毒力基因和蛋白质及其用途 本申请是国际申请日为1999年11月9日，中国申请号为99814336.7发明创造名称同上的申请的分案申请。
【发明领域】
本发明涉及毒力基因和蛋白质的鉴定及其用途。更具体地说，本发明涉及它们在治疗和筛选药物中的用途。
【发明背景】
大肠杆菌属于肠杆菌科或肠道细菌，它们是聚居在动物肠道中的革兰氏阴性微生物。该细菌科中的其它细菌包括肠杆菌属、克雷伯氏菌属、沙门氏菌属、志贺氏菌属和耶尔森氏菌属。尽管通常在人胃肠道中发现大肠杆菌，但是它与包括败血病、脑膜炎、尿道感染、伤口感染、脓肿形成、腹膜炎和胆管炎在内的人体疾病有关。
由大肠杆菌导致的疾病取决于某些毒力决定簇。例如，大肠杆菌与新生儿脑膜炎有关并已将主要的毒力决定簇鉴定为K1抗原，它是唾液酸的均聚物。K1抗原可以在回避宿主免疫系统和防止吞噬作用方面起作用。
发明概要
本发明以一系列大肠杆菌K1中的毒力基因的鉴定以及相关的生物体为基础，其产物可能与生物体的致病性有关。
本发明的一个方面是一种由操纵子编码的肽、或其在革兰氏阴性菌中的同源物或其功能片段，其中所述的操纵子包括来自大肠杆菌K1的本文鉴定为mdoG、creC、recG、yggN、tatA、tatB、tatC、tatE、eck1、iroD、iroC、iroE、mtd2和ms1-16在内的任意基因。例如，这类肽在分离时适合于治疗用途。
本文所用的术语“功能片段”指的是保留与完整基因或肽类似的治疗用途的一部分基因或肽。例如，可以将所述肽地功能片段用作在疫苗或生产抗体过程中有用的抗原决定簇。可以将基因片段用于编码活性肽。另一方面，该基因片段可能在基因疗法中有用，从而靶向体内的野生型基因以便发挥治疗作用。
本发明的肽可以包括本文鉴定为SEQ ID NOS.2、5、7、9、11、12、13、14、16、23、24、25、26、28、31、29、32和35-48的任意氨基酸序列。
将这些肽类鉴定为毒力决定簇使得能够将它们以许多方式用于治疗感染。例如，可以将宿主转化以表达本发明的肽或将所述宿主进行修饰以便破坏编码所述肽的基因的表达。一种疫苗还可以包括用于治疗感染的本发明的肽或用于其表达的工具。此外，疫苗可以包括具有毒力基因缺失的微生物，其中所述的基因编码本发明的肽。
根据本发明的另一个方面，可以将所述肽类或基因用于筛选有潜能的抗微生物药或用于检测毒性。
本发明的另一个方面是本文所鉴定的任意产物在治疗或预防与革兰氏阴性菌、特别是大肠杆菌导致的感染有关的疾病中的用途。
发明描述
本发明利用了特征标记的诱变(STM)(Hensel等《科学》(Science)1995；269：400-403)以便筛选减毒突变体的大肠杆菌K1菌株RS228(Pluschke等《感染与免疫》(Infection and Immunity)39：599-608)小Tn5突变体库，从而鉴定了大肠杆菌的毒力基因(和毒力决定簇)。
尽管将大肠杆菌K1用作鉴定毒力基因的微生物，但是也将其它肠道细菌中的相应基因看作属于本发明的范围。例如，以序列同源性为基础，可以在肠杆菌属、克雷伯氏菌属和包括沙门氏菌属、志贺氏菌属和耶尔森氏菌属在内的与人体肠道疾病相关的其它属中发现相应的基因或编码的蛋白质。
本文所用的术语“毒力决定簇”指的是一种产物，例如一种可能起维持病原菌作用的肽或蛋白质。特别地，毒力决定簇是一种与感染性或导致疾病的微生物有关的细菌蛋白质或肽。
可以将编码毒力决定簇的基因称作“毒力基因”。通过突变、缺失或插入破坏毒力基因会导致细菌在宿主中的存活率降低或通常导致微生物致病性的降低。
已经证实特征标记的诱变是一种用于鉴定毒力基因及其产物的极为有用的技术。该项技术依赖于转座子在允许条件下随机插入微生物基因组中的能力。将转座子分别标记以便于鉴定且然后分别引入微生物，从而使基因组受到破坏。接着通过阴性选择来检测毒力降低的突变型微生物并对已经发生插入失活的基因进行鉴定和特征记述。
STM过程中的第一个阶段是制备合适的转座子或转座子样元件。制备不同转座子文库，将它们各自引入载体或质粒以促进其转入微生物。本领域技术人员显然可以制备带有合适转座子的载体且这项技术进一步公开在WO-A-96/17951中。对于革兰氏阴性菌例如大肠杆菌来说，合适的转座子包括Tn5和Tn10。在制备了转座子后，则对细菌菌株进行诱变以便建立各突变型细菌的文库。
接着将收集的突变型微生物引入合适的宿主。在适当长的时间后，从宿主中回收微生物并鉴定已经在宿主中存活的那些微生物，由此还鉴定了不能存活的突变型菌株即减毒株。然后将储存文库中相应的减毒株用于鉴定发生插入失活的基因。通常，通过分离位于转座子插入位点侧翼的DNA来鉴定转座子插入的位点且这使得对与毒力相关的基因进行特征记述成为可能。
一旦鉴定了无毒性微生物，则能够通过用致死剂量的突变体感染合适的宿主动物而更充分地确定突变型基因在毒力中的潜在作用。将受感染动物的存活时间与用野生型菌株感染的对照组动物的存活时间进行比较且一般可以认为存活期限比对照组动物长的那些动物感染了带有突变型毒力基因的微生物。
另一方面，通过用野生型和突变体细菌的混合物感染动物宿主可以研究毒力中的潜在作用。在合适的时间期限后，从宿主动物的器官中收集细菌并测定野生型和突变体细菌的比例。将该比例除以接种物中突变体与野生型细菌的比例来确定竞争指数(CI)。可以认为具有竞争指数小于1的突变体是无毒性的。
有可能通过插入转座子而失活的基因可能不是真正的毒力基因，但是可以对下游(毒力)基因具有极性作用。这可以通过进一步的实验来确定，所述的实验过程包括使非极性突变置于基因的更为确定的区域或使其它邻近基因发生突变并确定该突变体是否是无毒性的。
由于对大肠杆菌中的毒力基因进行了特征记述，所以有可能使用用来确定其它微生物中同源性的基因序列。以这种方式，有可能确定其它微生物是否具有相似的毒力决定簇。通过检索例如EMBL或Genbank这样的现存的数据库可以确定序列同源性。
通常使毒力基因在称作致病性岛的不同染色体区中彼此群集。当致病性岛通常位于重复序列、插入元件或tRNA基因侧翼时，可以识别它们。另外，G+C含量一般不同于染色体的剩余物，这提示它们通过从另一种生物体中的水平传递而获得。例如，大肠杆菌K12的G+C含量为52％。在大肠杆菌菌株中发现的任意致病性岛可能具有不同于该平均值的G+C含量。
经鉴定的毒力基因能够用于生产减毒的疫苗菌株和作为抗微生物药的靶物。一般来说，这对于革兰氏阴性菌中的同源物来说同样确切。
为了本发明的目的，同源性的适当程度一般至少为30％，优选至少为50％、60％或70％，且更优选至少为80％或90％(在氨基酸或核苷酸水平上)。
通过本领域中已知的方法可以纯化和分离本发明的蛋白质。特别地，由于鉴定了基因序列，所以有可能使用重组技术在合适的宿主中表达基因。可以鉴定活性片段和同源物并可以将它们用于治疗中。例如，可以将蛋白质或其活性片段用作疫苗中的抗原决定簇以便引起免疫反应。还可以将它们用于制备被动免疫用抗体或用于诊断应用。合适的抗体包括单克隆抗体或包括单链fv片段在内的其片段。抗体的制备方法对于本领域技术人员来说是显而易见的。
以减毒微生物为基础的疫苗制品对本领域技术人员来说是已知的。如果必要或需要，可以用例如明矾这样的合适的载体或佐剂来配制疫苗组合物并可以将它们用于治疗，从而提供对大肠杆菌或其它革兰氏阴性菌的有效免疫接种。疫苗制品的制备方法对于本领域技术人员来说是显而易见的。
更为常见的和正如本领域技术人员众所周知的，当可以含有合适的载体或赋形剂时，可以对本发明活性成分的治疗用合适量和给药途径进行选择。根据诸如所治疗疾病的性质/严重程度、患者的类型或健康情况等这样的已知标准来选择或决定这些因素。
下列实施例解释了本发明。对于这些实施例来说，使用全身感染的小鼠模型将STM用于筛选减毒突变体的大肠杆菌K1小Tn5突变体库。该实验方法的基本步骤公开在Hensel等的上述文献中。未从接种了混合型突变体群体的小鼠中回收到毒力基因内含有小Tn5插入物的大肠杆菌K1且因此该大肠杆菌K1可能是被减毒的。
通过反向PCR或通过拯救卡那霉素抗性标记克隆了位于小Tn5插入物各侧翼的DNA区。在后一种情况下，将来自STM衍生的突变体的染色体DNA用限制酶消化、连入质粒pUC19并在转化入感受态大肠杆菌K12细胞后选择卡那霉素抗性克隆。然后进行随后的克隆和测序步骤并使用可公开得到的序列数据库(EMBL)中的序列与所述基因序列进行比较以便辅助进行对推定基因产物的特征记述。
实施例1
在第一种突变体中，对所克隆的DNA的两个片段进行测序。将核苷酸序列表示为SEQ ID NO.1和SEQ ID NO.3并将来自SEQ ID NO.1的DNA翻译区表示为SEQ ID NO.2。SEQ ID NO.1显示出与来自大肠杆菌K12(EMBL数据库登记号AE000206)的mdoGH区的2577-6908位核苷酸具有99.8％的同一性。这种DNA片段编码ymdD基因的5’部分、完整的mdoG基因和mdoH基因的5’部分。mdoG基因产物的功能是未知的，不过，认为它与膜衍生的寡糖类的生物合成有关。
SEQ ID NO.3显示出与来自大肠杆菌K12的mdoGH基因的3’部分和下游基因序列(7187-7760位核苷酸)具有98.3％的同一性。SEQID NO.2显示出与来自大肠杆菌K12(Swiss Prot登记号P33136)的mdoG蛋白质在1-511位氨基酸上具有99.6％的同一性。
在全身感染的小鼠模型体内测试新型基因对使用混合型感染的毒力的减毒作用(Achtman等《感染与免疫》(Infection and Immunity)1983；第39卷：315-335)并证实以0.38的竞争指数被减毒。这证实原始转座子突变体的减毒可能是因mdoG基因受到破坏而导致的。
构建mdoG的极性和非极性缺失突变体。通过使用寡核苷酸5’-TGCTCTAGAGCCATTACTCAGAATGGG-3’(SEQ ID NO.49)和5’-CGCGAGCTCGACGACTGAATGATCCC-3’(SEQ ID NO.50)进行PCR来扩增mdoG基因和侧翼区。将产物克隆pUC19。然后通过使用寡核苷酸5’-TCCCCCGGGTACTGCAGCACTCAACC-3’(SEQ ID NO.51)和5’-GATCCCGGGACCACTGAAATGCGTGC-3’(SEQ ID NO.52)进行反向PCR来扩增含有mdoG的5’-和3’-末端片段和完整pUC19序列的PCR产物。将非极性卡那霉素抗性弹夹(aphT)沿两个方向插入mdoG序列之间而得到极性和非极性构建体。接着将mdoG∷aphT融合体转入自杀载体pCDV442。通过将pCDV442构建体连入野生型大肠杆菌K1后的等位转移使mdoG的染色体拷贝发生突变。
测试所构建的突变体在全身感染小鼠模型体内对毒力的减毒作用(Achtman等，文献同上)。分别以0.37和0.35的竞争指数(平均值C1来自三只小鼠)使极性和非极性构建体的毒力得到弱化。这证明原始转座子突变体的减毒可能是因mdoG基因受到破坏而导致的。
实施例2
用具有以SEQ ID NO.4表示的核苷酸序列和表示为SEQ ID NO.5的翻译的氨基酸序列的毒力基因鉴定第二种突变体。小Tn5转座子插在581位核苷酸(SEQ ID NO.4)和187位氨基酸(SEQ ID NO.5)上。
这些序列显示出与大肠杆菌K12(EMBL和Genbank登记号M13608、AE000510和U14003)的creC基因具有97.9％的同一性。
来自大肠杆菌K12的creC蛋白质属于组氨酸激酶的蛋白质族且属于由含信号结构域的蛋白质组成的蛋白质族。
测试新型基因对毒力的减毒作用(Achtman等，文献同上)且证实该新型基因是以0.09的竞争指数被减毒的。
当将大肠杆菌K12 creC基因转录成带有creD基因的操纵子的一部分时，这种减毒作用可能是因对推定大肠杆菌K1 creD基因的极性作用所导致的。
实施例3
第三种突变体在紧接小Tn5后带有表示为SEQ ID NO.6的核苷酸序列。将翻译的该序列表示为SEQ ID NO.7。
该核苷酸序列显示出与大肠杆菌K12(EMBL和Genbank登记号P24230和M64367)recG基因的5-146位核苷酸具有93.7％的同一性。这表明受到破坏的基因至少部分与大肠杆菌K12的recG基因相同。大肠杆菌K12的recG基因编码起ATP依赖性DNA解旋酶作用的76.4kD蛋白质并在DNA修复过程中起关键作用。
在减毒试验中，证实竞争指数为0.48。将recG基因转录成操纵子的末端基因且因此由于对另一种大肠杆菌K1基因产生极性作用而导致这种减毒作用是不可能的。
实施例4
如果翻译产物表示为SEQ ID NO.9，那么第四种突变体带有在表示为SEQ ID NO.8的核苷酸序列内插入的转座子。
小Tn5转座子插在359位核苷酸和80位氨基酸上。
这些序列显示出与大肠杆菌K12(EMBL登记号AE000378)的yggN基因的339-1054位核苷酸具有98.5％的序列同一性且在氨基酸水平上具有99.6％的同一性。
尽管yggN基因的序列是已知的，但是其编码的蛋白质的功能尚未确定。
测试新型基因对毒力的减毒作用且证实该新型基因是0.43的竞争指数被减毒的。
实施例5
通过在相同区内插入转座子还发现了几种突变体。对该区的克隆和测序显示了表示为SEQ ID NO.10的核苷酸序列。该序列与大肠杆菌K12(EMBL和Genbank登记号AJ005830、AE000459和AE000167)的tatABCD操纵子具有同源性。该操纵子编码推定质量为9.6kD、18.4kD、28.9kD和29.5kD的蛋白质，它们起Sec依赖性蛋白质输出途径中成分的作用。在细胞质中结合辅因子后，该途径使全折叠的蛋白质通过门控孔移位至周质。
对核苷酸序列的翻译显示了相当于tatA的蛋白质(SEQ IDNO.11)、相当于tatB的序列(SEQ ID NO.12)、相当于tatC的序列(SEQ ID NO.13)和相当于tatD的序列(SEQ ID NO.14)。
由STM鉴定的突变体中的小Tn5转座子定位于SEQ ID NO.10的1429和2226位核苷酸上。这些转座子的插入破坏了tatB蛋白质序列的50位氨基酸和tatC蛋白质序列的143位氨基酸。
测试tatB和tatC基因对毒力的减毒作用且证实这些基因分别以0.0012和0.0039的竞争指数被减毒。当在全身感染的相同模型中测试单一感染时，这些基因在毒性上也发生了弱化。
实施例6
在相当于表示为SEQ ID NO.15的大肠杆菌K12的tatE基因的区内通过插入方式使另一种突变体失活。该序列的翻译如SEQ ID NO.16所示。tatE基因显示出与大肠杆菌K12基因(登记号AE000167)的6719-7306位核苷酸具有98％的同一性。
为了确定tatA、tatD和tatE基因是否需要毒性，在它们各自中构建非极性缺失突变。使用下列引物扩增位于各tatA、tatD和tatE基因侧翼的DNA区：
tatA
5’-TCG TCT AGA GAT GAT GGT GAT GGA GCG-3’(SEQ ID NO.53)
5’-GAA CTG CAG CCA AAT ACT GAT ACC ACC C-3’(SEQ ID NO.54)
5’-GAA CTG CAG GCT AAA ACA GAA GAC GCG-3’(SEQ ID NO.55)
5’-CAT GCA TGC ACT CCA TAT GAC AAC CGC-3’(SEQ ID NO.56)
将引物SEQ ID NO.53和SEQ ID NO.54用于扩增tatA上游的DNA序列；将引物SEQ ID NO.55和SEQ ID NO.56用于扩增tatA下游的DNA序列。
tatD
5’-TCG TCT AGA ATG AAG CTG CGC ATG AGG-3’(SEQ ID NO.57)
5’-CAA CTG CAG TCG CAA ATT GCG AAC TGG-3’(SEQ ID NO.58)
5’-CAA CTG CAG ACC GCA ACT TTT CGA CGC-3’(SEQ ID NO.59)
5’-CAT GCA TGC CAG TGA GCC ATT GTT CCC-3’(SEQ ID NO.60)
将引物SEQ ID NO.57和SEQ ID NO.58用于扩增tatD上游的DNA序列；将引物SEQ ID NO.59和SEQ ID NO.60用于扩增tatD下游的DNA序列。
tatE
5’-TGC TCT AGA TAC GAC TCT GAC AGG AGG-3’(SEQ ID NO.61)
5’-TCA GAT ATC AAC TAC CAG CAG TTT GG-3’(SEQ ID NO.62)
5’-TCA GAT ATC CAT AAA GAG TGA CGT GGC-3’(SEQ ID NO.63)
5’-TGC TCT AGA AAA CGT GGC AAC AGA GCG-3’(SEQ ID NO.64)
将引物SEQ ID NO.61和SEQ ID NO.62用于扩增tatE上游的DNA序列；将引物SEQ ID NO.63和SEQ ID NO.64用于扩增tatE下游的DNA序列。
在将这些侧翼DNA片段克隆入pUC19中后，将非极性aphT卡那霉素抗性弹夹(Galan等《细菌学杂志》(J.Bacteriol)1992；174：4338-4349)插入侧翼DNA片段之间以便取代tatA、tatD和tatE基因。然后将这些DNA片段转入自杀载体pCVD442(Blomfield等《分子微生物学》(Mol.Micro.)1991；5：1447-1457)。接着通过将pCVD442构建体连入野生型大肠杆菌K1后的等位转移使大肠杆菌K1tatA、tatD和tatE基因的染色体拷贝发生突变。
对受到破坏的tatA、tatD和tatE基因测试对毒力的减毒作用(Achtman等，文献同上)。
没有一种基因在分离过程中缺失时被减毒。这些基因仍然可在毒性中起作用，且为了测试这种情况，通过在tatA和tatE基因中发生缺失来制备突变体。对双重突变体测试经与野生型菌株的混合型感染的毒力的减毒作用且证明该双重突变体以0.0017的竞争指数被减毒。因此看来可以混合使用tatA、tatD和tatE基因以便产生无毒性微生物。
由于大肠杆菌K1 tatABCD基因与存在于鼠伤寒沙门氏菌基因组和脑膜炎奈瑟氏球菌基因组中的推定tatABCD基因具有相似性，所以看起来可能的是在这些和其它生物体中tat系统也可能需要毒性。通过用下列引物扩增位于tatC基因侧翼的DNA来构建鼠伤寒沙门氏菌tatC基因(SEQ ID NO.17)中的缺失：
5’-TGC TCT AGA AGG CGT TGT CGA TCC TG-3’(SEQ ID NO.65)
5’-GAA CTG CAG GAA AAG GCC GAG CAG ACT G-3’(SEQ ID NO.66)
5’-GAA CTG CAG TAC AGC CAT GTT TAC GGT-3’(SEQ ID NO.67)
5’-CAT GCA TGC GGT GTA CGA CAG TTT GCG-3’(SEQ ID NO.68)
将引物SEQ ID NO.65和SEQ ID NO.66用于扩增鼠伤寒沙门氏菌tatC基因下游的DNA序列；将引物SEQ ID NO.6 7和SEQ ID NO.68用于扩增鼠伤寒沙门氏菌tatC基因上游的DNA序列。
将所编码的tatC基因的两个区的氨基酸序列表示为SEQ ID NO.18和SEQ ID NO.19。
在将这些侧翼DNA片段克隆入pUC19中后，将非极性卡那霉素抗性弹夹(aphT)插入侧翼DNA片段之间以便取代鼠伤寒沙门氏菌tatC基因。然后将该DNA片段转入自杀载体pCVD442。接着通过将pCVD442构建体连入野生型鼠伤寒沙门氏菌菌株TML和SL1344后的等位转移使鼠伤寒沙门氏菌tatC基因的染色体拷贝发生突变。
在使用混合型和单一型感染的全身感染的小鼠模型体内对破坏的鼠伤寒沙门氏菌tatC基因测试毒力的减毒作用。对于混合型感染来说，给6-7周龄balbC小鼠经腹膜内接种104个细菌细胞。在比较3天后存在于脾中的突变体和野生型细菌的数量后来计算竞争指数。对于单一型感染来说，经腹膜内或口服给小鼠接种不同剂量并对小鼠的存活率监测17天。菌株的毒性得到弱化，SL1344 tatC和TML tatC缺失菌株的竞争指数分别为0.078和0.098。
在单一型感染中，小鼠的存活比野生型对照组小鼠的存活延长。
还通过来自脑膜炎奈瑟氏球菌的tat序列证明了序列同源性。将来自脑膜炎奈瑟氏球菌的基因序列表示为SEQ ID NO.20并将所编码的tatC的氨基酸序列表示为SEQ ID NO.21。
为了测试毒性，使用下列引物生成缺失突变体：
5’-TGCTCTAGACACATCATGGGCACACC-3’(SEQ ID NO.69)
5’-GAACTGCAGAACCGTCCACATCAGGCG-3’(SEQ ID NO.70)
5’-GAACTGCAGACCCTGCTTGCCATTCCG-3’(SEQ ID NO.71)
5’-GAACTGCAGACCCTGCTTGCCATTCCG-3’(SEQ ID NO.72)
在将DNA片段和aphT卡那霉素抗性弹夹克隆入pUC19后进行上述对鼠伤寒沙门氏菌所概括的步骤。通过将以pUC19为基础的构建体转化入野生型脑膜炎奈瑟氏球菌细胞而使脑膜炎奈瑟氏球菌tatC基因的染色体拷贝发生突变。
对所得转化体的DNA分析表明所有转化体均是部分二倍体且含有野生型和突变型tatC基因拷贝。这表明对tatC基因已经缺失的突变体的分离存在一定的选择作用。
对极性和非极性构建体的进一步研究证实转化体不在选择性培养基上生长。这提示脑膜炎奈瑟氏球菌tatC基因是该生物体体外生长所必需的。
实施例7
在本文鉴定为SEQ ID NO.22的核苷酸序列内的3981位核苷酸上根据插入的转座子来鉴定另外一种突变体。本文定义为eck1的序列显示出与来自许多细菌的几种1组糖基转移酶的序列同源性。还证实了与大肠杆菌K12的gnd基因(SEQ ID NO.22的4197-4604位核苷酸上)的序列同源性。
将翻译的大肠杆菌eck1基因表示为SEQ ID NO.26。已经如上所述测试了该基因的毒力减毒作用且证实该基因以0.025的竞争指数被减毒。
还根据DNA序列(SEQ ID NO.22)鉴定了几种可读框(ORF)。将它们中的第一种在本文中定义为MS1并将翻译产物表示为SEQ IDNO.25。证实其氨基酸序列与来自大肠杆菌血清型0111(TrEMBL数据库登记号AAD46732)的推定糖基转移酶具有50.3％的同一性。该氨基酸序列还显示出与来自大肠杆菌K1的eck1蛋白质的同源性以及与来自小肠结膜炎耶尔森氏菌(TrEMBL数据库登记号Q56917)的TrsE蛋白质的同源性。
本文中鉴定为MS2的第二种可读框具有表示为SEQ ID NO.24的基因序列。这证明了与来自小肠结膜炎耶尔森氏菌(TrEMBL数据库登记号Q56915)的推定糖基转移酶TrsC的序列同源性以及与来自大肠杆菌血清型0113(TrEMBL数据库登记号AAD50485)的糖基转移酶WbnA的序列同源性。
第三种可读框编码本文鉴定为MS3(SEQ ID NO.23)的产物。其氨基酸序列表现出与来自变异链球菌的鼠李糖基转移酶30.2％的同一性。
如果多个毒力基因成群定位于微生物的基因组上，那么表示为SEQID NO.22的基因序列可以是致病性岛的至少一部分。
实施例8
鉴定另外一种在iroCDE操纵子内带有插入的转座子的突变体。将位于小Tn5插入物侧翼的核苷酸序列表示为SEQ ID NO.27和SEQ IDNO.30。
将小Tn5转座子插在SEQ ID NO.27的1272位核苷酸上和SEQ IDNO.30的1位核苷酸上并打断iroD基因。将iroD的N-末端区表示为SEQ ID NO.29并将C-末端区表示为SEQ ID NO.31。
除iroD外，表示为SEQ ID NO.27的基因还编码带有表示为SEQ IDNO.28的氨基酸序列的部分肽。这种氨基酸序列表现出与来自伤寒沙门氏菌的推定ATP结合弹夹转运蛋白iroC 70.9％的同一性。
表示为SEQ ID NO.30的基因序列包括编码带有表示为SEQ IDNO.32的氨基酸序列的肽的可读框且它与来自伤寒沙门氏菌的iroE蛋白质具有序列同源性。
如上所述在毒力的减毒作用的模型中对基因测试表明以0.107的竞争指数对iroD基因进行了减毒。已经通过P1转导将iroD基因中的小Tn5突变重新引入野生型大肠杆菌K1菌株。还以0.1的竞争指数对所得转导体的毒力进行了减毒。这表明减毒表型与iroD内的插入物连接。然而，因对大肠杆菌K1 iroE基因的极性作用而导致减毒是可能的。
实施例9
用表示为SEQ ID NO.33的核苷酸序列内插入的转座子鉴定另外一种突变体。将该转座子插在SEQ ID NO.33的2264位核苷酸上。该核苷酸序列表现出与大肠杆菌K12(EMBL登记号AE000456)的aslA/hemY区的序列同源性。aslA编码芳基硫酸酯酶同源物，而hemY与血红素IX的生物合成有关。这证明受到破坏的区至少部分与大肠杆菌K12的aslA/hemY区相同。
将转座子插在SEQ ID NO.33的2264位核苷酸上。该插入位点是来自hemY基因的终止密码子下游的216个核苷酸和来自aslA基因的起始密码子上游的472个核苷酸。
如上所述对新区测试毒力的减毒作用且证实该新区以0.033的竞争指数被减毒。已经通过P1转导将该区中的小Tn5突变重新引入野生型大肠杆菌K1菌株。还以0.1的竞争指数对所得的转导体的毒力进行了减毒。这表明减毒表型与该区中的转座子插入物连接。不过，如上所述构建aslA的极性和非极性缺失突变体并测试其对毒力的减毒作用。
无论是极性还是非极性突变体的毒力均没有弱化且这证明原始转座子突变体的减毒作用并不是因对aslA基因的极性作用而导致的。这表明转座子正在破坏在aslA与hemY之间的基因间隔区内编码的某些其它功能。例如，可能存在某些在该区内编码未翻译的RNA分子诸如与oxyS相似的调节RNA(Altuvia等《细胞》(Cell)1997；90：43-53)。另一方面，转座子可能破坏例如与DNA复制相关的某些DNA结构。该DNA区还存在于病原体鼠伤寒沙门氏菌中，从而提示它对其它生物体中的致病性来说可能是重要的。可以将该区(SEQ ID NO.33)用作靶物以鉴定抗微生物药。
实施例10
鉴定另外一种突变体并克隆位于小Tn5插入物侧翼的DNA区且它具有表示为SEQ ID NO.34的核苷酸序列。由于mtd2基因产物起胞嘧啶特异性甲基转移酶的作用，所以该核苷酸序列与橙色滑柱菌(EMBL登记号P25265)的mtd2基因具有同源性。在大肠杆菌K12中没有发现mtd2基因且它可以代表致病性岛。
小Tn5转座子插入物定位于SEQ ID NO.34的4773和3764位核苷酸上且证实它可打断mtd2基因。
将mtd2基因的氨基酸序列表示为SEQ ID NO.43。
如上所述对大肠杆菌K1 mtd2基因测试对毒力的减毒作用且证实该基因以0.073的竞争指数被减毒。
除mtd2基因外，还使用本文分别鉴定为MS4-MS16、SEQ IDNOS.48-44和42-35的翻译产物鉴定了一系列可读框。如果可读框定位于潜在致病性岛中，那么这些基因中的突变也可以使毒力弱化。此外，由于已知大肠杆菌和其它细菌可以编码核苷酸序列中不同形式的肽类，所以这些蛋白质中的某些的编码区可以重叠。此外，显示出以Val开始的任意氨基酸序列实际上可以以Met开始。
序列表
<110>Microscience Limited
<120>毒力基因和蛋白质及其用途
<130>REP05921WO
<140>
<141>
<160>72
<170>PatentIn 2.1版
<210>1
<211>4333
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(1017)..(2549)
<400>1
ccattactca gaatgggcgg atacacaata aaaattgttc ttcttattac cgcataaccg 60
atgccgaggc acaaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta 120
tcagaatttt ctaaattatt tctgatacgt ttgaatatcc agacgcacag cgtcgtcatg 180
accactaaca ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat 240
ttgtcataca atgacagtcc aggccaactt tccgctttcc ctttgacgta ttgcagcata 300
ataaattgcg gcaatgtcag tagggggatg gctgttaaca tcgggatacc tacacgttcg 360
acacgtactt tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtac 420
ccggatataa cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtc 480
agccataatg acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa 540
atatgaaaag ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca 600
cgttgcgcgg gtactgggtt catatatggt taactaatct cggatttttc gtcttatccc 660
tgtcgggtta tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc 720
aggcttcaag gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg 780
taagacggtt gataaataaa tttgctggca aaccctacac gaagtcgatg cttctgtctt 840
taggagaagc acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagtgt 900
gctgaacgat accgggattc tgttgtcgga atggcaggtt atccattaaa atagatcgga 960
tcgatataag cacacaaagg gggaagtgct tactaattat gaaacataaa ctacaa atg 1019
Met
1
atg aaa atg cgt tgg ttg agt gct gca gta atg tta acc ctg tat aca 1067
Met Lys Met Arg Trp Leu Ser Ala Ala Val Met Leu Thr Leu Tyr Thr
5 10 15
tct tca agc tgg gct ttc agt att gat gat gtc gca aag caa gct caa 1115
Ser Ser Ser Trp Ala Phe Ser Ile Asp Asp Val Ala Lys Gln Ala Gln
20 25 30
tcc tta gcc ggg aaa ggc tat gag gcg ccc aaa agc aac ttg ccc tcc 1163
Ser Leu Ala Gly Lys Gly Tyr Glu Ala Pro Lys Ser Asn Leu Pro Ser
35 40 45
gtt ttc cgc gat atg aaa tac gcg gac tat cag cag atc cag ttt aat 1211
Val Phe Arg Asp Met Lys Tyr Ala Asp Tyr Gln Gln Ile Gln Phe Asn
50 55 60 65
cat gac aaa gcg tac tgg aac aat ctg aag acc cca ttc aaa ctc gag 1259
His Asp Lys Ala Tyr Trp Asn Asn Leu Lys Thr Pro Phe Lys Leu Glu
70 75 80
ttc tac cat cag ggt atg tac ttc gat acc ccg gtc aaa ata aat gaa 1307
Phe Tyr His Gln Gly Met Tyr Phe Asp Thr Pro Val Lys Ile Asn Glu
85 90 95
gtg act gcc acc gca gtc aaa cga atc aaa tac agc ccg gat tat ttc 1355
Val Thr Ala Thr Ala Val Lys Arg Ile Lys Tyr Ser Pro Asp Tyr Phe
100 105 110
act ttc ggc gat gtt cag cat gac aaa gac acg gta aaa gac ctt ggt 1403
Thr Phe Gly Asp Val Gln His Asp Lys Asp Thr Val Lys Asp Leu Gly
115 120 125
ttt gcc ggt ttc aaa gtg ctt tac ccg atc aac agc aaa gat aaa aac 1451
Phe Ala Gly Phe Lys Val Leu Tyr Pro Ile Asn Ser Lys Asp Lys Asn
130 135 140 145
gat gaa atc gtc agc atg ctc ggg gcc agc tat ttc cgc gtg att ggt 1499
Asp Glu Ile Val Ser Met Leu Gly Ala Ser Tyr Phe Arg Val Ile Gly
150 155 160
gca ggt cag gtt tat ggc ctt tct gca cgc ggc ctg gca att gat acc 1547
Ala Gly Gln Val Tyr Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp Thr
165 170 175
gcc ttg cca tcg ggt gaa gaa ttt cca cgc ttc aaa gag ttc tgg atc 1595
Ala Leu Pro Ser Gly Glu Glu Phe Pro Arg Phe Lys Glu Phe Trp Ile
180 185 190
gag cgt cca aaa ccg act gat aaa cgt tta acc att tat gca ttg ctt 1643
Glu Arg Pro Lys Pro Thr Asp Lys Arg Leu Thr Ile Tyr Ala Leu Leu
195 200 205
gac tcg ccg cgc gcg aca ggt gct tac aaa ttc gta gtt atg cca gga 1691
Asp Ser Pro Arg Ala Thr Gly Ala Tyr Lys Phe Val Val Met Pro Gly
210 215 220 225
cgt gac acg gtt gtg gat gtg cag tcg aaa atc tat ctg cgc gat aaa 1739
Arg Asp Thr Val Val Asp Val Gln Ser Lys Ile Tyr Leu Arg Asp Lys
230 235 240
gtc ggc aaa ctg ggg gtt gca ccg tta acc agt atg ttc ctg ttt ggg 1787
Val Gly Lys Leu Gly Val Ala Pro Leu Thr Ser Met Phe Leu Phe Gly
245 250 255
ccg aac caa ccg tcg cct gca aat aac tat cgt ccg gag ttg cac gac 1835
Pro Asn Gln Pro Ser Pro Ala Asn Asn Tyr Arg Pro Glu Leu His Asp
260 265 270
tct aac ggt ctg tct atc cat gct ggt aat ggc gaa tgg atc tgg cgt 1883
Ser Asn Gly Leu Ser Ile His Ala Gly Asn Gly Glu Trp Ile Trp Arg
275 280 285
ccg ttg aat aac ccg aaa cat tta gcg gtc agc agc ttc tcg atg gaa 1931
Pro Leu Asn Asn Pro Lys His Leu Ala Val Ser Ser Phe Ser Met Glu
290 295 300 305
aac ccg caa ggc ttc ggt cta ttg cag cgt ggt cgt gat ttc tcc cgc 1979
Asn Pro Gln Gly Phe Gly Leu Leu Gln Arg Gly Arg Asp Phe Ser Arg
310 315 320
ttt gaa gat ctc gat gat cgt tac gat ctt cgt cca agc gca tgg gtg 2027
Phe Glu Asp Leu Asp Asp Arg Tyr Asp Leu Arg Pro Ser Ala Trp Val
325 330 335
act ccg aaa ggg gag tgg ggc aaa ggc agc gtt gag ctg gtg gaa att 2075
Thr Pro Lys Gly Glu Trp Gly Lys Gly Ser Val Glu Leu Val Glu Ile
340 345 350
cca acc aac gat gaa acc aac gat aac atc gtc gct tac tgg acg ccg 2123
Pro Thr Asn Asp Glu Thr Asn Asp Asn Ile Val Ala Tyr Trp Thr Pro
355 360 365
gat cag ctg ccg gag ccg ggt aaa gag atg aac ttt aaa tac acc atc 2171
Asp Gln Leu Pro Glu Pro Gly Lys Glu Met Asn Phe Lys Tyr Thr Ile
370 375 380 385
acc ttc agc cgt gat gaa gac aaa ctg cat gcg cca gat aac gca tgg 2219
Thr Phe Ser Arg Asp Glu Asp Lys Leu His Ala Pro Asp Asn Ala Trp
390 395 400
gtg caa caa acg cgt cgt tca acg ggg gat gtg aag cag tcg aac ctg 2267
Val Gln Gln Thr Arg Arg Ser Thr Gly Asp Val Lys Gln Ser Asn Leu
405 410 415
att cgc cag cct gac ggt act atc gcc ttt gtg gtc gat ttt acc ggc 2315
Ile Arg Gln Pro Asp Gly Thr Ile Ala Phe Val Val Asp Phe Thr Gly
420 425 430
gct gag atg aaa aaa ctg cca gag gat acc ccg gtc aca gcg caa acc 2363
Ala Glu Met Lys Lys Leu Pro Glu Asp Thr Pro Val Thr Ala Gln Thr
435 440 445
agc att ggt gat aat ggt gag ata gtt gaa agc acg gtg cgt tat aac 2411
Ser Ile Gly Asp Asn Gly Glu Ile Val Glu Ser Thr Val Arg Tyr Asn
450 455 460 465
ccg gtt acc aaa ggc tgg cgt ctg gtg atg cgt gtg aaa gtg aaa gat 2459
Pro Val Thr Lys Gly Trp Arg Leu Val Met Arg Val Lys Val Lys Asp
470 475 480
gcc aag aaa acc act gaa atg cgt gct gcg ctg gtg aat gcc gat cag 2507
Ala Lys Lys Thr Thr Glu Met Arg Ala Ala Leu Val Asn Ala Asp Gln
485 490 495
acg ttg agt gaa acc tgg agc tac cag tta cct gcc aat gaa 2549
Thr Leu Ser Glu Thr Trp Ser Tyr Gln Leu Pro Ala Asn Glu
500 505 510
taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag cggcattgcc 2609
gaagactgat atccgcgccg ttcatcaggc gctggatgcc gaacaccgca cctgggcgcg 2669
ggaggatgac tccccgcaag gctcggtaaa ggcgcgtctg gaacaagcct ggccagattc 2729
acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctaa aggcgatgcc 2789
agaagtaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg taggccgttt 2849
ctgggatcgc ctgcgtggac gcgatgtgac gccgcgctat ctggctcgtt tgaccaaaga 2909
agagcaggag agtgagcaaa agtggcgtac cgtcggtacc atccgccgtt acattctgtt 2969
gatcctgacg ctcgcgcaaa ctgttgtcgc gacctggtat atgaagacca ttcttcctta 3029
tcaggggtgg gcgctgatta atcctatgga tatggttggt caggatgtgt gggtttcctt 3089
tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctctttg cggtactgtt 3149
ctgttgggtg tccgccggat tctggaccgg cgttgatggg cttcctgcaa ctgcttattg 3209
gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc 3269
atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct 3329
tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacatttt gatgtctaca 3389
ttcttagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc 3449
ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgc cgtcgcgtga 3509
agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca 3569
tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttgtgcggc ctggtgcgcc 3629
tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggcatgg 3689
atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag 3749
ccggtttgca cttctggcaa cttggcgagt cgcactactg ggggcataac gcgattatcc 3809
gcgtgaaacc gtttatcgag cactgtgcac tggctccgct gccgggcgaa ggttcttttg 3869
ccggttcaat cctgtcacat gacttcgtgg aagcggcgtt gatgcgccgt gcaggttggg 3929
gggtctggat tgcttacgat ctcccgggtt cttatgaaga attaccgcct aacttgcttg 3989
atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc 4049
tggtgaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc 4109
tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtacatg 4169
cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggta tggccgcagt 4229
ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga 4289
agctattgag cattttgctt atctggtgca aaggaacgaa agaa 4333
<210>2
<211>511
<212>PRT
<213>大肠杆菌
<400>2
Met Met Lys Met Arg Trp Leu Ser Ala Ala Val Met Leu Thr Leu Tyr
1 5 10 15
Thr Ser Ser Ser Trp Ala Phe Ser Ile Asp Asp Val Ala Lys Gln Ala
20 25 30
Gln Ser Leu Ala Gly Lys Gly Tyr Glu Ala Pro Lys Ser Asn Leu Pro
35 40 45
Ser Val Phe Arg Asp Met Lys Tyr Ala Asp Tyr Gln Gln Ile Gln Phe
50 55 60
Asn His Asp Lys Ala Tyr Trp Asn Asn Leu Lys Thr Pro Phe Lys Leu
65 70 75 80
Glu Phe Tyr His Gln Gly Met Tyr Phe Asp Thr Pro Val Lys Ile Asn
85 90 95
Glu Val Thr Ala Thr Ala Val Lys Arg Ile Lys Tyr Ser Pro Asp Tyr
100 105 110
Phe Thr Phe Gly Asp Val Gln His Asp Lys Asp Thr Val Lys Asp Leu
115 120 125
Gly Phe Ala Gly Phe Lys Val Leu Tyr Pro Ile Asn Ser Lys Asp Lys
130 135 140
Asn Asp Glu Ile Val Ser Met Leu Gly Ala Ser Tyr Phe Arg Val Ile
145 150 155 160
Gly Ala Gly Gln Val Tyr Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp
165 170 175
Thr Ala Leu Pro Ser Gly Glu Glu Phe Pro Arg Phe Lys Glu Phe Trp
180 185 190
Ile Glu Arg Pro Lys Pro Thr Asp Lys Arg Leu Thr Ile Tyr Ala Leu
195 200 205
Leu Asp Ser Pro Arg Ala Thr Gly Ala Tyr Lys Phe Val Val Met Pro
210 215 220
Gly Arg Asp Thr Val Val Asp Val Gln Ser Lys Ile Tyr Leu Arg Asp
225 230 235 240
Lys Val Gly Lys Leu Gly Val Ala Pro Leu Thr Ser Met Phe Leu Phe
245 250 255
Gly Pro Asn Gln Pro Ser Pro Ala Asn Asn Tyr Arg Pro Glu Leu His
260 265 270
Asp Ser Asn Gly Leu Ser Ile His Ala Gly Asn Gly Glu Trp Ile Trp
275 280 285
Arg Pro Leu Asn Asn Pro Lys His Leu Ala Val Ser Ser Phe Ser Met
290 295 300
Glu Asn Pro Gln Gly Phe Gly Leu Leu Gln Arg Gly Arg Asp Phe Ser
305 310 315 320
Arg Phe Glu Asp Leu Asp Asp Arg Tyr Asp Leu Arg Pro Ser Ala Trp
325 330 335
Val Thr Pro Lys Gly Glu Trp Gly Lys Gly Ser Val Glu Leu Val Glu
340 345 350
Ile Pro Thr Asn Asp Glu Thr Asn Asp Asn Ile Val Ala Tyr Trp Thr
355 360 365
Pro Asp Gln Leu Pro Glu Pro Gly Lys Glu Met Asn Phe Lys Tyr Thr
370 375 380
Ile Thr Phe Ser Arg Asp Glu Asp Lys Leu His Ala Pro Asp Asn Ala
385 390 395 400
Trp Val Gln Gln Thr Arg Arg Ser Thr Gly Asp Val Lys Gln Ser Asn
405 410 415
Leu Ile Arg Gln Pro Asp Gly Thr Ile Ala Phe Val Val Asp Phe Thr
420 425 430
Gly Ala Glu Met Lys Lys Leu Pro Glu Asp Thr Pro Val Thr Ala Gln
435 440 445
Thr Ser Ile Gly Asp Asn Gly Glu Ile Val Glu Ser Thr Val Arg Tyr
450 455 460
Asn Pro Val Thr Lys Gly Trp Arg Leu Val Met Arg Val Lys Val Lys
465 470 475 480
Asp Ala Lys Lys Thr Thr Glu Met Arg Ala Ala Leu Val Asn Ala Asp
485 490 495
Gln Thr Leu Ser Glu Thr Trp Ser Tyr Gln Leu Pro Ala Asn Glu
500 505 510
<210>3
<211>574
<212>DNA
<213>大肠杆菌
<400>3
ttcgttgatc ctgtcaccgt ttgttcggtt atttccagcc gtgccaccgt tggtctgcga 60
accaaacgct ggaaactgtt ccctgatccc ggaagagtat tcaccgccgc aggtgctggt 120
tgataccgat cggttccttg agatgaatcg tcaatgctcc cttgatgatg gttttatgca 180
cgcggtgttt aacccgtcat ttaacgctct ggcaaccgca atggcgaccg cgcgtcaccg 240
cgccagcaag gtgctggaaa tcgcccgtga ccgccacgtt gaacaggcgc tgaacgagac 300
gccagagaag ctgaatcgcg atcgtcgcct ggtgctgcta agcgatccgg tgacgatggc 360
ccgtctgcat ttccgcgtct ggaattcccc ggagagatat tcttcatggg tgagttatta 420
cgaagggata aagctcaatc cactggcatt gcgtaaaccg gatgcggctt cgcaataaaa 480
acgtagttgc ctgatgcgct acgcttatca ggcctacatc gttcctgcaa tttattgatt 540
ttgcaagatt ttgtaggtcg gataaggcgt tcac 574
<210>4
<211>1478
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(25)..(1449)
<400>4
gggataatgc ctgaggggcc tgta atg cgt atc ggc atg cgg ttg ttg ctg 51
Met Arg Ile Gly Met Arg Leu Leu Leu
1 5
ggc tat ttt tta ctg gtg gcg gtg gcg gcc tgg ttc gta ctg gct att 99
Gly Tyr Phe Leu Leu Val Ala Val Ala Ala Trp Phe Val Leu Ala Ile
10 15 20 25
ttt gtc aaa gaa gtt aaa ccg ggc gtg cga aga gca acc gag ggg acg 147
Phe Val Lys Glu Val Lys Pro Gly Val Arg Arg Ala Thr Glu Gly Thr
30 35 40
tta atc gac acc gca acg ttg ctg gcg gag ctg gcg cgt ccc gat ttg 195
Leu Ile Asp Thr Ala Thr Leu Leu Ala Glu Leu Ala Arg Pro Asp Leu
45 50 55
ctc tct ggg gac cca acg cat ggg caa ctg gcg cag gcg ttt aat cag 243
Leu Ser Gly Asp Pro Thr His Gly Gln Leu Ala Gln Ala Phe Asn Gln
60 65 70
cta caa cat cgc ccg ttt cgc gcc aat atc ggt ggc att aac aaa gtg 291
Leu Gln His Arg Pro Phe Arg Ala Asn Ile Gly Gly Ile Asn Lys Val
75 80 85
cgc aac gaa tat cat gtc tat atg acc gat gcg cag ggc aaa gta ttg 339
Arg Asn Glu Tyr His Val Tyr Met Thr Asp Ala Gln Gly Lys Val Leu
90 95 100 105
ttc gat tcg gca aat aaa gcc gtt gga cag gat tat tcg cgc tgg aat 387
Phe Asp Ser Ala Asn Lys Ala Val Gly Gln Asp Tyr Ser Arg Trp Asn
110 115 120
gac gtc tgg cta acg ttg cgt ggt cag tat ggt gcg cgc agc acg ttg 435
Asp Val Trp Leu Thr Leu Arg Gly Gln Tyr Gly Ala Arg Ser Thr Leu
125 130 135
caa aat cct gcc gat ccc gaa agt tct gtg atg tat gtt gcc gcg ccg 483
Gln Asn Pro Ala Asp Pro Glu Ser Ser Val Met Tyr Val Ala Ala Pro
140 145 150
att atg gac ggc tcg cgg ctt att ggc gtt ttg agc gta ggc aaa ccg 531
Ile Met Asp Gly Ser Arg Leu Ile Gly Val Leu Ser Val Gly Lys Pro
155 160 165
aac gcg gcg atg gct ccg gtc att aag cgt agc gag cgg cga att tta 579
Asn Ala Ala Met Ala Pro Val Ile Lys Arg Ser Glu Arg Arg Ile Leu
170 175 180 185
tgg gcc agc gcc att ttg ttg ggg att gca ctg gtg att ggc gca ggc 627
Trp Ala Ser Ala Ile Leu Leu Gly Ile Ala Leu Val Ile Gly Ala Gly
190 195 200
atg gtt tgg tgg atc aac cgc tct att gcc agg ctc act cgc tat gct 675
Met Val Trp Trp Ile Asn Arg Ser Ile Ala Arg Leu Thr Arg Tyr Ala
205 210 215
gat tcc gtc act gac aat aag ccc gtt cct ctc ccc gat ctc ggt agt 723
Asp Ser Val Thr Asp Asn Lys Pro Val Pro Leu Pro Asp Leu Gly Ser
220 225 230
agc gag ttg cgt aaa ctc gcg cag gcg ctg gaa agt atg cgc gtg aag 771
Ser Glu Leu Arg Lys Leu Ala Gln Ala Leu Glu Ser Met Arg Val Lys
235 240 245
ctg gaa ggg aaa aac tat att gag cag tat gtt tat gcg tta act cat 819
Leu Glu Gly Lys Asn Tyr Ile Glu Gln Tyr Val Tyr Ala Leu Thr His
250 255 260 265
gag cta aaa agc cca ctg gcg gcg att cgt ggc gcg gcg gaa att tta 867
Glu Leu Lys Ser Pro Leu Ala Ala Ile Arg Gly Ala Ala Glu Ile Leu
270 275 280
cgc gaa ggt ccg ccg ccg gaa gtg gtg gct cgt ttt acc gac aac att 915
Arg Glu Gly Pro Pro Pro Glu Val Val Ala Arg Phe Thr Asp Asn Ile
285 290 295
ctg acg caa aat gcg cga atg cag gca ctg gtg gaa acg tta cta cgc 963
Leu Thr Gln Asn Ala Arg Met Gln Ala Leu Val Glu Thr Leu Leu Arg
300 305 310
cag gca aga ctg gag aat cgt cag gaa gtc gtt ctg act gct gtt gat 1011
Gln Ala Arg Leu Glu Asn Arg Gln Glu Val Val Leu Thr Ala Val Asp
315 320 325
gtg gcg gca tta ttt cgc cgc gtc agc gaa gcg cgc acc gtg cag ttg 1059
Val Ala Ala Leu Phe Arg Arg Val Ser Glu Ala Arg Thr Val Gln Leu
330 335 340 345
gca gaa aaa aac atc act ttg cat gtt atg cct act gag gtt aac gtt 1107
Ala Glu Lys Asn Ile Thr Leu His Val Met Pro Thr Glu Val Asn Val
350 355 360
gct tct gaa ccg gcg tta ctg gag cag gcg ctg ggg aat tta ctg gat 1155
Ala Ser Glu Pro Ala Leu Leu Glu Gln Ala Leu Gly Asn Leu Leu Asp
365 370 375
aac gcc atc gat ttt act ccc gag agc ggt tgc ata acg cta agc gcc 1203
Asn Ala Ile Asp Phe Thr Pro Glu Ser Gly Cys Ile Thr Leu Ser Ala
380 385 390
gaa gtg gat cag gaa tac gtc acc ctt aag gtg ctg gat acc ggt agt 1251
Glu Val Asp Gln Glu Tyr Val Thr Leu Lys Val Leu Asp Thr Gly Ser
395 400 405
ggg att cct gac tac gcg ctg tca cgt att ttt gaa cgc ttt tac tct 1299
Gly Ile Pro Asp Tyr Ala Leu Ser Arg Ile Phe Glu Arg Phe Tyr Ser
410 415 420 425
ttg ccg cgt gca aat ggg caa aaa agc agc ggt ctg ggg ttg gcg ttt 1347
Leu Pro Arg Ala Asn Gly Gln Lys Ser Ser Gly Leu Gly Leu Ala Phe
430 435 440
gtc agt gag gtc gcc cgt ttg ttt aac ggc gaa gtc acg ctg cgc aac 1395
Val Ser Glu Val Ala Arg Leu Phe Asn Gly Glu Val Thr Leu Arg Asn
445 450 455
gtg cag gaa ggt ggc gtg ctg gcc tcg ctt cga ctt cac cgt cac ttc 1443
Val Gln Glu Gly Gly Val Leu Ala Ser Leu Arg Leu His Arg His Phe
460 465 470
aca tag cttcaaattc ttcccacata gtcttcgta 1478
Thr
475
<210>5
<211>474
<212>PRT
<213>大肠杆菌
<400>5
Met Arg Ile Gly Met Arg Leu Leu Leu Gly Tyr Phe Leu Leu Val Ala
1 5 10 15
Val Ala Ala Trp Phe Val Leu Ala Ile Phe Val Lys Glu Val Lys Pro
20 25 30
Gly Val Arg Arg Ala Thr Glu Gly Thr Leu Ile Asp Thr Ala Thr Leu
35 40 45
Leu Ala Glu Leu Ala Arg Pro Asp Leu Leu Ser Gly Asp Pro Thr His
50 55 60
Gly Gln Leu Ala Gln Ala Phe Asn Gln Leu Gln His Arg Pro Phe Arg
65 70 75 80
Ala Asn Ile Gly Gly Ile Asn Lys Val Arg Asn Glu Tyr His Val Tyr
85 90 95
Met Thr Asp Ala Gln Gly Lys Val Leu Phe Asp Ser Ala Asn Lys Ala
100 105 110
Val Gly Gln Asp Tyr Ser Arg Trp Asn Asp Val Trp Leu Thr Leu Arg
115 120 125
Gly Gln Tyr Gly Ala Arg Ser Thr Leu Gln Asn Pro Ala Asp Pro Glu
130 135 140
Ser Ser Val Met Tyr Val Ala Ala Pro Ile Met Asp Gly Ser Arg Leu
145 150 155 160
Ile Gly Val Leu Ser Val Gly Lys Pro Asn Ala Ala Met Ala Pro Val
165 170 175
Ile Lys Arg Ser Glu Arg Arg Ile Leu Trp Ala Ser Ala Ile Leu Leu
180 185 190
Gly Ile Ala Leu Val Ile Gly Ala Gly Met Val Trp Trp Ile Asn Arg
195 200 205
Ser Ile Ala Arg Leu Thr Arg Tyr Ala Asp Ser Val Thr Asp Asn Lys
210 215 220
Pro Val Pro Leu Pro Asp Leu Gly Ser Ser Glu Leu Arg Lys Leu Ala
225 230 235 240
Gln Ala Leu Glu Ser Met Arg Val Lys Leu Glu Gly Lys Asn Tyr Ile
245 250 255
Glu Gln Tyr Val Tyr Ala Leu Thr His Glu Leu Lys Ser Pro Leu Ala
260 265 270
Ala Ile Arg Gly Ala Ala Glu Ile Leu Arg Glu Gly Pro Pro Pro Glu
275 280 285
Val Val Ala Arg Phe Thr Asp Asn Ile Leu Thr Gln Asn Ala Arg Met
290 295 300
Gln Ala Leu Val Glu Thr Leu Leu Arg Gln Ala Arg Leu Glu Asn Arg
305 310 315 320
Gln Glu Val Val Leu Thr Ala Val Asp Val Ala Ala Leu Phe Arg Arg
325 330 335
Val Ser Glu Ala Arg Thr Val Gln Leu Ala Glu Lys Asn Ile Thr Leu
340 345 350
His Val Met Pro Thr Glu Val Asn Val Ala Ser Glu Pro Ala Leu Leu
355 360 365
Glu Gln Ala Leu Gly Asn Leu Leu Asp Asn Ala Ile Asp Phe Thr Pro
370 375 380
Glu Ser Gly Cys Ile Thr Leu Ser Ala Glu Val Asp Gln Glu Tyr Val
385 390 395 400
Thr Leu Lys Val Leu Asp Thr Gly Ser Gly Ile Pro Asp Tyr Ala Leu
405 410 415
Ser Arg Ile Phe Glu Arg Phe Tyr Ser Leu Pro Arg Ala Asn Gly Gln
420 425 430
Lys Ser Ser Gly Leu Gly Leu Ala Phe Val Ser Glu Val Ala Arg Leu
435 440 445
Phe Asn Gly Glu Val Thr Leu Arg Asn Val Gln Glu Gly Gly Val Leu
450 455 460
Ala Ser Leu Arg Leu His Arg His Phe Thr
465 470
<210>6
<211>128
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(1)..(126)
<400>6
atg aaa ggt cgc ctg tta gat gct gtc ccg ctc agt tcc cta acg ggc 48
Met Lys Gly Arg Leu Leu Asp Ala Val Pro Leu Ser Ser Leu Thr Gly
1 5 10 15
gtt ggc gca gcg ctt agt aac aag ctg gcg aaa atc aac ctg cat acc 96
Val Gly Ala Ala Leu Ser Asn Lys Leu Ala Lys Ile Asn Leu His Thr
20 25 30
gta cag gat tta ctc tta cac ctt cct ctg cg 128
Val Gln Asp Leu Leu Leu His Leu Pro Leu
35 40
<210>7
<211>42
<212>PRT
<213>大肠杆菌
<400>7
Met Lys Gly Arg Leu Leu Asp Ala Val Pro Leu Ser Ser Leu Thr Gly
1 5 10 15
Val Gly Ala Ala Leu Ser Asn Lys Leu Ala Lys Ile Asn Leu His Thr
20 25 30
Val Gln Asp Leu Leu Leu His Leu Pro Leu
35 40
<210>8
<211>1174
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(121)..(837)
<400>8
agatgcacga tcgagtaggc cggataaggc gtttacgccg catccagcat ggaaaacgcg 60
cactttgtta tcaatctggg gccagcaaat gctggcctga tttgttcttg agggaagact 120
atg atg cgc aaa atg ctg ctg gcg gca gca ctt tca gtg acg gca atg 168
Met Met Arg Lys Met Leu Leu Ala Ala Ala Leu Ser Val Thr Ala Met
1 5 10 15
acc gct cac gcc gac tac cag tgc agc gtc acg ccg cgt gac gat gtg 216
Thr Ala His Ala Asp Tyr Gln Cys Ser Val Thr Pro Arg Asp Asp Val
20 25 30
att gtc agc ccg caa acc gtg cag gtg aag ggc gaa aac ggc aat ctg 264
Ile Val Ser Pro Gln Thr Val Gln Val Lys Gly Glu Asn Gly Asn Leu
35 40 45
gtg atc acg cca gac ggc aac gtg atg tat aac ggt aag caa tat tcc 312
Val Ile Thr Pro Asp Gly Asn Val Met Tyr Asn Gly Lys Gln Tyr Ser
50 55 60
ctg aat gcc gcc cag cgc gag cag gcg aag gat tat cag gct gaa cta 360
Leu Asn Ala Ala Gln Arg Glu Gln Ala Lys Asp Tyr Gln Ala Glu Leu
65 70 75 80
cgt agc acc ctg ccg tgg att gat gga ggc gcg aaa agc cgc gtc gag 408
Arg Ser Thr Leu Pro Trp Ile Asp Gly Gly Ala Lys Ser Arg Val Glu
85 90 95
aaa gct cgt att gcg ctg gat aaa att atc gtt cag gag atg ggc gaa 456
Lys Ala Arg Ile Ala Leu Asp Lys Ile Ile Val Gln Glu Met Gly Glu
100 105 110
agc agc aaa atg cgc agc cgt ctg acc aaa ctt gat gcg cag ctg aaa 504
Ser Ser Lys Met Arg Ser Arg Leu Thr Lys Leu Asp Ala Gln Leu Lys
115 120 125
gag cag atg aac cgc att atc gaa acg cgc agc gat ggc ctg acg ttt 552
Glu Gln Met Asn Arg Ile Ile Glu Thr Arg Ser Asp Gly Leu Thr Phe
130 135 140
cac tat aaa gcc att gat cag gtt cgt gcc gaa ggc cag caa tta gtg 600
His Tyr Lys Ala Ile Asp Gln Val Arg Ala Glu Gly Gln Gln Leu Val
145 150 155 160
aat cag gca atg ggc gga att tta cag gac agc att aat gaa atg ggc 648
Asn Gln Ala Met Gly Gly Ile Leu Gln Asp Ser Ile Asn Glu Met Gly
165 170 175
gcg aaa gcg gtg ctg aaa agc ggc ggt aac cca tta cag aac gtg ctg 696
Ala Lys Ala Val Leu Lys Ser Gly Gly Asn Pro Leu Gln Asn Val Leu
180 185 190
gga agc ctg ggc ggc ctg caa tcc tca atc caa acc gag tgg aaa aag 744
Gly Ser Leu Gly Gly Leu Gln Ser Ser Ile Gln Thr Glu Trp Lys Lys
195 200 205
cag gaa aaa gat ttc cag cag ttt ggc aaa gat gtt tgt agc cgc gtt 792
Gln Glu Lys Asp Phe Gln Gln Phe Gly Lys Asp Val Cys Ser Arg Val
210 215 220
gtg act ctg gaa gat agc cgc aaa gcc ctg gtc ggg aat tta aaa 837
Val Thr Leu Glu Asp Ser Arg Lys Ala Leu Val Gly Asn Leu Lys
225 230 235
taatcctcta ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt 897
acctgcctct aactttgtaa gggcgaattc tgcagatatc catcacactg gcggccgctc 957
gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc 1017
gtcgttttac aaccgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 1077
agcacatccc cctttcgcca gctggcgtaa tagcgaaaag gcccgcaccg atcgcccttc 1137
caacagttgc gcacctgatg gccaatggac gcgcctg 1174
<210>9
<211>239
<212>PRT
<213>大肠杆菌
<400>9
Met Met Arg Lys Met Leu Leu Ala Ala Ala Leu Ser Val Thr Ala Met
1 5 10 15
Thr Ala His Ala Asp Tyr Gln Cys Ser Val Thr Pro Arg Asp Asp Val
20 25 30
Ile Val Ser Pro Gln Thr Val Gln Val Lys Gly Glu Asn Gly Asn Leu
35 40 45
Val Ile Thr Pro Asp Gly Asn Val Met Tyr Asn Gly Lys Gln Tyr Ser
50 55 60
Leu Asn Ala Ala Gln Arg Glu Gln Ala Lys Asp Tyr Gln Ala Glu Leu
65 70 75 80
Arg Ser Thr Leu Pro Trp Ile Asp Gly Gly Ala Lys Ser Arg Val Glu
85 90 95
Lys Ala Arg Ile Ala Leu Asp Lys Ile Ile Val Gln Glu Met Gly Glu
100 105 110
Ser Ser Lys Met Arg Ser Arg Leu Thr Lys Leu Asp Ala Gln Leu Lys
115 120 125
Glu Gln Met Asn Arg Ile Ile Glu Thr Arg Ser Asp Gly Leu Thr Phe
130 135 140
His Tyr Lys Ala Ile Asp Gln Val Arg Ala Glu Gly Gln Gln Leu Val
145 150 155 160
Asn Gln Ala Met Gly Gly Ile Leu Gln Asp Ser Ile Asn Glu Met Gly
165 170 175
Ala Lys Ala Val Leu Lys Ser Gly Gly Asn Pro Leu Gln Asn Val Leu
180 185 190
Gly Ser Leu Gly Gly Leu Gln Ser Ser Ile Gln Thr Glu Trp Lys Lys
195 200 205
Gln Glu Lys Asp Phe Gln Gln Phe Gly Lys Asp Val Cys Ser Arg Val
210 215 220
Val Thr Leu Glu Asp Ser Arg Lys Ala Leu Val Gly Asn Leu Lys
225 230 235
<210>10
<211>3406
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(1007)..(1276)
<220>
<221>CDS
<222>(1280)..(1792)
<220>
<221>CDS
<222>(1798)..(2574)
<220>
<221>CDS
<222>(2604)..(3398)
<400>10
gatgatggtg atggagcgta tttacggcat tccggtgtct gatgttgcga cgctggagaa 60
aaacggcacc aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt 120
ctttcgcgac agctttttcc atgctgatat gcaccctggc aacatcttcg taagctatga 180
acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa 240
agaagataaa cgctatctgg cggaaaactt tatcgccttc tttaatcgcg actatcgcaa 300
agtggcagag ctacacgtcg attctggttg ggtgccacca gataccaacg ttgaagagtt 360
cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc 420
gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca 480
gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct 540
ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc ctggagtcgt ggattaaaga 600
tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga 660
aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca 720
gcatagtgtt ggtaagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatt 780
cgcgttattt tctcggaatt ggcgctacgt tagtatttaa gtggcacatt cttgttggtc 840
agccgacctg aatgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg 900
tttgtccggt tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg 960
tataatgcgg ctttgtttaa tcatcatcta ccacagagga acatgt atg ggt ggt 1015
Met Gly Gly
1
atc agt att tgg cag tta ttg att att gcc gtc atc gtt gta ctg ctt 1063
Ile Ser Ile Trp Gln Leu Leu Ile Ile Ala Val Ile Val Val Leu Leu
5 10 15
ttt ggc acc aaa aag ctc ggc tcc atc ggt tcc gat ctt ggt gcg tcg 1111
Phe Gly Thr Lys Lys Leu Gly Ser Ile Gly Ser Asp Leu Gly Ala Ser
20 25 30 35
atc aaa ggc ttt aaa aaa gca atg agc gat gat gaa cca aag cag gat 1159
Ile Lys Gly Phe Lys Lys Ala Met Ser Asp Asp Glu Pro Lys Gln Asp
40 45 50
aaa acc agc cag gat gct gat ttt act gcg aaa act atc gcc gat aag 1207
Lys Thr Ser Gln Asp Ala Asp Phe Thr Ala Lys Thr Ile Ala Asp Lys
55 60 65
cag gcg gat acg aat cag gaa cag gct aaa ata gaa gac gcg aag cgc 1255
Gln Ala Asp Thr Asn Gln Glu Gln Ala Lys Ile Glu Asp Ala Lys Arg
70 75 80
cac gat aaa gag cag gtg taa tct gtg ttt gat atc ggt ttt agc gaa 1303
His Asp Lys Glu Gln Val Val Phe Asp Ile Gly Phe Ser Glu
85 90 95
ctg cta ttg gtg ttc atc atc ggc ctc gtc gtt ctg ggg ccg caa cga 1351
Leu Leu Leu Val Phe Ile Ile Gly Leu Val Val Leu Gly Pro Gln Arg
100 105 110
ctg cct gtg gcg gta aaa acg gta gcg ggc tgg att cgc gcg ttg cgt 1399
Leu Pro Val Ala Val Lys Thr Val Ala Gly Trp Ile Arg Ala Leu Arg
115 120 125 130
tca ctg gcg aca acg gtg cag aac gaa ctg acc cag gag tta aaa ctc 1447
Ser Leu Ala Thr Thr Val Gln Asn Glu Leu Thr Gln Glu Leu Lys Leu
135 140 145
cag gag ttt cag gac agt ctg aaa aag gtt gaa aag gcg agc ctc act 1495
Gln Glu Phe Gln Asp Ser Leu Lys Lys Val Glu Lys Ala Ser Leu Thr
150 155 160
aac ctg acg ccc gaa ctg aaa gcg tcg atg gat gaa tta cgc cag gct 1543
Asn Leu Thr Pro Glu Leu Lys Ala Ser Met Asp Glu Leu Arg Gln Ala
165 170 175
gcg gag tcg atg aaa cgt tcc tac gtt gca aac gat cct gaa aag gcg 1591
Ala Glu Ser Met Lys Arg Ser Tyr Val Ala Asn Asp Pro Glu Lys Ala
180 185 190
agc gat gaa gcg cac acc atc cat aac ccg gtg gtg aaa gac aat gaa 1639
Ser Asp Glu Ala His Thr Ile His Asn Pro Val Val Lys Asp Asn Glu
195 200 205 210
act gcg cat gaa ggc gta acg cct gct gct gca caa acg cag gcc agt 1687
Thr Ala His Glu Gly Val Thr Pro Ala Ala Ala Gln Thr Gln Ala Ser
215 220 225
tcg ccg gaa cag aag cca gaa acc acg cca gag ccg gtg gta aaa cct 1735
Ser Pro Glu Gln Lys Pro Glu Thr Thr Pro Glu Pro Val Val Lys Pro
230 235 240
gct gcg gac gct gaa ccg aaa acc gct gca cct tcc cct tcg tcg agt 1783
Ala Ala Asp Ala Glu Pro Lys Thr Ala Ala Pro Ser Pro Ser Ser Ser
245 250 255
gat aaa ccg taaac atg tct gta gaa gat act caa ccg ctt atc acg cat 1833
Asp Lys Pro Met Ser Val Glu Asp Thr Gln Pro Leu Ile Thr His
260 265 270
ctg att gag ctg cgt aag cgt ctg ctg aac tgc att atc tcg gtg atc 1881
Leu Ile Glu Leu Arg Lys Arg Leu Leu Asn Cys Ile Ile Ser Val Ile
275 280 285
gtg ata ttc ctg tgt ctg gtc tat ttc gcc aat gac atc tat cac ctg 1929
Val Ile Phe Leu Cys Leu Val Tyr Phe Ala Asn Asp Ile Tyr His Leu
290 295 300 305
gta tcc gcg cca ctg atc aag cag ttg ccg caa ggt tca acg atg atc 1977
Val Ser Ala Pro Leu Ile Lys Gln Leu Pro Gln Gly Ser Thr Met Ile
310 315 320
gcc acc gac gtg gcc tcg ccg ttc ttt acg ccg atc aag ctg acc ttt 2025
Ala Thr Asp Val Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe
325 330 335
atg gtg tcg ctg att ctg tca gcg ccg gtg att ctc tat cag gtg tgg 2073
Met Val Ser Leu Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp
340 345 350
gcg ttt atc gcc cca gcg ctg tat aag cat gaa cgt cgc ctg gtg gtg 2121
Ala Phe Ile Ala Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val
355 360 365
ccg ctg ctg gtt tcc agc tct ctg ctg ttt tat atc ggc atg gcg ttc 2169
Pro Leu Leu Val Ser Ser Ser Leu Leu Phe Tyr Ile Gly Met Ala Phe
370 375 380 385
gcc tac ttt gtg gtc ttt ccg ctg gca ttt ggc ttc ctt gcc aat acc 2217
Ala Tyr Phe Val Val Phe Pro Leu Ala Phe Gly Phe Leu Ala Asn Thr
390 395 400
gcg ccg gaa ggg gta cag gta tcc acc gac atc gcg agc tat tta agc 2265
Ala Pro Glu Gly Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser
405 410 415
ttc gtt atg gcg ctg ttt atg gcg ttt ggt gtc tcc ttt gaa gtg ccg 2313
Phe Val Met Ala Leu Phe Met Ala Phe Gly Val Ser Phe Glu Val Pro
420 425 430
gtg gca att gtg ctg ctg tgc tgg atg ggg att acc tcg cca gaa gac 2361
Val Ala Ile Val Leu Leu Cys Trp Met Gly Ile Thr Ser Pro Glu Asp
435 440 445
tta cgc aaa aaa cgc ccg tat gtg ctg gtt ggt gca ttc gtt gtc ggg 2409
Leu Arg Lys Lys Arg Pro Tyr Val Leu Val Gly Ala Phe Val Val Gly
450 455 460 465
atg ttg ctg acg ccg ccg gat gtc ttc tcg caa acg ctg ttg gcg atc 2457
Met Leu Leu Thr Pro Pro Asp Val Phe Ser Gln Thr Leu Leu Ala Ile
470 475 480
cct atg tac tgc ctg ttt gaa atc ggt gtc ttc ttc tca cgc ttt tac 2505
Pro Met Tyr Cys Leu Phe Glu Ile Gly Val Phe Phe Ser Arg Phe Tyr
485 490 495
gtt ggt aaa ggg cga aac cgg gaa gag gaa aac gac gct gaa gca gaa 2553
Val Gly Lys Gly Arg Asn Arg Glu Glu Glu Asn Asp Ala Glu Ala Glu
500 505 510
agc gaa aaa act gaa gaa taa attcaaccgc ccgtcagggc ggttgtcat atg 2606
Ser Glu Lys Thr Glu Glu Met
515 520
gag tac agg atg ttt gat atc ggc gtt aat ttg acc agt tcg caa ttt 2654
Glu Tyr Arg Met Phe Asp Ile Gly Val Asn Leu Thr Ser Ser Gln Phe
525 530 535
gcg aaa gac cgt gat gat gtt gta gcg cgc gct ttt gac gcg gga gtt 2702
Ala Lys Asp Arg Asp Asp Val Val Ala Arg Ala Phe Asp Ala Gly Val
540 545 550
aat ggg cta ctc atc acc ggt acc aat ctg cgt gaa agc cag cag gcg 2750
Asn Gly Leu Leu Ile Thr Gly Thr Asn Leu Arg Glu Ser Gln Gln Ala
555 560 565
caa aag ctg gcg cgt cag tat tcg tcc tgt tgg tca acg gcg ggc gta 2798
Gln Lys Leu Ala Arg Gln Tyr Ser Ser Cys Trp Ser Thr Ala Gly Val
570 575 580 585
cat cct cac gac agc agc cag tgg caa gct gtg act gaa gaa gcg att 2846
His Pro His Asp Ser Ser Gln Trp Gln Ala Val Thr Glu Glu Ala Ile
590 595 600
att gag ctg gcc gcg cag cca gaa gtg gtg gcg att ggt gaa tgt ggt 2894
Ile Glu Leu Ala Ala Gln Pro Glu Val Val Ala Ile Gly Glu Cys Gly
605 610 615
ctc gac ttt aac cgc aac ttt tcg acg ccg gaa gag cag gaa cgc gct 2942
Leu Asp Phe Asn Arg Asn Phe Ser Thr Pro Glu Glu Gln Glu Arg Ala
620 625 630
ttt gtt gcc cag cta cgc att gcc gca gaa tta aac atg ccg gta ttt 2990
Phe Val Ala Gln Leu Arg Ile Ala Ala Glu Leu Asn Met Pro Val Phe
635 640 645
atg cac tgt cgc gat gcc cac gag cgg ttt atg aca ttg ctg gag ccg 3038
Met His Cys Arg Asp Ala His Glu Arg Phe Met Thr Leu Leu Glu Pro
650 655 660 665
tgg ctg gat aaa ctg cct ggt gcg gtt ctt cat tgc ttt acc ggc aca 3086
Trp Leu Asp Lys Leu Pro Gly Ala Val Leu His Cys Phe Thr Gly Thr
670 675 680
cgc gaa gag atg cag gcg tgc gtg gcg tgt gga att tat atc ggc att 3134
Arg Glu Glu Met Gln Ala Cys Val Ala Cys Gly Ile Tyr Ile Gly Ile
685 690 695
acc ggt tgg gtt tgc gat gaa cga cgc ggg ctg gag ctg cgg gaa ttg 3182
Thr Gly Trp Val Cys Asp Glu Arg Arg Gly Leu Glu Leu Arg Glu Leu
700 705 710
ttg ccg ttg att ccg gcg gag aaa ttg ctg atc gaa act gat gcg ccg 3230
Leu Pro Leu Ile Pro Ala Glu Lys Leu Leu Ile Glu Thr Asp Ala Pro
715 720 725
tat ctg ctc cct cgc gat ctc acg cca aag cca tca tcc cgg cgc aac 3278
Tyr Leu Leu Pro Arg Asp Leu Thr Pro Lys Pro Ser Ser Arg Arg Asn
730 735 740 745
gag cca gcc cat ctg ccc cat att ttg caa cgt att gcg cac tgg cgt 3326
Glu Pro Ala His Leu Pro His Ile Leu Gln Arg Ile Ala His Trp Arg
750 755 760
gga gaa gat gcc gca tgg ctg gct gcc acc acg gat gcc aat gtc aaa 3374
Gly Glu Asp Ala Ala Trp Leu Ala Ala Thr Thr Asp Ala Asn Val Lys
765 770 775
aca ctg ttt ggg att gcg ttt tag agtttgcg 3406
Thr Leu Phe Gly Ile Ala Phe
780 785
<210>11
<211>89
<212>PRT
<213>大肠杆菌
<400>11
Met Gly Gly Ile Ser Ile Trp Gln Leu Leu Ile Ile Ala Val Ile Val
1 5 10 15
Val Leu Leu Phe Gly Thr Lys Lys Leu Gly Ser Ile Gly Ser Asp Leu
20 25 30
Gly Ala Ser Ile Lys Gly Phe Lys Lys Ala Met Ser Asp Asp Glu Pro
35 40 45
Lys Gln Asp Lys Thr Ser Gln Asp Ala Asp Phe Thr Ala Lys Thr Ile
50 55 60
Ala Asp Lys Gln Ala Asp Thr Asn Gln Glu Gln Ala Lys Ile Glu Asp
65 70 75 80
Ala Lys Arg His Asp Lys Glu Gln Val
85
<210>12
<211>171
<212>PRT
<213>大肠杆菌
<400>12
Val Phe Asp Ile Gly Phe Ser Glu Leu Leu Leu Val Phe Ile Ile Gly
1 5 10 15
Leu Val Val Leu Gly Pro Gln Arg Leu Pro Val Ala Val Lys Thr Val
20 25 30
Ala Gly Trp Ile Arg Ala Leu Arg Ser Leu Ala Thr Thr Val Gln Asn
35 40 45
Glu Leu Thr Gln Glu Leu Lys Leu Gln Glu Phe Gln Asp Ser Leu Lys
50 55 60
Lys Val Glu Lys Ala Ser Leu Thr Asn Leu Thr Pro Glu Leu Lys Ala
65 70 75 80
Ser Met Asp Glu Leu Arg Gln Ala Ala Glu Ser Met Lys Arg Ser Tyr
85 90 95
Val Ala Asn Asp Pro Glu Lys Ala Ser Asp Glu Ala His Thr Ile His
100 105 110
Asn Pro Val Val Lys Asp Asn Glu Thr Ala His Glu Gly Val Thr Pro
115 120 125
Ala Ala Ala Gln Thr Gln Ala Ser Ser Pro Glu Gln Lys Pro Glu Thr
130 135 140
Thr Pro Glu Pro Val Val Lys Pro Ala Ala Asp Ala Glu Pro Lys Thr
145 150 155 160
Ala Ala Pro Ser Pro Ser Ser Ser Asp Lys Pro
165 170
<210>13
<211>258
<212>PRT
<213>大肠杆菌
<400>13
Met Ser Val Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu
1 5 10 15
Arg Lys Arg Leu Leu Asn Cys Ile Ile Ser Val Ile Val Ile Phe Leu
20 25 30
Cys Leu Val Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ser Ala Pro
35 40 45
Leu Ile Lys Gln Leu Pro Gln Gly Ser Thr Met Ile Ala Thr Asp Val
50 55 60
Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu
65 70 75 80
Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala
85 90 95
Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val
100 105 110
Ser Ser Ser Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val
115 120 125
Val Phe Pro Leu Ala Phe Gly Phe Leu Ala Asn Thr Ala Pro Glu Gly
130 135 140
Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala
145 150 155 160
Leu Phe Met Ala Phe Gly Val Ser Phe Glu Val Pro Val Ala Ile Val
165 170 175
Leu Leu Cys Trp Met Gly Ile Thr Ser Pro Glu Asp Leu Arg Lys Lys
180 185 190
Arg Pro Tyr Val Leu Val Gly Ala Phe Val Val Gly Met Leu Leu Thr
195 200 205
Pro Pro Asp Val Phe Ser Gln Thr Leu Leu Ala Ile Pro Met Tyr Cys
210 215 220
Leu Phe Glu Ile Gly Val Phe Phe Ser Arg Phe Tyr Val Gly Lys Gly
225 230 235 240
Arg Asn Arg Glu Glu Glu Asn Asp Ala Glu Ala Glu Ser Glu Lys Thr
245 250 255
Glu Glu
<210>14
<211>264
<212>PRT
<213>大肠肠菌
<400>14
Met Glu Tyr Arg Met Phe Asp Ile Gly Val Asn Leu Thr Ser Ser Gln
1 5 10 15
Phe Ala Lys Asp Arg Asp Asp Val Val Ala Arg Ala Phe Asp Ala Gly
20 25 30
Val Asn Gly Leu Leu Ile Thr Gly Thr Asn Leu Arg Glu Ser Gln Gln
35 40 45
Ala Gln Lys Leu Ala Arg Gln Tyr Ser Ser Cys Trp Ser Thr Ala Gly
50 55 60
Val His Pro His Asp Ser Ser Gln Trp Gln Ala Val Thr Glu Glu Ala
65 70 75 80
Ile Ile Glu Leu Ala Ala Gln Pro Glu Val Val Ala Ile Gly Glu Cys
85 90 95
Gly Leu Asp Phe Asn Arg Asn Phe Ser Thr Pro Glu Glu Gln Glu Arg
100 105 110
Ala Phe Val Ala Gln Leu Arg Ile Ala Ala Glu Leu Asn Met Pro Val
115 120 125
Phe Met His Cys Arg Asp Ala His Glu Arg Phe Met Thr Leu Leu Glu
130 135 140
Pro Trp Leu Asp Lys Leu Pro Gly Ala Val Leu His Cys Phe Thr Gly
145 150 155 160
Thr Arg Glu Glu Met Gln Ala Cys Val Ala Cys Gly Ile Tyr Ile Gly
165 170 175
Ile Thr Gly Trp Val Cys Asp Glu Arg Arg Gly Leu Glu Leu Arg Glu
180 185 190
Leu Leu Pro Leu Ile Pro Ala Glu Lys Leu Leu Ile Glu Thr Asp Ala
195 200 205
Pro Tyr Leu Leu Pro Arg Asp Leu Thr Pro Lys Pro Ser Ser Arg Arg
210 215 220
Asn Glu Pro Ala His Leu Pro His Ile Leu Gln Arg Ile Ala His Trp
225 230 235 240
Arg Gly Glu Asp Ala Ala Trp Leu Ala Ala Thr Thr Asp Ala Asn Val
245 250 255
Lys Thr Leu Phe Gly Ile Ala Phe
260
<210>15
<211>586
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(170)..(370)
<400>15
tcttaaacaa ccgtcgcttt gcgccgccgc aattattatg atgttttttt actcggcgct 60
tgattcacct tgttacagat tgctattgtg tgcgcgcgtc gaatgaccgt taatattctc 120
tggtttttaa ggcgcgttct gttgccggtt atatgtcaag aaggtatct atg ggt gag 178
Met Gly Glu
1
att agt att acc aaa ctg ctg gta gtt gcg gcg ctg gtc gtt ctg ctg 226
Ile Ser Ile Thr Lys Leu Leu Val Val Ala Ala Leu Val Val Leu Leu
5 10 15
ttt ggg act aag aag tta cgt acg ctg ggc gga gac ctt gga gcg gcc 274
Phe Gly Thr Lys Lys Leu Arg Thr Leu Gly Gly Asp Leu Gly Ala Ala
20 25 30 35
att aaa ggg ttc aag aag gcg atg aat gat gac gat gct gcg gcg aaa 322
Ile Lys Gly Phe Lys Lys Ala Met Asn Asp Asp Asp Ala Ala Ala Lys
40 45 50
aaa ggc gca gac gtt gat ctt cag gct gaa aag ctc tct cat aaa gag 370
Lys Gly Ala Asp Val Asp Leu Gln Ala Glu Lys Leu Ser His Lys Glu
55 60 65
tgacgtggcg agcaggacgc tccctcaata tcttgttcga tacaaaaacc cgcttcaaaa 430
agcgggtttt ttatcagaca gatgtaagta attattacag gattacttaa cttccatccc 490
tttcgcctgc aaatcggcgt ggtaagaaga gcggacaaac ggaccgcatg cagcatgggt 550
aaagcccatc gccagcgctt cgctttcatt tcgtcg 586
<210>16
<211>67
<212>PRT
<213>大肠杆菌
<400>16
Met Gly Glu Ile Ser Ile Thr Lys Leu Leu Val Val Ala Ala Leu Val
1 5 10 15
Val Leu Leu Phe Gly Thr Lys Lys Leu Arg Thr Leu Gly Gly Asp Leu
20 25 30
Gly Ala Ala Ile Lys Gly Phe Lys Lys Ala Met Asn Asp Asp Asp Ala
35 40 45
Ala Ala Lys Lys Gly Ala Asp Val Asp Leu Gln Ala Glu Lys Leu Ser
50 55 60
His Lys Glu
65
<210>17
<211>4200
<212>DNA
<213>鼠伤寒沙门氏菌
<220>
<221>CDS
<222>(947)..(1444)
<220>
<221>CDS
<222>(1450)..(1722)
<400>17
cgcaagtcaa tgtcgtcccg gtcgtatgta aaagtatgtg aatagggcgg gcgaaagcgg 60
ctaacaaaga ggcagcgtga aggataatgt gtataatgcg gccctaataa ttcatcatct 120
atcacagagg aacatgtatg ggtggtatca gtatttggca gttgttgatt gttgccgtta 180
tcgtcgtact gctgttcggc accaaaaaac tcggttccat cggttccgat cttggcgcgt 240
ctatcaaagg ctttaaaaag gccatgagcg atgatgatgc caaacaggat aaaaccagtc 300
aggacgctga ttttaccgct aaatctatcg cggataagca aggcgaagcg aaaaaggaag 360
acgctaaaag ccaagataaa gagcaggtat aatccgtgtt tgatatcggt tttagcgaac 420
tgctgttagt gttcgttatc ggcctcattg tgttggggcc gcaacgattg ccagtagcgg 480
taaaaacggt agcgggctgg attcgcgcgt tgcggtccct tgcgacaacg gttcagaatg 540
aactgactca ggaactgaaa cttcaggagt tccaggacag tctgaaaaaa gtcgaaaagg 600
cgagcctgga aaatctgact cccgaactga aagcatctat ggatgaactg cgtcaggcgg 660
cggagtcgat gaaacgcacc tacagcgcta acgatcccga acaagcgagc gatgaagcgc 720
ataccatcca taatccggtg gtaaaaggga acgaaacgca gcatgagggc gtcacccctg 780
ccgccgctga aacacaggcg agcgcgccgg aacaaaagcc ggagcccgtt aaagctaacg 840
tgcctgagtc gacggaaacc gcttccgtag ccacgataga cgccgagaag aaatccgctg 900
cgcctgttgt cgaatcttcc ccctcgtcga gtgataaacc gtaaac atg gct gta 955
Met Ala Val
1
gaa gat act caa ccg ctt atc acg cat ctg atc gag ttg cgt aag cgc 1003
Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu Arg Lys Arg
5 10 15
ctg cta aac tgc atc gtc gca gta ctt ctg att ttt ctg gcg tta att 1051
Leu Leu Asn Cys Ile Val Ala Val Leu Leu Ile Phe Leu Ala Leu Ile
20 25 30 35
tat ttc gcc aat gat att tat cat tta gtc gcc gca ccg ctg att aaa 1099
Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ala Ala Pro Leu Ile Lys
40 45 50
cag atg ccg caa ggg gcg aca atg att gcg acg gat gtg gcg tcg ccg 1147
Gln Met Pro Gln Gly Ala Thr Met Ile Ala Thr Asp Val Ala Ser Pro
55 60 65
ttt ttt acg cct atc aaa ctc acc ttc atg gtg tct ttg atc tta tcc 1195
Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu Ile Leu Ser
70 75 80
gcg cct gtc att ttg tac cag gtt tgg gcc ttt atc gcc ccg gcg ctg 1243
Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala Pro Ala Leu
85 90 95
tat aag cat gag cgt cgt ctg gtc gta cct ctg ctg gta tcc agc tcg 1291
Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val Ser Ser Ser
100 105 110 115
ctg ctt ttc tat att ggt atg gcc ttc gcc tat ttt gtc gta ttc cct 1339
Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val Val Phe Pro
120 125 130
ttg gcc ttt ggt ttc ctg acg cat acg gcg ccg gaa ggg gta cag gtt 1387
Leu Ala Phe Gly Phe Leu Thr His Thr Ala Pro Glu Gly Val Gln Val
135 140 145
tcg aca gat atc gcc agc tat ctt agc ttt gtc atg gcg ctt ttt atg 1435
Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala Leu Phe Met
150 155 160
gcc ttt gcg tagcc ttt gaa gtg ccg gtg gcg att gtg ttg ctg tgc tgg 1485
Ala Phe Ala Phe Glu Val Pro Val Ala Ile Val Leu Leu Cys Trp
165 170 175
atg ggc atc acc acg cca gaa gat ttg cgt aaa aaa cgg cct tat atc 1533
Met Gly Ile Thr Thr Pro Glu Asp Leu Arg Lys Lys Arg Pro Tyr Ile
180 185 190
ctg gtc ggg gca ttc att gtg gga atg ctg ctt acg ccg cca gat gtt 1581
Leu Val Gly Ala Phe Ile Val Gly Met Leu Leu Thr Pro Pro Asp Val
195 200 205 210
ttc tcg caa acg ttg ctg gcg ata ccg atg tac tgc ctg ttt gaa att 1629
Phe Ser Gln Thr Leu Leu Ala Ile Pro Met Tyr Cys Leu Phe Glu Ile
215 220 225
ggc gtt ttc tgc tca cgc ttt tat gtc ggt aag cga cgg acg cgc gac 1677
Gly Val Phe Cys Ser Arg Phe Tyr Val Gly Lys Arg Arg Thr Arg Asp
230 235 240
gaa gat aac gag gcc gaa acc gaa aag gcc gag cac act gaa gac 1722
Glu Asp Asn Glu Ala Glu Thr Glu Lys Ala Glu His Thr Glu Asp
245 250 255
taaacacaac cgcccgccag ggcggttgtc atatgggggc aagcatgttt gatattggcg 1782
ttaatttaac cagtagccag tttgcaaaag atcgtgatga tgtggtcgcc cgtgcgtttg 1842
cggcgggagt aaaaggtatg ctactgaccg gaacgaacat ccatgaaagt cagcaggcgt 1902
taaaactggc gcggcgctac ccccattgtt ggtcgacggc tggcgtccat ccccatgaca 1962
gcagtcagtg gtcacccgcg tctgaagacg ccattattgc gctggcgaac cagccggaag 2022
tcgtcgctat cggtgagtgc gggctggatt tcaatcgcaa tttttccacg ccgcaggagc 2082
aggagcgtgc ctttcaggcg cagctacaaa ttgccgccga attgcagata ccaatcttta 2142
tgcactgccg ggacgcgcat gagcgatttc tggtattgct tgatccctgg ctggatagtc 2202
ttcctggtgc aatactgcac tgctttaccg gttcacgcca gcaaatgcag gcctgtgtgg 2262
atagagggct ctatatcggt attaccgggt gggtttgcga cgaacgacgc gggcttgagc 2322
tacgtgaact cttaccgttt attccagcgg aaaagctact gatagaaacc gacgcgccgt 2382
atctgttgcc tcgcgatctt acgccgaaac caacgtcacg acgcaacgag cccgcgtatc 2442
tgcctcacat cctggagcgc atagcgctat ggcgtggtga agatccgcaa tggttagcgg 2502
cgatgacaga tgccaacgcc agaaccttat ttgaggttgt attctgaacg atcgctaaat 2562
cttgcgaaaa ccggtgtttt ttacgctctg cttcacttct ttattgagta aattaagcag 2622
taacatcgaa cgcgtttcgc catccggttc ggtaaaaatc gctttcagcc cttcaaatgc 2682
gccttccgtg atgatgacgc tatcgccggg atagggggtt tcaggatcga caacgccttc 2742
gggcttgtag atagaaagct gatgaataac gctggaaggc acgatcgcag gatgccgcca 2802
aagcgcacaa aatggctgac gccgcgcgtg gcgttgattg tagtggtatg tatcacttcc 2862
ggatcaaatt caacgaacag ataattagga aagagcggtt cgctgacgga ggtacgtttt 2922
ccgcgtacca ttttttccag ggtgatcatc ggtgtcaggc aacttaccgc ttgtctttcg 2982
aggtgttcct gagcacgctg aagttgcccg cgtttgcagt acagtaaata ccaggattgc 3042
ataatgactc ttatccgctt gttcggggcg caagcatagc aaaagccatg cgcgaagtta 3102
attatacact tcatccttta agccgtatct ggattagcgt tggttgccag agttcacgct 3162
aatttaacaa aaatacagca tcccgatgat gaacgccgta taatgatgcg cttaccaaga 3222
ggctacaatg gacgccatga aatatcacga tttacgcgac ttcctgacgc tacttgagca 3282
gcagggggaa ctaaaacgca tcacgctacc tgtggatcct catctggaaa tcacggaaat 3342
cgctgaccgc acgctgcgtg ccggtggacc ggcgttgctg tttgaaaatc ctaaaggtta 3402
cgccatgccg gtgctgtgca acctttttgg cacgccaaaa cgcgtggcga tgggcatggg 3462
gcaggatgat gtttccgcct tacgggaagt gggtaaatta ttagcgtttc ttaaagaacc 3522
tgagccgccg aaagcgtttc gcgatctgtt tgacaagctg ccgcagttta agcaagtgct 3582
gaatatgccg acgaaacggt tacgcggcgc gccttgccag cagaaaatcg cgtctggcga 3642
tgatgtcgat ttaacgcgtc ttcctgtcat gacctgttgg ccggacgacg ccgcgccgct 3702
gattacctgg ggactgacgg taacgcgtgg tccgcacaaa gagcggcaaa acctgggcat 3762
ttatcgtcag cagttgatag gtaaaaataa gctgattatg cgctggctgt ctcaccgcgg 3822
cggcgcgctg gattttcagg agtggttagc cgcgcgtccg ggtgaacgtt tcccggtctc 3882
cgtcgcattg ggcgccgatc cggcacgata cttggcgccg tgactcctgt tcccgatact 3942
ctgtcggagt atgcctttgc gggcctgctg cgcggcacga aaactgaagt ggttaatgct 4002
ttctacgatc tggagtgctg cagcgcgaga tatcttgaag tacatgagcg gagagatgcg 4062
cggagacgta tgcgatcata cggcatatat gagtgatagc tcgtcttacg tcacgcaata 4122
acagcgtaga tgcatctata tcactatacg cgcgcatgag ctcgtatagg tgcctcatat 4182
ctcgtctatc tcaaagtc 4200
<210>18
<211>166
<212>PRT
<213>鼠伤寒沙门氏菌
<400>18
Met Ala Val Glu Asp Thr Gln Pro Leu Ile Thr His Leu Ile Glu Leu
1 5 10 15
Arg Lys Arg Leu Leu Asn Cys Ile Val Ala Val Leu Leu Ile Phe Leu
20 25 30
Ala Leu Ile Tyr Phe Ala Asn Asp Ile Tyr His Leu Val Ala Ala Pro
35 40 45
Leu Ile Lys Gln Met Pro Gln Gly Ala Thr Met Ile Ala Thr Asp Val
50 55 60
Ala Ser Pro Phe Phe Thr Pro Ile Lys Leu Thr Phe Met Val Ser Leu
65 70 75 80
Ile Leu Ser Ala Pro Val Ile Leu Tyr Gln Val Trp Ala Phe Ile Ala
85 90 95
Pro Ala Leu Tyr Lys His Glu Arg Arg Leu Val Val Pro Leu Leu Val
100 105 110
Ser Ser Ser Leu Leu Phe Tyr Ile Gly Met Ala Phe Ala Tyr Phe Val
115 120 125
Val Phe Pro Leu Ala Phe Gly Phe Leu Thr His Thr Ala Pro Glu Gly
130 135 140
Val Gln Val Ser Thr Asp Ile Ala Ser Tyr Leu Ser Phe Val Met Ala
145 150 155 160
Leu Phe Met Ala Phe Ala
165
<210>19
<211>91
<212>PRT
<213>鼠伤寒沙门氏菌
<400>19
Phe Glu Val Pro Val Ala Ile Val Leu Leu Cys Trp Met Gly Ile Thr
1 5 10 15
Thr Pro Glu Asp Leu Arg Lys Lys Arg Pro Tyr Ile Leu Val Gly Ala
20 25 30
Phe Ile Val Gly Met Leu Leu Thr Pro Pro Asp Val Phe Ser Gln Thr
35 40 45
Leu Leu Ala Ile Pro Met Tyr Cys Leu Phe Glu Ile Gly Val Phe Cys
50 55 60
Ser Arg Phe Tyr Val Gly Lys Arg Arg Thr Arg Asp Glu Asp Asn Glu
65 70 75 80
Ala Glu Thr Glu Lys Ala Glu His Thr Glu Asp
85 90
<210>20
<211>2601
<212>DNA
<213>脑膜炎奈瑟氏球菌
<220>
<221>CDS
<222>(1572)..(2339)
<400>20
agacaaaatc ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga 60
caaaaacccg tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct 120
tctgacacac cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgccaagg 180
tttgtcgggc ttggccgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg 240
cactttccca cattcaatcc gtctgaccgc tgttcagacg gcatcggagc cgttatggac 300
aactgtattt tctgcaaaat cgccgccaaa gacattccgg cgcaaaccgt ctatgaagac 360
ggcgaaatgg tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt 420
cccaaagtcc atttcgattc gttggcacac gccgcgcccg aacatcagcc ccttttggga 480
aaaatgatgc tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa 540
accctgatca acaccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc 600
atgggcacac ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata 660
catcatgggc agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat 720
attcggcacc aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt 780
caaacagggg ctgaacgaag gtacagacgg caaagaagcc caaaaagacg atgtaatcga 840
acacaaaaaa gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt 900
ttttgtcggc attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg 960
caccgccgga cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt 1020
tgacacgcaa atcgaactgg aagaactaag gaaggcaaag caggaatttg aagctgccgc 1080
tgctcaggtt cgagacagcc tcaaagaaac cggtacggat atggagggta atctgcacga 1140
catttccgac ggtctgaagc cttgggaaaa actgcccgaa cagcgcacgc ctgctgattt 1200
cggtgtcgat gaaaacggca atccctttcc cgatgcggca aacaccctat tagacggcat 1260
ttccgacgtt atgccgtccg aacgttccta cgcttccgcc gaaacccttg gggacagcgg 1320
gcaaaccggc agtacagccg aacccgcgga aaccgaccaa gaccgtgcat ggcgggaata 1380
cctgactgct tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac 1440
cgctgttgaa acccctgttc cgcataccac ttcgctgcgt aaacaggcaa taagccgcaa 1500
acgcgatttg cgtcctaaat cccgcgccaa acctaaattg cgcgtccgta aatcataaag 1560
agggcaatcc g gtg tcc gaa aca caa aac gaa caa ccc gtc caa ccg ctt 1610
Val Ser Glu Thr Gln Asn Glu Gln Pro Val Gln Pro Leu
1 5 10
gtc gag cat ctc atc gag ctg cgc cgc cgc ctg atg tgg acg gtt gtc 1658
Val Glu His Leu Ile Glu Leu Arg Arg Arg Leu Met Trp Thr Val Val
15 20 25
ggt atc tta gtc tgc ttt ttc ggc cta atg ccg ttt gcc caa caa ctc 1706
Gly Ile Leu Val Cys Phe Phe Gly Leu Met Pro Phe Ala Gln Gln Leu
30 35 40 45
tat act ttt atc gcc gac ccg ctg atg gca aac ctg ccc aaa gac acc 1754
Tyr Thr Phe Ile Ala Asp Pro Leu Met Ala Asn Leu Pro Lys Asp Thr
50 55 60
agc atg att gcc acc gat gtc atc gca cca ttt ttc gtg ccg gtc aaa 1802
Ser Met Ile Ala Thr Asp Val Ile Ala Pro Phe Phe Val Pro Val Lys
65 70 75
gtt acc ctg atg gcg gca ttt tta att tcg ctg ccg cat acg ctc tac 1850
Val Thr Leu Met Ala Ala Phe Leu Ile Ser Leu Pro His Thr Leu Tyr
80 85 90
caa atc tgg gca ttc gtc gcc ccc gca ctc tac caa aac gaa aaa cgc 1898
Gln Ile Trp Ala Phe Val Ala Pro Ala Leu Tyr Gln Asn Glu Lys Arg
95 100 105
ctg att acg ccg ctc gtc ctc tcc agc gtc agc ctg ttt ttc atc ggc 1946
Leu Ile Thr Pro Leu Val Leu Ser Ser Val Ser Leu Phe Phe Ile Gly
110 115 120 125
atg gca ttt gcc tac ttt ttg gtt ttc ccc gtc att ttc aaa ttc ctt 1994
Met Ala Phe Ala Tyr Phe Leu Val Phe Pro Val Ile Phe Lys Phe Leu
130 135 140
gcc agc gtt acc cct gtc ggt gtc aat atg gcg aca gac atc gac aaa 2042
Ala Ser Val Thr Pro Val Gly Val Asn Met Ala Thr Asp Ile Asp Lys
145 150 155
tac ctc tcc ttc atc ttg ggg atg ttt gtc gca ttc ggt aca acg ttt 2090
Tyr Leu Ser Phe Ile Leu Gly Met Phe Val Ala Phe Gly Thr Thr Phe
160 165 170
gaa gtc ccc att gtc gtt atc ctg tta acc aaa att ggt gtg gta aca 2138
Glu Val Pro Ile Val Val Ile Leu Leu Thr Lys Ile Gly Val Val Thr
175 180 185
acc gaa cag ctc aaa cgc gcc cgc ccc tat gtg att gtc ggc gcg ttt 2186
Thr Glu Gln Leu Lys Arg Ala Arg Pro Tyr Val Ile Val Gly Ala Phe
190 195 200 205
gtc att gcc gcc atc atc acg ccg ccc gat gtg att tca caa acc ctg 2234
Val Ile Ala Ala Ile Ile Thr Pro Pro Asp Val Ile Ser Gln Thr Leu
210 215 220
ctt gcc att ccg ctg att ctc tta tac gaa gca ggt att tgg ttc gga 2282
Leu Ala Ile Pro Leu Ile Leu Leu Tyr Glu Ala Gly Ile Trp Phe Gly
225 230 235
cgc ttt ttc acg cca cgt tca gaa cag gat ggc gac ata cag ccg cct 2330
Arg Phe Phe Thr Pro Arg Ser Glu Gln Asp Gly Asp Ile Gln Pro Pro
240 245 250
gca aca acc tgacactatg ccgtccgaac ctccgcctca taccgccaca 2379
Ala Thr Thr
255
gattaaggaa tacctttgaa taccctctat ttaggttcaa acagcccgcg ccgaatggaa 2439
atcctgacac agttgggcta tcaggtcgtc aagctgcctg ccaacatcga cgaaacggtc 2499
agacagaacg aagaccctgc ccgttacgtt caaaggatgg cagaagaaaa aaaccgaacc 2559
gccctgaccc tcttttgcga aaccaacggc acaatgcccg at 2601
<210>21
<211>256
<212>PRT
<213>脑膜炎奈瑟氏球菌
<400>21
Val Ser Glu Thr Gln Asn Glu Gln Pro Val Gln Pro Leu Val Glu His
1 5 10 15
Leu Ile Glu Leu Arg Arg Arg Leu Met Trp Thr Val Val Gly Ile Leu
20 25 30
Val Cys Phe Phe Gly Leu Met Pro Phe Ala Gln Gln Leu Tyr Thr Phe
35 40 45
Ile Ala Asp Pro Leu Met Ala Asn Leu Pro Lys Asp Thr Ser Met Ile
50 55 60
Ala Thr Asp Val Ile Ala Pro Phe Phe Val Pro Val Lys Val Thr Leu
65 70 75 80
Met Ala Ala Phe Leu Ile Ser Leu Pro His Thr Leu Tyr Gln Ile Trp
85 90 95
Ala Phe Val Ala Pro Ala Leu Tyr Gln Asn Glu Lys Arg Leu Ile Thr
100 105 110
Pro Leu Val Leu Ser Ser Val Ser Leu Phe Phe Ile Gly Met Ala Phe
115 120 125
Ala Tyr Phe Leu Val Phe Pro Val Ile Phe Lys Phe Leu Ala Ser Val
130 135 140
Thr Pro Val Gly Val Asn Met Ala Thr Asp Ile Asp Lys Tyr Leu Ser
145 150 155 160
Phe Ile Leu Gly Met Phe Val Ala Phe Gly Thr Thr Phe Glu Val Pro
165 170 175
Ile Val Val Ile Leu Leu Thr Lys Ile Gly Val Val Thr Thr Glu Gln
180 185 190
Leu Lys Arg Ala Arg Pro Tyr Val Ile Val Gly Ala Phe Val Ile Ala
195 200 205
Ala Ile Ile Thr Pro Pro Asp Val Ile Ser Gln Thr Leu Leu Ala Ile
210 215 220
Pro Leu Ile Leu Leu Tyr Glu Ala Gly Ile Trp Phe Gly Arg Phe Phe
225 230 235 240
Thr Pro Arg Ser Glu Gln Asp Gly Asp Ile Gln Pro Pro Ala Thr Thr
245 250 255
<210>22
<211>4604
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(2982)..(4082)
<220>
<221>CDS
<222>(1534)..(2637)
<220>
<221>CDS
<222>(749)..(1531)
<220>
<221>CDS
<222>(6)..(746)
<400>22
ggcta gtt gat gat aat ttg aaa ggt caa ggt gca gga aaa aat ttt tta 50
Val Asp Asp Asn Leu Lys Gly Gln Gly Ala Gly Lys Asn Phe Leu
1 5 10 15
tcg ctg ata aag tac agc gag aca gat tat aca att tat tgt gac caa 98
Set Leu Ile Lys Tyr Ser Glu Thr Asp Tyr Thr Ile Tyr Cys Asp Gln
20 25 30
gat gat att tgg tta gaa aac aaa ata ttt gaa tta gta aag tat gca 146
Asp Asp Ile Trp Leu Glu Asn Lys Ile Phe Glu Leu Val Lys Tyr Ala
35 40 45
aat gaa att aaa ttg aat gta tca gat gcg cct tcg cta gtt tat gct 194
Asn Glu Ile Lys Leu Asn Val Ser Asp Ala Pro Ser Leu Val Tyr Ala
50 55 60
gat ggc tat gct tat atg gat ggt gag ggt aca atc gat ttt tct ggg 242
Asp Gly Tyr Ala Tyr Met Asp Gly Glu Gly Thr Ile Asp Phe Ser Gly
65 70 75
ata tct aac aat cat gct gat caa tta aag gat ttt ctt ttt ttt aat 290
Ile Ser Asn Asn His Ala Asp Gln Leu Lys Asp Phe Leu Phe Phe Asn
80 85 90 95
ggt gga tac caa gga tgt tct att atg ttc aat cgt gca atg acc aaa 338
Gly Gly Tyr Gln Gly Cys Ser Ile Met Phe Asn Arg Ala Met Thr Lys
100 105 110
ttt ctt ctg aat tat cga gga ttt gta tat cta cat gac gat atc aca 386
Phe Leu Leu Asn Tyr Arg Gly Phe Val Tyr Leu His Asp Asp Ile Thr
115 120 125
aca tta gct gca tac gct ctt ggt aaa gtt tat ttt ctc ccg aaa tac 434
Thr Leu Ala Ala Tyr Ala Leu Gly Lys Val Tyr Phe Leu Pro Lys Tyr
130 135 140
ctt atg tta tat aga cag cac acg aat gcg gta act ggt atc aaa aca 482
Leu Met Leu Tyr Arg Gln His Thr Asn Ala Val Thr Gly Ile Lys Thr
145 150 155
ttc cgc aat gga ttg act tct aaa ttt aaa tca cca gta aac tat ctt 530
Phe Arg Asn Gly Leu Thr Ser Lys Phe Lys Ser Pro Val Asn Tyr Leu
160 165 170 175
tta tca cga aaa cat tat cag gta aaa aaa tct ttt ttt gaa tgt aac 578
Leu Ser Arg Lys His Tyr Gln Val Lys Lys Ser Phe Phe Glu Cys Asn
180 185 190
agc tct atc tta tca gag acg aat aaa aaa gtt ttt ttg gat ttt att 626
Ser Ser Ile Leu Ser Glu Thr Asn Lys Lys Val Phe Leu Asp Phe Ile
195 200 205
tca ttt tgt gaa tca aat aat aaa ttt aca gat ttt ttt aag tta tgg 674
Ser Phe Cys Glu Ser Asn Asn Lys Phe Thr Asp Phe Phe Lys Leu Trp
210 215 220
cga ggt ggg ttt aga tta aat aac agt aga act aaa tta tta tta aaa 722
Arg Gly Gly Phe Arg Leu Asn Asn Ser Arg Thr Lys Leu Leu Leu Lys
225 230 235
ttc tta ata cgg aga aaa ttt agc ga atg att tca ata ctt aca cct 769
Phe Leu Ile Arg Arg Lys Phe Ser Met Ile Ser Ile Leu Thr Pro
240 245 250
act ttt aat cgg caa cat act tta tca agg cta ttc aat tct ctt ata 817
Thr Phe Asn Arg Gln His Thr Leu Ser Arg Leu Phe Asn Ser Leu Ile
255 260 265 270
tta caa act gat aaa gat ttt gag tgg ata ata att gat gat ggt agt 865
Leu Gln Thr Asp Lys Asp Phe Glu Trp Ile Ile Ile Asp Asp Gly Ser
275 280 285
ata gat gca aca gcg gta ctt gta gaa gat ttt aga aaa aaa tgt gat 913
Ile Asp Ala Thr Ala Val Leu Val Glu Asp Phe Arg Lys Lys Cys Asp
290 295 300
ttt gac ttg att tat tgc tat cag gaa aat aat ggt aag ccc atg gct 961
Phe Asp Leu Ile Tyr Cys Tyr Gln Glu Asn Asn Gly Lys Pro Met Ala
305 310 315
tta aac gct ggt gtt aaa gct tgt aga ggc gat tat atc ttt att gtt 1009
Leu Asn Ala Gly Val Lys Ala Cys Arg Gly Asp Tyr Ile Phe Ile Val
320 325 330
gac agt gat gat gca cta act ccc gat gcc ata aaa tta att aaa gaa 1057
Asp Ser Asp Asp Ala Leu Thr Pro Asp Ala Ile Lys Leu Ile Lys Glu
335 340 345 350
tca ata cat gat tgc tta tct gag aag gaa agt ttc agc gga gtc ggt 1105
Ser Ile His Asp Cys Leu Ser Glu Lys Glu Ser Phe Ser Gly Val Gly
355 360 365
ttt aga aaa gca tat ata aaa ggg ggg att att ggt aat gat tta aat 1153
Phe Arg Lys Ala Tyr Ile Lys Gly Gly Ile Ile Gly Asn Asp Leu Asn
370 375 380
aat tct tca gaa cat ata tac tat tta aat gcg act gag att agc aat 1201
Asn Ser Ser Glu His Ile Tyr Tyr Leu Asn Ala Thr Glu Ile Ser Asn
385 390 395
tta ata aat ggt gat gtt gca tat tgt ttt aaa aaa gaa agt ttg gta 1249
Leu Ile Asn Gly Asp Val Ala Tyr Cys Phe Lys Lys Glu Ser Leu Val
400 405 410
aaa aat cca ttc ccc cgt ata gaa gat gaa aaa ttt gtt cca gaa tta 1297
Lys Asn Pro Phe Pro Arg Ile Glu Asp Glu Lys Phe Val Pro Glu Leu
415 420 425 430
tat att tgg aat aaa ata act gac aag gcg aag att cga ttt aac ata 1345
Tyr Ile Trp Asn Lys Ile Thr Asp Lys Ala Lys Ile Arg Phe Asn Ile
435 440 445
agc aaa gtt ata tat ctt tgt gag tat ctt gat gat ggt ctt tct aaa 1393
Ser Lys Val Ile Tyr Leu Cys Glu Tyr Leu Asp Asp Gly Leu Ser Lys
450 455 460
aat ttc cat aac cag ctt aaa aaa tac cca aag ggg ttt aag att tat 1441
Asn Phe His Asn Gln Leu Lys Lys Tyr Pro Lys Gly Phe Lys Ile Tyr
465 470 475
tac aaa gat caa aga aaa cga gag aaa act tat ata aaa aaa aca aag 1489
Tyr Lys Asp Gln Arg Lys Arg Glu Lys Thr Tyr Ile Lys Lys Thr Lys
480 485 490
atg cta att aga tat ttg caa tgt tgt tat tat gag aaa ata aa atg 1536
Met Leu Ile Arg Tyr Leu Gln Cys Cys Tyr Tyr Glu Lys Ile Met
495 500 505
aaa ata cta ttt gtc att aca ggt tta ggc ctt gga ggt gct gag aag 1584
Lys Ile Leu Phe Val Ile Thr Gly Leu Gly Leu Gly Gly Ala Glu Lys
510 515 520 525
cag gtt tgt ctt tta gct gat aaa tta agt tta agc ggg cac cat gta 1632
Gln Val Cys Leu Leu Ala Asp Lys Leu Ser Leu Ser Gly His His Val
530 535 540
aag att att tca ctt gga cat atg tct aat aat aaa gtc ttt cct agc 1680
Lys Ile Ile Ser Leu Gly His Met Ser Asn Asn Lys Val Phe Pro Ser
545 550 555
gaa aat aat gtt aat gtc att aat gta aat atg tca aaa aac att tct 1728
Glu Asn Asn Val Asn Val Ile Asn Val Asn Met Ser Lys Asn Ile Ser
560 565 570
gga gtt ata aaa ggt tgt gtc aga att aga gat gtt ata gct aat ttc 1776
Gly Val Ile Lys Gly Cys Val Arg Ile Arg Asp Val Ile Ala Asn Phe
575 580 585
aaa cca gac att gta cac agt cat atg ttt cat gca aac att atc act 1824
Lys Pro Asp Ile Val His Ser His Met Phe His Ala Asn Ile Ile Thr
590 595 600 605
aga ttg tct gta att gga atc aaa aac aga cct ggt att ata tca act 1872
Arg Leu Ser Val Ile Gly Ile Lys Asn Arg Pro Gly Ile Ile Ser Thr
610 615 620
gca cat aat aaa aat gaa ggt ggg tat ttc aga atg ctc aca tat aga 1920
Ala His Asn Lys Asn Glu Gly Gly Tyr Phe Arg Met Leu Thr Tyr Arg
625 630 635
ata acc gat tgt tta agt gat tgt tgt aca aat gtt agc aaa gaa gca 1968
Ile Thr Asp Cys Leu Ser Asp Cys Cys Thr Asn Val Ser Lys Glu Ala
640 645 650
gtg gat gag ttt tta cgg ata aaa gcc ttt aat ccc gct aaa gca att 2016
Val Asp Glu Phe Leu Arg Ile Lys Ala Phe Ash Pro Ala Lys Ala Ile
655 660 665
act atg tat aat ggg ata gat acc aat aaa ttt aaa ttt gat tta ttg 2064
Thr Met Tyr Asn Gly Ile Asp Thr Asn Lys Phe Lys Phe Asp Leu Leu
670 675 680 685
gca agg agg gaa att cga gac ggt att aat ata aaa aat gat gat ata 2112
Ala Arg Arg Glu Ile Arg Asp Gly Ile Asn Ile Lys Asn Asp Asp Ile
690 695 700
tta tta ctt gct gca ggt cgt tta acg tta gct aaa gat tat cct aat 2160
Leu Leu Leu Ala Ala Gly Arg Leu Thr Leu Ala Lys Asp Tyr Pro Asn
705 710 715
tta ttg aat gca atg act ctg ctt cct gaa cac ttt aaa ctt att att 2208
Leu Leu Asn Ala Met Thr Leu Leu Pro Glu His Phe Lys Leu Ile Ile
720 725 730
att ggt gat ggt gaa ttg cgt gac gaa att aat atg ctt ata aaa aaa 2256
Ile Gly Asp Gly Glu Leu Arg Asp Glu Ile Asn Met Leu Ile Lys Lys
735 740 745
ttg caa tta tct aat agg gtg tcc ttg ttg gga gtt aaa aaa aat att 2304
Leu Gln Leu Ser Asn Arg Val Ser Leu Leu Gly Val Lys Lys Asn Ile
750 755 760 765
gct ccc tat ttt tct gca tgt gat att ttt gtt ctc tct tct cgt tgg 2352
Ala Pro Tyr Phe Ser Ala Cys Asp Ile Phe Val Leu Ser Ser Arg Trp
770 775 780
gaa gga ttt gga tta gtc gtg gca gaa gct atg tca tgt gag cga att 2400
Glu Gly Phe Gly Leu Val Val Ala Glu Ala Met Ser Cys Glu Arg Ile
785 790 795
gtt gtt ggc acg gat tca ggg gga gta aga gaa gtt att ggt gac gat 2448
Val Val Gly Thr Asp Ser Gly Gly Val Arg Glu Val Ile Gly Asp Asp
800 805 810
gat ttt ctt gta ccc ata tct gat tca aca caa ctt gca agc aaa att 2496
Asp Phe Leu Val Pro Ile Ser Asp Ser Thr Gln Leu Ala Ser Lys Ile
815 820 825
gaa aaa ttg tct ttg agc cag ata cgt gat cac att ggt ttt cgg aat 2544
Glu Lys Leu Ser Leu Ser Gln Ile Arg Asp His Ile Gly Phe Arg Asn
830 835 840 845
cgt gag cgt att tta aaa aat ttc tca ata gat act att att atg cag 2592
Arg Glu Arg Ile Leu Lys Asn Phe Ser Ile Asp Thr Ile Ile Met Gln
850 855 860
tgg caa gaa ctc tat gga act ata att tgc tca aaa cat gaa agg 2637
Trp Gln Glu Leu Tyr Gly Thr Ile Ile Cys Ser Lys His Glu Arg
865 870 875
tagatttata tttggaacgt gtcttttgtt tgaatttaat tcaatctcaa ttgagatttt 2697
tgtatttcaa aaataccatc atagctaacg atgattggta tttattttaa gatgctttct 2757
ataaatatat tgacgttttt aatgcgccga aacgattggg ctgggaacag agaagtaaaa 2817
ctgttttgag aatgaagagt ttttgagatg tttatggata ttaaaaattg atccagtgaa 2877
ttaattattt ataataaatc aagatttaat gttaataaat gataatcttt tctgacactc 2937
atattaatta tgagtggtac gtttggtaaa cggtaaacta ttat atg aca gct aga 2993
Met Thr Ala Arg
880
aca act aaa gtt ttg cac tta caa tta ctc cca ctc tta agt ggc gtt 3041
Thr Thr Lys Val Leu His Leu Gln Leu Leu Pro Leu Leu Ser Gly Val
885 890 895
caa agg gta aca tta aac gaa att agt gcg tta tat act gat tat gat 3089
Gln Arg Val Thr Leu Asn Glu Ile Ser Ala Leu Tyr Thr Asp Tyr Asp
900 905 910
tat aca cta gtt tgc tca aaa aaa ggt cca cta aca aaa gca ttg ctg 3137
Tyr Thr Leu Val Cys Ser Lys Lys Gly Pro Leu Thr Lys Ala Leu Leu
915 920 925
gaa tat gat gtc gat tgt cat tgt atc ccc gaa ctt acg aga gaa att 3185
Glu Tyr Asp Val Asp Cys His Cys Ile Pro Glu Leu Thr Arg Glu Ile
930 935 940
acc gta aag aat gat ttt aaa gca ttg ttc aag ctt tat aag ttc ata 3233
Thr Val Lys Asn Asp Phe Lys Ala Leu Phe Lys Leu Tyr Lys Phe Ile
945 950 955 960
aaa aaa gaa aaa ttt gac att gtg cat aca cat tct tca aaa aca ggt 3281
Lys Lys Glu Lys Phe Asp Ile Val His Thr His Ser Ser Lys Thr Gly
965 970 975
att ttg ggg cga gtt gct gcc aaa tta gca cgt gtt gga aag gtg atc 3329
Ile Leu Gly Arg Val Ala Ala Lys Leu Ala Arg Val Gly Lys Val Ile
980 985 990
cac act gta cat ggt ttt tct ttt cca gcc gca tct agt aaa aaa agt 3377
His Thr Val His Gly Phe Ser Phe Pro Ala Ala Ser Ser Lys Lys Ser
995 1000 1005
tat tac ctt tat ttt ttc atg gaa tgg ata gca aag ttc ttt acg gat 3425
Tyr Tyr Leu Tyr Phe Phe Met Glu Trp Ile Ala Lys Phe Phe Thr Asp
1010 1015 1020
aag tta atc gtc ttg aat gta gat gat gaa tat ata gca ata aac aaa 3473
Lys Leu Ile Val Leu Asn Val Asp Asp Glu Tyr Ile Ala Ile Asn Lys
1025 1030 1035 1040
tta aaa ttc aag cgg gat aaa gtt ttt tta att cct aat gga gta gac 3521
Leu Lys Phe Lys Arg Asp Lys Val Phe Leu Ile Pro Asn Gly Val Asp
1045 1050 1055
act gat aag ttt tct cct tta gaa aat aaa att tat agt agc acc ttg 3569
Thr Asp Lys Phe Ser Pro Leu Glu Asn Lys Ile Tyr Ser Ser Thr Leu
1060 1065 1070
aat cta gta atg gtt ggt aga tta tcc aag caa aaa gat cct gag aca 3617
Asn Leu Val Met Val Gly Arg Leu Ser Lys Gln Lys Asp Pro Glu Thr
1075 1080 1085
tta ttg ctt gct gtt gaa aaa ctg ctg aat gaa aat gtt aat gtt aag 3665
Leu Leu Leu Ala Val Glu Lys Leu Leu Asn Glu Asn Val Asn Val Lys
1090 1095 1100
ctg aca ctt gta gga gat ggt gaa cta aaa gaa cag tta gaa agc agg 3713
Leu Thr Leu Val Gly Asp Gly Glu Leu Lys Glu Gln Leu Glu Ser Arg
1105 1110 1115 1120
ttc aaa cgg caa gat gga cgt ata att ttt cat gga tgg tca gat aac 3761
Phe Lys Arg Gln Asp Gly Arg Ile Ile Phe His Gly Trp Ser Asp Asn
1125 1130 1135
att gtt aat att tta aaa gtt aat gat ctt ttt ata tta cct tct ctt 3809
Ile Val Asn Ile Leu Lys Val Asn Asp Leu Phe Ile Leu Pro Ser Leu
1140 1145 1150
tgg gag ggt atg cca tta gca att tta gaa gca ttg agc tgt gga ctt 3857
Trp Glu Gly Met Pro Leu Ala Ile Leu Glu Ala Leu Ser Cys Gly Leu
1155 1160 1165
cca tgt ata gtc act aat att cca ggt aat aat agc tta ata gaa gat 3905
Pro Cys Ile Val Thr Asn Ile Pro Gly Asn Asn Ser Leu Ile Glu Asp
1170 1175 1180
ggc tat aat ggt tgt ttg ttt gaa att aga gat tgt cag tta tta tct 3953
Gly Tyr Asn Gly Cys Leu Phe Glu Ile Arg Asp Cys Gln Leu Leu Ser
1185 1190 1195 1200
caa aaa atc atg tca tat gtt ggt aag cca gaa ctg att gca cag caa 4001
Gln Lys Ile Met Ser Tyr Val Gly Lys Pro Glu Leu Ile Ala Gln Gln
1205 1210 1215
tct acc aat gca cga tca ttt att ctg aaa aat tat gga tta gtt aaa 4049
Ser Thr Asn Ala Arg Ser Phe Ile Leu Lys Asn Tyr Gly Leu Val Lys
1220 1225 1230
aga aat aat aag gtc aga cag cta tat gat aat taaatgaaac cgaaaagtta 4102
Arg Asn Asn Lys Val Arg Gln Leu Tyr Asp Asn
1235 1240
aaaaagaaca ggtttttcaa agtgaaaata aaattacagt ttttttattg caatgattaa 4162
cgtaacatct gcattacatt caagccgcac aaccccgcgg tgaccacccc tgacaggagt 4222
aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatggga cgcaacctcg 4282
cgctcaacat cgaaagccgt ggttataccg tctctatttt caaccgttcc cgtgaaaaga 4342
cggaagaagt tattgccgaa aatccaggca agaaactggt tccttactat acggtgaaag 4402
agttcgttga atctcttgaa acgcctcgtc gcatcctgtt aatgggttaa agcaggtgca 4462
ggcacggatg ctgctattga ttccctgaaa ccatatctcg ataaaggcga tatcatcatt 4522
gatgggtggg taataccttc tttcaggaca ccattcgtcg taaccgcgag ctttctgcac 4582
aaggctttac ttcatcggta cc 4604
<210>23
<211>247
<212>PRT
<213>大肠杆菌
<400>23
Val Asp Asp Asn Leu Lys Gly Gln Gly Ala Gly Lys Asn Phe Leu Ser
1 5 10 15
Leu Ile Lys Tyr Ser Glu Thr Asp Tyr Thr Ile Tyr Cys Asp Gln Asp
20 25 30
Asp Ile Trp Leu Glu Asn Lys Ile Phe Glu Leu Val Lys Tyr Ala Asn
35 40 45
Glu Ile Lys Leu Asn Val Ser Asp Ala Pro Ser Leu Val Tyr Ala Asp
50 55 60
Gly Tyr Ala Tyr Met Asp Gly Glu Gly Thr Ile Asp Phe Ser Gly Ile
65 70 75 80
Ser Asn Asn His Ala Asp Gln Leu Lys Asp Phe Leu Phe Phe Asn Gly
85 90 95
Gly Tyr Gln Gly Cys Ser Ile Met Phe Asn Arg Ala Met Thr Lys Phe
100 105 110
Leu Leu Asn Tyr Arg Gly Phe Val Tyr Leu His Asp Asp Ile Thr Thr
115 120 125
Leu Ala Ala Tyr Ala Leu Gly Lys Val Tyr Phe Leu Pro Lys Tyr Leu
130 135 140
Met Leu Tyr Arg Gln His Thr Asn Ala Val Thr Gly Ile Lys Thr Phe
145 150 155 160
Arg Asn Gly Leu Thr Ser Lys Phe Lys Ser Pro Val Asn Tyr Leu Leu
165 170 175
Ser Arg Lys His Tyr Gln Val Lys Lys Ser Phe Phe Glu Cys Asn Ser
180 185 190
Ser Ile Leu Ser Glu Thr Asn Lys Lys Val Phe Leu Asp Phe Ile Ser
195 200 205
Phe Cys Glu Ser Asn Asn Lys Phe Thr Asp Phe Phe Lys Leu Trp Arg
210 215 220
Gly Gly Phe Arg Leu Asn Asn Ser Arg Thr Lys Leu Leu Leu Lys Phe
225 230 235 240
Leu Ile Arg Arg Lys Phe Ser
245
<210>24
<211>261
<212>PRT
<213>大肠杆菌
<400>24
Met Ile Ser Ile Leu Thr Pro Thr Phe Asn Arg Gln His Thr Leu Ser
1 5 10 15
Arg Leu Phe Asn Ser Leu Ile Leu Gln Thr Asp Lys Asp Phe Glu Trp
20 25 30
Ile Ile Ile Asp Asp Gly Ser Ile Asp Ala Thr Ala Val Leu Val Glu
35 40 45
Asp Phe Arg Lys Lys Cys Asp Phe Asp Leu Ile Tyr Cys Tyr Gln Glu
50 55 60
Asn Asn Gly Lys Pro Met Ala Leu Asn Ala Gly Val Lys Ala Cys Arg
65 70 75 80
Gly Asp Tyr Ile Phe Ile Val Asp Ser Asp Asp Ala Leu Thr Pro Asp
85 90 95
Ala Ile Lys Leu Ile Lys Glu Ser Ile His Asp Cys Leu Ser Glu Lys
100 105 110
Glu Ser Phe Ser Gly Val Gly Phe Arg Lys Ala Tyr Ile Lys Gly Gly
115 120 125
Ile Ile Gly Asn Asp Leu Asn Asn Ser Ser Glu His Ile Tyr Tyr Leu
130 135 140
Asn Ala Thr Glu Ile Ser Asn Leu Ile Asn Gly Asp Val Ala Tyr Cys
145 150 155 160
Phe Lys Lys Glu Ser Leu Val Lys Asn Pro Phe Pro Arg Ile Glu Asp
165 170 175
Glu Lys Phe Val Pro Glu Leu Tyr Ile Trp Asn Lys Ile Thr Asp Lys
180 185 190
Ala Lys Ile Arg Phe Asn Ile Ser Lys Val Ile Tyr Leu Cys Glu Tyr
195 200 205
Leu Asp Asp Gly Leu Ser Lys Asn Phe His Asn Gln Leu Lys Lys Tyr
210 215 220
Pro Lys Gly Phe Lys Ile Tyr Tyr Lys Asp Gln Arg Lys Arg Glu Lys
225 230 235 240
Thr Tyr Ile Lys Lys Thr Lys Met Leu Ile Arg Tyr Leu Gln Cys Cys
245 250 255
Tyr Tyr Glu Lys Ile
260
<210>25
<211>368
<212>PRT
<213>大肠杆菌
<400>25
Met Lys Ile Leu Phe Val Ile Thr Gly Leu Gly Leu Gly Gly Ala Glu
1 5 10 15
Lys Gln Val Cys Leu Leu Ala Asp Lys Leu Ser Leu Ser Gly His His
20 25 30
Val Lys Ile Ile Ser Leu Gly His Met Ser Asn Asn Lys Val Phe Pro
35 40 45
Ser Glu Asn Asn Val Asn Val Ile Asn Val Asn Met Ser Lys Asn Ile
50 55 60
Ser Gly Val Ile Lys Gly Cys Val Arg Ile Arg Asp Val Ile Ala Asn
65 70 75 80
Phe Lys Pro Asp Ile Val His Ser His Met Phe His Ala Asn Ile Ile
85 90 95
Thr Arg Leu Ser Val Ile Gly Ile Lys Asn Arg Pro Gly Ile Ile Ser
100 105 110
Thr Ala His Asn Lys Asn Glu Gly Gly Tyr Phe Arg Met Leu Thr Tyr
115 120 125
Arg Ile Thr Asp Cys Leu Ser Asp Cys Cys Thr Asn Val Ser Lys Glu
130 135 140
Ala Val Asp Glu Phe Leu Arg Ile Lys Ala Phe Asn Pro Ala Lys Ala
145 150 155 160
Ile Thr Met Tyr Asn Gly Ile Asp Thr Asn Lys Phe Lys Phe Asp Leu
165 170 175
Leu Ala Arg Arg Glu Ile Arg Asp Gly Ile Asn Ile Lys Asn Asp Asp
180 185 190
Ile Leu Leu Leu Ala Ala Gly Arg Leu Thr Leu Ala Lys Asp Tyr Pro
195 200 205
Asn Leu Leu Asn Ala Met Thr Leu Leu Pro Glu His Phe Lys Leu Ile
210 215 220
Ile Ile Gly Asp Gly Glu Leu Arg Asp Glu Ile Asn Met Leu Ile Lys
225 230 235 240
Lys Leu Gln Leu Ser Asn Arg Val Ser Leu Leu Gly Val Lys Lys Asn
245 250 255
Ile Ala Pro Tyr Phe Ser Ala Cys Asp Ile Phe Val Leu Ser Ser Arg
260 265 270
Trp Glu Gly Phe Cly Leu Val Val Ala Glu Ala Met Ser Cys Glu Arg
275 280 285
Ile Val Val Gly Thr Asp Ser Gly Gly Val Arg Glu Val Ile Gly Asp
290 295 300
Asp Asp Phe Leu Val Pro Ile Ser Asp Ser Thr Gln Leu Ala Ser Lys
305 310 315 320
Ile Glu Lys Leu Ser Leu Ser Gln Ile Arg Asp His Ile Gly Phe Arg
325 330 335
Asn Arg Glu Arg Ile Leu Lys Asn Phe Ser Ile Asp Thr Ile Ile Met
340 345 350
Gln Trp Gln Glu Leu Tyr Gly Thr Ile Ile Cys Ser Lys His Glu Arg
355 360 365
<210>26
<211>367
<212>PRT
<213>大肠杆菌
<400>26
Met Thr Ala Arg Thr Thr Lys Val Leu His Leu Gln Leu Leu Pro Leu
1 5 10 15
Leu Ser Gly Val Gln Arg Val Thr Leu Asn Glu Ile Ser Ala Leu Tyr
20 25 30
Thr Asp Tyr Asp Tyr Thr Leu Val Cys Ser Lys Lys Gly Pro Leu Thr
35 40 45
Lys Ala Leu Leu Glu Tyr Asp Val Asp Cys His Cys Ile Pro Glu Leu
50 55 60
Thr Arg Glu Ile Thr Val Lys Asn Asp Phe Lys Ala Leu Phe Lys Leu
65 70 75 80
Tyr Lys Phe Ile Lys Lys Glu Lys Phe Asp Ile Val His Thr His Ser
85 90 95
Ser Lys Thr Gly Ile Leu Gly Arg Val Ala Ala Lys Leu Ala Arg Val
100 105 110
Gly Lys Val Ile His Thr Val His Gly Phe Ser Phe Pro Ala Ala Ser
115 120 125
Ser Lys Lys Ser Tyr Tyr Leu Tyr Phe Phe Met Glu Trp Ile Ala Lys
130 135 140
Phe Phe Thr Asp Lys Leu Ile Val Leu Asn Val Asp Asp Glu Tyr Ile
145 150 155 160
Ala Ile Asn Lys Leu Lys Phe Lys Arg Asp Lys Val Phe Leu Ile Pro
165 170 175
Asn Gly Val Asp Thr Asp Lys Phe Ser Pro Leu Glu Asn Lys Ile Tyr
180 185 190
Ser Ser Thr Leu Asn Leu Val Met Val Gly Arg Leu Ser Lys Gln Lys
195 200 205
Asp Pro Glu Thr Leu Leu Leu Ala Val Glu Lys Leu Leu Asn Glu Asn
210 215 220
Val Asn Val Lys Leu Thr Leu Val Gly Asp Gly Glu Leu Lys Glu Gln
225 230 235 240
Leu Glu Ser Arg Phe Lys Arg Gln Asp Gly Arg Ile Ile Phe His Gly
245 250 255
Trp Ser Asp Asn Ile Val Asn Ile Leu Lys Val Asn Asp Leu Phe Ile
260 265 270
Leu Pro Ser Leu Trp Glu Gly Met Pro Leu Ala Ile Leu Glu Ala Leu
275 280 285
Ser Cys Gly Leu Pro Cys Ile Val Thr Asn Ile Pro Gly Asn Asn Ser
290 295 300
Leu Ile Glu Asp Gly Tyr Asn Gly Cys Leu Phe Glu Ile Arg Asp Cys
305 310 315 320
Gln Leu Leu Ser Gln Lys Ile Met Ser Tyr Val Gly Lys Pro Glu Leu
325 330 335
Ile Ala Gln Gln Ser Thr Asn Ala Arg Ser Phe Ile Leu Lys Asn Tyr
340 345 350
Gly Leu Val Lys Arg Asn Asn Lys Val Arg Gln Leu Tyr Asp Asn
355 360 365
<210>27
<211>1272
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(319)..(1269)
<220>
<221>CDS
<222>(3)..(215)
<400>27
cc ggg aag cac tcg gcg ctg att gtt gca cat cgt ctg acc acc gcg 47
Gly Lys His Ser Ala Leu Ile Val Ala His Arg Leu Thr Thr Ala
1 5 10 15
caa cgc tgc gat ctg att gcc gtt att gat aag ggg tta ctt gcg gaa 95
Gln Arg Cys Asp Leu Ile Ala Val Ile Asp Lys Gly Leu Leu Ala Glu
20 25 30
tac gga acc cac gaa cag ctg tta tct gcg ggc ggc ctc tat acc cgc 143
Tyr Gly Thr His Glu Gln Leu Leu Ser Ala Gly Gly Leu Tyr Thr Arg
35 40 45
tta tgg cat gac agc gtc agc agt act gct ctc cat cgc cag cac aac 191
Leu Trp His Asp Ser Val Ser Ser Thr Ala Leu His Arg Gln His Asn
50 55 60
atg aag gag gaa acc ccg gga tag ttactggaca cgtaatgtat taaaaacaca 245
Met Lys Glu Glu Thr Pro Gly
65 70
gtcagaagcg gcggtaccgt gaatagccgc tttaattatt tatactgaca tccttaattt 305
ttaaagagta tga atg ctg aac atg caa caa cat ctc tct gct atc gcc 354
Met Leu Asn Met Gln Gln His Leu Ser Ala Ile Ala
75 80
agc ctg cgc aac caa ctg gca gcg ggc cac att gct aac ctt act gac 402
Ser Leu Arg Asn Gln Leu Ala Ala Gly His Ile Ala Asn Leu Thr Asp
85 90 95
ttc tgg cgc gaa gct gag tcg ctg aat gtt cct ctt gtg acg cca gtc 450
Phe Trp Arg Glu Ala Glu Ser Leu Asn Val Pro Leu Val Thr Pro Val
100 105 110 115
gaa gga gcg gaa gat gag cga gaa gtg acc ttt ctg tgg cgc gcc cga 498
Glu Gly Ala Glu Asp Glu Arg Glu Val Thr Phe Leu Trp Arg Ala Arg
120 125 130
cat cct ctg cag ggc gtt tat ctg cgt ctg aac cgg gtg acg gat aaa 546
His Pro Leu Gln Gly Val Tyr Leu Arg Leu Asn Arg Val Thr Asp Lys
135 140 145
gag cac gta gaa aaa gga atg atg agc gcc ctt ccc gaa acg gat atc 594
Glu His Val Glu Lys Gly Met Met Ser Ala Leu Pro Glu Thr Asp Ile
150 155 160
tgg aca ctg aca ctg cgt tta ccc gca agt tac tgc ggc tcc tat tcg 642
Trp Thr Leu Thr Leu Arg Leu Pro Ala Ser Tyr Cys Gly Ser Tyr Ser
165 170 175
ctg ctg gaa atc ccc ccc ggc act acg gct gag acg att gca ctg tcc 690
Leu Leu Glu Ile Pro Pro Gly Thr Thr Ala Glu Thr Ile Ala Leu Ser
180 185 190 195
gga ggc cgt ttt gcc acc ctt gcc gga aag gcc gat ccg cta aac aaa 738
Gly Gly Arg Phe Ala Thr Leu Ala Gly Lys Ala Asp Pro Leu Asn Lys
200 205 210
atg ccg gag atc aac gtt cgg gga aac gca aag gaa tca gtg ctg aca 786
Met Pro Glu Ile Asn Val Arg Gly Asn Ala Lys Glu Ser Val Leu Thr
215 220 225
ctt gat aaa gct ccc gcc ctg tcg gaa tgg aac ggc ggc ttc cac acc 834
Leu Asp Lys Ala Pro Ala Leu Ser Glu Trp Asn Gly Gly Phe His Thr
230 235 240
gga caa ctg ctt acc tcc atg cgc att atc gcc ggg aaa tct cgc cag 882
Gly Gln Leu Leu Thr Ser Met Arg Ile Ile Ala Gly Lys Ser Arg Gln
245 250 255
gtt cgg ctc tat att ccg gat gtt gat att tct cag ccc ctc ggg ctg 930
Val Arg Leu Tyr Ile Pro Asp Val Asp Ile Ser Gln Pro Leu Gly Leu
260 265 270 275
gtc gtg ctg ccc gat ggt gaa acc tgg ttt gat cac ctt ggc gta tgc 978
Val Val Leu Pro Asp Gly Glu Thr Trp Phe Asp His Leu Gly Val Cys
280 285 290
gcg gca att gac gcc gcc ata aat aat ggg cgc atc gtg ccc gtg gct 1026
Ala Ala Ile Asp Ala Ala Ile Asn Asn Gly Arg Ile Val Pro Val Ala
295 300 305
gta ctg ggc att gac aac att aat gaa cat gaa cgc act gag ata ctc 1074
Val Leu Gly Ile Asp Asn Ile Asn Glu His Glu Arg Thr Glu Ile Leu
310 315 320
ggc ggg cgc agc aaa ctg ata aag gat atc gcc gga cat ctg ctg ccg 1122
Gly Gly Arg Ser Lys Leu Ile Lys Asp Ile Ala Gly His Leu Leu Pro
325 330 335
atg att cgc gct gaa caa ccg cag cgt cag tgg gca gac cgt tcg cgc 1170
Met Ile Arg Ala Glu Gln Pro Gln Arg Gln Trp Ala Asp Arg Ser Arg
340 345 350 355
aca gtg ctg gcc ggg cag agc ctc ggc ggg atc agt gcg cta atg ggg 1218
Thr Val Leu Ala Gly Gln Ser Leu Gly Gly Ile Ser Ala Leu Met Gly
360 365 370
gct cgt tac gca ccg gaa acg ttc ggt ctg gtg ctc agc cac tct cct 1266
Ala Arg Tyr Ala Pro Glu Thr Phe Gly Leu Val Leu Ser His Ser Pro
375 380 385
caa tgc 1272
Gln
<210>28
<211>70
<212>PRT
<213>大肠杆菌
<400>28
Gly Lys His Ser Ala Leu Ile Val Ala His Arg Leu Thr Thr Ala Gln
1 5 10 15
Arg Cys Asp Leu Ile Ala Val Ile Asp Lys Gly Leu Leu Ala Glu Tyr
20 25 30
Gly Thr His Glu Gln Leu Leu Ser Ala Gly Gly Leu Tyr Thr Arg Leu
35 40 45
Trp His Asp Ser Val Ser Ser Thr Ala Leu His Arg Gln His Asn Met
50 55 60
Lys Glu Glu Thr Pro Gly
65 70
<210>29
<211>317
<212>PRT
<213>大肠杆菌
<400>29
Met Leu Asn Met Gln Gln His Leu Ser Ala Ile Ala Ser Leu Arg Asn
1 5 10 15
Gln Leu Ala Ala Gly His Ile Ala Asn Leu Thr Asp Phe Trp Arg Glu
20 25 30
Ala Glu Ser Leu Asn Val Pro Leu Val Thr Pro Val Glu Gly Ala Glu
35 40 45
Asp Glu Arg Glu Val Thr Phe Leu Trp Arg Ala Arg His Pro Leu Gln
50 55 60
Gly Val Tyr Leu Arg Leu Asn Arg Val Thr Asp Lys Glu His Val Glu
65 70 75 80
Lys Gly Met Met Ser Ala Leu Pro Glu Thr Asp Ile Trp Thr Leu Thr
85 90 95
Leu Arg Leu Pro Ala Ser Tyr Cys Gly Ser Tyr Ser Leu Leu Glu Ile
100 105 110
Pro Pro Gly Thr Thr Ala Glu Thr Ile Ala Leu Ser Gly Gly Arg Phe
115 120 125
Ala Thr Leu Ala Gly Lys Ala Asp Pro Leu Asn Lys Met Pro Glu Ile
130 135 140
Asn Val Arg Gly Asn Ala Lys Glu Ser Val Leu Thr Leu Asp Lys Ala
145 150 155 160
Pro Ala Leu Ser Glu Trp Asn Gly Gly Phe His Thr Gly Gln Leu Leu
165 170 175
Thr Ser Met Arg Ile Ile Ala Gly Lys Ser Arg Gln Val Arg Leu Tyr
180 185 190
Ile Pro Asp Val Asp Ile Ser Gln Pro Leu Gly Leu Val Val Leu Pro
195 200 205
Asp Gly Glu Thr Trp Phe Asp His Leu Gly Val Cys Ala Ala Ile Asp
210 215 220
Ala Ala Ile Asn Asn Gly Arg Ile Val Pro Val Ala Val Leu Gly Ile
225 230 235 240
Asp Asn Ile Asn Glu His Glu Arg Thr Glu Ile Leu Gly Gly Arg Ser
245 250 255
Lys Leu Ile Lys Asp Ile Ala Gly His Leu Leu Pro Met Ile Arg Ala
260 265 270
Glu Gln Pro Gln Arg Gln Trp Ala Asp Arg Ser Arg Thr Val Leu Ala
275 280 285
Gly Gln Ser Leu Gly Gly Ile Ser Ala Leu Met Gly Ala Arg Tyr Ala
290 295 300
Pro Glu Thr Phe Gly Leu Val Leu Ser His Ser Pro Gln
305 310 315
<210>30
<211>4039
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(1)..(285)
<220>
<221>CDS
<222>(370). (1326)
<400>30
cct tca atg tgg tgg acg cca gaa aga acc agt cga cca ggc ttg ttc 48
Pro Ser Met Trp Trp Thr Pro Glu Arg Thr Ser Arg Pro Gly Leu Phe
1 5 10 15
agc gaa acc gat acc tca tgg gtg agt gag cat ctg ctt tct gcc cca 96
Ser Glu Thr Asp Thr Ser Trp Val Ser Glu His Leu Leu Ser Ala Pro
20 25 30
ccg cag ggc gta cgt atc agc ctg tgc gtg gga tcg ctg gaa ggt tcg 144
Pro Gln Gly Val Arg Ile Ser Leu Cys Val Gly Ser Leu Glu Gly Ser
35 40 45
aca gtg cct cac gtt cag cag ctt cac cag cgg ctg att acc gct ggc 192
Thr Val Pro His Val Gln Gln Leu His Gln Arg Leu Ile Thr Ala Gly
50 55 60
gtc gaa agc cat tgc gca atc tac acc ggt ggt cac gat tac gca tgg 240
Val Glu Ser His Cys Ala Ile Tyr Thr Gly Gly His Asp Tyr Ala Trp
65 70 75 80
tgg cgc ggt gca ctg att gac ggg att ggt tta cta cag ggt tga 285
Trp Arg Gly Ala Leu Ile Asp Gly Ile Gly Leu Leu Gln Gly
85 90 95
gttgacccac aaacactttc aggaaacggt acagacttcc tgaataaatc aaatagtcac 345
ctgcggaaaa ggaataatca tcag atg tat gcc cgc gag tat cgc tca aca 396
Met Tyr Ala Arg Glu Tyr Arg Ser Thr
100
cgc ccg cat aaa gcg att ttc ttt cat ctt tct tgc ctc acc ctt atc 444
Arg Pro His Lys Ala Ile Phe Phe His Leu Ser Cys Leu Thr Leu Ile
105 110 115 120
tgt agt gcg caa gtt tat gcg aag ccg gat atg cgg cca ctg ggg ccg 492
Cys Ser Ala Gln Val Tyr Ala Lys Pro Asp Met Arg Pro Leu Gly Pro
125 130 135
aat ata gcc gat aaa ggc tcc gtg ttt tac cat ttc agc gtc acc tct 540
Asn Ile Ala Asp Lys Gly Ser Val Phe Tyr His Phe Ser Val Thr Ser
140 145 150
ttc gac tct gtc gat ggc aca cgc cat tat cgg gta tgg acg gcc gtg 588
Phe Asp Ser Val Asp Gly Thr Arg His Tyr Arg Val Trp Thr Ala Val
155 160 165
ccg aat aca acc gca ccg gca tcg ggt tac ccg att tta tat atg ctt 636
Pro Asn Thr Thr Ala Pro Ala Ser Gly Tyr Pro Ile Leu Tyr Met Leu
170 175 180
gac ggt aac gca gtt atg gat cgc ctg gat gac gaa ctg ctc aaa caa 684
Asp Gly Asn Ala Val Met Asp Arg Leu Asp Asp Glu Leu Leu Lys Gln
185 190 195 200
ttg tca gaa aaa aca ccg cca gtg atc gtg gct gtc ggg tat cag acc 732
Leu Ser Glu Lys Thr Pro Pro Val Ile Val Ala Val Gly Tyr Gln Thr
205 210 215
aac ctc cct ttc gat ctc aac agc agg gct tac gac tat acg cca gca 780
Asn Leu Pro Phe Asp Leu Asn Ser Arg Ala Tyr Asp Tyr Thr Pro Ala
220 225 230
gca gaa agc aga aaa aca gat ctc cac tca ggg cgt ttt agc cgt aag 828
Ala Glu Ser Arg Lys Thr Asp Leu His Ser Gly Arg Phe Ser Arg Lys
235 240 245
agt ggt ggc agc aac aac ttc cgc cag tta ctg gaa acg cgt att gcc 876
Ser Gly Gly Ser Asn Asn Phe Arg Gln Leu Leu Glu Thr Arg Ile Ala
250 255 260
cca aaa gtg gaa cag gga ctg aat atc gat cgg caa cgc cgc ggc tta 924
Pro Lys Val Glu Gln Gly Leu Asn Ile Asp Arg Gln Arg Arg Gly Leu
265 270 275 280
tgg ggg cac tcc tac ggc ggc ctc ttc gtg ctg gat tcc tgg ctg tcc 972
Trp Gly His Ser Tyr Gly Gly Leu Phe Val Leu Asp Ser Trp Leu Ser
285 290 295
tcc tct tac ttc cgg tcg tac tac agc gcc agc ccg tcg ttg ggc aga 1020
Ser Ser Tyr Phe Arg Ser Tyr Tyr Ser Ala Ser Pro Ser Leu Gly Arg
300 305 310
ggt tat gat gct ttg cta agc cgc gtt acg gcg gtt gag cct ctg caa 1068
Gly Tyr Asp Ala Leu Leu Ser Arg Val Thr Ala Val Glu Pro Leu Gln
315 320 325
ttc tgc gcc aaa cac ctg gcg ata atg gaa ggc tcg gcg aca cag ggt 1116
Phe Cys Ala Lys His Leu Ala Ile Met Glu Gly Ser Ala Thr Gln Gly
330 335 340
gat aac cgg gaa acg cat gct gtc ggg gtg ctg tcg aaa att cat acc 1164
Asp Asn Arg Glu Thr His Ala Val Gly Val Leu Ser Lys Ile His Thr
345 350 355 360
acc ctc act ata ctg aaa gat aaa ggc gtc aat gcc gta ttt tgg gat 1212
Thr Leu Thr Ile Leu Lys Asp Lys Gly Val Asn Ala Val Phe Trp Asp
365 370 375
ttc ccc aac ctg gga cac ggg ccg atg ttc aat gcc tcc ttt cgc cag 1260
Phe Pro Asn Leu Gly His Gly Pro Met Phe Asn Ala Ser Phe Arg Gln
380 385 390
gca ctg tta gat atc agt ggt gaa aac gca aat tac aca gca ggt tgt 1308
Ala Leu Leu Asp Ile Ser Gly Glu Asn Ala Asn Tyr Thr Ala Gly Cys
395 400 405
cat gag tta agc cac taa acactgcccg cttttacgcg ggcagtacgc 1356
His Glu Leu Ser His
410
ctgaaacact acgatcagaa tgatgcggta actccggcat agtaagcccg gcctggctcg 1416
ttataggtat tcgccccttc agaagatcgg aagatctgtt tattgaggat attactgacg 1476
ccgacattaa gacgcagatt tttattaata tcgtaattga agttcgtccc caccagtgaa 1536
taagcgccca gctctttacc tgacagaccg ccagtatctt cactgcgggt ttccgcatga 1596
gtacgcggtt tttgtctgcc atataacgtc cagttgacgc tggcagaaaa cgcctgggtg 1656
atggtccagt taagcgagtt attgatagta tatttcggga tgaccgacag aggattaccg 1716
gtgtcttttt gctccgaagt gatcatccat gtggcattgg tattccagtt cagacgatct 1776
ttcaccagtg ggaaagacat actggcttcg ataccgtcca ccagagcttt cccgccattc 1836
tgccacttga ggatatatgc gcctgaagcg gtttgcccga taacgttatc cccggccacg 1896
atcttattct ggtaatcatt gcggaagtag gtcacacttg cgtggtaatc ttcccaggtg 1956
aactccagcc caatttcttt attgacgctg atttccggat cgagatcttt attaccgatc 2016
aggtagcacc cgcctgatgt aatatctttt ggacagccat tgcctttcga gtagagcaga 2076
tagccttcac tggattgata caggtttggg gctttaaagg ttcgggcaac ccctgctttg 2136
actttgaaat aatcgcccaa ttcctgcgaa agattcagac tggggctgaa gttcccgccg 2196
gagtcgctga gataatcaaa gcgcaggccg ggaatgatat tcgtgccagg aaccggctca 2256
atgttatctt caatatacag cgcactgatt tgagaatgat ttttactgct gcgatccgca 2316
gcagagccag aaataccgct gatatcactg tcattcaccg tcaggctggt agaggaagga 2376
tcatcgagct tatcgcggtt ccactctgca ccaacggtca gcgtttgatc aaccatcaca 2436
ttcaaaggaa tattaagctc gccgctggtt cgccaggaac tcaggcgatt ggtcgtaaac 2496
ttttcacccg ctaaaatacg cccttcacca ccgccggata atccttcatt catgcgggta 2556
ttattggttt tctcgtaata aacaccaaag cgactttgtc cccagtccca gataccatta 2616
tgcgtaatgc cataattctg tcggtacagg cggctcgtct cttgtgccgg attttgccag 2676
gcttttcggt aactgcactg gaagaactgt tttgcgtatc gccgcataga tattcccctg 2736
gcggctatat ccggcttcga aatcgagaat ctgctgcgga tttaatttcc acgagacaac 2796
gccgttaata tctttgttac gtaccccttc atgcccggct gcgtttttcg taccgaccgg 2856
agaattaata tcccaactgt cagcatccgt tttattcaga ttaccataca aacgcgtggt 2916
aagagcatta ccagccagag gcccactaag gctgaaattg gcgcgacgcg tagcgccctc 2976
atcgctactt tccggctgat tggtgtataa cgacagcgaa ccgtgccagt cgttggtggg 3036
acgtttggta atgatgttca ccaccccccc ggctgccccc gaaccgtagc gcgccgccgc 3096
agggccgcgg atcacttcaa tacgctcaac ctgttccggt ggcacccagt tggtgtcacc 3156
gcgggtatca cgctctccac gccagctata acgcacggag ttacgtgacg tcaccggtac 3216
accatcaatt aaaattaagg tgttttccgg ccccatacca cgaatatcga tctggcggtt 3276
gttaccgcgt gtgcccgagg cgctattgcc ggtaagattg acgccaggca ttttacgaat 3336
aatatctgaa aggtcgttta ccggaggggt ctttttaata tcctcgctgg taataaccga 3396
cacgcccggc tgctgtttta atacctgctc agcggtggct tccaccacca gagtctcgtc 3456
attatcatcg tcggaggatt tggctactga tacctggcta ttcaacccaa ccaggagcac 3516
agttagcgac cagaggattt tgttaattct catacctatt ccctaataaa tgcctaactt 3576
aaaatgtttg atcgttaagc tcacatcctt gccagatatt ttttactgcc attattgttt 3636
ttatataaga atgataatta atatcattta gcaaaagaaa aagcaatccc tcacaagata 3696
aatatatcga tttttcataa atatcaaatt gatatataac atatgttttt tatttcattg 3756
tacttcagtc aaataaattt ctgaagcact gctagtagtg ccagttcagc tttctttttg 3816
actcattccg gcaaagtcag taccgttcat cttttgtact gatgttgcca ctggaaaatc 3876
ggtgcgcttg tcgatcatcg ggaattttgt cacaatttct aacggatagt gttcacattg 3936
tttctaacct gcattttcag acacgggcgc tgcttatgta tataagatca gcatcactag 3996
gtctttctgc aacactactg ctttcaacaa ggtcaggcat ttc 4039
<210>31
<211>94
<212>PRT
<213>大肠杆菌
<400>31
Pro Ser Met Trp Trp Thr Pro Glu Arg Thr Ser Arg Pro Gly Leu Phe
1 5 10 15
Ser Glu Thr Asp Thr Ser Trp Val Ser Glu His Leu Leu Ser Ala Pro
20 25 30
Pro Gln Gly Val Arg Ile Ser Leu Cys Val Gly Ser Leu Glu Gly Ser
35 40 45
Thr Val Pro His Val Gln Gln Leu His Gln Arg Leu Ile Thr Ala Gly
50 55 60
Val Glu Ser His Cys Ala Ile Tyr Thr Gly Gly His Asp Tyr Ala Trp
65 70 75 80
Trp Arg Gly Ala Leu Ile Asp Gly Ile Gly Leu Leu Gln Gly
85 90
<210>32
<211>318
<212>PRT
<213>大肠杆菌
<400>32
Met Tyr Ala Arg Glu Tyr Arg Ser Thr Arg Pro His Lys Ala Ile Phe
1 5 10 15
Phe His Leu Ser Cys Leu Thr Leu Ile Cys Ser Ala Gln Val Tyr Ala
20 25 30
Lys Pro Asp Met Arg Pro Leu Gly Pro Asn Ile Ala Asp Lys Gly Ser
35 40 45
Val Phe Tyr His Phe Ser Val Thr Ser Phe Asp Ser Val Asp Gly Thr
50 55 60
Arg His Tyr Arg Val Trp Thr Ala Val Pro Asn Thr Thr Ala Pro Ala
65 70 75 80
Ser Gly Tyr Pro Ile Leu Tyr Met Leu Asp Gly Asn Ala Val Met Asp
85 90 95
Arg Leu Asp Asp Glu Leu Leu Lys Gln Leu Ser Glu Lys Thr Pro Pro
100 105 110
Val Ile Val Ala Val Gly Tyr Gln Thr Asn Leu Pro Phe Asp Leu Asn
115 120 125
Ser Arg Ala Tyr Asp Tyr Thr Pro Ala Ala Glu Ser Arg Lys Thr Asp
130 135 140
Leu His Ser Gly Arg Phe Ser Arg Lys Ser Gly Gly Ser Asn Asn Phe
145 150 155 160
Arg Gln Leu Leu Glu Thr Arg Ile Ala Pro Lys Val Glu Gln Gly Leu
165 170 175
Asn Ile Asp Arg Gln Arg Arg Gly Leu Trp Gly His Ser Tyr Gly Gly
180 185 190
Leu Phe Val Leu Asp Ser Trp Leu Ser Ser Ser Tyr Phe Arg Ser Tyr
195 200 205
Tyr Ser Ala Ser Pro Ser Leu Gly Arg Gly Tyr Asp Ala Leu Leu Ser
210 215 220
Arg Val Thr Ala Val Glu Pro Leu Gln Phe Cys Ala Lys His Leu Ala
225 230 235 240
Ile Met Glu Gly Ser Ala Thr Gln Gly Asp Asn Arg Glu Thr His Ala
245 250 255
Val Gly Val Leu Ser Lys Ile His Thr Thr Leu Thr Ile Leu Lys Asp
260 265 270
Lys Gly Val Asn Ala Val Phe Trp Asp Phe Pro Asn Leu Gly His Gly
275 280 285
Pro Met Phe Asn Ala Ser Phe Arg Gln Ala Leu Leu Asp Ile Ser Gly
290 295 300
Glu Asn Ala Asn Tyr Thr Ala Gly Cys His Glu Leu Ser His
305 310 315
<210>33
<211>3292
<212>DNA
<213>大肠杆菌
<400>33
ccgctgcggt tgattgccgg atgcggcgtg aacgccttat ccggcctaca atcattgcaa 60
attcaataaa ttgcagcgtt ctgtaggctg gataagatgc gtcagcatcg catccggcaa 120
aggcagatct cagcgatagc gccggcttag tcagatttaa tctgcgcgcg tggtggatat 180
tttttcagga tctccatata cgcgtgcatt tcggtctgta gcggtacacc catcggaata 240
tggcgcacgc cgatagagtc gctttcctgc ggatcggtgt acaggttaaa caccgacgat 300
cccgccgttt gcattactgt gccggtgaat ccaccctgat atccgctctg ggtataagcg 360
taaggttgct gaatcaggac gtgatacttg aactcatcca tacgcacagc agaaagttta 420
ccgttgagga agtagtgctc ggccttacgg ttagactgac catttgttcc caggaagaaa 480
gatgtctggt ccacaccatc gataaaggtg gttttcggta ctaaattcgc tactttcgcc 540
ccaggatgcc ccgccagatc cagcgccgta gggaagagat ccgccagatc gacaatgccg 600
tcagatttac gcggttggat catgcctttc cagtaaacga aggttggcac acgaacgccg 660
ccttcccatg tagaaccttt cgcaccgcgg aacggcgtgc gaccgtgcgg cggaacttcg 720
gcttccggac cgttatcaga ggtgaacaca atcagcgtgt tatcaagctg accgtttttc 780
tccagtgctt tatacagatt ggcgaagata tcgttcatct ccaccatgca gtcgccgtaa 840
gaggtgcgcg ccggagagct acccgcatat ttggcgttcg ggtagttatc gaagtgacag 900
ccgcgagtgc cgtaatagag gaagaaaggc ttatcactct tcgccatctt gtcgaggaac 960
ttaacgccat attccatcca gcgttgatcc aggtcttcca tatatttcgg cgtaatgtcg 1020
gcaatggctt cctgttcacc gccgcgcacg gcatgaacgt catctttgct gaacggcagt 1080
ttttggatgt attcagaacg agccgggctc agggcgactt ccgggttgac atgaacatca 1140
cgccattcgg tatacatatc ggacaccgag ttaaagccgc ggaaatcatc aaagccaacg 1200
ttctgcggct gcgactcttt gttttccccc atatgccatt tcccgatagc ctgggtgacg 1260
tagccctgat cgtgcagcaa ctgcggcagc gtggttaacc cttgcagacc gcccggttgc 1320
ccgtacattg gcggcatcag aatgccgtgg tggatggagt attgtccggt gagaatcgtg 1380
gcgcgggttg gggaagaact cggttgagaa tacgccgaag ttaaaatcag cccctggctg 1440
gcaacggcgt cgatatctgg tgtagggtta cccaccgcca cgccgccgcc gttaaagcca 1500
acgtccatcc agcccacatc atccagcaag aaaacaacca cgttcggttt cttaccggtt 1560
tttttctcaa gttccgccag tttctgctgg gtttctttgt cttgtgcagg atgctgcatc 1620
accggcatca tgttgtcggc aatagtggtc gccggtttaa ccagatactg gtttgggtga 1680
tcgtatccgg caaagccttt gcgtgcggtg gcggttgacg gggtatctgc tgcgctggct 1740
atgagaggaa gagctgcggc gacagcaaca acaagaagtt tgggtgaaaa cgaaaattcc 1800
atgcaaaatg ctccggtttc atgtcgtcaa aatgatgacg taattaagca ttgataattg 1860
agatccctct ccctgacagg atgattgcat aaataatagt gataaaaata aattatttat 1920
ttatccagaa atgaattgaa aaatcaggag agcattttca atcctacctc tggcgcaggt 1980
gatattgtaa ggcggtgatg ttatatcgcg ttgattattg atgctgtttt tagttttaac 2040
ggcattaata tatatgttat taattgaatg acttttatta ttcattatat atatgtgtag 2100
aattgtgcgc aggagaaata ttcactcagg aagttattac tcaggaagca aagaggatta 2160
cagaattatc tcataacaag tgttaaggga tgttatttcc cggttctctg tggcataata 2220
aacgagtaga tgctcattcc atctcttatg ttcgccttag tgcctcataa actccggaat 2280
gacgcagagc cgtttacggt gcttatcgtc cactgacaga tgtcgcttat gcctcatcag 2340
acaccatgga cacaacgttg agtgaagcac ccacttgttg tcatacagac ctgttttaac 2400
gcctgctccg taataagagc aggcgttttt ttatatatca gaaaggcccc ggaggtgctt 2460
gcctccgggt gagaaagagc tactgtggcg ggttgttctg caacgttaac atcaaaccgt 2520
cgcgacgcat cgctgcggct tcttccggct tgtgcagtct gtccagcgcg tcggcaagcc 2580
atgcgtaatc gtaggcgtcc ggacgttgtt tcagcgctgc gcggaaggcg agcgatgctt 2640
cctgccattc tccgtgtttc atcagcgact gacccagtgt gctccacaac agcgggcgat 2700
cgcccacgtt tttgatctgt tggcgcagca ctttttccaa gctgttcccg ggttaattgg 2760
gttttcagac gccggggatc ggcagcagca ggccgaatcg tcatactggc gtttcaggcc 2820
atcgatgata atttgctggg cagtatcatg atcgtcacac tcaataagat gttccgccat 2880
tgccacctgc aaggccacct gatgacgcgt tttccggctt tggtttttcc accagttacg 2940
caaaccttca ctaccgttat cggcacgcgc ctgatccatc aggccaatcc atgcctgttg 3000
ttccagcatt gcacgatgtt cttcatcacc aacatgggct ttcgccattg atgggatgat 3060
atccagcagc gaactccatg caccagtgcg gatatacgcc tgttccgcca gacgtaatac 3120
ttccggatgg cgtggcgtaa cttccagcag cttatccacg ccgtgacgtg ctgcatggtt 3180
ttcattacgg gccagttgca gacgtacacg ggtgatttct accggaatgg tgtcattgcc 3240
ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt gcttcagcat cc 3292
<210>34
<211>11165
<212>DNA
<213>大肠杆菌
<220>
<221>CDS
<222>(3791)..(4834)
<220>
<221>CDS
<222>(10459)..(10776)
<220>
<221>CDS
<222>(10134)..(10427)
<220>
<221>CDS
<222>(9836)..(10081)
<220>
<221>CDS
<222>(7816)..(9480)
<220>
<221>CDS
<222>(4878)..(7802)
<220>
<221>CDS
<222>(3460)..(3702)
<220>
<221>CDS
<222>(3054)..(3407)
<220>
<221>CDS
<222>(2613)..(3041)
<220>
<221>CDS
<222>(2198)..(2530)
<220>
<221>CDS
<222>(1939)..(2196)
<220>
<22l>CDS
<222>(1573)..(1893)
<220>
<221>CDS
<222>(1102)..(1485)
<220>
<221>CDS
<222>(2)..(1099)
<400>34
c agc gat atg cag cgc ggt atc cag gct gca acg gct gca ctt cag ggc 49
Ser Asp Met Gln Arg Gly Ile Gln Ala Ala Thr Ala Ala Leu Gln Gly
1 5 10 15
ctg gtg ggc ggc aat atg gca ggc gcg ctg gca ggt gct tca gcg ccg 97
Leu Val Gly Gly Asn Met Ala Gly Ala Leu Ala Gly Ala Ser Ala Pro
20 25 30
gag ctg gcg aac atc atc ggt cat cac gcg ggt att gat gac aat aca 145
Glu Leu Ala Asn Ile Ile Gly His His Ala Gly Ile Asp Asp Asn Thr
35 40 45
gcg gca aaa gcc att gcc cat gcc att ctc ggt ggt gtg aca gca gcc 193
Ala Ala Lys Ala Ile Ala His Ala Ile Leu Gly Gly Val Thr Ala Ala
50 55 60
ctt cag ggc aac agt gcg gca gca ggc gca att ggt gcg ggt act ggt 241
Leu Gln Gly Asn Ser Ala Ala Ala Gly Ala Ile Gly Ala Gly Thr Gly
65 70 75 80
gaa gtg atc gcg tca gcc att gcg aaa agc ctc tac ccg ggc gta gat 289
Glu Val Ile Ala Ser Ala Ile Ala Lys Ser Leu Tyr Pro Gly Val Asp
85 90 95
ccg tcg aaa ctg aca gaa gat cag aag caa act gta agc acg ctg gca 337
Pro Ser Lys Leu Thr Glu Asp Gln Lys Gln Thr Val Ser Thr Leu Ala
100 105 110
acg ctg tca gcg ggt atg gcc ggc ggc att gcc agt ggc gat gtg gct 385
Thr Leu Ser Ala Gly Met Ala Gly Gly Ile Ala Ser Gly Asp Val Ala
115 120 125
ggc gcg gct gct gga gct ggt gcc ggg aag aac gtt gtt gag aat aat 433
Gly Ala Ala Ala Gly Ala Gly Ala Gly Lys Asn Val Val Glu Asn Asn
130 135 140
gcg ctg agt ctg gtt gcc aga ggc tgt gcg gtc gca gca cct tgc agg 481
Ala Leu Ser Leu Val Ala Arg Gly Cys Ala Val Ala Ala Pro Cys Arg
145 150 155 160
act aaa gtt gca gag cag ttg cta gaa atc ggg gcg aaa gcg ggc atg 529
Thr Lys Val Ala Glu Gln Leu Leu Glu Ile Gly Ala Lys Ala Gly Met
165 170 175
gcc ggg ctt gcc ggg gcg gca gtc aag gat atg gcc gac agg atg acc 577
Ala Gly Leu Ala Gly Ala Ala Val Lys Asp Met Ala Asp Arg Met Thr
180 185 190
tcc gat gaa ctg gag cat ctg att acc ctg caa atg atg ggt aat gat 625
Ser Asp Glu Leu Glu His Leu Ile Thr Leu Gln Met Met Gly Asn Asp
195 200 205
gag atc act act aag tat ctc agt tcg ttg cat gat aag tac ggt tcc 673
Glu Ile Thr Thr Lys Tyr Leu Ser Ser Leu His Asp Lys Tyr Gly Ser
210 215 220
ggg gct gcc tcg aat ccg aat atc ggt aaa gat ctg acc gat gcg gaa 721
Gly Ala Ala Ser Asn Pro Asn Ile Gly Lys Asp Leu Thr Asp Ala Glu
225 230 235 240
aaa gta gaa ctg ggc ggt tcc ggc tca gga acc ggt aca cca cca cca 769
Lys Val Glu Leu Gly Gly Ser Gly Ser Gly Thr Gly Thr Pro Pro Pro
245 250 255
tcg gaa aat gat cct aag cag caa aat gaa aaa act gta gat aag ctt 817
Ser Glu Asn Asp Pro Lys Gln Gln Asn Glu Lys Thr Val Asp Lys Leu
260 265 270
aat cag aag caa gaa agt gcg att aag aag atc gat aac act ata aaa 865
Asn Gln Lys Gln Glu Ser Ala Ile Lys Lys Ile Asp Asn Thr Ile Lys
275 280 285
aat gct ctg aaa gat cat gat att att gga act ctc aag gat atg gat 913
Asn Ala Leu Lys Asp His Asp Ile Ile Gly Thr Leu Lys Asp Met Asp
290 295 300
ggt aag cca gtt cct aaa gag aat gga gga tat tgg gat cat atg cag 961
Gly Lys Pro Val Pro Lys Glu Asn Gly Gly Tyr Trp Asp His Met Gln
305 310 315 320
gaa atg caa aat acg ctc aga gga tta aga aat cat gcg gat acg ttg 1009
Glu Met Gln Asn Thr Leu Arg Gly Leu Arg Asn His Ala Asp Thr Leu
325 330 335
aaa aac gtc aac aat cct gaa gct cag gct gcg tat ggc aga gca aca 1057
Lys Asn Val Asn Asn Pro Glu Ala Gln Ala Ala Tyr Gly Arg Ala Thr
340 345 350
gat gct att aat aaa ata gaa tca gcc ttg aaa gga tat gga at atg 1104
Asp Ala Ile Asn Lys Ile Glu Ser Ala Leu Lys Gly Tyr Gly Met
355 360 365
att acc tta cgt aaa ttg att gga aac atc aat atg aca aaa gag cct 1152
Ile Thr Leu Arg Lys Leu Ile Gly Asn Ile Asn Met Thr Lys Glu Pro
370 375 380
gag caa caa tca ccg ctt gaa ctc tgg ttc gaa cgt atc ata gat gtg 1200
Glu Gln Gln Ser Pro Leu Glu Leu Trp Phe Glu Arg Ile Ile Asp Val
385 390 395
cct ctt gaa aag tta aca gtg gaa gat ctt tgc cgc gct atc cga caa 1248
Pro Leu Glu Lys Leu Thr Val Glu Asp Leu Cys Arg Ala Ile Arg Gln
400 405 410 415
aat tta tgt att gat cag ttg atg cca aga gtg ttg gaa gtt cta act 1296
Asn Leu Cys Ile Asp Gln Leu Met Pro Arg Val Leu Glu Val Leu Thr
420 425 430
aaa gag ccg tta gcg ggt gaa tat tac gat ggt gaa cta att gca gct 1344
Lys Glu Pro Leu Ala Gly Glu Tyr Tyr Asp Gly Glu Leu Ile Ala Ala
435 440 445
tta tca acg ata aaa gga gaa gat cta aaa gat cag aaa agt acc ttt 1392
Leu Ser Thr Ile Lys Gly Glu Asp Leu Lys Asp Gln Lys Ser Thr Phe
450 455 460
acc caa ata agg caa ctt ata aac cag cta gaa ccg tca gat att aac 1440
Thr Gln Ile Arg Gln Leu Ile Asn Gln Leu Glu Pro Ser Asp Ile Asn
465 470 475
gat gat tta aga aaa gat ata tta aaa atc aat cag ata att gta 1485
Asp Asp Leu Arg Lys Asp Ile Leu Lys Ile Asn Gln Ile Ile Val
480 485 490
taactaatcc cggccactga gccgagatct tctttgtgtg ccgggcatgt tcagcagctt 1545
gggggtgaaa gtcccctgtc cagcctg atg gtg gcg aag gcg ttc gcg tac gca 1599
Met Val Ala Lys Ala Phe Ala Tyr Ala
495 500
ctt aac cag tgg ccg gca ctg acg tac tat gcg aac gat ggc tgg gtg 1647
Leu Asn Gln Trp Pro Ala Leu Thr Tyr Tyr Ala Asn Asp Gly Trp Val
505 510 515
gaa atc gac aac aac atc gct gaa aat gcc ctg cgg gcg gtc agt ctg 1695
Glu Ile Asp Asn Asn Ile Ala Glu Asn Ala Leu Arg Ala Val Ser Leu
520 525 530 535
ggt cgt aaa aac ttc ctg ttc ttc ggc tct gac cat ggt ggt gag cgg 1743
Gly Arg Lys Asn Phe Leu Phe Phe Gly Ser Asp His Gly Gly Glu Arg
540 545 550
gga gcg cta ctg tac agc ctg atc ggg acg tgc aaa ctg aat gac gtg 1791
Gly Ala Leu Leu Tyr Ser Leu Ile Gly Thr Cys Lys Leu Asn Asp Val
555 560 565
gat cca gaa agc tac ctt cgc cat gtg ctt gcc gtc ata gca gac tgg 1839
Asp Pro Glu Ser Tyr Leu Arg His Val Leu Ala Val Ile Ala Asp Trp
570 575 580
ccg gtc aac cgg gtc agc gaa ctg ctt ccg tgg cgc ata gca ctg cca 1887
Pro Val Asn Arg Val Ser Glu Leu Leu Pro Trp Arg Ile Ala Leu Pro
585 590 595
gct gaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac agaaa atg ctg 1944
Ala Glu Met Leu
600
atg tct gta cag aaa gaa aag aac gtc gca gag agt gtg gta tct gaa 1992
Met Ser Val Gln Lys Glu Lys Asn Val Ala Glu Ser Val Val Ser Glu
605 610 615
acg cat acc ggc gac agc gta tat gct tcc ctg ttt gaa aaa att aac 2040
Thr His Thr Gly Asp Ser Val Tyr Ala Ser Leu Phe Glu Lys Ile Asn
620 625 630 635
ctg aat ccg gta tct gcc ctg agt gca ctg gat aac cct ttc cgg tca 2088
Leu Asn Pro Val Ser Ala Leu Ser Ala Leu Asp Asn Pro Phe Arg Ser
640 645 650
gca gat aac gcg act ggc aga att acc tcc agc ata caa cct gcg gtg 2136
Ala Asp Asn Ala Thr Gly Arg Ile Thr Ser Ser Ile Gln Pro Ala Val
655 660 665
cag tgc gca gct gct gca gca act gag ggt tct tgt ccc cgg caa tcc 2184
Gln Cys Ala Ala Ala Ala Ala Thr Glu Gly Ser Cys Pro Arg Gln Ser
670 675 680
ccg tgt tca gga a atg gtg gat aac tgg cag aag agt gta agg agt cgt 2233
Pro Cys Ser Gly Met Val Asp Asn Trp Gln Lys Ser Val Arg Ser Arg
685 690 695
gcg ctc ccg gaa gag gcg atg acg ggc tgg aac gaa ggc atg atc cgc 2281
Ala Leu Pro Glu Glu Ala Met Thr Gly Trp Asn Glu Gly Met Ile Arg
700 705 710 715
tta cag cag ttg gct gag cgc ctg aac cgt cag gat gaa cag cgg gga 2329
Leu Gln Gln Leu Ala Glu Arg Leu Asn Arg Gln Asp Glu Gln Arg Gly
720 725 730
aaa tac atg acg gtc agt gaa ctg aaa acg gag gtg ttt ggc atc atg 2377
Lys Tyr Met Thr Val Ser Glu Leu Lys Thr Glu Val Phe Gly Ile Met
735 740 745
cag gct ttt aac cgg cat atc ccg gcg gaa gag cag tta cgt cgc tac 2425
Gln Ala Phe Asn Arg His Ile Pro Ala Glu Glu Gln Leu Arg Arg Tyr
750 755 760
ggt gaa gtc cgt aac cag aat ggc agt gaa cag cag caa aaa cag gct 2473
Gly Glu Val Arg Asn Gln Asn Gly Ser Glu Gln Gln Gln Lys Gln Ala
765 770 775
gaa atg gcg cta aat cag tta att aac cgt tat cag atg ata cgt gca 2521
Glu Met Ala Leu Asn Gln Leu Ile Asn Arg Tyr Gln Met Ile Arg Ala
780 785 790 795
ggc aaa caa tagtggtagc cataatgcag gagcaaagcc tgaatcagga 2570
Gly Lys Gln
agagttattc tgactgagtt tggttttctg gcgattcttg tg atg gtg gga tgt 2624
Met Val Gly Cys
800
gct tgg tta gct gaa cag gcc ttt tcc gac cat gcg ctt tca cca cac 2672
Ala Trp Leu Ala Glu Gln Ala Phe Ser Asp His Ala Leu Ser Pro His
805 810 815
agt gct tgg ccg tac agt gca tcg cgc gat gcc ggg ctg gcc gat acg 2720
Ser Ala Trp Pro Tyr Ser Ala Ser Arg Asp Ala Gly Leu Ala Asp Thr
820 825 830
ggc gcg ggc ggc tat ccc act tgt aaa cag cgg tgg gcc gac gac acc 2768
Gly Ala Gly Gly Tyr Pro Thr Cys Lys Gln Arg Trp Ala Asp Asp Thr
835 840 845 850
gtt ggg ctg aaa gcc cgt cta ctg caa ctt cct gcc cta gat atc tgg 2816
Val Gly Leu Lys Ala Arg Leu Leu Gln Leu Pro Ala Leu Asp Ile Trp
855 860 865
acg gcg ttt aaa aaa atc gac cag tcg cag gta gtg tat gaa gag gcc 2864
Thr Ala Phe Lys Lys Ile Asp Gln Ser Gln Val Val Tyr Glu Glu Ala
870 875 880
gtg ctg cgc tcg cgg gtc agt gaa cga aat atg cag gta tcg cag aat 2912
Val Leu Arg Ser Arg Val Ser Glu Arg Asn Met Gln Val Ser Gln Asn
885 890 895
ggg cgc gtt tat cca agc tat ggc ggt aac gtt gat ggc acc gtc gcc 2960
Gly Arg Val Tyr Pro Ser Tyr Gly Gly Asn Val Asp Gly Thr Val Ala
900 905 910
aat gcc gcc acc cgg ttg gca tcc ggc gct aga aat atc ctc ggc agc 3008
Asn Ala Ala Thr Arg Leu Ala Ser Gly Ala Arg Asn Ile Leu Gly Ser
915 920 925 930
ata gcg gca tgt acg gca ttc gac agc gtg cgt taggcactac cg atg gta 3059
Ile Ala Ala Cys Thr Ala Phe Asp Ser Val Arg Met Val
935 940
cag gcg cag ctg caa ata gcg ctg gtg atc tgt att ccg ctg ata acg 3107
Gln Ala Gln Leu Gln Ile Ala Leu Val Ile Cys Ile Pro Leu Ile Thr
945 950 955
ctc tgt tcg gcg tgg gat gtg aaa gta gtg atg acg ctg acg ttt gtg 3155
Leu Cys Ser Ala Trp Asp Val Lys Val Val Met Thr Leu Thr Phe Val
960 965 970 975
cag ttt gca cta ttt ttc ctc acc ttt tgg tgg gaa ctg gca cgg tgg 3203
Gln Phe Ala Leu Phe Phe Leu Thr Phe Trp Trp Glu Leu Ala Arg Trp
980 985 990
ctt gat agc tgg ctg ctg gat gtg ctc tac aac agc gat acc cac agt 3251
Leu Asp Ser Trp Leu Leu Asp Val Leu Tyr Asn Ser Asp Thr His Ser
995 1000 1005
agc tgg aat tta gcc ggg atc cag aat acg cag gat gac gtg att atc 3299
Ser Trp Asn Leu Ala Gly Ile Gln Asn Thr Gln Asp Asp Val Ile Ile
1010 1015 1020
aat ctg gtg atg agg ttg atg ttt ctg gtg ttg ccg aca ttc tgg ctg 3347
Asn Leu Val Met Arg Leu Met Phe Leu Val Leu Pro Thr Phe Trp Leu
1025 1030 1035
ggg gcg atg acg tgg gct gga gtg agg gtt ggc gtg gcg ctg aat gga 3395
Gly Ala Met Thr Trp Ala Gly Val Arg Val Gly Val Ala Leu Asn Gly
1040 1045 1050 1055
gcg ctg gcg gga tgattgggag gtgattcgcc aatctcactt tcctatacac 3447
Ala Leu Ala Gly
atataaaatg ta atg aaa tat ctc ttt ttt gag aat ata cat tct ata ttt 3498
Met Lys Tyr Leu Phe Phe Glu Asn Ile His Ser Ile Phe
1060 1065 1070
tta aca ttc agt ctc ttc cga aca tct gtg tcg cct gat ttc cca atg 3546
Leu Thr Phe Ser Leu Phe Arg Thr Ser Val Ser Pro Asp Phe Pro Met
1075 1080 1085
att ttt gca ttg ccc tca atc att tta ggt caa ttt acg acc aac caa 3594
Ile Phe Ala Leu Pro Ser Ile Ile Leu Gly Gln Phe Thr Thr Asn Gln
1090 1095 1100
tta act aac ttt gtg ata tgt atg ggt aac acc gtt gaa cgt cgg ctg 3642
Leu Thr Asn Phe Val Ile Cys Met Gly Asn Thr Val Glu Arg Arg Leu
1105 1110 1115 1120
ggt gtt gtt cat aat ccc ttt aaa agg tct ggg gat ggc cat gac ctc 3690
Gly Val Val His Asn Pro Phe Lys Arg Ser Gly Asp Gly His Asp Leu
1125 1130 1135
agg gcg gta gcg tgaccaaagt tcatatccat accaattatt tttatttaaa 3742
Arg Ala Val Ala
1140
atatcaactt attcgagttg ttttatttag ttcaaagaag gtatcaaa ttg ata gtt 3799
Leu Ile Val
ata gat ttt ttt tgt ggc tgt ggt gga gcc agt gaa ggg cta cgt cag 3847
Ile Asp Phe Phe Cys Gly Cys Gly Gly Ala Ser Glu Gly Leu Arg Gln
1145 1150 1155
gct ggc ttt gat atc gag ctt gga tta gat att gac caa caa gca tca 3895
Ala Gly Phe Asp Ile Glu Leu Gly Leu Asp Ile Asp Gln Gln Ala Ser
1160 1165 1170 1175
gaa aca ttt aaa gct aat ttc cct gat gca aaa ttc atc caa gat gat 3943
Glu Thr Phe Lys Ala Asn Phe Pro Asp Ala Lys Phe Ile Gln Asp Asp
1180 1185 1190
att agg aaa atc gaa cct caa gat atc tcc gac atc att gat att aaa 3991
Ile Arg Lys Ile Glu Pro Gln Asp Ile Ser Asp Ile Ile Asp Ile Lys
1195 1200 1205
gct aaa cgg cct ttg tta ctg agt gca tgt gca cca tgt caa cca ttt 4039
Ala Lys Arg Pro Leu Leu Leu Ser Ala Cys Ala Pro Cys Gln Pro Phe
1210 1215 1220
tcg caa cag aat aaa aat aaa act agt gac gac tca agg aga aat cta 4087
Ser Gln Gln Asn Lys Asn Lys Thr Ser Asp Asp Ser Arg Arg Asn Leu
1225 1230 1235
cta aat gaa act cat cgt ttt att aga gaa ctt ctt cct gaa tat att 4135
Leu Asn Glu Thr His Arg Phe Ile Arg Glu Leu Leu Pro Glu Tyr Ile
1240 1245 1250 1255
atg ctt gaa aat gtt cct gga atg caa aaa att gat gaa gaa aaa gaa 4183
Met Leu Glu Asn Val Pro Gly Met Gln Lys Ile Asp Glu Glu Lys Glu
1260 1265 1270
ggc cca ttt cag gag ttt att aag cta ctt aaa gag tta gag tat aac 4231
Gly Pro Phe Gln Glu Phe Ile Lys Leu Leu Lys Glu Leu Glu Tyr Asn
1275 1280 1285
tat ata tct ttt ata gcc aat gct gag aac tat ggg att ccc caa aga 4279
Tyr Ile Ser Phe Ile Ala Asn Ala Glu Asn Tyr Gly Ile Pro Gln Arg
1290 1295 1300
aga aaa aga ctc gtg ctc tta gct agt cga gta ggt aaa gtt acc cta 4327
Arg Lys Arg Leu Val Leu Leu Ala Ser Arg Val Gly Lys Val Thr Leu
1305 1310 1315
cca gag ata acc cat ggt aaa aat aaa atc cca ttc aaa act gta cga 4375
Pro Glu Ile Thr His Gly Lys Asn Lys Ile Pro Phe Lys Thr Val Arg
1320 1325 1330 1335
gat tat atc cag gac ttc aca aag tta tgt tca gga gaa acc gac ccc 4423
Asp Tyr Ile Gln Asp Phe Thr Lys Leu Cys Ser Gly Glu Thr Asp Pro
1340 1345 1350
aaa gat cct tta cat agg gct gga aca ctg agc cct ctt aac cta aaa 4471
Lys Asp Pro Leu His Arg Ala Gly Thr Leu Ser Pro Leu Asn Leu Lys
1355 1360 1365
aga att atg cac act cca gaa gga ggg gat aga aga aat tgg cca gaa 4519
Arg Ile Met His Thr Pro Glu Gly Gly Asp Arg Arg Asn Trp Pro Glu
1370 1375 1380
gag tta gtt aat aaa tgc cat aaa aat tat gat ggc cac aca gat act 4567
Glu Leu Val Asn Lys Cys His Lys Asn Tyr Asp Gly His Thr Asp Thr
1385 1390 1395
tat gga aga atg agt tgg gat aag cct gcg cct aca ctt acg acg aaa 4615
Tyr Gly Arg Met Ser Trp Asp Lys Pro Ala Pro Thr Leu Thr Thr Lys
1400 1405 1410 1415
tgt aat agt tac tcc aat ggt cgt ttt ggg cat cct gac ccc act caa 4663
Cys Asn Ser Tyr Ser Asn Gly Arg Phe Gly His Pro Asp Pro Thr Gln
1420 1425 1430
cat aga gca att agc ata aga gaa gca tca aga tta caa aca ttt cct 4711
His Arg Ala Ile Ser Ile Arg Glu Ala Ser Arg Leu Gln Thr Phe Pro
1435 1440 1445
tta agc tat gtt ttt aaa ggt tcg ctg aat tca atg gca aag caa atc 4759
Leu Ser Tyr Val Phe Lys Gly Ser Leu Asn Ser Met Ala Lys Gln Ile
1450 1455 1460
ggc aat gct gta cct tgc gaa ctc gct aga cta ttt ggg cta cat ctc 4807
Gly Asn Ala Val Pro Cys Glu Leu Ala Arg Leu Phe Gly Leu His Leu
1465 1470 1475
ata gaa aat tgt act aat aag gat tca tagatatatg gctaaaataa 4854
Ile Glu Asn Cys Thr Asn Lys Asp Ser
1480 1485
gaacaaaggc tcgagctttg gac atg ctt ggc aga caa caa att gca ggt ata 4907
Met Leu Gly Arg Gln Gln Ile Ala Gly Ile
1490 1495
cct act gcc ttg agt gag tta ttt aaa aat gct cat gat gcc tat gct 4955
Pro Thr Ala Leu Ser Glu Leu Phe Lys Asn Ala His Asp Ala Tyr Ala
1500 1505 1510
gat aat gtc gaa gtt gat ttt ttt agg aaa gaa aat ctt ctt atc ttg 5003
Asp Asn Val Glu Val Asp Phe Phe Arg Lys Glu Asn Leu Leu Ile Leu
1515 1520 1525 1530
aga gat gat gga tta ggt atg aca acc gat gaa ttt gaa gag agg tgg 5051
Arg Asp Asp Gly Leu Gly Met Thr Thr Asp Glu Phe Glu Glu Arg Trp
1535 1540 1545
ttg act att gga acc tcc agc aaa tta atc gac gat gat gca att aat 5099
Leu Thr Ile Gly Thr Ser Ser Lys Leu Ile Asp Asp Asp Ala Ile Asn
1550 1555 1560
aaa cca gca gtg gat agt aat aaa gcc ttt cgc cct atc atg gga gag 5147
Lys Pro Ala Val Asp Ser Asn Lys Ala Phe Arg Pro Ile Met Gly Glu
1565 1570 1575
aaa gga ata ggc cgt tta tct atc gca gca att gga cca cag gtg ctg 5195
Lys Gly Ile Gly Arg Leu Ser Ile Ala Ala Ile Gly Pro Gln Val Leu
1580 1585 1590
gtt ctt act agg gcc aaa aga gac aat gag ctt aag cca tta gtt gct 5243
Val Leu Thr Arg Ala Lys Arg Asp Asn Glu Leu Lys Pro Leu Val Ala
1595 1600 1605 1610
gca ttt gtt aat tgg agt tta ttt gct ata cca tca ctt gat ctt gat 5291
Ala Phe Val Asn Trp Ser Leu Phe Ala Ile Pro Ser Leu Asp Leu Asp
1615 1620 1625
gat ata gaa ata cca att aga act att atc aac gac gaa tgc ttc act 5339
Asp Ile Glu Ile Pro Ile Arg Thr Ile Ile Asn Asp Glu Cys Phe Thr
1630 1635 1640
aaa aaa act ctt gat gag atg att gag caa gca aga aat aat tta gac 5387
Lys Lys Thr Leu Asp Glu Met Ile Glu Gln Ala Arg Asn Asn Leu Asp
1645 1650 1655
tct tta tca cac aaa ata tca aaa tca aaa gta tca caa ata aat aca 5435
Ser Leu Ser His Lys Ile Ser Lys Ser Lys Val Ser Gln Ile Asn Thr
1660 1665 1670
caa tta tca tct ttt gaa ttt gat cct att cta tgg gaa aaa aaa tta 5483
Gln Leu Ser Ser Phe Glu Phe Asp Pro Ile Leu Trp Glu Lys Lys Leu
1675 1680 1685 1690
ggt ggg cta aga cta tct gga gat ggg cat gga act cac ttc ata ata 5531
Gly Gly Leu Arg Leu Ser Gly Asp Gly His Gly Thr His Phe Ile Ile
1695 1700 1705
atg cct acc gaa gaa ata tta ata gat gac att tcc acg agc gat agc 5579
Met Pro Thr Glu Glu Ile Leu Ile Asp Asp Ile Ser Thr Ser Asp Ser
1710 1715 1720
aat aaa aca tca gag cag tct tct cgc tta gaa aaa gct tta tta ggt 5627
Asn Lys Thr Ser Glu Gln Ser Ser Arg Leu Glu Lys Ala Leu Leu Gly
1725 1730 1735
ttt aca aac aca atg tac agt gat tca aac cct cct att ata gct cgt 5675
Phe Thr Asn Thr Met Tyr Ser Asp Ser Asn Pro Pro Ile Ile Ala Arg
1740 1745 1750
ttt aga gac tat ctg gaa gat ggt gag tgc att gac aga att agc gaa 5723
Phe Arg Asp Tyr Leu Glu Asp Gly Glu Cys Ile Asp Arg Ile Ser Glu
1755 1760 1765 1770
tca att ttt ttt aca ccg caa gaa ttc aat ctt gca gat cac cac att 5771
Ser Ile Phe Phe Thr Pro Gln Glu Phe Asn Leu Ala Asp His His Ile
1775 1780 1785
gaa gga tgg ttc aat gaa ttt ggt caa ttc agt gga act gtt tct gtt 5819
Glu Gly Trp Phe Asn Glu Phe Gly Gln Phe Ser Gly Thr Val Ser Val
1790 1795 1800
tat ggt gaa gag cca att cat cat gtc gtg act tgg aaa aat aat aat 5867
Tyr Gly Glu Glu Pro Ile His His Val Val Thr Trp Lys Asn Asn Asn
1805 1810 1815
caa tta acc caa tgc ggt cca ttt aaa ata aaa tta gcg tat att cat 5915
Gln Leu Thr Gln Cys Gly Pro Phe Lys Ile Lys Leu Ala Tyr Ile His
1820 1825 1830
ggt cgg ctt cgt gat tca cgc tta ccc atg gag ttg tgg gcc cct ctg 5963
Gly Arg Leu Arg Asp Ser Arg Leu Pro Met Glu Leu Trp Ala Pro Leu
1835 1840 1845 1850
aag gag aaa aca gat aga tat ggt ggt tta tat atc tat cga gat gga 6011
Lys Glu Lys Thr Asp Arg Tyr Gly Gly Leu Tyr Ile Tyr Arg Asp Gly
1855 1860 1865
tta aga att ttg ccc tat gga gat tca gat acg gat ttt cta aaa ata 6059
Leu Arg Ile Leu Pro Tyr Gly Asp Ser Asp Thr Asp Phe Leu Lys Ile
1870 1875 1880
gaa aag aga aga acg tta tcc gct tct gaa tat ttt ttc tca tat cga 6107
Glu Lys Arg Arg Thr Leu Ser Ala Ser Glu Tyr Phe Phe Ser Tyr Arg
1885 1890 1895
cgt ttg ttt gga gca ata gaa tta aca aaa gaa aac aat gct tca tta 6155
Arg Leu Phe Gly Ala Ile Glu Leu Thr Lys Glu Asn Asn A la Ser Leu
1900 1905 1910
gtt gaa aaa gct ggg cga gaa gga ttc att gaa aat aag cca tat aaa 6203
Val Glu Lys Ala Gly Arg Glu Gly Phe Ile Glu Asn Lys Pro Tyr Lys
1915 1920 1925 1930
cag ttt aaa gaa atg ctt gaa aat ttc ttc atc gaa atc gca aga gat 6251
Gln Phe Lys Glu Met Leu Glu Asn Phe Phe Ile Glu Ile Ala Arg Asp
1935 1940 1945
ttc ttt aag gac gat ggc gat atg tct gaa tta ttt gtt gag aca aag 6299
Phe Phe Lys Asp Asp Gly Asp Met Ser Glu Leu Phe Val Glu Thr Lys
1950 1955 1960
caa cgt aga aat gaa gaa cat gat ttg tta tct aaa aga tct aaa caa 6347
Gln Arg Arg Asn Glu Glu His Asp Leu Leu Ser Lys Arg Ser Lys Gln
1965 1970 1975
act aaa gct aaa aaa gat aga tta aag aaa gat ctg tat gat ttt ttt 6395
Thr Lys Ala Lys Lys Asp Arg Leu Lys Lys Asp Leu Tyr Asp Phe Phe
1980 1985 1990
gat aag tta gat aat gat tac tgg aat att gaa ata aat aag cta atc 6443
Asp Lys Leu Asp Asn Asp Tyr Trp Asn Ile Glu Ile Asn Lys Leu Ile
1995 2000 2005 2010
aat aaa aac gag gaa tat ttc tcc agt aca gaa ata aca gac acc aat 6491
Asn Lys Asn Glu Glu Tyr Phe Ser Ser Thr Glu Ile Thr Asp Thr Asn
2015 2020 2025
ata gat tat gta tac aat aaa att aaa gaa caa aat gat gct atc att 6539
Ile Asp Tyr Val Tyr Asn Lys Ile Lys Glu Gln Asn Asp Ala Ile Ile
2030 2035 2040
aaa aat cta cgt aat tct gtg gat ata aag aaa ccc tct gga gtt gga 6587
Lys Asn Leu Arg Asn Ser Val Asp Ile Lys Lys Pro Ser Gly Val Gly
2045 2050 2055
tta aca aaa gag tta tct aat tta tgg gat aga tat caa ata gaa aga 6635
Leu Thr Lys Glu Leu Ser Asn Leu Trp Asp Arg Tyr Gln Ile Glu Arg
2060 2065 2070
caa aaa ata ctg tta tca cta aat gag cta aaa gat aac gtt gat aga 6683
Gln Lys Ile Leu Leu Ser Leu Asn Glu Leu Lys Asp Asn Val Asp Arg
2075 2080 2085 2090
aag ctt ata gaa ctg gat aat aaa aat aat gat ttt ctc aac tta cgg 6731
Lys Leu Ile Glu Leu Asp Asn Lys Asn Asn Asp Phe Leu Asn Leu Arg
2095 2100 2105
aag aga ctt gaa gat tct ttg aat cta caa caa agt tac tat gaa aaa 6779
Lys Arg Leu Glu Asp Ser Leu Asn Leu Gln Gln Ser Tyr Tyr Glu Lys
2110 2115 2120
gaa cta aca aag tta tat aat gac gct aaa aat gct ttg aaa gat gtg 6827
Glu Leu Thr Lys Leu Tyr Asn Asp Ala Lys Asn Ala Leu Lys Asp Val
2125 2130 2135
caa tct aaa gca aat agg tta att tct gat aat aag aaa aaa cat aag 6875
Gln Ser Lys Ala Asn Arg Leu Ile Ser Asp Asn Lys Lys Lys His Lys
2140 2145 2150
agt gaa cta aaa aac att tct tat gaa ttc caa tca act aat ctc aat 6923
Ser Glu Leu Lys Asn Ile Ser Tyr Glu Phe Gln Ser Thr Asn Leu Asn
2155 2160 2165 2170
ggc aaa gat act gcg tat ata ttg gat gta aaa aga aat cta gaa agt 6971
Gly Lys Asp Thr Ala Tyr Ile Leu Asp Val Lys Arg Asn Leu Glu Ser
2175 2180 2185
aaa att gag aat act tca aac gaa gtg att aat gaa ata aga aaa cta 7019
Lys Ile Glu Asn Thr Ser Asn Glu Val Ile Asn Glu Ile Arg Lys Leu
2190 2195 2200
acc gac cag att gca ata att agt gat agt acc act tct gaa aat tta 7067
Thr Asp Gln Ile Ala Ile Ile Ser Asp Ser Thr Thr Ser Glu Asn Leu
2205 2210 2215
tca tcg gct caa gta act gaa gca atc gaa act gaa ctt gaa cat tta 7115
Ser Ser Ala Gln Val Thr Glu Ala Ile Glu Thr Glu Leu Glu His Leu
2220 2225 2230
cga gac caa caa gca aat aac gca gag tta ata cta ctt ggc atg gct 7163
Arg Asp Gln Gln Ala Asn Asn Ala Glu Leu Ile Leu Leu Gly Met Ala
2235 2240 2245 2250
ctt tct gta gta cat cat gaa ttt aat ggt aat att agg gca att aga 7211
Leu Ser Val Val His His Glu Phe Asn Gly Asn Ile Arg Ala Ile Arg
2255 2260 2265
agt gcg cta agg gaa tta aaa gca tgg gct gac aga aat cct aag ctt 7259
Ser Ala Leu Arg Glu Leu Lys Ala Trp Ala Asp Arg Asn Pro Lys Leu
2270 2275 2280
gat att ata tac caa aaa atc aga act agt ttt gat cac tta gat ggt 7307
Asp Ile Ile Tyr Gln Lys Ile Arg Thr Ser Phe Asp His Leu Asp Gly
2285 2290 2295
tat tta aaa acc ttt aca cca ttg aca aga cgt tta agt cgc tct aaa 7355
Tyr Leu Lys Thr Phe Thr Pro Leu Thr Arg Arg Leu Ser Arg Ser Lys
2300 2305 2310
acc aat ata act gga act gcc att tta gaa ttt atc aga gat gta ttc 7403
Thr Asn Ile Thr Gly Thr Ala Ile Leu Glu Phe Ile Arg Asp Val Phe
2315 2320 2325 2330
gat gat cgt ctt gag aaa gaa gga att gaa tta ttc act acc tca aag 7451
Asp Asp Arg Leu Glu Lys Glu Gly Ile Glu Leu Phe Thr Thr Ser Lys
2335 2340 2345
ttt gtt aat caa gaa att gta act tac aca tca acc att tac cct gtc 7499
Phe Val Asn Gln Glu Ile Val Thr Tyr Thr Ser Thr Ile Tyr Pro Val
2350 2355 2360
ttt ata aat cta att gat aac gca ata tac tgg ctt ggg aaa aca act 7547
Phe Ile Asn Leu Ile Asp Asn Ala Ile Tyr Trp Leu Gly Lys Thr Thr
2365 2370 2375
gga gaa aaa aga ctt ata ctt gat gct act gaa aca gga ttt gtt att 7595
Gly Glu Lys Arg Leu Ile Leu Asp Ala Thr Glu Thr Gly Phe Val Ile
2380 2385 2390
ggt gat act ggt ccc ggt gtt tca act aga gat cga gat ata ata ttt 7643
Gly Asp Thr Gly Pro Gly Val Ser Thr Arg Asp Arg Asp Ile Ile Phe
2395 2400 2405 2410
gat atg gga ttt aca cga aaa aca gga ggg cgt gga atg gga tta ttc 7691
Asp Met Gly Phe Thr Arg Lys Thr Gly Gly Arg Gly Met Gly Leu Phe
2415 2420 2425
att tcc aaa gag tgt tta tct cga gat gga ttt act ata aga ttg gat 7739
Ile Ser Lys Glu Cys Leu Ser Arg Asp Gly Phe Thr Ile Arg Leu Asp
2430 2435 2440
gat tac act cct gaa cag ggt gctttc ttt att att gag cca tca gaa 7787
Asp Tyr Thr Pro Glu Gln Gly Ala Phe Phe Ile Ile Glu Pro Ser Glu
2445 2450 2455
gaa aca agt gaa tag cggatataaa taa atg aca agc tct act gat ttt 7836
Glu Thr Ser Glu Met Thr Ser Ser Thr Asp Phe
2460 2465 2470
cat aaa ctt tct gaa gac tgc gtt cgc cgt ttt tta cat tct gta gtt 7884
His Lys Leu Ser Glu Asp Cys Val Arg Arg Phe Leu His Ser Val Val
2475 2480 2485
gct gta gat gac aat atg tct ttt gga gct ggt agt gat act ttc cct 7932
Ala Val Asp Asp Asn Met Ser Phe Gly Ala Gly Ser Asp Thr Phe Pro
2490 2495 2500
aca gac gaa gat att aat gct tta gtt gat ccc gac gat gat cct aca 7980
Thr Asp Glu Asp Ile Asn Ala Leu Val Asp Pro Asp Asp Asp Pro Thr
2505 2510 2515
cca ata ata aca gca tca gca tcc cca agg ata gaa tca act aaa tca 8028
Pro Ile Ile Thr Ala Ser Ala Ser Pro Arg Ile Glu Ser Thr Lys Ser
2520 2525 2530
aaa gca aag gta aaa aac cat cct ttt gat tac caa gct cta gca gaa 8076
Lys Ala Lys Val Lys Asn His Pro Phe Asp Tyr Gln Ala Leu Ala Glu
2535 2540 2545 2550
gct ttc gcc aaa gat ggt att gct tgt tgc gga tta tta gct aag agt 8124
Ala Phe Ala Lys Asp Gly Ile Ala Cys Cys Gly Leu Leu Ala Lys Ser
2555 2560 2565
ttt aat gtt gaa gaa aga gat ata att aca gca tca tcc cac aag gca 8172
Phe Asn Val Glu Glu Arg Asp Ile Ile Thr Ala Ser Ser His Lys Ala
2570 2575 2580
gat ata aca ata ctt gac tgg gat atg caa agc gat agt ggg caa ttt 8220
Asp Ile Thr Ile Leu Asp Trp Asp Met Gln Ser Asp Ser Gly Gln Phe
2585 2590 2595
gct att gaa ata ata aaa tcg ata atc gtt tca gat ata aat tct gga 8268
Ala Ile Glu Ile Ile Lys Ser Ile Ile Val Ser Asp Ile Asn Ser Gly
2600 2605 2610
gga cgt tta cgt ctt ctt tct att tat act ggt gaa cat gtt act gct 8316
Gly Arg Leu Arg Leu Leu Ser Ile Tyr Thr Gly Glu His Val Thr Ala
2615 2620 2625 2630
gtt ata act aag ttg aac aat gag tta aag aaa aca tac cgt agc gta 8364
Val Ile Thr Lys Leu Asn Asn Glu Leu Lys Lys Thr Tyr Arg Ser Val
2635 2640 2645
ata aaa aat gat gat agt att ttt att gaa gat aac tat gca ctc gaa 8412
Ile Lys Asn Asp Asp Ser Ile Phe Ile Glu Asp Asn Tyr Ala Leu Glu
2650 2655 2660
caa tgg tgt ata gtt gtt att agt aaa gac gtt tat gaa aaa gat ctt 8460
Gln Trp Cys Ile Val Val Ile Ser Lys Asp Val Tyr Glu Lys Asp Leu
2665 2670 2675
cca aat gtg tta ata aaa aaa ttc act aac ctt aca gct ggg ttg cta 8508
Pro Asn Val Leu Ile Lys Lys Phe Thr Asn Leu Thr Ala Gly Leu Leu
2680 2685 2690
tcc aac gcc gca ctc tct tgc att tct gaa ata aga gaa aaa acc cat 8556
Ser Asn Ala Ala Leu Ser Cys Ile Ser Glu Ile Arg Glu Lys Thr His
2695 2700 2705 2710
ggg ata tta aca aaa tat aat aat aaa tta gac act gca tat gtt tcc 8604
Gly Ile Leu Thr Lys Tyr Asn Asn Lys Leu Asp Thr Ala Tyr Val Ser
2715 2720 2725
cac atc tta aat tta ata aaa tcc aag gag tca agg gca tat gct tat 8652
His Ile Leu Asn Leu Ile Lys Ser Lys Glu Ser Arg Ala Tyr Ala Tyr
2730 2735 2740
gaa aat gct cat gat tat gca gta gat tta att tct gaa gaa ata aga 8700
Glu Asn Ala His Asp Tyr Ala Val Asp Leu Ile Ser Glu Glu Ile Arg
2745 2750 2755
tca ata ttg caa ata agt gaa aac tta aag aaa tct cta agc aaa aac 8748
Ser Ile Leu Gln Ile Ser Glu Asn Leu Lys Lys Ser Leu Ser Lys Asn
2760 2765 2770
tcc tta tcc cat tgg cct att ttt cac tat gca aaa aat ggt tgt aag 8796
Ser Leu Ser His Trp Pro Ile Phe His Tyr Ala Lys Asn Gly Cys Lys
2775 2780 2785 2790
aat ttt cta tta act gga aaa aaa caa aaa gac tta tca gta gaa cat 8844
Asn Phe Leu Leu Thr Gly Lys Lys Gln Lys Asp Leu Ser Val Glu His
2795 2800 2805
cta agg aat ata ctc tct gct gat tct tta gaa gaa att caa cac gct 8892
Leu Arg Asn Ile Leu Ser Ala Asp Ser Leu Glu Glu Ile Gln His Ala
2810 2815 2820
att gaa cac gca tct tta ggt aaa aag gaa tac tta agc caa gat ggt 8940
Ile Glu His Ala Ser Leu Gly Lys Lys Glu Tyr Leu Ser Gln Asp Gly
2825 2830 2835
gaa gaa gat aaa aag tta atg caa tta tgc tct ctg gaa atc acg cgc 8988
Glu Glu Asp Lys Lys Leu Met Gln Leu Cys Ser Leu Glu Ile Thr Arg
2840 2845 2850
agg agt tta aga tat cat tct cat ata gat aat gtg tcc tta aaa caa 9036
Arg Ser Leu Arg Tyr His Ser His Ile Asp Asn Val Ser Leu Lys Gln
2855 2860 2865 2870
gga act tta ctt tta gat gca tat aat ttt gtc tat cta tgc ata caa 9084
Gly Thr Leu Leu Leu Asp Ala Tyr Asn Phe Val Tyr Leu Cys Ile Gln
2875 2880 2885
cca tta tgt gat agc gtc aga ttg cat gaa aaa gcc gat ttt tta ttc 9132
Pro Leu Cys Asp Ser Val Arg Leu His Glu Lys Ala Asp Phe Leu Phe
2890 2895 2900
ctc agg gga aca ctg gac gat aat aat tac aat ttg tta atc gaa gat 9180
Leu Arg Gly Thr Leu Asp Asp Asn Asn Tyr Asn Leu Leu Ile Glu Asp
2905 2910 2915
gaa tat ggc ggt ttt tat aaa att aaa atg ccg gca aaa gct tct aat 9228
Glu Tyr Gly Gly Phe Tyr Lys Ile Lys Met Pro Ala Lys Ala Ser Asn
2920 2925 2930
att att tca ttt tca ttt gga gtc gaa aat gga aac ggt gtc atc ata 9276
Ile Ile Ser Phe Ser Phe Gly Val Glu Asn Gly Asn Gly Val Ile Ile
2935 2940 2945 2950
ggg aaa aag aac aat cta gtt aat act gac tat atc tca ttc gtt cct 9324
Gly Lys Lys Asn Asn Leu Val Asn Thr Asp Tyr Ile Ser Phe Val Pro
2955 2960 2965
tta ctc gtt gaa aaa ata tct act cca aaa gta ttg aaa tgg atc ggg 9372
Leu Leu Val Glu Lys Ile Ser Thr Pro Lys Val Leu Lys Trp Ile Gly
2970 2975 2980
gaa ata aaa aca acg tac gcg caa aaa ata aca act gat att gtt gct 9420
Glu Ile Lys Thr Thr Tyr Ala Gln Lys Ile Thr Thr Asp Ile Val Ala
2985 2990 2995
aat ctg tca aga ata ggt tta gat caa cat gag tgg tta cga ata aaa 9468
Asn Leu Ser Arg Ile Gly Leu Asp Gln His Glu Trp Leu Arg Ile Lys
3000 3005 3010
tca aaa gat ata taaatgatta tatatgccgt cgttttataa aaactggcgg 9520
Ser Lys Asp Ile
3015
catgtatatc tagttagtcc atcatagaag tcaagaaatt tagtttgccc tatatcttat 9580
agaaaatata ttttatatgc ttaaaaaaca ccatctttct aagatggcat ttatgtgctt 9640
tgtttcgatc aattacaact gatatattac catattgatt aattttatgt tatttaccaa 9700
agtaacggca tcttaatata tcgtcataat atagtgcgcg ttctgactct aatactgaaa 9760
aatttatttg ttctatttta cacttactgc aaatagcatc cagtttatca tatagtgtcg 9820
catcaattgg cgcag atg tca tca cgc caa atc ctt gag cat tat aat gct 9871
Met Ser Ser Arg Gln Ile Leu Glu His Tyr Asn Ala
3020 3025 3030
cta aca tat ccc cta cat caa tca atc ttg ttg cag ata atg act tcg 9919
Leu Thr Tyr Pro Leu His Gln Ser Ile Leu Leu Gln Ile Met Thr Ser
3035 3040 3045
aat ttg tta tca gtt tgc act gga aaa tcc att tac gag gat atc tcc 9967
Asn Leu Leu Ser Val Cys Thr Gly Lys Ser Ile Tyr Glu Asp Ile Ser
3050 3055 3060
ggc agt tct tgg aat atc ata cac ttc aat atc cct ctc ccc atc tct 10015
Gly Ser Ser Trp Asn Ile Ile His Phe Asn Ile Pro Leu Pro Ile Ser
3065 3070 3075
aga gcg aga ctt tcc ata ttt tct tat tgt gtc aga att aaa cct tgg 10063
Arg Ala Arg Leu Ser Ile Phe Ser Tyr Cys Val Arg Ile Lys Pro Trp
3080 3085 3090
atg agt atg gat tac atg taaccggctc atttaaaccg tctggtctgt 10111
Met Ser Met Asp Tyr Met
3095 3100
ttcctccggt tttacaaaaa ta atg tcc atc att ttt aat gga cac tat cgt 10163
Met Ser Ile Ile Phe Asn Gly His Tyr Arg
3105 3110
atg aaa cac cgg act tgg atc act gaa gct tta cgt ctt cac ttt gaa 10211
Met Lys His Arg Thr Trp Ile Thr Glu Ala Leu Arg Leu His Phe Glu
3115 3120 3125
gaa cat tta ccc cag gtt gtg gtc ggg cgt cgc ctg ggc gta cca aaa 10259
Glu His Leu Pro Gln Val Val Val Gly Arg Arg Leu Gly Val Pro Lys
3130 3135 3140
tca aca gct tgt ggt atg ttc gtg cgc ttt cgc aaa gct ggc ttt tca 10307
Ser Thr Ala Cys Gly Met Phe Val Arg Phe Arg Lys Ala Gly Phe Ser
3145 3150 3155
tgg cct ctg ccc gca ggt atg tcg gag cgg gag ctt gat ggc cgt ctt 10355
Trp Pro Leu Pro Ala Gly Met Ser Glu Arg Glu Leu Asp Gly Arg Leu
3160 3165 3170
tac ggg agt acc tcc aca gta cct gtc gta ctt tgt agt gga tcg gta 10403
Tyr Gly Ser Thr Ser Thr Val Pro Val Val Leu Cys Ser Gly Ser Val
3175 3180 3185 3190
att cag gac acc tcg aaa tcc tgt taatgttaaa acagtgaaaa tgaggtgatg 10457
Ile Gln Asp Thr Ser Lys Ser Cys
3195
c atg atc aaa act cgt cgg act aaa cgt acc ttt tcc ccg gag ttc aag 10506
Met Ile Lys Thr Arg Arg Thr Lys Arg Thr Phe Ser Pro Glu Phe Lys
3200 3205 3210
ctt gaa gct ttc gag cag gtg gtg gtt aaa tac cag cgt gat gtc aga 10554
Leu Glu Ala Phe Glu Gln Val Val Val Lys Tyr Gln Arg Asp Val Arg
3215 3220 3225 3230
gaa gtc gcg cag gca ctc gag ctc aac cct gac cat ttg cgt aaa tgg 10602
Glu Val Ala Gln Ala Leu Glu Leu Asn Pro Asp His Leu Arg Lys Trp
3235 3240 3245
ata cgg ttg tat aag cag gaa ctt cag ggt att gag cca gct ggt aat 10650
Ile Arg Leu Tyr Lys Gln Glu Leu Gln Gly Ile Glu Pro Ala Gly Asn
3250 3255 3260
gct att acc cct gaa caa cgc gaa att cag cag ctt aaa gcg cag ata 10698
Ala Ile Thr Pro Glu Gln Arg Glu Ile Gln Gln Leu Lys Ala Gln Ile
3265 3270 3275
aag cgc gtt gag atg gaa aaa gaa ata cta aag cag gct gcc gtg ctg 10746
Lys Arg Val Glu Met Glu Lys Glu Ile Leu Lys Gln Ala Ala Val Leu
3280 3285 3290
atg agc gaa atc ccc ggg aag ctg tcg cgc taatcacaca gctgaaagca 10796
Met Ser Glu Ile Pro Gly Lys Leu Ser Arg
3295 3300
aagtggccag tgtgggttat ttgtcattta ttcggtatta accgtagcgt ttattacgcg 10856
caggtgaagc gtcctgttaa tgtgcaaaga attgaattac gaagccgggt gagggctttc 10916
catgctctca gtcgtggcgc agccgggtag ccgggcaatc agtcagatgt tgcgccagag 10976
tggcgttgat gcaggccggt ggctggcatg acgactgatg cgggaatgag ggctgacaag 11036
tcgacagccg gttaaacatc acaaccgggt aaacgaagac aaaagtccgc cattgccaaa 11096
tttactgaac cggcaatttc accccgccgc accaaactgc gtctggtgcg gcgacatcag 11156
ttttattcg 11165
<210>35
<211>366
<212>PRT
<213>大肠杆菌
<400>35
Ser Asp Met Gln Arg Gly Ile Gln Ala Ala Thr Ala Ala Leu Gln Gly
1 5 10 15
Leu Val Gly Gly Asn Met Ala Gly Ala Leu Ala Gly Ala Ser Ala Pro
20 25 30
Glu Leu Ala Asn Ile Ile Gly His His Ala Gly Ile Asp Asp Asn Thr
35 40 45
Ala Ala Lys Ala Ile Ala His Ala Ile Leu Gly Gly Val Thr Ala Ala
50 55 60
Leu Gln Gly Asn Ser Ala Ala Ala Gly Ala Ile Gly Ala Gly Thr Gly
65 70 75 80
Glu Val Ile Ala Ser Ala Ile Ala Lys Ser Leu Tyr Pro Gly Val Asp
85 90 95
Pro Ser Lys Leu Thr Glu Asp Gln Lys Gln Thr Val Ser Thr Leu Ala
100 105 110
Thr Leu Ser Ala Gly Met Ala Gly Gly Ile Ala Ser Gly Asp Val Ala
115 120 125
Gly Ala Ala Ala Gly Ala Gly Ala Gly Lys Asn Val Val Glu Asn Asn
130 135 140
Ala Leu Ser Leu Val Ala Arg Gly Cys Ala Val Ala Ala Pro Cys Arg
145 150 155 160
Thr Lys Val Ala Glu Gln Leu Leu Glu Ile Gly Ala Lys Ala Gly Met
165 170 175
Ala Gly Leu Ala Gly Ala Ala Val Lys Asp Met Ala Asp Arg Met Thr
180 185 190
Ser Asp Glu Leu Glu His Leu Ile Thr Leu Gln Met Met Gly Asn Asp
195 200 205
Glu Ile Thr Thr Lys Tyr Leu Ser Ser Leu His Asp Lys Tyr Gly Ser
210 215 220
Gly Ala Ala Ser Asn Pro Asn Ile Gly Lys Asp Leu Thr Asp Ala Glu
225 230 235 240
Lys Val Glu Leu Gly Gly Ser Gly Ser Gly Thr Gly Thr Pro Pro Pro
245 250 255
Ser Glu Asn Asp Pro Lys Gln Gln Asn Glu Lys Thr Val Asp Lys Leu
260 265 270
Asn Gln Lys Gln Glu Ser Ala Ile Lys Lys Ile Asp Asn Thr Ile Lys
275 280 285
Asn Ala Leu Lys Asp His Asp Ile Ile Gly Thr Leu Lys Asp Met Asp
290 295 300
Gly Lys Pro Val Pro Lys Glu Asn Gly Gly Tyr Trp Asp His Met Gln
305 310 315 320
Glu Met Gln Asn Thr Leu Arg Gly Leu Arg Asn His Ala Asp Thr Leu
325 330 335
Lys Asn Val Asn Asn Pro Glu Ala Gln Ala Ala Tyr Gly Arg Ala Thr
340 345 350
Asp Ala Ile Asn Lys Ile Glu Ser Ala Leu Lys Gly Tyr Gly
355 360 365
<210>36
<211>128
<212>PRT
<213>大肠杆菌
<400>36
Met Ile Thr Leu Arg Lys Leu Ile Gly Asn Ile Asn Met Thr Lys Glu
1 5 10 15
Pro Glu Gln Gln Ser Pro Leu Glu Leu Trp Phe Glu Arg Ile Ile Asp
20 25 30
Val Pro Leu Glu Lys Leu Thr Val Glu Asp Leu Cys Arg Ala Ile Arg
35 40 45
Gln Asn Leu Cys Ile Asp Gln Leu Met Pro Arg Val Leu Glu Val Leu
50 55 60
Thr Lys Glu Pro Leu Ala Gly Glu Tyr Tyr Asp Gly Glu Leu Ile Ala
65 70 75 80
Ala Leu Ser Thr Ile Lys Gly Glu Asp Leu Lys Asp Gln Lys Ser Thr
85 90 95
Phe Thr Gln Ile Arg Gln Leu Ile Asn Gln Leu Glu Pro Ser Asp Ile
100 105 110
Asn Asp Asp Leu Arg Lys Asp Ile Leu Lys Ile Asn Gln Ile Ile Val
115 120 125
<210>37
<211>107
<212>PRT
<213>大肠杆菌
<400>37
Met Val Ala Lys Ala Phe Ala Tyr Ala Leu Asn Gln Trp Pro Ala Leu
1 5 10 15
Thr Tyr Tyr Ala Asn Asp Gly Trp Val Glu Ile Asp Asn Asn Ile Ala
20 25 30
Glu Asn Ala Leu Arg Ala Val Ser Leu Gly Arg Lys Asn Phe Leu Phe
35 40 45
Phe Gly Ser Asp His Gly Gly Glu Arg Gly Ala Leu Leu Tyr Ser Leu
50 55 60
Ile Gly Thr Cys Lys Leu Asn Asp Val Asp Pro Glu Ser Tyr Leu Arg
65 70 75 80
His Val Leu Ala Val Ile Ala Asp Trp Pro Val Asn Arg Val Ser Glu
85 90 95
Leu Leu Pro Trp Arg Ile Ala Leu Pro Ala Glu
100 105
<210>38
<211>86
<212>PRT
<213>大肠杆菌
<400>38
Met Leu Met Ser Val Gln Lys Glu Lys Asn Val Ala Glu Ser Val Val
1 5 10 15
Ser Glu Thr His Thr Gly Asp Ser Val Tyr Ala Ser Leu Phe Glu Lys
20 25 30
Ile Asn Leu Asn Pro Val Ser Ala Leu Ser Ala Leu Asp Asn Pro Phe
35 40 45
Arg Ser Ala Asp Asn Ala Thr Gly Arg Ile Thr Ser Ser Ile Gln Pro
50 55 60
Ala Val Gln Cys Ala Ala Ala Ala Ala Thr Glu Gly Ser Cys Pro Arg
65 70 75 80
Gln Ser Pro Cys Ser Gly
85
<210>39
<211>111
<212>PRT
<213>大肠杆菌
<400>39
Met Val Asp Asn Trp Gln Lys Ser Val Arg Ser Arg Ala Leu Pro Glu
1 5 10 15
Glu Ala Met Thr Gly Trp Asn Glu Gly Met Ile Arg Leu Gln Gln Leu
20 25 30
Ala Glu Arg Leu Asn Arg Gln Asp Glu Gln Arg Gly Lys Tyr Met Thr
35 40 45
Val Ser Glu Leu Lys Thr Glu Val Phe Gly Ile Met Gln Ala Phe Asn
50 55 60
Arg His Ile Pro Ala Glu Glu Gln Leu Arg Arg Tyr Gly Glu Val Arg
65 70 75 80
Asn Gln Asn Gly Ser Glu Gln Gln Gln Lys Gln Ala Glu Met Ala Leu
85 90 95
Asn Gln Leu Ile Asn Arg Tyr Gln Met Ile Arg Ala Gly Lys Gln
100 105 110
<210>40
<211>143
<212>PRT
<213>大肠杆菌
<400>40
Met Val Gly Cys Ala Trp Leu Ala Glu Gln Ala Phe Ser Asp His Ala
1 5 10 15
Leu Ser Pro His Ser Ala Trp Pro Tyr Ser Ala Ser Arg Asp Ala Gly
20 25 30
Leu Ala Asp Thr Gly Ala Gly Gly Tyr Pro Thr Cys Lys Gln Arg Trp
35 40 45
Ala Asp Asp Thr Val Gly Leu Lys Ala Arg Leu Leu Gln Leu Pro Ala
50 55 60
Leu Asp Ile Trp Thr Ala Phe Lys Lys Ile Asp Gln Ser Gln Val Val
65 70 75 80
Tyr Glu Glu Ala Val Leu Arg Ser Arg Val Ser Glu Arg Asn Met Gln
85 90 95
Val Ser Gln Asn Gly Arg Val Tyr Pro Ser Tyr Gly Gly Asn Val Asp
100 105 110
Gly Thr Val Ala Asn Ala Ala Thr Arg Leu Ala Ser Gly Ala Arg Asn
115 120 125
Ile Leu Gly Ser Ile Ala Ala Cys Thr Ala Phe Asp Ser Val Arg
130 135 140
<210>41
<211>118
<212>PRT
<213>大肠杆菌
<400>41
Met Val Gln Ala Gln Leu Gln Ile Ala Leu Val Ile Cys Ile Pro Leu
1 5 10 15
Ile Thr Leu Cys Ser Ala Trp Asp Val Lys Val Val Met Thr Leu Thr
20 25 30
Phe Val Gln Phe Ala Leu Phe Phe Leu Thr Phe Trp Trp Glu Leu Ala
35 40 45
Arg Trp Leu Asp Ser Trp Leu Leu Asp Val Leu Tyr Asn Ser Asp Thr
50 55 60
His Ser Ser Trp Asn Leu Ala Gly Ile Gln Asn Thr Gln Asp Asp Val
65 70 75 80
Ile Ile Asn Leu Val Met Arg Leu Met Phe Leu Val Leu Pro Thr Phe
85 90 95
Trp Leu Gly Ala Met Thr Trp Ala Gly Val Arg Val Gly Val Ala Leu
100 105 110
Asn Gly Ala Leu Ala Gly
115
<210>42
<211>81
<212>PRT
<213>大肠杆菌
<400>42
Met Lys Tyr Leu Phe Phe Glu Asn Ile His Ser Ile Phe Leu Thr Phe
1 5 10 15
Ser Leu Phe Arg Thr Ser Val Ser Pro Asp Phe Pro Met Ile Phe Ala
20 25 30
Leu Pro Ser Ile Ile Leu Gly Gln Phe Thr Thr Asn Gln Leu Thr Asn
35 40 45
Phe Val Ile Cys Met Gly Asn Thr Val Glu Arg Arg Leu Gly Val Val
50 55 60
His Asn Pro Phe Lys Arg Ser Gly Asp Gly His Asp Leu Arg Ala Val
65 70 75 80
Ala
<210>43
<211>348
<212>PRT
<213>大肠杆菌
<400>43
Leu Ile Val Ile Asp Phe Phe Cys Gly Cys Gly Gly Ala Ser Glu Gly
1 5 10 15
Leu Arg Gln Ala Gly Phe Asp Ile Glu Leu Gly Leu Asp Ile Asp Gln
20 25 30
Gln Ala Ser Glu Thr Phe Lys Ala Asn Phe Pro Asp Ala Lys Phe Ile
35 40 45
Gln Asp Asp Ile Arg Lys Ile Glu Pro Gln Asp Ile Ser Asp Ile Ile
50 55 60
Asp Ile Lys Ala Lys Arg Pro Leu Leu Leu Ser Ala Cys Ala Pro Cys
65 70 75 80
Gln Pro Phe Ser Gln Gln Asn Lys Asn Lys Thr Ser Asp Asp Ser Arg
85 90 95
Arg Asn Leu Leu Asn Glu Thr His Arg Phe Ile Arg Glu Leu Leu Pro
100 105 110
Glu Tyr Ile Met Leu Glu Asn Val Pro Gly Met Gln Lys Ile Asp Glu
115 120 125
Glu Lys Glu Gly Pro Phe Gln Glu Phe Ile Lys Leu Leu Lys Glu Leu
130 135 140
Glu Tyr Asn Tyr Ile Ser Phe Ile Ala Asn Ala Glu Asn Tyr Gly Ile
145 150 155 160
Pro Gln Arg Arg Lys Arg Leu Val Leu Leu Ala Ser Arg Val Gly Lys
165 170 175
Val Thr Leu Pro Glu Ile Thr His Gly Lys Asn Lys Ile Pro Phe Lys
180 185 190
Thr Val Arg Asp Tyr Ile Gln Asp Phe Thr Lys Leu Cys Ser Gly Glu
195 200 205
Thr Asp Pro Lys Asp Pro Leu His Arg Ala Gly Thr Leu Ser Pro Leu
210 215 220
Asn Leu Lys Arg Ile Met His Thr Pro Glu Gly Gly Asp Arg Arg Asn
225 230 235 240
Trp Pro Glu Glu Leu Val Asn Lys Cys His Lys Asn Tyr Asp Gly His
245 250 255
Thr Asp Thr Tyr Gly Arg Met Ser Trp Asp Lys Pro Ala Pro Thr Leu
260 265 270
Thr Thr Lys Cys Asn Ser Tyr Ser Asn Gly Arg Phe Gly His Pro Asp
275 280 285
Pro Thr Gln His Arg Ala Ile Ser Ile Arg Glu Ala Ser Arg Leu Gln
290 295 300
Thr Phe Pro Leu Ser Tyr Val Phe Lys Gly Ser Leu Asn Ser Met Ala
305 310 315 320
Lys Gln Ile Gly Asn Ala Val Pro Cys Glu Leu Ala Arg Leu Phe Gly
325 330 335
Leu His Leu Ile Glu Asn Cys Thr Asn Lys Asp Ser
340 345
<210>44
<211>974
<212>PRT
<213>大肠杆菌
<400>44
Met Leu Gly Arg Gln Gln Ile Ala Gly Ile Pro Thr Ala Leu Ser Glu
1 5 10 15
Leu Phe Lys Asn Ala His Asp Ala Tyr Ala Asp Asn Val Glu Val Asp
20 25 30
Phe Phe Arg Lys Glu Asn Leu Leu Ile Leu Arg Asp Asp Gly Leu Gly
35 40 45
Met Thr Thr Asp Glu Phe Glu Glu Arg Trp Leu Thr Ile Gly Thr Ser
50 55 60
Ser Lys Leu Ile Asp Asp Asp Ala Ile Asn Lys Pro Ala Val Asp Ser
65 70 75 80
Asn Lys Ala Phe Arg Pro Ile Met Gly Glu Lys Gly Ile Gly Arg Leu
85 90 95
Ser Ile Ala Ala Ile Gly Pro Gln Val Leu Val Leu Thr Arg Ala Lys
100 105 110
Arg Asp Asn Glu Leu Lys Pro Leu Val Ala Ala Phe Val Asn Trp Ser
115 120 125
Leu Phe Ala Ile Pro Ser Leu Asp Leu Asp Asp Ile Glu Ile Pro Ile
130 135 140
Arg Thr Ile Ile Asn Asp Glu Cys Phe Thr Lys Lys Thr Leu Asp Glu
145 150 155 160
Met Ile Glu Gln Ala Arg Asn Asn Leu Asp Ser Leu Ser His Lys Ile
165 170 175
Ser Lys Ser Lys Val Ser Gln Ile Asn Thr Gln Leu Ser Ser Phe Glu
180 185 190
Phe Asp Pro Ile Leu Trp Glu Lys Lys Leu Gly Gly Leu Arg Leu Ser
195 200 205
Gly Asp Gly His Gly Thr His Phe Ile Ile Met Pro Thr Glu Glu Ile
210 215 220
Leu Ile Asp Asp Ile Ser Thr Ser Asp Sar Asn Lys Thr Ser Glu Gln
225 230 235 240
Ser Ser Arg Leu Glu Lys Ala Leu Leu Gly Phe Thr Asn Thr Met Tyr
245 250 255
Ser Asp Ser Asn Pro Pro Ile Ile Ala Arg Phe Arg Asp Tyr Leu Glu
260 265 270
Asp Gly Glu Cys Ile Asp Arg Ile Ser Glu Ser Ile Phe Phe Thr Pro
275 280 285
Gln Glu Phe Asn Leu Ala Asp His His Ile Glu Gly Trp Phe Asn Glu
290 295 300
Phe Gly Gln Phe Ser Gly Thr Val Ser Val Tyr Gly Glu Glu Pro Ile
305 310 315 320
His His Val Val Thr Trp Lys Asn Asn Asn Gln Leu Thr Gln Cys Gly
325 330 335
Pro Phe Lys Ile Lys Leu Ala Tyr Ile His Gly Arg Leu Arg Asp Ser
340 345 350
Arg Leu Pro Met Glu Leu Trp Ala Pro Leu Lys Glu Lys Thr Asp Arg
355 360 365
Tyr Gly Gly Leu Tyr Ile Tyr Arg Asp Gly Leu Arg Ile Leu Pro Tyr
370 375 380
Gly Asp Ser Asp Thr Asp Phe Leu Lys Ile Glu Lys Arg Arg Thr Leu
385 390 395 400
Ser Ala Ser Glu Tyr Phe Phe Ser Tyr Arg Arg Leu Phe Gly Ala Ile
405 410 415
Glu Leu Thr Lys Glu Asn Asn Ala Ser Leu Val Glu Lys Ala Gly Arg
420 425 430
Glu Gly Phe Ile Glu Asn Lys Pro Tyr Lys Gln Phe Lys Glu Met Leu
435 440 445
Glu Asn Phe Phe Ile Glu Ile Ala Arg Asp Phe Phe Lys Asp Asp Gly
450 455 460
Asp Met Ser Glu Leu Phe Val Glu Thr Lys Gln Arg Arg Asn Glu Glu
465 470 475 480
His Asp Leu Leu Ser Lys Arg Ser Lys Gln Thr Lys Ala Lys Lys Asp
485 490 495
Arg Leu Lys Lys Asp Leu Tyr Asp Phe Phe Asp Lys Leu Asp Asn Asp
500 505 510
Tyr Trp Asn Ile Glu Ile Asn Lys Leu Ile Asn Lys Asn Glu Glu Tyr
515 520 525
Phe Ser Ser Thr Glu Ile Thr Asp Thr Asn Ile Asp Tyr Val Tyr Asn
530 535 540
Lys Ile Lys Glu Gln Asn Asp Ala Ile Ile Lys Asn Leu Arg Asn Ser
545 550 555 560
Val Asp Ile Lys Lys Pro Ser Gly Val Gly Leu Thr Lys Glu Leu Ser
565 570 575
Asn Leu Trp Asp Arg Tyr Gln Ile Glu Arg Gln Lys Ile Leu Leu Ser
580 585 590
Leu Asn Glu Leu Lys Asp Asn Val Asp Arg Lys Leu Ile Glu Leu Asp
595 600 605
Asn Lys Asn Asn Asp Phe Leu Asn Leu Arg Lys Arg Leu Glu Asp Ser
610 615 620
Leu Asn Leu Gln Gln Ser Tyr Tyr Glu Lys Glu Leu Thr Lys Leu Tyr
625 630 635 640
Asn Asp Ala Lys Asn Ala Leu Lys Asp Val Gln Ser Lys Ala Asn Arg
645 650 655
Leu Ile Ser Asp Asn Lys Lys Lys His Lys Ser Glu Leu Lys Asn Ile
660 665 670
Ser Tyr Glu Phe Gln Ser Thr Asn Leu Asn Gly Lys Asp Thr Ala Tyr
675 680 685
Ile Leu Asp Val Lys Arg Asn Leu Glu Ser Lys Ile Glu Asn Thr Ser
690 695 700
Asn Glu Val Ile Asn Glu Ile Arg Lys Leu Thr Asp Gln Ile Ala Ile
705 710 715 720
Ile Ser Asp Ser Thr Thr Ser Glu Asn Leu Ser Ser Ala Gln Val Thr
725 730 735
Glu Ala Ile Glu Thr Glu Leu Glu His Leu Arg Asp Gln Gln Ala Asn
740 745 750
Asn Ala Glu Leu Ile Leu Leu Gly Met Ala Leu Ser Val Val His His
755 760 765
Glu Phe Asn Gly Asn Ile Arg Ala Ile Arg Ser Ala Leu Arg Glu Leu
770 775 780
Lys Ala Trp Ala Asp Arg Asn Pro Lys Leu Asp Ile Ile Tyr Gln Lys
785 790 795 800
Ile Arg Thr Ser Phe Asp His Leu Asp Gly Tyr Leu Lys Thr Phe Thr
805 810 815
Pro Leu Thr Arg Arg Leu Ser Arg Ser Lys Thr Asn Ile Thr Gly Thr
820 825 830
Ala Ile Leu Glu Phe Ile Arg Asp Val Phe Asp Asp Arg Leu Glu Lys
835 840 845
Glu Gly Ile Glu Leu Phe Thr Thr Ser Lys Phe Val Asn Gln Glu Ile
850 855 860
Val Thr Tyr Thr Ser Thr Ile Tyr Pro Val Phe Ile Asn Leu Ile Asp
865 870 875 880
Asn Ala Ile Tyr Trp Leu Gly Lys Thr Thr Gly Glu Lys Arg Leu Ile
885 890 895
Leu Asp Ala Thr Glu Thr Gly Phe Val Ile Gly Asp Thr Gly Pro Gly
900 905 910
Val Ser Thr Arg Asp Arg Asp Ile Ile Phe Asp Met Gly Phe Thr Arg
915 920 925
Lys Thr Gly Gly Arg Gly Met Gly Leu Phe Ile Ser Lys Glu Cys Leu
930 935 940
Ser Arg Asp Gly Phe Thr Ile Arg Leu Asp Asp Tyr Thr Pro Glu Gln
945 950 955 960
Gly Ala Phe Phe Ile Ile Glu Pro Ser Glu Glu Thr Ser Glu
965 970
<210>45
<211>555
<212>PRT
<213>大肠杆菌
<400>45
Met Thr Ser Ser Thr Asp Phe His Lys Leu Ser Glu Asp Cys Val Arg
1 5 10 15
Arg Phe Leu His Ser Val Val Ala Val Asp Asp Asn Met Ser Phe Gly
20 25 30
Ala Gly Ser Asp Thr Phe Pro Thr Asp Glu Asp Ile Asn Ala Leu Val
35 40 45
Asp Pro Asp Asp Asp Pro Thr Pro Ile Ile Thr Ala Ser Ala Ser Pro
50 55 60
Arg Ile Glu Ser Thr Lys Ser Lys Ala Lys Val Lys Asn His Pro Phe
65 70 75 80
Asp Tyr Gln Ala Leu Ala Glu Ala Phe Ala Lys Asp Gly Ile Ala Cys
85 90 95
Cys Gly Leu Leu Ala Lys Ser Phe Asn Val Glu Glu Arg Asp Ile Ile
100 105 110
Thr Ala Ser Ser His Lys Ala Asp Ile Thr Ile Leu Asp Trp Asp Met
115 120 125
Gln Ser Asp Ser Gly Gln Phe Ala Ile Glu Ile Ile Lys Ser Ile Ile
130 135 140
Val Ser Asp Ile Asn Ser Gly Gly Arg Leu Arg Leu Leu Ser Ile Tyr
145 150 155 160
Thr Gly Glu His Val Thr Ala Val Ile Thr Lys Leu Asn Asn Glu Leu
165 170 175
Lys Lys Thr Tyr Arg Ser Val Ile Lys Asn Asp Asp Ser Ile Phe Ile
180 185 190
Glu Asp Asn Tyr Ala Leu Glu Gln Trp Cys Ile Val Val Ile Ser Lys
195 200 205
Asp Val Tyr Glu Lys Asp Leu Pro Asn Val Leu Ile Lys Lys Phe Thr
210 215 220
Asn Leu Thr Ala Gly Leu Leu Ser Asn Ala Ala Leu Ser Cys Ile Ser
225 230 235 240
Glu Ile Arg Glu Lys Thr His Gly Ile Leu Thr Lys Tyr Asn Asn Lys
245 250 255
Leu Asp Thr Ala Tyr Val Ser His Ile Leu Asn Leu Ile Lys Ser Lys
260 265 270
Glu Ser Arg Ala Tyr Ala Tyr Glu Asn Ala His Asp Tyr Ala Val Asp
275 280 285
Leu Ile Ser Glu Glu Ile Arg Ser Ile Leu Gln Ile Ser Glu Asn Leu
290 295 300
Lys Lys Ser Leu Ser Lys Asn Ser Leu Ser His Trp Pro Ile Phe His
305 310 315 320
Tyr Ala Lys Asn Gly Cys Lys Asn Phe Leu Leu Thr Gly Lys Lys Gln
325 330 335
Lys Asp Leu Ser Val Glu His Leu Arg Asn Ile Leu Ser Ala Asp Ser
340 345 350
Leu Glu Glu Ile Gln His Ala Ile Glu His Ala Ser Leu Gly Lys Lys
355 360 365
Glu Tyr Leu Ser Gln Asp Gly Glu Glu Asp Lys Lys Leu Met Gln Leu
370 375 380
Cys Ser Leu Glu Ile Thr Arg Arg Ser Leu Arg Tyr His Ser His Ile
385 390 395 400
Asp Asn Val Ser Leu Lys Gln Gly Thr Leu Leu Leu Asp Ala Tyr Asn
405 410 415
Phe Val Tyr Leu Cys Ile Gln Pro Leu Cys Asp Ser Val Arg Leu His
420 425 430
Glu Lys Ala Asp Phe Leu Phe Leu Arg Gly Thr Leu Asp Asp Asn Asn
435 440 445
Tyr Asn Leu Leu Ile Glu Asp Glu Tyr Gly Gly Phe Tyr Lys Ile Lys
450 455 460
Met Pro Ala Lys Ala Ser Asn Ile Ile Ser Phe Ser Phe Gly Val Glu
465 470 475 480
Asn Gly Asn Gly Val Ile Ile Gly Lys Lys Asn Asn Leu Val Asn Thr
485 490 495
Asp Tyr Ile Ser Phe Val Pro Leu Leu Val Glu Lys Ile Ser Thr Pro
500 505 510
Lys Val Leu Lys Trp Ile Gly Glu Ile Lys Thr Thr Tyr Ala Gln Lys
515 520 525
Ile Thr Thr Asp Ile Val Ala Asn Leu Ser Arg Ile Gly Leu Asp Gln
530 535 540
His Glu Trp Leu Arg Ile Lys Ser Lys Asp Ile
545 550 555
<210>46
<211>82
<212>PRT
<213>大肠杆菌
<400>46
Met Ser Ser Arg Gln Ile Leu Glu His Tyr Asn Ala Leu Thr Tyr Pro
1 5 10 15
Leu His Gln Ser Ile Leu Leu Gln Ile Met Thr Ser Asn Leu Leu Ser
20 25 30
Val Cys Thr Gly Lys Ser Ile Tyr Glu Asp Ile Ser Gly Ser Ser Trp
35 40 45
Asn Ile Ile His Phe Asn Ile Pro Leu Pro Ile Ser Arg Ala Arg Leu
50 55 60
Ser Ile Phe Ser Tyr Cys Val Arg Ile Lys Pro Trp Met Ser Met Asp
65 70 75 80
Tyr Met
<210>47
<211>98
<212>PRT
<213>大肠杆菌
<400>47
Met Ser Ile Ile Phe Asn Gly His Tyr Arg Met Lys His Arg Thr Trp
1 5 10 15
Ile Thr Glu Ala Leu Arg Leu His Phe Glu Glu His Leu Pro Gln Val
20 25 30
Val Val Gly Arg Arg Leu Gly Val Pro Lys Ser Thr Ala Cys Gly Met
35 40 45
Phe Val Arg Phe Arg Lys Ala Gly Phe Ser Trp Pro Leu Pro Ala Gly
50 55 60
Met Ser Glu Arg Glu Leu Asp Gly Arg Leu Tyr Gly Ser Thr Ser Thr
65 70 75 80
Val Pro Val Val Leu Cys Ser Gly Ser Val Ile Gln Asp Thr Ser Lys
85 90 95
Ser Cys
<210>48
<211>106
<212>PRT
<213>大肠杆菌
<400>48
Met Ile Lys Thr Arg Arg Thr Lys Arg Thr Phe Ser Pro Glu Phe Lys
1 5 10 15
Leu Glu Ala Phe Glu Gln Val Val Val Lys Tyr Gln Arg Asp Val Arg
20 25 30
Glu Val Ala Gln Ala Leu Glu Leu Asn Pro Asp His Leu Arg Lys Trp
35 40 45
Ile Arg Leu Tyr Lys Gln Glu Leu Gln Gly Ile Glu Pro Ala Gly Asn
50 55 60
Ala Ile Thr Pro Glu Gln Arg Glu Ile Gln Gln Leu Lys Ala Gln Ile
65 70 75 80
Lys Arg Val Glu Met Glu Lys Glu Ile Leu Lys Gln Ala Ala Val Leu
85 90 95
Met Ser Glu Ile Pro Gly Lys Leu Ser Arg
100 105
<210>49
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>49
tgctctagag ccattactca gaatggg 27
<210>50
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>50
cgcgagctcg acgactgaat gatccc 26
<210>51
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>51
tcccccgggt actgcagcac tcaacc 26
<210>52
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>52
gatcccggga ccactgaaat gcgtgc 26
<210>53
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>53
tcgtctagag atgatggtga tggagcg 27
<210>54
<211>28
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>54
gaactgcagc caaatactga taccaccc 28
<210>55
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>55
gaactgcagg ctaaaacaga agacgcg 27
<210>56
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>56
catgcatgca ctccatatga caaccgc 27
<210>57
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>57
tcgtctagaa tgaagctgcg catgagg 27
<210>58
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>58
caactgcagt cgcaaattgc gaactgg 27
<210>59
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>59
caactgcaga ccgcaacttt tcgacgc 27
<210>60
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>60
catgcatgcc agtgagccat tgttccc 27
<210>61
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>61
tgctctagat acgactctga caggagg 27
<210>62
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>62
tcagatatca actaccagca gtttgg 26
<210>63
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>63
tcagatcc ataaagagtg acgtggc 27
<210>64
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>64
tgctctagaa aacgtggcaa cagagcg 27
<210>65
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>65
tgctctagaa ggcgttgtcg atcctg 26
<210>66
<211>28
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>66
gaactgcagg aaaaggccga gcagactg 28
<210>67
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>67
gaactgcagt acagccatgt ttacggt 27
<210>68
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>68
catgcatgcg gtgtacgaca gtttgcg 27
<210>69
<211>26
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>69
tgctctagac acatcatggg cacacc 26
<210>70
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>70
gaactgcaga accgtccaca tcaggcg 27
<210>71
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>71
gaactgcaga ccctgcttgc cattccg 27
<210>72
<211>27
<212>DNA
<213>人工序列
<220>
<223>人工序列描述：寡核苷酸
<400>72
catgcatgca taagcgtcga acaggcg 27