Glu Arg Met Ile Arg Leu Val
420 425 430
Asn Asp Leu Leu Gln Leu Ser Lys Met Asp Asn Glu Ser Asp Gln Ile
435 440 445
Thr Lys Glu Ile Ile Asp Phe Asn Met Phe Ile Asn Lys Ile Ile Asn
450 455 460
Arg His Glu Met Ala Ala Lys Asp Thr Thr Phe Val Arg Glu Ile Pro
465 470 475 480
Gln Gln Thr Ile Phe Ala Glu Ile Asp Pro Asp Lys Met Thr Gln Val
485 490 495
Phe Asp Asn Val Ile Thr Asn Ala Met Lys Tyr Ser Arg Gly Glu Lys
500 505 510
Arg Val Glu Phe His Val Lys Gln Asn Ala Leu Tyr Asn Arg Met Thr
515 520 525
Ile Arg Ile Lys Asp Asn Gly Ile Gly Ile Pro Ile Asn Lys Val Asp
530 535 540
Lys Ile Phe Asp Arg Phe Tyr Arg Val Asp Lys Ala Arg Thr Arg Lys
545 550 555 560
Met Gly Gly Thr Gly Leu Gly Leu Ala Ile Ser Lys Glu Ile Val Glu
565 570 575
Ala His Asn Gly Arg Ile Trp Ala Asn Ser Val Glu Gly Gln Gly Thr
580 585 590
Ser Ile Phe Ile Thr Leu Pro Cys Glu Ile Ile Glu Asp Gly Asp Trp
595 600 605
Asp Glu
610
<210>2
<211>363
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>2
Met Ala Arg Val Glu Ser Leu Lys Arg Lys Met Thr Arg Leu Ile Ile
1 5 10 15
Ala Ile Ile Val Val Ser Val Gly Leu Val Phe Leu Leu Gln Ile Leu
20 25 30
Leu Asn Val Leu Glu Phe Ser Asn Phe Gly Lys Ser Leu Tyr Thr Asn
35 40 45
Phe Trp Lys Gln Pro His Tyr Ile Phe Ser Phe Thr Ser Ala Cys Ile
50 55 60
Ser Ile Met Thr Pro Leu Leu Ile Ile Tyr Val Phe Thr Gln Phe Phe
65 70 75 80
Tyr Lys Lys Tyr Ile Thr Thr Ser Ile Asn Tyr Leu Asn Asp Thr Ile
85 90 95
Gln Gly Leu Lys Ser Asn Arg Thr Asp Val Glu Tyr Lys Lys Met Ser
100 105 110
Ile Ser Glu Phe Asn Lys Leu Ile Gln Glu Val Glu His Ile His Thr
115 120 125
Tyr Leu Ser Asn Ser Ile Lys Asp Gln Leu Ser Ile Gln Asn Asn Phe
130 135 140
Lys Arg Lys Leu Glu Ile Leu Glu His Asp Ile Lys Thr Pro Leu Thr
145 150 155 160
Val Leu Gln Gly His Ile Glu Leu Leu Arg Lys Ile Asn Arg Ser Asn
165 170 175
Leu Ser Gln Glu Glu Ile His Phe Lys Ile Asn Lys Glu Ala Asp Ile
180 185 190
Ala Leu Gln Ser Ile Asp Arg Ile Asn Asn Gln Leu Gln Ile His Ile
195 200 205
Lys Asn Ile Asn Leu Leu Pro Asn His Ser Asp Thr Ile His Val Asp
210 215 220
Lys Val Ile Lys Ile Ile Lys Glu Ser Tyr Asn Gln Gln Tyr Ile Leu
225 230 235 240
Lys Glu Arg Glu Leu Ile Ile Asp Val Asp His Asp Phe Pro Tyr His
245 250 255
Thr Leu Ser Ile Asn Asn Gln Val Leu Tyr His Val Ile Asp Asn Leu
260 265 270
Ile Asn Asn Ala Leu Lys Tyr Ala Glu Lys Gln Ile Ser Ile Lys Phe
275 280 285
Glu Val Ile Asn Asp His Leu Leu Asn Phe Thr Ile Thr Asn Asp Gly
290 295 300
Arg Ile Phe Thr Glu Asp Glu Leu Asn Ser Ala Lys Glu Trp Gly Thr
305 310 315 320
Lys Gly Lys Glu Ser Asn Gly Ser Gly Ile Gly Leu Tyr Phe Ala Asn
325 330 335
Ile Val Leu Gln Gln Tyr Asn Ser Glu Leu Ile Leu Glu Asn Glu Asn
340 345 350
Lys His Ala Lys Val Ser Phe Asn Ile Pro Val
355 360
<210>3
<211>512
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>3
Met Lys Thr Tyr Lys Pro Tyr Arg His Gln Leu Arg Arg Ser Leu Phe
1 5 10 15
Ala Ser Thr Ile Phe Pro Val Phe Met Val Met Ile Ile Gly Leu Ile
20 25 30
Ser Phe Tyr Ala Ile Tyr Ile Trp Val Glu His Arg Thr Ile His Gln
35 40 45
His Thr Tyr Gln Thr Gln Thr Glu Leu Gln Arg Ile Asp Lys His Phe
50 55 60
His Thr Phe Val Thr Gln Gln Gln Lys Gln Trp Arg His Val Asp Leu
65 70 75 80
Ser His Pro Ala Asp Ile Thr Lys Met Lys Arg Gln Leu Leu Lys Gln
85 90 95
Val His Gln Gln Pro Ala Ile Leu Tyr Tyr Asp Leu Lys Gly Ser Ser
100 105 110
Gln Ser Phe Thr Asn Asn Tyr Glu Gln Leu Asp Thr Thr Lys Met Tyr
115 120 125
Leu Ile Ser Lys Tyr Arg Ile Asp Phe Lys Asp Asp Thr Tyr Ile Leu
130 135 140
Lys Ile Tyr Met Ser Ser Thr Pro Leu Leu Lys Asn Ile Lys Lys Asn
145 150 155 160
Ser Gly Gln Ser Ala Leu Ile Val Asp Ser Tyr Asp Thr Val Leu Tyr
165 170 175
Thr Asn Asp Asp Arg Phe Ser Ile Gly Gln Lys Tyr Gln Pro Pro Gln
180 185 190
Phe Gly Phe Met Asn Glu Ser Leu Lys Leu Asn Ser His His Ala His
195 200 205
Leu Ile Ile Tyr Lys Asp Ile His Glu Thr Ile Glu Asp Gly Ile Ala
210 215 220
Leu Leu Val Val Met Gly Val Val Leu Ile Leu Leu Val Ile Phe Gly
225 230 235 240
Tyr Ile Ser Ala Asp Arg Met Ala Lys Arg Gln Ser Glu Asp Ile Glu
245 250 255
Ala Ile Val Arg Lys Ile Asp Asp Ala Lys Asn Arg His Leu Gly Ser
260 265 270
Tyr Glu Pro Leu Lys Lys His Ser Glu Leu Glu Glu Ile Asn Asn Tyr
275 280 285
Ile Tyr Asp Leu Phe Glu Ser Asn Glu Gln Leu Ile Gln Ser Ile Glu
290 295 300
Gln Thr Glu Arg Arg Leu Arg Asp Ile Gln Leu Lys Glu Ile Glu Arg
305 310 315 320
Gln Phe Gln Pro His Phe Leu Phe Asn Thr Met Gln Thr Ile Gln Tyr
325 330 335
Leu Ile Pro Leu Ser Pro Lys Val Ala Gln Thr Val Ile Gln Gln Leu
340 345 350
Ser Gln Met Leu Arg Tyr Ser Leu Arg Thr Ala Ser His Thr Val Lys
355 360 365
Leu Ala Glu Glu Leu Ser Tyr Ile Glu Gln Tyr Val Ala Ile Gln Asn
370 375 380
Ile Arg Phe Asp Asp Met Ile Gln Leu Tyr Ile Asp Ala Thr Glu Gly
385 390 395 400
Val Gln His Gln Thr Ile Gly Lys Met Met Leu Gln Pro Leu Val Glu
405 410 415
Asn Ala Ile Lys His Gly Arg Asp Ser Glu Pro Leu Lys Ile Thr Ile
420 425 430
Arg Ile Arg Leu Thr Lys Arg Lys Leu His Ile Leu Val His Asp Asn
435 440 445
Gly Ile Gly Met Ser Pro Ser His Leu Glu His Val Arg Gln Ser Leu
450 455 460
His His Asp Val Phe Asp Thr Thr His Leu Gly Leu Asn His Leu His
465 470 475 480
Asn Arg Ala Met Ile Gln Tyr Gly Thr Tyr Ala Arg Leu His Ile Phe
485 490 495
Ser Arg Ser Gln Gln Gly Thr Leu Met Cys Tyr Gln Ile Pro Leu Val
500 505 510
<210>4
<211>346
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>4
Met Asn Asn Phe Arg Trp Phe Trp Phe Phe Ile Lys Ser Arg Ile Asn
1 5 10 15
Trp Ile Leu Trp Ile Leu Phe Leu Asn Ile Ile Leu Leu Gly Val Ala
20 25 30
Tyr Ile Asp Tyr Glu Ile Ser Val Glu Ser Val Phe Tyr Ile Val Ile
35 40 45
Leu Asn Val Gly Leu Ser Ile Leu Phe Leu Leu Phe Thr Phe Val Lys
50 55 60
Glu Val Arg Leu Ser Lys His Phe Tyr Glu Asp Lys Glu Ile Glu Glu
65 70 75 80
Ile Lys His Lys Asp Leu Ala Glu Thr Pro Phe Gln Gln Gln Val Ile
85 90 95
Asp Tyr Leu Tyr Arg His Ile Ala Ala Gln Lys Glu Lys Val Val Glu
100 105 110
Gln Gln Leu Gln Ile Lys Asn His Glu Gln Thr Ile Thr Glu Phe Val
115 120 125
His Asp Ile Lys Thr Pro Val Thr Ala Met Lys Leu Leu Ile Asp Gln
130 135 140
Glu Asn Asp Asp Gln Arg Lys Arg Ala Leu Leu Phe Glu Trp Ser Arg
145 150 155 160
Ile Asn Glu Met Leu Asp Lys Gln Leu Tyr Leu Thr Arg Leu Glu Thr
165 170 175
His His Arg Asp Met Tyr Phe Asp Tyr Ile Ser Leu Lys Arg Met Val
180 185 190
Ile Asp Glu Ile Gln Val Thr Arg His Ile Ser Gln Ala Lys Gly Ile
195 200 205
Gly Phe Glu Leu Asp Phe Lys Asp Glu Gln Lys Val Tyr Thr Asp Val
210 215 220
Lys Trp Cys Arg Met Met Ile Arg Gln Val Leu Ser Asn Ser Leu Lys
225 230 235 240
Tyr Ser Asp Asn Ser Thr Ile Asn Leu Ser Gly Tyr Asn Ile Glu Gly
245 250 255
His Val Val Leu Lys Ile Lys Asp Tyr Gly Arg Gly Ile Ser Lys Arg
260 265 270
Asp Leu Pro Arg Ile Phe Asp Arg Gly Phe Thr Ser Thr Thr Asp Arg
275 280 285
Asn Asp Thr Ala Ser Ser Gly Met Gly Leu Tyr Leu Val Gln Ser Val
290 295 300
Lys Glu Gln Leu Gly Ile Glu Val Lys Val Asp Ser Ile Val Gly Lys
305 310 315 320
Gly Thr Thr Phe Tyr Phe Ile Phe Pro Gln Gln Asn Glu Ile Ile Glu
325 330 335
Arg Met Ser Lys Val Thr Arg Leu Ser Phe
340 345
<210>5
<211>321
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>5
Met Trp Phe Asn Gly His Met Thr Leu Thr Leu Ala Ile Thr Thr Met
1 5 10 15
Ile Thr Ser Cys Leu Thr Leu Ser Ile Cys Ser Ile Phe Ile Asn Pro
20 25 30
Leu Ile Gln Lys Ile Lys Gln Phe Asn Ile Lys Thr Lys Gln Phe Ile
35 40 45
Asn His Glu Lys Phe Ile Asp Asp Glu Thr Phe Gln Ser Pro Arg Glu
50 55 60
Ile Lys Glu Leu Asn Asp Ser Phe Asn Lys Met Ala Tyr Glu Ile Asn
65 70 75 80
Asn Gln Met Asn Met Ile Lys Asn Glu Gln Gln Glu Lys Thr Glu Ile
85 90 95
Ile Gln Asn Leu Ala His Asp Leu Lys Thr Pro Leu Ala Gly Ile Arg
100 105 110
Ser Tyr Ser Glu Gly Leu Arg Asp Gly Val Ile Ser Asp Pro Gln Glu
115 120 125
Val His Glu Ala Tyr Glu Ile Leu Ile Lys Gln Ala Asn Arg Leu Ser
130 135 140
Ile Leu Phe Asp Asp Ile Thr His Val Ile Asn Leu Asn Thr Gly Arg
145 150 155 160
Ser Tyr Pro Leu Glu Leu Ile Gln Leu Asp Gln Leu Leu Val Asn Ile
165 170 175
Leu Gln Pro Tyr Glu Gln His Ile Lys Gln Glu Asn Arg Thr Leu Glu
180 185 190
Val Asn Phe Cys Thr Asp Ile Asp Ala Phe Tyr Gln Tyr Arg Pro Pro
195 200 205
Ile Glu Arg Ile Leu Thr Asn Leu Leu Asp Asn Ala Leu Lys Phe Ser
210 215 220
Asn Ser Gly Ser Arg Ile Asp Ile Ile Ile Ser Glu Cys Lys Glu Asn
225 230 235 240
Asp Val Ile Ser Ile Ser Ile Lys Asp Glu Gly Ile Gly Ile Val Pro
245 250 255
Glu Leu Gln Ser Arg Ile Phe Glu Arg Thr Phe Arg Val Glu Asp Ser
260 265 270
Arg Asn Thr Lys Thr Gly Gly Ser Gly Leu Gly Leu Tyr Ile Ala Asn
275 280 285
Glu Leu Ala Gln Gln Ile Asp Ala Ser Ile Thr Val Gln Ser Asp Leu
290 295 300
Asp Ile Gly Thr Thr Met Thr Leu Thr Leu Lys Lys Phe Gln Phe Lys
305 310 315 320
Lys
<210>6
<211>243
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>6
Met Glu Ser Arg Ser Trp Gly Ala Arg Ile Gly Glu Leu Ser Thr Leu
1 5 10 15
Ile Tyr Leu Ile Phe Pro Ile Leu Ala Ile Phe Val Asp Lys Arg Gly
20 25 30
Asn Leu Phe Thr Tyr Leu Ile Val Cys Thr Ile Phe Ile Ile Ser Tyr
35 40 45
Val Thr Met Ile Leu Phe Tyr Lys His Leu Ser Asp Ser Ile Leu Tyr
50 55 60
Ser Leu Leu Val Ile His Tyr Leu Gly Ile Phe Tyr Phe Val Tyr Ser
65 70 75 80
Val Asn Pro Met Asn Ser Leu Phe Phe Phe Tyr Ser Ala Phe Ala Leu
85 90 95
Pro Phe Ile Phe Asn Val Arg Val Val Ser Lys Glu Phe Ile Thr Phe
100 105 110
Leu Ile Ala Met Ile Ser Cys Leu Ile Leu Thr Tyr Ile Phe Asn Pro
115 120 125
Thr Phe Val Val Pro Leu Ser Ala Phe Tyr Leu Val Ile Leu Ile Val
130 135 140
Ala Val Gly Asn Phe Lys Asn Arg Asp Glu Arg Ile Met Lys Ala Lys
145 150 155 160
Leu Glu Glu Lys Asn Lys Tyr Ile Asn Val Leu Ile Ala Gln Gln Glu
165 170 175
Arg Asn Arg Ile Ser Gln Asp Leu His Asp Thr Leu Gly His Val Phe
180 185 190
Ala Ser Leu Thr Leu Lys Ser Glu Leu Ala Val Lys Leu Ile Asp Thr
195 200 205
Asn Pro Lys Glu Ala Lys Asn Glu Met Gln Ala Ile Asn Gln Leu Ser
210 215 220
Thr Glu Ala Leu Asn Lys Val Arg Leu Ile Ile Asp Asp Leu Lys Ile
225 230 235 240
Pro Ile Phe
<210>7
<211>456
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>7
Met Ile Lys Arg Gln Lys Leu Lys Tyr Lys Trp Met Leu Ile Thr Thr
1 5 10 15
Leu Ile Thr Phe Thr Thr Ile Leu Leu Phe Cys Leu Ile Ile Ile Phe
20 25 30
Phe Leu Lys Asp Thr Leu Arg Ser Ser Glu Ile Asp Glu Ala Glu Arg
35 40 45
Ser Ser Asn Asp Ile Ala Asn Leu Phe His Ser Lys Ser Leu Ser Asp
50 55 60
Ile Ser Ala Leu Asp Leu Asn Ala Ser Leu Glu Asn Phe Gln Glu Ile
65 70 75 80
Leu Ile Tyr Asp Asp Lys Gly Arg Lys Leu Ile Gln Thr Ser Asn Asp
85 90 95
Asn Thr Leu Ala Tyr Asp Asn Lys Ile Asp Phe Lys His Pro Glu Arg
100 105 110
Ile His Ile Gln Arg Ser His Gly Ile Asn Tyr Leu Val Ile Thr Glu
115 120 125
Pro Ile Arg Ser Lys Asp Phe Ser Gly Tyr Ser Val Leu Val His Ser
130 135 140
Leu Gln Asn Tyr Asp Asn Leu Val Lys Ser Leu Tyr Ile Val Ala Leu
145 150 155 160
Ala Phe Gly Leu Ile Ala Thr Ile Ile Thr Ala Gly Val Ser Tyr Ile
165 170 175
Phe Ser Ser Gln Ile Thr Lys Pro Ile Val Thr Met Ser Asn Lys Met
180 185 190
Asn Gln Ile Arg Arg Asp Gly Phe Gln Asn Lys Leu Glu Leu Thr Thr
195 200 205
Asn Tyr Glu Glu Thr Asp Asn Leu Ile Asp Thr Phe Asn Glu Met Met
210 215 220
Tyr Gln Ile Glu Glu Ser Phe Asn Gln Gln Arg Gln Phe Val Glu Asp
225 230 235 240
Ala Ser His Glu Leu Arg Thr Pro Leu Gln Ile Ile Gln Gly His Leu
245 250 255
Asn Leu Ile Gln Arg Trp Gly Lys Lys Asp Pro Ala Val Leu Glu Glu
260 265 270
Ser Leu Asn Ile Ser Ile Glu Glu Val Asn Arg Ile Thr Lys Leu Val
275 280 285
Glu Glu Leu Leu Leu Leu Thr Lys Asp Arg Val Asn His Asn Val Leu
290 295 300
Glu Cys Glu Asn Val Asp Val Asn Ser Glu Ile Gln Ser Arg Val Lys
305 310 315 320
Ser Leu Gln His Leu His Pro Asp Tyr Thr Phe Glu Thr His Leu Ala
325 330 335
Thr Lys Pro Ile Gln Leu Lys Ile Asn Arg His Gln Phe Glu Gln Leu
340 345 350
Leu Leu Ile Phe Ile Asp Asn Ala Met Lys Tyr Asp Thr Glu His Lys
355 360 365
His Ile Lys Ile Val Thr Gln Leu Lys Asn Lys Met Ile Met Ile Asp
370 375 380
Ile Thr Asp His Gly Met Gly Ile Pro Lys Ala Asp Leu Glu Phe Ile
385 390 395 400
Phe Asp Arg Phe Tyr Arg Val Asp Lys Ser Arg Ala Arg Ser Gln Gly
405 410 415
Gly Asn Gly Leu Gly Leu Ser Ile Ala Glu Lys Ile Val Gln Leu Asn
420 425 430
Gly Gly Met Ile Gln Val Glu Ser Glu Leu Gln Lys Tyr Thr Thr Phe
435 440 445
Lys Ile Ser Phe Pro Val Leu Asn
450 455
<210>8
<211>589
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>8
Met Met Asn Arg Leu Asn Ser Val Val Ile Lys Leu Trp Leu Thr Ile
1 5 10 15
Ile Leu Ile Val Ala Thr Val Leu Ile Leu Leu Ser Val Ser Leu Ile
20 25 30
Thr Phe Ile Gln Tyr Tyr Phe Thr Gln Glu Thr Glu Asn Ala Ile Lys
35 40 45
Glu Asp Ala Lys Arg Ile Ser Ser Leu Val Glu Lys Ser His Asn Lys
50 55 60
Thr Leu Ala Ile Gln Asn Ser Gln Thr Leu Ile Asp Gly Asp Gly Pro
65 70 75 80
Gly Gly Leu Ile Ile Met Lys Asp Met Ser Glu Lys Pro Asp Ser Ser
85 90 95
Tyr Lys Asp Thr Lys Lys Gln Met Phe Asn Glu Ile Lys Lys Ser Thr
100 105 110
Lys Phe Lys Lys Val Phe Lys Glu Gly Glu Tyr Glu Thr Gln Asn Ile
115 120 125
Thr Ile Lys Asn Lys Gly Asn Ser Gln Ser Tyr Leu Leu Leu Gly Tyr
130 135 140
Pro Met Lys Ala Gln Lys Gly Ala Gln Ser His Tyr Ser Gly Val Phe
145 150 155 160
Ile Tyr Lys Asp Leu Lys Ser Ile Glu Asp Thr Asn Asn Ala Ile Thr
165 170 175
Ile Ile Ile Leu Ile Thr Ala Ile Ile Phe Thr Ile Ala Ser Thr Ile
180 185 190
Phe Ala Phe Phe Leu Ser Asn Arg Ile Thr Lys Pro Leu Arg Gln Leu
195 200 205
Lys Thr Gln Ala Gln Lys Val Ser Glu Gly Asp Tyr Ser Gln Ile Ser
210 215 220
Thr Val Ala Thr Lys Asp Glu Ile Gly Asp Leu Ser Arg Ala Phe Asn
225 230 235 240
Asn Met Asn Val Glu Ile Gln Glu His Ile Lys Ala Ile Ser Ser Ser
245 250 255
Lys Asn Ile Arg Asp Thr Leu Leu Asn Ser Met Val Glu Gly Val Leu
260 265 270
Gly Ile Asn Asn Gln Arg Glu Ile Ile Leu Ser Asn Lys Met Ala Asp
275 280 285
Asp Ile Met Arg His Ile Asp Asp Phe Ser Lys Glu Ser Ile Glu Gln
290 295 300
Gln Ile Glu Ala Thr Phe Glu Ser Gln Gln Asn Glu Tyr Leu Glu Leu
305 310 315 320
Glu Ile Asn Thr Arg Tyr Tyr Val Phe Ile Ser Ser Tyr Ile Asp Arg
325 330 335
Ile Gln Thr Asn Gly Arg Ser Gly Ile Val Met Val Ile Arg Asp Met
340 345 350
Thr Asn Glu His Asn Leu Asp Gln Met Lys Lys Asp Phe Ile Ala Asn
355 360 365
Val Ser His Glu Leu Arg Thr Pro Ile Ser Leu Leu Gln Gly Tyr Thr
370 375 380
Glu Ser Ile Val Asp Gly Ile Val Thr Glu Pro Asp Glu Ile Arg Asp
385 390 395 400
Ser Leu Ala Ile Val Leu Asp Glu Ser Lys Arg Leu Asn Arg Leu Val
405 410 415
Asn Glu Leu Leu Asn Val Ala Arg Met Asp Ala Glu Gly Leu Ser Val
420 425 430
Glu Lys Glu Leu Gln Pro Ile Gln His Leu Leu Asp Lys Met Glu Ser
435 440 445
Lys Tyr Arg Met Gln Ser Glu Glu Leu Gly Leu Thr Met Thr Phe Asp
450 455 460
Ser Asn Asn Asp Glu Gln Leu Trp Asn Tyr Asp Met Asp Arg Met Asp
465 470 475 480
Gln Val Leu Thr Asn Leu Ile Asp Asn Ala Thr Arg Tyr Thr Gln Ala
485 490 495
Gly Asp Ser Ile Lys Ile Ser Ile Asp Glu Asp Ser Asp Phe Asn Ile
500 505 510
Leu Thr Ile Thr Asp Thr Gly Thr Gly Ile Ala Pro Glu His Leu Lys
515 520 525
Gln Val Phe Asp Arg Phe Tyr Lys Val Asp Ala Ala Arg Lys Arg Gly
530 535 540
Lys Gln Gly Thr Gly Leu Gly Leu Phe Ile Cys Lys Met Ile Ile Glu
545 550 555 560
Glu His Gly Gly Arg Ile Asp Val Glu Ser Glu Leu Gly Lys Gly Thr
565 570 575
Ser Phe Ile Ile Arg Leu Pro Lys Ser Lys Gln Ile Ser
580 585
<210>9
<211>565
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>9
Met Leu Lys Phe His Leu Arg Leu Leu Leu Leu Ile Ser Thr Ile Thr
1 5 10 15
Ile Ile Ser Phe Ile Gly Leu Gly Ala Ile Ile His Asn Thr Ile Tyr
20 25 30
Gln Thr Leu Thr Ser Asn Gln Ile Lys Ser Leu Asp Ser Glu Ala Arg
35 40 45
Asn Tyr Val Asn Leu Phe Asn Asn Asn Lys Glu Lys Glu Ile Thr Asn
50 55 60
Ile Ala His Asn Glu Lys Asn Ile Ile Leu Ile Lys Glu Lys Asp Lys
65 70 75 80
Asp Lys Ile Ile Tyr Ser Ser Gly Asn Ile Lys Asp Ile Asp His Arg
85 90 95
Ile Asp Asn Glu Ala Asn Pro Ser Lys Leu Ile Asn Lys Asn Thr Lys
100 105 110
Leu Gly Met Arg Tyr Thr Tyr Lys Asn Thr Ile Asp Asp Lys Thr Ile
115 120 125
Tyr Ile Ser Gly Ile Asn Asn Glu Ile Ile Asp Leu Gln Lys Asp Leu
130 135 140
Trp Lys Tyr Leu Ser Ile Val Gly Val Ile Val Leu Phe Thr Val Tyr
145 150 155 160
Leu Ala Ser Arg Ser Ile Asn Arg Thr Tyr Ile Arg Pro Ile Asn Glu
165 170 175
Val Thr Tyr Ala Thr Ser Leu Leu Ala Asp Gly Tyr Tyr His Val Arg
180 185 190
Val Pro Glu Ser Asn Val Lys Glu Thr Arg Ala Leu Phe Val Thr Thr
195 200 205
Asn Asp Leu Ala Arg Arg Leu Gln Lys Leu Asn Asn Ser Gln Lys Ile
210 215 220
Gln Ser Asn Arg Leu Lys Thr Thr Leu Glu Asn Ile Pro Ser Ser Val
225 230 235 240
Leu Met Ile Asp Lys His Gly Glu Ile Val Val Ala Asn His Ala Tyr
245 250 255
Tyr Gln Val Phe Asn Pro Asp Gln Met Val Glu Asn Lys Ser Tyr Ile
260 265 270
Gly Phe Ile Asp Asp Ser Ile Glu Lys Leu Ile Ile Glu Ser Phe Arg
275 280 285
Thr Glu Lys Val Ile Tyr Glu Gln Leu Glu Val Ala Ile Asn Asn Val
290 295 300
His Thr Lys Tyr Phe Asp Val Ser Cys Ile Pro Ile Leu Thr Lys Ser
305 310 315 320
Lys Lys Asn Leu Gln Gly Met Val Val Val Leu His Asp Ile Thr Asn
325 330 335
Leu Gln Lys Leu Glu Asn Leu Arg Arg Glu Phe Val Ala Asn Val Ser
340 345 350
His Glu Leu Lys Thr Pro Ile Thr Ser Ile Lys Gly Phe Ala Glu Thr
355 360 365
Leu Ile Glu Gly Ala Lys Asn Asp Glu Gln Ser Leu Asp Met Phe Leu
370 375 380
Asn Ile Ile Leu Lys Glu Ser Asn Arg Ile Glu Ser Leu Val Thr Asp
385 390 395 400
Leu Leu Asp Leu Ser His Ile Glu Gln Gln Lys Glu Leu Glu Ile Asn
405 410 415
Tyr Met Asn Leu Ser Glu Leu Ala Ile Asn Ile Ile Asp Asn Leu Gln
420 425 430
Thr Gln Ala Tyr Asn Lys Arg Ile Lys Ile Gln Ser Glu Ile Glu Lys
435 440 445
Asp Val Ile Ile Glu Ala His Glu Asn Lys Ile Ala Gln Val Ile Thr
450 455 460
Asn Leu Leu Ser Asn Ala Ile Asn Tyr Ser Ser Glu Asp Asn Lys Val
465 470 475 480
Ile Val Arg Val Tyr Arg Asn Asp Asn Lys Val Tyr Leu Glu Ile Gln
485 490 495
Asp Tyr Gly Ile Gly Ile Ser Glu Thr Asp Gln Lys Arg Ile Phe Glu
500 505 510
Arg Phe Tyr Arg Val Asp Lys Ala Arg Ser Arg Asp Ser Gly Gly Thr
515 520 525
Gly Leu Gly Leu Ser Ile Thr Lys His Ile Val Glu Ala His Asn Gly
530 535 540
Arg Ile Asp Val Lys Ser Ala Pro Gly Lys Gly Ser Ile Phe Lys Val
545 550 555 560
Leu Phe Asn Asp Asn
565
<210>10
<211>377
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>10
Met Glu Lys Pro Thr Arg Leu Ala Leu Leu Lys Glu Ile Ala Glu Phe
1 5 10 15
Leu Asn Glu Glu Thr Glu Ile Tyr Ser Met Met Gln Gly Ala Leu Arg
20 25 30
Leu Leu Ile Asn Gly Ser Asp Phe Ser Thr Gly Trp Ile Phe Tyr Ile
35 40 45
Asp Glu Glu Gly His His Asp Leu Val Ser Ser Val Asp Leu Pro Glu
50 55 60
Ala Leu Ser Lys Asn His Cys Tyr Tyr Leu Thr Asn Gly Ser Cys Trp
65 70 75 80
Cys Val Gln Ala Tyr Gln Asn His Lys Leu Thr Lys Ala Ser Asn Ile
85 90 95
Ile Asn Cys Ser Arg Ile Asn Leu Ala Asn His Ala Tyr Asp Lys Glu
100 105 110
Thr Asn His Ile Thr His His Ala Thr Val Pro Leu Gln Ser Gly Ser
115 120 125
Glu Arg Phe Gly Leu Leu Asn Val Ala Ser Pro Asn Lys Glu His Tyr
130 135 140
Ser Glu Glu Asp Leu Glu Leu Leu Glu Ser Val Ala Phe Gln Ile Gly
145 150 155 160
Ser Ala Ile Lys Arg Ile Gln Leu Thr Asn Lys Glu Lys Glu Asn Ala
165 170 175
Lys Ile Asn Glu Arg Asn Arg Leu Ala Arg Asp Leu His Asp Ser Val
180 185 190
Asn Gln Met Leu Phe Ser Leu Lys Leu Thr Ala His Ala Ala Lys Gly
195 200 205
Ile Thr Thr Asp Glu Gln Ala Gln Arg Ala Phe Lys Thr Ile Glu Glu
210 215 220
Thr Ser Gln Asn Ala Val Asn Glu Met Arg Ala Leu Ile Trp Gln Leu
225 230 235 240
Lys Pro Val Gly Leu Glu Gln Gly Leu Ile His Ala Leu His Tyr Tyr
245 250 255
Ser Lys Met Leu Gly Leu Asp Leu Lys Val Thr Ile Asn Gly Leu Ile
260 265 270
Asp Leu Ser Asn Asp Ile Glu Glu Asn Val Tyr Arg Ile Ile Gln Glu
275 280 285
Ala Met Asn Asn Val Ile Lys His Ala Lys Thr Asn Lys Val Tyr Leu
290 295 300
Asp Leu Ser Gln Asn Val Asp Tyr Leu Glu Val Asp Ile Arg Asp Asn
305 310 315 320
Gly Gln Gly Phe Asp Thr Gln Ser Ile Asn Thr Phe Leu Phe Asn Gly
325 330 335
Leu Lys Asn Ile Lys Gln Arg Thr Gln Tyr Leu Asn Gly Lys Val Thr
340 345 350
Ile Glu Ser Lys Ile Asn Gln Gly Thr Arg Ile Tyr Ile Lys Ile Pro
355 360 365
Leu Lys Leu Lys Glu His Pro Tyr Ala
370 375
<210>11
<211>348
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>11
Met Asn His Tyr Ile Arg Ala Ile Gly Ser Met Leu IIe Leu Val Tyr
1 5 10 15
Ser Met Leu Ile Ala Phe Leu Phe Ile Asp Lys Val Phe Val Asn Ile
20 25 30
Ile Phe Phe Gln Gly Met Phe Tyr Thr Gln Ile Phe Gly Ile Pro Val
35 40 45
Phe Leu Phe Leu Asn Leu Leu Ile Val Leu Leu Cys Ile Ile Val Gly
50 55 60
Ser Val Leu Ala Tyr Lys Ile Asn Gln Gln Asn Asp Trp Ile Ile Ser
65 70 75 80
Gln Ile Glu Arg Ser Ile Glu Gly Gln Thr Val Gly Ile Asn Asp Gln
85 90 95
Asn Ile Glu Leu Tyr Thr Glu Thr Ile Asp Ile Tyr His Thr Leu Val
100 105 110
Pro Leu Asn Gln Glu Leu His Arg Leu Arg Met Lys Thr Gln Asn Leu
115 120 125
Thr Asn Glu Asn Tyr Asn Ile Asn Asp Val Lys Val Lys Lys Ile Ile
130 135 140
Glu Asp Glu Arg Gln Arg Leu Ala Arg Glu Leu His Asp Ser Val Ser
145 150 155 160
Gln Gln Leu Phe Ala Ala Ser Met Met Leu Ser Ala Ile Lys Glu Ser
165 170 175
Lys Leu Glu Pro Pro Leu Asn Gln Gln Ile Pro Ile Leu Glu Lys Met
180 185 190
Val Gln Asp Ser Gln Leu Glu Met Arg Ala Leu Leu Leu His Leu Arg
195 200 205
Pro Ile Gly Leu Lys Asp Lys Ser Leu Gly Glu Gly Ile Lys Asp Leu
210 215 220
Val Ile Asp Leu Gln Lys Lys Val Pro Met Lys Val Val His Glu Ile
225 230 235 240
Gln Asp Phe Glu Val Pro Lys Gly Ile Glu Asp His Leu Phe Arg Ile
245 250 255
Thr Gln Glu Ala Ile Ser Asn Thr Leu Arg His Ser Asn Gly Thr Lys
260 265 270
Val Thr Val Glu Leu Phe Asn Gln Glu Asp Tyr Leu Leu Leu Arg Ile
275 280 285
Gln Asp Asn Gly Lys Gly Phe Asn Val Asp Glu Lys Phe Glu Gln Ser
290 295 300
Tyr Gly Leu Lys Asn Met Arg Glu Arg Ala Leu Glu Ile Gly Ala Thr
305 310 315 320
Phe His Ile Val Ser Leu Pro Asp Ser Gly Thr Arg Ile Glu Val Lys
325 330 335
Ala Pro Leu Asn Lys Glu Glu Asn Ser Ser Gly Asp
340 345
<210>12
<211>429
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>12
Met Asp Asp Ile Asn Leu Phe Pro Phe Ala Gly Leu Gln Ile Phe Leu
1 5 10 15
Met Ile Trp Val Thr Lys Val Ile Ile Asn Met Lys Phe Asn Phe Arg
20 25 30
Asp Tyr Ile Ile Val Phe Thr Ile Val Ile Pro Ser Ala Ile Met Tyr
35 40 45
Tyr Phe Trp Gln Ser Lys Ala Leu Ile Val Leu Val Ile Ile Ile Thr
50 55 60
Ile Phe Phe Tyr Thr Lys Ile Lys Leu Tyr Ser Ile Leu Val Val Leu
65 70 75 80
Phe Thr Thr Met Ile Leu Tyr Ile Thr Asn Phe Ile Thr Val Tyr Ile
85 90 95
His Leu Thr Ile Lys Asp Tyr Ile Pro Phe Lys Phe Ala Leu Gln Leu
100 105 110
Ile His Phe Thr Ser Phe Val Ile Ile Thr Leu Ile Ile Ala Tyr Leu
115 120 125
Thr Gln Leu Leu Phe Asn Lys Leu Lys Val Ser Tyr Leu Ser Leu Asn
130 135 140
Lys Arg Tyr Leu Leu Ile Ile Thr Ile Val Leu Phe Ile Ser Phe Ile
145 150 155 160
Leu Leu Tyr Met Val Ser Gln Thr Asp Met Arg Gly Asn Asp Thr Leu
165 170 175
Lys Leu Tyr Ala Ile Leu Leu Leu Gly Ile Met Val Phe Leu Ser Val
180 185 190
Val Ile Leu Val Met Ser Asn Phe Thr Leu Arg Glu Met Arg Tyr Lys
195 200 205
Arg Asn Val Lys Glu Ile Glu Ala Tyr Tyr Glu Tyr Thr Leu Arg Ile
210 215 220
Glu Ser Ile Asn Asn Glu Met Arg Lys Phe Arg His Asp Tyr Val Asn
225 230 235 240
Ile Leu Thr Thr Leu Ser Asp Tyr Ile Arg Glu Asp Asp Met Pro Gly
245 250 255
Leu Arg Lys Tyr Phe Asn Glu Asn Ile Val Pro Met Lys Asp Lys Leu
260 265 270
Lys Thr Arg Ser Ile Lys Met Asn Gly Ile Glu Lys Leu Lys Val Arg
275 280 285
Glu Ile Lys Gly Leu Ile Thr Thr Lys Ile Ile Gln Ala Gln Glu Lys
290 295 300
Arg Ile Pro Ile Ser Ile Glu Val Pro Asp Glu Ile Asp Arg Ile Asp
305 310 315 320
Met Asn Thr Val Glu Leu Ser Arg Ile Ile Gly Ile Ile Val Asp Asn
325 330 335
Ala Ile Glu Ala Ser Glu Asn Leu Glu Glu Pro Leu Ile Asn Ile Ala
340 345 350
Phe Ile Asp Asn Glu Glu Ser Val Thr Phe Ile Val Met Asn Lys Cys
355 360 365
Ser Asn Asp IlePro Lys Ile His Glu Leu Phe Glu Gln Gly Phe Ser
370 375 380
Thr Lys Gly Asp Asn Arg Gly Leu Gly Leu Ser Thr Leu Lys Glu Leu
385 390 395 400
Thr Asp Ser Asn Glu Asn Val Leu Leu Asp Thr Val Ile Glu Asn Gly
405 410 415
Tyr Phe Val Gln Lys Val Glu Ile Asn Asn Lys Glu Ser
420 425
<210>13
<211>451
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>13
Met Phe Lys Thr Leu Tyr Ser Arg Ile Ala Ile Tyr Ala Ile Thr Val
1 5 10 15
Ile Leu Phe Ser Ala Leu Met Ser Phe Leu Phe Thr Asn Ile Tyr Tyr
20 25 30
His Phe His Leu Lys Ala Ser Asn Asp Ala Lys Ile Met Arg Thr Leu
35 40 45
Lys Glu Ala Arg Glu Tyr Glu Arg Thr Gln Lys Pro Lys Pro Leu Asp
50 55 60
Thr Tyr Leu Lys His Leu Gly Gln Met Asn Tyr Gln Ile Met Thr Val
65 70 75 80
Asn Glu His Gly Thr Lys His Phe Tyr Gly Glu Thr Phe Arg Lys Asn
85 90 95
Thr Ile Ser Gln Ser Ala Ile Lys Lys Val Leu Asn Gly Glu Asp Tyr
100 105 110
His Gly Ile Lys Asn Lys Pro Tyr Ala Phe Phe Val Thr Gly Phe Phe
115 120 125
Asp Asn Glu Thr Asp Asn Thr Val Gly Ile Gln Phe Lys Thr Asp Asp
130 135 140
Gly Ala Leu Ala Val Phe Met Arg Pro Asp Ile Gly Lys Thr Phe Ser
145 150 155 160
Glu Phe Arg Ile Phe Leu Ala Val Leu Ile Thr Leu Leu Leu Ile Ile
165 170 175
Ser Ile Ser Leu Val Ile Ala Ser Thr Tyr Ser Ile Ile Lys Pro Val
180 185 190
Thr Ala Leu Lys Asn Ala Thr Thr Arg Ile Met Lys Gly Asp Phe Ser
195 200 205
Thr Pro Ile Lys Gln Thr Arg His Asp Glu Ile Gly Thr Leu Gln Ser
210 215 220
Arg Phe Asn Thr Met Arg Gln Asn Leu Gly Gln Val Asp Gln Met Arg
225 230 235 240
Gln His Phe Val Gln Asn Val Ser His Glu Ile Lys Thr Pro Leu Thr
245 250 255
His Leu Gln Arg Leu Leu Thr Gln Leu Glu Leu Thr Gln Asn Glu Glu
260 265 270
Glu Lys Gln Leu Cys Ile Asn Glu Met Phe Glu Ile Thr Asn Gln Val
275 280 285
Ser Glu Leu Thr Lys Glu Leu Leu Leu Leu Ser Glu Leu Asp Asn Ala
290 295 300
Ser His Leu Thr Phe Asn Asp Asn Val His Leu Asn Thr Leu Ile Lys
305 310 315 320
Asp Ile Ile Arg His Glu Gln Phe Arg Thr Asp Glu Lys Asp Leu Val
325 330 335
Leu Phe Thr Glu Leu Glu Asp Leu Tyr Phe Arg Gly Asn Glu Arg Leu
340 345 350
Leu His Gln Ala Phe Asn Asn Leu Ile Ile Asn Ala Met Lys Tyr Ala
355 360 365
Pro Gln Asn Ser Met Ile Asn Ile Thr Leu Thr Ser Thr Asn His Leu
370 375 380
Ile Ile Phe Asn Ile Glu Asn Asp Gly Ser Ile Ala Glu Glu Asp Ala
385 390 395 400
Lys His Ile Phe Asp Arg Phe Tyr Lys Leu Ser Asp Glu Ser Ser Ser
405 410 415
Asn Gly Leu Gly Leu Ala Ile Thr Gln Ser Ile Ile His Leu His His
420 425 430
Gly Ser Ile Thr Leu Thr Ser Asp Asp Lys Thr Gln Phe Ile Val Lys
435 440 445
Leu Phe Ile
450
<210>14
<21l>344
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>14
Met Leu Glu Gln Thr Asp Leu Ser Leu Glu Gln Leu Leu Lys Asn Tyr
1 5 10 15
Tyr Glu Thr Thr Asn Glu Lys Ile Val Phe Val Asn Arg Gln Gly Lys
20 25 30
Ile Ile Ala Met Asn Asp Ala Ala Lys Asp Ile Leu Thr Glu Glu Asp
35 40 45
Asn Tyr Asn Ala Met Thr Asn Ala Ile Cys His Arg Cys Glu Gly Tyr
50 55 60
Ser Asn Glu Tyr Asp Val Gln Ser Cys Lys Asp Cys Phe Leu Glu Thr
65 70 75 80
Thr Gln Leu Gln His Ser Asn Phe Gln Val Phe Met Lys Thr Lys Asp
85 90 95
Asn Glu Ile Lys Pro Phe Thr Ala Met Tyr Gln Asn Ile Asp Glu Gln
100 105 110
Arg Gly Ile Ser Ala Phe Thr Leu Gln Asn Val Ala Pro Gln Ile Glu
115 120 125
Arg Gln Glu Lys Met Tyr Gln Gln Lys Met Leu His Arg Ser Ile Gln
130 135 140
Ala Gln Glu Asn Glu Arg Lys Arg Ile Ser Arg Glu Leu His Asp Ser
145 150 155 160
Val Ile Gln Asp Met Leu Asn Ile Asp Val Glu Leu Arg Leu Leu Lys
165 170 175
Tyr Lys His Arg Asp Lys Val Leu Ala Glu Thr Ser Gln Arg Ile Glu
180 185 190
Gly Leu Leu Ser Gln Leu Ile Asp Asp Ile Arg Asn Met Ser Val Glu
195 200 205
Leu Arg Pro Ser Ser Leu Asp Asp Leu Gly Ile Glu Ala Ala Phe Lys
210 215 220
Ser Tyr Phe Lys Gln Phe Glu Glu Asn Tyr Gly Met His Ile Lys Tyr
225 230 235 240
Asp Ser Asn Ile Lys Gly Met Arg Phe Asp Asn Glu Ile Glu Thr Val
245 250 255
Val Tyr Arg Val Val Gln Glu Gly Val Phe Asn Ala Leu Lys Tyr Ala
260 265 270
Glu Val Asn Glu Ile Glu Val Ser Thr His Ser Asp Gly Lys Gln Leu
275 280 285
Val Ala Glu Val Val Asp Arg Gly Lys Gly Phe Ser Leu Asp His His
290 295 300
Pro Lys Gly Ser Gly Leu Gly Leu Tyr Gly Met Arg Glu Arg Ala Glu
305 310 315 320
Leu Val Asn Gly His Val Asn Ile Glu Thr His Ile Asn Arg Gly Thr
325 330 335
Ile Ile Thr Leu Asp Ile Pro Ile
340
<210>15
<211>591
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>15
Met Leu Asn Leu Phe Ile Leu Leu Leu Glu Arg Val Gly Leu Ile Ile
1 5 10 15
Leu Leu Ala Tyr Ile Leu Met Asn Ile Asn His Phe Lys Thr Met Met
20 25 30
Ser Glu Arg Asp Lys Trp Arg Ser Lys Phe Gln Leu Ile Ile Ile Phe
35 40 45
Gly Ile Phe Ser Met Ile Ser Asn Phe Thr Gly Ile Glu Ile Glu Asn
50 55 60
Gly His Ile Val Ser Ser Asp Ile Tyr Tyr His Leu Ser Lys Asp Ala
65 70 75 80
Ser Met Ala Asn Thr Arg Val Leu Thr Ile Gly Val Ser Gly Leu Ile
85 90 95
Gly Gly Pro Trp Val Ala Ile Ile Val Gly Ile Ile Ser Gly Leu Cys
100 105 110
Arg Leu Tyr Ile Gly Gly Ala Asp Ala Tyr Thr Tyr Leu Ile Ser Ser
115 120 125
Ile Val Ile Ala Ile Ile Ser Gly Tyr Phe Gly His Gln Thr Ile Lys
130 135 140
Gln Asn Thr Tyr Pro Ser Ile Lys Lys Gly Ala Ile Ile Gly Ala Ile
145 150 155 160
Thr Glu Ile Ile Gln Met Gly Cys Ile Leu Leu Phe Thr Asn Asn Leu
165 170 175
His His Ala Ile Thr Leu Val Ser Phe Ile Ala Leu Pro Met Ile Ile
180 185 190
Ile Asn Ser Leu Gly Thr Ala Ile Phe Leu Thr Ile Ile Leu Ser Thr
195 200 205
Ile Lys Gln Glu Glu Gln Met Arg Ala Val Gln Thr His Asp Val Leu
210 215 220
Gln Leu Ala Asn Glu Thr Leu Pro Tyr Phe Arg Ser Gly Leu Asn Glu
225 230 235 240
Lys Ser Ala Gln Gln Ala Ala Glu Ile Ile Leu Lys Leu Met Gln Val
245 250 255
Ser Ala Val Ala Ile Thr Asn Lys Lys Asp Ile Leu Thr His Ile Gly
260 265 270
Ala Gly Ser Asp His His Val Ala Arg Lys Glu Ile Ile Thr Asp Leu
275 280 285
Ser Lys Glu Val Ile Gln Ser Gly Lys Leu Lys Val Ala His Thr Arg
290 295 300
Glu Gly Ile Gly Cys His His Pro Asn Cys Pro Leu Glu Gly Ala Ile
305 310 315 320
Val Val Pro Leu Tyr Ile His Asn Glu Val Ala Gly Thr Leu Lys Phe
325 330 335
Tyr Phe Thr Asp Asn Asn Ile Ile Ser Thr Ser Asp Gln Gln Leu Ala
340 345 350
Lys Gly Leu Ala Asn Ile Phe Ser Ser Gln Leu Glu Leu Gly Gln Ala
355 360 365
Glu Met Gln Gly Gln Leu Leu Lys Asp Ala Glu Ile Lys Ser Leu Gln
370 375 380
Ala Gln Val Asn Pro His Phe Phe Phe Asn Ala Ile Asn Thr Ile Ser
385 390 395 400
Ala Leu Val Arg Ile Asp Ser Glu Lys Ala Arg Arg Leu Leu Ile Gln
405 410 415
Leu Ser Gln Phe Phe Arg Ser Asn Leu Asn Gly Ala Arg Asn Asn Thr
420 425 430
Ile Thr Leu Gln Lys Glu Leu Gln Gln Val Ala Ala Tyr Leu Ser Leu
435 440 445
Glu Gln Ala Arg Tyr Pro Asn Arg Phe Asn Ile His Tyr Arg Ile Asp
450 455 460
Asp Gln Cys Gln Asp Ala Leu Ile Pro Pro Phe Ile Ile Gln Ile Leu
465 470 475 480
Val Glu Asn Ser Ile Lys His Ala Phe Lys Asn Arg Lys Lys Asn Asn
485 490 495
His Ile Asp Val Asp Val Ser Met Lys Gln Asp Cys Leu Ser Ile Ser
500 505 510
Val Gln Asp Asn Gly Gln Gly Ile Pro Ala Asp Gln Leu Asp Thr Ile
515 520 525
Gly Tyr Thr Thr Val Thr Ser Thr Thr Gly Thr Gly Asn Ala Leu Val
530 535 540
Asn Leu Asn Lys Arg Leu Thr Gly Leu Phe Gly Thr Thr Ser Ala Leu
545 550 555 560
Asn Ile Gln Ser Ser Gln Ser Gly Thr Thr Val Ser Cys Leu Ile Pro
565 570 575
Tyr Lys Ser Ser Lys Glu Glu His Phe Asn Glu Ser Val Asn Arg
580 585 590
<210>16
<211>298
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>16
Met Lys Phe Ala Tyr Ile Gln Ser Ile Arg Asn Glu Ile Ser Ile Ile
1 5 10 15
Leu Ile Ile Leu Leu Phe Phe Ala Leu Ile Phe Tyr Val Phe Ser Leu
20 25 30
Pro Phe Asp Ala Tyr Val Leu Ala Ile Ser Ile Ile Leu Leu Leu Met
35 40 45
Cys Val Arg Trp Trp Ile Lys Tyr Leu Ser Phe Lys Lys Asn Glu His
50 55 60
Leu Lys Asp Lys Val Ala Tyr Leu Glu His Glu Leu Ala His Val Lys
65 70 75 80
Asn Gln Gln Ile Glu Tyr Arg Asn Asp Val Glu Ser Tyr Phe Leu Thr
85 90 95
Trp Val His Gln Ile Lys Thr Pro Ile Thr Ala Ser Gln Leu Leu Leu
100 105 110
Glu Arg Ash Glu Glu Asn Val Val Asn Arg Val Arg Gln Glu Ile Val
115 120 125
His Ile Asp Asn Tyr Thr Ser Leu Ala Leu Ser Tyr Leu Lys Leu Leu
130 135 140
Asn Glu Glu Ser Asp Met Thr Ile Thr Lys Val Thr Val Asp Asp Leu
145 150 155 160
Ile Arg Pro Leu Ile Leu Lys Tyr Arg Ile Gln Phe Ile Glu Gln Lys
165 170 175
Thr Gln Ile His Tyr Glu Lys Ser Glu Asp Ile Ile Leu Thr Asp Ala
180 185 190
Gln Trp Ala Ser Ile Met Ile Glu Gln Leu Leu Asn Asn Ala Leu Lys
195 200 205
Tyr Ala Lys Gly Lys Asp Ile Trp Ile Asp Phe Asp Val Ala Asn Gln
210 215 220
Thr Leu Gln Ile Lys Asp Asn Gly Ile Gly Ile Ser Lys Ala Asp Ile
225 230 235 240
Pro Lys Ile Phe Asp Lys Gly Tyr Ser Gly Phe Asn Gly Arg Leu Asn
245 250 255
Glu Gln Ser Thr Gly Ile Gly Leu Phe Ile Val Gln His Ile Ala Asn
260 265 270
His Leu Asn Ile Gln Val Thr Val Gln Ser Glu Leu Asn His Gly Thr
275 280 285
Val Phe Phe Ile His Phe Thr Lys Glu Lys
290 295
<210>17
<211>233
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>17
Met Ala Arg Lys Val Val Val Val Asp Asp Glu Lys Pro Ile Ala Asp
1 5 10 15
Ile Leu Glu Phe Asn Leu Lys Lys Glu Gly Tyr Asp Val Tyr Cys Ala
20 25 30
Tyr Asp Gly Asn Asp Ala Val Asp Leu Ile Tyr Glu Glu Glu Pro Asp
35 40 45
Ile Val Leu Leu Asp Ile Met Leu Pro Gly Arg Asp Gly Met Glu Val
50 55 60
Cys Arg Glu Val Arg Lys Lys Tyr Glu Met Pro Ile Ile Met Leu Thr
65 70 75 80
Ala Lys Asp Ser Glu Ile Asp Lys Val Leu Gly Leu Glu Leu Gly Ala
85 90 95
Asp Asp Tyr Val Thr Lys Pro Phe Ser Thr Arg Glu Leu Ile Ala Arg
100 105 110
Val Lys Ala Asn Leu Arg Arg His Tyr Ser Gln Pro Ala Gln Glu Val
115 120 125
Ser Gly Ala Thr Asn Glu Ile Thr Ile Lys Asp Ile Val Ile Tyr Pro
130 135 140
Asp Ala Tyr Ser Ile Lys Lys Arg Gly Glu Asp Ile Glu Leu Thr His
145 150 155 160
Arg Glu Phe Glu Leu Phe His Tyr Leu Ser Lys His Met Gly Gln Val
165 170 175
Met Thr Arg Glu His Leu Leu Gln Thr Val Trp Gly Tyr Asp Tyr Phe
180 185 190
Gly Asp Val Arg Thr Val Asp Val Thr Ile Arg Arg Leu Arg Glu Lys
195 200 205
Ile Glu Asp Asp Pro Ser His Pro Glu Tyr Ile Val Thr Arg Arg Gly
210 215 220
Val Gly Tyr Phe Leu Gln Gln His Asp
225 230
<210>18
<211>222
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>18
Met Met Tyr Asn Ile Met Ile Val Asp Asp Glu Glu Lys Ile Val Ser
1 5 10 15
Phe Ile Glu Glu Ser Leu Tyr Leu Glu Gly Phe Asn Thr Ile Val Ala
20 25 30
Tyr Asn Gly Arg Asp Val Leu Lys Gln Ile Asp Asp Lys Ile Asp Leu
35 40 45
Ile Ile Leu Asp Ile Lys Met Pro Tyr Ile Asp Gly Phe Glu Val Ala
50 55 60
Gln Ser Leu Lys Glu Ser Asn Ile Pro Ile Ile Phe Leu Thr Ala Lys
65 70 75 80
Asp Asn Leu Asp Thr Arg Leu Lys Cys Phe Ser Leu Gly Ala Lys Asp
85 90 95
Tyr Leu Pro Lys Pro Phe Tyr Met Glu Glu Leu Ile Ile Arg Ile Lys
100 105 110
Asn Val Leu Asn Gln Ser His Ser Gln Ser Leu Thr Pro Asn Ile Lys
115 120 125
Lys Thr Lys Glu Leu Ile Ile Tyr Tyr Asp Thr Tyr Arg Ile Asp Val
130 135 140
Asn Asn Met Thr Ile Asp Ile Thr Lys Lys Glu Phe Glu Ile Ile Lys
145 150 155 160
Leu Leu Ser Leu Asn Pro Gln Tyr Tyr Phe Ser Lys Glu Gln Ile Tyr
165 170 175
Asp Ser Ile Asn Leu Asn Lys Thr Gly Asn Thr Gln Val Val Ala Glu
180 185 190
His Ile Arg Lys Ile Arg Lys Lys Leu Ser Cys Tyr Ser Arg Phe Glu
195 200 205
Tyr Ile Asp Thr Lys Trp Gly Val Gly Tyr Lys Trp Leu Glu
210 215 220
<210>19
<211>251
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>19
Met Phe Lys Val Val Ile Cys Asp Asp Glu Arg Ile Ile Arg Gln Gly
1 5 10 15
Leu Lys Gln Met Ile Pro Trp Lys Glu Tyr His Phe Thr Thr Ile Tyr
20 25 30
Thr Ala Thr Asp Gly Val Glu Ala Leu Ser Leu Ile Arg Gln His Gln
35 40 45
Pro Glu Leu Val Ile Thr Asp Ile Arg Met Pro Arg Lys Asn Gly Val
50 55 60
Asp Leu Leu Asp Asp Ile Lys Asp Leu Asp Cys Gln Val Ile Ile Leu
65 70 75 80
Ser Ser Tyr Asp Asp Phe Glu Tyr Met Lys Ala Gly Ile Gln His His
85 90 95
Val Leu Asp Tyr Leu Leu Lys Pro Val Asp His Thr Gln Leu Glu His
100 105 110
Ile Leu Asp Ile Leu Val Gln Arg Leu Leu Glu Arg Pro His Ser Thr
115 120 125
Asn Asp Asp Ala Ala Tyr Tyr Thr Ala Phe Gln Pro Leu Leu Lys Ile
130 135 140
Asp Tyr Asp Asp Tyr Tyr Val Asn Gln Ile Leu Ser Gln Ile Lys Gln
145 150 155 160
His Tyr His Lys Lys Val Thr Val Leu Asp Leu Ile Asn Pro Ile Val
165 170 175
Val Ser Glu Ser Tyr Ala Met Arg Thr Phe Lys Glu His Val Gly Ile
180 185 190
Thr Ile Val Asp Tyr Leu Asn Arg Tyr Arg Ile Leu Lys Ser Leu His
195 200 205
Leu Leu Asp Gln His Tyr Lys His Tyr Glu Ile Ala Glu Lys Val Gly
210 215 220
Phe Ser Glu Tyr Lys Met Phe Cys Tyr His Phe Lys Lys Tyr Leu His
225 230 235 240
Met Ser Pro Ser Asp Tyr Asn Lys Leu Ser Lys
245 250
<210>20
<211>224
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>20
Met Gln Ile Leu Leu Val Glu Asp Asp Asn Thr Leu Phe Gln Glu Leu
1 5 10 15
Lys Lys Glu Leu Glu Gln Trp Asp Phe Asn Val Val Gly Val Glu Asp
20 25 30
Phe Ser His Val Met Glu Thr Phe Glu Thr Phe Asn Pro Glu Ile Val
35 40 45
Ile Leu Asp Val Gln Leu Pro Lys Tyr Asp Gly Phe Tyr Trp Cys Arg
50 55 60
Lys Met Arg Gln Gln Ser Asn Val Pro Ile Leu Phe Leu Ser Ser Arg
65 70 75 80
Asp Asn Pro Met Asp Gln Val Met Ser Met Glu Leu Gly Ala Asp Asp
85 90 95
Tyr Met Gln Lys Pro Phe Tyr Thr Asn Val Leu Ile Ala Lys Leu Gln
100 105 110
Ala Ile Tyr Arg Arg Val Tyr Glu Phe Gly Val Glu Glu Lys Arg Thr
115 120 125
Leu Ser Trp Gln Asp Ala Thr Val Asp Leu Ser Lys Asp Ser Ile Gln
130 135 140
Lys Asp Asp Lys Thr Ile Phe Leu Ser Lys Thr Glu Met Ile Ile Leu
145 150 155 160
Glu Met Leu Ile Asn Lys Arg Asn Gln Ile Val Thr Arg Asp Thr Leu
165 170 175
Ile Thr Ala Leu Trp Asp Asp Glu Ala Phe Val Ser Asp Asn Thr Leu
180 185 190
Thr Val Asn Val Asn Arg Leu Arg Lys Lys Leu Ser Glu Ile Asp Met
195 200 205
Asp Ser Ala Ile Glu Thr Lys Val Gly Lys Gly Tyr Leu Ala His Glu
210 215 220
<210>21
<211>229
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>21
Met Thr His Leu Leu Ile Val Asp Asp Glu Lys Asp Ile Val Asp Ile
1 5 10 15
Cys Gln Thr Tyr Phe Glu Tyr Glu Gly Tyr Gln Val Thr Thr Thr Thr
20 25 30
Cys Gly Lys Glu Ala Leu Lys Leu Leu Ser Ser Asp Ile Asp Ile Met
35 40 45
Ile Leu Asp Ile Met Met Pro Glu Val Ser Gly Tyr Asp Ile Val Lys
50 55 60
Lys Met Lys Asp Met Gln Leu Asp Ile Pro Phe Ile Tyr Leu Thr Ala
65 70 75 80
Lys Thr Gln Glu His Asp Thr Ile Tyr Ala Leu Thr Leu Gly Ala Asp
85 90 95
Asp Tyr Ile Lys Lys Pro Phe Ser Pro Arg Glu Leu Val Leu Arg Thr
100 105 110
Asn Asn Leu Leu Ala Arg Met Ser Lys Ser Asn His Ser Asn Lys Ile
115 120 125
Glu Gln Leu Glu Phe Asp Gly Leu Val Leu Lys Asn Leu Ser Lys Thr
130 135 140
Leu Thr Ile Asn Asn Ile Glu Ile Pro Met Arg Ile Lys Glu Phe Glu
145 150 155 160
Leu Leu Trp Tyr Leu Ala Ser Arg Glu Gly Glu Val Ile Ser Lys Ser
165 170 175
Glu Leu Leu Glu Lys Val Trp Gly Tyr Asp Tyr Tyr Glu Asp Ala Asn
180 185 190
Thr Val Asn Val His Ile His Arg Ile Arg Glu Lys Leu Glu Lys His
195 200 205
Asp Phe Leu Pro Tyr Thr Ile Thr Thr Val Trp Gly Leu Gly Tyr Lys
210 215 220
Phe Glu Arg Ser Arg
225
<210>22
<211>200
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>22
Met Ile Ser Leu Val Ile Thr Glu Asp Gln Leu Met Leu Arg Lys Ala
1 5 10 15
Met Val Gln Leu Ile Glu Met His Glu Glu Met Asn Val Ile Lys Asp
20 25 30
Phe Glu Asn Gly Val Glu Ala Leu Glu Phe Ile Glu Asn Asn Glu Pro
35 40 45
Asn Val Ala Ile Leu Asp Ile Glu Ile Pro Gly Leu Thr Gly Leu Glu
50 55 60
Ile Leu Ser Tyr Val Arg Lys Lys Gln Leu Asn Thr Arg Ile Ile Ile
65 70 75 80
Val Thr Thr Phe Lys Arg Pro Gly Tyr Phe Glu Lys Ala Val Ala Asn
85 90 95
Asp Val Asp Ala Tyr Val Leu Lys Glu Arg Ser Ile Asp Asp Leu Ile
100 105 110
Lys Thr Ile Tyr Lys Val Thr Lys Gly Glu Lys Glu Tyr Ser Pro Ser
115 120 125
Leu Met Thr Ser Leu Phe Thr Glu Ser Asn Pro Leu Thr His Lys Glu
130 135 140
Gln Val Val Leu Arg Glu Ile Gly Asn Gly Leu Ser Ser Lys Glu Ile
145 150 155 160
Ala Ser Lys Leu Tyr Leu Ser Asn Gly Thr Val Arg Asn Tyr Thr Ser
165 170 175
Thr Ile Ile Asp Lys Met Asn Ser Asp His Arg Phe Glu Ala Trp Lys
180 185 190
Lys Ala Asn Glu Lys Gly Trp Ile
195 200
<210>23
<211>219
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>23
Met Thr Asn Ile Leu Ile Val Glu Asp Glu Gln Asn Leu Ala Arg Phe
1 5 10 15
Ile Glu Leu Glu Leu Thr His Glu Asn Tyr Thr Val Asp Ile Glu Asn
20 25 30
Asp Gly Lys Val Gly Leu Asp Lys Ala Leu Ser Lys Pro Tyr Asp Leu
35 40 45
Tyr Ile Leu Asp Leu Met Leu Pro Asn Ile Asn Gly Leu Glu Ile Cys
50 55 60
Arg Gln Ile Arg Gln Lys Thr Thr Thr Pro Ile Ile Ile Ile Thr Ala
65 70 75 80
Lys Ser Glu Thr Tyr Asp Lys Val Ala Gly Leu Asp Tyr Gly Ala Asp
85 90 95
Asp Tyr Ile Val Lys Pro Phe Asp Ile Glu Glu Leu Leu Ala Arg Ile
100 105 110
Arg Ala Val Leu Arg Arg Gln Pro Asp Lys Asp Val Leu Asp Ile Asn
115 120 125
Gly Ile Ile Ile Asp Lys Asp Ala Phe Lys Val Thr Val Asn Gly His
130 135 140
Gln Leu Glu Leu Thr Lys Thr Glu Tyr Asp Leu Leu Tyr Val Leu Ala
145 150 155 160
Glu Asn Arg Asn His Val Met Gln Arg Glu Gln Ile Leu Asp His Val
165 170 175
Trp Gly Tyr Asn Ser Glu Val Glu Thr Asn Val Val Asp Val Tyr Ile
180 185 190
Arg Tyr Leu Arg Asn Lys Leu Lys Pro Phe Asn Lys Glu Lys Ser Ile
195 200 205
Glu Thr Val Arg Gly Val Gly Tyr Val Ile Arg
210 215
<210>24
<211>241
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>24
Met Thr Asn Glu Ile Leu Ile Val Asp Asp Glu Asp Arg Ile Arg Arg
1 5 10 15
Leu Leu Lys Leu Tyr Leu Glu Arg Glu Ser Phe Glu Ile His Glu Ala
20 25 30
Arg Asp Gly Lys Glu Ala Tyr Glu Leu Ala Leu Glu Asn Asn Tyr Ala
35 40 45
Cys Ile Leu Leu Asp Leu Met Leu Pro Glu Met Asp Gly Ile Glu Val
50 55 60
Ala Ser Lys Leu Arg Glu His Lys Asp Thr Pro Ile Ile Met Leu Thr
65 70 75 80
Ala Lys Gly Glu Glu Thr Asn Arg Val Glu Gly Phe Glu Ser Gly Ala
85 90 95
Asp Asp Tyr Ile Val Lys Pro Phe Ser Pro Arg Glu Val Val Leu Arg
100 105 110
Val Lys Ala Leu Leu Arg Arg Thr Gln Thr Ala Asn Thr Glu Gln Ser
115 120 125
Glu Pro His Ala Arg Asp Ile Ile Glu Phe Ser His Leu Val Ile Asp
130 135 140
Asn Asp Ala His Arg Val Leu Ala Asp Asp Gln Gln Val Asn Leu Thr
145 150 155 160
Pro Lys Glu Tyr Glu Leu Leu Ile Tyr Leu Ala Lys Thr Pro Asn Lys
165 170 175
Val Phe Asp Arg Glu Gln Leu Leu Lys Glu Val Trp His Tyr Glu Phe
180 185 190
Tyr Gly Asp Leu Arg Thr Val Asp Thr His Val Lys Arg Leu Arg Glu
195 200 205
Lys Leu Asn Arg Val Ser Ser Asp Ala Ala Gln Met Ile Gln Thr Val
210 215 220
Trp Gly Val Gly Tyr Lys Phe Glu Val Lys Pro Ser Asp Glu Pro Thr
225 230 235 240
Lys
<210>25
<211>236
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>25
Met Ser Gln Lys Ile Leu Val Val Asp Asp Glu Gln Ser Ile Val Thr
1 5 10 15
Leu Leu Lys Tyr Asn Leu Glu Thr Ala Gly Tyr Ile Val Glu Val Ala
20 25 30
Tyr Asp Gly Glu Glu Ala Leu Lys Lys Val Glu Thr Glu Gln Pro Glu
35 40 45
Leu Ile Val Leu Asp Val Met Leu Pro Lys Lys Asp Gly Ile Glu Val
50 55 60
Cys Lys Thr Ile Arg Ser Asp Lys Asn Leu Val Pro Ile Leu Met Leu
65 70 75 80
Thr Ala Lys Asp Asp Glu Phe Asp Arg Val Leu Gly Leu Glu Leu Gly
85 90 95
Ala Asp Asp Tyr Met Thr Lys Pro Phe Ser Pro Arg Glu Val Val Ala
100 105 110
Arg Val Lys Ala Ile Leu Arg Arg Ser Gln Phe Val Asn Glu Ile Glu
115 120 125
Lys Glu Asp Val Asp Asp Glu Asp Ile Ile Ile Gly Ser Ile Arg Ile
130 135 140
Arg Pro Glu Phe Phe Glu Val Tyr Lys Glu Asp Glu Leu Leu Glu Leu
145 150 155 160
Thr Pro Lys Glu Phe Glu Leu Leu Leu Tyr Leu Ile Glu Arg Gln Gly
165 170 175
Arg Val Ile Thr Arg Glu His Met Leu Asn Ser Val Trp Asn Tyr Glu
180 185 190
Phe Ala Gly Asp Ser Arg Ile Val Asp Val His Ile Ser His Leu Arg
195 200 205
Asp Lys Leu Glu Glu Asn Pro Lys Gln Pro Lys Leu Ile Lys Thr Val
210 215 220
Arg Gly Leu Gly Tyr Lys Leu Glu Arg Pro Lys Ala
225 230 235
<210>26
<211>207
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>26
Met His Lys Val Ile Leu Val Asp Asp His Tyr Ile Val Arg Gln Gly
1 5 10 15
Leu Glu Phe Leu Leu Ser Thr Val Asp Asp Leu Ser Val Leu Lys Gly
20 25 30
Phe Lys Asp Gly Ala Thr Phe Leu Ser Tyr Leu Lys Asn Asn Glu Arg
35 40 45
Pro Asp Ile Val Leu Leu Asp Leu Val Met Pro Glu Met Asn Gly Ile
50 55 60
Glu Ile Thr Glu Ile Leu Lys Gln Gln Tyr Pro Glu Ile Lys Val Leu
65 70 75 80
Val Leu Thr Ser Tyr Val Asp Asp Glu His Val Ile Ser Ala Ile Asp
85 90 95
Lys Gly Ala Asp Gly Tyr Glu Met Lys Asp Val Glu Pro Gln Lys Leu
100 105 110
Ile Glu Thr Ile His Lys Val Leu Gln Gly Glu Arg Ile Ile His Pro
115 120 125
Gln Ala Lys Ser Val Ile Lys Ala Val Ser Lys Lys Pro His Tyr Thr
130 135 140
Asn Lys Leu Ser Lys Arg Glu Lys Glu Val Leu Lys Glu Met Val Lys
145 150 155 160
Gly Lys Thr Asn Lys Glu Ile Ala Arg Ala Leu Phe Val Ser Glu Lys
165 170 175
Thr Val Lys Thr His Val Ser His Ile Phe Ser Lys Leu Glu Val Ser
180 185 190
Asp Arg Thr Gln Ala Ala Ile Tyr Ala Met Glu Asn Lys Leu Ile
195 200 205
<210>27
<211>209
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>27
Met Ala Ile Lys Val Leu Phe Val Asp Asp His Glu Met Val Arg Ile
1 5 10 15
Gly Ile Ser Ser Tyr Leu Ser Thr Gln Glu Asp Ile Glu Val Val Gly
20 25 30
Glu Gly Ala Ser Gly Lys Asp Ala Ile Thr Lys Ala His Glu Leu Lys
35 40 45
Pro Asp Leu Ile Leu Met Asp Leu Leu Met Asp Asp Met Asp Gly Val
50 55 60
Glu Ala Thr Thr Glu Ile Lys Lys Asp Leu Pro Gln Ile Lys Val Val
65 70 75 80
Met Leu Thr Ser Phe Ile Glu Asp Lys Glu Val Tyr Arg Ala Leu Asp
85 90 95
Ser Gly Val Asp Ser Tyr Ile Leu Lys Thr Thr Ser Ala Ser Asp Ile
100 105 110
Ala Asp Ala Val Arg Lys Thr Tyr Glu Gly Glu Ser Val Phe Glu Pro
115 120 125
Glu Val Leu Val Lys Met Arg Asn Arg Met Lys Lys Arg Ala Glu Leu
130 135 140
Tyr Glu Met Leu Thr Glu Arg Glu Met Glu Ile Leu Leu Leu Ile Ala
145 150 155 160
Lys Gly Tyr Ser Asn Gln Glu Ile Ala Ser Ala Ser His Ile Thr Ile
165 170 175
Lys Thr Val Lys Thr His Val Ser Asn Ile Leu Ser Lys Leu Glu Val
180 185 190
Gln Asp Arg Thr Gln Ala Val Ile Tyr Ala Phe Gln His Asn Leu Ile
195 200 205
Gln
<210>28
<211>238
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>28
Met Lys Ile Phe Val Cys Glu Asp Asp Gln Arg Gln Arg Glu His Met
1 5 10 15
Val Ser Ile Ile Lys Asn Tyr Ile Met Ile Glu Glu Lys Pro Met Glu
20 25 30
Leu Ala Leu Ala Thr Asn Asp Pro Tyr Glu Val Leu Glu Gln Ser Lys
35 40 45
Glu Leu Asn Asp Ile Gly Cys Tyr Phe Leu Asp Ile Gln Leu Glu Ala
50 55 60
Asp Met Asn Gly Ile Lys Leu Ala Ser Glu Ile Arg Lys His Asp Pro
65 70 75 80
Val Gly Asn Ile Ile Phe Val Thr Ser His Ser Glu Leu Thr Tyr Leu
85 90 95
Thr Phe Val Tyr Lys Val Ala Ala Met Asp Phe Ile Phe Lys Asp Asp
100 105 110
Pro Ser Glu Leu Lys Met Arg Ile Ile Asp Cys Leu Glu Thr Ala His
115 120 125
Thr Arg Leu Lys Leu Leu Ser Lys Glu Ser Asn Val Asp Thr Ile Glu
130 135 140
Leu Lys Arg Gly Ser Asn Ser Val Tyr Val Gln Tyr Asp Asp Ile Met
145 150 155 160
Phe Phe Glu Ser Ser Thr Lys Ser His Arg Leu Ile Ala His Leu Asp
165 170 175
Asn Arg Gln Ile Glu Phe Tyr Gly Asn Leu Lys Glu Leu Ala Gln Leu
180 185 190
Asp Glu Arg Phe Phe Arg Cys His Asn Ser Phe Val Ile Asn Arg His
195 200 205
Asn Ile Glu Ser Ile Asp Ser Lys Glu Arg Ile Val Tyr Phe Lys Asn
210 215 220
Gly Glu Asn Cys Phe Ala Ser Val Arg Asn Val Lys Lys Ile
225 230 235
<210>29
<211>224
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>29
Met Ile Asn Cys Leu Ile Val Asp Asp Asp Lys Lys Leu Leu Gln Tyr
1 5 10 15
Val Ser Ser His Leu Glu Arg Glu Ser Ile Gln Thr His Thr Phe Thr
20 25 30
Ser Gly Glu Ala Ser Leu Asp Phe Leu Glu Asn Lys Asn Val Asp Ile
35 40 45
Ala Ile Val Asp Ile Met Met Ser Gly Met Asp Gly Phe Glu Leu Cys
50 55 60
Gln Thr Leu Lys Asp Asp Tyr His Ile Pro Val Ile Met Leu Thr Ala
65 70 75 80
Arg Asp Ala Leu Ser Asp Lys Glu Arg Ala Phe Leu Ser Gly Thr Asp
85 90 95
Asp Tyr Val Thr Lys Pro Phe Glu Val Lys Glu Leu Leu Phe Arg Ile
100 105 110
Lys Ala Val Leu Arg Arg Tyr Gln Ile Asn Ala Asp Asn Glu Leu Gln
115 120 125
Leu Gly Asn Leu Ile Leu Asn Gln Ser Tyr Met Glu Ile Thr Val Gly
130 135 140
Ser Lys Thr Met Asn Leu Pro Asn Lys Glu Phe Gln Leu Leu Phe Leu
145 150 155 160
Leu Ala Ser Asn Pro Lys His Ile Phe Thr Arg Asp Asp Ile Ile Gly
165 170 175
Lys Ile Trp Gly Phe Asp Tyr Glu Gly Asp Asp Arg Thr Val Asp Val
180 185 190
His Ile Lys Arg Leu Arg Gln Arg Leu Ser Lys Leu Lys Ser Ser Val
195 200 205
Ser Ile Gln Thr Val Arg Gly Gln Gly Tyr Arg Val Asp Gln Asn Val
210 215 220
<210>30
<211>218
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>30
Met Lys Ile Val Ile Ala Asp Asp His Ala Val Val Arg Thr Gly Phe
1 5 10 15
Ser Met Ile Leu Asn Tyr Gln Glu Asp Met Glu Val Val Ala Thr Ala
20 25 30
Ala Asp Gly Val Glu Ala Tyr Gln Lys Val Leu Glu His Arg Pro Asp
35 40 45
Val Leu Ile Leu Asp Leu Ser Met Pro Pro Gly Glu Ser Gly Leu Ile
50 55 60
Ala Thr Ser Lys Ile Ser Glu Ser Phe Pro Glu Thr Lys Ile Leu Ile
65 70 75 80
Leu Thr Met Phe Asp Asp Glu Glu Tyr Leu Phe His Met Leu Lys Ser
85 90 95
Gly Ala Lys Gly Tyr Ile Leu Lys Asn Ser Pro Asp Glu Gln Leu Ile
100 105 110
Leu Ala Val Arg Thr Val Tyr Gln Gly Glu Thr Tyr Val Asp Met Lys
115 120 125
Leu Thr Thr Ser Leu Val Asn Glu Phe Val Asn Gln Ser Gln Thr Asp
130 135 140
Glu Val Ser Ser Ser Ser Asp Pro Phe Lys Ile Leu Ser Lys Arg Glu
145 150 155 160
Leu Glu Ile Leu Pro Leu Ile Ala Lys Gly Tyr Gly Asn Lys Asp Ile
165 170 175
Ala Glu Lys Leu Phe Val Ser Val Lys Thr Val Glu Ala His Lys Thr
180 185 190
His Ile Met Thr Lys Leu Asn Leu Lys Ser Lys Pro Glu Leu Val Glu
195 200 205
Tyr Ala Leu Lys Lys Lys Leu Leu Glu Phe
210 215
<210>3l
<211>252
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>31
Met Lys Ala Leu Ile Val Asp Asp Glu Pro Leu Ala Arg Asn Glu Leu
1 5 10 15
Gln Tyr Leu Leu Asn Leu Asn Asp Ala Ile Asp Glu Ile Glu Glu Ala
20 25 30
Glu Asn Ile Glu Glu Thr Leu Glu Lys Leu Leu Tyr Asn Thr Phe Asp
35 40 45
Leu Ile Phe Leu Asp Ile Asn Leu Met Asp Glu Ser Gly Ile Asp Leu
50 55 60
Ala Gln Lys Ile Asn Lys Met Lys Arg Ser Pro His Ile Ile Phe Ala
65 70 75 80
Thr Ala His Glu Lys Phe Ala Val Lys Ala Phe Glu Leu Asn Ala Thr
85 90 95
Asp Tyr Ile Leu Lys Pro Phe Glu Lys Glu Arg Ile Asn Gln Ala Val
100 105 110
Asn Lys Val Asp Met Ala Lys Asp Lys Ser Lys Asn Lys Asp Lys Thr
115 120 125
Ile Thr Pro Lys Tyr Ile Asp Tyr Ser Asp Asp Glu Arg Ala Gln Thr
130 135 140
His Val Leu Pro Ile Glu Val Asp Glu Arg Ile His Ile Leu Asn Phe
145 150 155 160
Thr Asp Ile Ile Ala Leu Ser Val Asn Asn Gly Ile Thr Thr Ile Asp
165 170 175
Thr Thr Lys Gln Ser Tyr Glu Thr Thr Glu Thr Leu Asn His Tyr Glu
180 185 190
Lys Lys Leu Pro Ser Ser Leu Phe Ile Lys Ile His Arg Ala Thr Ile
195 200 205
Val Asn Lys Glu His Ile Gln Thr Ile Glu His Trp Phe Asn Tyr Thr
210 215 220
Tyr Gln Leu Thr Leu Thr His Glu Phe Lys Tyr Gln Val Ser Arg Ser
225 230 235 240
Tyr Met Lys Thr Phe Lys Gln Gln Leu Gly Leu Gln
245 250
<210>32
<211>221
<212>PRT
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>32
Met Lys Ile Phe Ile Val Glu Asp Asp Leu Val Ile Ala Glu Ser Leu
1 5 10 15
Ala Asn Glu Leu Ser Lys Trp Asn Tyr Glu Val His Val Val Asp Asn
20 25 30
Phe Glu Lys Ile Met Glu Asp Phe Arg Arg Val Glu Pro Gln Leu Val
35 40 45
Leu Leu Asp Ile Asn Leu Pro Thr Leu Asn Gly Phe His Trp Cys Gln
50 55 60
Glu Met Arg Lys Val Ser His Val Pro Ile Met Phe Ile Ser Ser Arg
65 70 75 80
Thr Asp Asn Met Asp Gln Ile Leu Ala Ile Gln Met Gly Gly Asp Asp
85 90 95
Phe Ile Glu Lys Pro Phe Asn Leu Ser Leu Thr Val Ala Lys Ile Gln
100 105 110
Ala Leu Leu Arg Arg Thr Tyr Asp Leu Ser Ile Ala Arg Asp Glu Ile
115 120 125
Ala Val Lys Gly Cys Lys Leu Ile Val Asp Glu Ala Lys Leu Ser Lys
130 135 140
Asp Asn Glu His Val Gln Leu Ser Leu Thr Glu Leu Gln Ile Leu Lys
145 150 155 160
Leu Leu Phe Gln Asn Glu Asn Lys Tyr Val Ser Arg Thr Ala Leu Ile
165 170 175
Glu Lys Cys Trp Glu Ser Glu Asn Phe Ile Asp Asp Asn Thr Leu Ala
180 185 190
Val Asn Met Thr Arg Leu Arg Lys Lys Leu Leu Ser Ile Gly Val Asp
195 200 205
Asp Leu Ile Glu Thr Lys Lys Asn Val Gly Tyr Arg Val
210 215 220
<210>33
<211>1833
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>33
atgaagtggc ttaaacaact acaatccctt cacacgaaac tcgttattgt ttatgtacta 60
ctcattatta ttggtatgca aatcatcggt ttgtatttta cgaatagttt agaaaaggaa 120
ttactcgata acttcaagaa gaacataaca caatatgcga agcaattaga cgtcaatatt 180
gaaaaggtat ataaagataa agataaaggt tcagtcaacg ctcaaaagga tatccaagac 240
cttttgaatg aatatgcgaa tcgtcaagaa ataggagaaa tacgctttat tgataaagac 300
caaatcatca tggcaacaac caagcagtct aaccgtggtc ttatcaatca aaaggttaac 360
gacggttctg ttcaaaaggc gctctcctta gggcaaacga atgatcatat ggttcttaag 420
gattacggaa gtggtaaaga gcgtgtttgg gtatataata taccggttaa agtcgataaa 480
cagacaatcg gtgatatata catagaatcg aaaattaatg atgtatacaa tcagctgaac 540
aacattaatc agatattcat cgtagggaca gcgatatcac tattcattac agtgatacta 600
ggattcttca ttgcacgaac gattactaag ccgataaccg atatgcgtaa ccaaaccgtc 660
gagatgtcta aaggtaacta cacgcaacga gtgaagatat acggtaacga tgaaatcggt 720
gaactcgcac ttgccttcaa taacttatcg aaacgtgtcc aagaagcaca agcgaataca 780
gaaagtgaga aacgtcgtct agattccgtt atcacacata tgagcgatgg tattcttgcg 840
acagatcgcc gtggacgtgt acgtattgca aacgacatgg cgctgaaaat gctcggtctc 900
gcgaaagaag atgtcatcgg ctactacatg cttggtgtcc ttaacttaga aaatgaattc 960
tcattagaag aaattcaaga aaatagtgat tccttcttgt tagacattaa cgaagaagaa 1020
ggcattattg cacgtgtaaa ctttagtacg attgtacaag aaacaggttt cgtgacaggt 1080
tacattgccg tactacatga tgtcacagaa caacaacaag tcgaacgtga acgtcgcgaa 1140
ttcgttgcga atgtttcaca cgaattacgt acaccgctga catctatgaa tagttatatc 1200
gaagcacttg aagaaggtgc ttggcaagat aaagaattgg caccatcatt cctatctgtc 1260
acacgcgaag agactgaacg tatgattcgt ttagtgaatg atttacttca attatctaaa 1320
atggataatg aatcagatca aattacgaaa gaaattatcg acttcaatat gtttatcaac 1380
aaaattatta accgtcatga aatggcagcg aaagatacga cattcgtgcg cgaaattccg 1440
caacaaacca tctttgctga aatcgatcca gacaagatga cacaagtatt tgataatgtc 1500
attaccaatg caatgaaata ttcacgtggc gagaaacgtg ttgagtttca tgtgaaacaa 1560
aatgcacttt acaatagaat gacgattcgt attaaagata atggtattgg aataccgatt 1620
aacaaggtag ataaaatatt tgatagattc tatcgtgtag ataaagcacg tacacgtaag 1680
atgggtggta caggacttgg tctagctatt tccaaagaga ttgtcgaagc acataacggt 1740
cgaatttggg ctaacagtgt agaaggacaa ggtacgtcaa tctttattac gcttccttgc 1800
gaaatcattg aagacggtga ttgggatgaa taa 1833
<210>34
<211>1092
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>34
atggctagag tagaaagttt aaaaagaaaa atgacacgtc ttatcatagc aattatagtt 60
gtcagtgttg gattagtatt tttactacaa attttgctta atgtattaga attttcaaat 120
tttggaaagt ctttatacac taatttttgg aaacaaccac attacatttt ctcttttact 180
agcgcatgta tcagtattat gactcctttg ttaatcattt acgtgttcac acaatttttt 240
tataaaaagt acattacaac tagtattaat tatcttaatg atacgattca aggattaaaa 300
agtaaccgta ctgatgttga atataagaaa atgagtattt cagaatttaa taaattgatt 360
caagaagtag aacatataca tacttatctt tctaatagta ttaaagatca attatctatt 420
caaaataatt ttaaaagaaa actcgaaatt ttagaacatg atataaaaac accgttaact 480
gtccttcaag gtcatattga attattaaga aagattaatc gttctaatct gtctcaggag 540
gaaatacatt ttaaaataaa caaggaagca gacattgcgt tacaatctat tgatagaata 600
aataatcaat tacaaataca tattaaaaac atcaatctat tgccaaacca ttcagataca 660
attcatgttg ataaggtaat aaaaatcatt aaagagagct ataatcaaca atacatatta 720
aaggagagag aactgattat tgatgttgat catgatttcc cttatcatac cttaagcatt 780
aacaatcaag ttttatacca tgtcatcgat aatctaatta ataacgcctt gaaatatgct 840
gaaaaacaaa tatctataaa gtttgaagtt ataaacgacc atttactaaa cttcaccata 900
accaatgatg gtagaatatt tactgaagat gaactgaatt ctgccaaaga atgggggaca 960
aaaggtaaag aatcaaatgg gtctggtatt ggtctgtatt ttgctaatat tgtgcttcaa 1020
caatataata gtgaacttat tttagaaaat gagaataagc atgcaaaagt aagtttcaat 1080
attcctgtct aa 1092
<210>35
<211>1539
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>35
atgaagacct ataagccgta ccgacatcaa ttaaggcgtt cgctatttgc ctcaacgatt 60
ttcccagtat ttatggtgat gattattggt ttaataagct tttatgctat ttatatatgg 120
gtcgaacatc gcaccattca tcagcatacc tatcaaactc aaaccgaatt acaacgtatc 180
gacaaacatt ttcatacgtt tgttacgcag caacaaaaac aatggcgtca tgttgattta 240
tcacatccag ctgatatcac aaagatgaaa cgccaactat taaaacaagt ccatcaacaa 300
cctgcgatat tgtattacga tttaaaaggt tcttcacaat ctttcacaaa caattatgaa 360
caattagaca caacaaagat gtatttaata tcaaaatatc gaattgattt taaagacgat 420
acttatatcc ttaaaatata tatgtcaagc acaccactac ttaaaaacat taagaaaaat 480
agtggacaat ctgcactcat tgttgattca tatgatactg ttttatatac aaatgacgac 540
cgattctcta tcggtcaaaa atatcaacca ccacagtttg ggtttatgaa cgagtcttta 600
aaactcaatt ctcatcatgc gcatcttatt atatataaag atattcatga aaccattgaa 660
gatggaattg cattactagt tgtcatgggt gttgttctta ttctgcttgt tatttttgga 720
tatataagcg ctgatagaat ggcaaagcgc caatctgaag atattgaagc gattgtccga 780
aaaattgatg atgctaaaaa tcgacatctt ggtagttacg aaccgttaaa aaaacatagt 840
gagttagagg aaataaataa ttatatctat gacttgtttg aatcaaatga gcaattaata 900
caatctattg aacagaccga acgtcgttta cgtgatatac aattaaaaga aattgagcga 960
caatttcaac cccatttctt attcaatacg atgcaaacga tacaatattt aattcctctt 1020
tcacccaaag tagcacaaac agtcatacaa caactatcac aaatgctacg ttattctcta 1080
cgcacagcat cgcacacagt caaattagca gaagaattaa gctacattga gcagtatgtt 1140
gccatacaaa atatccgctt cgatgatatg atacagcttt acatcgatgc tactgagggt 1200
gtacaacatc aaacaattgg taagatgatg cttcaaccac tcgtagaaaa tgccatcaag 1260
catggtcgtg atagtgaacc tttaaagata acaattcgta tcagacttac gaagcgcaaa 1320
ttacatattc tggttcatga taatggcatc ggtatgtctc catcacattt agaacacgtg 1380
cgccaatccc ttcatcacga tgtttttgat acgacacacc taggtttaaa tcatttacat 1440
aatagagcca tgattcaata tggaacatat gcacgtttgc acattttctc aagaagccag 1500
caagggacat taatgtgtta ccaaatacca cttgtctag 1539
<210>36
<211>1041
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>36
atgaataatt ttcgatggtt ttggtttttt ataaaatctc gtattaattg gatattgtgg 60
atattatttc tcaacattat tttattaggt gttgcctaca tagattatga gatttcagtt 120
gaaagtgtat tttatatagt gatattaaac gtaggattga gtatactttt cttattattt 180
acgtttgtta aagaagtccg tctatctaaa catttttatg aagataaaga aattgaagaa 240
attaagcata aagatttggc agaaacccca tttcaacaac aagtcataga ttatctgtat 300
cgtcatatag ccgctcaaaa agagaaagta gtcgaacaac aattacaaat taaaaatcat 360
gaacaaacaa ttacagagtt tgttcatgat ataaaaacac ctgttacggc tatgaaatta 420
ttgatagatc aagagaatga tgatcagcgt aagcgagcgt tattatttga atggtctcgt 480
attaatgaga tgttagataa gcaattatat ttaacaaggc ttgaaacaca tcatcgtgat 540
atgtattttg attatatttc attaaagaga atggttatag atgaaataca agttactcga 600
catatcagtc aggcaaaagg gataggtttt gaattagatt ttaaagacga acaaaaggtt 660
tatacagatg ttaaatggtg ccgtatgatg attaggcaag ttctatctaa ctctttgaaa 720
tatagtgata attctacaat aaatttaagt ggttataaca tagaaggaca cgttgtttta 780
aaaattaaag actacggtcg tggaattagt aaaagagatt taccacgtat atttgataga 840
ggatttactt ctacaacaga ccgcaacgat actgcgtctt ctggtatggg attatacctt 900
gtacaaagcg tgaaagaaca acttgggatt gaagttaaag ttgattcaat agtggggaaa 960
ggaacaacgt tttatttcat tttcccacaa caaaatgaaa tcattgagcg catgtctaaa 1020
gtgacaagat tgtcatttta a 1041
<210>37
<211>966
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>37
atgtggttta atggtcacat gacactcact ttagccatca ctactatgat aacaagctgt 60
ttaacacttt cgatttgtag tatttttatt aatcctttaa ttcagaagat taagcaattc 120
aatattaaga ctaagcaatt tataaatcac gaaaaattca ttgatgatga aacatttcag 180
tctcctagag aaattaaaga gttgaatgat tcttttaata aaatggcata tgaaatcaat 240
aatcaaatga atatgataaa aaacgagcaa caagaaaaaa ctgaaatcat acaaaacctt 300
gcacatgatt taaaaacacc tttagctggt attcgatcat attctgaagg tttacgggat 360
ggcgtcatta gtgatcctca agaagttcat gaagcatatg aaatattaat taaacaagca 420
aatcgtttat caattttatt tgatgacatt acacatgtga ttaatttaaa tactggacgt 480
tcttatcctt tggaattaat acagctcgat caattacttg ttaatatatt acaaccatac 540
gaacaacaca taaaacaaga aaatcgtaca cttgaagtta atttctgcac agatattgat 600
gcattctacc agtatcgacc tccaatcgaa cgtattttga ccaatttact agataatgca 660
ttaaaatttt ctaattctgg tagccgtatt gatattatta tttctgagtg taaagaaaac 720
gacgtcatta gtatttcaat aaaagatgag ggcataggta tcgttccaga acttcaatca 780
cgtatctttg aaagaacgtt tagagttgaa gattctcgaa atactaagac tggtggttcg 840
gggttaggat tatacattgc aaatgagtta gcacaacaaa ttgacgcctc tattacagtc 900
caaagtgatt tagacattgg aaccacgatg acacttacct taaaaaaatt tcaatttaaa 960
aagtaa 966
<210>38
<211>732
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>38
atggaatcaa gaagttgggg agcgagaata ggggaattaa gtactttaat atatttaata 60
tttccgattt tagctatatt tgttgacaaa cgtggtaact tattcactta tttaattgtt 120
tgtactattt ttataatcag ctatgtgacg atgattttat tttataaaca ccttagtgat 180
agtattttat attcattact ggttattcat tatttaggaa tcttttattt tgtctatagt 240
gtcaatccta tgaatagttt gtttttcttc tatagtgcat tcgccttacc ttttattttt 300
aatgttcgtg ttgtatcaaa agaatttata acctttttaa tagctatgat aagttgctta 360
atactaactt atatatttaa tccaacattt gtggttccat taagtgcatt ttatttggtt 420
atattaattg ttgctgtagg taattttaaa aatagagacg aacgaattat gaaagctaaa 480
cttgaagaaa agaataagta tattaatgta ctaatagctc aacaagagcg taatagaata 540
agtcaagatc ttcatgatac attaggacat gtatttgcta gtcttacttt gaagtctgaa 600
ttagctgtta aactcataga tactaatcca aaagaagcaa aaaatgaaat gcaggccata 660
aatcaacttt caacagaagc cttaaataaa gtcagactca ttattgatga tttgaagata 720
ccaatctttt ga 732
<210>39
<211>1371
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>39
atgattaagc gccaaaaatt aaaatataaa tggatgctta ttacgacgct cattaccttc 60
acgacaatat tacttttctg cttaattatt attttctttt taaaagatac tttacgaagt 120
agtgaaattg acgaagctga aagaagttca aatgatatcg ccaatctgtt ccattctaaa 180
tctttaagtg atatatctgc attggattta aatgcatcct tagaaaattt tcaagaaata 240
ttgatttatg atgataaggg taggaagtta attcaaacat caaatgataa tacacttgct 300
tatgataaca aaattgattt caaacatcct gaacgtatac atattcaaag aagccacggt 360
attaattact tagtaataac tgaacctata cgttcgaaag atttttctgg atacagtgta 420
ttagtccatt ctcttcaaaa ttatgataat ctcgttaaat cactttatat agttgcactt 480
gcttttggat taattgcaac cattattact gccggcgtga gttatatctt ttcttcacaa 540
attactaaac cgatagttac aatgtccaat aaaatgaatc aaattagaag agatggtttt 600
caaaataaac ttgaattaac tacaaattat gaagaaacag ataatttaat tgatactttt 660
aatgaaatga tgtatcaaat agaagaatct tttaatcagc aacgtcaatt tgtcgaggat 720
gcttcacacg aattaagaac gccactgcag attattcaag gtcatctaaa tttaatccaa 780
cgttggggga aaaaagatcc agcagttttg gaagaatctt tgaatatttc aattgaagaa 840
gtgaatcgaa taacaaaact tgtcgaagaa ctacttttac ttaccaaaga tagagtcaat 900
cataatgttt tggaatgtga aaatgtagac gtaaatagcg agattcaatc acgtgtgaag 960
tcactgcaac acctacatcc agattatact tttgaaacac atcttgctac taagcctatc 1020
caattaaaaa ttaaccgtca tcagtttgaa caactcttac tcatatttat tgataatgca 1080
atgaaatacg acactgaaca taagcacatt aaaattgtta ctcaactaaa aaataaaatg 1140
attatgattg atattactga tcatggtatg ggtataccaa aagctgactt agaatttatc 1200
tttgatagat tttatcgtgt agataaatca cgtgctcgta gtcaaggagg caatggatta 1260
ggactatcaa tagcggaaaa aattgtgcaa cttaacggtg gtatgattca ggtagaaagt 1320
gaactacaaa agtacacgac tttcaaaatc agttttccag tactaaacta a 1371
<210>40
<211>1770
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>40
gtgatgaacc gactaaatag tgttgttata aaactgtggt taactattat tttaatagta 60
gcgacagttt taattttatt aagtgtttca ttaatcactt ttatacaata ttactttacg 120
caagaaacag aaaatgcaat taaagaagat gccaaaagaa ttagttcact tgttgagaag 180
tctcataaca aaacattagc tatccaaaat agccaaacac tcattgatgg tgatggaccg 240
ggtggcctca ttattatgaa agacatgagc gagaaaccag actcatcgta taaagacact 300
aaaaaacaaa tgtttaatga aataaaaaag agtactaaat ttaaaaaagt gtttaaagaa 360
ggtgagtatg aaactcaaaa tattacaata aaaaataaag gtaattctca atcttatctt 420
ttgctaggat acccaatgaa agctcaaaaa ggtgctcaaa gtcattatag tggtgttttt 480
atatataaag atttgaaatc tatcgaagat acaaataatg ctattacaat tattatatta 540
ataactgcta ttatatttac tatagcaagc acaatatttg cattcttttt atctaataga 600
ataacgaaac ccttacgtca attaaaaaca caagcacaaa aagtttctga aggggattat 660
agtcaaattt caactgtcgc tactaaagat gaaataggtg atttatcacg tgcatttaac 720
aacatgaacg tagaaattca agaacatatc aaagcaattt cttcatctaa gaatataaga 780
gatacattat taaactctat ggtagaaggc gttctaggca ttaataatca acgtgaaatc 840
atattgtcga acaagatggc tgatgatatt atgcgtcaca ttgatgattt ttcaaaagaa 900
tctattgaac agcaaattga agcaacattt gaatcacaac agaatgagta tttagaatta 960
gaaattaata caaggtacta tgtatttatc tccagttata tagatagaat tcaaacaaat 1020
ggtagaagtg gtattgtcat ggtcatccgt gatatgacaa atgaacataa tcttgatcaa 1080
atgaaaaaag attttatagc aaatgtatca catgaattac gtacgccaat ctctttatta 1140
cagggttaca ctgagtccat agtagacggt atagttaccg aaccagatga aatacgtgac 1200
tcattagcaa tcgttttaga tgaatctaag cgacttaatc gtttagtcaa tgaattacta 1260
aatgtagctc gtatggatgc tgaaggatta tcagttgaga aggaattaca acctattcaa 1320
caccttcttg ataaaatgga gtctaaatat cgcatgcaaa gtgaagaatt aggtttaaca 1380
atgacgtttg attctaataa tgacgaacaa ttatggaact atgatatgga tagaatggac 1440
caagtgttaa ctaatttaat tgataacgca acaagatata cacaagctgg tgattctata 1500
aagatttcta ttgatgaaga ttcagatttc aatattttaa caataactga tacaggcact 1560
ggtatagcgc cggaacatct gaaacaagta tttgaccgtt tttataaagt ggacgctgct 1620
cgaaaaagag gtaagcaagg caccggatta ggacttttca tttgtaaaat gattattgaa 1680
gaacacgggg gacgtattga tgttgagagc gaattaggca aaggtacttc atttattatt 1740
agactaccta aatcaaaaca aattagttag 1770
<210>41
<211>1698
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>41
atgttgaagt tccatttgcg tttattacta ttaattagta ctattacaat cattagtttt 60
attggtttag gcgctatcat tcacaacaca atttatcaaa cattaactag taatcaaatt 120
aaaagcttag atagtgaagc ccgtaattat gttaatctct ttaataataa taaagagaaa 180
gaaatcacta atatagctca taatgaaaaa aatatcatat taataaaaga aaaagataaa 240
gataaaatta tttactcaag tggaaatatt aaagatattg accatagaat tgataatgaa 300
gctaatcctt cgaaattaat caataaaaac acaaaactcg gtatgcgtta cacctataag 360
aatacaatag atgataaaac aatatacata agtggaatta ataatgaaat tattgattta 420
caaaaggatt tatggaaata cttgtctatt gttggagtca ttgtattatt tacggtctat 480
ttagcaagta gaagtatcaa tcgaacatat attagaccta tcaatgaagt aacttatgct 540
acatcacttc tagcagatgg atattaccat gttcgtgttc cagaaagtaa tgtgaaggaa 600
actagggcat tatttgtgac tacaaatgac ttagcacgac gattgcaaaa attaaacaat 660
agtcaaaaaa ttcaatccaa tagattaaaa actaccttag aaaatatacc gagttcagta 720
ctgatgattg ataaacatgg agaaattgta gttgctaatc atgcttatta tcaggtgttt 780
aaccctgatc aaatggtaga aaataaaagt tacattggtt tcatagatga tagtattgaa 840
aaattaatta ttgaaagttt tagaactgaa aaagttatct atgaacaatt agaagttgct 900
attaataacg tacatactaa atatttcgat gtatcttgta tccccatttt aactaaatct 960
aaaaaaaatt tacaaggtat ggtggttgtg cttcatgaca ttactaattt gcagaaatta 1020
gaaaacctta gaagggaatt tgttgcaaat gtgtcacatg aactaaaaac accgattact 1080
tcaatcaaag gttttgcaga aactctgatt gaaggtgcta aaaatgatga acaatcgctt 1140
gatatgtttt taaatattat tttaaaagaa tctaatagaa tagagtcatt ggttacagac 1200
ttattagatt tatcacatat agaacagcaa aaagaacttg aaataaatta catgaattta 1260
tctgaattag ctattaatat aatagataat ttgcaaacac aagcatacaa taagagaatc 1320
aaaatacaat ctgaaattga aaaagatgtc atcattgagg cacatgaaaa taaaatagcg 1380
caagttatta ctaatttgct atcaaatgct ataaattatt cttcagaaga taataaggta 1440
atagtaagag tatatagaaa tgacaataaa gtttatttag agattcaaga ttatggtatt 1500
ggtataagtg aaacagatca aaagcgtata tttgaacgtt tctatcgtgt agataaagcg 1560
agaagtagag attcaggtgg tacaggactt ggtctgtcta taacaaaaca tattgttgaa 1620
gcacataatg gtagaataga cgtgaaaagt gcacctggca aaggttcgat attcaaagtt 1680
ctatttaatg ataattaa 1698
<210>42
<211>1134
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>42
atggagaaac caactagact tgcattactt aaggaaatag ccgaatttct aaatgaagaa 60
actgaaatat atagcatgat gcaaggagct ttgcgtttac ttataaatgg gagcgacttt 120
tcaactggtt ggattttcta tattgatgaa gaggggcacc atgatttagt ttcctctgta 180
gatttacctg aagctttatc taaaaaccat tgttactatt tgacaaatgg ttcttgttgg 240
tgtgtgcaag catatcaaaa tcataaatta actaaagctt ctaatataat aaattgttct 300
agaatcaatt tagctaatca tgcttatgat aaagaaacga atcatattac tcatcatgct 360
actgttccat tgcagtctgg gagcgaaaga ttcggtttgc ttaacgtagc atctcctaac 420
aaagaacatt atagtgaaga agacttagaa ctattagaat ctgtagcatt tcaaattggt 480
tcagctataa agagaattca gctaactaat aaagaaaaag aaaatgccaa aattaatgaa 540
cgtaatcgac tagctagaga cttacatgat tcagtaaatc aaatgttatt ttctttaaaa 600
ttaacggctc acgctgcaaa agggataact actgacgagc aagcgcaaag agcttttaag 660
actattgagg aaacaagtca aaatgcagta aatgaaatga gagcactgat ttggcaacta 720
aaaccagttg gattagaaca aggcttaatt catgcattac actattatag taaaatgtta 780
ggattagatt tgaaagttac tattaatgga ctaatagatt tatctaatga tatcgaagaa 840
aatgtataca gaattattca ggaagcaatg aataatgtta ttaagcacgc taaaactaac 900
aaagtttatc ttgatttatc acaaaatgtt gattatcttg aggtagatat tagagataac 960
ggtcaaggtt ttgacacgca atcaattaat acgtttttat ttaatggttt aaaaaacata 1020
aagcaacgta cacagtattt gaacggtaaa gttactatag aatcaaaaat aaatcagggc 1080
actcggattt atattaaaat tccattgaag ttaaaggagc atccttatgc ataa 1134
<210>43
<211>1047
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>43
atgaatcact acataagagc tataggctca atgcttatat tagtatatag tatgcttatt 60
gcttttttat ttattgataa agtgtttgta aatattatct tttttcaggg gatgttttat 120
acacaaatat ttggaatacc tgtttttcta tttttaaatt tattaattgt tcttttatgt 180
attatagttg gatctgtttt agcttataaa attaatcaac aaaatgattg gattatttca 240
caaatagaaa gatcaataga aggacaaaca gtaggtatca atgatcaaaa tatcgaatta 300
tatacagaaa cgatagatat ttatcataca ctagttccat taaatcaaga attacatcga 360
cttagaatga agactcaaaa tttaactaat gaaaactaca atattaatga tgtaaaagtc 420
aaaaagatta tcgaagatga gcgacaacga cttgccaggg aattacatga ttctgttagt 480
caacaattat ttgctgcgag catgatgcta tcggcgataa aagagtcgaa attagaacca 540
cctttaaatc aacagatacc aattcttgaa aaaatggttc aagactcaca acttgaaatg 600
agagctttgt tattacattt aagaccgata ggtttaaaag ataagtcttt aggtgaagga 660
attaaagatt tagtcatcga tttacaaaag aaagtaccaa tgaaagttgt gcatgaaatt 720
caagattttg aagtgccaaa aggcattgaa gatcacttgt tcagaattac acaagaagct 780
atttcaaata cattgagaca ttcaaatggt acaaaagtaa ctgtggaatt atttaatcaa 840
gaggattatc ttttactaag aattcaagat aatggaaaag ggtttaatgt agatgaaaaa 900
tttgaacaaa gttatggttt gaaaaatatg cgagaacgag cgttagaaat tggtgcgacg 960
tttcatattg tatctttacc tgattcaggt acacgaattg aagttaaggc accattgaat 1020
aaggaggaga attcaagtgg cgattaa 1047
<210>44
<211>1290
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>44
atggatgata ttaatctatt tccgtttgca ggcctacaaa tctttttaat gatttgggtt 60
actaaagtta tcattaatat gaaatttaat tttagggatt acataatcgt ttttacgatt 120
gtaatccctt ctgcaataat gtattacttt tggcaaagta aagcattaat agttttggtt 180
ataataatca ccattttctt ttatacaaaa ataaagcttt attcaatatt agttgtatta 240
ttcaccacta tgatcttata tataactaat ttcataactg tatacataca tttgactata 300
aaagattata ttccgtttaa atttgcttta cagttaatac attttacctc ttttgtaatc 360
ataactctaa ttattgctta tttaactcaa ctattgttca ataaattaaa agtatcctac 420
ttgtcactca ataaaagata cttattgata ataacaatag tacttttcat atcatttatt 480
ttactttata tggtgtcaca aactgatatg cgaggaaatg atacccttaa attatatgcc 540
atcttattgt tgggtattat ggttttttta agtgtagtga tattagtgat gtccaatttt 600
acactccgtg aaatgaggta taaacgtaat gtaaaagaaa tcgaagcata ttatgagtac 660
acgttacgta tagaaagcat taacaatgaa atgcgtaagt tccgacatga ttatgtgaat 720
atcctcacca ctctttcaga ttacattaga gaagatgata tgcctggatt acgtaaatat 780
tttaatgaaa atatcgttcc aatgaaagat aaattaaaaa ctcgctctat taaaatgaat 840
ggtattgaaa agttgaaagt gagagaaatt aaagggctga ttactactaa aattattcaa 900
gctcaagaaa aacgtattcc aattagtatt gaggttcctg atgaaattga tcgtatcgat 960
atgaatactg ttgagcttag tcgtattatc ggtattatag ttgataatgc tattgaagct 1020
tcagaaaatc ttgaggaacc actcatcaat atcgcattca tcgataatga ggaatctgtc 1080
acttttatcg ttatgaataa atgtagtaat gatatcccta aaattcatga gttgtttgaa 1140
caaggttttt ctactaaagg tgataatcgc ggtttaggtt tatcaacttt aaaagaactg 1200
acagactcaa acgagaatgt tttattagat actgtcatcg aaaatggtta ctttgtacaa 1260
aaagtagaaa taaataataa ggaatcataa 1290
<210>45
<211>1356
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>45
atgtttaaaa cattatattc gagaattgct atatatgcaa ttacagttat tttgtttagt 60
gcattaatga gttttctttt tacgaatatt tattatcact ttcatttgaa agcttctaat 120
gatgcaaaaa ttatgcgcac actcaaagaa gctcgtgaat atgaaagaac acaaaaacct 180
aaacctctag atacgtattt gaaacattta ggtcaaatga attatcaaat tatgactgtc 240
aacgaacatg gaaccaaaca tttttatggg gaaacttttc gtaaaaatac tataagtcag 300
tctgcaatca aaaaagtgct caacggtgaa gattatcatg gtattaaaaa taaaccttat 360
gcattttttg taacagggtt cttcgataat gaaacggata atacagtagg tattcaattt 420
aaaacagatg atggggcatt agcagttttt atgcgcccag atatcgggaa aacttttagc 480
gaatttagaa ttttcttagc tgtgttgata acacttttac ttattatttc aatttcacta 540
gtcattgcat caacatattc tattattaaa ccagtgaccg cattaaaaaa tgcaacaact 600
cgaattatga aaggtgattt cagtacgcct atcaaacaaa cacgtcacga tgaaatcgga 660
acactgcaat cacgtttcaa tacaatgcgt caaaatttag gacaagtaga tcaaatgaga 720
caacattttg tccaaaatgt atctcatgaa attaaaacac cgcttacaca tttacaacga 780
ttacttacac aacttgaatt gacgcaaaat gaagaagaga aacaactatg tatcaatgaa 840
atgtttgaaa tcactaatca agtaagtgaa ctaactaagg aattgctact tttatctgaa 900
ttagataatg cttcccacct tacttttaac gataatgttc atttaaatac tttaataaag 960
gatattatta gacatgaaca atttcgaaca gatgaaaaag atttagtttt gtttactgaa 1020
ttagaagatt tgtattttcg aggaaatgaa agactattac atcaagcttt caataacctc 1080
atcattaatg caatgaaata tgctcctcaa aatagcatga ttaatatcac tctaactagt 1140
acaaatcact tgattatatt taatattgaa aatgatggat cgattgcaga agaagatgcg 1200
aaacatatat tcgatcgttt ttataaactg agtgacgaat ctagtagtaa tggtctaggt 1260
ctagccatta cccaatcaat cattcatctt catcatggta gcattactct cacttcagat 1320
gataaaacac aatttattgt aaaactattt atttag 1356
<210>46
<211>1044
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>46
gtggtaaata tgttggagca aactgattta agtttagagc aattacttaa gaattattat 60
gaaaccacga acgagaaaat tgtatttgtt aatagacaag gcaaaattat tgctatgaat 120
gacgcagcaa aagatatttt aactgaggaa gataattata atgctatgac aaatgcgatt 180
tgtcatcgat gcgaaggata ctctaatgaa tatgatgtac aatcgtgtaa agattgtttt 240
ttagagacaa cgcaattaca acattccaat ttccaagtat ttatgaagac aaaagataat 300
gaaattaagc cttttacagc tatgtatcaa aatattgatg aacaaagagg tattagtgca 360
tttaccttac agaatgtggc gcctcagatt gaaaggcaag aaaaaatgta tcaacaaaaa 420
atgttacatc gttcaattca agcacaagaa aatgaacgaa agcgtatttc tagagaatta 480
catgatagtg taatacagga tatgctcaat atagatgttg aactaaggct tttgaagtat 540
aagcacaggg ataaggtgtt agctgaaaca tctcaacgta tagaaggact attatcacag 600
cttattgatg atattagaaa tatgtctgtt gaattaagac cttcttctct cgacgattta 660
ggtattgaag cagcttttaa atcatatttt aaacagtttg aagaaaatta tggtatgcat 720
attaaatatg attcgaatat taaaggcatg cgttttgata atgaaattga aacagttgtg 780
tatcgtgtag ttcaagaggg tgtatttaat gctctaaaat atgctgaggt taatgaaatt 840
gaggtaagta cgcatagtga tggcaagcag cttgtagcag aggttgtgga tcgaggtaaa 900
gggtttagtt tagatcatca ccctaaaggc tctggacttg gattgtacgg aatgagagaa 960
cgtgcagaat tagttaacgg tcatgttaat atagagacac atattaatag aggtactata 1020
attacattag atataccgat ttaa 1044
<210>47
<211>1791
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>47
atgaggattg acgttatgct taatttattt attttattac ttgaacgcgt gggattaatt 60
attttactcg catatatact catgaatatt aatcatttta aaacaatgat gagcgaacgt 120
gacaaatggc gttcaaaatt ccaactaatc attatttttg gtatattctc tatgatttca 180
aattttacag gaattgaaat agaaaatggc catatcgtat ctagtgatat ttattaccac 240
ttaagcaaag acgcctcaat ggccaatacc cgtgtattaa caatcggtgt ttctggtcta 300
ataggtggac cgtgggtagc tataattgta ggcattattt caggattatg tcgtttatat 360
attggcgggg ctgacgcata tacctatctt atttcatcca tagttatcgc tatcatatct 420
ggttactttg gtcatcaaac aataaaacaa aatacatatc cgtccattaa aaaaggtgct 480
attatcggtg caataactga aattattcaa atgggctgta tattattatt tacaaataac 540
ctacatcacg caattacatt agtcagtttt atagcactac cgatgattat cattaatagt 600
ttaggtacag ccatattttt aaccatcatt ttgtcgacca tcaagcaaga agaacaaatg 660
cgtgcagtcc agacgcatga tgtattacaa ctcgctaacg aaacactacc ttactttcga 720
tctggactta atgaaaaatc agcccaacaa gctgctgaaa ttattttaaa attaatgcaa 780
gtgtcggcgg tcgctattac aaataaaaaa gatattttaa cacatattgg tgcaggcagt 840
gaccatcacg tagcacgtaa agagataatt actgatttat ctaaagaagt gattcaatct 900
ggaaaattaa aagtcgctca tacgagagaa ggaattggat gtcaccatcc taattgccct 960
cttgaaggag ccattgtcgt ccctctttat atacataatg aagtcgcagg gactttaaaa 1020
ttttatttca cagataacaa tattatttca acttcagacc aacagctcgc aaaaggtctc 1080
gccaatatat tttcctcaca gctagaacta ggtcaagctg aaatgcaagg acaattattg 1140
aaagacgctg aaattaaatc attacaagca caagtcaatc cacatttctt ttttaatgct 1200
attaacacta tatcagcact agtaagaatt gatagcgaaa aagcacgacg tttactgatt 1260
caactcagtc agttcttccg ctctaatctt aatggtgcac gtaataatac gattacttta 1320
caaaaggaat tacaacaagt agcagcatat ttatccttag agcaagctcg ctatccaaat 1380
agatttaaca tacactatcg aattgatgat cagtgtcaag atgcgctcat cccccctttt 1440
ataattcaaa tattagtgga aaattctatt aaacatgcat ttaaaaatcg taaaaagaat 1500
aatcatattg atgtggatgt tagcatgaag caagactgct taagtatatc tgttcaagat 1560
aatggtcaag gcataccagc tgatcaatta gatactattg gatatacgac agtaacgtct 1620
accactggta ctggtaatgc cttagtcaat cttaataaaa gacttactgg actatttgga 1680
acaacatcgg cactgaacat tcaatcttct caatcaggca cgactgtaag ttgtttaatt 1740
ccatataaat cttctaagga ggaacacttt aatgaaagcg ttaatcgttg a 1791
<210>48
<211>897
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>48
atgaagtttg catatattca atcgattcgt aatgagattt caattatttt aataattcta 60
ttattttttg cacttatatt ttatgtgttt tctttacctt ttgatgcata cgtactagca 120
atcagtataa tattactatt gatgtgtgta cgttggtgga taaagtattt aagttttaaa 180
aagaatgaac atcttaaaga taaagtagca tatttagaac atgagttagc acatgttaag 240
aatcagcaaa ttgaatatcg taacgatgtt gaaagttatt ttttaacatg ggtacatcaa 300
attaaaacac ctatcactgc ctcacaatta cttttggaga gaaacgagga gaatgtagtt 360
aatcgtgttc gacaagaaat tgtgcacatt gataattata caagtctcgc attaagttat 420
ttaaaattat taaatgaaga gtcagatatg acaattacca aagtgacagt tgatgatttg 480
attcgcccgt tgattttaaa atatagaatt cagtttattg aacaaaagac gcaaatccat 540
tatgaaaaaa gtgaggacat tattttaacc gatgcacaat gggcttctat aatgatagag 600
caacttttaa ataatgcttt aaaatatgct aaaggtaaag atatttggat agattttgat 660
gttgccaatc aaactctaca gattaaagat aatggtattg ggattagtaa agcagatatt 720
cctaaaattt ttgataaagg atactcagga tttaacggta gattgaatga acaatcaact 780
ggtataggtc tatttatagt gcaacacatt gcaaatcatt taaatataca agtaactgta 840
caatcagagt tgaatcatgg gacagtattt tttatacatt ttactaaaga aaaataa 897
<210>49
<211>702
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>49
atggctagaa aagttgttgt agttgacgat gaaaaaccaa ttgctgatat tttagaattt 60
aatttaaaaa aagaaggtta cgacgtatat tgcgcttatg atggtaatga cgcagtagat 120
ttaatctatg aagaagaacc agatatcgtc ttacttgata tcatgttacc tggtagagat 180
ggtatggaag tatgtcgtga agtgcgtaaa aagtatgaaa tgccaattat catgttaaca 240
gcgaaagatt ctgaaattga taaagtatta ggccttgaat taggtgcaga tgattacgta 300
actaaaccat ttagtactcg tgaactcatc gcacgtgtga aagcgaactt acgccgtcat 360
tattcacaac cagctcaaga agtaagtggt gcgacaaatg aaattacaat taaagatatt 420
gtgatttatc cagatgcata ttcaattaaa aaacgtggag aagacattga attaacgcat 480
cgtgaattcg agctattcca ttatctttct aaacatatgg gtcaagtcat gacacgtgaa 540
cacttactac aaacagtgtg gggttacgat tatttcggtg atgttcgtac tgtggacgta 600
acaattcgcc gtttaagaga aaaaattgaa gatgatccat ctcatccaga atacattgtg 660
acacgtagag gcgttggata cttcctccaa caacatgatt ag 702
<210>50
<211>669
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>50
gtgatgtata acataatgat tgtcgacgat gaagaaaaaa ttgtgtcatt tatagaagaa 60
agtttatact tagaaggttt taacactatt gtcgcttata atggacgtga cgttttaaaa 120
caaatcgatg acaaaattga tttaatcatt ttagatatta aaatgccgta tatagatggg 180
tttgaagttg ctcaatcact taaagagagt aatataccta ttatttttct tactgctaaa 240
gataatcttg atactagact gaaatgtttc tcgcttggtg caaaggatta cttacctaag 300
cctttctata tggaagaact tataattcga attaaaaatg tacttaatca aagtcattct 360
caaagtttga ctcccaacat aaaaaaaact aaagaattaa tcatttatta cgatacatat 420
agaattgacg taaataacat gacgatagat attacaaaaa aagaatttga aattataaaa 480
ttactatctt taaatcctca atattatttt agtaaagaac agatatatga ttcgattaat 540
ttaaataaga caggcaatac tcaagttgtt gctgaacata tacgtaaaat aagaaaaaag 600
ttaagttgtt atagtcgttt tgaatacatc gatacaaagt ggggagttgg atataaatgg 660
ctagagtag 669
<210>51
<211>792
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>51
gtgttaccaa ataccacttg tctaggagga ataaagatgt ttaaagtagt tatttgtgat 60
gatgaacgga ttatacgaca aggtttaaaa caaatgattc catggaagga atatcatttt 120
acaactattt acactgccac agacggcgta gaagcattgt ctttaattcg ccaacatcaa 180
cctgaactcg tcattactga tatacgaatg cctcgaaaaa atggtgttga cctactagat 240
gacatcaaag accttgattg ccaagttatc attttatcta gttatgacga cttcgaatat 300
atgaaagccg gcattcaaca ccatgttctt gattatttac taaagccagt agaccacact 360
caattagagc atattctaga catattagtt caaaggttat tagaacgtcc acattctacc 420
aatgatgacg cggcatatta tactgccttt caaccattat taaaaattga ttacgatgac 480
tattatgtca atcaaatttt gtctcaaatc aagcaacatt atcacaagaa agtgactgtt 540
cttgacttaa ttaatcctat tgttgtaagt gagtcatacg ccatgaggac gtttaaagaa 600
catgtaggca ttacgatagt tgattattta aatcgttatc gtattttaaa atcattacat 660
cttttagacc agcactacaa gcattatgaa attgctgaaa aagtaggttt ttctgagtat 720
aagatgtttt gctatcattt taaaaaatat ttacatatgt caccaagtga ttataataag 780
ctatcaaaat ag 792
<210>52
<211>675
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>52
atgcaaatat tattagtcga agacgataat acgctttttc aagaattaaa aaaggaatta 60
gaacaatggg attttaatgt cgttggtgtc gaagatttta gtcatgtgat ggagacattt 120
gaaacattta atccagaaat agttattttg gatgttcaat taccgaaata cgatgggttt 180
tattggtgtc gaaaaatgag acaacagtcg aacgttccta ttttattttt atcatcaaga 240
gataatccaa tggatcaagt gatgagtatg gaacttggtg cagatgatta tatgcaaaaa 300
ccattttata caaacgtctt aattgctaag ctacaagcta tttatagacg cgtttatgaa 360
tttggagttg aagaaaagag aacgttaagt tggcaagacg ctactgtgga tttatcaaaa 420
gatagtattc aaaaagatga taaaactatc tttttgtcta aaacagagat gattatttta 480
gagatgttaa tcaataaacg taatcaaatc gtgacacgag acactctcat tactgctttg 540
tgggatgatg aagcttttgt tagtgataat actttaacag ttaatgttaa tagattaaga 600
aaaaaattat cagaaattga catggatagt gcaattgaaa ccaaagttgg taaaggatac 660
ttagctcatg aataa 675
<210>53
<211>702
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>53
gtgaagataa gcatgaccca cttactaatc gtggatgatg aaaaagatat tgtagacatt 60
tgtcaaacgt atttcgaata tgaaggatat caggttacga caacaacatg tggtaaagaa 120
gcactaaaat tattatcttc agatatagat attatgattt tagacattat gatgccggaa 180
gttagcggat acgatattgt caaaaaaatg aaagatatgc aacttgatat acctttcata 240
tatctaacag caaaaactca agaacatgac acgatatacg cactgacgtt aggtgctgat 300
gactatatta aaaaaccctt tagcccaaga gaactagtat tacgtactaa taaccttctc 360
gctagaatga gtaaatcaaa tcattctaat aaaattgagc aattagaatt tgatggttta 420
gttttaaaaa atttaagcaa gacacttacc attaacaata tagagatacc tatgcgcatt 480
aaagaatttg aattgctatg gtatcttgct tctagagaag gtgaagttat ttctaaatcc 540
gaacttctag aaaaagtatg gggatatgac tattacgaag atgctaacac tgtcaatgtc 600
cacatacata gaattcgcga gaaattagaa aaacatgatt ttttacccta cactattaca 660
actgtgtggg gccttggtta taagtttgaa aggagccgat aa 702
<210>54
<211>603
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>54
atgatatcgc ttgttataac tgaggatcaa ttgatgttaa gaaaagctat ggtacaactg 60
atagaaatgc atgaagaaat gaatgtaata aaagattttg aaaatggtgt agaggcactc 120
gaatttattg aaaataatga acccaatgta gccattttag atattgaaat tccgggttta 180
acaggcttgg aaatcttatc ttatgtaaga aaaaaacaac tcaatacaag aataatcata 240
gtgactacat ttaaacgtcc aggttatttt gaaaaagccg tagcaaatga tgttgatgct 300
tatgtactaa aagagcgttc aattgatgat ttaattaaaa ctatttataa agtgacaaag 360
ggtgagaagg aatatagccc ttcgctcatg acatcattgt ttactgaatc gaatccatta 420
actcataaag aacaagttgt tttaagagaa ataggcaatg ggttaagtag caaagagatt 480
gctagcaaat tgtatttatc gaacgggact gtgcgtaact atacgagtac tattatagac 540
aaaatgaatt cagatcatcg ttttgaggca tggaaaaaag caaatgaaaa ggggtggatt 600
taa 603
<210>55
<211>660
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>55
atgacaaata ttttaattgt agaagatgag caaaatcttg ctagatttat agagcttgag 60
ttaactcatg aaaattatac tgttgatata gagaatgatg gaaaggtggg tttggataaa 120
gcattatcta agccctatga tttatatata ttagacttaa tgcttccaaa cataaatggt 180
ctagaaattt gtagacaaat tcgtcaaaaa acaactactc caattatcat cattactgcg 240
aaaagcgaga catatgataa agtagctgga ttggactatg gggcagatga ctacattgta 300
aaaccctttg atatagaaga attgcttgca agaataagag cggtattgcg cagacagcca 360
gataaagatg ttttagatat caatggtatt atcattgata aagatgcctt taaagttact 420
gttaatggcc atcaattaga attaactaaa acagaatacg atttactata tgttttagct 480
gaaaatcgta accacgtcat gcaacgtgaa caaattctcg atcacgtatg ggggtataat 540
agtgaagtag aaacgaatgt cgttgatgtt tatattcgtt atttacgtaa taaactcaaa 600
ccttttaata aagaaaaatc catagaaaca gtacgtggcg tagggtatgt gattcgatga 660
<210>56
<211>726
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>56
atgactaacg aaattttaat cgttgatgat gaagatagaa ttagaagatt acttaaatta 60
tatttagaaa gagaatcttt tgaaattcat gaagcaagag atggtaaaga agcctacgag 120
cttgctcttg aaaacaacta tgcgtgcatc ctcttagatt taatgcttcc tgaaatggat 180
ggaattgaag ttgcctctaa acttagagaa cataaagata cacctatcat catgcttact 240
gcaaaaggtg aagaaacaaa tcgtgttgaa ggatttgaat cgggagcaga tgattatatt 300
gttaagcctt tctcacctag agaagtagta ttgagagtaa aggcgttatt aagacgtaca 360
caaactgcaa acactgagca aagcgaaccg catgcacgcg atattattga atttagtcat 420
ctagtgattg ataatgacgc tcatcgagtc cttgcggatg atcaacaagt gaatctaaca 480
cctaaagagt atgaattact catctattta gctaaaactc caaataaagt atttgatcga 540
gaacagttac ttaaagaggt ttggcattac gaattttatg gggacttgcg tacagtagac 600
actcacgtca aaagacttag agaaaagtta aatcgagtat caagtgatgc tgcacaaatg 660
atacaaactg tgtggggtgt cggttataaa tttgaggtaa aacctagtga tgaaccgact 720
aaatag 726
<210>57
<211>738
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>57
atgtataaaa agattaggag gcacactatg tcacaaaaaa tattagtggt tgatgatgag 60
caatccatcg tgaccttact aaaatataat ttagaaacag cagggtatat agtcgaagtc 120
gcttatgatg gtgaagaggc attgaaaaaa gttgaaacag aacaaccgga actaattgta 180
ttagacgtta tgttgccaaa aaaagatggt atagaagtat gtaaaacaat caggtctgat 240
aaaaatttag taccgatttt aatgttgaca gcaaaggacg atgaatttga tcgagtttta 300
gggcttgaac taggtgctga tgactatatg acaaaaccat tttcacctag agaggtagtt 360
gccagagtta aagcaatatt aagacgttct caatttgtaa atgaaattga gaaggaagat 420
gttgatgatg aagatattat cattggttca attagaattc gacctgaatt ctttgaagtc 480
tataaagaag atgaattatt agaattgacg ccaaaagaat ttgaattgtt attatattta 540
attgaaagac aaggaagagt tattactcga gaacatatgt tgaattcagt gtggaattat 600
gaattcgctg gagattctcg aattgttgat gtacatataa gccacttaag agataaattg 660
gaagaaaatc ctaagcaacc taaattgata aagactgtac gtggtttagg ttataaactg 720
gagagaccta aagcttaa 738
<210>58
<211>624
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>58
atgcataaag tcattctcgt cgatgatcac tatattgtaa ggcaaggttt agagttttta 60
ttatcaactg tagatgattt atcagtttta aagggtttta aagatggcgc aacattttta 120
tcttatttaa aaaacaatga aagaccggac atcgtgcttt tagacttagt aatgccagag 180
atgaatggta tagaaattac tgaaatactc aaacaacaat atcctgaaat caaagtatta 240
gtacttacga gctatgtaga tgatgaacat gttatttcag ctattgataa aggagctgat 300
gggtatgaaa tgaaagatgt tgaacctcaa aagttaattg agacaattca taaagttttg 360
caaggtgaac gtattataca tcctcaagct aaaagcgtga ttaaggccgt atcaaaaaaa 420
ccgcattata cgaataagtt atctaaacgt gaaaaagaag tgcttaagga aatggttaaa 480
ggtaaaacaa ataaagaaat tgcaagagca ttgtttgtat cagaaaagac agtaaaaaca 540
catgtgagtc atatttttag caaactagaa gtctccgata gaactcaagc cgctatttat 600
gcaatggaaa ataaattgat ttaa 624
<210>59
<211>630
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>59
gtggcgatta aagttttatt tgttgatgat catgaaatgg taagaatcgg tatttcaagc 60
tatctttcaa ctcaagaaga tatagaagta gtaggtgagg gtgcttctgg taaagatgct 120
atcactaaag cgcatgagct aaaaccagat ttaatattaa tggatttact tatggacgat 180
atggatggtg tagaagcaac tactgaaata aaaaaagatt tacctcaaat taaagtagtc 240
atgttaacaa gctttataga ggataaagaa gtttatcgtg cacttgattc tggagtagat 300
agttatattt taaagacaac aagtgcaagt gatatagctg acgctgtgcg taaaacgtat 360
gaaggtgaat cagtatttga accagaagtg ttagtaaaaa tgcgcaatcg tatgaaaaaa 420
cgtgccgagc tttatgaaat gttgacagaa agagaaatgg agatcctatt acttatagct 480
aaaggatact ctaaccaaga gattgcaagc gcctctcata tcaccatcaa aacagtaaaa 540
actcatgtaa gtaacatact aagtaaatta gaagtacaag atcgaacaca agcagtaata 600
tatgcgttcc agcataattt aattcaataa 630
<210>60
<211>717
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>60
atgaaaattt ttgtttgtga agatgaccaa agacaaagag aacatatggt atcaatcatt 60
aaaaactaca taatgattga agaaaagcca atggagttag ctttagcaac aaatgatcct 120
tatgaggtct tagagcaatc aaaagaactt aatgacattg gttgttactt ccttgatatt 180
caattagaag ctgatatgaa cggtattaaa ttagccagtg aaattcgtaa acatgatcct 240
gttggtaata ttatatttgt aaccagtcac agtgagctga cttatttgac gtttgtttat 300
aaagtggctg ctatggattt tatttttaaa gatgatccat ctgaattaaa aatgagaatc 360
atagattgtc ttgaaacagc acatacacga ctcaaattat tatcaaaaga aagtaatgta 420
gatacgattg agttaaagcg gggaagtaat tcagtatacg ttcaatatga tgatattatg 480
ttttttgaat catctacgaa atctcataga ctcattgcac atcttgataa tcgacaaatt 540
gaattttatg gaaatttaaa ggaattagca cagcttgatg aacgtttctt tagatgtcat 600
aacagttttg taataaacag gcataatatt gaatctattg actcaaaaga acgtattgtt 660
tactttaaga atggcgaaaa ttgtttcgct tcagtacgta atgttaaaaa aatataa 717
<210>61
<211>675
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>61
atgattaatt gcttaatcgt agacgatgat aaaaagttat tgcaatatgt ttcaagtcat 60
ttagaaagag aaagtattca aacacatact ttcacaagtg gagaagcatc actagatttt 120
cttgaaaata aaaatgttga tattgcgata gtagatatta tgatgagtgg aatggatggt 180
tttgagcttt gtcagacttt gaaagatgat tatcatattc ctgtcataat gttgacagct 240
agagatgcat taagtgataa agaacgtgca tttctaagtg ggactgacga ttatgtcact 300
aaaccttttg aggttaaaga attattattt agaattaaag ctgtcttaag acgatatcaa 360
attaatgctg ataacgagtt acaacttggc aacttaatat taaatcagtc ttatatggaa 420
attactgtgg gttcaaaaac gatgaatctt ccaaacaaag aatttcagtt gttattttta 480
ttagcctcta atcctaaaca tattttcact cgagatgata ttattggaaa aatttggggc 540
ttcgattatg aaggagatga tcgtactgtt gatgttcata ttaaaagatt acgtcaacgt 600
ttatctaaat tgaaatcatc agtatcaatt caaactgtaa gaggtcaagg atatagggtg 660
gaccaaaatg tttaa 675
<210>62
<211>657
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>62
gtgaaaatag ttatagcgga tgaccatgca gttgttagga caggattttc aatgatatta 60
aattatcaag aagatatgga agttgttgca actgcagctg acggggttga agcttatcaa 120
aaagtgttag aacatcgacc agatgtttta attttagatt tgagcatgcc accaggagag 180
tctggcttaa tcgcaaccag taaaatttct gaaagttttc ctgaaactaa aattttaata 240
cttacgatgt ttgatgacga agaatattta tttcatatgt taaaaagtgg tgctaaagga 300
tacattttaa agaattcacc tgatgagcaa ttaatattgg ccgtacgtac agtatatcaa 360
ggtgaaactt atgttgatat gaaattgacg acgtctttag tcaatgagtt tgtcaatcaa 420
tctcaaacgg atgaagtgtc atcctcttca gatccattta aaattttatc gaaacgagag 480
ttagaaatat tacctcttat agctaaaggc tatggcaata aagatattgc agaaaagttg 540
tttgtatcag tgaaaacggt agaggcacat aaaacgcata ttatgacgaa actaaattta 600
aagagtaaac ctgaattagt tgaatatgcc ttaaagaaaa aattattaga attttaa 657
<210>63
<211>759
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>63
atgaaagcgt taatcgttga tgatgaacca cttgcaagaa atgaattaca atatctttta 60
aatcttaatg atgctataga tgaaattgaa gaagcagaaa acatagaaga aacattggag 120
aagttactct acaacacatt tgacttaata ttcttagata taaatttaat ggatgaaagt 180
ggtattgatt tagctcaaaa aattaataaa atgaagcgat caccacatat tatctttgca 240
accgctcacg agaaatttgc agtcaaagcc tttgaattaa atgcaaccga ttatatatta 300
aaaccttttg aaaaagaacg tattaatcaa gctgtaaata aggttgacat ggctaaagat 360
aaatcaaaaa acaaagataa aactatcaca cctaaatata ttgattatag tgatgatgag 420
cgcgctcaaa cacatgtact cccaattgaa gtggatgaac gtattcacat cttaaatttc 480
acagacatta tcgcattatc tgttaataat gggattacaa cgatagatac aacaaaacaa 540
agttatgaaa cgaccgaaac acttaatcat tacgagaaaa aactaccttc ctctctattt 600
attaaaatac atcgcgctac tatcgttaat aaagaacata tccaaacaat agagcattgg 660
tttaattata cgtatcagct gacgttaaca catgaattta aatatcaagt tagtcgttct 720
tatatgaaga cttttaaaca acaacttggt cttcaataa 759
<210>64
<211>666
<212>DNA
<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>64
atgaagattt tcattgtaga agacgattta gtgattgctg aaagtttagc taatgagcta 60
tctaaatgga attatgaggt gcatgtggtt gataattttg aaaagataat ggaagatttt 120
cgccgagtag aaccacaact cgtattatta gatatcaatc taccaacact taatggcttc 180
cattggtgcc aagaaatgcg aaaagtatct catgtgccta ttatgtttat tagttcaaga 240
acagataata tggatcaaat tctggctatc caaatgggtg gcgatgattt tatcgagaaa 300
ccatttaact tgtctttaac agtagctaaa attcaggcac tattgcgtcg cacttatgat 360
ttatcaatag ctcgtgatga aatagctgtt aaaggttgta aactaattgt tgatgaggcc 420
aagcttagca aggataatga acatgttcaa ctttccttaa cagagctaca gatattaaaa 480
ttgttatttc aaaatgaaaa taaatatgta agtcgtacag ctttaattga gaagtgttgg 540
gagtctgaga attttattga tgataatacg ctcgcagtaa atatgacacg attacgaaaa 600
aaattacttt ctatcggtgt cgatgattta attgagacaa agaaaaatgt aggatacagg 660
gtttga 666