产生GH8木聚糖酶变体的方法本发明申请是基于申请日为2010年12月9日，申请号为201080063420.4(国际申请
号为PCT/EP2010/069249)，名称为“产生GH8木聚糖酶变体的方法”的发明专利申请的分案
申请。
涉及序列表
本申请包含计算机可读形式的序列表。所述计算机可读形式通过提述并入本文。
发明领域
本发明涉及产生保留木聚糖酶活性的亲本GH8木聚糖酶多肽的变体的方法。本发
明还涉及具有木聚糖酶活性的分离的GH8多肽变体和编码所述多肽变体的分离的多核苷
酸。本发明进一步涉及包含所述多核苷酸的核酸构建体、载体和宿主细胞，以及用于产生和
使用所述多肽的方法，如，制备生面团或从这种生面团制备的烘烤食物。更具体而言，本发
明涉及这样的工艺，其中所述生面团与用亲本GH8木聚糖酶制备相比，粘性较低，和/或其中
面包具有增加的体积。
发明背景
糖和二醇缀合物(glycol-conjugate)是糖基转移酶(GT)和糖苷水解酶(GH)的底
物。糖苷水解酶的结构在二十世纪八十年代逐渐开始的得到解析。与此同时，发现了新GH蛋
白，并确定了其氨基酸序列。从这些新数据，得到了两个主要的观察结果。1)用于命名酶家
族的经典的E.C.命名系统不够精确至能够对越来越多的具有不同结构但实现相同酶反应
的酶进行分类。2)通过同源性相关联的酶可具有不同的酶活性，因此亦使得E.C.命名系统
对于这些相关的酶是易混淆的。
在1991年，由Bernard Henrissat基于酶结构提出了新的基于家族的命名系统
(Henrissat B.,A classification of glycosyl hydrolases based on amino-acid
sequence similarities.Biochem.J.280:309-316(1991)；Henrissat B.,Bairoch A.New
families in the classification of glycosyl hydrolases based on amino-acid
sequence similarities.Biochem.J.293:781-788(1993)；Henrissat B.,Bairoch
A.Updating the sequence-based classification of glycosyl
hydrolases.Biochem.J.316:695-696(1996)以及Davies G.,Henrissat B.Structures
and mechanisms of glycosyl hydrolases.Structure 3:853-859(1995)。最新的分类系
统可从Carbohydrate-Active EnZymes网站获得(CAZy)。
基于氨基酸序列相似性将糖苷水解酶归类为家族的分类的导入是因为序列和折
叠类似性之间有直接关系，而预期此种分类会：
(i)反映酶的结构特征，这无法仅通过底物特异性反映，
(ii)协助揭示酶之间的进化关系，和
(iii)提供用于得到机理信息的方便工具。
将氨基酸序列根据其与特定GH家族的相似性分组可给出针对新的假设蛋白的活
性方面的概念。这些氨基酸序列中的一些通过同源性归类为一个GH家族，后来表明其具有
某种酶活性。因此，简言之，将新氨基酸序列归类于一个GH家族并未具体表明确切的酶活
性。该酶活性必需通过克隆或纯化蛋白的活性测定来阐明。若该测定是困难的，蛋白实际功
能的确定可能多年仍无法揭示。
糖苷水解酶家族8(GH8)包含具有几种已知活性的酶：内切葡聚糖酶(EC:
3.2.1.4)；地衣聚糖酶(lichenase)(EC:3.2.1.73)；壳聚糖酶(EC:3.2.1.132)。
一些木聚糖酶受见于面粉中的组分所抑制，这使得它们不适用于制造基于生面团
的产物。GH8木聚糖酶不受生面团中的组分所抑制，且其在生面团中仅需要非常低剂量以提
供烘烤面包的体积增加。不幸的是，在烘烤领域中众所周知，使用任何目前为止鉴定出的天
然存在的GH8木聚糖酶得到粘性生面团，对其存在操作困难。
发明内容
发明人鉴定出了在广泛范围的GH8木聚糖酶中保守的特征性氨基酸基序，其包含
这些木聚糖酶中活性位点中的谷氨酸(E)亲核体：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1)。
使用了分子建模，并鉴定出沿GH8木聚糖酶活性位点线性排列的结构环(参见图
1)。该环包含保守的GH8基序N端侧直接侧翼的约20个连续氨基酸(图1)。
制造了合成表达构建体(SEQ ID NO:2)，其编码来自芽孢杆菌属菌种(Bacillus
sp.)KK-1(UNIPROT登录号O52730；SEQ ID NO:4)的成熟GH8木聚糖酶。该合成构建体包含编
码相互融合的成熟GH8木聚糖酶多肽编码序列和异源信号肽的序列，该构建体的核酸序列
就芽孢杆菌属表达进行了优化。编码的异源信号肽和成熟的GH8木聚糖酶的氨基酸序列示
于SEQ ID NO:3。SEQ ID NO:3和SEQ ID NO:4的成熟多肽的氨基酸序列不言自明是相同的。
保守的GH8基序见于SEQ ID NO:3或4中所示的成熟多肽的氨基酸位置65-70。
SEQ ID NO:3中保守GH8基序侧翼的环通过其中包含的氨基酸的定点诱变来进行
突变。进行了单个氨基酸的缺失、插入和取代，及其某些组合，以产生多种具有木聚糖酶活
性的GH8变体，参见下文。在烘烤应用中调查了变体的活性，且其显示令人惊讶的饶有兴趣
和有希望的特性。
相应地，在第一个方面，本发明涉及产生亲本GH8木聚糖酶多肽的变体的方法，所
述方法包括下述步骤：
a)提供编码成熟亲本GH8木聚糖酶多肽的多核苷酸，所述多肽包含第一保守GH8木
聚糖酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1)，
b)将至少一个突变导入编码多核苷酸，由此将一个改变在第一保守GH8木聚糖酶
基序N端侧侧翼的20个氨基酸位置中的一个或多个处导入编码的多肽，和
c)将突变的多核苷酸在合适宿主细胞中并在有助于产生变体的条件下进行表达，
其中所述变体具有木聚糖酶活性。
在第二个方面，本发明涉及亲本GH8木聚糖酶多肽的分离的变体，所述多肽包含第
一保守GH8木聚糖酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1)，
其中所述变体相对于亲本在第一保守GH8基序N端侧侧翼的20个氨基酸位置中的
一个或多个中包含至少一个氨基酸改变，且其中所述变体具有木聚糖酶活性。
在第三个方面，本发明涉及包含核苷酸序列的分离的多核苷酸，所述核酸序列编
码第二个方面的变体多肽。
第四个方面涉及包含第三个方面的多核苷酸的核酸构建体。
第五个方面涉及包含第四个方面的核酸构建体的重组表达载体。
第六个方面涉及重组宿主细胞，其包含前述方面的重组表达载体、第四个方面的
核酸构建体或可操作连接的第三个方面的多核苷酸和一个或多个调控序列，所述调控序列
指导变体多肽的产生。
第七个方面涉及产生第二个方面的变体多肽的方法，包括：(a)在有助于变体产生
的条件下，培养包含核酸构建体的宿主细胞，所述核酸构建体包含编码变体多肽的核苷酸
序列；和(b)回收所述变体。
其他方面涉及包含第二个方面的变体多肽的组合物，用于制备烘烤产品的方法
(所述方法包括将第二个方面的变体多肽添加至所述烘烤产品的生面团的步骤)，或包含第
二个方面的变体多肽的面包改善组合物。
本发明的另一个方面涉及用于增加烘烤产品的面包体积(loaf volume)的方法，
其包括在所述烘烤产品的生面团的混合过程中添加充足量的第二个方面的变体多肽或包
含所述多肽的组合物的步骤。
本发明的仍旧另一个方面涉及增加烘烤产品的面包体积的方法或增加烘烤产品
表面上的切痕(cut)宽度的方法，其包括在所述烘烤产品的生面团的混合过程中添加充足
量的第二个方面的变体多肽或包含所述多肽的组合物。
在最后一个方面，本发明涉及用于增加烘烤产品的面包体积或增加烘烤产品表面
上的切痕(cut)宽度的面包改善组合物，其特征在于其包含至少一种第二个方面的变体多
肽。
具体地，本发明涉及如下各项：
1.一种产生亲本GH8木聚糖酶多肽的变体的方法，所述方法包括下述步骤：
a)提供编码成熟亲本GH8木聚糖酶多肽的多核苷酸，所述多肽包含第一保守GH8木
聚糖酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1)，
b)将至少一个突变导入编码多核苷酸，由此将至少一个改变在第一保守GH8木聚
糖酶基序N端侧侧翼的20个氨基酸位置中的一个或多个处导入编码的多肽，和
c)将突变的多核苷酸在合适宿主细胞中并在有助于产生变体的条件下进行表达，
其中所述变体具有木聚糖酶活性。
2.项1的方法，其中所述成熟亲本GH8木聚糖酶多肽包含氨基酸序列，所述氨基酸
序列与选自SEQ ID NO:3至SEQ ID NO:154和SEQ ID NO:159中任一所示的GH8木聚糖酶的
组中的GH8木聚糖酶的成熟部分至少60％相同。
3.项1或2的方法，其中所述成熟亲本GH8木聚糖酶包含第二保守GH8木聚糖酶基
序：
[ND][AS]X[RLPQ] (SEQ ID NO:155)。
4.项1-3任一项的方法，其中所述成熟亲本GH8木聚糖酶包含或组成为SEQ ID NO:
3的氨基酸28至433。
5.项1-4任一项的方法，其中所述编码的多肽中的至少一个改变包括在第一保守
GH8木聚糖酶基序N端侧侧翼的20个氨基酸位置中的一个或多个处的一个或多个氨基酸的
取代、缺失和/或插入。
6.项1-5任一项的方法，其中所述编码多肽中的至少一个改变包括在第一保守GH8
木聚糖酶基序N端侧侧翼的15个氨基酸位置中的一个或多个处的一个或多个氨基酸的插
入；优选一个或多个丙氨酸的插入。
7.项1-6任一项的方法，其中所述变体当以充足量在混合之前或混合过程中加入
生面团时，具有至少一种面包改善特性。
8.项1-7任一项的方法，其中所述变体当以充足量在混合之前或混合过程中加入
生面团时，能够增加烘烤产品的面包体积或增加烘烤产品表面上切痕的宽度。
9.项7或8任一项的方法，其中所述变体当以充足量在混合之前或混合过程中加入
生面团时，与亲本GH8木聚糖酶相比，提供粘性较低的生面团。
10.一种亲本GH8木聚糖酶多肽的分离的变体，所述多肽包含第一保守GH8木聚糖
酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1),
其中所述变体与亲本相比在第一保守GH8基序N端侧侧翼的20个氨基酸位置中的
一个或多个处包含至少一个氨基酸改变，且其中所述变体具有木聚糖酶活性。
11.项10的变体，选自下述的变体组：
(a)变体多肽，其包含氨基酸序列，所述氨基酸序列与选自SEQ ID NO:3至SEQ ID
NO:154和SEQ ID NO:159中所示的GH8木聚糖酶的组中的GH8木聚糖酶的成熟部分具有至少
60％序列同一性；
(b)变体多肽，其由多核苷酸编码，所述多核苷酸在至少中等-高严格条件下与以
下杂交：(i)SEQ ID NO:2中所示的成熟多肽编码序列，或(ii)(i)的全长互补链；
(c)变体多肽，其由多核苷酸编码，所述多核苷酸包含核苷酸序列，所述核苷酸序
列与SEQ ID NO:2中所示的成熟多肽编码序列具有至少60％序列同一性；和
(d)(a)、(b)或(c)的变体，其包含一个或多个氨基酸的取代、缺失和/或插入。
12.项10或11的变体，其中所述亲本GH8木聚糖酶多肽包含第二保守GH8木聚糖酶
基序：[ND][AS]X[RLPQ](SEQ ID NO:155)。
13.项10-12任一项的变体，其中所述成熟亲本GH8木聚糖酶多肽包含氨基酸序列，
所述氨基酸序列与SEQ ID NO:3中所示的GH8木聚糖酶的成熟部分至少60％相同。
14.项10-13任一项的变体，其由多核苷酸编码，所述多核苷酸在至少中等-高严格
条件下与以下杂交：(i)多核苷酸，其包含SEQ ID NO:2的成熟多肽编码序列的核苷酸序列，
或(ii)(i)的全长互补链。
15.项10-14任一项的变体，其由多核苷酸编码，所述多核苷酸包含核苷酸序列，所
述核苷酸序列与SEQ ID NO:2的成熟多肽编码序列具有至少60％序列同一性。
16.项10-15任一项的变体，其由多核苷酸编码，所述多核苷酸包含或组成为SEQ
ID NO:2的核苷酸序列；或其编码具有木聚糖酶活性的多肽片段的亚序列。
17.项10-16任一项的变体，其中所述成熟多肽包含或组成为SEQ IDNO:3的氨基酸
28至433。
18.项10-17任一项的变体，其中所述成熟多肽由多核苷酸编码，所述多核苷酸包
含或组成为SEQ ID NO:2的核苷酸82至1299。
19.项10-18任一项的变体，其中所述至少一个氨基酸改变包含一个或多个氨基酸
的取代、缺失和/或插入。
20.项10-19任一项的变体，其中所述至少一个氨基酸改变包含在N端侧距离第一
保守GH8木聚糖酶基序20，19，18，17，16，15，14，13，12，11，10，9，8，7，6，5，4，3，2和/或1个氨
基酸的一个或多个位置处，或在对应于SEQ ID NO:3的成熟多肽中的位置45，46，47，48，49，
50，51，52，53，54，55，56，57，58，59，60，61，62，63和/或64的一个或多个位置处的一个或多
个氨基酸的取代、缺失和/或插入。
21.项10-20任一项的变体，其中所述至少一个氨基酸改变包含于第一保守GH8木
聚糖酶基序N端侧侧翼的15个氨基酸位置中的一个或多个中，优选包含于第一保守GH8木聚
糖酶基序N端侧侧翼的12个氨基酸位置中的一个或多个中。
22.项10-21任一项的变体，其中所述至少一个氨基酸改变包含在对应于SEQ ID
NO:3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序12个氨基酸的位置的位置处，或
在对应于SEQ ID NO:3的成熟多肽中的位置53的位置处的一个或多个插入。
23.项10-21任一项的变体，其中所述至少一个氨基酸改变包含在对应于SEQ ID
NO:3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序10个氨基酸的位置的位置处，或
在对应于SEQ ID NO:3的成熟多肽中的位置55的位置处的一个或多个插入。
24.项10-21任一项的变体，其中所述至少一个氨基酸改变包含在对应于SEQ ID
NO:3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序8个氨基酸的位置的位置处，或
在对应于SEQ ID NO:3的成熟多肽中的位置57的位置处的一个或多个插入。
25.项10-21任一项的多肽，其中所述至少一个氨基酸改变包含在对应于SEQ ID
NO:3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序6个氨基酸的位置的位置处，或
在对应于SEQ ID NO:3的成熟多肽中的位置59的位置处的一个或多个插入。
26.项10-21任一项的变体，其中所述至少一个氨基酸改变包含在对应于SEQ ID
NO:3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序4个氨基酸的位置的位置处，或
在对应于SEQ ID NO:3的成熟多肽中的位置61的位置处的一个或多个插入。
27.项21-26任一项的变体，其中所述一个或多个插入包含至少一个丙氨酸残基，
至少一个天冬氨酸残基，至少一个丝氨酸残基，至少一个赖氨酸残基，和/或至少一个苯丙
氨酸的插入；优选所述一个或多个插入包含至少一个丙氨酸残基的插入。
28.项21-27任一项的变体，其中所述一个或多个插入包含至少一个氨基酸残基和
至少一个额外的但不同的氨基酸残基在相同位置或在不同位置的插入。
29.项10-28任一项的变体，当其以充足量在混合之前或在混合过程中添加至生面
团时具有至少一种面包改善特性。
30.项10-28任一项的变体，当其以充足量在混合之前或混合过程中添加至生面团
时，能够增加烘烤产品的面包体积，或增加烘烤产品表面的切痕的宽度。
31.项29或30的变体，当将其与以相同量添加的亲本GH8木聚糖酶相比时，其提供
了粘性较低的生面团。
32.一种分离的多核苷酸，其包含核苷酸序列，所述核苷酸序列编码项10-31任一
项的变体多肽。
33.一种核酸构建体，其包含可操作连接的项32的多核苷酸和一个或多个调控序
列，所述调控序列指导所述变体多肽在表达宿主中的产生。
34.一种重组表达载体，其包含项33的核酸构建体。
35.一种重组宿主细胞，其包含项34的重组表达载体、项33的核酸构建体或可操作
连接的项32的多核苷酸和一个或多个调控序列，所述调控序列指导所述变体多肽的产生。
36.一种产生项10-31任一项的变体多肽的方法，包括：(a)在有助于产生所述变体
的条件下培养包含核酸构建体的宿主细胞，所述核酸构建体包含编码所述变体多肽的核苷
酸序列；和(b)回收所述变体。
37.一种组合物，其包含项10-31任一项的变体多肽。
38.一种用于制备烘烤产品的方法，所述方法包括将项10-31任一项的变体多肽或
包含项10-31任一项的变体多肽的面包改善组合物添加至所述烘烤产品的生面团的步骤。
39.前述任一项项的方法，其中所述变体多肽或面包改善组合物在生面团的混合
过程中添加。
40.项38或39的方法，其中所述面包改善组合物进一步包含一种或多种选自下列
的面包改善剂：酶、乳化剂、氧化剂、奶粉、脂肪、糖、氨基酸、盐、蛋白(谷蛋白，纤维素结合位
点)或其混合物。
41.项40的方法，其中所述酶选自下列：α-淀粉酶、β-淀粉酶、产麦芽糖淀粉酶、其
他木聚糖酶、蛋白酶、葡糖氧化酶、氧化还原酶、葡聚糖酶、纤维素酶、转谷氨酰胺酶、异构
酶、脂肪酶、磷脂酶、果胶酶或其混合物。
42.项38-41任一项的方法，其中所述变体多肽作为细胞提取物、无细胞提取物或
纯化蛋白存在。
43.项38-41任一项的方法，其中将所述变体多肽与干粉或颗粒，特别是无尘颗粒
形式，或液体形式的其他成分混合，优选与一种或多种稳定剂如多元醇、糖、有机酸或糖醇
混合。
44.一种增加烘烤产品的面包体积的方法，包括在混合所述烘烤产品的生面团的
过程中，添加充足量的项10-31任一项的变体多肽或项37的组合物的步骤。
45.一种用于增加烘烤产品的面包体积或用于增加烘烤产品表面上切痕的宽度的
方法，包括在混合所述烘烤产品的生面团的过程中，添加充足量的项10-31任一项的变体多
肽或项37的组合物的步骤。
46.一种用于增加烘烤产品的面包体积或用于增加烘烤产品表面上切痕的宽度的
面包改善组合物，其特征在于其包含至少一种项10-31任一项的变体多肽。
附图说明
图1(横向)显示来自芽孢杆菌属菌种KK-1(UNIPROT登录号O52730；SEQ ID NO:4)
的亲本GH8木聚糖酶的分子模型的切面。提供了底物结合袋的特写(浅灰色网状)，其中活性
位点(深灰色网状)略低于图像中心。第一保守GH8木聚糖酶基序(未显示)N端侧侧翼的环
(深灰卷曲)见于活性位点略上方，可能略伸入底物结合袋。指示了三个环氨基酸残基53
(Asp)、55(Asn)和59(Gly)(深灰)，并显示了底物袋中底物分子的棒状模型。
图2(横向)显示如下文定义部分显示的计算出的木聚糖酶活性标准曲线，x轴为以
FXU/ml计的木聚糖酶活性，而y轴为在595nm处的吸光度(Abs595或OD595nm)。
定义
木聚糖酶活性：就本发明而言，任何商业上可获得的木聚糖酶活性测量试剂盒适
用于确定木聚糖酶活性。本发明中木聚糖酶活性测量的唯一目的是作为初级筛选以确保本
发明的GH8木聚糖酶变体并不会由于疏忽而被导入其中的氨基酸变化所失活。一种合适的
测量木聚糖酶活性的方式如下所示：
底物：
来自小麦的AZCL-阿拉伯木聚糖(Megazyme)。
测定缓冲液：
50mM B&R(50mM H3PO4，50mM乙酸，50mM H3BO3)，50mM KCl，1mM CaCl2，0.01％
Triton X-100，pH使用NaOH调整至6.0。
酶：
所有酶稀释在含0.01％Triton X-100的蒸馏水中制备。
底物溶液：
在测定缓冲液中制备AZCL-阿拉伯木聚糖底物的0.2％(w/v)浆料。切割1000μl移
液头的尖端以虹吸均一的等分试样。
标样：
BioFeed WheatTM(Novozymes A/S)，稀释于0.01％Triton X-100。FXU/ml：0.05；
0.10；0.15；0.20；0.25；0.30；0.40。
分析：
1.在Eppendorf热混合器中在37℃(混合)。
2.将750μl底物溶液置于冰上5分钟。
3.添加50μl酶样品。
4.在1400rpm，37℃温育15分钟。
5.在冰/水浴上终止反应。
6.在10,000rpm，4℃离心5分钟。
7.将200μl上清转移至微滴定板。
8.测量终点吸光度，OD595nm。
备注：吸光度OD595nm应在0-1.5的范围。
备注：在该测定中，从FXU标样获得标准曲线，但测量的活性为木聚糖酶单位。对
azo-木聚糖而非azcl-木聚糖测量FXU。
标准曲线：
从具有439FXU(W)/g的声明(declared)木聚糖酶活性的BioFeed WheatTM
(Novozymes A/S)制备标准溶液。将2.05g BioFeed WheatTM在0.9％NaCl中溶解至100mL，这
提供了具有9.0FXU/mL的活性的溶液(冻结的储液维持在-80℃)。
进行测定之前，即刻将储液稀释10倍以提供测定储备溶液(储液)。
计算：
标准曲线是从酶标样的A595减去std1获得的(如下所示)。
活性根据酶的A595减去空白值来计算。因为酶稀释液通常为无色的，在这些情况
下使用std1作为空白值。
标准曲线计算的实例，该曲线示于图2：
本发明的成熟变体多肽具有其成熟GH8亲本木聚糖酶多肽的至少20％，优选至少
40％，更优选至少50％，更优选至少60％，更优选至少70％，更优选至少80％，甚至更优选至
少90％，最优选至少95％，并且甚至最优选至少100％的木聚糖酶活性。
分离的多肽：术语“分离的多肽”用于本文中指从来源分离的多肽。在一个优选的
方面，所述多肽是至少1％纯，优选至少5％纯，更优选至少10％纯，更优选至少20％纯，更优
选至少40％纯，更优选至少60％纯，甚至更优选至少80％纯，和最优选至少90％纯，如通过
SDS-PAGE确定。
成熟多肽：术语“成熟多肽”在本文中定义为在翻译和任何翻译后修饰如N端加工、
C端截短、糖基化、磷酸化等之后以其最终形式存在的多肽。在一个方面，所述成熟多肽包含
或组成为表1中列出的氨基酸位置，参见下文。
成熟多肽编码序列：术语“成熟多肽编码序列”在本文中定义为编码具有木聚糖酶
活性的成熟多肽。在一个方面，所述成熟多肽编码序列包含或组成为编码成熟多肽的核苷
酸，所述成熟多肽包含或组成为表1中列出的氨基酸位置，参见下文。
序列同一性：参数“序列同一性”描述两个氨基酸序列之间或两个核苷酸序列之间
的相关性。
就本发明而言，两个氨基酸序列之间的序列同一性程度使用如EMBOSS软件包
(EMBOSS:The European Molecular Biology Open Software Suite,Rice等,2000,Trends
in Genetics 16:276-277)，优选3.0.0版或更高版本的Needle程序中所执行的Needleman-
Wunsch算法(Needleman和Wunsch,1970,J.Mol.Biol.48:443-453)来测定。使用的可选参数
为缺口产生罚分(gap open penalty)10，缺口延伸罚分(gap extension penalty)0.5和
EBLOSUM62(BLOSUM62的EMBOSS版)取代矩阵。使用Needle标记为“最长同一性(longest
identity)”的输出结果(使用-nobrief选项获得)作为同一性百分比，并计算如下：
(同样的残基×100)/(比对长度－比对中缺口的总数)
就本发明而言，两个脱氧核糖核苷酸序列之间的序列同一性程度使用如EMBOSS软
件包(EMBOSS:The European Molecular Biology Open Software Suite,Rice等,2000,见
上文)，优选3.0.0版或更高版本的Needle程序中所执行的Needleman-Wunsch算法
(Needleman和Wunsch,1970,见上文)来测定。使用的可选参数为缺口产生罚分10，缺口延伸
罚分0.5和EDNAFULL(NCBI NUC4.4的EMBOSS版)取代矩阵。使用Needle标记为“最长同一性”
的输出结果(使用-nobrief选项获得)作为同一性百分比，并计算如下：
(同样的脱氧核糖核苷酸×100)/(比对长度－比对中缺口的总数)
同源序列：术语“同源序列”在本文中定义为在用SEQ ID NO:3-154和159中任一所
示的任何多肽或其成熟部分进行的tfasty搜索(Pearson,W.R.,1999,于Bioinformatics
Methods and Protocols中,S.Misener和S.A.Krawetz编,pp.185-219)中E值(或期望分数)
小于0.001的预测蛋白质。
多肽片段：术语“多肽片段”在本文中定义为从SEQ ID NO:3-154和159中任一的成
熟多肽或其同源序列的氨基和/或羧基末端缺失一个或多个(几个)氨基酸的多肽；其中所
述片段具有木聚糖酶活性。
亚序列：术语“亚序列(subsequence)”在本文中定义为从成熟多肽编码序列或其
同源序列的5’和/或3’端缺失一个或多个(几个)核苷酸的核苷酸序列；其中所述亚序列编
码具有木聚糖酶活性的多肽片段。
等位变体(allelic variant)：术语“等位变体”在本文中表示占据相同染色体基
因座的基因的任何两种或更多种可选形式。等位变异通过突变天然地发生，并且可导致种
群内的多态性。基因突变可以是沉默的(在编码的多肽中无变化)或可以编码具有改变的氨
基酸序列的多肽。多肽的等位变体是由基因的等位变体编码的多肽。
分离的多核苷酸：术语“分离的多核苷酸”用于本文中指从来源分离的多核苷酸。
在一个优选的方面，多核苷酸如通过琼脂糖电泳测定的，为至少1％纯，优选至少5％纯，更
优选至少10％纯，更优选至少20％纯，更优选至少40％纯，更优选至少60％纯，甚至更优选
至少80％纯，并且最优选至少90％纯。
编码序列：当用于本文时术语“编码序列”的意思是直接指定其蛋白产物的氨基酸
序列的核苷酸序列。编码序列的边界通常由开读框决定，所述开读框通常以ATG起始密码子
或可供选择的起始密码子例如GTG和TTG开始，并且以终止密码子例如TAA、TAG和TGA结束。
编码序列可以是DNA、cDNA、合成的或重组的核苷酸序列。
cDNA：术语“cDNA”在本文中定义为能够通过反转录从得自真核细胞的成熟的、已
剪接的mRNA分子制备的DNA分子。cDNA缺少可能存在于相应基因组DNA中的内含子序列。起
始的(initial)、初级的RNA转录物是mRNA的前体，其通过一系列的步骤加工然后作为成熟
的已剪接的mRNA出现。这些步骤包括通过称为剪接的过程去除内含子序列。因而源自mRNA
的cDNA没有任何内含子序列。
核酸构建体：术语“核酸构建体”用于本文指单链或双链的核酸分子，其分离自天
然存在的基因或其受修饰以本来不存在于(not otherwise exist)自然界中的方式含有核
酸的区段，或其为合成的。当所述核酸构建体含有表达本发明的编码序列所需的调控序列
时，术语核酸构建体与术语“表达盒”同义。
调控序列(control sequence)：术语“调控序列”在本文定义为包括对编码本发明
多肽的多核苷酸表达是必需的所有成分。各个调控序列对于编码所述多肽的核苷酸序列可
以是天然的或外源的，或各个调控序列对于彼此可以是天然的或外源的。这些调控序列包
括但不限于前导序列、聚腺苷酸化序列、前肽序列、启动子、信号肽序列和转录终止子。最少
的情况，调控序列包括启动子和转录和翻译的终止信号。调控序列可以和用于引入特异性
限制位点的接头一起提供，所述特异性限制位点促进调控序列与编码多肽的核苷酸序列编
码区的连接。
可操作地连接：术语“可操作地连接”在本文表示这样的构型，其中将调控序列置
于相对于多核苷酸序列的编码序列的适当位置，使得调控序列指导多肽编码序列的表达。
表达：术语“表达”包括涉及多肽产生的任何步骤，其包括但不限于转录、转录后修
饰、翻译、翻译后修饰和分泌。
表达载体：术语“表达载体”在本文定义为线性的或环状的DNA分子，其包含编码本
发明多肽的多核苷酸，并且所述多核苷酸与提供用于其表达的额外核苷酸可操作地连接。
宿主细胞：如本文中所使用的术语“宿主细胞”包括任何细胞类型，所述细胞类型
对于使用包含本发明多核苷酸的核酸构建体或表达载体的转化、转染、转导等是易感的
(susceptible)。
修饰：术语“修饰”在本文的意思是，对包含SEQ ID NO:2的成熟多肽的多肽，或由
SEQ ID NO:2的成熟多肽组成的多肽或其同源序列的任何化学修饰，以及对编码所述多肽
的DNA的遗传操作。所述修饰可以是一个或多个(几个)氨基酸的取代、缺失和/或插入，以及
一个或多个(几个)氨基酸侧链的置换。
人工变体：当用在本文时，术语“人工变体”的意思是具有木聚糖酶活性的多肽，所
述多肽由表达修饰的多核苷酸序列的生物体产生，所述修饰的多核苷酸序列编码SEQ ID
NO:3-154和159中所示的任意多肽具有木聚糖酶活性的变体，或其具有木聚糖酶活性的成
熟部分的变体。所述修饰的核苷酸序列通过人为干预(human intervention)通过修饰编码
多核苷酸序列或完整合成编码所述修饰变体的多核苷酸来获得。
发明详述
在其最一般的形式中，本发明涉及产生亲本GH8木聚糖酶多肽的变体的方法，所述
方法包括下述步骤：
a)提供编码成熟亲本GH8木聚糖酶多肽的多核苷酸，所述多肽包含第一保守GH8木
聚糖酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1)，
b)将至少一个突变导入编码多核苷酸，由此将至少一个改变在第一保守GH8木聚
糖酶基序N端侧侧翼的20个氨基酸位置中的一个或多个处导入编码的多肽，和
c)将突变的多核苷酸在合适宿主细胞中并在有助于产生变体的条件下进行表达，
其中所述变体具有木聚糖酶活性。
在第一个方面的优选实施方案中，所述成熟亲本GH8木聚糖酶多肽包含氨基酸序
列，所述氨基酸序列与选自SEQ ID NO:3至SEQ ID NO:154和SEQ ID NO:159中任一所示的
GH8木聚糖酶的组中的GH8木聚糖酶的成熟部分至少60％相同；优选所述氨基酸序列与选自
SEQ ID NO:3至SEQ ID NO:154和SEQ ID NO:159中任一所示的GH8木聚糖酶的组中的GH8木
聚糖酶的成熟部分至少65％，更优选至少70％，更优选至少75％，更优选至少80％，更优选
至少85％，甚至更优选至少90％，最优选至少95％，且甚至最优选至少96％，至少97％，至少
98％，或至少99％相同。
在第一个方面的另一个优选实施方案中，所述成熟亲本GH8木聚糖酶包含第二保
守GH8木聚糖酶基序：
[ND][AS]X[RLPQ] (SEQ ID NO:155)。
优选地，所述成熟亲本GH8木聚糖酶包含或组成为SEQ ID NO:3的氨基酸28至433。
在一个优选的实施方案中，所述编码的多肽中的至少一个改变包括在第一保守
GH8木聚糖酶基序N端侧侧翼的20个氨基酸位置中的一个或多个中的一个或多个氨基酸的
取代、缺失和/或插入。
亦优选本发明的第一个方面中所述编码的多肽中的至少一个改变包括在第一保
守GH8木聚糖酶基序N端侧侧翼的15个氨基酸位置中的一个或多个中的一个或多个氨基酸
的插入；优选一个或多个丙氨酸的插入。
在第一个方面的另一个优选实施方案中，所述变体当以充足量在混合之前或混合
过程中加入生面团时，具有至少一种面包或生面团改善特性。
优选地，所述变体当以充足量在混合之前或混合过程中加入生面团时，能够增加
烘烤产品的面包体积或增加烘烤产品表面上切痕的宽度；如当以充足量在混合之前或混合
过程中加入生面团时，所述变体与亲本GH8木聚糖酶相比，如本文中的实施例中所确定(参
见下文)，提供粘性较低的生面团。
具有木聚糖酶活性的多肽
在一个方面，本发明涉及亲本GH8木聚糖酶多肽的分离的变体，所述多肽包含第一
保守GH8木聚糖酶基序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1),
其中所述变体与亲本相比在第一保守GH8基序N端侧侧翼的20个氨基酸位置的一
个或多个中包含至少一个氨基酸改变，且其中所述变体具有木聚糖酶活性。
优选地，所述分离的多肽包含氨基酸序列，所述氨基酸序列与SEQ ID NO:3-154和
159中任一的成熟多肽木聚糖酶具有至少60％，更优选至少65％，更优选至少70％，更优选
至少75％，更优选至少80％，更优选至少85％，甚至更优选至少90％，最优选至少95％，并且
甚至最优选至少96％，至少97％，至少98％，或至少99％的序列同一性程度(在下文中称作
“同源多肽”)。
在一个优选实施方案中，第二个方面的变体选自下述的变体组：
(a)变体多肽，其包含氨基酸序列，所述氨基酸序列与选自SEQ ID NO:3至SEQ ID
NO:154和SEQ ID NO:159中所示的GH8木聚糖酶的组中的GH8木聚糖酶的成熟部分具有至少
60％序列同一性；优选所述氨基酸序列与选自SEQ ID NO:3至SEQ ID NO:154和SEQ ID NO:
159中任一所示的GH8木聚糖酶的组中的GH8木聚糖酶的成熟部分至少65％，更优选至少
70％，更优选至少75％，更优选至少80％，更优选至少85％，甚至更优选至少90％，最优选至
少95％，且甚至最优选至少96％，至少97％，至少98％，或至少99％相同；
(b)变体多肽，其由多核苷酸编码，所述多核苷酸在至少中等-高严格条件下与以
下杂交：(i)SEQ ID NO:2中所示的成熟多肽编码序列，或(ii)(i)的全长互补链；
(c)变体多肽，其由多核苷酸编码，所述多核苷酸包含核苷酸序列，所述核苷酸序
列与SEQ ID NO:2中所示的成熟多肽编码序列具有至少60％序列同一性；和
(d)(a)、(b)或(c)的变体，其包含一个或多个氨基酸的取代、缺失和/或插入。
在另一个优选的实施方案中，所述亲本GH8木聚糖酶多肽包含第二保守GH8木聚糖
酶基序：[ND][AS]X[RLPQ](SEQ ID NO:155)。
还有另一个优选的实施方案涉及第二个方面的变体，其中所述成熟亲本GH8木聚
糖酶多肽包含氨基酸序列，所述氨基酸序列与SEQ ID NO:3中所示的GH8木聚糖酶的成熟部
分至少60％相同；优选地，所述氨基酸序列与SEQID NO:3中所示的GH8木聚糖酶的成熟部分
至少65％，更优选至少70％，更优选至少75％，更优选至少80％，更优选至少85％，甚至更优
选至少90％，最优选至少95％，并且甚至最优选至少96％，至少97％，至少98％，或至少99％
相同。
亦优选第二个方面的变体由多核苷酸编码，所述多核苷酸在至少中等-高严格条
件下与以下杂交：(i)多核苷酸，其包含SEQ ID NO:2的成熟多肽编码序列的核苷酸序列，或
(ii)(i)的全长互补链。
优选地，第二个方面的变体由多核苷酸编码，所述多核苷酸包含核苷酸序列，所述
核苷酸序列与SEQ ID NO:2的成熟多肽编码序列具有至少60％序列同一性；优选至少65％，
更优选至少70％，更优选至少75％，更优选至少80％，更优选至少85％，甚至更优选至少
90％，最优选至少95％，并且甚至最优选至少96％，至少97％，至少98％，或至少99％同一
性。
在一个甚至更优选的实施方案中，第二个方面的变体由多核苷酸编码，所述多核
苷酸包含或组成为SEQ ID NO:2的核苷酸序列，或其编码具有木聚糖酶活性的多肽片段的
亚序列。
甚至更优选的是，所述成熟多肽包含或组成为SEQ ID NO:3的氨基酸28至433，或
其中所述成熟多肽由多核苷酸编码，所述多核苷酸包含或组成为SEQ ID NO:2的核苷酸82
至1299。
另一个优选的实施方案涉及第二个方面的变体，其中所述至少一个氨基酸改变包
含一个或多个氨基酸的取代、缺失和/或插入；优选地，所述至少一个氨基酸改变包含在N端
侧距离第一保守GH8木聚糖酶基序20，19，18，17，16，15，14，13，12，11，10，9，8，7，6，5，4，3，2
和/或1个氨基酸的一个或多个位置处，或在对应于SEQ ID NO:3的成熟多肽中的位置45，
46，47，48，49，50，51，52，53，54，55，56，57，58，59，60，61，62，63和/或64的一个或多个位置
处的一个或多个氨基酸的取代、缺失和/或插入。
在另一个优选的实施方案中，第二个方面的变体中所述至少一个氨基酸改变包含
于第一保守GH8木聚糖酶基序N端侧侧翼的15个氨基酸位置中的一个或多个中，优选包含于
第一保守GH8木聚糖酶基序N端侧侧翼的12个氨基酸位置中的一个或多个中。
亦优选所述至少一个氨基酸改变包含在对应于SEQ ID NO:3的成熟多肽中在N端
侧距离第一保守GH8木聚糖酶基序12个氨基酸的位置的位置处，或在对应于SEQ ID NO:3的
成熟多肽中的位置53的位置处的一个或多个插入。
优选地，所述至少一个氨基酸改变包含在对应于SEQ ID NO:3的成熟多肽中在N端
侧距离第一保守GH8木聚糖酶基序10个氨基酸的位置的位置处，或在对应于SEQ ID NO:3的
成熟多肽中的位置55的位置处的一个或多个插入。
更优选地，所述至少一个氨基酸改变包含在对应于SEQ ID NO:3的成熟多肽中在N
端侧距离第一保守GH8木聚糖酶基序8个氨基酸的位置的位置处，或在对应于SEQ ID NO:3
的成熟多肽中的位置57的位置处的一个或多个插入。
甚至更优选地，所述至少一个氨基酸改变包含在对应于SEQ ID NO:3的成熟多肽
中在N端侧距离第一保守GH8木聚糖酶基序6个氨基酸的位置的位置处，或在对应于SEQ ID
NO:3的成熟多肽中的位置59的位置处的一个或多个插入。
在另一个优选的实施方案中，所述至少一个氨基酸改变包含在对应于SEQ ID NO:
3的成熟多肽中在N端侧距离第一保守GH8木聚糖酶基序4个氨基酸的位置的位置处，或在对
应于SEQ ID NO:3的成熟多肽中的位置61的位置处的一个或多个插入。
还有另一个优选的实施方案涉及第二个方面的变体，其中所述一个或多个插入包
含至少一个丙氨酸残基，至少一个天冬氨酸残基，至少一个丝氨酸残基，至少一个赖氨酸残
基，和/或至少一个苯丙氨酸的插入；优选所述一个或多个插入包含至少一个丙氨酸残基的
插入；优选所述一个或多个插入包含在相同位置或不同位置插入至少一个氨基酸残基和至
少一个额外的但不同的氨基酸残基。
其他优选的实施方案涉及第二个方面的变体，当其以充足量在混合之前或在混合
过程中添加至生面团时具有至少一种面包或生面团改善特性；优选地，当其以充足量在混
合之前或混合过程中添加至生面团时，能够增加烘烤产品的面包体积，或增加烘烤产品表
面上切痕的宽度，并且更优选地，当将其与以相同量添加的亲本GH8木聚糖酶相比时，其提
供了粘性较低的生面团。
在一个优选的方面，所述同源多肽包含氨基酸序列，所述氨基酸序列与SEQ ID
NO:3-154和159中任一的成熟多肽相差十个氨基酸，优选五个氨基酸，更优选四个氨基酸，
甚至更优选三个氨基酸，最优选两个氨基酸，并且甚至最优选一个氨基酸。本发明的多肽优
选包含SEQ ID NO:3-154和159中任一的氨基酸序列，或其等位变体，或它们具有木聚糖酶
活性的片段。在一个优选的方面，所述多肽包含SEQ ID NO:3-154和159中任一的氨基酸序
列。在另一个优选的方面，所述多肽包含SEQ ID NO:3-154和159中任一的成熟多肽。在另一
个优选的方面，所述多肽组成为SEQ ID NO:3-154和159中任一的氨基酸序列。在另一个优
选的方面，所述多肽组成为SEQ ID NO:3-154和159中任一的成熟多肽。
在另一个方面，本发明涉及具有木聚糖酶活性的分离的多肽，所述多肽由多核苷
酸编码，所述多核苷酸在优选非常低严格条件，更优选低严格条件，更优选中等严格条件，
更优选中等-高严格条件，甚至更优选高严格条件，和最优选非常高严格条件下与以下杂
交：(i)SEQ ID NO:2的成熟多肽编码序列，(ii)包含SEQ ID NO:2的成熟多肽编码序列的基
因组DNA序列，或(iii)(i)或(ii)的全长互补链(J.Sambrook,E.F.Fritsch和T.Maniatis,
1989,Molecular Cloning,A Laboratory Manual,第2版,Cold Spring Harbor,New
York)。
SEQ ID NO:2的核苷酸序列或其亚序列，以及SEQ ID NO:3-154和159中任一的氨
基酸序列或其片段，可用于设计核酸探针，以根据本领域内公知的方法从不同属或种的菌
株鉴定和克隆编码具有木聚糖酶活性的多肽的DNA。具体而言，根据标准的Southern印迹方
法，可将这些探针用于与感兴趣的属或种的基因组或cDNA杂交，以鉴定和从其中分离相应
的基因。这些探针可明显短于完整序列，但长度上应为至少14，优选至少25，更优选至少35，
并且最优选至少70个核苷酸。然而，优选所述核酸探针是至少100个核苷酸长度。例如，所述
核酸探针的长度可为至少200个核苷酸，优选至少300个核苷酸，更优选至少400个核苷酸，
或最优选至少500个核苷酸。可使用甚至更长的探针，例如长度为优选至少600个核苷酸，更
优选至少700个核苷酸，甚至更优选至少800个核苷酸，或最优选至少900个核苷酸的核酸探
针。DNA和RNA探针二者均可使用。通常将探针标记以探测相应的基因(例如，用32P、3H、35S、生
物素或抗生物素蛋白(avidin)标记)。这些探针涵盖于本发明中。
因而，可从由这些其它菌株制备的基因组DNA或cDNA文库中筛选DNA，所述DNA与上
述探针杂交并且编码具有木聚糖酶活性的多肽。可以通过琼脂糖或聚丙烯酰胺凝胶电泳，
或通过其它分离技术分离来自这些其它菌株的基因组或其它DNA。可以将来自文库的DNA或
分离的DNA转移至硝化纤维素(nitrocellulose)或其它合适的载体材料并且固定于其上。
为了鉴定与SEQ ID NO:2或其亚序列同源的克隆或DNA，将所述载体材料优选用在
Sounthern印迹中。
就本发明而言，杂交表示核苷酸序列在非常低至非常高的严格条件下与标记的核
酸探针杂交，所述核酸探针对应于SEQ ID NO:2的成熟多肽编码序列；包含SEQ ID NO:2的
成熟多肽编码序列的基因组DNA序列；其全长互补链；或它们的亚序列。可使用例如X射线片
(X-ray film)检测在这些条件下与核酸探针杂交的分子。
在一个优选的方面，核酸探针是SEQ ID NO:2的成熟多肽编码序列。在另一个优选
方面，核酸探针是编码SEQ ID NO:3-154和159中任一的多肽的多核苷酸序列，或其亚序列。
在另一个优选方面，核酸探针是SEQ ID NO:2。
对于长度至少100个核苷酸的长探针，将非常低至非常高的严格条件定义为在42
℃，在5X SSPE、0.3％SDS、200μg/ml已剪切并且变性的鲑精DNA中，并且对于非常低和低严
格性为25％的甲酰胺、对于中等和中等-高严格性为35％的甲酰胺、或对于高和非常高严格
性为50％的甲酰胺，根据标准的Southern印迹法进行预杂交和杂交最佳12至24小时。
对于长度为至少100个核苷酸的长探针，使用2X SSC、0.2％SDS优选在45℃(非常
低严格性)，更优选在50℃(低严格性)，更优选在55℃(中等严格性)，更优选在60℃(中等-
高严格性)，甚至更优选在65℃(高严格性)，并且最优选在70℃(非常高严格性)将载体材料
最终洗涤三次，每次15分钟。
对于长度大约15个核苷酸至大约70个核苷酸的短探针，将严格条件定义为在比根
据Bolton和McCarthy计算法(1962,Proceedings of the National Academy of Sciences
USA 48:1390)得出的Tm低大约5℃至大约10℃，在0.9MNaCl，0.09M Tris-HCl pH 7.6，6mM
EDTA，0.5％NP-40，1×Denhardt溶液，1mM焦磷酸钠(sodium pyrophosphate)，1mM磷酸二氢
钠(sodium monobasic phosphate)，0.1mM ATP和0.2mg每ml的酵母RNA中，根据标准的
Southern印迹步骤进行预杂交、杂交和杂交后洗涤最佳12至24小时。
对于长度大约15个核苷酸至大约70个核苷酸的短探针，将所述载体材料在6×SSC
加0.1％SDS中洗涤一次15分钟，并用6×SSC在比计算的Tm低5℃至10℃的温度洗涤两次，每
次15分钟。
在第三个方面，本发明涉及具有木聚糖酶活性的分离的多肽，所述多肽由多核苷
酸编码，所述多核苷酸包含核苷酸序列或由核苷酸序列组成，所述核苷酸序列与SEQ ID
NO:2的成熟多肽编码序列具有优选至少60％，更优选至少65％，更优选至少70％，更优选至
少75％，更优选至少80％，更优选至少85％，甚至更优选至少90％，最优选至少95％，并且甚
至最优选至少96％，至少97％，至少98％，或至少99％的序列同一性程度，其编码具有木聚
糖酶活性的多肽。参见本文的多核苷酸部分。
在第四个方面，所述具有木聚糖酶活性的分离的多肽包含下述六个氨基酸的长基
序：
[ST]E[GAS]X[GAS][YFW] (SEQ ID NO:1),
其中X是任意氨基酸，且一同列于括号中的氨基酸为可见于单个位置的可选氨基
酸残基，例如[ST]指丝氨酸或苏氨酸可存在于基序中的该位置。在上述基序中，采用公认的
IUPAC单字母氨基酸缩写。
在另一个方面，本发明涉及SEQ ID NO:3-154和159中任一的成熟多肽或其同源序
列的包含取代、缺失和/或插入一个或多个(几个)氨基酸的人工变体。优选地，氨基酸改变
对性质是较不重要的(of a minor nature)，即保守的氨基酸取代或插入，其不显著影响蛋
白质的折叠和/或活性；通常为1至大约30个氨基酸的小缺失；小的氨基或羧基末端延伸，如
氨基末端甲硫氨酸残基；多至大约20-25个残基的小接头肽；或通过改变净电荷或其它功能
来促进纯化的小延伸，如多组氨酸序列(poly histidine tract)、抗原表位(antigenic
epitope)或结合域(binding domain)。
保守取代的实例是在以下组之内：碱性氨基酸组(精氨酸、赖氨酸和组氨酸)、酸性
氨基酸组(谷氨酸和天冬氨酸)、极性氨基酸组(谷氨酰胺和天冬酰胺)、疏水性氨基酸组(亮
氨酸、异亮氨酸和缬氨酸)、芳族氨基酸组(苯丙氨酸、色氨酸和酪氨酸)和小氨基酸组(甘氨
酸、丙氨酸、丝氨酸、苏氨酸和甲硫氨酸)。通常不改变比活性(specific activity)的氨基
酸取代是本领域已知的，并且由例如H.Neurath和R.L.Hill,1979,于The Proteins,
Academic Press,New York中描述。最普遍发生的交换是Ala/Ser、Val/Ile、Asp/Glu、Thr/
Ser、Ala/Gly、Ala/Thr、Ser/Asn、Ala/Val、Ser/Gly、Tyr/Phe、Ala/Pro、Lys/Arg、Asp/Asn、
Leu/Ile、Leu/Val、Ala/Glu和Asp/Gly。
除了20个基本氨基酸，非基本氨基酸(例如4-羟脯氨酸、6-N-甲基赖氨酸、2-氨基
异丁酸、异缬氨酸和α-甲基丝氨酸)可以取代野生型多肽的氨基酸残基。有限数量的非保守
氨基酸、不由遗传密码编码的氨基酸和非天然氨基酸可以取代氨基酸残基。“非天然氨基
酸”在蛋白质合成后已经过修饰，和/或在它们的侧链具有不同于基本氨基酸的化学结构。
非天然氨基酸能够以化学方法合成，并且优选是商业上能够获得的，包括六氢吡啶羧酸
(pipecolic acid)、噻唑烷羧酸(thiazolidine carboxylic acid)、脱氢脯氨酸、3-和4-甲
基脯氨酸，和3,3-二甲基脯氨酸。
可供选择的是，氨基酸改变具有这样的性质以使多肽的物理化学性质改变。例如，
氨基酸改变可改进多肽的热稳定性，改变底物特异性，改变最适pH等。
能够根据本领域已知的方法，例如定位诱变或丙氨酸分区诱变法(Cunningham和
Wells,1989,Science 244:1081-1085)来鉴定亲本多肽中的必需氨基酸。在后一技术中，将
单一丙氨酸突变引入到分子中的每个残基，并且测试所得突变分子的生物活性(即，木聚糖
酶活性)以鉴定对于所述分子的活性关键的氨基酸残基。同样参见Hilton等,1996,
J.Biol.Chem.271:4699-4708。酶的活性部位或其它的生物相互作用也能够通过对结构的
物理分析而测定，如通过以下这些技术：如核磁共振、晶体学、电子衍射或光亲和标记，连同
推定的接触位点氨基酸的突变来测定。参见例如de Vos等,1992,Science 255:306-312；
Smith等,1992,J.Mol.Biol.224:899-904；Wlodaver等,1992,FEBSLett.309:59-64。必需氨
基酸的身份(identity)也能够从与多肽的同一性分析来推断，所述多肽与根据本发明的多
肽相关。
能够使用已知的诱变、重组和/或改组(shuffling)方法，然后是有关的筛选方法，
例如那些由Reidhaar-Olson和Sauer,1988,Science 241:53-57；Bowie和Sauer,1989,
Proc.Natl.Acad.Sci.USA 86:2152-2156；WO 95/17413；或WO95/22625公开的方法来进行
并测试单个或多个氨基酸取代、缺失和/或插入。能够使用的其它方法包括易错PCR、噬菌体
展示(例如，Lowman等,1991,Biochem.30:10832-10837；美国专利No.5,223,409；WO 92/
06204)和区域定向的诱变(Derbyshire等,1986,Gene 46:145；Ner等,1988,DNA 7:127)。
诱变/改组方法能够与高通量、自动化的筛选方法组合以检测由宿主细胞表达的
克隆的、诱变的多肽的活性(Ness等,1999,Nature Biotechnology 17:893-896)。能够从宿
主细胞回收编码活性多肽的诱变的DNA分子，并且使用本领域内标准方法快速测序。这些方
法允许快速测定感兴趣的多肽中单个氨基酸残基的重要性，并且能够应用于未知结构的多
肽。
SEQ ID NO:3-154和159中任一的成熟多肽的氨基酸取代、缺失和/或插入的总数
是10，优选9，更优选8，更优选7，更优选至多6，更优选5，更优选4，甚至更优选3，最优选2，并
且甚至最优选1。
亲本GH8木聚糖酶多肽的来源
本发明的亲本GH8木聚糖酶多肽可以获得自任何属的微生物。就本发明而言，用于
本文与给定的来源有关的术语“获得自”，意思是核苷酸序列编码的多肽由所述来源产生，
或由其中插入了来自所述来源的核苷酸序列的菌株产生。在一个优选的方面，获得自给定
来源的多肽是胞外分泌的。
本发明具有木聚糖酶活性的多肽可以是细菌多肽。例如，所述多肽可以是具有木
聚糖酶活性的革兰氏阳性细菌多肽例如芽孢杆菌属(Bacillus)、链球菌属
(Streptococcus)、链霉菌属(Streptomyces)、葡萄球菌属(Staphylococcus)、肠球菌属
(Enterococcus)、乳杆菌属(Lactobacillus)、乳球菌属(Lactococcus)、梭菌属
(Clostridium)、地芽孢杆菌属(Geobacillus)或海洋芽孢杆菌属(Oceanobacillus)多肽；
或具有木聚糖酶活性的革兰氏阴性细菌多肽，如大肠杆菌(E.coli)、假单胞菌属
(Pseudomonas)、沙门氏菌属(Salmonella)、弯曲杆菌属(Campylobacter)、螺杆菌属
(Helicobacter)、黄杆菌属(Flavobacterium)、梭杆菌属(Fusobacterium)、泥杆菌属
(Ilyobacter)、奈瑟氏菌属(Neisseria)或脲原体属(Ureaplasma)多肽。
在一个优选方面，所述多肽是具有木聚糖酶活性的嗜碱芽孢杆菌(Bacillus
alkalophilus)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、短芽孢杆菌(Bacillus
brevis)、环状芽孢杆菌(Bacillus circulans)、克劳氏芽孢杆菌(Bacillus clausii)、凝
结芽孢杆菌(Bacillus coagulans)、坚强芽孢杆菌(Bacillus firmus)、灿烂芽孢杆菌
(Bacillus lautus)、迟缓芽孢杆菌(Bacillus lentus)、地衣芽孢杆菌(Bacillus
licheniformis)、巨大芽孢杆菌(Bacillus megaterium)、短小芽孢杆菌(Bacillus
pumilus)、嗜热脂肪芽孢杆菌(Bacillus stearothermophilus)、枯草芽孢杆菌(Bacillus
subtilis)或苏云金芽孢杆菌(Bacillus thuringiensis)多肽。
在另一个优选的方面，所述多肽是具有木聚糖酶活性的似马链球菌
(Streptococcus equisimilis)、酿脓链球菌(Streptococcus pyogenes)、乳房链球菌
(Streptococcus uberis)或马链球菌兽瘟亚种(Streptococcus equi
subsp.Zooepidemicus)多肽。
在另一个优选的方面，所述多肽是具有木聚糖酶活性的不产色链霉菌
(Streptomyces achromogenes)、除虫链霉菌(Streptomyces avermitilis)、天蓝链霉菌
(Streptomyces coelicolor)、灰色链霉菌(Streptomyces griseus)或浅青紫链霉菌
(Streptomyces lividans)多肽。
本发明具有木聚糖酶活性的多肽也可以是真菌多肽，并且更优选具有木聚糖酶活
性的酵母多肽如假丝酵母属(Candida)、克鲁维酵母属(Kluyveromyces)、毕赤酵母属
(Pichia)、酵母属(Saccharomyces)、裂殖酵母属(Schizosaccharomyces)或西洋蓍霉属
(Yarrowia)多肽；或更优选具有木聚糖酶活性的丝状真菌多肽如枝顶孢霉属
(Acremonium)、伞菌属(Agaricus)、链格孢属(Alternaria)、曲霉属(Aspergillus)、短梗霉
属(Aureobasidium)、Botryospaeria、拟蜡菌属(Ceriporiopsis)、毛喙壳属
(Chaetomidium)、金孢子菌属(Chrysosporium)、Claviceps、Cochliobolus、鬼伞属
(Coprinopsis)、Coptotermes、棒囊壳属(Corynascus)、隐丛赤壳菌属(Cryphonectria)、隐
球菌属(Cryptococcus)、色二孢属(Diplodia)、黑耳属(Exidia)、Filibasidium、镰孢属
(Fusarium)、赤霉属(Gibberella)、全鞭毛虫属(Holomastigotoides)、腐质霉属
(Humicola)、耙齿菌属(Irpex)、蘑菇属(Lentinula)、Leptospaeria、梨孢菌属
(Magnaporthe)、Melanocarpus、多孔菌属(Meripilus)、毛霉属(Mucor)、毁丝霉属
(Myceliophthora)、新考玛脂霉属(Neocallimastix)、脉孢菌属(Neurospora)、拟青霉属
(Paecilomyces)、青霉属(Penicillium)、平革菌属(Phanerochaete)、瘤胃壶菌属
(Piromyces)、Poitrasia、假黑盘菌属(Pseudoplectania)、Pseudotrichonympha、根毛霉属
(Rhizomucor)、裂褶菌属(Schizophyllum)、柱顶孢属(Scytalidium)、踝节菌属
(Talaromyces)、嗜热子囊菌属(Thermoascus)、梭孢壳属(Thielavia)、弯颈霉属
(Tolypocladium)、木霉属(Trichoderma)、长毛盘菌属(Trichophaea)、轮枝孢属
(Verticillium)、包脚菇属(Volvariella)或炭角菌属(Xylaria)多肽。
在一个优选的方面，所述多肽是具有木聚糖酶活性的卡尔酵母(Saccharomyces
carlsbergensis)、酿酒酵母(Saccharomyces cerevisiae)、糖化酵母(Saccharomyces
diastaticus)、道格拉氏酵母(Saccharomyces douglasii)、克鲁弗酵母(Saccharomyces
kluyveri)、诺地酵母(Saccharomyces norbensis)或卵形酵母(Saccharomyces
oviformis)多肽。
在另一个优选的方面，所述多肽是具有木聚糖酶活性的解纤维枝顶孢霉
(Acremonium cellulolyticus)、棘孢曲霉(Aspergillus aculeatus)、泡盛曲霉
(Aspergillus awamori)、烟曲霉(Aspergillus fumigatus)、臭曲霉(Aspergillus
foetidus)、日本曲霉(Aspergillus japonicus)、构巢曲霉(Aspergillus nidulans)、黑曲
霉(Aspergillus niger)、米曲霉(Aspergillus oryzae)、嗜角质金孢子菌(Chrysosporium
keratinophilum)、Chrysosporium lucknowense、热带金孢子菌(Chrysosporium
tropicum)、Chrysosporium merdarium、Chrysosporium inops、毡金孢子菌
(Chrysosporium pannicola)、Chrysosporium queenslandicum、Chrysosporium zonatum、
杆孢状镰孢(Fusarium bactridioides)、禾谷镰孢(Fusarium cerealis)、库威镰孢
(Fusarium crookwellense)、大刀镰孢(Fusarium culmorum)、禾本科镰孢(Fusarium
graminearum)、禾赤镰孢(Fusarium graminum)、异孢镰孢(Fusarium heterosporum)、合欢
木镰孢(Fusarium negundi)、尖镰孢(Fusarium oxysporum)、多枝镰孢(Fusarium
reticulatum)、粉红镰孢(Fusarium roseum)、接骨木镰孢(Fusarium sambucinum)、肤色镰
孢(Fusarium sarcochroum)、拟分枝孢镰孢(Fusarium sporotrichioides)、硫色镰孢
(Fusarium sulphureum)、圆镰孢(Fusarium torulosum)、拟丝孢镰孢(Fusarium
trichothecioides)、镶片镰孢(Fusarium venenatum)、灰腐质霉(Humicola grisea)、特异
腐质霉(Humicola insolens)、疏棉状腐质霉(Humicola lanuginosa)、白耙齿菌(Irpex
lacteus)、米黑毛霉(Mucor miehei)、嗜热毁丝霉(Myceliophthora thermophila)、粗糙脉
孢菌(Neurospora crassa)、绳状青霉(Penicillium funiculosum)、产紫青霉
(Penicillium purpurogenum)、黄孢平革菌(Phanerochaete chrysosporium)、无色梭孢壳
(Thielavia achromatica)、Thielavia albomyces、Thielavia albopilosa、澳洲梭孢壳
(Thielavia australeinsis)、Thielavia fimeti、小孢梭孢壳(Thielavia microspora)、
卵孢梭孢壳(Thielavia ovispora)、Thielavia peruviana、瘤孢梭孢壳(Thielavia
spededonium)、毛梭孢壳(Thielavia setosa)、Thielavia subthermophila、土生梭孢霉
(Thielavia terrestris)、哈茨木霉(Trichoderma harzianum)、康宁木霉(Trichoderma
koningii)、长枝木霉(Trichoderma longibrachiatum)、里氏木霉(Trichoderma reesei)
或绿色木霉(Trichoderma viride)多肽。
可理解的是对于前述的种，本发明包含完全和不完全两个阶段(both the
perfect and imperfect states)，和其它分类学的等同物(equivalent)，例如无性型
(anamorph)，而无论它们已知的种名。本领域熟练技术人员将容易地识别适合的等同物的
同一性。
这些种的菌株在许多培养物保藏中心对于公众能够容易地取得，所述保藏中心诸
如美国典型培养物保藏中心(the American Type Culture Collection)(ATCC)、德意志微
生物和细胞培养物保藏中心(Deutsche Sammlung von Mikroorganismen und
Zellkulturen GmbH)(DSM)、真菌菌种保藏中心(Centraalbureau Voor
Schimmelcultures)(CBS)和农业研究机构专利培养物保藏中心北区研究中心
(Agricultural Research Service Patent Culture Collection,Northern Regional
Research Center)(NRRL)。
此外，可以使用上述的探针从其它来源，包括从自然界(例如，土壤、堆肥、水等)分
离的微生物鉴定和获得这些多肽。用于从天然生境(habitat)分离微生物的技术是本领域
内公知的。随后可通过相似地筛选这种微生物的基因组或cDNA文库来获得所述多核苷酸。
一旦用所述探针检测到编码多肽的多核苷酸，就能够使用本领域普通技术人员熟知的技术
将所述多核苷酸分离或克隆(参见，例如，Sambrook等,1989,见上文)。
一个属于GH8家族、具有(或预期具有)木聚糖酶活性的多肽的非穷尽列表提供于
表1，参见下文。
本发明的多肽还包括融合多肽或可切割的融合多肽，其中将另外的多肽融合到所
述多肽或其片段的N末端或C末端。通过将编码另一个多肽的核苷酸序列(或其部分)融合于
本发明的核苷酸序列(或其部分)来产生融合的多肽。产生融合多肽的技术是本领域已知
的，并包括连接编码多肽的编码序列以使它们在阅读框中，并且使所述融合多肽的表达在
相同启动子和终止子的控制下。
融合多肽还可以包括切割位点。一旦分泌了融合多肽，就切割所述位点，从融合蛋
白释放具有木聚糖酶活性的多肽。切割位点的实例包括，但不限于，编码二肽Lys-Arg的
Kex2位点(Martin等,2003,J.Ind.Microbiol.Biotechnol.3:568-76；Svetina等，2000,
J.Biotechnol.76:245-251；Rasmussen-Wilson等，1997,Appl.Environ.Microbiol.63:
3488-3493；Ward等,1995,Biotechnology 13:498-503；和Contreras等,1991,
Biotechnology 9:378-381)；Ile-(Glu或Asp)-Gly-Arg位点，其在精氨酸残基后通过因子
Xa蛋白酶切割(Eaton等,1986,Biochem.25:505-512)；Asp-Asp-Asp-Asp-Lys位点，其在赖
氨酸后通过肠激酶切割(Collins-Racie等,1995,Biotechnology 13:982-987)；His-Tyr-
Glu位点或His-Tyr-Asp位点，其通过Genenase I切割(Carter等,1989,Proteins:
Structure,Function,and Genetics 6:240-248)；Leu-Val-Pro-Arg-Gly-Ser位点，其在
Arg后通过凝血酶切割(Stevens,2003,Drug Discovery World 4:35-48)；Glu-Asn-Leu-
Tyr-Phe-Gln-Gly位点，其在Gln后通过TEV蛋白酶切割(Stevens,2003，见上)；和Leu-Glu-
Val-Leu-Phe-Gln-Gly-Pro位点，其在Gln后通过遗传工程形式的人鼻病毒3C蛋白酶切割
(Stevens,2003，见上)。
多核苷酸
本发明还涉及分离的多核苷酸，其包含或组成为编码本发明的变体GH8木聚糖酶
多肽的核苷酸序列。
在一个优选的方面，所述核苷酸序列包含或组成为SEQ ID NO:2，其中根据本发明
的方法导入了一个或多个突变。在另一个优选的方面，所述核苷酸序列包含或组成为SEQ
ID NO:2的成熟多肽编码序列，其中根据本发明的方法导入了一个或多个突变。本发明亦涵
盖了核苷酸序列，其编码由亲本GH8木聚糖酶制备的变体多肽，所述亲本GH8木聚糖酶包含
或组成为SEQ ID NO:3的氨基酸序列或其成熟多肽，所述核苷酸序列与SEQ ID NO:2或其成
熟多肽编码序列由于遗传密码的简并性而存在差异。本发明还涉及SEQ ID NO:2的亚序列，
其中根据本发明的方法导入了一个或多个突变，所述亚序列编码由亲本GH8木聚糖酶制备
的变体多肽的片段，所述亲本GH8木聚糖酶包含或组成为具有木聚糖酶活性的SEQ ID NO:3
的氨基酸序列。
本发明还涉及突变多核苷酸，所述突变多核苷酸包含或组成为至少一个突变的
SEQ ID NO:2的成熟多肽编码序列，其中所述突变核苷酸序列编码由亲本GH8木聚糖酶制备
的成熟变体多肽，所述亲本GH8木聚糖酶包含或组成为SEQ ID NO:3的氨基酸序列。
用于分离或克隆编码多肽的多核苷酸的技术是本领域内已知的，包括从基因组
DNA分离，从cDNA制备，或其组合。可通过例如使用熟知的聚合酶链式反应(PCR)或表达文库
的抗体筛选来检测具有共有结构特性的克隆DNA片段，从而实现从这种基因组DNA克隆本发
明的多核苷酸。参见，例如，Innis等,1990,PCR:A Guide to Methods and Application,
Academic Press,New York。可以使用其它核酸扩增方法，如连接酶链式反应(LCR)、连接活
化转录(ligated activated transcription；LAT)和基于核苷酸序列的扩增(NASBA)。
本发明还涉及分离的多核苷酸，其包含或组成为核苷酸序列，所述核苷酸序列与
SEQ ID NO:2的成熟多肽编码序列具有优选至少60％，更优选至少65％，更优选至少70％，
更优选至少75％，更优选至少80％，更优选至少85％，甚至更优选至少90％，最优选至少
95％，并且甚至最优选至少96％，至少97％，至少98％，或至少99％的序列同一性程度，其编
码本发明的具有木聚糖酶活性的变体多肽。
修饰编码本发明多肽的核苷酸序列对于合成与所述多肽基本上相似的多肽可能
为必需的。术语与所述多肽“基本上相似”指多肽的非天然存在的形式。这些多肽可能以一
些工程改造的方式而不同于从其天然来源分离的多肽，例如，比活性、热稳定性、最适pH等
方面不同的人工变体。可以在作为SEQ ID NO:2的成熟多肽编码序列存在的核苷酸序列，例
如其亚序列的基础上，和/或通过引入如下核苷酸取代来构建变体序列：所述取代不产生由
核苷酸序列编码的多肽的另外的氨基酸序列，但是符合意欲产生酶的宿主生物体的密码子
选择；或者所述取代可产生不同的氨基酸序列。关于核苷酸取代的概述，参见，例如，Ford
等,1991,Protein Expression and Purification 2:95-107。
对于本领域技术人员显而易见的是，这些取代能够在对于分子功能重要的区域之
外进行，并且仍然产生活性多肽。对于由本发明的分离的多核苷酸编码的多肽活性关键的
并且因此优选不进行取代的氨基酸残基，可以根据本领域公知的方法，例如定位诱变或丙
氨酸分区诱变法(参见，例如，Cunningham和Wells,1989,见上文)来鉴定。在后一技术中，将
突变引入到分子中的每个荷正电的残基处，并且测试所得突变分子的木聚糖酶活性，以鉴
定对于所述分子的活性关键的氨基酸残基。底物-酶相互作用的位点也能够通过分析三维
结构测定，通过如核磁共振分析、晶体学或光亲和标记这样的技术来测定(参见，例如，de
Vos等,1992,见上文；Smith等,1992,见上文；Wlodaver等,1992,见上文)。
本发明还涉及编码本发明多肽的分离的多核苷酸，所述分离的多核苷酸在非常低
严格条件下，优选低严格条件，更优选中等严格条件，更优选中等-高严格条件，甚至更优选
高严格条件，并且最优选非常高的严格条件下，与以下序列杂交：(i)SEQ ID NO:2的成熟多
肽编码序列，(ii)包含SEQ ID NO:2的成熟多肽编码序列的基因组DNA序列，或(iii)(i)或
(ii)的全长互补链；或它们的等位变体和亚序列(Sambrook等,1989,见上文)，如本文所定
义的。
本发明还涉及分离的多核苷酸，所述分离的多核苷酸通过以下方法获得：(a)在非
常低、低、中等、中等-高、高或非常高的严格条件下，将DNA群体与以下杂交：(i)SEQ ID NO:
2的成熟多肽编码序列，(ii)包含SEQ ID NO:2的成熟多肽编码序列的基因组DNA序列，或
(iii)(i)或(ii)的全长互补链；和(b)分离杂交的多核苷酸，其编码具有木聚糖酶活性的多
肽。
核酸构建体
本发明还涉及包含本发明的分离的多核苷酸的核酸构建体，所述分离的多核苷酸
与一个或多个(几个)调控序列可操作地连接，所述调控序列在合适的宿主细胞中在与该调
控序列相容的条件下指导编码序列的表达。
可以用许多方式操作编码本发明多肽的分离的多核苷酸以提供多肽的表达。依赖
于表达载体，在将多核苷酸的序列插入载体之前对其进行操作可能是理想的或必需的。使
用重组DNA方法修饰多核苷酸序列的技术是本领域熟知的。
调控序列可以是适当的启动子序列，其是由用于表达编码本发明多肽的多核苷酸
的宿主细胞识别的核苷酸序列。启动子序列含有介导多肽的表达的转录调控序列。启动子
可以是在所选的宿主细胞中显示转录活性的任何核苷酸序列，包括突变的、截短的和杂合
的启动子，并且可以从编码与宿主细胞同源或异源的胞外或胞内多肽的基因获得。
用于指导本发明的核酸构建体转录，特别是在细菌宿主细胞中转录的合适启动子
的实例是从下述获得的启动子：大肠杆菌lac操纵子、天蓝链霉菌(Streptomyces
coelicolor)琼脂糖酶基因(dagA)、枯草芽孢杆菌果聚糖蔗糖酶基因(sacB)、地衣芽孢杆菌
α-淀粉酶基因(amyL)、嗜热脂肪芽孢杆菌产麦芽淀粉酶基因(amyM)、解淀粉芽孢杆菌α-淀
粉酶基因(amyQ)、地衣芽孢杆菌青霉素酶基因(penP)、枯草芽孢杆菌xylA和xylB基因和原
核β-内酰胺酶基因(Villa-Kamaroff等,1978,Proceedings of the National Academy of
Sciences USA 75:3727-3731)，以及tac启动子(DeBoer等,1983,Proceedings of the
National Academy of Sciences USA 80:21-25)。另外的启动子在"Useful proteins
from recombinant bacteria"于Scientific American,1980,242:74-94中；和在Sambrook
等,1989,见上文中描述。
用于指导本发明的核酸构建体在丝状真菌宿主细胞中转录的合适启动子的实例
是从下列酶的基因获得的启动子：米曲霉TAKA淀粉酶、曼赫根毛霉(Rhizomucor miehei)天
冬氨酸蛋白酶、黑曲霉中性α-淀粉酶、黑曲霉酸稳定性α-淀粉酶、黑曲霉或泡盛曲霉葡糖淀
粉酶(glaA)、曼赫根毛霉脂肪酶、米曲霉碱性蛋白酶、米曲霉丙糖磷酸异构酶、构巢曲霉乙
酰胺酶、镶片镰孢淀粉葡糖苷酶(WO 00/56900)、镶片镰孢Daria(WO 00/56900)、镶片镰孢
Quinn(WO 00/56900)、尖镰孢胰蛋白酶样蛋白酶(WO 96/00787)、里氏木霉β-葡糖苷酶、里
氏木霉纤维二糖水解酶I、里氏木霉纤维二糖水解酶II、里氏木霉内切葡聚糖酶I、里氏木霉
内切葡聚糖酶II、里氏木霉内切葡聚糖酶III、里氏木霉内切葡聚糖酶IV、里氏木霉内切葡
聚糖酶V、里氏木霉木聚糖酶I、里氏木霉木聚糖酶II、里氏木霉β-木糖苷酶，以及NA2-tpi启
动子(一种修饰的启动子，来自在黑曲霉中编码中性α-淀粉酶的基因，其中未翻译的前导序
列由在构巢曲霉中编码丙糖磷酸异构酶的基因的未翻译的前导序列所替代)；和它们的突
变的、截短的和杂合的启动子。
在酵母宿主中，有用的启动子从如下酶的基因获得：酿酒酵母烯醇化酶(ENO-1)、
酿酒酵母半乳糖激酶(GAL1)、酿酒酵母醇脱氢酶/甘油醛-3-磷酸脱氢酶(ADH1,ADH2/GAP)、
酿酒酵母丙糖磷酸异构酶(TPI)、酿酒酵母金属硫蛋白(CUP1)和酿酒酵母3-磷酸甘油酸激
酶。对于酵母宿主细胞其它有用的启动子由Romanos等,1992,Yeast 8:423-488描述。
调控序列也可以是合适的转录终止子序列，是由宿主细胞识别以终止转录的序
列。所述终止子序列与编码所述多肽的核苷酸序列的3’末端可操作地连接。可以将在所选
宿主细胞中有功能的任何终止子用在本发明中。
对于丝状真菌宿主细胞优选的终止子从如下酶的基因获得：米曲霉TAKA淀粉酶、
黑曲霉葡糖淀粉酶、构巢曲霉邻氨基苯甲酸合酶、黑曲霉α-葡糖苷酶和尖镰孢胰蛋白酶样
蛋白酶。
对于酵母宿主细胞优选的终止子从如下酶的基因获得：酿酒酵母烯醇化酶、酿酒
酵母细胞色素C(CYC1)和酿酒酵母甘油醛-3-磷酸脱氢酶。对于酵母宿主细胞其它有用的终
止子由Romanos等,1992,见上文描述。
调控序列还可以是合适的前导序列，其为对于宿主细胞的翻译重要的mRNA非翻译
区。前导序列可操作地连接于编码多肽的核苷酸序列的5’-末端。可以将在所选宿主细胞中
有功能的任何前导序列用在本发明中。
对于丝状真菌宿主细胞优选的前导序列从如下酶的基因获得：米曲霉TAKA淀粉酶
和构巢曲霉丙糖磷酸异构酶。
对于酵母宿主细胞合适的前导序列从如下酶的基因获得：酿酒酵母烯醇化酶
(ENO-1)、酿酒酵母3-磷酸甘油酸激酶、酿酒酵母α因子和酿酒酵母醇脱氢酶/甘油醛-3-磷
酸脱氢酶(ADH2/GAP)。
调控序列也可以是聚腺苷酸化序列，其是与核苷酸序列的3’末端可操作地连接的
序列，并且在转录时，宿主细胞将其识别为将聚腺苷残基添加至转录的mRNA的信号。可以将
在所选宿主细胞中有功能的任何聚腺苷酸化序列在本发明中使用。
对于丝状真菌宿主细胞优选的聚腺苷酸化序列从如下酶的基因获得：米曲霉TAKA
淀粉酶、黑曲霉葡糖淀粉酶、构巢曲霉邻氨基苯甲酸合酶、尖镰孢胰蛋白酶样蛋白酶和黑曲
霉α-葡糖苷酶。
对于酵母宿主细胞有用的聚腺苷酸化序列由Guo和Sherman,1995,Molecular
Cellular Biology 15:5983-5990描述。
调控序列还可以是信号肽编码序列，其编码与多肽的氨基末端相连的信号肽，并
且指导编码的多肽进入细胞分泌途径。核苷酸序列的编码序列5’端可固有地包含信号肽编
码序列，其与编码分泌多肽的编码序列片段一起天然地连接在翻译阅读框中。可供选择的
是，编码序列5’端可含有对于所述编码序列异源的信号肽编码序列。异源信号肽编码序列
在编码序列不天然地含有信号肽编码序列时可为必需的。或者，外源信号肽编码序列可以
简单地取代天然信号肽编码序列以增强多肽的分泌。然而，指导表达的多肽进入所选宿主
细胞的分泌途径(即，分泌至培养基中)的任何信号肽编码序列可在本发明中使用。
对于细菌宿主细胞有效的信号肽编码序列是从如下酶的基因获得的信号肽编码
序列：芽孢杆菌属NCIB 11837产麦芽糖淀粉酶、嗜热脂肪芽孢杆菌α-淀粉酶、地衣芽孢杆菌
枯草杆菌蛋白酶(subtilisin)、地衣芽孢杆菌β-内酰胺酶、嗜热脂肪芽孢杆菌中性蛋白酶
(nprT,nprS,nprM)和枯草芽孢杆菌prsA。另外的信号肽由Simonen和Palva,1993,
Microbiological Reviews 57:109-137描述。
对于丝状真菌宿主细胞有效的信号肽编码序列是从如下酶的基因获得的信号肽
编码序列：米曲霉TAKA淀粉酶、黑曲霉中性淀粉酶、黑曲霉葡糖淀粉酶、曼赫根毛霉天冬氨
酸蛋白酶、特异腐质霉纤维素酶、特异腐质霉内切葡聚糖酶V和疏棉状腐质霉脂肪酶。
对于酵母宿主细胞有用的信号肽从酿酒酵母α因子和酿酒酵母转化酶的基因获
得。其它有用的信号肽编码序列由Romanos等,1992,见上文描述。
在一个优选的方面，信号肽包含或组成为SEQ ID NO:3的氨基酸-27至-1或SEQ ID
NO:4中的-28至-1。在另一个优选方面，信号肽编码序列包含或组成为SEQ ID NO:2的核苷
酸1至81。
调控序列还可以是前肽编码序列，其编码位于多肽氨基末端的前肽。所得多肽称
为酶原(proenzyme)或前多肽(propolypeptide)(或在某些情况下称为酶原(zymogen))。前
多肽通常是无活性的并且能够通过前肽的催化或自催化切割从前多肽转化为成熟活性多
肽。可以从枯草芽孢杆菌碱性蛋白酶(aprE)、枯草芽孢杆菌中性蛋白酶(nprT)、酿酒酵母α
因子、曼赫根毛霉天冬氨酸蛋白酶和嗜热毁丝霉漆酶(WO 95/33836)的基因获得前肽编码
序列。
当信号肽和前肽序列二者均出现在多肽的氨基末端时，将前肽序列置于紧接着
(next to)多肽氨基末端，并且将信号肽序列置于紧接着前肽序列的氨基末端。
同样理想的是添加调节序列，其允许相对于宿主细胞的生长来调节多肽的表达。
调节系统的实例是引起基因表达响应化学或物理刺激物，包括调节化合物的存在而开启或
关闭的那些系统。原核系统中的调节系统包括lac、tac和trp操纵基因系统。在酵母中，可以
使用ADH2系统或GAL1系统。在丝状真菌中，可以使用TAKA α-淀粉酶启动子、黑曲霉葡糖淀
粉酶启动子和米曲霉葡糖淀粉酶启动子作为调节序列。调节序列的其它实例是那些允许基
因扩增的序列。在真核系统中，这些调节序列包括在氨甲蝶呤(methotrexate)存在下扩增
的二氢叶酸还原酶基因，和以重金属(with heavy metal)扩增的金属硫蛋白基因。在这些
情况下，编码多肽的核苷酸序列将与调节序列可操作地连接。
表达载体
本发明还涉及重组表达载体，所述重组表达载体包含本发明的多核苷酸、启动子
和转录和翻译终止信号。本文所述的多种核酸和调控序列可以结合在一起以产生重组表达
载体，所述表达载体可以包括一个或多个(几个)方便的限制位点以允许在这些位点插入或
取代编码多肽的核苷酸序列。可供选择的是，可以通过在适当的用于表达的载体中插入包
含所述序列的核苷酸序列或核酸构建体来表达本发明的多核苷酸序列。在制备表达载体的
过程中，将编码序列置于载体中，从而将该编码序列与适当的表达调控序列可操作地连接。
重组表达载体可以是任何载体(例如，质粒或病毒)，其能够方便地进行重组DNA步
骤，并且能够产生核苷酸序列的表达。载体的选择将通常依赖于载体与将引入该载体的宿
主细胞的相容性。载体可以是线状或闭合环状质粒。
载体可以是自主复制载体，即，作为染色体外实体(entity)存在的载体，其复制独
立于染色体复制，例如，质粒、染色体外元件、微型染色体(minichromosome)或人工染色体。
载体可以含有任何用于确保自复制的手段(means)。或者，载体可以是一种当被引入宿主细
胞中时，整合到基因组中并且与整合了该载体的染色体一起复制的载体。此外，可以使用单
独的载体或质粒或两个或更多个载体或质粒，其共同含有待引入宿主细胞基因组的完整
DNA(total DNA)，或可以使用转座子(transposon)。
本发明的载体优选地含有一个或多个(几个)选择性标记，其允许简单选择经转
化、转染、转导等的细胞。选择性标记是基因，其产物提供杀生物剂或病毒抗性、对重金属的
抗性、对营养缺陷型的原养性(prototrophy to auxotrophs)等。
细菌选择性标记的实例是来自枯草芽孢杆菌或地衣芽孢杆菌的dal基因，或赋予
抗生素抗性的标记，所述抗生素抗性例如氨苄青霉素、卡那霉素、氯霉素或四环素抗性。对
于酵母宿主细胞合适的标记是ADE2、HIS3、LEU2、LYS2、MET3、TRP1和URA3。用于丝状真菌宿
主细胞的选择性标记包括但不限于amdS(乙酰胺酶)、argB(鸟氨酸氨甲酰基转移酶)、bar
(草铵膦(phosphinothricin)乙酰转移酶)、hph(潮霉素磷酸转移酶)、niaD(硝酸还原酶)
(nitrate reductase)、pyrG(乳清酸核苷-5’-磷酸脱羧酶)(orotidine-5’-phosphate
decarboxylase)、sC(硫酸腺苷酰转移酶)和trpC(邻氨基苯甲酸合酶(anthranilate
synthase))以及它们的等同物。优选用在曲霉属细胞中的是构巢曲霉或米曲霉的amdS和
pyrG基因和吸水链霉菌(Streptomyces hygroscopicus)的bar基因。
本发明的载体优选含有元件，其允许载体整合入宿主细胞基因组或载体在细胞中
独立于基因组的自主复制。
为了整合入宿主细胞基因组，载体可依赖编码多肽的多核苷酸的序列或用于通过
同源或非同源重组整合入基因组的任何其它载体元件。或者，载体可以含有额外的核苷酸
序列，用于指导通过同源重组整合入宿主细胞基因组染色体中的精确位置。为了增加在精
确位置整合的可能性，整合元件应优选含有足够数量的核酸，如100至10,000碱基对，优选
400至10,000碱基对，并且最优选800至10,000碱基对，其与相应的目标序列具有高度序列
同一性以增强同源重组的概率。整合元件可以是任何序列，其与宿主细胞基因组中的目标
序列同源。此外，整合元件可以是非编码或编码的核苷酸序列。另一方面，可以将载体通过
非同源重组整合到宿主细胞的基因组中。
为了自主复制，载体可以进一步包含复制起点，其使载体能够在所述的宿主细胞
中自主地复制。复制起点可以是介导自主复制的任何质粒复制子(replicator)，其在细胞
中发挥功能。术语“复制起点”或“质粒复制子”在本文定义为能够使质粒或载体体内复制的
核苷酸序列。
细菌复制起点的实例是允许在大肠杆菌中复制的质粒pBR322、pUC19、pACYC177和
pACYC184的复制起点，和允许在芽孢杆菌属中复制的质粒pUB110、pE194、pTA1060和pAMβ1
的复制起点。
用于酵母宿主细胞中的复制起点的实例是2微米复制起点，ARS1,ARS4,ARS1和
CEN3的组合，和ARS4和CEN6的组合。
在丝状真菌细胞中有用的复制起点的实例是AMA1和ANS1(Gems等,1991,Gene 98:
61-67；Cullen等,1987,Nucleic Acids Research 15:9163-9175；WO 00/24883)。分离AMA1
基因和构建包含该基因的质粒或载体能够根据公开于WO 00/24883中的方法完成。
可以将多于一个拷贝的本发明的多核苷酸插入宿主细胞以增加基因产物的产生。
多核苷酸拷贝数的增加可通过如下方法获得：将至少一个额外拷贝的序列整合入宿主细胞
基因组，或将可扩增的选择性标记基因包括于多核苷酸，其中可通过在合适的选择剂
(selectable agent)存在下培养细胞来选择含有选择性标记基因的扩增拷贝，且由此含有
多核苷酸的额外拷贝的细胞。
用于连接上述元件以构建本发明的重组表达载体的方法是本领域技术人员熟知
的(参见，例如，Sambrook等,1989,见上文)。
宿主细胞
本发明还涉及重组宿主细胞，其包含可操作地连接的本发明的分离的多核苷酸和
一个或多个(几个)调控序列，所述调控序列指导具有木聚糖酶活性的多肽的产生。将包含
本发明多核苷酸的构建体或载体导入宿主细胞，使构建体或载体如前所述作为染色体整合
体或者作为自复制的染色体外载体维持。术语“宿主细胞”包括亲本细胞的任何后代，其由
于复制过程中发生的突变而不同于亲本细胞。宿主细胞的选择将在很大程度上依赖于编码
多肽的基因及其来源。
宿主细胞可以是在本发明的多肽的重组产生中有用的任何细胞，例如，原核或真
核细胞。
原核宿主细胞可以是任何革兰氏阳性细菌或革兰氏阴性细菌。革兰氏阳性细菌包
括但不限于，芽孢杆菌属、链球菌属、链霉菌属、葡萄球菌属、肠球菌属、乳酸菌属、乳球菌
属、梭菌属、地芽孢杆菌属和海洋芽孢杆菌属。革兰氏阴性细菌包括但不限于，大肠杆菌、假
单胞菌属、沙门氏菌属、弯曲杆菌属、螺杆菌属、黄杆菌属、梭杆菌属、泥杆菌属、奈瑟氏菌属
和脲原体属。
细菌宿主细胞可以是任何芽孢杆菌属细胞。在本发明的实施中有用的芽孢杆菌属
细胞包括但不限于，嗜碱芽孢杆菌、解淀粉芽孢杆菌、短芽孢杆菌、环状芽孢杆菌、克劳氏芽
孢杆菌、凝结芽孢杆菌、坚强芽孢杆菌、灿烂芽孢杆菌、迟缓芽孢杆菌、地衣芽孢杆菌、巨大
芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌和苏云金芽孢杆菌细胞。
在一个优选的方面，细菌宿主细胞是解淀粉芽孢杆菌、迟缓芽孢杆菌、地衣芽孢杆
菌、嗜热脂肪芽孢杆菌或枯草芽孢杆菌细胞。在一个更优选的方面，细菌宿主细胞是解淀粉
芽孢杆菌细胞。在另一个更优选的方面，细菌宿主细胞是克劳氏芽孢杆菌细胞。在另一个更
优选的方面，细菌宿主细胞是地衣芽孢杆菌细胞。在另一个更优选的方面，细菌宿主细胞是
枯草芽孢杆菌细胞。
细菌宿主细胞还可以是任何链球菌属细胞。在本发明的实施中有用的链球菌属细
胞包括但不限于，似马链球菌、酿脓链球菌、乳房链球菌和马链球菌兽瘟亚种细胞。
在一个优选的方面，细菌宿主细胞是似马链球菌细胞。在另一个优选的方面，细菌
宿主细胞是酿脓链球菌细胞。在另一个优选的方面，细菌宿主细胞是乳房链球菌细胞。在另
一个优选的方面，细菌宿主细胞是马链球菌兽瘟亚种细胞。
细菌宿主细胞还可以是任何链霉菌属细胞。在本发明的实施中有用的链霉菌属细
胞包括但不限于，不产色链霉菌、除虫链霉菌、天蓝链霉菌、灰色链霉菌和浅青紫链霉菌细
胞。
在一个优选的方面，细菌宿主细胞是不产色链霉菌细胞。在另一个优选的方面，细
菌宿主细胞是除虫链霉菌细胞。在另一个优选的方面，细菌宿主细胞是天蓝链霉菌细胞。在
另一个优选的方面，细菌宿主细胞是灰色链霉菌细胞。在另一个优选的方面，细菌宿主细胞
是浅青紫链霉菌细胞。
可通过如下方法实现将DNA引入到芽孢杆菌属细胞：例如原生质体转化(参见，例
如，Chang和Cohen,1979,Molecular General Genetics 168:111-115)，使用感受态细胞
(参见，例如，Young和Spizizen,1961,Journal of Bacteriology 81:823-829或Dubnau和
Davidoff-Abelson,1971,Journal of Molecular Biology 56:209-221)，电穿孔(参见，例
如，Shigekawa和Dower,1988,Biotechniques 6:742-751)或接合(参见，例如，Koehler和
Thorne,1987,Journal of Bacteriology 169:5771-5278)。可通过如下方法实现将DNA引
入到大肠杆菌细胞：例如原生质体转化(参见，例如，Hanahan,1983,J.Mol.Biol.166:557-
580)或电穿孔(参见，例如，Dower等,1988,Nucleic Acids Res.16:6127-6145)。可通过如
下方法实现将DNA引入到链霉菌属细胞：例如原生质体转化和电穿孔(参见，例如，Gong等,
2004,Folia Microbiol.(Praha)49:399-405)，接合(参见，例如，Mazodier等,1989,
J.Bacteriol.171:3583-3585)，或转导(参见，例如，Burke等,2001,
Proc.Natl.Acad.Sci.USA 98:6289-6294)。可通过如下方法实现将DNA引入到假单胞菌属
细胞：例如电穿孔(参见，例如，Choi等,2006,J.Microbiol.Methods 64:391-397)或接合
(参见，例如，Pinedo和Smets,2005,Appl.Environ.Microbiol.71:51-57)。可通过如下方法
实现将DNA引入到链球菌属细胞：例如天然感受态(natural competence)(参见，例如，
Perry和Kuramitsu,1981,Infect.Immun.32:1295-1297)，原生质体转化(参见，例如，Catt
和Jollick,1991,Microbios.68:189-207)，电穿孔(参见，例如，Buckley等,1999,
Appl.Environ.Microbiol.65:3800-3804)或接合(参见，例如，Clewell,1981,
Microbiol.Rev.45:409-436)。然而，可以使用本领域已知的将DNA引入宿主细胞的任何方
法。
宿主细胞还可以是真核生物，如哺乳动物、昆虫、植物或真菌细胞。
在一个优选的方面，宿主细胞是真菌细胞。“真菌”用在本文包括以下门：子囊菌门
(Ascomycota)、担子菌门(Basidiomycota)、壶菌门(Chytridiomycota)和接合菌门
(Zygomycota)(如由Hawksworth等,于Ainsworth and Bisby’s Dictionary of The
Fungi,第8版,1995,CAB International,University Press,Cambridge,UK中所定义)以及
卵菌门(Oomycota)(如Hawksworth等,1995,见上,171页中所引用)，和所有有丝分裂孢子真
菌(mitosporic fungi)(Hawksworth等,1995,见上文)。
在一个更优选的方面，真菌宿主细胞是酵母细胞。“酵母”用在本文包括产子囊酵
母(ascosporogenous yeast)(内孢霉目(Endomycetales))、产担子酵母
(basidiosporogenous yeast)和属于半知菌类(Fungi Imperfecti)(芽孢纲
(Blastomycetes))的酵母。由于酵母的分类在未来可能改变，就本发明而言，将酵母定义为
如Biology and Activities of Yeast(Skinner,F.A.,Passmore,S.M.,和Davenport,R.R.
编,Soc.App.Bacteriol.Symposium Series No.9,1980)中所述。
在一个甚至更加优选的方面，酵母宿主细胞是假丝酵母属、汉逊酵母属
(Hansenula)、克鲁维酵母属、毕赤酵母属、酵母属、裂殖酵母属或西洋蓍霉属细胞。
在一个最优选的方面，酵母宿主细胞是卡尔酵母、酿酒酵母、糖化酵母、道格拉氏
酵母、克鲁弗酵母、诺地酵母或卵形酵母细胞。在另一个最优选的方面，酵母宿主细胞是乳
酸克鲁维酵母(Kluyveromyces lactis)细胞。在另一个最优选的方面，酵母宿主细胞是解
脂西洋蓍霉(Yarrowia lipolytica)细胞。
在另一个更优选的方面，真菌宿主细胞是丝状真菌细胞。“丝状真菌”包括真菌门
(Eumycota)和卵菌门的亚门(如由Hawksworth等,1995,见上文，所定义)的所有丝状形式。
丝状真菌通常的特征在于由壳多糖(chitin)、纤维素、葡聚糖、壳聚糖(chitosan)、甘露聚
糖和其它复杂多糖组成的菌丝体壁。通过菌丝延伸进行营养生长，而碳分解代谢是专性需
氧的。相反，酵母如酿酒酵母的营养生长通过单细胞菌体的出芽生殖(budding)进行，而碳
分解代谢可以是发酵的。
在一个甚至更优选的方面，丝状真菌宿主细胞是枝顶孢霉属、曲霉属、短梗霉属、
烟管霉属(Bjerkandera)、拟蜡菌属、金孢子菌属、鬼伞属(Coprinus)、革盖菌属
(Coriolus)、隐球菌属、Filibasidium、镰孢属、腐质霉属、梨孢菌属、毛霉属、毁丝霉属、新
考玛脂霉属、脉孢菌属、拟青霉属、青霉属、平革菌属(Phanerochaete)、射脉菌属
(Phlebia)、瘤胃壶菌属、侧耳属(Pleurotus)、裂褶菌属、踝节菌属、嗜热子囊菌属、梭孢壳
属、弯颈霉属、栓菌属(Trametes)或木霉属细胞。
在最优选的方面，丝状真菌宿主细胞是泡盛曲霉、烟曲霉、臭曲霉、日本曲霉、构巢
曲霉、黑曲霉或米曲霉细胞。在另一个最优选方面，丝状真菌宿主细胞是杆孢状镰孢、禾谷
镰孢、库威镰孢、大刀镰孢、禾本科镰孢、禾赤镰孢、异孢镰孢、合欢木镰孢、尖镰孢、多枝镰
孢、粉红镰孢、接骨木镰孢、肤色镰孢、拟分枝孢镰孢、硫色镰孢、圆镰孢、拟丝孢镰孢或镶片
镰孢细胞。在另一个最优选的方面，丝状真菌宿主细胞是黑刺烟管菌(Bjerkandera
adusta)、干拟蜡菌(Ceriporiopsis aneirina)、干拟蜡菌、Ceriporiopsis caregiea、
Ceriporiopsis gilvescens、Ceriporiopsis pannocinta、Ceriporiopsis rivulosa、
Ceriporiopsis subrufa、虫拟蜡菌(Ceriporiopsis subvermispora)、嗜角质金孢子菌、
Chrysosporium lucknowense、热带金孢子菌、Chrysosporium merdarium、Chrysosporium
inops、毡金孢子菌、Chrysosporium queenslandicum、Chrysosporium zonatum、灰盖鬼伞
(Coprinus cinereus)、毛革盖菌(Coriolus hirsutus)、特异腐质霉、疏棉状腐质霉、米黑
毛霉、嗜热毁丝霉、粗糙脉孢菌、产紫青霉、黄孢平革菌(Phanerochaete chrysosporium)、
辐射射脉菌(Phlebia radiata)、刺芹侧耳(Pleurotus eryngii)、土生梭孢霉、长绒毛栓菌
(Trametes villosa)、变色栓菌(Trametes versicolor)、哈茨木霉、康宁木霉、长枝木霉、
里氏木霉或绿色木霉细胞。
可以将真菌细胞通过涉及原生质体形成、原生质体转化和细胞壁重建的方法以本
身公知的方式转化。用于转化曲霉属和木霉属宿主细胞的合适方法在EP 238 023和Yelton
等,1984,Proceedings of the National Academy of Sciences USA 81:1470-1474中描
述。用于转化镰孢属菌种的合适方法由Malardier等,1989,Gene 78:147-156和WO 96/
00787描述。可以使用由如下文献描述的方法转化酵母：Becker和Guarente,于Abelson,
J.N.和Simon,M.I.编,Guide to Yeast Genetics and Molecular Biology,Methods in
Enzymology,Volume 194,pp 182-187,Academic Press,Inc.,New York；Ito等,1983,
Journal of Bacteriology 153:163；和Hinnen等,1978,Proceedings of the National
Academy of Sciences USA 75:1920。
产生方法
本发明还涉及用于产生本发明变体多肽的方法，其包括：(a)在有助于产生多肽的
条件下培养细胞，所述细胞产生所述多肽；和(b)回收所述多肽。在一个优选的方面，所述细
胞是芽孢杆菌属的细胞。在一个更优选的方面，所述细胞是地衣芽孢杆菌。
本发明还涉及用于产生本发明的变体多肽的方法，其包括：(a)如本文所述，在有
助于产生多肽的条件下培养重组宿主细胞；和(b)回收所述多肽。
本发明还涉及用于产生本发明的变体多肽的方法，包括：(a)在有助于产生多肽的
条件下培养重组宿主细胞，其中所述宿主细胞包含突变核苷酸序列，其在SEQ ID NO:2的成
熟多肽编码序列中具有至少一个突变，其中所述突变核苷酸序列编码本发明的SEQ ID NO:
3-154和159的成熟多肽中任一的变体，和(b)回收所述多肽。
在本发明的产生方法中，使用本领域熟知的方法在适合于产生所述多肽的营养培
养基中培养细胞。例如，可以通过在合适培养基中和允许表达和/或分离所述多肽的条件下
进行的摇瓶培养，和实验室或工业发酵罐中的小规模或大规模发酵(包括连续、分批、补料
分批或固态发酵)来培养细胞。使用本领域已知的方法在合适的营养培养基中进行培养，所
述营养培养基包含碳源和氮源和无机盐。合适的培养基能够从商业供应商获得或可以根据
公开的组成制备(例如，在美国典型培养物保藏中心的目录中)。如果多肽分泌到营养培养
基中，该多肽能够从所述培养基中直接回收。如果多肽不分泌到培养基中，其能够从细胞裂
解物(lysate)回收。
可以使用本领域已知的对于所述多肽是特异性的方法来检测多肽。这些检测方法
可包括特异性抗体的使用、酶产物的形成或酶底物的消失。例如，酶试验(enzyme assay)可
用于测定如本文所述的多肽的活性。
所得多肽可以使用本领域已知的方法回收。例如，多肽可以通过常规方法从营养
培养基中回收，所述常规方法包括但不限于离心、过滤、提取、喷雾干燥、蒸发或沉淀。
本发明的多肽可以通过多种本领域已知的方法纯化以获得基本上纯的多肽，所述
方法包括但不限于层析(例如，离子交换、亲和、疏水、层析聚焦和大小排阻)、电泳方法(例
如，制备型(preparative)等电聚焦)、差示溶解度(例如，硫酸铵沉淀)、SDS-PAGE或提取(参
见，例如，Protein Purification,J.-C.Janson和Lars Ryden,editors,VCH Publishers,
New York,1989)。
组合物
本发明还涉及包含本发明的变体多肽的组合物。优选地，所述组合物富含这种多
肽。术语“富含”表示所述组合物的变体GH8木聚糖酶活性，例如，以至少1.1的富集因数
(enrichment factor)增加。
所述组合物可以包含本发明的多肽作为主要酶成分，例如，单成分组合物。或者，
所述组合物可以包含多种酶活性，如氨肽酶、淀粉酶、糖酶、羧肽酶、过氧化氢酶、纤维素酶、
几丁质酶、角质酶、环糊精糖基转移酶、脱氧核糖核酸酶、酯酶、α-半乳糖苷酶、β-半乳糖苷
酶、葡糖淀粉酶、α-葡糖苷酶、β-葡糖苷酶、卤素过氧化物酶、转化酶、漆酶、脂肪酶、甘露糖
苷酶、氧化酶、果胶分解酶、肽谷氨酰胺酶(peptidoglutaminase)、过氧化物酶、肌醇六磷酸
酶、多酚氧化酶、蛋白水解酶、核糖核酸酶、转谷氨酰胺酶或木聚糖酶。额外的酶可以通过例
如属于以下属或种的微生物产生：曲霉属，优选棘孢曲霉、泡盛曲霉、烟曲霉、臭曲霉、日本
曲霉、构巢曲霉、黑曲霉或米曲霉；镰孢属，优选杆孢状镰孢、禾谷镰孢、库威镰孢、大刀镰
孢、禾本科镰孢、禾赤镰孢、异孢镰孢、合欢木镰孢、尖镰孢、多枝镰孢、粉红镰孢、接骨木镰
孢、肤色镰孢、硫色镰孢、圆镰孢、拟丝孢镰孢或镶片镰孢；腐质霉属，优选特异腐质霉或疏
棉状腐质霉；或木霉属，优选哈茨木霉、康宁木霉、长枝木霉、里氏木霉或绿色木霉。
可以依照本领域内已知的方法制备多肽组合物，并且可以是液体或干组合物的形
式。例如，所述多肽组合物可以是颗粒(granulate)或微粒(microgranulate)的形式。可以
依照本领域内已知方法将包括于所述组合物中的多肽稳定化。
下面给出的是本发明多肽组合物的优选的用途的实例。本发明的多肽组合物的剂
量和使用该组合物的其它条件可以基于本领域内已知的方法确定。
用途
本发明亦涉及使用具有GH8木聚糖酶活性的变体多肽，或其组合物的方法，包括烘
烤应用。
在一个方面，本发明涉及用于制备烘烤产品的方法，所述方法包括将第二个方面
的变体多肽或包含所述变体多肽的面包改善组合物添加至所述烘烤产品的生面团的步骤。
在一个优选实施方案中，所述变体多肽或面包改善组合物在生面团的混合过程中
添加。
在另一个优选的实施方案中，所述面包改善组合物进一步包含一种或多种选自下
列的面包改善剂：酶、乳化剂、氧化剂、奶粉、脂肪、糖、氨基酸、盐、蛋白(谷蛋白，纤维素结合
位点)或其混合物，优选所述酶选自下列：α-淀粉酶、β-淀粉酶、产麦芽糖淀粉酶、其他木聚
糖酶、蛋白酶、葡糖氧化酶、氧化还原酶、葡聚糖酶、纤维素酶、转谷氨酰胺酶、异构酶、脂肪
酶、磷脂酶、果胶酶或其混合物。
优选所述变体多肽作为细胞提取物、无细胞提取物或纯化蛋白存在。
在另一个优选的实施方案中，将所述变体多肽与干粉或颗粒，特别是无尘颗粒形
式，或液体形式的其他成分混合，优选与一种或多种稳定剂如多元醇、糖、有机酸或糖醇混
合。
烘烤产品
本发明的工艺或GH8木聚糖酶变体可用于从生面团制备的任何种类的烘烤产品，
其可具有柔软或松脆的性质，白色、浅色或深色类型的。实例为面包(特别是白面包、全麦面
包或黑麦面包)，通常为枕形面包或小白面包，法国面包型面包(French baguette-type
bread)，扁平面包(flat bread)，口袋饼/皮塔饼(pita bread)，未经发酵的玉米饼
(tortilla)、蛋糕、薄烤饼、饼干、曲奇饼、大馅饼皮(pie crust)、脆面包(crisp bread)、馒
头、比萨饼等的形式。
预混物
本发明进一步涉及预混物，其包含面粉以及本发明的GH8变体木聚糖酶多肽。所述
预混物可含有其他生面团改善和/或面包改善添加剂，例如任何如上所提及的添加剂，包括
酶。
酶制备物
本发明提供了包含本发明的GH8变体木聚糖酶的酶制备物，以在本发明的工艺中
用作烘烤添加剂。所述酶制备物优选为颗粒或团聚粉末的形式。其优选具有狭窄的粒径分
布，其中超过95％(按重量计)的颗粒在25至500μm的范围。
颗粒和团聚粉末可通过常规方法制备，例如将木聚糖酶在流化床制粒机中喷洒于
载体上。所述载体可由具有合适粒径的颗粒核心组成。所述载体可为可溶的或不可溶的，例
如盐(如NaCl或硫酸钠)，糖(如蔗糖或乳糖)，糖醇(如甘露醇)，淀粉，大米，粗碾玉米(corn
grits)或大豆。
通过以下实施例进一步对本发明进行描述，但不应将其理解为对本发明范围的限
制。
实施例
本文描述和要求保护的本发明并不局限于本文公开的具体方面的范围内，因为这
些方面旨在作为本发明几个方面的说明。旨在将任何等同的方面包含于本发明的范围内。
实际上，从前面的说明中，除本文所显示和描述的之外，本发明的多种修改对于本领域的技
术人员来说是显而易见的。这些修改也旨在落入所附的权利要求的范围内。在冲突的情况
下，将以包括定义部分的本公开为准。
作为缓冲液和底物使用的化学品为至少试剂级别的商品。
实施例1：亲本GH8木聚糖酶
在下表1中提供了多种潜在的亲本GH8木聚糖酶的非限定性和非穷尽性列表。所列
的每个GH8木聚糖酶包含SEQ ID NO:1的保守GH8基序，对于每个序列，其氨基酸位置标示于
最右栏。序列标识符(SEQ ID NO)标示于表最左栏，生物名称和UniProtKB登录号分别示于
第二和第三栏。当适用时，标示了预测或显示为信号肽部分的氨基酸，以及预测或显示包含
于成熟GH8多肽的氨基酸。信号肽中的氨基酸在所附序列表中标示为’-’，但在下表中从全
长多肽的起始密码子开始编号。并非所有列出的多肽均分泌。一些列出的多肽亦可包含前
原序列，其会被切割去以形成成熟多肽，但在大部分情况下作为成熟多肽一部分显示——
这可通过本领域的标准实践如成熟多肽的N端氨基酸测序来确定。
表1：潜在的亲本GH8木聚糖酶；G8基序1标示于成熟序列编号中，其中包括信号作
为序列表(其中标为负数)中的特征，其他情况下从信号起点开始标示。
实施例2：通过定点诱变构建变体
根据本发明构建了芽孢杆菌属菌种KK-1GH8木聚糖酶(SEQ ID NO:3或4)在保守
GH8基序N端侧侧翼的环中包含特定插入/缺失/取代的定点变体。这些变体通过传统的DNA
片段克隆(Sambrook等,Molecular Cloning:A Laboratory Manual,2nd Ed.,Cold Spring
Harbor,1989)使用PCR以及适当地设计的诱变寡核苷酸(其在所得序列中导入所需的突变)
来制备。
诱变寡核苷酸对应于所需突变位点侧翼的DNA序列合成，其由限定所述插入/缺
失/取代的DNA碱基对分隔。以此方式，构建并产生了列于下表2的变体。
作为实例，如下所示设计了用于构建变体LBei411(其在成熟多肽的位置53具有单
个丙氨酸氨基酸插入(*53aA))的诱变引物：
LBei411f(SEQ ID NO:156)：5’-gcttctacaacagacgctgacaaccttggcaacggctattac
LBei411r(SEQ ID NO:157)：5’-gtaatagccgttgccaaggttgtcagcgtctgttgtagaagc
为了纯化本发明的GH8木聚糖酶变体，将包含本发明变体的突变DNA转化入感受态
枯草芽孢杆菌菌株并使用标准实验方案发酵(PS-1培养基，3-4日，30℃)。
表2：芽孢杆菌属菌种KK-1GH8木聚糖酶(SEQ ID NO:3或SEQ ID NO:4)的环变体，
其中对于每个变体列出了氨基酸突变。氨基酸位置号53-62涉及SEQ ID NO:3或SEQ ID NO:
4的成熟多肽。氨基酸缺失显示为缺失的氨基酸、其位置号和星号，例如“D53*”指从位置53
缺失天冬氨酸残基(D)。插入实现显示为星号继以位置号，表示已经存在于该位置的氨基酸
的小写“a”，最后是插入的氨基酸，例如“*53aA”指在位置53的天冬氨酸(D)之后在多肽中插
入一个丙氨酸(A)残基。氨基酸取代显示为氨基酸、其位置号和替代或取代第一个提及的氨
基酸的新氨基酸，例如“N55A”指位置55的天冬酰胺(N)残基用丙氨酸(A)残基替代。
实施例3：直接生面团过程
配方
步骤
1.称取(scale)成分，添加面粉、酵母、盐、糖、抗坏血酸和酶；
2.将酶以0.02至0.2mg蛋白酶/kg面粉施用；阴性对照除外(无酶)；
3.调整水的温度以达到在混合、称取并将水添加至混合碗之后27℃的生面团温
度；
4.混合：使用Diosna螺旋混合器以78rpm进行3分钟和以138rpm进行6分钟；
5.从混合碗取生面团，并确定生面团温度，确定生面团参数(在混合之后进行生面
团评价)，并用轻便烘炉(baker)模制生面团；
6.在塑料覆盖下给予生面团20分钟的第一发酵(floor-time)，并进行第二生面团
评价(在第一发酵之后的生面团评价)；
7.称取1500g生面团(30个小白面包)和6x 350g(面包)；
8.对模制的小白面包和面包生面团覆盖塑料进行10分钟第二发酵(bench-time)；
9.将用于小白面包的生面团制成-34cm的圆盘，并置于小白面包制作平板(roll
maker plate)上，并用揉圆机(rounder)制成小白面包：
a.将小白面包转移至用硅胶覆盖的烤板(baking sheet)上；
b.将用于面包的生面团在压面机上成型，并转移至平底锅，将平底锅置于烤板中；
10.将面包和小白面包在32℃，86％RH醒发；
a.对于小白面包的醒发时间为45分钟和70分钟(过度醒发)；
b.对于面包的醒发时间为55分钟和80分钟(过度醒发)；
11.将面包和小白面包在230℃用蒸汽烘烤：
a.将小白面包烘烤22分钟(在12分钟之后开放气闸(damper)以从烤箱释放蒸汽)；
b.将面包烘烤35分钟(在25分钟之后开放气闸以从烤箱释放蒸汽)；
12.在烘烤之后从平底锅取出面包，并置于烤板上；
13.允许面包和小白面包冷却；
14.对所述面包和小白面包进行评价。
实施例4：Chorleywood烘烤步骤(CBP)
配方
步骤
1.称取(scale)成分，添加面粉、水、膏质酵母、盐、大豆粉、抗坏血酸和酶；
2.将酶以0.02至0.2mg蛋白酶/kg面粉施用；阴性对照除外(无酶)；
3.将所有成分置于Pentagon K5High Speed混合器中；
4.将生面团以425rpm的速度混合至11w/kg生面团的能量输入(以0.6巴大气压进
行30％的混合时间，并在18hg真空进行70％的混合时间)；
5.评价生面团参数(在混合之后进行生面团评价)；
6.在塑料覆盖下将生面团静置(resting)(第一次)5分钟；
7.称取4x 600g生面团；
8.在塑料覆盖下将生面团静置(第二次)10分钟；
9.对生面团进行模制，并在压面机中成型，并转移至带盖的平底锅，并置于烤板
上；
10.将生面团在40℃，80RH在Termaks醒发箱中进行醒发：
a.对于平底锅面包，醒发时间为55分钟；
b.对于3切面包(3-cut-bread)，醒发时间为45分钟；
11.在220℃在Reed Trap烤箱中不用蒸汽烘烤面包
12.对所述面包进行评价。
实施例5：生面团和面包评价参数
粘性：生面团粘性是生面团粘附于人手或其他表面的程度。这是由有经验的烤面
包师进行的感官评价，其中给予不用酶的对照生面团以5的值，并将其他生面团相对于对照
以0至10的尺度评判，其中0为几乎无粘性，而10为具有非常大粘性。
柔软性：生面团柔软性是生面团压缩的容易程度。这是由有经验的烤面包师进行
的感官评价，其中给予不用酶的对照生面团以5的值，并将其他生面团相对于对照以0至10
的尺度评判，其中0为较不柔软，而10为更加柔软。
可延展性：生面团可延展性是生面团可延展而不撕裂的程度。这是由有经验的烤
面包师进行的感官评价，其中给予不用酶的对照生面团以5的值，并将其他生面团相对于对
照以0至10的尺度评判，其中0为低/短的可延展性，而10为高/长的可延展性。
弹性：生面团弹性是生面团抵抗拉伸以及当移除受力时回复其初始大小和形状的
能力。这是由有经验的烤面包师进行的感官评价，其中给予不用酶的对照生面团以5的值，
并将其他生面团相对于对照以0至10的尺度评判，其中0为低/弱的可延展性，而10为高/强
的可延展性。
可加工性：可加工性是生面团在机器中加工之后保持其初始表面而无损伤的趋势
的程度。这是由有经验的烤面包师进行的感官评价，其中给予不用酶的对照生面团以5的
值，并将其他生面团相对于对照以0至10的尺度评判，其中0为低可加工性，而10为高可加工
性。
体积：烘烤的小白面包和面包的体积是通过菜籽顶替(rape seed displacement)
来确定的。比体积指数(specific volume index)如下计算：
比体积＝用酶的面包的比体积(ml/g)/不用酶的面包的比体积(ml/g)
将两个对照生面团的平均比体积设为100％。从具有添加酶的生面团制备的面包
或小白面包的比体积为两次样品的平均值。
实施例6：评价GH8木聚糖酶亲本的性能
在大多数情况下，确定如上所述的生面团和面包参数涉及有经验的烤面包师进行
的感官评价，但即使如此，取决于测试进行的日期和哪个烤面包师进行的测试等，很有可能
会存在一些天然差异。
对芽孢杆菌属菌种KK-1(SEQ ID NO:3或4)的成熟亲本GH8木聚糖酶在数日内由至
少两名烤面包师在十二个实验中，在直接生面团步骤中用来自Meneba的Kolibri面粉使用
0.05mg/kg的酶剂量进行测试。在第一发酵之后评价生面团的粘性、柔软性、可延展性、弹性
和可加工性。亦在正常醒发和长醒发之后确定所得面包的比体积。结果示于下表3：
表3：实施例6的生面团评价的结果：
实施例7：使用GH8木聚糖酶变体制备的生面团的粘性
在第一发酵之后评价使用GH8木聚糖酶变体在用来自Meneba的Kolibri面粉以
0.05mg/kg的酶剂量进行的直接生面团步骤中制备的生面团的粘性。对于某些提供显著较
低粘性的生面团的更令人感兴趣的变体，亦对0.1mg/kg、4倍过剂量(4xOD)和10倍过量剂量
(10xOD)的更高酶剂量进行了第一发酵之后的评价。结果示于下表4。
本发明的GH8变体(一些甚至在非常高酶剂量下)与亲本GH8木聚糖酶相比存在提
供粘性较低的生面团的趋势。
表4：实施例7的生面团评价；由烤面包师给出的粘性评分的结果
实施例8：用GH8木聚糖酶变体制备的面包的体积增加
从用GH8木聚糖酶变体在直接生面团步骤中用来自Meneba的Kolibri面粉以
0.05mg/kg的酶剂量使用正常和长醒发制备的生面团烘烤面包。确定面包的比体积。
对于一些更令人感兴趣的变体，亦使用以正常和长醒发用0.1mg/kg、4倍过量剂量
(4xOD)和10倍过量剂量(10xOD)的更高酶剂量制成的生面团进行了烘烤测试。结果示于下
表5：
表5：实施例8的比面包体积的结果：
实施例9：使用GH8木聚糖酶变体制备的生面团的柔软性等
在第一发酵之后评价使用GH8木聚糖酶变体在直接生面团步骤中用来自Meneba的
Kolibri面粉以0.05mg/kg的酶剂量制备的生面团的柔软性、可延展性、弹性和可加工性。结
果示于下表6：
表6：0.05mg/kg的酶剂量
如前所述，对于一些提供显著较低粘性面团的更令人感兴趣的变体，亦对于
0.1mg/kg、4倍过量剂量(4xOD)和10倍过量剂量(10xOD)的更高酶剂量进行了第一发酵之后
的评价。这些对于柔软性、可延展性、弹性和可加工性的结果示于下表7-9。
表7：0.1mg/kg的酶剂量
变体
突变
柔软性
可延展性
弹性
可加工性
LBei411
*53aA
6
5
5
4
LBei413
*55aA
6
4
6
5
LBei413
*55aA
6
5
5
4
LBei417
*59aA
6
4
6
5
LBei417
*59aA
6
5
5
4
MDTo253
*60aA
7
6
4
4
MDTo254
*62aA
6
5
5
4
MDTo255
*53aS
7
5
5
5
MDTo256
*53aD
7
6
4
2
MDTo257
*53aK
6
6
4
4
MDTo258
*53aF
6
5
5
4
MDTo259
*53aL
7
6
4
4
MDTo260
*55aS
7
6
4
4
MDTo262
*55aK
8
7
4
4
MDTo263
*55aF
6
6
5
5
MDTo264
*55aL
6
6
4
5
MDTo265
*59aS
6
5
5
5
MDTo266
*59aD
6
6
4
5
MDTo267
*59aK
6
6
4
5
MDTo268
*59aF
6
6
4
5
MDTo269
*59aL
6
6
4
5
MDTo323
*53aA,*55aL
7
6
4
4
MDTo324
*55aA,*59aD
7
6
4
4
MDTo325
*55aL,*59aA
8
6
4
5
MDTo326
*55aL,*59aS
8
6
4
5
MDTo327
*55aL,*59aD
8
6
4
5
MDTo328
*53aA,*59aS
7
6
4
4
MDTo329
*53aA,*59aD
7
6
4
5
MDTo330
*53aA,*55aL,*59aA
7
6
4
5
MDTo331
*53aA,*55aA,*59aS
8
6
4
5
MDTo332
*53aA,*55aA,*59aD
8
6
4
5
MDTo333
*53aA,*55aA,*59aF
7
7
4
5
MDTo334
*53aA,*55aL,*59aS
8
7
4
5
MDTo335
*53aA,*55aL,*59aD
7
6
4
4
MDTo336
*53aA,*55aL,*59aF
7
6
4
4
表8：4倍过量剂量(4xOD)的酶剂量
变体
突变
柔软性
可延展性
弹性
可加工性
LBei411
*53aA
6
5
5
4
LBei413
*55aA
6
4
6
5
LBei413
*55aA
6
5
5
4
LBei417
*59aA
6
4
6
5
LBei417
*59aA
6
5
5
4
MDTo253
*60aA
7
6
4
4
MDTo254
*62aA
6
5
5
4
MDTo255
*53aS
7
5
5
5
MDTo256
*53aD
7
6
4
2
MDTo257
*53aK
6
6
4
4
MDTo258
*53aF
6
5
5
4
MDTo259
*53aL
7
6
4
4
MDTo260
*55aS
7
6
4
4
MDTo262
*55aK
8
7
4
4
MDTo263
*55aF
6
6
5
5
MDTo264
*55aL
6
6
4
5
MDTo265
*59aS
6
5
5
5
MDTo266
*59aD
6
6
4
5
MDTo267
*59aK
6
6
4
5
MDTo268
*59aF
6
6
4
5
MDTo269
*59aL
6
6
4
5
MDTo323
*53aA,*55aL
8
6
4
4
MDTo327
*55aL,*59aD
8
6
4
4
表9：10倍过量剂量的酶剂量(10xOD)：
实施例10：在使用低品质面粉的直接生面团步骤中的GH8变体
亦在直接生面团步骤中但用低品质面粉测试了十个GH8变体。酶剂量为0.05mg/kg
或0.1mg/kg。
从两种酶剂量使用正常和长醒发时间烘烤面包，并确定比体积。结果总结于下表
10和11。
表10：在直接生面团步骤中使用具有较低谷蛋白含量的面粉在0.05mg/kg和
0.1mg/kg的酶剂量在第一发酵之后的生面团粘性和分别在正常和长醒发之后的比面包体
积
表11：在直接生面团步骤中使用具有较低谷蛋白含量的面粉以0.05mg/kg和
0.1mg/kg的酶剂量制成的生面团的柔软性(Soft)、可延展性(Ext)、弹性(Elas)和可加工性
(Mach)
实施例11：在Chorleywood烘烤步骤中的GH8变体
亦在Chorleywood烘烤步骤(Chorleywood Baking Procedure；CBP)中测试了使用
0.05和0.1mg/kg的酶剂量的本发明十一个GH8变体在混合之后的生面团粘性和在正常醒发
之后的烘烤的面包的比体积增加。结果示于表12。
表12：在使用0.05mg/kg(0.05)或0.1mg/kg(0.01)酶剂量的Chorleywood烘烤步骤
中混合之后的生面团粘性
表13：在Chorleywood步骤中用0.05mg/kg和0.1mg/kg的酶剂量制备的生面团的柔
软性(Soft)，可延展性(Ext)，弹性(Elas)和可加工性(Mach)。
序列表
<110> 诺维信公司(Novozymes A/S)
<120> 产生GH8木聚糖酶变体的方法
<130> 11635-CN-PCD
<160> 159
<170> PatentIn version 3.5
<210> 1
<211> 6
<212> PRT
<213> 人工序列
<220>
<223> GH8木聚糖酶保守基序1
<220>
<221> NON_CONS
<222> (1)..(1)
<223> 保守基序中位置1的氨基酸为丝氨酸(S)或苏氨酸(T)
<220>
<221> NON_CONS
<222> (3)..(3)
<223> 位置3的氨基酸为甘氨酸(G), 丙氨酸(A)或丝氨酸(S)
<220>
<221> NON_CONS
<222> (4)..(4)
<223> 位置4的氨基酸为任意氨基酸
<220>
<221> NON_CONS
<222> (5)..(5)
<223> 位置5的氨基酸为甘氨酸(G), 丙氨酸(A)或丝氨酸(S)
<220>
<221> NON_CONS
<222> (6)..(6)
<223> 位置6的氨基酸为酪氨酸(Y), 苯丙氨酸(F)或色氨酸(W)
<400> 1
Xaa Glu Xaa Xaa Xaa Xaa
1 5
<210> 2
<211> 1302
<212> DNA
<213> 人工序列
<220>
<223> 对于芽孢杆菌属表达优化的合成编码DNA序列
<220>
<221> CDS
<222> (1)..(1299)
<220>
<221> 信号肽
<222> (1)..(81)
<220>
<221> 成熟肽
<222> (82)..(1299)
<400> 2
atg aag aaa ccg ttg ggg aaa att gtc gca agc acc gca cta ctc att 48
Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile
-25 -20 -15
tct gtt gct ttt agt tca tcg atc gca tcg gct gct gta cat tca aag 96
Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Val His Ser Lys
-10 -5 -1 1 5
act cca gac atc ctt ggc aca act ggc aag aac aac ctt aac cag gct 144
Thr Pro Asp Ile Leu Gly Thr Thr Gly Lys Asn Asn Leu Asn Gln Ala
10 15 20
tac aag aag tat ttc gac aca aag ggc gac ggc aaa ggt ggc tct ctt 192
Tyr Lys Lys Tyr Phe Asp Thr Lys Gly Asp Gly Lys Gly Gly Ser Leu
25 30 35
ttt cac tat atg aag gac ggc tct gct tac atc gct tct aca aca gac 240
Phe His Tyr Met Lys Asp Gly Ser Ala Tyr Ile Ala Ser Thr Thr Asp
40 45 50
gac aac ctt ggc aac ggc tat tac tct gta aag act gag ggc atg tca 288
Asp Asn Leu Gly Asn Gly Tyr Tyr Ser Val Lys Thr Glu Gly Met Ser
55 60 65
tac ggc atg atg atc acg ctt caa atg aac gac gag tac aag ttt caa 336
Tyr Gly Met Met Ile Thr Leu Gln Met Asn Asp Glu Tyr Lys Phe Gln
70 75 80 85
aag ctt tgg gac ttc gtt cgc aag tac atg cgc cat tca gac cgc aac 384
Lys Leu Trp Asp Phe Val Arg Lys Tyr Met Arg His Ser Asp Arg Asn
90 95 100
gac agc ctt tac ggc tat cac tct tgg cat atg aaa aca aac ggc tca 432
Asp Ser Leu Tyr Gly Tyr His Ser Trp His Met Lys Thr Asn Gly Ser
105 110 115
gac gtt caa acg atc gac caa aac gtt gcg tca gac ggc gag gta tgg 480
Asp Val Gln Thr Ile Asp Gln Asn Val Ala Ser Asp Gly Glu Val Trp
120 125 130
ttt gca gct gca ctt atg atg gct tct ggt cgc tgg ggt gac aag aag 528
Phe Ala Ala Ala Leu Met Met Ala Ser Gly Arg Trp Gly Asp Lys Lys
135 140 145
tac cct tac gac tac aag gca cgc gct caa gac atg ctt gac gca ctt 576
Tyr Pro Tyr Asp Tyr Lys Ala Arg Ala Gln Asp Met Leu Asp Ala Leu
150 155 160 165
gca ggc gac ggc gag tac gct aac aca ggc aag gag tct cgc gtt ttc 624
Ala Gly Asp Gly Glu Tyr Ala Asn Thr Gly Lys Glu Ser Arg Val Phe
170 175 180
atc aaa aac agc aag gac caa cgc tat gct atg gta cgc ttt ggc cct 672
Ile Lys Asn Ser Lys Asp Gln Arg Tyr Ala Met Val Arg Phe Gly Pro
185 190 195
tac gtt aac tgg act gac cca tct tat cat gtt ccg gct ttc ttc gag 720
Tyr Val Asn Trp Thr Asp Pro Ser Tyr His Val Pro Ala Phe Phe Glu
200 205 210
ctt ttc gcc aag tca gct aag tca tca caa caa tac ttc tgg aag gac 768
Leu Phe Ala Lys Ser Ala Lys Ser Ser Gln Gln Tyr Phe Trp Lys Asp
215 220 225
gca gct aac aaa tct cgc acg tac ctt agc gag aca act ttc aag tct 816
Ala Ala Asn Lys Ser Arg Thr Tyr Leu Ser Glu Thr Thr Phe Lys Ser
230 235 240 245
gta ctt aac aac ggc tca aca gtt act aac gct gct act ggc ctt ttt 864
Val Leu Asn Asn Gly Ser Thr Val Thr Asn Ala Ala Thr Gly Leu Phe
250 255 260
cct gac gag gct ggc ttt gac ggc gtt tca gac gct gct cat tct tca 912
Pro Asp Glu Ala Gly Phe Asp Gly Val Ser Asp Ala Ala His Ser Ser
265 270 275
aca gag aca gac cgc aac ttc tct tac gac gct tgg cgc act gta tct 960
Thr Glu Thr Asp Arg Asn Phe Ser Tyr Asp Ala Trp Arg Thr Val Ser
280 285 290
cat atc gct atg gac cat act ctt tgg agc tca gct gac aac gca tat 1008
His Ile Ala Met Asp His Thr Leu Trp Ser Ser Ala Asp Asn Ala Tyr
295 300 305
cgc gca tct gag caa aag gct gtt aac aag ttt ctt aca ttc atg aag 1056
Arg Ala Ser Glu Gln Lys Ala Val Asn Lys Phe Leu Thr Phe Met Lys
310 315 320 325
cgc gag aac tat ggt cgc act gct cat gag tat aca ctt aac ggc aca 1104
Arg Glu Asn Tyr Gly Arg Thr Ala His Glu Tyr Thr Leu Asn Gly Thr
330 335 340
gca gta aag aaa ggc tct cct gtt ggc ctt atc gca gca aac gct ggt 1152
Ala Val Lys Lys Gly Ser Pro Val Gly Leu Ile Ala Ala Asn Ala Gly
345 350 355
ggc gca acg gca gca tct gac gca tca ctt cgc acg ggc ttc gct aac 1200
Gly Ala Thr Ala Ala Ser Asp Ala Ser Leu Arg Thr Gly Phe Ala Asn
360 365 370
gcg ttt aac tct aca tac atc cca gag gac tac tac ggc tct tgt ctt 1248
Ala Phe Asn Ser Thr Tyr Ile Pro Glu Asp Tyr Tyr Gly Ser Cys Leu
375 380 385
tac atg ctt aac tct ctt gtt gcg aac ggc aag ttc gca atg tat ctt 1296
Tyr Met Leu Asn Ser Leu Val Ala Asn Gly Lys Phe Ala Met Tyr Leu
390 395 400 405
cca taa 1302
Pro
<210> 3
<211> 433
<212> PRT
<213> 人工序列
<220>
<223> 合成构建体
<400> 3
Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile
-25 -20 -15
Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Val His Ser Lys
-10 -5 -1 1 5
Thr Pro Asp Ile Leu Gly Thr Thr Gly Lys Asn Asn Leu Asn Gln Ala
10 15 20
Tyr Lys Lys Tyr Phe Asp Thr Lys Gly Asp Gly Lys Gly Gly Ser Leu
25 30 35
Phe His Tyr Met Lys Asp Gly Ser Ala Tyr Ile Ala Ser Thr Thr Asp
40 45 50
Asp Asn Leu Gly Asn Gly Tyr Tyr Ser Val Lys Thr Glu Gly Met Ser
55 60 65
Tyr Gly Met Met Ile Thr Leu Gln Met Asn Asp Glu Tyr Lys Phe Gln
70 75 80 85
Lys Leu Trp Asp Phe Val Arg Lys Tyr Met Arg His Ser Asp Arg Asn
90 95 100
Asp Ser Leu Tyr Gly Tyr His Ser Trp His Met Lys Thr Asn Gly Ser
105 110 115
Asp Val Gln Thr Ile Asp Gln Asn Val Ala Ser Asp Gly Glu Val Trp
120 125 130
Phe Ala Ala Ala Leu Met Met Ala Ser Gly Arg Trp Gly Asp Lys Lys
135 140 145
Tyr Pro Tyr Asp Tyr Lys Ala Arg Ala Gln Asp Met Leu Asp Ala Leu
150 155 160 165
Ala Gly Asp Gly Glu Tyr Ala Asn Thr Gly Lys Glu Ser Arg Val Phe
170 175 180
Ile Lys Asn Ser Lys Asp Gln Arg Tyr Ala Met Val Arg Phe Gly Pro
185 190 195
Tyr Val Asn Trp Thr Asp Pro Ser Tyr His Val Pro Ala Phe Phe Glu
200 205 210
Leu Phe Ala Lys Ser Ala Lys Ser Ser Gln Gln Tyr Phe Trp Lys Asp
215 220 225
Ala Ala Asn Lys Ser Arg Thr Tyr Leu Ser Glu Thr Thr Phe Lys Ser
230 235 240 245
Val Leu Asn Asn Gly Ser Thr Val Thr Asn Ala Ala Thr Gly Leu Phe
250 255 260
Pro Asp Glu Ala Gly Phe Asp Gly Val Ser Asp Ala Ala His Ser Ser
265 270 275
Thr Glu Thr Asp Arg Asn Phe Ser Tyr Asp Ala Trp Arg Thr Val Ser
280 285 290
His Ile Ala Met Asp His Thr Leu Trp Ser Ser Ala Asp Asn Ala Tyr
295 300 305
Arg Ala Ser Glu Gln Lys Ala Val Asn Lys Phe Leu Thr Phe Met Lys
310 315 320 325
Arg Glu Asn Tyr Gly Arg Thr Ala His Glu Tyr Thr Leu Asn Gly Thr
330 335 340
Ala Val Lys Lys Gly Ser Pro Val Gly Leu Ile Ala Ala Asn Ala Gly
345 350 355
Gly Ala Thr Ala Ala Ser Asp Ala Ser Leu Arg Thr Gly Phe Ala Asn
360 365 370
Ala Phe Asn Ser Thr Tyr Ile Pro Glu Asp Tyr Tyr Gly Ser Cys Leu
375 380 385
Tyr Met Leu Asn Ser Leu Val Ala Asn Gly Lys Phe Ala Met Tyr Leu
390 395 400 405
Pro
<210> 4
<211> 434
<212> PRT
<213> 芽孢杆菌属菌种KK-1
<220>
<221> 信号
<222> (1)..(28)
<220>
<221> 成熟肽
<222> (29)..(434)
<400> 4
Met Arg Lys Ser Leu Lys Trp Ile Met Ala Val Phe Ile Gly Leu Thr
-25 -20 -15
Cys Phe Cys Thr Ala Tyr Ser Gln Thr Ala Met Ala Ala Val His Ser
-10 -5 -1 1
Lys Thr Pro Asp Ile Leu Gly Thr Thr Gly Lys Asn Asn Leu Asn Gln
5 10 15 20
Ala Tyr Lys Lys Tyr Phe Asp Thr Lys Gly Asp Gly Lys Gly Gly Ser
25 30 35
Leu Phe His Tyr Met Lys Asp Gly Ser Ala Tyr Ile Ala Ser Thr Thr
40 45 50
Asp Asp Asn Leu Gly Asn Gly Tyr Tyr Ser Val Lys Thr Glu Gly Met
55 60 65
Ser Tyr Gly Met Met Ile Thr Leu Gln Met Asn Asp Glu Tyr Lys Phe
70 75 80
Gln Lys Leu Trp Asp Phe Val Arg Lys Tyr Met Arg His Ser Asp Arg
85 90 95 100
Asn Asp Ser Leu Tyr Gly Tyr His Ser Trp His Met Lys Thr Asn Gly
105 110 115
Ser Asp Val Gln Thr Ile Asp Gln Asn Val Ala Ser Asp Gly Glu Val
120 125 130
Trp Phe Ala Ala Ala Leu Met Met Ala Ser Gly Arg Trp Gly Asp Lys
135 140 145
Lys Tyr Pro Tyr Asp Tyr Lys Ala Arg Ala Gln Asp Met Leu Asp Ala
150 155 160
Leu Ala Gly Asp Gly Glu Tyr Ala Asn Thr Gly Lys Glu Ser Arg Val
165 170 175 180
Phe Ile Lys Asn Ser Lys Asp Gln Arg Tyr Ala Met Val Arg Phe Gly
185 190 195
Pro Tyr Val Asn Trp Thr Asp Pro Ser Tyr His Val Pro Ala Phe Phe
200 205 210
Glu Leu Phe Ala Lys Ser Ala Lys Ser Ser Gln Gln Tyr Phe Trp Lys
215 220 225
Asp Ala Ala Asn Lys Ser Arg Thr Tyr Leu Ser Glu Thr Thr Phe Lys
230 235 240
Ser Val Leu Asn Asn Gly Ser Thr Val Thr Asn Ala Ala Thr Gly Leu
245 250 255 260
Phe Pro Asp Glu Ala Gly Phe Asp Gly Val Ser Asp Ala Ala His Ser
265 270 275
Ser Thr Glu Thr Asp Arg Asn Phe Ser Tyr Asp Ala Trp Arg Thr Val
280 285 290
Ser His Ile Ala Met Asp His Thr Leu Trp Ser Ser Ala Asp Asn Ala
295 300 305
Tyr Arg Ala Ser Glu Gln Lys Ala Val Asn Lys Phe Leu Thr Phe Met
310 315 320
Lys Arg Glu Asn Tyr Gly Arg Thr Ala His Glu Tyr Thr Leu Asn Gly
325 330 335 340
Thr Ala Val Lys Lys Gly Ser Pro Val Gly Leu Ile Ala Ala Asn Ala
345 350 355
Gly Gly Ala Thr Ala Ala Ser Asp Ala Ser Leu Arg Thr Gly Phe Ala
360 365 370
Asn Ala Phe Asn Ser Thr Tyr Ile Pro Glu Asp Tyr Tyr Gly Ser Cys
375 380 385
Leu Tyr Met Leu Asn Ser Leu Val Ala Asn Gly Lys Phe Ala Met Tyr
390 395 400
Leu Pro
405
<210> 5
<211> 388
<212> PRT
<213> Burkholderia phymatum
<400> 5
Met Arg Arg Lys Arg Leu Leu Thr Gln Arg Phe Ala Arg Ile Pro Lys
1 5 10 15
Cys Gly Ala Val Arg Arg Leu Val Gly Leu Ala Val Ala Cys Leu Gln
20 25 30
Val Ser Val Ala Ala His Ala Asp Cys Arg Trp Pro Leu Trp Glu Arg
35 40 45
Tyr Ala Gln Gln Gln Ile Ser Lys Asp Gly Arg Ile Val Glu Pro Gly
50 55 60
Lys Gly Asp Arg Thr Thr Ser Glu Ala Gln Ala Tyr Ala Met Phe Phe
65 70 75 80
Ala Val Val Gly Asp Asp Arg Val Arg Phe Asp Ser Leu Leu Asn Trp
85 90 95
Ala Leu Ala His Thr Trp Ala Thr Thr Gly His Pro Pro Ala Trp Leu
100 105 110
Trp Ala Lys Arg Ala Asp Gly Thr Phe Asp Val Leu Asp Asp Asn Ser
115 120 125
Ala Thr Asp Ala Asp Leu Trp Met Ala Tyr Ala Met Leu Glu Ala Ala
130 135 140
Arg Arg Trp Ser Glu Pro Arg Tyr Arg Ala Leu Gly Leu Arg Leu Leu
145 150 155 160
Asp Gln Ile Thr Ala Thr Glu Val Val Trp Gln Asp Glu Thr Ser Ala
165 170 175
Phe Leu Ile Pro Gly Arg Leu Gly Phe Arg Leu Ser Glu His Ala Tyr
180 185 190
Leu Val Asn Pro Ser Tyr Ala Pro Ile Gln Leu Leu Arg Arg Phe Ile
195 200 205
Lys Glu Gln Pro His Gly Pro Trp Val Ser Val Leu Asn His Gln Val
210 215 220
Asp Leu Leu Val Ala Ser Ala Pro Gln Gly Phe Val Pro Asp Trp Tyr
225 230 235 240
Arg Phe Asp Thr Phe Lys Gly Tyr Met Leu Glu Pro Ser Lys Gly Pro
245 250 255
Thr Gly Gly Phe Asp Ala Val Arg Thr Tyr Met Trp Ala Gly Met Leu
260 265 270
Asp Pro Thr Asp Pro Ala Arg Gly Glu Val Ile Pro Ala Leu Ser Gly
275 280 285
Met Ala Asp Leu Val Ala Gln Thr Gly Arg Met Pro Leu Tyr Val Asn
290 295 300
Ile Glu Ser Gly Glu Thr Phe Asp Glu Gly Ser Ala Gly Phe Arg Ala
305 310 315 320
Ala Val Ile Pro Leu Leu Phe Val Thr Gly His Pro Ser Ala Ala Ser
325 330 335
Arg Leu Ala Gln Glu Thr Lys Val Met Gln Gln Ala Arg Glu Trp Asp
340 345 350
Gly Leu His Tyr Tyr Asp Arg Asn Leu Leu Leu Phe Ala Ala Gly Trp
355 360 365
Leu Glu Gly Arg Tyr Arg Phe Asp His Glu Gly Thr Leu Gln Leu Lys
370 375 380
Pro Cys Arg Pro
385
<210> 6
<211> 400
<212> PRT
<213> Labrenzia aggregata
<400> 6
Met Leu Phe Arg Phe Leu Ala Thr Leu Val Trp Cys Leu Leu Leu Leu
1 5 10 15
Leu Pro Val Ala Ala Arg Pro Leu Phe Pro Ser Gln Pro Gln Ala Glu
20 25 30
Arg Leu Ala Ala Leu Asp Arg Phe Trp Ala Asp Trp Lys Lys Thr Tyr
35 40 45
Leu His Glu Gly Cys Gly Gly Ala Tyr Val Asp Thr Ser Gly Asp Glu
50 55 60
Lys Pro Thr Trp Gly Gly Ser Ala Thr Gly Thr Leu Thr Val Ser Glu
65 70 75 80
Ala His Gly Tyr Gly Met Leu Thr Leu Val Lys Met Ala Ser Arg Asp
85 90 95
Pro Glu Ala Gln Glu Asn Phe Ala Ser Met Val Ala Phe Tyr His Ala
100 105 110
His Gln Ala Ala Ser Gly Pro Gly Leu Met Ala Trp Asn Gln Thr Arg
115 120 125
Asp Cys Lys Asp Ala Pro Asn Gly Gly Asp Met Ser Ala Thr Asp Gly
130 135 140
Asp Leu Asp Thr Ala Leu Ala Leu Leu Leu Ala Asp Lys Lys Trp Gly
145 150 155 160
Gly Tyr Arg Glu Ala Phe Asp Gln Ala Gln Gln Ala Ile Leu Glu Arg
165 170 175
Glu Val Thr Ala Asp Gly Met Met Arg Leu Gly Asp Trp Ala Val Asp
180 185 190
Glu Asp Tyr Ser Gly Thr Ser Arg Ser Ser Asp Phe Met Pro Ala Ser
195 200 205
Phe Ala Ala Phe Ala Lys Ala Ala Asp Asp Leu Ser Pro Arg Trp Thr
210 215 220
Ser Ile Arg Asp Arg Gly Tyr Arg Val Trp Gly Asp Ile Ala Glu Asn
225 230 235 240
Tyr Ala Gly Asn Thr Gly Leu Val Pro Asp Phe Leu Val Gly Leu Pro
245 250 255
Glu His Pro Arg Pro Ala Glu Ala Asp Phe Leu Glu Gly Ala Gly Asp
260 265 270
Gly Gln Tyr Ser Trp Asn Ala Leu Arg Phe Pro Trp Arg Leu Ser Leu
275 280 285
Asp Leu Leu Glu Thr Asp Asp Thr Arg Ala Ala Asp His Leu Met Val
290 295 300
Ile Asn Arg Trp Ile Arg Gly Ala Ala Lys Glu Asp Pro Phe Arg Ile
305 310 315 320
Ala Thr Thr Tyr Arg Leu Asp Gly Ser Ile Pro Glu Asp Gln Ser Thr
325 330 335
Gly Ser Ala Ala Phe Ile Ala Met Phe Ala Ala Ala Ala Ile Ala Gly
340 345 350
Ser Gly Asp Pro Asp Gly Asp Gln Val Trp Leu Asp Ala Leu Trp Ser
355 360 365
Ala Leu Ile Ser Ile Pro Leu Ala Asp Glu Asp Tyr Tyr Gly Asn Thr
370 375 380
Leu Lys Leu Leu Ala Met Ile Glu Leu Ser Gly Gln Trp Gln Glu Asn
385 390 395 400
<210> 7
<211> 376
<212> PRT
<213> Alteromonadales细菌TW-7
<400> 7
Met Lys Ser Leu Val Asn Met Phe Phe Thr Val Met Ala Leu Phe Ala
1 5 10 15
Phe Asn Ala Asn Ala Gln Val Glu Gln Cys Glu Pro Trp Gln Ser Trp
20 25 30
Gln Val Phe Lys Asn His Phe Ile Ser Gln Asp Gly Arg Val Ile Asp
35 40 45
Leu Gly Ser Asp Gln Ser Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala
50 55 60
Leu Phe Phe Ala Leu Ile Ala Asn Asp Lys Ala Ala Phe Asp Ser Val
65 70 75 80
Leu Asn Trp Thr Gln Glu His Leu Ser Glu Gly Asp Leu Ser Thr Arg
85 90 95
Leu Pro Ala Trp Lys Trp Gly Leu Asp Lys Asn Arg Val Gly Gly Ile
100 105 110
Leu Asp Ser Asn Pro Ala Ser Asp Ser Asp Leu Trp Ile Ala Tyr Ser
115 120 125
Leu Ser Gln Ala Ala Ile Leu Trp Asp Glu Arg Arg Tyr Ser Ile Leu
130 135 140
Ala Ala Val Leu Ala Gln Arg Ile Ile Arg Glu Glu Thr Ala Tyr Ile
145 150 155 160
Thr Gly Leu Gly Leu Ser Leu Leu Pro Ala Pro Ala Gly Phe Glu Phe
165 170 175
Asp Asn Glu Arg Tyr Lys Leu Asn Pro Ser Tyr Ser Pro Leu Phe Ile
180 185 190
Tyr Gln Gln Phe Lys Lys Leu Tyr Pro His Ser Pro Trp His Gln Leu
195 200 205
His Glu Gly Ser Ala Lys Leu Ile Leu Asp Thr Ala Gln Gln Gly Val
210 215 220
Ser Pro Asp Trp Val Met Phe Asp Ser Asn Arg Gly Phe Tyr Tyr Asp
225 230 235 240
Arg Lys Asn Thr Asp Leu Gly Ser Tyr Asn Ala Ile Arg Val Tyr Leu
245 250 255
Trp Ala Ser Met Met Ala Lys Asp Ala Pro Tyr Lys Gln Glu Leu Leu
260 265 270
Ala Gln Phe Ala Pro Phe Val Asp Thr Ile Lys Glu Arg Ser His Val
275 280 285
Pro Leu Asn Thr Tyr Ala Gln Ser Gly Lys Ser Glu Lys Arg Gly Pro
290 295 300
Leu Gly Phe Asn Ala Ala Leu Leu Pro Leu Leu Ala Glu Gln Gly Asn
305 310 315 320
Asp Glu Leu Val Met Ala Ile Gln Gln Lys Leu Met Ile Asp Lys Ser
325 330 335
Phe Thr Gln Thr Arg Tyr Tyr Asp Ser Val Leu His Leu Phe Gly Glu
340 345 350
Ser Ala Val Asn Lys Arg Phe Lys Ile Thr Ala Asp Gly Thr Leu Gln
355 360 365
Pro Thr Trp Ser Thr Glu Cys Arg
370 375
<210> 8
<211> 375
<212> PRT
<213> 小肠结肠炎耶尔森氏菌
<400> 8
Met Val Val Met Phe Lys Arg Leu Val Cys Ile Leu Leu Leu Leu Ala
1 5 10 15
Ser Phe Gly Thr Ala Ala Ala Cys Asp Trp Pro Ala Trp Gln Gln Phe
20 25 30
Lys Gln His Tyr Ile Ser Glu Ser Gly Arg Val Ile Asp Pro Ser Asn
35 40 45
Pro Arg Lys Val Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Val Ala Asp Asp Arg Glu Thr Phe Asp Arg Leu Leu Thr Trp
65 70 75 80
Thr Glu Asn Asn Leu Ser Ala Gly Asp Leu Thr Ala Arg Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Arg Asn Glu His Ser Trp Asp Ile Leu Asp Pro
100 105 110
Asn Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Asp Thr Arg Arg Tyr Gln Thr Ile Gly Thr Leu
130 135 140
Leu Leu Gln Arg Ile Ala Lys Glu Glu Val Val Asn Ile Pro Gly Leu
145 150 155 160
Gly Glu Met Leu Leu Pro Gly Lys Ile Gly Phe Asn Asn Glu Glu Arg
165 170 175
Trp Arg Leu Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala Arg Phe
180 185 190
Ala Ser Leu Ala Gly Pro Trp Lys Ala Met Val Lys Thr Asn Gln Arg
195 200 205
Leu Trp Leu Glu Thr Ala Pro Arg Gly Phe Ser Pro Asp Trp Val Val
210 215 220
Trp Gln Lys Asp Lys Gly Trp Gln Pro Asp Glu Thr His Pro Asn Leu
225 230 235 240
Gly Ser Tyr Asp Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Ser
245 250 255
Asn Asn Ser Pro Gln Lys Ala Ala Leu Ile Gln His Phe Gln Pro Met
260 265 270
Ala Glu Val Thr Gln Gln Gln Gly Leu Pro Pro Glu Lys Ala Asp Thr
275 280 285
Leu Thr Gly Lys Thr Thr Gly Asp Gly Pro Val Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Phe Leu Ala Ser Gly Pro Ala Pro Phe Asn Gln Gln Ala
305 310 315 320
Leu Ser Gln Gln Gln Lys Arg Val Gln Asp Ser Pro Pro Gly Ala Asp
325 330 335
Ala Tyr Tyr Ser Ala Val Leu Thr Leu Phe Gly Gln Gly Trp Ala Gln
340 345 350
His Arg Tyr Ser Phe Asn Arg Gln Gly Glu Leu Gln Pro Ser Trp Asn
355 360 365
Ser Gln Cys Ala Thr Leu Lys
370 375
<210> 9
<211> 376
<212> PRT
<213> Mycobacterium vanbaalenii
<400> 9
Met Val Ser Arg Leu Arg Lys Ala Met Leu Leu Leu Val Ala Leu Leu
1 5 10 15
Leu Val Val Gly Ala Gly Trp Ala Ala Ala Gln Ala Val Asp Pro Arg
20 25 30
Met Ser Asp Asp Asp Val Arg Gly Met Arg Glu Gln Ala Ala Arg Gln
35 40 45
Ala Gly Glu Asp Phe Leu Ser Arg Tyr Val Glu Ser Asp Gly Arg Val
50 55 60
Val Arg Arg Asp Glu Gly Gly Asp Val Val Ser Glu Gly Gln Ala Tyr
65 70 75 80
Gly Met Leu Ile Ala Ala Ala Leu Gly Asp Glu Pro Arg Phe Arg Ala
85 90 95
Ile Trp Asp Trp Thr Arg Thr His Leu Arg Arg Pro Asp Gly Leu Leu
100 105 110
Ser Trp Arg Trp Ala Asp Gly Arg Val Thr Asp Pro Ser Ser Ala Thr
115 120 125
Asp Ala Asp Leu Asp Ala Ala Arg Ser Leu Leu Leu Ala Ala Arg Arg
130 135 140
Phe Ala Ala Pro Glu Leu Ala Glu Asp Gly Lys Arg Leu Gly Ala Asp
145 150 155 160
Val Leu Arg Gly Glu Thr Val Thr Val Gly Ala Ala Pro Ser Pro Ala
165 170 175
Met Ala Arg Pro Gly Leu Ile Thr Val Ala Gly Asn Trp Ala Thr Ala
180 185 190
Pro Pro His Ala Val Asp Pro Gly Tyr Phe Ser Arg Arg Ala Glu Arg
195 200 205
Glu Leu Leu Asp Ala Ser Ala Asp Arg Arg Trp Leu Asp Val Ser Arg
210 215 220
Thr Gln Arg Val Leu Val Trp Gln Leu Ile Gly Thr Ala Ser Leu Pro
225 230 235 240
Pro Asp Trp Ala Ser Val Asp Pro Ala Gly Arg Ala Val Pro Thr Gly
245 250 255
Pro Pro Asp Gly Gly Pro Thr Arg Phe Gly Leu Asp Ala Ala Arg Leu
260 265 270
Pro Ile Arg Phe Ala Glu Ser Cys Asp Pro Ala Asp Arg Ala Val Ala
275 280 285
Val Ser Leu Arg Arg Val Val Ala Ala Ser Arg Asp Ile Pro Ala Thr
290 295 300
Arg Asn Leu Asp Gly Ser Ala Ala Gly Glu Trp Gln His Pro Val Ala
305 310 315 320
Leu Val Ser Ala Ala Ala Thr Asp His Ala Ala Gly Asp Arg Glu Ala
325 330 335
Gly Ala Ala Arg Leu Asp Gln Ala Ser Ala Leu Gln Gln Arg Tyr Pro
340 345 350
Thr Tyr Phe Gly Ala Ala Trp Val Ala Leu Gly Arg Ile Met Leu Asp
355 360 365
Thr Ser Leu Leu Gly Glu Cys Ala
370 375
<210> 10
<211> 298
<212> PRT
<213> Lactococcus lactis
<400> 10
Met Lys Asn Leu Leu Leu Lys Val Ala Ser Lys Glu Ala Ile Asp Lys
1 5 10 15
Lys Val Glu Lys Val Phe Glu Ile Leu Phe Gly Gln Asp Ser Pro Glu
20 25 30
Arg Leu Tyr Phe Glu Glu Glu Asp Lys Ala Tyr Ile Val Asp Thr Gly
35 40 45
Asn Asp Asp Val Arg Thr Glu Gly Met Ser Tyr Gly Met Met Ile Ala
50 55 60
Leu Gln Leu Asp Lys Pro Glu Ile Phe Ser Arg Leu Trp Ala Trp Val
65 70 75 80
Lys Thr Tyr Met Thr Val Pro Lys Gly His Glu Asn Glu Gly Tyr Phe
85 90 95
Ile Trp Ser Cys Gly Leu Asp Gly His Pro Asn Ser Asp Gly Pro Ala
100 105 110
Pro Asp Gly Glu Glu Tyr Phe Ala Ala Ala Leu Leu Leu Ala Glu Lys
115 120 125
Arg Trp Lys Ile Lys Glu Tyr Gly Asp Glu Ala Arg Ala Leu Leu His
130 135 140
Ala Met Val His Lys Gly Glu Asn Gln Asp Gly Tyr Pro Met Phe Glu
145 150 155 160
Pro Lys Asn Thr Tyr Ile Lys Phe Val Ala Asn Arg Gln Met Thr Asp
165 170 175
Pro Ser Tyr His Leu Leu His Phe Tyr Gln Leu Tyr Ala Lys Tyr Gly
180 185 190
Asn Pro Glu Asp Ser Ala Phe Phe Leu Lys Ala Glu Glu Glu Ala Arg
195 200 205
Lys Tyr Trp Leu Lys Ser Ala Asn Ala Lys Thr Gly Leu Thr Pro Glu
210 215 220
Tyr Ala Asp Tyr Asp Gly Lys Pro Tyr Asp Ile Asp Gly His Trp Thr
225 230 235 240
Phe Phe Ser Asp Ala Tyr Arg Thr Ala Ala Asn Ile Gly Leu Asp Trp
245 250 255
Ile Trp Glu His Lys Asp Ile Gly Gln Ser Gln Ile Ala Leu Asn Ile
260 265 270
Gln Lys Phe Phe Glu Ile Tyr Leu Asn Ser Asp Lys Glu Ile Pro Val
275 280 285
Phe Lys Ile Asn Gly Gln Pro Leu Arg Lys
290 295
<210> 11
<211> 409
<212> PRT
<213> Burkholderia cenocepacia
<400> 11
Met Ala Arg Ala Met Thr Lys Arg Arg Ala Thr Gln Pro Ala Arg Arg
1 5 10 15
Val Gly Ala Thr Leu Ala Leu Ala Val Ala Val Thr Cys Ala Ala Ala
20 25 30
Gly Met Ala Thr Arg Thr His Ala Ala Ala Ala Ser Ala Ala Asp Ala
35 40 45
Ala Ser Thr Gly Cys Ser Val Ala Trp Pro Arg Trp Asp Ala Phe Lys
50 55 60
Arg Asp Phe Ile Ser Ala Asp Gly Arg Val Ile Asp Val Gly Ser Ala
65 70 75 80
Asp Ser Arg Thr Val Ser Glu Gly Gln Ala Tyr Gly Leu Phe Leu Ala
85 90 95
Leu Val Ala Asn Asp Arg Arg Met Phe Asp Thr Ile Leu Ala Trp Thr
100 105 110
Glu Asn Asn Leu Ala Gln Gly Asp Leu Ser Ala His Leu Pro Ala Trp
115 120 125
Leu Trp Gly Arg Ala Pro Asp Gly Ala Trp Arg Val Leu Asp Ala Asn
130 135 140
Pro Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ala Leu Val Glu Ala
145 150 155 160
Gly Arg Leu Trp His Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu
165 170 175
Ala Lys Arg Val Leu Asp Ala Glu Thr Ala Thr Val Pro Gly Leu Gly
180 185 190
Leu Thr Leu Leu Pro Gly Pro Thr Gly Phe Lys Leu Ala Asn Gly Gln
195 200 205
Trp Arg Leu Asn Pro Ser Tyr Ser Pro Pro Gln Val Ile Arg Gly Leu
210 215 220
Gly Ala Arg Leu Pro Asp Asp Arg Ala Leu Gly Arg Ala Gly Val Gln
225 230 235 240
His Cys Ser Arg Ala Ala Arg His Arg Ala Glu Arg Phe Ser Pro Asp
245 250 255
Trp Ala Leu Tyr Arg Ala Gly Asn Gly Phe Gly Pro Asp Ser Gln Thr
260 265 270
His Ala Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly
275 280 285
Met Leu Asp Arg Ala Asp Pro Leu Ala Ala Pro Leu Leu Ala Lys Phe
290 295 300
Ala Pro Phe Ala Asp His Ile Ala Val His Gly Ala Pro Pro Glu Lys
305 310 315 320
Val Asp Thr Thr Thr Gly Val Ala Gly Pro Asn Asp Gly Asn Gly Gly
325 330 335
Phe Ser Ala Ala Ala Val Pro Phe Leu Asp Ala Arg Gly Gln His Ala
340 345 350
Leu Ala Asp Ala Gln Ala Thr Arg Val Asp Thr Leu Ala Arg Gln Ser
355 360 365
Ala Pro Gly Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu Gly Trp
370 375 380
Arg Asp Gly Arg Tyr Arg Phe Gly Thr Asp Gly Thr Leu Asp Thr Arg
385 390 395 400
Trp Gly Gly Arg Ser Cys Ala Ala Arg
405
<210> 12
<211> 342
<212> PRT
<213> Streptococcus sanguinis
<400> 12
Met Gly Ser Thr Pro Asn Ile Lys Lys Lys Ile Tyr Ser Gln Trp Ala
1 5 10 15
Lys Glu Tyr Val Val Thr Lys Asp Lys Leu Ser Tyr Ile Arg Thr Thr
20 25 30
Asn Ser Lys Thr Glu Asp Val Val Leu Ser Glu Ala Gln Gly Tyr Gly
35 40 45
Met Val Ile Ala Val Asp Ala Ala Lys Gln Gly Asp Ala Ser Ser Ala
50 55 60
Asp Phe Glu Lys Leu Tyr Gln Tyr Tyr Leu Ala His Arg Leu Lys Asp
65 70 75 80
Thr Gln Leu Met Ser Trp Lys Gln Thr Ile Lys Asp Gly Lys Ser Asn
85 90 95
His Glu Asp Glu Asn Asn Ala Thr Asp Gly Asp Leu Tyr Ile Ala Tyr
100 105 110
Ala Leu Ile Gln Ala Ala Lys Gln Trp Pro Asp Lys Ala Lys Glu Tyr
115 120 125
Gln Asp Gln Ala Gln Ala Ile Leu Lys Asp Val Leu Ala Tyr Asn Tyr
130 135 140
Asn Glu Ser Asn Gly Val Leu Thr Val Gly Asn Trp Ala Asn Ala Glu
145 150 155 160
Ser Lys Phe Tyr Asn Leu Met Arg Thr Ser Asp Thr Leu Pro Gln Gln
165 170 175
Phe Gln Ala Phe Tyr Glu Leu Thr Lys Asp Lys Gln Trp Leu Thr Ile
180 185 190
Arg Asp Asn Met Leu Ser Lys Leu Glu Ala Ile Ser Ala Asp Asn Lys
195 200 205
Thr Gly Leu Ile Pro Asp Phe Ile Trp Val Glu Gly Asp Lys Val Arg
210 215 220
Ala Ala Asp Ala Asp Thr Val Glu Ser Ala Asn Asp Gly Tyr Tyr Ser
225 230 235 240
Tyr Asn Ala Cys Arg Leu Pro Tyr Asn Leu Ala Gln Ser Lys Asp Glu
245 250 255
Lys Ser Gln Lys Met Leu Lys Lys Met Leu Asn Phe Phe Leu Ser Gln
260 265 270
Glu Lys Ile Tyr Ala Gly Tyr Thr Leu Lys Gly Lys Ala Leu Asn Ser
275 280 285
Asn Gln Ala Gly Ser Phe Thr Ala Pro Val Phe Tyr Ala Ala Asn Asn
290 295 300
Asn Met Glu Phe Arg Lys Leu Val Gln Gln Asn Lys Tyr Leu Phe Met
305 310 315 320
Gln Gly Leu Pro Ser Asp Asn Tyr Tyr Asp Ala Ala Val Thr Thr Met
325 330 335
Ile Ala Leu Glu Thr Leu
340
<210> 13
<211> 366
<212> PRT
<213> Leptospirillum rubarum
<400> 13
Met Lys Pro Met Ile Leu Gly Val Met Ile Val Trp Phe Phe Val Trp
1 5 10 15
Gly Ser Gly Leu Ser Arg Ala Ser Glu Phe His Ala Val Trp Glu His
20 25 30
Tyr Arg Ala Cys Cys Ile Gln Ser Asp Gly Arg Val Ile Asp Arg Gly
35 40 45
Arg Gln Ser Val Thr Thr Ser Glu Gly Glu Ala Tyr Ala Leu Phe Phe
50 55 60
Ala Leu Val Asp Asn Asp Pro Leu Leu Tyr Ser Arg Leu Leu Arg Trp
65 70 75 80
Thr Arg Asp Asn Leu Ser Arg Gly Asp Leu Ser Ala His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Glu Arg Lys Asn Gly Gln Trp Gly Val Leu Asp Gln
100 105 110
Asn Ser Ala Thr Asp Ala Asp Leu Trp Ile Ser Phe Asp Leu Met Glu
115 120 125
Ala Gly Arg Leu Trp His Arg Ser Glu Tyr Leu Arg Leu Gly Glu Arg
130 135 140
Leu Ala Phe Leu Ile Ser Leu Arg Asp Phe Ala Asn Leu Pro Gly Phe
145 150 155 160
Gly Ala Phe Pro Leu Gly Gly Glu Arg Gly Phe His Pro Ala Gly Thr
165 170 175
Val Trp Arg Thr Asn Pro Ser Tyr Phe Pro Pro Phe Leu Leu Arg Phe
180 185 190
Met Glu His Arg Phe Gly Gly Arg Pro Trp Ser Gly Leu Pro Gly Arg
195 200 205
Phe Leu Ala Phe Val Lys Ser Val Ser Ser Cys Gly Phe Val Pro Asp
210 215 220
Trp Val Thr Tyr Asp Thr Gly Lys Gly Trp Gln Pro Asp Pro Val Thr
225 230 235 240
Gly Pro Leu Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly
245 250 255
Met Thr Ser Val Arg Asp Pro Gln Glu Gln Leu Leu Leu Lys Arg Leu
260 265 270
Gln Gly Trp Ser Arg Lys Gly Arg Ser Ser Pro Thr Leu Phe Val Asn
275 280 285
Thr Lys Thr Cys Ser Ala Tyr Gly Lys Pro Pro Pro Gly Phe Arg Ala
290 295 300
Ala Tyr Leu Pro Phe Leu Arg Arg Glu Asn Val Ser Ser Phe Arg Glu
305 310 315 320
Glu Phe Arg Arg Met Leu Ser Arg Asp Pro Met Lys Asn Asp Pro Gly
325 330 335
Tyr Tyr Asp Thr Asn Leu Tyr Leu Phe Gly Thr Gly Phe Leu Glu Lys
340 345 350
Arg Phe Ser Phe Asp Glu Arg Gly Arg Leu His Val Arg Trp
355 360 365
<210> 14
<211> 416
<212> PRT
<213> Sagittula stellata
<400> 14
Met Gly Asn Ile Arg Ser Arg Ser Gly Tyr Ala Ile Val Ala Phe Ser
1 5 10 15
Val Ser Ala Ala Trp Thr Thr Leu Ala Ala Asp Val Ala Ala Gly Pro
20 25 30
Thr Leu Pro Val Ala Pro Glu Asp Ala Ser Val Ile Tyr Pro Ser Val
35 40 45
Asp Lys Asp His Arg Arg Ala Val Leu Asp Arg Phe Trp Arg Glu Trp
50 55 60
Lys Arg Val Tyr Leu His Gln Gly Cys Gly Gly Ala Tyr Val Asp Ile
65 70 75 80
Ala Gly Asp Gly Lys Pro Thr Tyr Gly Asp Ser Val Pro Asn Thr Leu
85 90 95
Thr Val Ser Glu Ala His Gly Tyr Gly Met Leu Ala Leu Val Arg Met
100 105 110
Ala Gly Arg Asp Thr Gln Ala Lys Pro Leu Phe Asp Glu Met Leu Ala
115 120 125
Tyr Phe Arg Leu His Pro Ala Glu Ser Gly Pro Gly Leu Met Ala Trp
130 135 140
Asn Gln Thr Arg Asp Cys Lys Asp Ala Pro Asp Gly Glu Met Thr Ala
145 150 155 160
Ser Asp Gly Asp Ile Asp Ile Ala Leu Ala Leu Gln Met Ala Glu Arg
165 170 175
Val Trp Gly Gly Tyr Ala Glu Asp Ala Ala Glu Val Arg Glu Ala Val
180 185 190
Leu Ser Arg Glu Ile Thr Arg His Asp Leu Val Lys Leu Gly Asp Trp
195 200 205
Ala Ser Tyr Asp Val Tyr Ala Glu Ala Ser Arg Ser Ser Asp Phe Thr
210 215 220
Pro Phe Asn Phe Phe Ala Phe Ala Asp Ala Pro Gly Gly Asp Ala Thr
225 230 235 240
Arg Trp Thr Asp Ile Arg Asn Thr Gly Tyr Ala Val Trp Gly Gln Ile
245 250 255
Ser Glu Thr Tyr Ala Pro Asp Thr Gly Leu Val Pro Asp Phe Met Val
260 265 270
Gly Met Pro Asn Asp Pro Arg Pro Ala Pro Ala Glu Phe Leu Glu Gly
275 280 285
Glu Tyr Asp Gly Phe Tyr Ser Trp Asn Ala Leu Arg Tyr Pro Tyr Arg
290 295 300
Leu Ala Ala Asp Phe Arg Val Ser Gly Asp Pro Arg Ala Ala Glu Arg
305 310 315 320
Leu Arg Arg Ile Asn Lys Trp Ile Arg Ala Ala Thr Asn Gly Glu Pro
325 330 335
Ser Arg Ile Ala Ser Thr Tyr Gln Leu Asp Gly Ser Ile Pro Gln Asp
340 345 350
Gly Arg Trp Thr Gly Glu Pro Gly Trp Ile Ser Met Phe Thr Ala Gly
355 360 365
Ala Ile Ala Gly Ser Gly Asp Pro Thr Ala Asp Gln Ala Trp Met Asp
370 375 380
Ser Leu Trp Ala Ala Met Val Ser Ile Pro Ile Glu Glu Gly Asp Tyr
385 390 395 400
Phe Gly Asn Thr Leu Lys Leu Leu Ala Met Ile Asp Leu Ala Glu Met
405 410 415
<210> 15
<211> 437
<212> PRT
<213> 鼻疽伯克霍尔德氏菌
<400> 15
Met Ala Ser Phe Ser Val Met Ala Phe Ala Ala Ala Thr Leu Pro Val
1 5 10 15
Ser Trp Arg Val Ala Ala Ala Ala Glu Arg Thr Arg Ser Gly Gly Glu
20 25 30
Arg Ala Gly Gly Leu Arg Asp Thr Ala Gly Leu Ile Glu Ile Ser Ala
35 40 45
Ala Ala Pro Ala Ser Thr Pro Ile Pro Ala Ala Pro Arg Arg Phe Ala
50 55 60
Gln Pro Phe Ala Gln Pro Ala Arg Ala Phe Ala Val Ala Ser Ala Cys
65 70 75 80
Ala Pro Ser Trp Pro Arg Trp Asp Arg Phe Lys Arg Asp Phe Val Ser
85 90 95
Ala Asp Gly Arg Val Ile Asp Val Gly Ser Ala Asp Glu Arg Thr Val
100 105 110
Ser Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val Ala Asn Asp
115 120 125
Arg Ala Ala Phe Asp Ala Leu Leu Arg Trp Thr Glu Asp Asn Leu Ala
130 135 140
Gln Gly Asp Leu Ser Ala Arg Leu Pro Ala Trp Leu Trp Gly Arg Ala
145 150 155 160
Ala Asp Gly Ala Trp Arg Val Leu Asp Ala Asn Ala Ala Ser Asp Ala
165 170 175
Asp Leu Trp Leu Ala Tyr Ala Leu Leu Glu Ala Gly Arg Leu Trp Arg
180 185 190
Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu Ala Lys Arg Val Leu
195 200 205
Asp Glu Glu Thr Ala Thr Leu Pro Gly Leu Gly Leu Val Leu Leu Pro
210 215 220
Gly Pro Met Gly Phe Arg Pro Ala Arg Asp Ala Trp Arg Leu Asn Pro
225 230 235 240
Ser Tyr Ser Pro Pro Gln Ala Ile Arg Gly Ile Gly Ala His Val Pro
245 250 255
Asp Asp Ala Arg Trp Ala Arg Leu Ala Ala Gly Phe Gly Arg Val Leu
260 265 270
Thr Asp Ser Ala Pro Arg Gly Phe Ala Pro Asp Trp Ala Leu Tyr Arg
275 280 285
Ala Gly Arg Gly Phe Glu Pro Asp Ala Glu Thr His Ala Val Ser Ala
290 295 300
Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Leu Asp Ala Gly
305 310 315 320
Asp Pro Leu Ala Arg Pro Leu Val Ala His Phe Ala Pro Phe Ala Glu
325 330 335
His Val Ala Ala His Gly Ala Pro Pro Glu Ala Val Asp Ala Thr Thr
340 345 350
Gly Ala Ala Ala Pro Arg Asp Gly Asn Ala Gly Phe Ser Ala Ala Ala
355 360 365
Val Pro Phe Leu Glu Ala Arg Gly Glu Arg Ala Ser Ala Asp Ala Gln
370 375 380
Leu Ala Arg Val Ala Arg Leu Glu Arg Glu Thr Ala Ser Gly Tyr Tyr
385 390 395 400
Ala Asn Val Leu Thr Leu Phe Gly Leu Gly Trp Arg Asp Gly Arg Tyr
405 410 415
Arg Phe Ala Ala Asp Gly Thr Leu Arg Val Arg Trp Ser Glu Pro Cys
420 425 430
Ser Thr Pro Ala Arg
435
<210> 16
<211> 356
<212> PRT
<213> 类球红细菌
<400> 16
Met Arg Arg Arg Thr Ile Leu Thr Ser Ala Ala Ala Ala Leu Met Leu
1 5 10 15
Ala Pro Ala Gly Arg Leu Phe Ala Gln Ser Gly Gly Glu Ala Leu Ser
20 25 30
Ala Asp His Pro Leu Gln Ala Ala Trp Arg Ser Trp Lys Asp Ala Phe
35 40 45
Leu Leu Pro Ala Gly Arg Ile Val Asp Gly Pro Gln Gln Asn Ala Ser
50 55 60
His Ser Glu Gly Gln Gly Tyr Gly Ala Thr Leu Ala Ala Ile Phe Gly
65 70 75 80
Asp Glu Glu Ala Leu Arg Arg Ile Val Asp Trp Thr Glu Ala Asn Leu
85 90 95
Ala Arg Arg Glu Asp Lys Leu Leu Ser Trp Arg Trp Leu Pro Gly Val
100 105 110
Ala Leu Ala Val Pro Asp Glu Asn Asn Ala Thr Asp Gly Asp Leu Phe
115 120 125
Tyr Ala Trp Gly Leu Ala Met Ala Ala Gln Arg Phe Gly Lys Ala Asp
130 135 140
Tyr Ala Gly Arg Ala Thr Glu Leu Ala Arg Ala Ile Ala Leu His Cys
145 150 155 160
Val Arg Pro His Pro Asp Gly Ser Glu Gln Leu Val Leu Leu Pro Gly
165 170 175
Ala Ser Gly Phe Glu Thr Pro Asp Gly Val Val Leu Asn Pro Ser Tyr
180 185 190
Tyr Met Pro Arg Ala Leu Thr Glu Leu Ala Ala Phe Ser Gly Gln Asp
195 200 205
Arg Leu Ala Arg Cys Ala Arg Asp Gly Ala Asp Trp Ile Ala Ser Leu
210 215 220
Gly Leu Pro Pro Asp Trp Ala Leu Val Thr Pro Phe Gly Thr Gln Pro
225 230 235 240
Ala Pro Gly Leu Ser His Asn Ser Gly Tyr Asp Ala Leu Arg Val Pro
245 250 255
Leu Phe Leu Leu Trp Ser Gly Leu Thr Ala Asn Pro Ala Leu Arg Arg
260 265 270
Ala Val Glu Ala Ala Gly Asp Ala Ala Ala Gly Asp Thr Pro Val Arg
275 280 285
Phe Asp Arg Asp Thr Gly Ala Val Leu Glu Arg Ser Ala Asp Pro Gly
290 295 300
Phe Arg Ala Val Leu Ala Leu Gly Asp Cys Ala Leu Ser Gly Arg Pro
305 310 315 320
Gly Ala Ala Ile Pro Pro Phe Asp Ala Arg Gln Pro Tyr Tyr Pro Ala
325 330 335
Thr Leu His Leu Met Ala Leu Val Ala Gln Val Glu Gly Phe Ser Ala
340 345 350
Cys Val Pro Ile
355
<210> 17
<211> 342
<212> PRT
<213> Rhodobacterales细菌
<400> 17
Met Lys Leu Thr Arg Arg Arg Leu Ile Ala Thr Leu Thr Gly Tyr Ala
1 5 10 15
Leu Pro Met Ala Phe Leu Thr Ser Val Arg Ala Asp Glu Arg Ala Ala
20 25 30
Trp Asp Val Trp Lys Ser Arg Phe Leu Ala Gly Asn Gly Arg Val Ile
35 40 45
Asp His Leu Gln Asn Gly Val Ser His Ser Glu Gly Gln Gly Tyr Gly
50 55 60
Leu Leu Leu Ala Gln Ala Phe Gly Asp Arg Glu Ala Phe Asp Ala Ile
65 70 75 80
Glu Ser Trp Thr Arg Glu His Leu Leu Ile Arg Asp Asp Ala Leu Met
85 90 95
Ala Trp Arg Trp Ser Pro Ser Gly Asn Arg Asp Asp Glu Leu Ala Gly
100 105 110
Asn Asp Trp His Thr Ala Thr Asp Gly Asp Leu Phe Arg Ala Trp Ala
115 120 125
Leu Met Arg Ser Glu Val Phe Ser Gly Trp Gly Asp Arg Arg Gly Thr
130 135 140
Val Glu Ala Ile Ser Asn Ala Leu Ala Ala Leu Cys Leu Glu Pro Asp
145 150 155 160
Pro Arg Ala Pro Asp Glu Pro Ile Leu Thr Pro Gly Ala Glu Ala Met
165 170 175
Ser Glu Pro Asp Arg Val Leu Phe Asn Pro Ser Tyr Ile Met Pro Arg
180 185 190
Ala Leu Arg Glu Leu Gly Leu Tyr Thr Glu Arg Glu Thr Leu Ile Arg
195 200 205
Ala Ala Asp His Gly Glu Thr Ile Leu Ala Glu Leu Ala Ala Thr Gly
210 215 220
Leu Val Pro Asp Trp Val Leu Val Thr Arg Asp Gly Phe Arg Pro Ala
225 230 235 240
Glu Gly Phe Gly Ala Asn His Gly Tyr Asp Ala Ile Arg Val Ala Leu
245 250 255
Tyr Leu His Trp Ser Gly Asn Ala Ala His Pro Ala Arg Thr Gln Lys
260 265 270
Gln Glu Ile Trp Thr Gly Glu Ala Arg Val Pro Val Val Leu Thr Pro
275 280 285
Gln Gly Glu Val Leu Glu Arg Ser Thr Tyr Pro Gly Tyr Arg Ala Val
290 295 300
Ala Asp Leu Ile Arg Cys Ser Pro Asn Asp Pro Val Glu Asp Ala Gly
305 310 315 320
Glu Pro Tyr Tyr Pro Ala Thr Leu Gly Leu Met Ala Ser Val Ala Arg
325 330 335
Arg Glu Ser Gly Leu Cys
340
<210> 18
<211> 380
<212> PRT
<213> 类芽孢杆菌属菌种W-61
<400> 18
Met Asn Ile Thr Gly Gln Gly Ala Tyr Asp Thr Asn Thr Tyr Asn Asn
1 5 10 15
Leu Phe His Lys Phe Gly Tyr Asp Glu Gln Glu Ile Asn Ala Arg Leu
20 25 30
Glu Glu Ser Trp Asn Glu Leu Phe Asp Gly Glu Glu Asp Thr Arg Ile
35 40 45
Tyr Tyr Pro Met Gly Glu Asp Lys Gly Tyr Leu Leu Asp Thr Gly Asn
50 55 60
Asn Asp Val Arg Ser Glu Gly Met Ser Tyr Gly Met Met Met Ala Val
65 70 75 80
Gln Met Asp Lys Lys Glu Glu Phe Asp Arg Leu Trp Asn Phe Ser His
85 90 95
Ala Phe Met Gln His Ala Glu Gly Arg Tyr Lys Asp Tyr Phe Ala Trp
100 105 110
His Cys Lys Pro Asp Gly Thr Arg Leu Ser Gln Gly Pro Ala Pro Asp
115 120 125
Gly Glu Glu Phe Phe Ala Met Ala Leu Phe Phe Ala Ser Asn Arg Trp
130 135 140
Gly Asp Gly Asp Ala Pro Leu Asn Tyr Lys Glu Gln Ala Arg Arg Ile
145 150 155 160
Leu Arg Ala Cys Ile His Gln Gly Glu Asn Gly Glu Gly Asp Pro Met
165 170 175
Trp Asp Pro Glu Thr Lys Leu Ile Lys Phe Val Pro Glu Ser Pro Phe
180 185 190
Ser Asp Pro Ser Tyr His Leu Pro His Phe Tyr Glu Leu Phe Ala Leu
195 200 205
Tyr Ala Asp Glu Asp Asp Gln Ser Phe Trp Arg Asp Ala Ala Ala Ala
210 215 220
Ser Arg Ala Tyr Leu His Thr Ala Cys His Pro Val Thr Gly Leu Ser
225 230 235 240
Pro Glu Tyr Ala Asn Tyr Asp Gly Ser Pro Ala Pro Val Gln Pro His
245 250 255
Gly Asp Phe Arg His Phe Tyr Ser Asp Ala Tyr Arg Val Ala Ala Asn
260 265 270
Ile Ala Leu Asp Trp Glu Trp Phe Gly Lys Asp Pro Trp Gln Val Glu
275 280 285
Gln Ser Asn Arg Ile Gln Ser Phe Phe Ser Ser Ile Asp Val Ala Glu
290 295 300
Tyr Arg Arg Tyr Thr Ile Glu Gly Glu Pro Phe Asp Glu Pro Ser Leu
305 310 315 320
His Pro Ile Gly Leu Leu Ala Thr Asn Ala Met Ala Ser Leu Ala Ala
325 330 335
Asp Gly Pro His Val Glu His Phe Val Arg Leu Phe Trp Asn Thr Pro
340 345 350
Leu Arg Gln Gly Glu Arg Arg Tyr Tyr Asp Asn Cys Leu Tyr Phe Phe
355 360 365
Ser Leu Leu Ala Leu Ser Gly Lys Tyr Arg Ile Tyr
370 375 380
<210> 19
<211> 371
<212> PRT
<213> Cronobacter sakazakii
<400> 19
Met Lys Asn Leu Cys Arg Trp Leu Ala Ala Gly Leu Leu Leu Ala Thr
1 5 10 15
Val Ser Ala His Ala Ala Cys Gly Trp Ala Asp Trp Asp Arg Phe Lys
20 25 30
Gly Gly Tyr Ile Ser Glu Glu Gly Arg Val Ile Asp Pro Ser Asp Pro
35 40 45
Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala
50 55 60
Leu Ala Ala Asn Asp Arg Glu Ala Phe Asp Asn Ile Leu Gln Trp Thr
65 70 75 80
Glu Asn Asn Leu Ala Gln Gly Ser Leu Lys Asn Thr Leu Pro Ala Trp
85 90 95
Leu Trp Gly Met Lys Gly Pro Asp Glu Trp Thr Val Leu Asp Thr Asn
100 105 110
Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala Trp Ser Leu Leu Glu Ala
115 120 125
Gly Arg Leu Trp Lys Glu Pro Arg Tyr Ser Asp Thr Gly Lys Ala Leu
130 135 140
Leu Asp Arg Ile Ala Lys Glu Glu Val Val Asn Val Pro Ala Leu Gly
145 150 155 160
Asp Met Leu Leu Pro Gly Lys Val Ser Phe Ala Glu Pro Thr Leu Trp
165 170 175
Arg Phe Asn Pro Ser Tyr Met Pro Pro Gln Leu Ala Arg Tyr Phe Thr
180 185 190
Arg Tyr Gly Lys Pro Trp Thr Thr Ile Arg Asp Thr Asn Leu Arg Leu
195 200 205
Leu Leu Glu Thr Ala Pro Gln Gly Phe Ser Pro Asp Trp Val Ser Tyr
210 215 220
Glu Ala Lys Lys Gly Trp Val Met Lys Pro Ala Ala Pro Gln Lys Pro
225 230 235 240
Leu Ile Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Val Gly Met
245 250 255
Met Asn Pro Ala Asp Glu Gln Gln Ala Pro Leu Leu Lys Lys Leu Ser
260 265 270
Pro Met Glu Thr Ala Thr Ile Lys Ala Gly Val Pro Pro Glu Lys Val
275 280 285
Arg Val Ile Asp Gly Lys Thr Gln Gly Asn Gly Pro Val Gly Phe Ser
290 295 300
Ala Ala Leu Leu Pro Phe Leu Gln Asn Arg Asp Ile Gln Ala Leu Gln
305 310 315 320
Arg Gln Arg Val Ala Asp Asn Tyr Pro Gln Gln Asp Ala Tyr Phe Ser
325 330 335
Tyr Val Leu Thr Leu Phe Gly Gln Gly Trp Asp Glu His Arg Phe Arg
340 345 350
Phe Thr Val Asp Gly Glu Leu Gln Pro Ala Trp Gly Glu Ser Cys Thr
355 360 365
Ser Ser Arg
370
<210> 20
<211> 407
<212> PRT
<213> 越南伯克霍尔德氏菌
<400> 20
Met Val Trp Ala Tyr Ala Thr Arg Arg Ala Ala Arg Arg Ala Gly Ala
1 5 10 15
Ala Leu Ala Leu Ala Ala Ala Ala Ala Ala Gly Pro Leu His Ala Ala
20 25 30
Gln Asp Gly Ala Gly Ala Ala Ala Gly Ser Ala Gly Ala Ala Gly Ala
35 40 45
Gln Cys Gly Gly Ala Ala Trp Pro Arg Trp Asp Ala Phe Lys Arg Asp
50 55 60
Phe Val Ser Ala Asp Gly Arg Val Ile Asp Val Gly Ser Asp Asp Ser
65 70 75 80
Arg Thr Val Ser Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val
85 90 95
Ala Asn Asp Arg Arg Thr Phe Asp Thr Ile Leu Ala Trp Thr Glu Asn
100 105 110
Asn Leu Ala Gln Gly Asp Leu Ser Ala His Leu Pro Ala Trp Leu Trp
115 120 125
Gly Arg Ala Pro Asp Gly Ala Trp Arg Val Leu Asp Ala Asn Ala Ala
130 135 140
Ser Asp Ala Asp Leu Trp Ile Ala Tyr Thr Leu Val Glu Ala Gly Arg
145 150 155 160
Leu Trp His Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu Ala Lys
165 170 175
Arg Val Leu Asp Thr Glu Thr Ala Ser Val Pro Gly Leu Gly Leu Thr
180 185 190
Leu Leu Pro Gly Pro Thr Gly Phe Lys Leu Ala Asn Gly Gln Trp Arg
195 200 205
Val Asn Pro Ser Tyr Ser Pro Pro Gln Val Ile Arg Gly Leu Ala Ala
210 215 220
Arg Leu Pro Asp Glu Arg Arg Trp Ala Ala Leu Met Thr Ser Thr Gly
225 230 235 240
Arg Val Leu Leu Asp Thr Ala Pro Lys Gly Phe Ala Pro Asp Trp Ala
245 250 255
Leu Tyr Arg Ala Gly Ala Gly Phe Gly Pro Asp Pro Gln Thr His Ala
260 265 270
Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Leu
275 280 285
Asp Arg Ser Asp Pro Leu Ala Ala Pro Leu Leu Ala Arg Phe Ala Pro
290 295 300
Phe Ala Asp His Ile Ala Ala His Gly Ala Pro Pro Glu Arg Val Asp
305 310 315 320
Thr Thr Thr Gly Val Ala Ala Pro Asn Asp Gly Asn Gly Gly Phe Ser
325 330 335
Ala Ala Ala Val Pro Phe Leu Ala Ala Arg Gly Gln Gln Ala Leu Ala
340 345 350
Asp Ala Gln Ala Ala Arg Val Asp Ala Leu Ala Arg Gln Ser Pro Pro
355 360 365
Gly Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu Gly Trp Arg Asp
370 375 380
Gly Arg Tyr Arg Phe Gly Ala Asp Gly Thr Leu Asp Ala Arg Trp Glu
385 390 395 400
Gly Arg Pro Cys Ala Ala Arg
405
<210> 21
<211> 337
<212> PRT
<213> Polynucleobacter necessarius
<400> 21
Met Thr Phe Ser Pro Leu Leu Arg Ser Trp Leu Val Thr Ile Ala Leu
1 5 10 15
Gly Val Ile Ser Ser Asn Ala Ile Cys Ala Asp Gln Asp Trp Gln Gln
20 25 30
Phe Lys Asn Ala Tyr Ile Gln Asn Gly Arg Val Ile Asp Ser Gly Gln
35 40 45
Asp Gly Met Ser His Thr Glu Gly Gln Gly Val Ala Met Leu Leu Ala
50 55 60
Val Lys Asn Asn Asp Pro Gln Thr Phe Glu Leu Val Trp Asn Trp Thr
65 70 75 80
Gln His Asn Leu Gln Val Arg Glu Asp Lys Leu Leu Ala Trp Ser Trp
85 90 95
Ser Pro Thr Gln Gly Val Met Asp Thr Asn Asn Ala Ser Asp Gly Asp
100 105 110
Leu Phe Ile Ala Trp Ala Leu Ser Gln Ala Tyr Thr Arg Trp Gln Glu
115 120 125
Pro Arg Tyr Leu Phe Tyr Ala Leu Gln Ile Ser Gln Ser Ile Arg Glu
130 135 140
Lys Leu Ile Arg Lys Thr Asn Phe Gly Thr Val Ile Leu Pro Gly Ala
145 150 155 160
Phe Gly Phe Glu Lys Pro Glu Gly Leu Lys Leu Asn Leu Ser Tyr Trp
165 170 175
Val Phe Pro Ala Ile Thr Glu Leu Ala Val Leu Asp Pro Ala Pro Glu
180 185 190
Trp Asn Glu Leu Gln Leu Thr Gly Leu Arg Leu Leu Glu Gln Ala Gln
195 200 205
Tyr Gly Lys Trp Lys Leu Pro Pro Asp Trp Leu Met Tyr Lys Asp Gly
210 215 220
Ile Ser Thr Pro Thr Asp Gly Asn Arg Phe Gly Tyr Asp Ala Val Arg
225 230 235 240
Ile Pro Leu Tyr Leu Ile Trp Gly Asn Gln Ala Gly Asn Ser Asn Met
245 250 255
Lys Pro Phe Gln Ser Phe Trp Ser Ser Phe Gln Gly Gln Glu Phe Leu
260 265 270
Pro Ala Trp Val Asp Leu Lys Thr Gly Asp Ile Gly Thr Tyr Asn Ala
275 280 285
Ser Leu Gly Phe His Ser Ile Ala Ala Leu Thr Leu Ser Tyr Pro Gln
290 295 300
Ile Asp Leu Ala Gln Leu Pro Asn Phe Asp Pro Thr Glu Gly Tyr Tyr
305 310 315 320
Ser Ser Met Leu Tyr Leu Phe Thr Lys Ser Ala Val Glu Asp Leu Lys
325 330 335
Lys
<210> 22
<211> 360
<212> PRT
<213> 肠杆菌属菌种638
<400> 22
Met Val Ala Leu Val Leu Ala Ala Ala Asn Ala Arg Ala Ala Cys Ser
1 5 10 15
Trp Pro Ala Trp Glu Gln Phe Lys Gln Asp Tyr Ile Ser Asp Gly Gly
20 25 30
Arg Val Ile Asp Pro Ser Asp Ala Arg Lys Ile Ser Thr Ser Glu Gly
35 40 45
Gln Ser Tyr Ala Leu Phe Phe Ala Leu Ala Ala Asn Asp Arg Lys Ala
50 55 60
Phe Asp Leu Leu Leu Thr Trp Thr Ser Asp Asn Leu Ala Gln Gly Ser
65 70 75 80
Leu Ser Gln His Leu Pro Ala Trp Leu Trp Gly Lys Lys Asp Ala Asp
85 90 95
Thr Trp Ala Val Ile Asp Lys Asn Ser Ala Ser Asp Ala Asp Ile Trp
100 105 110
Ile Ala Trp Ser Leu Leu Glu Ala Gly Arg Leu Trp Lys Ala Pro Gln
115 120 125
Tyr Thr Ala Thr Gly Lys Ala Leu Leu Lys Arg Ile Ala Ser Glu Glu
130 135 140
Val Ile Lys Val Pro Gly Leu Gly Leu Met Leu Leu Pro Gly Asn Val
145 150 155 160
Gly Phe Thr Glu Glu Lys Ala Trp Arg Phe Asn Pro Ser Tyr Leu Pro
165 170 175
Pro Gln Leu Ala Asn Tyr Phe Thr Arg Phe Gly Ala Pro Trp Thr Thr
180 185 190
Leu Arg Glu Thr Asn Leu Arg Leu Leu Leu Glu Thr Ala Pro Lys Gly
195 200 205
Phe Ala Pro Asn Trp Val Gln Tyr Gln Gln Lys Lys Gly Trp Gln Leu
210 215 220
Gln Pro Glu Lys Thr Phe Ile Gly Ser Tyr Asp Ala Ile Arg Val Tyr
225 230 235 240
Leu Trp Thr Gly Met Met His Asp Arg Asp Pro Gln Lys Ala Arg Leu
245 250 255
Leu Ala Arg Phe Lys Pro Met Ala Thr Leu Thr Thr Lys Asn Gly Val
260 265 270
Pro Pro Glu Lys Val Asp Val Ala Ser Gly Lys Pro Thr Gly Asp Gly
275 280 285
Pro Val Gly Phe Ser Ala Ser Leu Leu Pro Phe Leu Gln Asp Arg Asp
290 295 300
Ala Gln Ala Val Gln Arg Gln Arg Val Ala Asp His Phe Pro Gly Asn
305 310 315 320
Asp Ala Tyr Tyr Ser Tyr Val Leu Thr Leu Phe Gly Gln Gly Trp Asp
325 330 335
Gln His Arg Phe Arg Phe Thr Ala Lys Gly Glu Leu His Pro Asp Trp
340 345 350
Gly Gln Glu Cys Ala Ser Ser His
355 360
<210> 23
<211> 330
<212> PRT
<213> 肠杆菌属菌种638
<400> 23
Met Arg Lys Pro Ala Cys Ala Ala Leu Ala Val Met Met Thr Met Ile
1 5 10 15
Phe Ser Pro Phe Ser Gln Ala Gly Gln Ala Trp Glu Ser Tyr Lys Ala
20 25 30
Arg Phe Leu Met Pro Asp Gly Arg Val Val Asp Thr Gly Asn Gly Asn
35 40 45
Val Ser His Thr Glu Gly Gln Gly Phe Ala Met Leu Met Ala Val Ala
50 55 60
Ser Asp Asp Lys Thr Ala Phe Asp Lys Leu Trp His Trp Thr Asp Ser
65 70 75 80
Thr Leu Lys Asn Lys Ser Asn Gly Leu Phe Tyr Trp Arg Tyr Asn Pro
85 90 95
Val Leu Pro Asp Pro Ile Pro Asp Lys Asn Asn Ala Ser Asp Gly Asp
100 105 110
Ala Leu Ile Ala Trp Ala Leu Leu Lys Ala Asp Ala Arg Trp His Asp
115 120 125
Ala Arg Tyr Ser Gln Ala Ser Asp Ala Ile Thr Lys Ser Leu Val Ala
130 135 140
Arg Thr Val Ile Arg Tyr Ala Gly Tyr Arg Val Met Leu Pro Gly Ala
145 150 155 160
Gln Gly Phe Asn Leu Asn Ser Glu Val Ile Leu Asn Pro Ser Tyr Phe
165 170 175
Ile Phe Pro Ala Trp Gln Ala Phe Ala Asp Arg Ser His Leu Gln Val
180 185 190
Trp Arg Glu Leu Ile Gln Asp Gly His Lys Val Leu Gly Lys Met Gly
195 200 205
Thr Gly Thr Ala Asn Leu Pro Thr Asp Trp Met Ser Leu Ala Ala Gly
210 215 220
Gly Lys Leu Thr Pro Ala Asn Ala Trp Pro Pro Arg Met Ser Tyr Asp
225 230 235 240
Ala Ile Arg Ile Pro Leu Tyr Ile His Trp Ser Ser Pro Gln Ser Pro
245 250 255
Ser Leu Thr Pro Trp Arg Ser Trp Phe Gly Gln Phe Ser Arg Glu Lys
260 265 270
Thr Pro Ala Trp Val Asn Val Thr Thr Asn Glu Tyr Ala Pro Tyr Met
275 280 285
Met Glu Gly Gly Leu Leu Ala Val Arg Asp Leu Thr Met Gly Gln Ala
290 295 300
Ser Gly Glu Pro Asp Ile Thr Ala Lys Asp Asp Tyr Tyr Ser Ala Ser
305 310 315 320
Leu Lys Met Leu Val Trp Leu Ala Asp Gln
325 330
<210> 24
<211> 356
<212> PRT
<213> 类球红细菌
<400> 24
Met Arg Arg Arg Thr Leu Leu Arg Leu Ala Ala Ala Thr Ala Leu Ala
1 5 10 15
Ser Pro Ala Gly Arg Leu Leu Ala Gln Glu Gly Gly Val Leu Pro Ser
20 25 30
Asp His Pro Leu Gln Thr Ala Trp Glu Ser Trp Lys Ala Ala Phe Leu
35 40 45
Leu Pro Ala Gly Arg Ile Val Asp Gly Pro Gln Gln Asn Ala Ser His
50 55 60
Ser Glu Gly Gln Gly Tyr Gly Ala Ala Leu Ala Ala Ile Phe Gly Asp
65 70 75 80
Glu Ala Ala Leu Arg Arg Ile Val Asp Trp Thr Glu Thr Asn Leu Ala
85 90 95
Arg Arg Asp Asp Asn Leu Leu Ser Trp Arg Trp Leu Pro Gly Val Pro
100 105 110
Leu Ala Val Pro Asp Glu Asn Asn Ala Thr Asp Gly Asp Leu Phe Tyr
115 120 125
Gly Trp Gly Leu Ala Leu Ala Ala Gln Arg Phe Gly Asn Ala Asp Leu
130 135 140
Ala Lys Arg Ala Thr Glu Ile Ala Arg Ala Ile Ala Leu His Cys Val
145 150 155 160
Arg Pro His Pro Asp Gly Ser Glu Arg Leu Val Leu Leu Pro Gly Ala
165 170 175
Thr Gly Phe Glu Thr Glu Glu Gly Leu Val Leu Asn Pro Ser Tyr Tyr
180 185 190
Met Pro Arg Ala Met Thr Glu Leu Ala Ala Phe Ser Gly Gln Glu Arg
195 200 205
Leu Ala Arg Cys Ala Gln Asp Gly Ala Leu Trp Ile Gly Gly Leu Gly
210 215 220
Leu Ala Pro Asp Trp Val Leu Val Thr Ser Thr Gly Asp Leu Pro Ala
225 230 235 240
Lys Gly Leu Ser Ala His Ser Gly Tyr Asp Ala Met Arg Val Pro Leu
245 250 255
Phe Leu Leu Trp Ser Gly Leu Thr Ala Asn Pro Ala Leu Arg Arg Phe
260 265 270
Ile Glu Val Gln Arg Glu Ala Glu Pro Gly Thr Gly Thr Pro Val Val
275 280 285
Phe Asp Arg Asp Thr Gly Ala Leu Leu Glu Arg Ser Ala Asp Pro Gly
290 295 300
Phe Ala Ser Val Pro Ala Leu Ala Asp Cys Ala Leu Ser Gly Arg Pro
305 310 315 320
Gly Ala Ala Ile Pro Pro Phe Asp Ala Arg Gln Pro Tyr Tyr Pro Ala
325 330 335
Thr Leu His Leu Met Thr Leu Val Ala Gln Val Glu Gly Phe Ser Ala
340 345 350
Cys Ala Pro Ile
355
<210> 25
<211> 362
<212> PRT
<213> 类球红细菌
<400> 25
Met Lys Arg Arg His Phe Met Ala Ser Leu Leu Gly Thr Leu Ala Ala
1 5 10 15
Gly Arg Ala Phe Gly Gly Phe Ala Gln Glu Gly Ala Ala Phe Val Leu
20 25 30
Pro Ala Asp His Pro Leu Gln Pro Ala Trp Gln Ala Trp Lys Ser Leu
35 40 45
Cys Leu Gln Glu Asp Gly Arg Ile Val Asp Ala Pro Gln Ser Gly Ala
50 55 60
Ser His Ser Glu Gly Gln Gly Tyr Gly Leu Arg Leu Ala Val Ala Phe
65 70 75 80
Gly Asp Glu Glu Ala Phe Arg Arg Ile Phe Glu Trp Ser Glu Ser His
85 90 95
Leu Ala Leu Arg Glu Asp Gly Leu Leu Ser Trp Arg Tyr Leu Pro Glu
100 105 110
Ala Gly Asp Pro Val Pro Asp Arg Asn Asn Ala Ser Asp Gly Asp Leu
115 120 125
Phe Tyr Gly Trp Ala Leu Ile Gln Gly Ala Met Ala Trp Asn Glu Pro
130 135 140
Leu Phe Ala Asp Arg Ala Arg Met Ile Gly Thr Ala Leu Ala Arg Ser
145 150 155 160
Cys Leu Ala Asp His Pro Asp Gly Ser Gly Arg Arg Tyr Leu Leu Pro
165 170 175
Ala Ala Tyr Gly Phe Thr Ser Asp Arg Ala Ile Thr Leu Asn Leu Ser
180 185 190
Tyr Cys Met Pro Arg Ala Met Arg Glu Leu Gly Ala Phe Ala Glu Ala
195 200 205
Pro Ala Leu Val Thr Ala Ala Gln Asp Gly Leu Asp Leu Met Asn Gly
210 215 220
Ile Ala Gly Ser Gly Leu Leu Pro Asp Trp Leu Gln Leu Thr Pro Glu
225 230 235 240
Gly Arg Thr Pro Ala Pro Gly Leu Pro Asp Gln Ser Gly Tyr Glu Ala
245 250 255
Leu Arg Ile Pro Leu Tyr Leu Cys Trp Ser Gly Met Thr Asp Thr Pro
260 265 270
Ala Gln Gln Arg Phe Arg Glu Met His Arg Gln Ala Ala Ala Arg Asp
275 280 285
Arg Gly Thr Ala Thr Val Phe Asp Pro Glu Thr Gly Arg Ile Arg Glu
290 295 300
Ser Ser Asp Glu Val Gly Tyr Arg Ala Leu Val Ala Leu Asn Asp Cys
305 310 315 320
Val Leu Ser Arg Thr Ala Gly Ser Ala Met Pro Asp Phe Asp Ala Ala
325 330 335
Gln Val Tyr Phe Pro Ala Thr Leu His Leu Met Ala Leu Val Ala Gln
340 345 350
Ala Glu Phe Phe Pro Lys Cys Leu Pro Val
355 360
<210> 26
<211> 430
<212> PRT
<213> Flavobacterium johnsoniae
<400> 26
Met Thr Val His His Asn Ile Thr Asn Gly Tyr Leu Ser Lys Ile His
1 5 10 15
Leu Leu Leu Leu Ile Phe Ala Leu Ile Val Pro Ala Phe Val Ile Ser
20 25 30
Gln Asn Lys Lys Lys Ile Asp Arg Glu Leu Lys Lys Pro Gln Tyr Arg
35 40 45
Asn Leu Phe Lys Glu Ala Gly Tyr Ser Gln Asp Asp Ile Asp Lys Lys
50 55 60
Leu Thr Lys Ala Tyr Tyr Asp Val Phe Glu Gly Pro Asp Lys Val Tyr
65 70 75 80
Phe Glu Glu Gly Asp Ser Leu Gly Tyr Val Ser Asp Val Lys Asn Lys
85 90 95
Asp Ala Arg Thr Glu Gly Met Ser Tyr Gly Met Met Val Ala Val Gln
100 105 110
Phe Asn Lys Lys Asp Val Phe Asp Arg Leu Trp Arg Trp Ser Val Lys
115 120 125
Tyr Met Gln His Gln Asp Gly Pro Arg Glu Gly Tyr Phe Ala Trp Ser
130 135 140
Val Asn Pro Gln Thr Lys Lys Gln Asn Ser Ala Gly Ser Ala Ser Asp
145 150 155 160
Gly Glu Leu Tyr Tyr Ile Thr Ser Leu Leu Phe Ala Ser Asn Lys Trp
165 170 175
Gly Asn Asp Thr Gly Ile Asn Tyr Tyr Lys Glu Ala Arg Arg Ile Leu
180 185 190
Asp Ala Met Trp Lys Lys Asp Gly Thr Gly Asn Ile Tyr Asn Ile Ile
195 200 205
Asn Thr Glu His Lys Gln Ile Ser Phe Val Pro Glu Gly Gly Gly Tyr
210 215 220
Asn Trp Thr Asp Pro Ser Tyr His Val Pro Ala Phe Tyr Glu Ile Trp
225 230 235 240
Ala Leu Tyr Ala Lys Asp Gly His Glu Gln Phe Tyr Lys Glu Cys Ala
245 250 255
Glu Val Ser Arg Lys Phe Leu His Lys Ala Cys His Pro Val Thr Gly
260 265 270
Leu Thr Ser Asp Tyr Thr Glu Phe Asn Gly Glu Pro His Pro Thr Pro
275 280 285
Trp Leu Pro Pro Gly Phe Arg Tyr Asp Ser Trp Arg Val Pro Met Asn
290 295 300
Ile Ala Met Asp Tyr Thr Trp Tyr Gly Lys Asp Lys Glu Trp Gln Glu
305 310 315 320
Asp Tyr Ala Lys Arg Phe Gln Asn Phe Leu Arg Ser Lys Gly Leu Glu
325 330 335
Thr Tyr Glu Asp Gln Phe Asn Leu Asp Gly Ser Thr Pro Glu Phe Ile
340 345 350
Leu Gln Ala Gly Pro Val Lys Lys Leu Arg His Ser Ile Gly Leu Val
355 360 365
Gly Thr Ala Ala Thr Ala Ser Leu Val Asn Lys Asp Lys Ala Ser Ile
370 375 380
Asp Phe Val His Ala Val Trp Asn Ala Lys Leu Glu Pro Tyr Glu Asp
385 390 395 400
Gly Tyr Phe Asp Pro Tyr Tyr Asp Gly Leu Met Tyr Leu Phe Ser Leu
405 410 415
Met His Leu Ser Gly Asn Tyr Gln Ile Ile Val Pro Lys Gly
420 425 430
<210> 27
<211> 465
<212> PRT
<213> Flavobacterium johnsoniae
<400> 27
Met Lys Asn Leu Leu Leu Ile Thr Val Ala Phe Leu Cys Leu Glu Ser
1 5 10 15
Asn Ala Gln Lys Gln Pro Phe Pro Ala Asn Val Val Phe Asn Asn Gly
20 25 30
Leu Met Pro Ser Ala Lys Asn Ser Gln Asp Ala Lys Asn Asn Tyr Asp
35 40 45
Thr Trp Lys Thr Asn Phe Val Glu Gly Cys Ser Asn Gly Arg Tyr Arg
50 55 60
Val Lys Phe Asp Ser Ser Trp Glu Thr Val Ser Glu Gly Ile Gly Tyr
65 70 75 80
Gly Met Leu Leu Ser Val Tyr Met Ala Asp Lys Thr Leu Phe Asp Gly
85 90 95
Leu Trp Leu Tyr Tyr Lys Asp Asn Val Asn Asn Asn Lys Val Met Asn
100 105 110
Trp Lys Ile Asn Gly Cys Ser Gly Thr Ile Gly Gln Asn Gly Ala Thr
115 120 125
Asp Ala Glu Leu Asp Ala Ala Phe Ala Leu Ile Val Ala Asp Tyr Gln
130 135 140
Trp Ala Ser Ala Gly Thr Ile His Tyr Lys Asn Asp Ala Thr Ala Leu
145 150 155 160
Ile Ser Ala Ile Lys Asn His Glu Val Glu Ala Asn Thr Tyr Val Leu
165 170 175
Lys Pro Gly Asp Gln Phe Gly Gly Ser Gln Ile Thr Asn Pro Ser Tyr
180 185 190
Phe Ser Pro Ala Tyr Tyr Arg Val Phe Gly Thr Phe Thr Asn Asp Thr
195 200 205
Ala Phe Trp Asn Gln Ile Ala Ala Lys Ser Tyr Thr Ile Ile Asn Asn
210 215 220
Asn Leu Thr Gln Asn Asn Ala Val Gly Gly Leu Val Ser Asp Trp Cys
225 230 235 240
Glu Ala Ser Gly Ala Tyr Ser Ser Gln Ala Gly Gly Tyr Ala Asn Gly
245 250 255
Gly Lys Met Tyr Thr Tyr Asp Ala Ala Arg Thr Pro Trp Arg Ile Ala
260 265 270
Val Asp Tyr Leu Trp His Gly Asn Ala Asp Ala Lys Ala Tyr Ala Lys
275 280 285
Lys Ser Ser Asp Phe Val Arg Val Asn Leu Gly Gly Ser Leu Asn Ile
290 295 300
Lys Asp Gly Tyr Asn Gln Asn Gly Ser Val Ser Gly Gln Trp His Asn
305 310 315 320
Ala Thr Phe Val Gly Ala Phe Ala Cys Ala Ala Met Ala Gly Glu Asn
325 330 335
Gln Asn His Leu Asp Ala Ser Tyr Thr Asp Leu Lys Asn Leu Asn Glu
340 345 350
Pro Asn Ser Tyr Phe Asn His Thr Leu Lys Thr Leu Tyr Ser Phe Leu
355 360 365
Leu Thr Gly Asn Phe Tyr Leu Pro Leu Asn Ala Asn Leu Ser Asn Asp
370 375 380
Thr Phe Asp Ile Glu Lys Ser Thr Val Thr Leu Tyr Pro Asn Pro Ser
385 390 395 400
Pro Asp Arg Ile Thr Val Asn Ala Pro Gln Gln Ser Thr Ile Ser Val
405 410 415
Ile Ser Pro Ser Gly Ser Val Ile Tyr Gln Lys Lys Thr Thr Ser Glu
420 425 430
Asn Thr Glu Ile Asn Leu Gly Ser Gln Ser Ser Gly Ile Tyr Phe Val
435 440 445
Lys Ile Ser Asn Asp Asp Phe Lys Ser Ile Thr Lys Lys Val Ile Leu
450 455 460
Lys
465
<210> 28
<211> 376
<212> PRT
<213> 隐藏嗜酸菌
<400> 28
Met Thr Asp Met Ala Arg Arg Asn Phe Leu Gly Leu Leu Gly Ala Ala
1 5 10 15
Ala Leu Thr Gly Gly Pro Val Met Met Ala Arg Glu Leu Trp Gln Ser
20 25 30
Ser Trp Arg Gly Tyr Arg His Gly Phe Ile Asp Gly Gln Gly Arg Val
35 40 45
Ile Asp Tyr Ser Ala Asn Lys Gly Phe Ser Thr Ser Glu Gly Gln Ser
50 55 60
Tyr Gly Met Phe Leu Ser Leu Val Ala Gly Asp Arg Ala Thr Phe Arg
65 70 75 80
Arg Ile Leu Asn Trp Thr Asn Thr Asn Met Ala Gly Gly Arg Leu Gly
85 90 95
Glu Val Leu Ala Ala Trp Lys Trp Gly Leu His Gly Gly Lys Trp Gly
100 105 110
Val Ile Gly Ala Asn Ser Ala Ala Asp Ala Asp Ala Trp Met Ala Tyr
115 120 125
Ser Leu Leu Glu Ala Ala Arg Ile Trp Lys Asp His Asn Leu Gly Ala
130 135 140
Glu Gly His Lys Leu Ala Thr Arg Ile Ala Asp Asp Glu Ser Val Ala
145 150 155 160
Ile Asn Gly Phe Gly Arg Val Leu Ile Pro Gly Ala Ser Asp Phe Pro
165 170 175
Asp Thr Pro Pro Val Ile Val Asp Pro Ser Tyr Thr Pro Leu Phe Leu
180 185 190
Ala Arg Gly Ile Ala Arg Ala Thr Asn Leu Pro Lys Trp Gln Ala Ile
195 200 205
Ala Ala Thr Leu Pro Arg Leu Met Thr Thr Ile Cys Arg Asn Gly Phe
210 215 220
Ala Pro Asp Trp Ala Trp Ala Pro Gln Ala Pro Ala Ser Pro Pro Ala
225 230 235 240
Gly Leu Pro Glu Thr Gly Thr Gly Ser Phe Asp Ala Ile Arg Cys Tyr
245 250 255
Leu Trp Ala Gly Leu Thr Ala Pro Glu Thr Glu Gly Ser Ala Thr Val
260 265 270
Leu Ala Ser Leu Lys Gly Met Ala Arg Tyr Leu Ala Thr His Arg Ala
275 280 285
Pro Pro Gln Ser Val Asp Leu Ala Ser Gln Ala Thr His Gly Thr Gly
290 295 300
Gly Ile Gly Phe Ser Ala Ala Leu Leu Pro Tyr Leu Ala Ala Leu Gly
305 310 315 320
Arg His Arg Leu Leu His Gln Gln Leu Gly Arg Val Leu Ala Gln Arg
325 330 335
Glu Thr Ser Gly Leu Phe Gly Gln Pro Ala Asp Tyr Tyr Ser Glu Asn
340 345 350
Leu Ile Leu Phe Gly Leu Gly Gly Leu Ser Gly Ser Ile Arg Phe Asp
355 360 365
Lys Gln Gly Gly Leu Ile Thr Ser
370 375
<210> 29
<211> 392
<212> PRT
<213> 费氏弧菌
<400> 29
Met Lys Met Gln Leu Thr Val Leu Ser Leu Leu Ile Gly Thr Leu Thr
1 5 10 15
Cys Ser Ser Ser Ala Ile Ala Gln Asp Glu Asn Tyr Thr Thr Pro Ile
20 25 30
Val Ser Ser Ala Ala Leu Thr Lys Glu Asn Gln Cys Glu Trp Gly Gln
35 40 45
Trp Glu Ser Phe Lys Arg His Tyr Ile Glu Asn Gly Arg Val Val Asp
50 55 60
Asn Ser Asp Pro Arg Leu Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala
65 70 75 80
Leu Phe Phe Ala Leu Ile Ala Asn Asp Lys Lys Thr Phe Asp Glu Leu
85 90 95
Leu Gly Trp Thr Glu Leu His Leu Ala Gly Gly Asp Leu Thr Ala Gln
100 105 110
Leu Pro Ala Trp Leu Trp Gly Thr Gln Pro Asp Gly Ser Gln Gly Ile
115 120 125
Leu Asp Ser Asn Ser Ala Ala Asp Ser Asp Leu Trp Ile Ala Tyr Ser
130 135 140
Leu Leu Glu Ala Gly Arg Leu Trp Asp Asn His Tyr Tyr Gln Ser Leu
145 150 155 160
Gly His Leu Leu Ala Ser Arg Ile Leu Arg Asp Glu Thr Ile Lys Val
165 170 175
Ser Gly Leu Gly Thr Val Leu Leu Pro Gly Lys Val Gly Phe Val Leu
180 185 190
Gly Lys Asn His Val Arg Leu Asn Pro Ser Tyr Val Pro Leu Gln Leu
195 200 205
Leu Thr Arg Met Asn Thr Val Phe Pro Ser Tyr Gln Trp Glu Glu Ile
210 215 220
Tyr Gln Ser Ser Ala Lys Leu Leu Lys Glu Thr Met Pro Lys Gly Tyr
225 230 235 240
Ser Pro Asp Trp Val Glu Trp Asp Lys Thr Gln Phe Lys Lys Asp Ser
245 250 255
Lys Ala Gln Ser Val Gly Ser Tyr Asn Ala Ile Arg Val Tyr Leu Trp
260 265 270
Ala Gly Met Leu Pro Asp Ser Asp Pro Asn Lys Ala Leu Leu Leu Gly
275 280 285
Lys Met Lys Pro Leu Leu Arg Val Ile Glu Arg Asn Lys Gly Met Pro
290 295 300
Glu Thr Ile Asn Val Leu Thr Gly Lys Gly Lys Asn Gln Gly Gly Val
305 310 315 320
Gly Met Asn Ala Ala Ile Leu Pro Leu Leu Ser Ser Leu Asp Ser Asn
325 330 335
Thr Asn Val Ala Glu Tyr Glu Lys Lys Ile Gln Ala Glu Leu Pro Lys
340 345 350
Ile Glu Ser Asp Tyr Tyr Tyr Asn Ser Val Leu Thr Leu Phe Gly Leu
355 360 365
Gly Trp Tyr Gln Asp Leu Tyr Ser Phe Asn Asp Asp Gly Ser Val Thr
370 375 380
Ser Lys Trp Val Asn Val Cys Gln
385 390
<210> 30
<211> 369
<212> PRT
<213> Vibrionales细菌SWAT-3
<400> 30
Met Lys Lys Leu Ile Leu Leu Leu Ser Leu Leu Leu Ser Pro Gln Leu
1 5 10 15
Met Ala Ala Thr Cys Glu Trp Pro Leu Trp Ser Asn Phe Lys Ser Ile
20 25 30
Tyr Ile Glu Asn Gly Arg Val Ile Asp Gly Ser Asp Glu Arg Leu Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala
50 55 60
Asn Asp Gln Gln Ala Phe Asp Gln Val Leu Lys Trp Thr Gln Ala Asn
65 70 75 80
Leu Ala Gly Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Arg Leu Asp Gly Gln Phe Gly Ile Leu Asp Thr Asn Pro Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ala Leu Gly Glu Ala Gly Arg Leu
115 120 125
Trp Asn Asn Tyr Tyr Tyr Gln Ser Leu Gly His Leu Leu Ala Ala Arg
130 135 140
Ile Leu Arg Glu Glu Ser Val His Ile Ala Gly Val Gly Thr Thr Leu
145 150 155 160
Leu Pro Ala Pro Lys Gly Phe Asp Leu Gly Lys Gly Gly Tyr Arg Leu
165 170 175
Asn Pro Ser Tyr Val Pro Leu Gln Leu Ile Ala Arg Met Lys Glu Leu
180 185 190
Tyr Pro Gln Tyr Ser Trp Asp Ser Met Tyr Gln Ser Ser Thr Leu Ile
195 200 205
Leu Ser Gln Thr Leu Thr Ala Gly Phe Ser Pro Asp Trp Val Thr Leu
210 215 220
Asn Gly Lys Arg Phe Asn Val Asp Lys Val Thr Gly Pro Ile Gly Ser
225 230 235 240
Tyr Asn Ala Ile Arg Thr Tyr Leu Trp Val Gly Met Leu Asn Glu Lys
245 250 255
Asn Lys Glu Lys Glu Gln Leu Val Lys Lys Met Glu Pro Phe Ala Thr
260 265 270
Ala Ile Glu Arg Leu Gly Ala Pro Pro Arg Glu Val Asp Thr Glu Thr
275 280 285
Gly Lys Tyr Ser Gln Ser Gly Ser Ala Gly Phe Ser Ala Ala Ala Leu
290 295 300
Pro Leu Leu Thr Ser Met Asp Tyr Ser Asn Leu Leu Glu Ala Gln Ala
305 310 315 320
Thr Arg Ala Gln Asn Glu Leu Val Thr Ser Arg Asn Asp His Tyr Tyr
325 330 335
Asp Asn Val Leu Ser Leu Phe Gly Leu Gly Trp His Asn Glu Arg Phe
340 345 350
Arg Phe Gly Glu His Gly Glu Leu Gln Pro Thr Trp Gly Lys Gln Cys
355 360 365
Gln
<210> 31
<211> 367
<212> PRT
<213> 恶臭假单胞菌
<400> 31
Met Leu Val Gly Leu Val Ala Leu Gly Leu Pro Val Phe Ala Asn Ala
1 5 10 15
Gly Ser Val Cys Pro Trp Pro Ala Trp Glu Arg Phe Lys Ala Glu Leu
20 25 30
Val Ser Val Asp Gly Arg Val Ile Asp Pro Ser Asp Glu Arg Leu Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Gly
50 55 60
Asn Asp Arg Gln Thr Phe Ala Gln Leu Leu Arg Trp Thr Ser Asn Asn
65 70 75 80
Leu Ala Glu Gly Asp Leu Ala Arg His Leu Pro Ala Trp Leu Trp Gly
85 90 95
Arg Asp Gly Gln Gln Gln Trp Gln Val Leu Asp Ala Asn Asn Ala Ser
100 105 110
Asp Ala Asp Leu Trp Ile Ala Tyr Ser Leu Leu Glu Ala Gly Arg Leu
115 120 125
Trp Asp Gln Pro Ala Tyr Thr Gln Leu Gly Gln His Leu Leu Trp Arg
130 135 140
Ile Ala Ala Gln Thr Val Arg Lys Leu Pro Gly Leu Gly Val Met Leu
145 150 155 160
Leu Pro Gly Asp Tyr Gly Phe Glu Asp Ala Gln Gly Thr Arg Leu Asn
165 170 175
Pro Ser Tyr Leu Pro Leu Gln Leu Leu Asp Arg Phe Ser Glu Val Asp
180 185 190
Pro Leu Trp Gly Glu Leu Ala Ala Asn Thr Arg Arg Leu Trp Leu Ala
195 200 205
Ser Ser Pro Lys Gly Phe Ala Pro Asp Trp Leu Leu Trp Thr Pro Ala
210 215 220
Gly Lys Pro Ala Ala Asp Thr Lys His Gly Asn Ala Gly Asp Tyr Asp
225 230 235 240
Ala Ile Arg Val Tyr Leu Trp Val Gly Met Leu Ala Glu Gly Ala Ala
245 250 255
Gln Arg Arg Glu Leu Val Ala His Tyr Ala Pro Met Ala Ala Leu Thr
260 265 270
Gln Arg Gln Gly Leu Pro Pro Glu His Leu Asp Ala Arg Ser Gly Glu
275 280 285
Ala Arg Gly His Gly Pro Ala Gly Phe Ser Ala Ala Leu Leu Pro Leu
290 295 300
Leu Ala Ala Ser Pro Glu His Val Ala Gly Leu Ala Ala Gln Arg Gln
305 310 315 320
Arg Leu Arg Glu Gln Pro Val Glu Ala Lys Ala Tyr Tyr Ser Gln Val
325 330 335
Leu Ala Leu Phe Gly Gln Gly Phe Asp Glu Ala Arg Tyr Arg Phe Asp
340 345 350
Pro His Gly Arg Leu Leu Pro Ala Trp Ser Ala Pro Cys Ser Glu
355 360 365
<210> 32
<211> 369
<212> PRT
<213> 哈氏弧菌
<400> 32
Met Lys Lys Leu Ile Ala Leu Met Ile Leu Met Ala Ser Pro Gln Leu
1 5 10 15
Trp Ala Asn Thr Cys Asp Trp Pro Gln Trp Asp Thr Phe Lys Ser Val
20 25 30
Tyr Ile Glu Gln Gly Arg Val Val Asp Gly Ser Asp Glu Arg Met Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala
50 55 60
Asn Asp Arg Lys Ala Phe Ala Glu Val Leu Lys Trp Thr Gln Ile His
65 70 75 80
Leu Ala Gly Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Arg Lys Ala Asn Gly Ser Phe Gly Val Leu Asp Thr Asn Ala Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ala Leu Val Glu Ala Gly Arg Leu
115 120 125
Trp Asp Asn Tyr Tyr Tyr Gln Ser Leu Gly His Leu Leu Ala Ser Arg
130 135 140
Ile Leu Arg Glu Glu Thr Ala Ser Ile Asp Gly Val Gly Ser Val Leu
145 150 155 160
Leu Pro Ala Pro Thr Gly Phe Glu Phe Gly Asp Arg Tyr Arg Val Asn
165 170 175
Pro Ser Tyr Val Pro Leu Gln Leu Ile Ala Arg Met Glu Ala Leu Tyr
180 185 190
Pro Gln Tyr Pro Trp Asp Gly Met Tyr Lys Ala Ser Ala Gln Met Leu
195 200 205
Thr Gln Thr Met Pro Lys Gly Phe Ser Pro Asp Trp Ala Thr Leu Ser
210 215 220
Gln Gly Gln Tyr Ser Ala Asp Ser Val Thr Gly Pro Ile Gly Ser Tyr
225 230 235 240
Asn Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Asp Asp Gln Val
245 250 255
Ala Glu Lys Ala Ser Leu Ile Gln Gln Met Gln Pro Phe Val Ala Ala
260 265 270
Thr Lys Ala Leu Gly Ala Pro Pro Arg Glu Val Asn Thr Glu Thr Gly
275 280 285
Glu Tyr Thr Gln Val Gly Ser Ala Gly Phe Ser Ala Ala Ser Leu Pro
290 295 300
Leu Leu Ala Ala Ser Gly Glu Ser Glu Leu Leu Lys Ala Gln Ala Ser
305 310 315 320
Arg Ala Gln Gln Glu Leu Ile Ser Asp Arg Asn Asp His Tyr Tyr Asp
325 330 335
Asn Val Leu Ser Leu Phe Gly Leu Gly Trp His Gln Ala Arg Tyr Arg
340 345 350
Phe Gly Lys Gln Gly Glu Leu Leu Pro Ala Trp Ser Glu Gln Cys Gln
355 360 365
Glu
<210> 33
<211> 452
<212> PRT
<213> Plesiocystis pacifica
<400> 33
Met Thr Ala Glu Asp Leu Glu Ala Gly Thr Glu Leu Gly Gly Ser Asp
1 5 10 15
Asp Val Gly Ser Thr Asp Gly Gly Ser Thr Asp Gly Gly Ser Thr Gly
20 25 30
Gly Ser Thr Gly Gly Thr Thr Gly Gly Gly Asp Thr Thr Ser Gly Gly
35 40 45
Thr Glu Gly Ser Gly Asp Gly Ala Cys Ser Gly Ser Trp Asp Thr Ala
50 55 60
Gln Pro Phe Gly Ser His Pro Phe Gly Tyr Ala Pro Gly Thr Ile Leu
65 70 75 80
Pro Asn His Leu Glu Gln Gly Ala Leu Asp Gln Gln Val Ala Gln Phe
85 90 95
Tyr Ala Gly Trp Lys Gln Arg Tyr Leu Ser Gly Gly Cys Gly Thr Gly
100 105 110
Arg Tyr Tyr Val Asp Ile Gln Thr Ala Asp Arg Leu Thr Val Ser Glu
115 120 125
Ala His Gly Tyr Gly Met Leu Ile Ser Ala Tyr Met Ala Gly His Asp
130 135 140
Pro Asp Ala Lys Ala Ile Phe Asp Gly Met Tyr Asp Tyr Phe Arg Asp
145 150 155 160
His Pro Ser Gln Gly Asn Ser Gln Leu Met Ala Trp Ala Gln Asp Gly
165 170 175
Val Cys Glu Asn Thr Asp Gly Ala Thr Ser Ala Thr Asp Gly Asp Leu
180 185 190
Asp Ile Ala Tyr Ala Leu Leu Leu Ala Asn Lys Gln Trp Gly Ser Gly
195 200 205
Gly Ala Ile Asp Tyr Ala Gly Glu Ala Ala Ala Ile Leu Gly Ala Ile
210 215 220
Asp Gln Ala Glu Val Asp Asp Ser Gly Ser Tyr Val Arg Leu Gly Asp
225 230 235 240
Trp Thr His Pro Ser Asn Ala Thr Tyr Tyr Asp Ala Thr Arg Ser Ser
245 250 255
Asp Phe Met Pro Ala His Phe Ala Thr Phe Ala Thr Phe Ile Gly Gly
260 265 270
Arg Trp Asp Gly Val Arg Asn Ala Thr Tyr Asp Leu Ile Ala Thr Val
275 280 285
Gln Ala Thr Tyr Ser Gln Gly Ser Gly Leu Leu Pro Asp Phe Ile Gln
290 295 300
His Pro Thr Ser Gly Ser Pro Ser Pro Val Ser Pro Asn Trp Leu Glu
305 310 315 320
Gly Pro Tyr Asp Gly Glu Tyr Tyr Phe Asn Ala Cys Arg Asn Pro Trp
325 330 335
Arg Ile Gly Val Asp Tyr Leu Leu Asn Gly Asp Pro Arg Ala Gln Ala
340 345 350
Ala Val Thr Gln Met Asn Gly Trp Val Gln Ser Ala Thr Gly Gly Ser
355 360 365
Pro Gly Asn Ile Arg Ala Gly Tyr Trp Leu Asp Gly Asp Ala Leu Pro
370 375 380
Thr Gly Asp Tyr Phe Glu Leu Ala Phe Ala Ala Pro Phe Gly Val Ala
385 390 395 400
Ala Met Ala Asp Pro Gly Ser Gln Ala Trp Leu Asn Asn Leu Trp Asp
405 410 415
Thr Leu Ile Ala Ala Asp Gly His Thr Tyr Tyr Gly Asp Ser Ile Thr
420 425 430
Met Leu Ser Leu Leu Ala Met Ser Gly Asn Trp Trp Ala Pro Glu Asp
435 440 445
Pro Pro Cys Gln
450
<210> 34
<211> 383
<212> PRT
<213> Limnobacter
<400> 34
Met Ala Leu Trp Arg Asn Cys Val Gln Arg Leu Gly Gln Val Ala Val
1 5 10 15
Leu Leu Ala Ala Thr Ala Ala Cys Ser Ala Thr Pro Ala Gln Ala Trp
20 25 30
Pro Ala Trp Asp Gly Phe Lys Ser Ala Phe Val Ser Asp Asp Gly Arg
35 40 45
Val Ile Asp Arg Ser Gln Glu Asp Leu Arg Thr Val Ser Glu Gly Gln
50 55 60
Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala Gln Asp Lys Lys Ala Phe
65 70 75 80
Asp Ala Val Leu Gln Trp Thr Glu Asn Asn Leu Ser Ala Gly Asp Leu
85 90 95
Gly Lys Gln Leu Pro Ala Trp Ile Trp Gly Lys Lys Gly Glu Ser Trp
100 105 110
Gly Val Ile Asp Ala Asn Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala
115 120 125
Tyr Ser Leu Leu Glu Ala Ser Arg Val Trp Cys Asn Pro Gly Tyr Ala
130 135 140
Asp Lys Ala Arg Ala Leu Gly Asp Leu Ile Leu Asn Gln Glu Ser Met
145 150 155 160
Glu Val Ser Gly Leu Gly Leu Ser Ile Leu Pro Gly His Thr Gly Phe
165 170 175
Val Leu Asp Asn Gly Ala Val Lys Leu Asn Pro Ser Tyr Leu Pro Pro
180 185 190
Phe Met Met Ala Arg Phe Ala Asn Ala Trp Ala Asp Asp Thr Arg Trp
195 200 205
Ala Gln Val Tyr Leu Ala Ser Gln Lys Leu Leu Leu Asp Thr Gly Arg
210 215 220
Thr Gly Gln Tyr Pro Asp Trp Val Leu Tyr Asn Asn Gly Glu Met Ser
225 230 235 240
Leu Pro Glu Asp Glu Gln Arg Gly Asp Tyr Asp Ala Ile Arg Thr Tyr
245 250 255
Leu Trp Ile Ala Met Ser Ser Glu Ser Asp Pro Thr Thr Ala Pro Leu
260 265 270
Leu Arg Gln Leu Ser Pro Leu Thr Ala Leu Leu Leu Lys Arg Gln Asn
275 280 285
Met Pro Glu Trp Phe Glu Pro Gln Ser Gly Lys Phe Ser Ala Thr Arg
290 295 300
Gly Pro Ala Gly Phe Gln Ala Ala Met Ala Pro Leu Leu Gln Thr Met
305 310 315 320
Gly Met Pro Glu Leu Ala Lys Lys Phe His Ala Gln Ser Leu Lys Thr
325 330 335
Gln Ser Lys Glu Ser Trp Leu Lys Tyr Gly Tyr Tyr Asn Gly Ala Leu
340 345 350
Ser Leu Phe Ala Gln Gly Tyr Ile Asp Lys Phe Tyr Arg Phe Asn Ser
355 360 365
Leu Gly Glu Leu Leu Pro Arg Gly Lys Glu Val Lys Ser Cys Gly
370 375 380
<210> 35
<211> 380
<212> PRT
<213> Limnobacter
<400> 35
Met Ile Leu Gly Phe Leu Asn Val Leu Phe Val Ala Gly Phe Val Leu
1 5 10 15
Ile Ala Asn Pro Gln Gln Ala Leu Ala Ser Ser Gln Cys Thr Lys Pro
20 25 30
Trp Pro Glu Leu Lys Leu Phe Glu Ser Lys His Val Gln Ala Asp Gly
35 40 45
Arg Val Val Asp Phe Ser Thr Pro Glu Gln Ile Thr Thr Ser Glu Gly
50 55 60
Gln Ser Tyr Ala Met Phe Phe Ala Leu Val Asp Asn Asp Gln Val Arg
65 70 75 80
Phe Lys Gln Leu Leu Asp Trp Thr Glu Arg Asn Leu Ala Pro Gly Gly
85 90 95
Val Ala Gln Ser Leu Pro Ala Trp Lys Trp Gly Arg Val Arg Ala Asn
100 105 110
Gln Phe Gly Met Leu Asp Ala Asn Ser Ala Ser Asp Ser Asp Leu Trp
115 120 125
Ile Ala Tyr Asn Leu Leu Glu Ala Ala Arg Leu Trp Asn Met Pro Glu
130 135 140
Tyr Lys Glu Lys Gly Leu Ala Leu Met Gln Leu Ile Arg Asp Asn Glu
145 150 155 160
Ala Gly Asp Val Pro Gly Tyr Gly Arg Met Val Leu Pro Gly Ala Val
165 170 175
Gly Phe Asn Pro Ala Pro Gly Val Tyr Arg Met Asn Pro Ser Tyr Leu
180 185 190
Pro Leu Phe Leu Met Arg Lys Leu Ser Glu Ala His Ser Asp Ser Phe
195 200 205
Trp Lys Ser Gln Pro Glu Leu Ser Ala Gln Leu Ile Val Asn Ser Ala
210 215 220
Pro Lys Gly Tyr Val Pro Asp Trp Val Thr Val Lys Ala Asp Gly Ile
225 230 235 240
Ser Ala Asp Ile Glu Lys Gly Asn Val Gly Ser Tyr Asp Ala Ile Arg
245 250 255
Thr Tyr Leu Trp Ala Gly Leu Thr Ala Arg Asp Glu Pro Glu Arg Lys
260 265 270
Ala Leu Met Gln Ala Val Tyr Ala Tyr Met Gln Leu Ser Asn Arg Leu
275 280 285
Gly Glu Pro Leu Glu Arg Val Asp Thr Ser Ser Gly Ile Trp Tyr Gly
290 295 300
Thr Ala Ala Pro Gly Phe Tyr Tyr Ala Leu Glu Pro Leu Ala Gln Glu
305 310 315 320
Leu Arg Leu Ser Arg Leu Leu Leu Ala Ile Lys Ala Lys His Gln Glu
325 330 335
Val Lys Asn Gln Asn Tyr Ala Gln Leu Ser Tyr Tyr Asp Trp Val Leu
340 345 350
Phe Leu Tyr Ala Lys Gly Trp Val Glu Gly Arg Tyr Arg Phe Asp Lys
355 360 365
Arg Gly Ala Leu Gln Val Ser Trp Asn Lys Lys Cys
370 375 380
<210> 36
<211> 369
<212> PRT
<213> 肺炎克雷伯氏菌
<400> 36
Met Met Lys Val Leu Cys Gly Ala Val Leu Ser Ala Leu Leu Leu Ala
1 5 10 15
Ala Gly Gln Val Gly Ala Ala Cys Gln Trp Pro Ala Trp Glu Gln Phe
20 25 30
Lys Gln Ala Tyr Val Ser Pro Glu Gly Arg Val Ile Asp Pro Ser Asp
35 40 45
Ala Arg Lys Ile Ser Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Ala Ala Asn Asp Arg Ala Gly Phe Asp Lys Leu Leu Thr Trp
65 70 75 80
Thr Gln Asn Asn Leu Ala Glu Gly Asp Leu Lys Gln His Leu Pro Gly
85 90 95
Trp Leu Trp Gly Lys Lys Asp Asp Glu Gln Trp Thr Leu Leu Asp Ser
100 105 110
Asn Ser Ala Ser Asp Ser Asp Leu Trp Ile Ala Trp Ala Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Gln Gln Pro Gln Tyr Thr Glu Thr Gly Lys Ala
130 135 140
Leu Leu Ala Arg Ile Val Ala Glu Glu Thr Val Ala Val Pro Gly Leu
145 150 155 160
Gly Thr Met Leu Leu Pro Gly Lys Val Gly Phe Ala Asp Asp Arg Gly
165 170 175
Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Gln Leu Ala Thr Tyr Phe
180 185 190
Val Arg Phe Gly Ala Pro Trp Pro Ala Leu Arg Asp Ser Asn Leu Arg
195 200 205
Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Thr Pro Asp Trp Val Arg
210 215 220
Tyr Glu Lys Gly Lys Gly Trp Gln Leu Lys Thr Glu Lys Pro Pro Ile
225 230 235 240
Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Val Gly Met Leu His
245 250 255
Asp Gly Asp Lys Gln Lys Ala Arg Leu Leu Gln Arg Phe Ala Pro Met
260 265 270
Ala Ala Gln Thr Thr Glu Gln Gly Val Pro Pro Glu Lys Val Asn Ile
275 280 285
Ala Thr Gly Lys Thr Ser Gly Gln Gly Pro Val Gly Phe Ser Ala Ala
290 295 300
Met Leu Pro Phe Leu Gln Asp Asp Glu Ala Arg Ser Val Gln Arg Gln
305 310 315 320
Arg Val Ala Asp Asn Tyr Pro Gly Ala Asp Ala Tyr Tyr Ser Ala Val
325 330 335
Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Thr
340 345 350
Ala Ser Gly Glu Leu Gln Pro Asp Trp Asn Gln Glu Cys Ala Ser Ser
355 360 365
His
<210> 37
<211> 333
<212> PRT
<213> 肺炎克雷伯氏菌
<400> 37
Met Pro Leu Arg Ala Leu Val Ala Met Ile Val Thr Thr Val Val Met
1 5 10 15
Leu Val Pro Arg Ala Trp Ala Asp Thr Ala Trp Glu Arg Tyr Lys Ala
20 25 30
Arg Phe Met Met Pro Asp Gly Arg Ile Ile Asp Thr Ala Asn Gly Asn
35 40 45
Val Ser His Thr Glu Gly Gln Gly Phe Ala Met Leu Leu Ala Val Ala
50 55 60
Asn Asn Asp Arg Pro Ala Phe Asp Lys Leu Trp Gln Trp Thr Asp Asn
65 70 75 80
Thr Leu Arg Asn Lys Ser Asn Gly Leu Phe Tyr Trp Arg Tyr Asn Pro
85 90 95
Val Ala Pro Asp Pro Ile Ala Asp Lys Asn Asn Ala Ser Asp Gly Asp
100 105 110
Thr Leu Ile Ala Trp Ala Leu Leu Arg Ala Gln Lys Gln Trp Gln Asp
115 120 125
Lys Arg Tyr Ala Ile Ala Ser Asp Ala Ile Thr Ala Ala Leu Leu Lys
130 135 140
Ser Thr Val Val Ser Phe Ala Gly Arg Gln Val Met Leu Pro Gly Val
145 150 155 160
Lys Gly Phe Asn Leu Asn Asp His Leu Asn Leu Asn Pro Ser Tyr Phe
165 170 175
Ile Phe Pro Ala Trp Arg Ala Phe Ala Glu Arg Thr His Leu Thr Ala
180 185 190
Trp Arg Thr Leu Gln Ser Asp Gly Gln Ala Leu Leu Gly Gln Met Gly
195 200 205
Trp Gly Lys Ser His Leu Pro Ser Asp Trp Val Ala Leu Arg Ala Asp
210 215 220
Gly Lys Met Leu Pro Ala Lys Glu Trp Pro Pro Arg Met Ser Phe Asp
225 230 235 240
Ala Ile Arg Ile Pro Leu Tyr Leu Ser Trp Ala Asp Pro His Ser Ala
245 250 255
Leu Leu Ala Pro Trp Lys Ala Trp Met Gln Ser Tyr Pro Arg Leu Gln
260 265 270
Thr Pro Ala Trp Ile Asn Val Ser Thr Asn Glu Val Ala Pro Trp Tyr
275 280 285
Met Ala Gly Gly Leu Leu Ala Val Arg Asp Leu Thr Leu Gly Glu Pro
290 295 300
Gln Glu Ala Pro Gln Ile Asp Asp Lys Asp Asp Tyr Tyr Ser Ala Ser
305 310 315 320
Leu Lys Leu Leu Val Trp Leu Ala Lys Gln Asp Gln Arg
325 330
<210> 38
<211> 344
<212> PRT
<213> Sinorhizobium medicae
<400> 38
Met Lys Pro Leu Val Val Leu Met Leu Leu Leu Leu Ala Tyr Pro Thr
1 5 10 15
His Ala Gln Glu Pro Ala Thr Val Glu Ala Thr Ala Trp Gln Lys Tyr
20 25 30
Lys Thr Arg Phe Leu Asp Pro Gly Gly Arg Ile Ile Asp Asp Ala Asn
35 40 45
Gly Asp Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Leu Leu Leu Ala
50 55 60
Phe Leu Ala Gly Ser Arg Ala Asp Phe Glu Leu Ile Trp Ser Phe Thr
65 70 75 80
Arg Arg Glu Leu Leu Leu Arg Asp Asp Gly Leu Ala Ala Trp Lys Trp
85 90 95
Ser Pro Gly Glu Ala Pro His Val Ser Asp Thr Asn Asn Ala Thr Asp
100 105 110
Gly Asp Ile Leu Ile Ala Tyr Ala Leu Ala Arg Ala Gly Val Ser Trp
115 120 125
Asp Arg Lys Asp Tyr Thr Arg Ala Ala Thr Ala Leu Ala Glu Ala Ile
130 135 140
Leu Glu Lys Thr Val Val Glu His Gly Gly Leu Thr Leu Leu Leu Pro
145 150 155 160
Gly Ala Gln Gly Phe Ser Ala Ala Asp Arg Ala Asp Gly Pro Val Ile
165 170 175
Asn Pro Ser Tyr Trp Val Phe Glu Ala Phe Pro Val Leu Glu Gln Leu
180 185 190
Val Pro Ser Pro Ala Trp Lys Ala Leu Ala Ala Asp Gly Glu Ala Ile
195 200 205
Leu Lys Arg Leu Glu Phe Gly Pro Lys Lys Leu Pro Ala Asp Trp Ile
210 215 220
Ser Ala Arg Thr Val Phe Lys Pro Ala Glu Gly Phe Pro Ser Glu Tyr
225 230 235 240
Gly Tyr Asn Ala Leu Arg Ile Pro Leu Tyr Leu Val Arg Ser Gly Arg
245 250 255
Thr Asp Ser Glu Leu Leu Ser Arg Ile Tyr Arg Gly Met Ser Asp Ala
260 265 270
Lys Gly Ala Val Leu Leu Ser Asp Val Glu Ser Gly Ala Val Glu Glu
275 280 285
Thr Leu Thr Asp Pro Gly Tyr Arg Ile Ile Asn His Ile Leu Ala Cys
290 295 300
Val Leu Gln Gly Thr Lys Leu Pro Asp Asp Met Lys Thr Phe Glu Pro
305 310 315 320
Thr Gln Tyr Tyr Pro Ser Thr Met His Leu Leu Gly Leu Ser Phe Val
325 330 335
Glu Glu Met Arg Pro Glu Cys Leu
340
<210> 39
<211> 417
<212> PRT
<213> 海单胞菌属
<400> 39
Met Asn Tyr Phe Gln Lys Ala Leu Ile Lys Leu Leu Ala Tyr Ser Leu
1 5 10 15
Trp Ile Phe Ser Ser Ser Ser Ile Gln Ala Thr Pro Pro Ala Ser Val
20 25 30
Ile Lys Pro Ser Gly Tyr Ser Gln Pro Glu Leu Asp Thr Ala Leu Leu
35 40 45
Asn Phe Tyr Gln Asp Trp Lys Glu Thr Tyr Leu Arg Gln Gln Cys Gly
50 55 60
Val Gly Arg Tyr Leu Val Asp Met Ala Ala Asp Gly Asn Thr Val Glu
65 70 75 80
Gly Gly Thr Ala Ala Ser Thr Leu Thr Thr Ser Glu Ala His Gly Tyr
85 90 95
Gly Met Leu Ile Thr Val Met Met Ala Asp Phe Asp Pro Gln Ala Arg
100 105 110
Arg Ile Phe Asp Gly Met Val Asp Phe Phe His Asp His Pro Ala Gln
115 120 125
Ser Ala Pro Gly Leu Met Ala Trp Asn Gln Leu Arg Asn Cys Lys Asn
130 135 140
Ala Gly Glu Asn Val Gly Gly Ser Asn Ser Ala Ser Asp Gly Asp Met
145 150 155 160
Asp Ile Ala Tyr Ala Phe Leu Leu Ala Asp Lys Lys Trp Gly Ser Ser
165 170 175
Gly Lys Ile Asn Tyr Lys His Glu Ala Val Asn Ala Ile Thr Ala Ile
180 185 190
Lys Gln His Glu Val Asp Pro Lys Asn His Phe Ile Arg Leu Gly Asp
195 200 205
Trp Val Asp Asp Ile Asp Asp Gly Gln Tyr Ala Asn Thr Thr Arg Ser
210 215 220
Ser Asp Phe Met Val Ser His Leu Lys Ala Tyr Ala Asp Ala Thr Gly
225 230 235 240
Asp Lys Ser Trp Asn Lys Val Arg Asp Thr Thr Tyr Ala Ile Met Ala
245 250 255
Asn Ile Arg Gln Thr Asp Ser Pro Lys Thr Ala Leu Met Pro Asp Phe
260 265 270
Ile Val Gly Leu Pro Asn His Pro Gln Pro Ala Lys Ala Met Phe Leu
275 280 285
Glu Gly Glu Asp Asp Gly Ala Tyr Ser Trp Asn Ala Ala Arg Tyr Pro
290 295 300
Trp Arg Val Ala Leu Asp Tyr Arg Leu Tyr Gly Asp Lys Arg Ala Leu
305 310 315 320
Ser Ala Leu Ala Pro Ile Asn Gln Trp Ile Ile Asn Ile Thr His Gly
325 330 335
Asp Pro Lys Lys Ile Ala Asp Thr Tyr Ala Leu Asp Gly His Tyr Ser
340 345 350
Lys Asp Ser Gly Phe Asp Ser Met Ala Phe Ala Ser Met Phe Ala Val
355 360 365
Ser Ala Thr Ile Asp Pro Ser Asn Gln Ala Trp Leu Asn Ala Leu Trp
370 375 380
Glu Asn Ile Lys Asp Lys Pro Val Glu Ser Glu Asp Tyr Phe Gly Asn
385 390 395 400
Thr Leu Lys Met Leu Ala Met Ile Thr Ile Thr Gly His Trp Asp Lys
405 410 415
Pro
<210> 40
<211> 379
<212> PRT
<213> 青春双歧杆菌
<400> 40
Met Thr Asn Ala Thr Asp Thr Asn Lys Thr Leu Gly Glu Ser Met Phe
1 5 10 15
Ala Gln Cys Gly Tyr Ala Gln Asp Ala Ile Asp Lys Arg Val Ser Gln
20 25 30
Val Trp Tyr Glu Ile Phe Glu Gly Pro Asn Lys Phe Tyr Trp Glu Asn
35 40 45
Asp Glu Gly Leu Ala Tyr Val Met Asp Thr Gly Asn Asn Asp Val Arg
50 55 60
Thr Glu Gly Met Ser Tyr Ala Met Met Ile Ala Leu Gln Tyr Asp Arg
65 70 75 80
Lys Asp Val Phe Asp Lys Leu Trp Gly Trp Val Met Arg His Met Tyr
85 90 95
Met Lys Asp Gly His His Ala His Tyr Phe Ala Trp Ser Val Ala Pro
100 105 110
Asp Gly Thr Pro Asn Ser Asn Gly Pro Ala Pro Asp Gly Glu Glu Tyr
115 120 125
Phe Ala Met Asp Leu Phe Leu Ala Ser Arg Arg Trp Gly Asp Gly Glu
130 135 140
Asp Ile Tyr Glu Tyr Ser Ala Trp Gly Arg Glu Ile Leu Arg Tyr Cys
145 150 155 160
Val His Lys Gly Glu Arg Tyr Asp Gly Glu Pro Met Trp Asn Pro Asp
165 170 175
Asn Lys Leu Ile Lys Phe Ile Pro Glu Thr Glu Trp Ser Asp Pro Ser
180 185 190
Tyr His Leu Pro His Phe Tyr Glu Val Phe Ala Glu Glu Ala Asp Glu
195 200 205
Glu Asp Arg Pro Phe Trp His Glu Ala Ala Ala Ala Ser Arg Arg Tyr
210 215 220
Leu Gln Ala Ala Cys Asp Glu Arg Thr Gly Met Asn Ala Glu Tyr Ala
225 230 235 240
Asp Tyr Asp Gly Lys Pro His Val Asp Glu Ser Asn His Trp His Phe
245 250 255
Tyr Ser Asp Ala Tyr Arg Thr Ala Ala Asn Ile Gly Leu Asp Ala Ala
260 265 270
Trp Asn Gly Pro Gln Glu Val Leu Cys Asp Arg Val Ala Ala Leu Gln
275 280 285
Arg Phe Phe Leu Thr His Asp Arg Thr Ser Val Tyr Ala Ile Asp Gly
290 295 300
Thr Ala Val Asp Glu Val Val Leu His Pro Val Gly Phe Leu Ala Ala
305 310 315 320
Thr Ala Gln Gly Ser Leu Ala Ala Val His Ser Ala Gln Pro Asp Ala
325 330 335
Glu His Asn Ala Arg Glu Trp Val Arg Met Leu Trp Asn Thr Pro Met
340 345 350
Arg Thr Gly Thr Arg Arg Tyr Tyr Asp Asn Phe Leu Tyr Ala Phe Ala
355 360 365
Met Leu Ala Leu Ser Gly Lys Tyr Arg Tyr Glu
370 375
<210> 41
<211> 371
<212> PRT
<213> 假结核耶尔森氏菌
<400> 41
Met Val Val Met Phe Lys His Leu Ala Ser Met Phe Leu Leu Leu Ala
1 5 10 15
Ser Phe Ser Leu Ala Ala Ala Ser Asn Trp Pro Ala Trp Gln Gln Phe
20 25 30
Lys Gln Asp Tyr Ile Ser Glu Gly Gly Arg Ile Ile Asp Pro Gly Ser
35 40 45
Pro Ser Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Val Ala Asp Asp Gln Pro Met Phe Glu Arg Leu Leu Ala Trp
65 70 75 80
Thr Glu Asn Asn Leu Ala Ala Gly Asp Leu Thr Ser Arg Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Asn Ser Gln Asn Asn Trp Asp Ile Leu Asp Pro
100 105 110
Asn Ser Ala Ser Asp Ala Asp Ile Leu Ile Ala Tyr Asn Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Gly Asn Arg Arg Tyr Leu Ile Met Gly Thr Leu
130 135 140
Leu Leu Gln Arg Ile Ala Gln Glu Glu Val Met Asp Ile Pro Gly Leu
145 150 155 160
Gly Gln Met Leu Leu Pro Gly Lys Ile Gly Phe Asn Asp Glu Asp Thr
165 170 175
Trp Arg Leu Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala Arg Phe
180 185 190
Ser Ser Ile Asp Gly Pro Trp Glu Ala Met Val Glu Val Asn Gln Arg
195 200 205
Met Trp Leu Glu Thr Ala Pro Asn Gly Phe Ser Pro Asp Trp Val Val
210 215 220
Trp Gln Lys Gly Lys Gly Trp Gln Pro Asp Thr Ile Lys Pro Asp Val
225 230 235 240
Gly Ser Asn Asp Ala Ile Leu Val Tyr Leu Trp Ala Gly Met Leu Ala
245 250 255
Met Asp Ser Pro Gln Lys Ala Glu Leu Ile Ala Arg Phe Gln Pro Met
260 265 270
Ala Val Ile Thr Gln Gln Gln Gly Leu Pro Pro Phe Thr Thr Asn Ser
275 280 285
Asp Asn Gly Lys Thr Asn Gly Asp Gly Ser Val Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Phe Leu Ala Ser Ser Pro Glu Pro Phe Asn Gln Gln Thr
305 310 315 320
Leu Asn Leu Gln Gln Arg Arg Val Gln Asn Ser Pro Pro Gly Ala Asp
325 330 335
Asp Tyr Tyr Ser Ala Ile Leu Thr Leu Phe Gly Gln Gly Trp Leu Gln
340 345 350
His Arg Tyr His Phe Thr His Gln Gly Glu Leu Gln Pro Ser Trp His
355 360 365
Arg Gln Arg
370
<210> 42
<211> 345
<212> PRT
<213> Asaia bogorensis
<400> 42
Met Val Arg Arg Gln Phe Cys Arg Asp Ser Ile Leu Ala Ala Ala Ser
1 5 10 15
Phe Gly Ile Ala Gly Glu Ala Phe Ala Gln Thr Gln Gly Leu Gly Asp
20 25 30
Pro Asp Trp Glu Leu Tyr Lys Lys Lys Tyr Leu Cys Pro Glu Gly Arg
35 40 45
Ile Val Asp Thr Gly Asn Gln Asp Val Ser His Ser Glu Gly Gln Gly
50 55 60
Tyr Gly Leu Leu Phe Ala Ser Thr Phe Asn Asp Gln Ala Ala Phe Asp
65 70 75 80
Gln Ile Trp Ala Trp Thr Arg Asp Asn Leu Lys His Lys Asp Gly Ser
85 90 95
Leu His Ser Trp Arg Trp Thr Pro Thr Ala Pro His Val Ser Asp Thr
100 105 110
Asn Asn Ala Thr Asp Gly Asp Leu Met Ile Ala Trp Ala Leu Arg Arg
115 120 125
Ala Ala Ile Leu Trp Arg Ser Glu Ala Tyr Leu Asp Gln Ala Lys Ala
130 135 140
Ile Leu His Asp Leu Ala Glu Lys Cys Val Arg Lys Val Gly Ser Arg
145 150 155 160
Leu Val Leu Leu Pro Gly Met Lys Gly Phe Asp Arg Pro Lys Ser Val
165 170 175
Ile Ile Asn Leu Ser Tyr Tyr Leu Leu Pro Ala Leu Asp Arg Ala Val
180 185 190
Leu Leu Asp Pro Ser Gly Pro Trp Lys Ser Val Ser Glu His Gly Gln
195 200 205
Gln Leu Leu Gln Ser Cys Arg Phe Gly Leu Trp Gly Leu Pro Pro Asp
210 215 220
Trp Leu Ala Ile Asp Arg Lys Ser Glu Arg Pro Val Pro Ala Pro Gly
225 230 235 240
Tyr Pro Pro Arg Phe Ser Tyr Asp Ala Ile Arg Ile Pro Leu Tyr Phe
245 250 255
Arg Trp Gly Lys Arg Leu Pro Ala Thr Leu Thr Gln Ser Leu Tyr Ala
260 265 270
Val Ser Gln Asn Tyr Ser Met Thr Ala Leu Pro Ala Trp Val Asp Leu
275 280 285
Met Thr Gly Glu Arg Ser Pro Tyr Asn Ala Pro Pro Gly Phe Arg Ala
290 295 300
Val Tyr Leu Phe Ala Leu Asn Gly Ser Asp Ala Leu Ser Asp Leu Pro
305 310 315 320
Asn Ile Lys Glu Ser Ser Asp Tyr Tyr Ser Ser Ser Leu Thr Leu Leu
325 330 335
Ala Arg Ile Ala Pro Met Glu Met Val
340 345
<210> 43
<211> 365
<212> PRT
<213> 大肠杆菌
<400> 43
Met Arg Ser Gly Ile Val Thr Met Leu Leu Leu Ala Ala Phe Ser Val
1 5 10 15
Gln Ala Ala Cys Thr Trp Pro Ala Trp Glu Gln Phe Lys Lys Asp Tyr
20 25 30
Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser Asp Ala Arg Lys Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe Phe Ala Leu Ala Ala
50 55 60
Asn Asp Arg Val Ala Phe Asp Asn Ile Leu Asp Trp Thr Gln Asn Asn
65 70 75 80
Leu Ala Gln Gly Ser Leu Lys Glu Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Lys Glu Asn Ser Lys Trp Glu Val Leu Asp Ser Asn Ser Ala Ser
100 105 110
Asp Gly Asp Val Trp Met Ala Trp Ser Leu Leu Glu Ala Gly Arg Leu
115 120 125
Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser Ala Leu Leu Lys Arg
130 135 140
Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly Leu Gly Ser Met Leu
145 150 155 160
Leu Pro Gly Lys Val Gly Phe Ala Glu Asp Asn Ser Trp Arg Phe Asn
165 170 175
Pro Ser Tyr Leu Pro Pro Thr Leu Ala Gln Tyr Phe Thr Arg Phe Gly
180 185 190
Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln Arg Leu Leu Leu Glu
195 200 205
Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg Tyr Glu Lys Asp
210 215 220
Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile Ser Ser Tyr Asp
225 230 235 240
Ala Ile Arg Val Tyr Met Trp Val Gly Met Met Pro Asp Ser Asp Pro
245 250 255
Gln Lys Ala Arg Met Leu Asn Arg Phe Lys Pro Met Ala Thr Phe Thr
260 265 270
Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp Val Ala Thr Gly Lys
275 280 285
Ala Gln Gly Lys Gly Pro Val Gly Phe Ser Ala Ala Met Leu Pro Phe
290 295 300
Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln Arg Val Ala Asp
305 310 315 320
Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val Leu Thr Leu Phe
325 330 335
Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Ser Thr Lys Gly Glu
340 345 350
Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn Ser His
355 360 365
<210> 44
<211> 369
<212> PRT
<213> Citrobacter koseri
<400> 44
Met Met Asn Ala Leu Arg Gly Trp Met Leu Thr Ala Leu Met Leu Ala
1 5 10 15
Thr Val Ser Val Gln Ala Ala Cys Thr Trp Pro Ala Trp Ile Gln Phe
20 25 30
Lys Lys Asp Tyr Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser Asp
35 40 45
Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe
50 55 60
Ala Leu Ala Ala Asn Asp Arg Pro Ala Phe Asp Leu Ile Leu Asp Trp
65 70 75 80
Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Gln Ser His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Lys Asp Pro Ser Thr Trp Ala Val Leu Asp Ala
100 105 110
Asn Ser Ala Ser Asp Gly Asp Ile Trp Met Ala Trp Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Lys Thr Ser Arg Tyr Thr Asp Thr Gly Ala Ala
130 135 140
Leu Leu Lys Arg Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly Leu
145 150 155 160
Gly Ser Met Leu Leu Pro Gly Asn Val Gly Phe Ala Glu Lys Thr Arg
165 170 175
Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Gln Leu Ala Gln Tyr Phe
180 185 190
Thr Arg Phe Gly Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Met Arg
195 200 205
Leu Leu Met Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg
210 215 220
Tyr Glu Lys Asp Lys Gly Trp Gln Leu Lys Pro Glu Lys Thr Leu Val
225 230 235 240
Ser Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met His
245 250 255
Asp Gly Asp Pro Gln Lys Ala Arg Leu Leu Thr Arg Leu Lys Pro Met
260 265 270
Ala Thr Leu Thr Thr Lys Asn Gly Val Pro Pro Glu Lys Val Asp Val
275 280 285
Ala Ser Gly Lys Ala Gln Gly Asn Gly Pro Val Gly Phe Ser Ala Ser
290 295 300
Leu Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln
305 310 315 320
Arg Val Ala Asp Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Ser Tyr Val
325 330 335
Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Thr
340 345 350
Ala Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Ser Ser
355 360 365
Asn
<210> 45
<211> 370
<212> PRT
<213> 变形斑沙雷氏菌
<400> 45
Met Pro Ala Ala Leu Lys Arg Leu Thr Phe Gly Met Leu Leu Leu Cys
1 5 10 15
Ser Phe Ser Ala Val Ala Thr Cys Glu Trp Pro Ala Trp Gln Gln Tyr
20 25 30
Lys Gln Phe Tyr Ile Ser Glu Gln Gly Arg Val Ile Asp Pro Ser Ser
35 40 45
Pro Asn Arg Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Val Ala Asn Asp Arg Pro Ala Phe Asp Gln Leu Leu Thr Trp
65 70 75 80
Thr Glu Asn Asn Leu Ala Ala Gly Asp Leu Ser Ala His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Glu Asp Asp Lys Lys Gln Trp Thr Val Leu Asp Ser
100 105 110
Asn Ser Ala Ser Asp Ser Asp Leu Trp Ile Ala Tyr Asn Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Lys Ser Arg Arg Tyr Gln Thr Leu Gly Thr Leu
130 135 140
Leu Leu Gln Arg Ile Ala Arg Glu Glu Val Ala Asp Ile Pro Gly Leu
145 150 155 160
Gly Leu Met Leu Leu Pro Gly Lys Val Gly Phe Val Ala Glu Asp Arg
165 170 175
Trp Arg Leu Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala Arg Phe
180 185 190
Ala Ala Leu Asn Gly Pro Trp Arg Ala Met Gln Gln Thr Asn Gln Arg
195 200 205
Leu Trp Leu Glu Thr Ala Pro Tyr Gly Phe Leu Pro Asp Trp Val Val
210 215 220
Trp Gln Val Gly Lys Gly Trp Gln Pro Asp Thr Val Lys Pro Asn Ile
225 230 235 240
Gly Ser Tyr Asp Ala Ile Arg Ala Tyr Leu Trp Ala Gly Met Leu Ala
245 250 255
Asp Asp Asp Gln His Lys Ala Ala Leu Leu Lys Gln Leu Gln Pro Met
260 265 270
Ala Gln Leu Thr Val Gln Gln Gly Val Pro Pro Glu Lys Thr Asp Thr
275 280 285
Ala Ser Gly Lys Thr Met Gly Asn Gly Pro Val Gly Phe Ser Ala Ser
290 295 300
Met Leu Pro Met Leu Ala Asn Gln Pro Glu Ala Leu Gln Val Gln Arg
305 310 315 320
Gln Arg Ile Lys Gln Asn Pro Pro Gly Asp Asn Ala Tyr Phe Ser Ala
325 330 335
Ser Leu Ala Leu Phe Gly Gln Gly Trp Asp Gln Gln Arg Tyr Arg Phe
340 345 350
Asn Arg Gln Gly Glu Leu Gln Pro Ser Trp Gly Gly Gln Cys Val Thr
355 360 365
Ser Glu
370
<210> 46
<211> 369
<212> PRT
<213> 坎氏弧菌
<400> 46
Met Lys Lys Leu Leu Thr Leu Leu Ile Leu Ile Ala Ser Pro His Leu
1 5 10 15
Trp Ala Ser Asp Cys Gly Trp Pro Gln Trp Asp Thr Phe Lys Ala Val
20 25 30
Tyr Ile Asp Gln Gly Arg Val Val Asp Gly Ser Asp Asp Arg Ile Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala
50 55 60
Asn Asp Gln Lys Thr Phe Ala Asp Val Leu Asn Trp Thr Gln Val His
65 70 75 80
Leu Ala Asp Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Arg Lys Glu Asn Gly Ser Phe Gly Val Leu Asp Thr Asn Ala Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ala Leu Val Glu Ala Gly Arg Leu
115 120 125
Trp Asp Asn Tyr Tyr Tyr Gln Ser Leu Gly His Leu Leu Ala Ser Arg
130 135 140
Ile Leu Arg Glu Glu Ser Val Ser Ile Asp Gly Thr Gly Thr Leu Leu
145 150 155 160
Leu Pro Ala Pro Thr Gly Phe Glu Phe Ser Gln Arg Tyr Arg Val Asn
165 170 175
Pro Ser Tyr Val Pro Leu Gln Leu Val Thr Arg Met Glu Ala Leu Tyr
180 185 190
Pro Gln Tyr Ser Trp Asp Thr Met Tyr Gln Ala Ser Val Gln Met Leu
195 200 205
Thr His Thr Met Pro Lys Gly Phe Ser Pro Asp Trp Ala Thr Leu Ser
210 215 220
Gln Gly Glu Tyr Ser Val Asp Ser Val Thr Gly Pro Ser Gly Ser Tyr
225 230 235 240
Asn Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Asp Asp Gln Val
245 250 255
Ala Glu Lys Ala Gln Leu Ile Gln Gln Met Gln Pro Phe Val Thr Ala
260 265 270
Thr Lys Thr Leu Ser Ala Pro Pro Arg Asp Val Asn Thr Glu Thr Gly
275 280 285
Gly Tyr Thr Gln Ala Gly Ser Ala Gly Phe Ser Ala Ala Val Leu Pro
290 295 300
Leu Leu Ala Ser Ser Gly Glu Ser Glu Leu Leu Lys Ala Gln Ala Ser
305 310 315 320
Arg Ala Gln Gln Met Leu Ile Ser Asp Arg Asn Asp His Tyr Tyr Asp
325 330 335
Asn Val Leu Ser Leu Phe Gly Leu Gly Trp His Gln Ala Arg Phe Arg
340 345 350
Phe Gly Lys His Gly Glu Leu Leu Pro Ala Trp Ser Glu Gln Cys Gln
355 360 365
Glu
<210> 47
<211> 414
<212> PRT
<213> Burkholderia multivorans
<400> 47
Met Ala Gln Ala Ile Ala Thr Arg Cys Ala Asp Ser Ala Arg Arg Leu
1 5 10 15
Gly Ala Thr Phe Val Leu Ala Val Ala Ala Ala Cys Val Ala Gly Gly
20 25 30
Thr Ala Ala Arg Ala Ala Thr Ala Pro Arg Thr Val Gly Gly Ala Ala
35 40 45
Ser Ala Thr Asp Ala Ala Asp Ala Arg Cys Ala Ala Asp Trp Pro Arg
50 55 60
Trp Asp Ala Phe Lys Arg Ala Phe Val Ser Ala Asp Gly Arg Val Ile
65 70 75 80
Asp Val Gly Ser Ala Asp Ser Arg Thr Val Ser Glu Gly Gln Ala Tyr
85 90 95
Gly Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Arg Thr Phe Asp Thr
100 105 110
Ile Leu Ala Trp Thr Glu Asn Asn Leu Ala Gln Gly Asp Leu Ser Ala
115 120 125
Arg Leu Pro Ala Trp Leu Trp Gly Arg Ala Pro Asp Gly Thr Trp Arg
130 135 140
Val Leu Asp Ala Asn Ala Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr
145 150 155 160
Thr Leu Val Glu Ala Gly Arg Leu Trp Arg Glu Arg Ser Tyr Thr Ala
165 170 175
Arg Gly Thr Leu Leu Ala Lys Arg Val Leu Asp Asp Glu Thr Ala Asn
180 185 190
Val Pro Gly Leu Gly Leu Thr Leu Leu Pro Gly Pro Thr Gly Phe Ala
195 200 205
Leu Ala Arg Asp Arg Trp Arg Val Asn Pro Ser Tyr Ser Pro Pro Gln
210 215 220
Val Ile Arg Ala Leu Ala Val Arg Leu Pro Asp Asp Arg Arg Trp Ala
225 230 235 240
Ala Leu Ala Ser Ser Thr Gly Arg Val Leu Leu Asp Thr Ala Pro Lys
245 250 255
Gly Phe Ala Pro Asp Trp Ala Leu Tyr Arg Ala Gly Glu Gly Phe Gly
260 265 270
Pro Asp Pro Gln Thr His Ala Glu Ser Ala Tyr Asn Ala Ile Arg Val
275 280 285
Tyr Leu Trp Ala Gly Met Leu Asp Arg Ala Asp Pro Leu Ala Ala Pro
290 295 300
Leu Leu Ala Arg Phe Ala Pro Phe Ala Asp Tyr Ile Ala Ala His Gly
305 310 315 320
Ala Pro Pro Glu Lys Val Asp Thr Thr Thr Gly Val Ala Gly Pro Asn
325 330 335
Asp Gly Asn Gly Gly Phe Ser Ala Ala Ala Val Pro Phe Leu Asp Ala
340 345 350
Arg Gly Gln His Ala Leu Ala Asp Ala Gln Ala Ala Arg Val Asp Ala
355 360 365
Leu Ala Arg Glu Ser Ala Pro Gly Tyr Tyr Thr Ser Val Leu Thr Leu
370 375 380
Phe Gly Leu Gly Trp Arg Glu Gly Arg Tyr Arg Phe Gly Ala Asp Gly
385 390 395 400
Ser Leu Asp Val Arg Trp Gly Glu Arg Ser Cys Val Ala Arg
405 410
<210> 48
<211> 543
<212> PRT
<213> 橙色滑柱菌
<400> 48
Met Ala Glu Gln Pro Arg Ser Phe Phe Gly Ser Arg Leu Ile Gln Arg
1 5 10 15
Ala Ile Leu Ile Leu Ala Ile Cys Ala Val Ile Val Pro Leu Phe Ala
20 25 30
Ser Lys Ser Ser Tyr Ala Ala Ala Thr Pro Arg Arg Pro Phe Pro Gln
35 40 45
His Thr Gln Tyr Ala Ser Gly Thr Ile Lys Pro Asn His Arg Ser Gln
50 55 60
Ala Gln Leu Asp Ser Asp Val Lys Ala Phe Tyr Asp Val Trp Lys Ser
65 70 75 80
Arg Tyr Val Val Arg Ala Gly Thr Ser Ser Ala Gly Asn Pro Tyr Tyr
85 90 95
Arg Ile Ser Phe Gly Ser Ser Ala Pro Asn Val Thr Val Ser Glu Gly
100 105 110
Gln Gly Tyr Gly Met Val Ile Met Ala Leu Met Ala Gly Tyr Asp Pro
115 120 125
Glu Ala Gln Thr Ile Phe Asp Gly Leu Trp Glu Phe Ser Arg Thr Asn
130 135 140
Pro Ser Asn Ile Asp Ser Arg Leu Met Gly Trp Arg Ile Pro Ser Asp
145 150 155 160
Gly Ser Gly Asn Asp Ser Ala Phe Asp Gly Asp Ala Asp Ile Ala Tyr
165 170 175
Gly Leu Ile Leu Ala Asp Ala Gln Trp Gly Ser Thr Gly Arg Ile Asn
180 185 190
Tyr Ala Ser Ala Ala Asn Thr Val Leu Asp Gly Val Leu Ser Ser Thr
195 200 205
Ile Gly Pro Asn Ser Arg Leu Pro Met Leu Gly Asp Trp Val Ser Pro
210 215 220
Asn Gly Ser Pro His Ser Gln Tyr Thr Pro Arg Pro Ser Asp Phe Met
225 230 235 240
Pro Ser His Phe Arg Asp Tyr Arg Ala Phe Thr Gly Asn Ala Thr Trp
245 250 255
Asp Thr Val Leu Ser Lys Thr Gln Gly Val Val Asp Ser Ile Gln Ala
260 265 270
Gln Tyr Ser Pro Asn Thr Gly Leu Met Pro Asp Phe Val Val Gln Ala
275 280 285
Asn Thr Thr Pro Lys Pro Ser Pro Ala Asn Phe Leu Glu Ser Glu Asn
290 295 300
Asp Gly Asn Tyr Tyr Tyr Asn Ser Gly Arg Val Pro Trp Arg Leu Gly
305 310 315 320
Ala Asp Ala Val Ile Phe Gly Asp Ala Ala Ser Leu Arg Gln Ala Gln
325 330 335
Lys Ile Ser Arg Trp Ile Glu Gln Ala Thr Gly Gly Thr Ala Ser Asn
340 345 350
Ile Arg Ala Gly Tyr Ser Leu Asn Gly Thr Ala Leu Pro Asp Ser Gly
355 360 365
Tyr Phe Ser Thr Phe Phe Ala Ala Pro Phe Gly Val Ala Ala Met Thr
370 375 380
Val Pro Ala Ser Gln Gln Trp Leu Asn Arg Val Tyr Asp Ala Val Arg
385 390 395 400
Ser Asn His Gln Asp Tyr Phe Glu Asp Thr Val Thr Leu Gln Cys Leu
405 410 415
Leu Leu Met Ser Gly Asn Tyr Trp Ser Pro Ser Arg Ser Ser Thr Ser
420 425 430
Pro Thr Ala Thr Pro Arg Pro Ala Thr Ala Thr Pro Arg Pro Ala Thr
435 440 445
Ala Thr Pro Arg Pro Ala Thr Ala Thr Pro Arg Pro Ala Thr Ala Thr
450 455 460
Pro Arg Pro Ala Thr Ala Thr Pro Arg Pro Ala Thr Ala Thr Pro Arg
465 470 475 480
Pro Ala Thr Ala Thr Pro Gln Pro Ala Thr Ala Thr Pro Asn Gly Val
485 490 495
Ala Ala Trp Asp Gly Asn Met Arg Ala Tyr Lys Val Gly Asp Arg Val
500 505 510
Ser Tyr Asn Gly Arg Ile Tyr Arg Cys Leu Gln Ala His Thr Ser Leu
515 520 525
Ser Thr Trp Thr Pro Glu Ala Val Pro Ala Leu Trp Gln Ala Glu
530 535 540
<210> 49
<211> 511
<212> PRT
<213> 橙色滑柱菌
<400> 49
Met Lys Gln Ala Leu His Ser Arg Trp Leu Ile Leu Val Met Leu Val
1 5 10 15
Gly Leu Gly Leu Ser Leu Pro Arg Gln Gln Ser Ala Ser Ala Asn Pro
20 25 30
Gln Ala Phe Ser Phe Pro Tyr Asn Gln Ala Ser Gly Ile Asn Tyr Asn
35 40 45
Val Thr Asp Leu Thr Gln Ala Trp Asn Glu Trp Lys Ser Ala Gln Ile
50 55 60
Thr Ser Asn Asn Ala Gly Gly Gly Gly Arg Leu Arg Val Leu Gly Gly
65 70 75 80
Val Ser Asn Ser Thr Thr Val Ser Glu Gly Gln Gly Tyr Gly Leu Leu
85 90 95
Phe Ala Ser Leu Phe Asp Asp Gln Ala Thr Phe Asp Gly Leu Trp Leu
100 105 110
Phe Thr Arg Asp Tyr Phe Thr Ser Arg Gly Leu Met His Trp His Ile
115 120 125
Gly Asn Pro Gly Gln Ile Asn Gly Ser Gly Ala Ala Thr Asp Gly Asp
130 135 140
Glu Asp Ile Ala Met Gly Leu Val Asn Ala Cys Ile Lys Val Gln Gln
145 150 155 160
Gly Val Trp Pro Ala Ser Ser Asn Gly Leu Asn Tyr Cys Thr Leu Ala
165 170 175
Ser Thr Met Ile Asn Asn Ile Tyr Thr Tyr Glu Val Asp His Ala Gly
180 185 190
Ser Asn Pro Pro Ala Gly Leu Pro Asn Asn Gln Gly Asn Glu Leu Leu
195 200 205
Pro Gly Asp Ser Trp Ser Thr Ala Thr Glu Tyr Thr Gln Gly Ile Val
210 215 220
Asn Leu Ser Tyr Phe Ser Pro Gly Tyr Ser Thr Val Phe Gly Lys Phe
225 230 235 240
Thr Asn Lys Asn Thr Glu Trp Ala Ala Val Asn Thr Arg Asn Tyr Ala
245 250 255
Ile Thr Asn Leu Val Gln Ala Lys Ala Gly Asn Cys Ser Lys Leu Val
260 265 270
Pro Asn Trp Asn Gln Tyr Asp Gly Asp Val Gln Tyr Val Ser Trp Gln
275 280 285
Pro Glu Glu Ser Ala Trp Trp Ser Tyr Asp Ala Ala Arg Phe Ala Trp
290 295 300
Arg Ile Ala Ile Asp Lys Ala Trp Tyr Asn Thr Ser Asn Ser Arg Glu
305 310 315 320
Thr Met Asn Glu Ile Gly Gly Phe Phe Ser Ser Val Gly Ile Asp Asn
325 330 335
Ile Gln Ala Arg Tyr Arg Leu Asp Gly Thr Ser Val Asp Asn Tyr Arg
340 345 350
Gly Val Phe Phe Val Ala Asn Ala Ala Ala Ala Ile Trp Ala Ala Pro
355 360 365
Ala Pro Gln Ala Val Asn Cys Gly Ala Ala Thr Ala Ser Leu Lys Thr
370 375 380
Thr Pro Gln Gln Ala Tyr Asn Ala Val Leu Ala Thr Lys Asp Thr Pro
385 390 395 400
Asn Ser Tyr Tyr Pro Asn Ala Trp Arg Leu Leu Ser Met Leu Leu Leu
405 410 415
Thr Gly Asn Phe Pro Asn Leu Tyr Glu Met Ala Gln Ser Gly Thr Gly
420 425 430
Gly Thr Ala Thr Pro Thr Ile Thr Pro Thr Ile Thr Arg Thr Pro Thr
435 440 445
Ala Thr Ala Thr Arg Thr Ala Thr Ala Thr Pro Ser Asn Thr Pro Thr
450 455 460
Arg Thr Ser Thr Gly Thr Pro Ala Thr Ile Thr Ile Thr Pro Ser Arg
465 470 475 480
Thr Pro Thr Ala Thr Ile Thr Pro Ser Arg Thr Pro Thr Val Val Pro
485 490 495
Asn Leu Asn Phe Arg Met Tyr Leu Pro Phe Ala Lys Ser Asn Ser
500 505 510
<210> 50
<211> 609
<212> PRT
<213> 橙色滑柱菌
<400> 50
Met Leu Lys His Arg Lys Met Leu Leu Met Val Ala Leu Leu Thr Leu
1 5 10 15
Leu Thr Val Pro Trp Trp Gln Ser Lys Ala Ala Gln Gly Asn Ser Val
20 25 30
Ser Pro Leu Leu Phe Pro Tyr Asn Gln Ala Ser Gly Ile Asn Tyr Asn
35 40 45
Val Thr Asp Leu Thr Gln Ala Trp Asn Glu Trp Lys Ser Asn Met Ile
50 55 60
Thr Ala Asn Asn Ala Gly Gly Gly Asn Arg Leu Arg Val Met Gly Gly
65 70 75 80
Val Asp Ser Ser Ser Thr Val Ser Glu Gly Gln Gly Tyr Gly Ile Leu
85 90 95
Phe Ala Ser Leu Phe Asp Asp Gln Thr Thr Leu Asp Gly Leu Trp Leu
100 105 110
Phe Thr Arg Asp His Leu Asp Pro Asn Gly Leu Met His Trp His Ile
115 120 125
Gly Asn Pro Gly Gln Leu Arg Gly Ser Tyr Ala Ala Thr Asp Gly Asp
130 135 140
Glu Asp Ile Ala Leu Gly Leu Val Asn Ala Cys Val Lys Val Arg Lys
145 150 155 160
Gly Val Trp Pro Asn Ser Ser Asn Gly Leu Asp Tyr Cys Ser Leu Ala
165 170 175
Thr Thr Met Ile Asn Asn Ile Tyr Thr Tyr Glu Val Asp His Pro Gly
180 185 190
Ser Ser Pro Val Ala Gly Leu Pro Ser Asn Pro Gly Asn Glu Leu Leu
195 200 205
Pro Gly Asp Gly Trp Asn Leu Ala Arg Asp Tyr Pro Glu Gly Ile Val
210 215 220
Asn Leu Ser Tyr Phe Ser Pro Gly Tyr Phe Thr Val Phe Gly Lys Phe
225 230 235 240
Thr Gly Lys Thr Ser Glu Trp Glu Ala Val Asn Thr Arg Asn Tyr Glu
245 250 255
Ile Thr Asn Leu Ala Gln Ser Arg Pro Gly Asn Cys Ser Lys Leu Val
260 265 270
Pro Asn Trp Asn Gln Tyr Asp Gly Asp Ala Gln Leu Val Ser Trp Gln
275 280 285
Pro Glu Glu Tyr Ala Trp Trp Ser Tyr Asp Ala Ala Arg Phe Ala Trp
290 295 300
Arg Val Ala Val Asp Lys Ala Trp Tyr Asn Thr Ala Ser Ser Arg Glu
305 310 315 320
Thr Met Asn Glu Val Gly Gly Phe Phe Ser Ser Val Gly Ile Glu Asn
325 330 335
Val Gln Ala Arg Tyr Arg Met Asn Gly Thr Ser Val Asp Asn Tyr Arg
340 345 350
Gly Val Phe Phe Val Ala Asn Ala Ala Ala Ala Ile Trp Ala Ala Pro
355 360 365
Ala Pro Gln Ala Ile Asn Cys Gly Ala Ala Thr Gly Thr Leu Lys Thr
370 375 380
Ser Pro Gln Gln Ala Tyr Asn Met Val Leu Thr Thr Lys Asp Ser Pro
385 390 395 400
Asn Ser Tyr Tyr Val Asn Ala Trp Arg Leu Met Ser Met Leu Leu Leu
405 410 415
Thr Gly Asn Phe Pro Asn Ile Tyr Glu Leu Ala Asn Gly Val Thr Pro
420 425 430
Thr Asn Thr Pro Val Pro Thr Ser Val Pro Pro Thr Asn Thr Ala Val
435 440 445
Pro Thr Asn Val Pro Pro Thr Asn Thr Thr Val Pro Pro Thr Ser Thr
450 455 460
Pro Arg Pro Thr Asn Thr Pro Val Thr Ile Thr Pro Ile Leu Thr Pro
465 470 475 480
Ile Pro Thr Leu Thr Pro Ile Pro Thr Thr Val Pro Thr Ser Val Pro
485 490 495
Pro Thr Asn Thr Pro Ile Ala Gly Ala Cys Gln Ile Thr Tyr Ser Ile
500 505 510
Ser Asn Asp Trp Gly Ser Gly Phe Thr Ala Asp Val Ser Ile Arg Asn
515 520 525
Asn Gly Thr Ala Ile Asn Asn Trp Asn Val Arg Trp Asn Phe Ala Gly
530 535 540
Asn Gln Gln Ile Asn Asn Leu Trp Asn Gly Thr Val Ser Gln Thr Gly
545 550 555 560
Gln Ala Val Ser Val Asn Asn Val Gly Trp Asn Gly Tyr Ile Gly Ser
565 570 575
Gly Gly Thr Ala Ser Phe Gly Phe Gln Ala Ser Tyr Asn Gly Ser Asn
580 585 590
Pro Lys Pro Thr Ser Phe Ser Leu Asn Gly Thr Ala Cys Ser Val Ala
595 600 605
Pro
<210> 51
<211> 351
<212> PRT
<213> 根癌土壤杆菌
<400> 51
Met Lys Ser Gly Ile Ser Lys Ile Ala Gly Ile Val Cys Leu Ser Ala
1 5 10 15
Met Met Leu Phe Ala Gly Leu Ser Leu Ser Arg Ala Ala Ser Ile Pro
20 25 30
Thr Asp Ala Trp Ala Ala Tyr Lys Gly Ala Phe Leu Asp Pro Gly Gly
35 40 45
Arg Ile Ile Asp Thr Gly Asn Ser Asn Ile Ser His Ser Glu Gly Gln
50 55 60
Gly Tyr Gly Met Trp Leu Ala Val Leu Ala Asp Asn Leu Ser Asp Phe
65 70 75 80
Glu Leu Ile Trp Ser Phe Thr Arg Thr Glu Leu Leu Val Arg Asp Asp
85 90 95
Gly Leu Ser Ala Trp Lys Trp Asp Pro Arg Thr Arg Pro His Val Thr
100 105 110
Asp Ile Asn Asn Ala Thr Asp Gly Asp Ile Leu Ile Ala Tyr Ala Leu
115 120 125
Ala Leu Ala Ala Gly Gln Trp Asn Arg Gln Asp Tyr Ala Glu Ala Ser
130 135 140
Ala Ala Ile Ala Ser Val Ile Leu Lys Lys Thr Val Val Gln Arg Gly
145 150 155 160
Gly Arg Thr Leu Leu Leu Pro Ala Ala Ser Gly Phe Gly Glu Gly Asp
165 170 175
Arg Ala Asp Gly Pro Val Val Asn Pro Ser Tyr Leu Ile Phe Glu Ala
180 185 190
Phe Pro Val Leu Asn Leu Val Ala Pro Ser Pro Leu Trp Lys Ala Val
195 200 205
Ala Asp Asp Gly Val Ala Gln Ile Gly Ala Phe Ala Phe Ser Asp Arg
210 215 220
Lys Leu Pro Ala Asp Trp Val Ser Val Lys Thr Lys Pro Gln Pro Ala
225 230 235 240
Ser Gly Phe Gln Pro Glu Phe Gly Tyr Asn Ala Val Arg Ile Pro Leu
245 250 255
Tyr Leu Ala Arg Ala Asn Met Gly Ala Pro Glu Leu Leu Ser Arg Leu
260 265 270
Lys Asp Gly Met Thr Leu Glu Ser Gly Ala Ala Gly Thr Phe Asp Leu
275 280 285
Lys Ser Gly Ala Val Lys Asp Val Leu Ser Asp Ala Gly Tyr Arg Ile
290 295 300
Ile Pro Ala Leu Ala Ala Cys Ile Ala Gly Gly Pro Ala Val Ser Ala
305 310 315 320
Glu Leu Lys Asn Phe Gln Pro Thr Leu Tyr Tyr Pro Ser Thr Leu His
325 330 335
Leu Leu Ala Leu Ser Phe Leu Ala Arg Asn Asn Gly Glu Cys Arg
340 345 350
<210> 52
<211> 383
<212> PRT
<213> 纤维堆囊菌
<400> 52
Met Ser Ser Asp Glu Leu Gly Pro Leu Thr Gly Pro Ser Glu Tyr Pro
1 5 10 15
Asn Ala Phe Arg Asp Val Leu Gly Lys Thr Asp Glu Glu Ile Ala Ala
20 25 30
Lys Ile Glu Asp Ser Phe Glu Gln Leu Phe Tyr Gly Asp Arg Tyr Glu
35 40 45
Glu Thr Val Tyr Tyr Pro Val Pro Gly Thr Glu Gln Ala Tyr Ile Trp
50 55 60
Asp Asp Tyr His Asn Ser Val Arg Ser Glu Gly Ile Gly Leu Gly Met
65 70 75 80
Ile Ile Thr Val Glu Leu Asp Lys Arg Asp Glu Phe Asp Arg Ile Trp
85 90 95
Arg Tyr Ala Lys Ser Thr Leu Gln Leu Lys Ser Gly Pro Gly Gln Gly
100 105 110
Tyr Leu Met Ser Trp Cys Asp Val Pro Gly Gly Thr Ala Val Val Cys
115 120 125
Phe Asp Pro Phe Gly Leu Gln Gln Val Ala Met Ala Leu Phe Phe Ala
130 135 140
His Gly Arg Trp Gly Ser Asp Thr Gly Thr Ile Asp Tyr Gly Met Asp
145 150 155 160
Ala Ile Ser Leu Leu Glu Val Met Arg His Lys Glu Ala Leu Asn Gly
165 170 175
Gly Val Ala Ser Gly Val Thr Asn Thr Phe Asp Pro Thr Thr Lys Leu
180 185 190
Val Tyr Asp Tyr Pro His Val Ser Ala Ala Asp Val Thr Arg Pro Ser
195 200 205
Ile Glu Met Pro Ala Tyr Tyr Glu Leu Trp Ala Gln Ala Met Arg Asp
210 215 220
Pro Phe Trp Ser Glu Ala Ala Ala Ser Ala Arg Thr His Trp Gln Glu
225 230 235 240
Ala Ala His Pro Ser Thr Gly Leu Ile Pro Val Arg Thr His Phe Asp
245 250 255
Gly Thr Pro Val Leu Asn Trp Asp Tyr Phe Gly Ser Glu Ala Tyr Arg
260 265 270
Thr Gln Leu Ser Ile Ala Leu Asp His Val Trp Phe Gly Val Asp Pro
275 280 285
Trp Gln Val Glu Glu Ala Asp Arg Leu Leu Gly Phe Phe Ser Lys Leu
290 295 300
Gly Ile Asp Lys Tyr Gly Ala Val Phe Lys Ile Asn Gly Ala Met Leu
305 310 315 320
Ser Ala Thr Pro Glu Ser Ala Leu Ile Phe Met Asn Gly Val Thr Gly
325 330 335
Leu Ile Ala Thr Asn Asp Asp Arg Ala Ala Tyr Ile Glu Ala Val Trp
340 345 350
Thr Thr Pro Pro Ala Thr Gly Glu Thr Arg Tyr Phe Ser Arg Leu Leu
355 360 365
His Met Leu Ser Leu Leu Thr Leu Ser Gly Gln Phe Arg Val Tyr
370 375 380
<210> 53
<211> 467
<212> PRT
<213> 纤维堆囊菌
<400> 53
Met Asn Arg Asn Ser Leu Arg Phe Leu Ser Val Gly Leu Leu Leu Ala
1 5 10 15
Ala Val Ala Cys Gly Asp Asp Asp Gly Gly Ser Gly Gly Ala Gly Ser
20 25 30
Val Ser Ser Gly Thr Gly Gly Ala Gly Gly Thr Gly Gly Ser Ala Asp
35 40 45
Gly Gly Gly Gly Gly Gly Asp Gly Gly Gly Gly Ala Ser Thr Pro Gly
50 55 60
Gly Ser Gly Ala Val Asp Thr Gly Val Tyr Arg Asn Leu Phe Val Glu
65 70 75 80
Asn Gly Arg Thr Gln Asp Glu Val Asn Ala Lys Ile Thr Ala Ala Trp
85 90 95
Asp His Leu Phe Lys Gly Asp Pro Ala Gln Glu Ala Val Tyr Phe Glu
100 105 110
Gln Glu Glu Asn Asp Asn Gly Pro Leu Ala Gln Ile Arg Asp Ile Ala
115 120 125
Ser Glu Asp Val Arg Ser Glu Gly Met Ser Tyr Gly Met Met Ile Ala
130 135 140
Val Gln Thr Asp His Gln Ala Glu Phe Asp Ala Leu Trp Asn Trp Ala
145 150 155 160
Lys Thr Tyr Met Phe His Ala Asp Glu Thr His Pro Ala Tyr Gly Tyr
165 170 175
Phe Ser Trp Gln Met Asn Phe Asp Gly Thr Pro Leu Asp Glu Met Pro
180 185 190
Ala Pro Asp Gly Glu Glu Tyr Phe Ala Thr Ala Leu Tyr Phe Ala Ser
195 200 205
Gly Arg Trp Gly Asn Gly Glu Gly Ile Tyr Asn Tyr Arg Ala Glu Ala
210 215 220
Asp Arg Leu Leu Asp Leu Met Lys Asn Arg Pro Glu Ile Thr Gly Glu
225 230 235 240
Ser Phe Gly Asn Gly Glu Thr Arg Thr Thr Thr Gly Ser Thr Leu Phe
245 250 255
Asn Thr Glu Asn His Gln Ile Arg Phe Thr Pro Asp Thr Gly Asn Phe
260 265 270
Glu Thr Asn Gly Asp His Thr Asp Pro Ser Tyr His Leu Pro Ala Phe
275 280 285
Tyr Glu Ile Trp Ala Lys Tyr Gly Pro Glu Ala Asp Arg Ala Phe Trp
290 295 300
Ser Glu Ala Ala Glu Val Ser Arg Gly Tyr Phe His Leu Ala Ala His
305 310 315 320
Pro Glu Thr Gly Leu Thr Pro Asp Tyr Ala Asn Phe Asp Gly Thr Pro
325 330 335
Lys Ala Ala Ser Trp Lys Pro Glu Ser Val Asp Phe Arg Phe Asp Ala
340 345 350
Trp Arg Thr Ala Met Asn Trp Ser Val Asp Trp Ala Trp Trp Ala Lys
355 360 365
Asp Glu Arg Gln Gln Glu Leu Ser Asp Arg Ile Gln Ala Phe Phe Glu
370 375 380
Ala Gln Gly Ala Glu Tyr Gly Asn Gln Phe Thr Leu Asp Gly Glu Ala
385 390 395 400
Leu Ser Ala Asp His Ser Ser Gly Leu Val Ala Met Asn Ala Ala Ala
405 410 415
Ser Leu Ala Ala Lys Asp Pro Arg Ser Ala Asp Phe Val Gln Ala Leu
420 425 430
Trp Asp Leu Asp Pro Pro Ser Gly Gln Tyr Arg Tyr Tyr Asp Gly Met
435 440 445
Leu Tyr Phe Met Ala Leu Leu His Val Gly Gly Gln Phe Gln Ala Tyr
450 455 460
Ala Pro Gln
465
<210> 54
<211> 497
<212> PRT
<213> 纤维堆囊菌
<400> 54
Met Leu Thr Ser Pro Val Ala Ser Ala Trp Phe Ala Val Thr Val Ile
1 5 10 15
Ala Cys Ala Pro Ser Asn Gln Gly Thr Gln Val Gly Ser Pro Asp Pro
20 25 30
Ala Ser Ser Ala Asn Pro Ala Gly Ser Ala Ala Pro Ala Ser Thr Ala
35 40 45
Ser Pro Ser Ala Ala Ala Gln Thr Pro Ala Pro Ala Glu Pro Ala Pro
50 55 60
Leu Gly Pro Pro Leu Lys Pro Ala Ala Thr Gly Ala Phe Val Thr Gly
65 70 75 80
Glu Tyr Arg Asn Leu Phe Ala Glu Leu Gly His Lys Pro Ala Glu Ile
85 90 95
Asp Ala Lys Ile Ala Lys Ala Tyr Glu Gln Leu Phe His Gly Asp Pro
100 105 110
Lys Glu Gln Ala Val Leu Phe Pro Ala Gly Lys Asn Ala Asn Gly Pro
115 120 125
Lys Ala Tyr Ile Met Asp Ile Gly Asn Asn Asp Val Arg Ser Glu Gly
130 135 140
Met Ser Tyr Gly Met Met Ile Ala Val Gln Val Asp Arg Lys Asp Asp
145 150 155 160
Phe Asp Ala Leu Trp Asn Trp Ala Lys Ser His Met Tyr Arg Ala Asp
165 170 175
Pro Lys His Pro Gly Phe Gly Tyr Phe Ser Trp Gln Met Arg Pro Asp
180 185 190
Gly Thr Ala Met Asp Glu Asn Pro Ala Pro Asp Ala Glu Glu Tyr Phe
195 200 205
Ala Thr Ala Leu Leu Phe Ala Ser Asn Arg Trp Gly Asn Gly Lys Gly
210 215 220
Ile Tyr Asp Tyr Lys Lys Glu Ala Phe Ala Ile Leu Asp Ala Met Lys
225 230 235 240
Asn Arg Lys Pro Ile Thr Gly Pro Val Asn Lys Asp Lys Arg Lys Thr
245 250 255
Thr Leu His Ser Leu Phe Asn Thr Glu His Lys Met Val Arg Phe Thr
260 265 270
Pro Asp Ala Asp Asn Phe Ala Lys Asn Gly Asp His Thr Asp Pro Ser
275 280 285
Tyr His Leu Pro Ala Phe Tyr Asp Leu Trp Ala Ala Trp Gly Pro Glu
290 295 300
Ala Asp Arg Ala Phe Trp Ala Glu Ala Ala Lys Val Ser Arg Asp Tyr
305 310 315 320
Phe Val Lys Val Thr His Pro Lys Thr Gly Leu Ala Pro Asp Tyr Ala
325 330 335
Asn Phe Asp Gly Thr Pro Lys Ala Ala Ser Trp Asp Ala Gly Thr Ala
340 345 350
Asn Phe Arg Gln Asp Ala Phe Arg Thr Ala Met Asn Trp Ser Val Asp
355 360 365
Ala Ala Trp Trp Ala Lys Asp Pro Arg Gln Thr Glu Leu Ser Asp Arg
370 375 380
Leu Leu Ala Phe Phe Glu Ala Gln Gly Pro Lys Tyr Lys Gly Asn Phe
385 390 395 400
Thr Leu Glu Gly Lys Pro Ile Val Asp Tyr Asp Ser Leu Gly Leu Val
405 410 415
Ala Ala Asn Ala Val Ala Ala Leu Ala Ala Thr His Pro Arg Ala Trp
420 425 430
Arg Phe Val Glu Glu Leu Tyr Gln Arg Glu Ala Pro Thr Gly Lys Trp
435 440 445
Arg Tyr Tyr Asp Gly Met Leu Tyr Thr Met Ser Leu Leu His Leu Ser
450 455 460
Gly Lys Phe Arg Ile Ile Thr Pro Gln Ala Gln Ala Ala Ala Pro Ala
465 470 475 480
Ala Ala Ala Lys Ala Pro Ala Ala Ala Pro Ala Ala Ala Ala Ala Lys
485 490 495
Lys
<210> 55
<211> 381
<212> PRT
<213> Clostridium phytofermentans
<400> 55
Met Asn Gly Ala Ile Lys Ser Gly Tyr Tyr Arg Asn Val Phe Thr Glu
1 5 10 15
Leu Gly Tyr Lys Glu Glu Asp Val Thr Lys Lys Val Glu Asp Ser Phe
20 25 30
Gln Thr Leu Phe Tyr Gly Leu Pro Glu Glu Arg Ile Tyr Tyr Pro Val
35 40 45
Gly Glu Asp Leu Gly Tyr Ile Val Asp Thr Gly Asn His Asp Val Arg
50 55 60
Thr Glu Gly Met Ser Tyr Gly Met Met Met Cys Leu Gln Leu Asp Lys
65 70 75 80
Lys Glu Glu Phe Asp Arg Leu Trp Lys Trp Ala Lys Thr Tyr Met Phe
85 90 95
Met Asp Ser Gly Val Asn Lys Gly Tyr Phe Ala Trp Ser Cys Lys Thr
100 105 110
Asp Gly Thr Lys Asn Ser Tyr Gly Pro Ala Pro Asp Gly Glu Glu Tyr
115 120 125
Phe Ala Leu Ala Leu Phe Phe Ala Ser Asn Arg Trp Gly Asp Gly Asn
130 135 140
Gly Ile Phe Glu Tyr Ser Lys Gln Ala Arg Glu Leu Leu His Glu Cys
145 150 155 160
Ile His Lys Gly Glu Glu Asp Gly Ile Gly Glu Pro Met Trp Glu Pro
165 170 175
Ser Asn Tyr Leu Ile Lys Phe Ile Pro Asn Cys Asn Phe Thr Asp Pro
180 185 190
Ser Tyr His Leu Pro His Phe Tyr Gly Leu Phe Ala Leu Trp Ala Tyr
195 200 205
Glu Glu Asp Arg Glu Phe Phe Lys Lys Ala Ala Glu Ala Ser Arg Ser
210 215 220
Tyr Leu Lys Leu Ala Cys His Glu Lys Thr Gly Leu Cys Ala Glu Tyr
225 230 235 240
Thr Glu Tyr Asp Gly Thr Ala His Ser Gly Asp Gln Glu Ile Phe Gly
245 250 255
Arg His Asp Trp Tyr Tyr Ser Asp Ala Tyr Arg Thr Ile Ala Asn Ile
260 265 270
Gly Leu Asp Tyr Leu Trp Phe Ala Ala Asp Glu Trp Gln Val Thr Cys
275 280 285
Ala Asn His Leu Gln Gln Phe Phe Cys Glu Thr Val Lys Glu His Ala
290 295 300
Ser Gly Ile Tyr Gln Val Asp Gly Thr Ile Ile Lys Gly Glu Ala Leu
305 310 315 320
His Pro Val Ala Ile Ile Ala Thr Asn Ala Gln Ala Ser Leu Ala Ala
325 330 335
Asn Gly Pro Phe Ala Lys Glu Cys Val Asp Lys Phe Tyr His Thr Glu
340 345 350
Leu Arg Thr Gly Asp Arg Arg Tyr Tyr Asp Asn Cys Leu Tyr Met Phe
355 360 365
Ala Leu Leu Ala Leu Ser Gly Lys Tyr Arg Met Trp Met
370 375 380
<210> 56
<211> 369
<212> PRT
<213> Salmonella arizonae
<400> 56
Met Met Thr Met Leu Arg Gly Trp Ile Thr Met Ile Val Met Leu Thr
1 5 10 15
Ala Ile Asn Ala Gln Ala Ala Cys Ser Trp Pro Ala Trp Glu Gln Phe
20 25 30
Lys Lys Asp Tyr Ile Ser Gln Gln Gly Arg Val Ile Asp Pro Gly Asp
35 40 45
Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Met Phe Phe
50 55 60
Ala Leu Ala Ala Asn Asp Arg Pro Ala Phe Ala Gln Leu Phe Asn Trp
65 70 75 80
Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Arg Glu His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Lys Asp Pro Asp Thr Trp Ser Val Leu Asp Ser
100 105 110
Asn Ser Ala Ser Asp Gly Asp Ile Trp Met Ala Trp Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Lys Glu Thr Arg Tyr Thr Glu Val Gly Thr Ala
130 135 140
Leu Leu Lys Arg Ile Ala Arg Glu Glu Val Val Asn Val Pro Gly Leu
145 150 155 160
Gly Ser Met Leu Leu Pro Gly Lys Ile Gly Phe Ala Glu Ala Asn Ser
165 170 175
Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Gln Leu Ala Gln Tyr Phe
180 185 190
Ser Arg Phe Gly Ala Pro Trp Ser Thr Leu Arg Glu Thr Asn Leu Arg
195 200 205
Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg
210 215 220
Tyr Glu Arg Lys Gln Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile
225 230 235 240
Ser Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met His
245 250 255
Asp Gly Asp Pro Gln Lys Ala Arg Leu Leu Ala Lys Phe Lys Pro Met
260 265 270
Ala Thr Leu Thr Met Lys Asn Gly Val Pro Pro Glu Lys Val Asp Val
275 280 285
Ala Ser Gly Asn Ala Gln Gly Thr Gly Pro Val Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln
305 310 315 320
Arg Val Ala Asp His Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val
325 330 335
Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Thr
340 345 350
Val Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Val Ser Ser
355 360 365
Arg
<210> 57
<211> 368
<212> PRT
<213> 副伤寒沙门氏菌
<400> 57
Met Thr Met Leu Arg Gly Trp Ile Thr Met Leu Val Met Leu Thr Ala
1 5 10 15
Ile Asn Ala Gln Ala Ala Cys Ser Trp Pro Ala Trp Glu Gln Phe Lys
20 25 30
Lys Asp Tyr Ile Ser Gln Gln Gly Arg Val Ile Asp Pro Gly Asp Ala
35 40 45
Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Met Phe Phe Ala
50 55 60
Leu Ala Ala Asn Asp Arg Pro Ala Phe Ala Gln Leu Phe Asn Trp Thr
65 70 75 80
Gln Asn Asn Leu Ala Gln Gly Ser Leu Arg Glu His Leu Pro Ala Trp
85 90 95
Leu Trp Gly Gln Lys Asp Pro Asp Thr Trp Ser Val Leu Asp Ser Asn
100 105 110
Ser Ala Ser Asp Gly Asp Ile Trp Met Ala Trp Ser Leu Leu Glu Ala
115 120 125
Gly Arg Leu Trp Lys Glu Thr Arg Tyr Thr Glu Val Gly Thr Ala Leu
130 135 140
Leu Lys Arg Ile Ala Arg Glu Glu Val Leu Asn Val Pro Gly Leu Gly
145 150 155 160
Ser Met Leu Leu Pro Gly Lys Ile Gly Phe Ala Glu Ala Asn Ser Trp
165 170 175
Arg Phe Asn Pro Ser Tyr Leu Pro Pro Gln Leu Ala Gln Tyr Phe Ser
180 185 190
Arg Phe Gly Ala Pro Trp Ser Thr Leu Arg Glu Thr Asn Leu Arg Leu
195 200 205
Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg Tyr
210 215 220
Glu Ser Lys Gln Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile Ser
225 230 235 240
Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met His Asp
245 250 255
Gly Asp Pro Gln Lys Ala Arg Leu Leu Ala Arg Phe Lys Pro Met Ala
260 265 270
Thr Leu Thr Met Lys Asn Gly Val Pro Pro Glu Lys Val Asp Val Val
275 280 285
Ser Gly Asn Ala Gln Gly Thr Gly Pro Val Gly Phe Ser Ala Ala Leu
290 295 300
Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln Arg
305 310 315 320
Val Ala Asp His Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val Leu
325 330 335
Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Thr Val
340 345 350
Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Val Ser Ser Arg
355 360 365
<210> 58
<211> 453
<212> PRT
<213> 苏云金芽孢杆菌
<400> 58
Met Asn Gly Lys Arg Asn Ile Phe Thr Cys Ile Ser Ile Ile Gly Ile
1 5 10 15
Gly Leu Ala Ser Phe Ser Ser Phe Ser Phe Ala Ala Asn Val Thr Asp
20 25 30
Asn Ser Val Gln Asn Ser Ile Pro Val Val Asn Gln Gln Val Ala Ala
35 40 45
Ala Lys Glu Met Lys Pro Phe Pro Gln Gln Val Asn Tyr Ala Gly Val
50 55 60
Ile Lys Pro Thr His Val Thr Gln Glu Ser Leu Asn Ala Ser Val Lys
65 70 75 80
Ser Tyr Tyr Asp Asn Trp Lys Lys Lys Tyr Leu Lys Asn Asp Leu Ser
85 90 95
Ser Leu Pro Gly Gly Tyr Tyr Val Lys Gly Glu Ile Thr Gly Asp Ala
100 105 110
Asp Gly Phe Lys Pro Leu Gly Thr Ser Glu Gly Gln Gly Tyr Gly Met
115 120 125
Ile Ile Thr Val Leu Met Ala Gly Tyr Asp Ser Asn Ala Gln Lys Ile
130 135 140
Tyr Asp Gly Leu Phe Lys Thr Ala Arg Thr Phe Lys Ser Ser Gln Asn
145 150 155 160
Pro Asn Leu Met Gly Trp Val Val Ala Asp Ser Lys Lys Ala Gln Gly
165 170 175
His Phe Asp Ser Ala Thr Asp Gly Asp Leu Asp Ile Ala Tyr Ser Leu
180 185 190
Leu Leu Ala His Lys Gln Trp Gly Ser Asn Gly Thr Val Asn Tyr Leu
195 200 205
Lys Glu Ala Gln Asp Met Ile Thr Lys Gly Ile Lys Ala Ser Asn Val
210 215 220
Thr Asn Asn Ser Arg Leu Asn Leu Gly Asp Trp Asp Ser Lys Asn Ser
225 230 235 240
Leu Asp Thr Arg Pro Ser Asp Trp Met Met Ser His Leu Arg Ala Phe
245 250 255
Tyr Glu Phe Thr Gly Asp Lys Thr Trp Leu Thr Val Ile Asn Asn Leu
260 265 270
Tyr Asp Val Tyr Thr Gln Phe Ser Asn Lys Tyr Ser Pro Asn Thr Gly
275 280 285
Leu Ile Ser Asp Phe Val Val Lys Asn Pro Pro Gln Pro Ala Pro Lys
290 295 300
Asp Phe Leu Glu Glu Ser Glu Tyr Thr Asn Ala Tyr Tyr Tyr Asn Ala
305 310 315 320
Ser Arg Val Pro Leu Arg Ile Val Met Asp Tyr Ala Met Tyr Gly Glu
325 330 335
Lys Arg Ser Lys Val Ile Ser Asp Lys Val Ser Ser Trp Ile Gln Asn
340 345 350
Lys Thr Asn Gly Asn Pro Ser Lys Ile Val Asp Gly Tyr Gln Leu Asn
355 360 365
Gly Ser Asn Ile Gly Ser Tyr Ser Thr Ala Val Phe Val Ser Pro Phe
370 375 380
Ile Ala Ala Ser Ile Thr Ser Ser Asn Asn Gln Lys Trp Val Asn Ser
385 390 395 400
Gly Trp Asp Trp Met Lys Asn Lys Arg Glu Ser Tyr Phe Ser Asp Ser
405 410 415
Tyr Asn Leu Leu Thr Met Leu Phe Ile Thr Gly Asn Trp Trp Lys Pro
420 425 430
Val Pro Asp Asp Lys Lys Ile Gln Asn Gln Ile Asn Asp Ala Ile Tyr
435 440 445
Glu Gly Tyr Asp Asn
450
<210> 59
<211> 377
<212> PRT
<213> 乳酸乳球菌
<400> 59
Met Glu Asn Leu Leu Leu Lys Val Ala Pro Lys Glu Glu Ile Asp Lys
1 5 10 15
Lys Leu Glu Asn Val Phe Glu Thr Leu Phe Gly Gln Asp Ser Pro Glu
20 25 30
Arg Ile Tyr Phe Glu Glu Asp Asp Met Ala Tyr Ile Val Asp Thr Gly
35 40 45
Asn Asp Asp Val Arg Thr Glu Gly Met Ser Tyr Gly Met Met Ile Ala
50 55 60
Leu Gln Leu Asp Lys Pro Lys Ile Phe Ser Gln Leu Trp Lys Trp Val
65 70 75 80
Lys Thr Tyr Met Thr Val Pro Lys Gly His Glu Asn Glu Gly Tyr Phe
85 90 95
Ile Trp Ser Cys Ala Pro Asn Gly Gln Ala Asn Ser Asp Gly Pro Ala
100 105 110
Pro Asp Gly Glu Glu Tyr Phe Ala Ala Ala Leu Leu Leu Ala Glu Lys
115 120 125
Arg Trp Lys Ile Lys Glu Tyr Gly Asp Glu Ala Arg Ala Leu Leu His
130 135 140
Ala Met Val His Lys Gly Glu Asn His Asp Gly Tyr Pro Met Phe Glu
145 150 155 160
Pro Glu Asn Thr Tyr Ile Lys Phe Val Ala His Leu His Met Thr Asp
165 170 175
Pro Ser Tyr His Leu Pro His Phe Tyr Gln Leu Tyr Ala Lys Tyr Gly
180 185 190
Asn Pro Glu Asp Ser Ala Phe Phe Leu Lys Ser Glu Glu Glu Ala Arg
195 200 205
Lys Phe Trp Leu Lys Ser Ala Asn Ala Lys Thr Gly Leu Thr Pro Glu
210 215 220
Tyr Ala Asp Tyr Glu Gly Lys Pro Tyr Asp Ile Asp Gly His Trp Thr
225 230 235 240
Phe Phe Ser Asp Ala Tyr Arg Thr Val Ala Asn Ile Gly Leu Asp Trp
245 250 255
Leu Trp Glu His Lys Glu Ile Gly Gln Ser Gln Ile Ala Leu Asn Ile
260 265 270
Gln Lys Phe Phe Glu Pro Tyr Leu Glu Asn Asp Glu Glu Ile Pro Val
275 280 285
Phe Lys Ile Asp Gly Gln Pro Leu Arg Lys Glu Glu Gln Thr Ala Glu
290 295 300
Gly Phe Pro Pro Leu Lys Val His His Pro Ile Gly Leu Trp Ser Thr
305 310 315 320
Leu Ala Gln Ala Ser Leu Val Thr Asn Glu Phe Asp Ser Thr Leu Ala
325 330 335
Leu Lys Tyr Leu Lys Tyr Phe Trp Asn Leu Lys Leu Arg Arg Gly Lys
340 345 350
Tyr Arg Tyr Tyr Asp Asn Leu Leu Tyr Leu Phe Ala Leu Leu Ala Leu
355 360 365
Ser Arg Asn Tyr Gln Lys Glu Trp Ser
370 375
<210> 60
<211> 371
<212> PRT
<213> 乳酸乳球菌
<400> 60
Met Ser Lys Arg Ile Leu Ser Phe Val Trp Phe Phe Ile Val Ile Leu
1 5 10 15
Leu Phe Ile Phe Ser Val Met Tyr Ile Lys Glu Glu Ser Ser Gln His
20 25 30
Asn Lys Gln Gln Ile Tyr Asn Gln Trp Gln Tyr Lys Tyr Ile Val Lys
35 40 45
Thr Ser Gln Gly Ser Tyr Val Asn Thr Ser Ala Asn Ser Lys Asn Arg
50 55 60
Ile Ala Leu Ser Glu Ala Gln Gly Tyr Gly Met Leu Ile Ser Val Leu
65 70 75 80
Asn Asn Gln Asp Lys Ala Ser Glu Ser Gln Phe His Asp Phe Phe Thr
85 90 95
Tyr Tyr Thr His His Arg Ile Lys Gly Thr Ser Leu Met Ser Trp Arg
100 105 110
Tyr Ile Asn Gly Ser Lys Thr Gln Lys Gln Thr Asp Leu Asn Asn Asn
115 120 125
Ala Thr Asp Gly Asp Leu Tyr Ile Ala Tyr Ala Leu Ile Leu Ala Ser
130 135 140
Glu Lys Trp Ser Gln His Lys Pro Leu Tyr Lys Thr Thr Ala Lys Gln
145 150 155 160
Ile Leu Asn Asp Ile Leu Thr Tyr Asn Val Asn His Asn Asn Asn Ile
165 170 175
Leu Thr Val Gly Asn Trp Ala Asn Ala Ser Ser Lys Tyr Gln Asn Leu
180 185 190
Met Arg Thr Ser Asp Val Leu Pro Ser Phe Phe Asp Lys Phe Tyr Lys
195 200 205
Phe Ser Asn Asn Ser Gln Trp Arg Leu Ile Lys Thr Lys Met Leu Asp
210 215 220
Ser Leu Tyr Gln Ala Ser His Lys Ser Lys Val Gly Leu Val Pro Asp
225 230 235 240
Phe Ile Gln Val Thr Asn Gly Asn Lys Val His Ser Leu Ser Gln Asp
245 250 255
Lys Asn Leu Lys Leu Asn Lys His Asp Asp Asp Tyr Tyr Tyr Asn Ala
260 265 270
Phe Arg Val Pro Tyr Asn Leu Ala Ile Asn Lys Asn Lys Ser Ser Lys
275 280 285
Glu Lys Gln Val Leu Thr Glu Met Met Lys Phe Phe Ser Ser Leu Gln
290 295 300
Lys Ile Ser Gly Gly Tyr Thr Met Ser Gly Lys Pro Leu Asn Lys Phe
305 310 315 320
Gln Ser Thr Ser Ile Ser Ala Pro Ile Phe Tyr Ala Ser Ser Ile Ser
325 330 335
Gln Arg Trp Gln Thr Leu His Arg Glu Gln Ser Phe Val Leu Asp Tyr
340 345 350
Gly Ser Leu Asn His Asn Tyr Tyr Asp Asp Thr Leu Leu Val Leu Val
355 360 365
Leu Phe Ser
370
<210> 61
<211> 419
<212> PRT
<213> 扭脱甲基杆菌
<400> 61
Met Arg Pro Pro Arg Thr Pro Arg Arg Ile Gly Trp Ala Phe Ala Ser
1 5 10 15
Ala Leu Ala Leu Ala Ala Ala Gly Ser Ala Ala Ala Gln Thr Thr Pro
20 25 30
Gln Pro Ala Ser Pro Gln Pro Ala Ser Pro Glu Thr Ser Ser Gln Met
35 40 45
Pro Gln Pro Ala Pro Gln Pro Ala Pro Gln Gln Thr Glu Ala Thr Thr
50 55 60
Val Pro Pro Ala Thr Ala Leu Pro Gly Pro Pro Arg Ala Glu Thr Ser
65 70 75 80
Ala Arg Thr Asp Ser Gly Pro Leu Leu Ala Asn Thr Leu Gly Asp Asp
85 90 95
Ala Ala Trp Arg Ala Tyr Arg Ser Arg Phe Ile Thr Glu Gln Gly Arg
100 105 110
Ile Val Asp Thr Ala Asn Gly Leu Ile Ser His Ser Glu Gly Gln Gly
115 120 125
Tyr Gly Met Leu Leu Ala Val Ala Ala Gly Asp Arg Ser Thr Phe Glu
130 135 140
Arg Ile Trp Gly Trp Thr Arg Ala Asn Leu Met Val Arg Ser Asp Glu
145 150 155 160
Leu Leu Ala Trp Arg Trp Ala Pro Asp His Arg Pro Ala Val Ser Asp
165 170 175
Met Asn Asn Ala Thr Asp Gly Asp Ile Leu Val Ala Trp Ala Leu Thr
180 185 190
Glu Ala Ala Glu Ala Trp Gly Glu Pro Ser Tyr Arg Thr Ala Ala Arg
195 200 205
Arg Ile Ala Val Glu Phe Gly Arg Lys Thr Ile Leu Phe Lys Asp Pro
210 215 220
His Gly Pro Val Leu Leu Pro Ala Val Ser Gly Phe Ser Ala Arg Glu
225 230 235 240
Arg Ala Asp Gly Pro Leu Ile Asn Leu Ser Tyr Trp Val Phe Pro Ala
245 250 255
Phe Gln Arg Leu Pro Ile Val Ala Pro Glu Tyr Asp Trp Ala Ser Leu
260 265 270
Ile Arg Ser Gly Val Asp Phe Leu Arg Gln Ser Arg Phe Gly Pro Ser
275 280 285
Ser Leu Pro Thr Glu Trp Ile Ser Ala Lys Asp Ser Leu Arg Pro Ala
290 295 300
Asp Gly Phe Pro Pro Leu Phe Ser Tyr Asn Ala Ile Arg Val Pro Leu
305 310 315 320
Tyr Leu Ala Trp Ala Gly Val Gly Arg Pro Glu Asp Tyr Ala Pro Phe
325 330 335
Lys Thr Leu Trp Gly Gly Ile Glu Arg Glu Arg Leu Pro Ile Val Asp
340 345 350
Thr Arg Asp Gly Gln Pro Val Glu Trp Leu Ser Glu Pro Gly Tyr Met
355 360 365
Ala Ile Ser Ala Ile Thr Ala Cys Ala Ala Asp Gly Thr Pro Phe Pro
370 375 380
Glu Ala Leu Arg Thr Val Gln Asp Asn Gln Asn Tyr Tyr Pro Ala Thr
385 390 395 400
Leu Gln Leu Leu Ser Leu Ile Ala Ala Arg Met Arg Tyr Pro Ser Cys
405 410 415
Val Lys Ser
<210> 62
<211> 371
<212> PRT
<213> 恶臭假单胞菌
<400> 62
Met Met Arg Gln Met Leu Val Gly Leu Val Ala Leu Gly Leu Pro Val
1 5 10 15
Phe Ala Asn Ala Glu Ala Ala Cys Ser Trp Pro Ala Trp Glu Arg Phe
20 25 30
Lys Ala Glu Leu Val Ser Ala Asp Gly Arg Val Ile Asp Pro Ser Asp
35 40 45
Ala Arg Leu Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Val Gly Asn Asp Arg Asp Ala Phe Ala Gln Leu Leu Arg Trp
65 70 75 80
Thr Gly Asn Asn Leu Ala Glu Gly Asp Leu Ala Arg His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Arg Asn Glu Gln Gln Gln Trp Gln Val Leu Asp Ala
100 105 110
Asn Asn Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Gln Gln Pro Ala Tyr Thr Gln Leu Gly Gln Arg
130 135 140
Leu Leu Trp Arg Ile Ala Ala Gln Thr Val Arg Lys Leu Pro Gly Leu
145 150 155 160
Gly Val Met Leu Leu Pro Gly Asp Tyr Gly Phe Glu Asp Ala Gln Gly
165 170 175
Thr Arg Leu Asn Pro Ser Tyr Leu Pro Leu Gln Leu Phe Asp Arg Phe
180 185 190
Ser Glu Val Asp Pro Leu Trp Gly Glu Leu Ala Ala Asn Thr Arg Arg
195 200 205
Leu Trp Leu Ala Ser Ser Pro Lys Gly Phe Ala Pro Asp Trp Leu Leu
210 215 220
Trp Thr Pro Ala Gly Lys Pro Ala Ala Asp Pro Gln His Gly Ser Ala
225 230 235 240
Gly Asp Tyr Asp Ala Ile Arg Val Tyr Leu Trp Val Gly Met Leu Ala
245 250 255
Lys Asp Ala Val Gln Arg Asn Glu Leu Val Ala His Tyr Ala Pro Met
260 265 270
Ala Ala Leu Thr Gln Arg Gln Gly Leu Pro Pro Glu Arg Ala Asp Ala
275 280 285
Arg Ser Gly Glu Ala Ser Gly Gln Gly Pro Ala Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Leu Leu Ala Ala Ser Pro Ala His Val Ala Gly Leu Ala
305 310 315 320
Ala Gln Arg Gln Arg Leu Arg Asp Gln Pro Val Glu Ala Lys Ala Tyr
325 330 335
Tyr Ser Gln Val Leu Val Leu Phe Gly Gln Gly Trp Asp Glu Ala Arg
340 345 350
Tyr Arg Phe Asp Pro His Gly Arg Leu Leu Pro Ala Trp Ser Ala Pro
355 360 365
Cys Asn Glu
370
<210> 63
<211> 393
<212> PRT
<213> 甲基杆菌属菌种4-46
<400> 63
Met Arg Ser Phe Leu Arg Leu Ala Ala Thr Ile Leu Ile Leu Gly Ser
1 5 10 15
Ala Ala Leu Gly Gly Ala Arg Pro Gly Leu Ala Gln Gln Pro Gly Ala
20 25 30
Ala Glu Pro Pro Pro Arg Ala Arg Asp Ala Lys Asn Arg Asp Gly Glu
35 40 45
Ser Arg Thr Gly Glu Asn Arg Thr Gly Glu Pro Ala Ser Leu His Asn
50 55 60
Ala Leu Gly Asn Gly Ala Ala Trp Arg Ala Tyr Lys Ala Arg Phe Val
65 70 75 80
Thr Asp Gln Gly Arg Val Val Asp Thr Ala Asn Gly Arg Ile Ser His
85 90 95
Ser Glu Gly Gln Gly Tyr Gly Met Leu Leu Ala Val Ala Ala Gly Asp
100 105 110
Arg Asp Ala Phe Gln Arg Ile Trp Asp Trp Thr Arg Ala Asn Leu Met
115 120 125
Val Arg Asp Asp Ser Leu Leu Ala Trp Arg Trp Glu Pro Asp Lys Arg
130 135 140
Pro Gly Val Ala Asp Met Asn Asn Ala Thr Asp Gly Asp Leu Leu Val
145 150 155 160
Ala Trp Ala Leu Ile Glu Ala Ala Asp Ala Trp Gln Asp Glu Gly Tyr
165 170 175
Arg Leu Ala Ala Arg Arg Ile Ala Val Asp Ile Gly Arg Arg Thr Val
180 185 190
Leu Phe Arg Ser Glu Gly Ala Ala Leu Leu Leu Pro Gly Met Ala Gly
195 200 205
Phe Ser Ala Glu Asp Arg Ala Asp Gly Pro Val Ile Asn Leu Ser Tyr
210 215 220
Trp Ile Phe Pro Ala Leu Ala Arg Leu Pro Ala Val Ala Pro Glu Phe
225 230 235 240
Asp Trp Ala Arg Leu Ser Ala Ala Gly Leu Asp Leu Ala Leu Arg Ala
245 250 255
Arg Phe Gly Glu Ala Ala Leu Pro Val Glu Trp Thr Ser Leu Arg Gly
260 265 270
Gly Glu Pro Lys Ala Ala Ala Gly Phe Pro Pro Val Phe Ser Tyr Asn
275 280 285
Ala Val Arg Val Pro Leu Tyr Leu Ala Met Ala Gly Ile Ala Glu Arg
290 295 300
Arg Tyr Tyr Ala Pro Phe Val Lys Ala Trp Ala Glu Val Gly Ala Ser
305 310 315 320
Gly Leu Pro Val Val Asp Thr Ala Ser Asn Asp Val Val Gly Arg Met
325 330 335
Gln Glu Pro Gly Tyr Leu Ala Val Ala Ala Leu Thr Ala Cys Ala Ala
340 345 350
Gly Thr Ala Ser Leu Pro Pro Ala Leu Pro Asp Pro Thr Ser Pro Gln
355 360 365
Asn Tyr Tyr Pro Ala Thr Leu Gln Leu Leu Ala Leu Ala Ala Val Asn
370 375 380
Met Arg Tyr Ala Ser Cys Leu Gly Arg
385 390
<210> 64
<211> 369
<212> PRT
<213> Escherichia albertii
<400> 64
Met Met Asn Val Leu Arg Asn Gly Ile Val Thr Met Leu Leu Gln Ala
1 5 10 15
Ala Phe Ser Val Gln Ala Ala Cys Asn Trp Pro Ala Trp Glu Gln Phe
20 25 30
Lys Lys Asp Tyr Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser Asp
35 40 45
Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe Phe
50 55 60
Ala Leu Ala Ala Asn Asp Arg Ala Ala Phe Asp Asn Leu Leu Asp Trp
65 70 75 80
Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Lys Glu His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Lys Lys Glu Asn Thr Lys Trp Glu Val Leu Asp Ser
100 105 110
Asn Ser Ala Ser Asp Gly Asp Ile Trp Met Ala Trp Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser Ala
130 135 140
Leu Leu Lys Arg Ile Ala Gln Glu Glu Val Val Thr Val Pro Gly Leu
145 150 155 160
Gly Ser Met Leu Leu Pro Gly Lys Val Gly Phe Val Glu Asp Asn Thr
165 170 175
Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Thr Leu Ala His Tyr Phe
180 185 190
Thr Arg Phe Gly Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln Arg
195 200 205
Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg
210 215 220
Tyr Glu Lys Asp Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile
225 230 235 240
Ser Ser Tyr Asp Ala Ile Arg Val Tyr Met Trp Val Gly Met Met Pro
245 250 255
Asp Ser Asp Pro Gln Lys Ala Arg Leu Leu Ser Arg Phe Lys Pro Met
260 265 270
Ala Thr Phe Thr Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp Val
275 280 285
Ala Met Gly Lys Ala Gln Gly Thr Gly Pro Glu Gly Phe Ser Ala Ala
290 295 300
Met Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln
305 310 315 320
Arg Val Ala Asp Asn Leu Pro Gly Ser Asp Ala Tyr Tyr Asn Ser Val
325 330 335
Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Ser
340 345 350
Thr Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn Ser
355 360 365
His
<210> 65
<211> 476
<212> PRT
<213> Burkholderia graminis
<400> 65
Met Val Lys Arg Thr Val Gln Val Arg Ala Asn Gly Val Gln Ser Pro
1 5 10 15
Ser Val Gly Val Ala Arg Ala Ala Cys Ala Arg Asn Ala Arg Arg Val
20 25 30
Ala Ser Gly Val Cys Gly Ala Val Leu Ala Trp Leu Ala Ala Gly Val
35 40 45
Ala Leu Ala Pro Ala Ser Val Ala Ala Ala Ala Ser Ala Ala Ser Ser
50 55 60
Ala Ala Asn Ser Ala Ala Ser Ser Ala Ala Ala Ser Ala Gly Thr Ser
65 70 75 80
Ala Thr Ser Ser Ala Ala Thr Ser Ala Thr Gly Ala Thr Gly Thr Ala
85 90 95
Ala Cys Pro Ala Gln Ala Phe Arg Ala Phe Asp Asn Ala Gly Gly Ala
100 105 110
Gln Ser Thr Ala Leu Ala Val Ser Ala Asp Trp Pro Ala Trp Glu Gln
115 120 125
Phe Arg Arg Lys Phe Val Ser Pro Asp Gly Arg Val Ile Asp Val Gly
130 135 140
Ser Asp Asp Asp Arg Thr Val Ser Glu Gly Gln Ser Tyr Gly Leu Phe
145 150 155 160
Phe Ala Leu Val Ala Asn Asp Arg Ala Ser Phe Asp Arg Leu Leu His
165 170 175
Trp Thr Glu Asn Asn Leu Ala Ala Gly Asp Leu Thr Ala His Leu Pro
180 185 190
Ala Trp Leu Trp Gly Arg Gly Pro Asp Gly Lys Trp Gly Val Leu Asp
195 200 205
Ser Asn Ala Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ala Leu Leu
210 215 220
Glu Ala Gly Arg Val Trp Asn Glu Arg Ser Tyr Thr Ala Arg Gly Ala
225 230 235 240
Ala Leu Ala Arg Arg Val Leu Asp Glu Glu Thr Ala Arg Val Pro Thr
245 250 255
Leu Gly Leu Thr Leu Leu Pro Gly Pro Thr Gly Phe His Pro Glu Ala
260 265 270
Asp Leu Trp Arg Val Asn Pro Ser Tyr Ala Pro Val Gln Val Leu Arg
275 280 285
Gly Leu Ala Ser Ala Leu Pro Ser Asp Arg Arg Trp Gln Ala Leu Val
290 295 300
Asp Ser Ser Ala Arg Met Leu Val Asp Ser Ala Pro Gln Gly Phe Ser
305 310 315 320
Pro Asp Trp Ser Leu Tyr Arg Ala Gly Lys Gly Phe Leu Pro Asp Pro
325 330 335
Ala Thr His Ala Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp
340 345 350
Ile Gly Met Leu Asn Gly Ser Asp Pro Leu Lys Ser Arg Leu Leu Asn
355 360 365
His Phe Thr Pro Phe Ala Arg Tyr Ile Ala Glu His Gly Ala Pro Pro
370 375 380
Glu Arg Ile Asp Thr Thr Asp Ala Lys Pro Gly Ala Asn Asp Gly Asn
385 390 395 400
Ala Gly Phe Ser Ala Ala Ala Ile Pro Phe Leu Ile Ala Arg Gly Glu
405 410 415
Thr Ser Ile Ala Asp Arg Gln Ser Ala Arg Val Asp Gln Leu Asp Ala
420 425 430
Gln Thr Ala Pro Gly Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu
435 440 445
Gly Trp Arg Asp Gly Arg Tyr Arg Phe Ala Ala Asp Gly Ser Leu Asp
450 455 460
Leu Pro Trp Ser Ser Val Cys Arg Pro Ala Lys Arg
465 470 475
<210> 66
<211> 513
<212> PRT
<213> 类鼻疽伯克霍尔德氏菌
<400> 66
Met Ala Arg Gln Ala Gly Arg Asp Glu Gly Val Gly Arg Ile Gly Arg
1 5 10 15
Ile Gly Arg Glu Ala Gly Asp Ala Arg Glu Ala Ala Gly Ala Gly Glu
20 25 30
Ala Cys Gly Arg Arg His Ala Arg Ala Asp Thr Trp Ala Arg Arg Ala
35 40 45
Ser Arg Ala Ser Arg Gly Trp Arg Thr Phe Trp Arg Gly Arg Gly Gly
50 55 60
Val Arg Ala Val Val Ala Ser Val Val Ala Ser Phe Ser Val Met Ala
65 70 75 80
Phe Ala Ala Ala Thr Leu Pro Val Ser Trp Arg Val Ala Ala Ala Ala
85 90 95
Glu Arg Thr Arg Ser Gly Gly Glu Arg Ala Gly Gly Leu Arg Asp Thr
100 105 110
Ala Gly Leu Ile Glu Ile Ser Ala Ala Ala Pro Ala Ser Thr Pro Ile
115 120 125
Pro Ala Ala Pro Arg Arg Phe Ala Gln Pro Phe Ala Gln Thr Phe Ala
130 135 140
Gln Pro Ala Arg Ala Phe Ala Val Ala Ser Ala Cys Ala Pro Ser Trp
145 150 155 160
Pro Arg Trp Asp Arg Phe Lys Arg Asp Phe Val Ser Ala Asp Gly Arg
165 170 175
Val Ile Asp Val Gly Ser Ala Asp Glu Arg Thr Val Ser Glu Gly Gln
180 185 190
Ala Tyr Gly Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Ala Ala Phe
195 200 205
Asp Ala Leu Leu Arg Trp Thr Glu Asp Asn Leu Ala Gln Gly Asp Leu
210 215 220
Ser Ala Arg Leu Pro Ala Trp Leu Trp Gly Arg Ala Ala Asp Gly Ala
225 230 235 240
Trp Arg Val Leu Asp Ala Asn Ala Ala Ser Asp Ala Asp Leu Trp Leu
245 250 255
Ala Tyr Ala Leu Leu Glu Ala Gly Arg Leu Trp Arg Glu Arg Ser Tyr
260 265 270
Thr Ala Arg Gly Ala Leu Leu Ala Lys Arg Val Leu Asp Glu Glu Thr
275 280 285
Ala Thr Leu Pro Gly Leu Gly Leu Val Leu Leu Pro Gly Pro Thr Gly
290 295 300
Phe Arg Pro Ala Arg Asp Ala Trp Arg Leu Asn Pro Ser Tyr Ser Pro
305 310 315 320
Pro Gln Ala Ile Arg Gly Ile Gly Ala His Val Pro Asp Asp Ala Arg
325 330 335
Trp Ala Arg Leu Ala Ala Gly Val Gly Arg Val Leu Thr Asp Ser Ala
340 345 350
Pro Arg Gly Phe Ala Pro Asp Trp Ala Leu Tyr Arg Ala Gly Arg Gly
355 360 365
Phe Glu Pro Asp Ala Glu Thr His Ala Val Ser Ala Tyr Asn Ala Ile
370 375 380
Arg Val Tyr Leu Trp Ala Gly Met Leu Asp Ala Gly Asp Pro Leu Ala
385 390 395 400
Arg Pro Leu Val Ala His Phe Ala Pro Phe Ala Glu His Val Ala Ala
405 410 415
His Gly Ala Pro Pro Glu Ala Val Asp Ala Thr Thr Gly Ala Ala Ala
420 425 430
Pro Arg Asp Gly Asn Ala Gly Phe Ser Ala Ala Ala Val Pro Phe Leu
435 440 445
Glu Ala Arg Gly Glu Arg Ala Ser Ala Asp Ala Gln Leu Ala Arg Val
450 455 460
Ala Arg Leu Glu Arg Glu Thr Ala Ser Gly Tyr Tyr Ala Asn Val Leu
465 470 475 480
Thr Leu Phe Gly Leu Gly Trp Arg Asp Gly Arg Tyr Arg Phe Ala Ala
485 490 495
Asp Gly Thr Leu Arg Val Arg Trp Ser Glu Pro Cys Ser Thr Pro Ala
500 505 510
Arg
<210> 67
<211> 371
<212> PRT
<213> 假结核耶尔森氏菌
<400> 67
Met Val Val Met Phe Lys His Leu Ala Ser Met Phe Leu Leu Leu Ala
1 5 10 15
Ser Phe Ser Leu Ala Ala Thr Ser Asn Trp Pro Ala Trp Gln Gln Phe
20 25 30
Lys Gln Asp Tyr Ile Ser Glu Gly Gly Arg Ile Ile Asp Pro Gly Ser
35 40 45
Pro Ser Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe
50 55 60
Ala Leu Val Ala Asp Asp Gln Pro Met Phe Glu Arg Leu Leu Ala Trp
65 70 75 80
Thr Glu Asn Asn Leu Ala Ala Gly Asp Leu Thr Ser Arg Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Asn Ser Gln Asn Asn Trp Asp Ile Leu Asp Pro
100 105 110
Asn Ser Ala Ser Asp Ala Asp Ile Leu Ile Ala Tyr Asn Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Gly Asn Arg Arg Tyr Leu Ile Met Gly Thr Leu
130 135 140
Leu Leu Gln Arg Ile Ala Gln Glu Glu Val Met Asp Ile Pro Gly Leu
145 150 155 160
Gly Gln Met Leu Leu Pro Gly Lys Ile Gly Phe Asn Asp Glu Asp Thr
165 170 175
Trp Arg Leu Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala Arg Phe
180 185 190
Ser Ser Ile Asp Gly Pro Trp Glu Ala Met Val Glu Val Asn Gln Arg
195 200 205
Met Trp Leu Glu Thr Ala Pro Asn Gly Phe Ser Pro Asp Trp Val Val
210 215 220
Trp Gln Lys Gly Lys Gly Trp Gln Pro Asp Thr Ile Lys Pro Asp Val
225 230 235 240
Gly Ser Asn Asp Ala Ile Leu Val Tyr Leu Trp Ala Gly Met Leu Ala
245 250 255
Met Asp Ser Pro Gln Lys Ala Glu Leu Ile Ala Arg Phe Gln Pro Met
260 265 270
Ala Val Ile Thr Gln Gln Gln Gly Leu Pro Pro Phe Thr Thr Asn Ser
275 280 285
Asp Asn Gly Lys Thr Asn Gly Asp Gly Ser Val Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Phe Leu Ala Ser Ser Pro Glu Pro Phe Asn Gln Gln Thr
305 310 315 320
Leu Asn Leu Gln Gln Arg Arg Val Gln Asn Ser Pro Pro Gly Ala Asp
325 330 335
Asp Tyr Tyr Ser Ala Ile Leu Thr Leu Phe Gly Gln Gly Trp Leu Gln
340 345 350
His Arg Tyr His Phe Thr His Gln Gly Glu Leu Gln Pro Ser Trp His
355 360 365
Arg Gln Arg
370
<210> 68
<211> 409
<212> PRT
<213> Burkholderia cenocepacia
<400> 68
Met Ala Arg Ala Met Thr Lys Arg Arg Ala Met Gln Pro Ala Arg Arg
1 5 10 15
Val Gly Ala Thr Leu Ala Leu Ala Val Ala Val Thr Cys Ala Ala Ala
20 25 30
Gly Met Ala Thr Arg Ala His Ala Ala Ala Ala Gly Ala Ala Asp Ala
35 40 45
Ala Ser Ala Gly Cys Ser Ala Ala Trp Pro Arg Trp Asp Ala Phe Lys
50 55 60
Arg Asp Phe Ile Ser Ala Asp Gly Arg Val Ile Asp Val Gly Ser Ala
65 70 75 80
Asp Ser Arg Thr Val Ser Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala
85 90 95
Leu Val Ala Asn Asp Arg Arg Met Phe Asp Thr Ile Leu Ala Trp Thr
100 105 110
Glu Asn Asn Leu Ala Gln Gly Asp Leu Ser Ala His Leu Pro Ala Trp
115 120 125
Leu Trp Gly Arg Ala Pro Asp Gly Ala Trp Arg Val Leu Asp Ala Asn
130 135 140
Pro Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ala Leu Val Glu Ala
145 150 155 160
Gly Arg Leu Trp His Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu
165 170 175
Ala Lys Arg Val Leu Asp Ala Glu Thr Ala Thr Val Pro Gly Leu Gly
180 185 190
Leu Thr Leu Leu Pro Gly Pro Thr Gly Phe Lys Leu Ala Asn Gly Gln
195 200 205
Trp Arg Val Asn Pro Ser Tyr Ser Pro Pro Gln Val Ile Arg Ala Leu
210 215 220
Gly Ala Arg Leu Pro Asp Asp Arg Arg Trp Ala Ala Leu Ala Ser Ser
225 230 235 240
Thr Ala Arg Val Leu Leu Asp Thr Ala Pro Lys Gly Phe Ser Pro Asp
245 250 255
Trp Ala Leu Tyr Arg Ala Gly Asn Gly Phe Gly Pro Asp Pro Gln Thr
260 265 270
His Ala Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly
275 280 285
Met Leu Asp Arg Ala Asp Pro Leu Ala Ala Pro Leu Leu Ala Lys Phe
290 295 300
Ala Pro Phe Ala Asp His Val Ala Ala His Gly Ala Pro Pro Glu Lys
305 310 315 320
Val Asp Thr Thr Thr Gly Val Ala Gly Pro Asn Asp Gly Asn Gly Gly
325 330 335
Phe Ser Ala Ala Ala Val Pro Phe Leu Asp Ala Arg Gly Gln His Ala
340 345 350
Leu Ala Asp Ala Gln Ala Ala Arg Val Asp Thr Leu Ala Arg Gln Ser
355 360 365
Ala Pro Gly Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu Gly Trp
370 375 380
Arg Asp Gly Arg Tyr Arg Phe Gly Ala Asp Gly Thr Leu Asp Thr Arg
385 390 395 400
Trp Gly Gly Arg Ser Cys Ala Ala Arg
405
<210> 69
<211> 370
<212> PRT
<213> 大肠杆菌
<400> 69
Met Lys Met Asn Val Leu Arg Ser Gly Leu Val Thr Met Leu Leu Leu
1 5 10 15
Ala Ala Phe Ser Val Gln Ala Ala Cys Asn Trp Pro Ala Trp Glu Gln
20 25 30
Phe Lys Lys Asp Tyr Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser
35 40 45
Asp Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe
50 55 60
Phe Ala Leu Ala Ala Asn Asp Arg Ala Ala Phe Asp Asn Ile Leu Asp
65 70 75 80
Trp Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Lys Glu Arg Leu Pro
85 90 95
Ala Trp Leu Trp Gly Lys Lys Glu Asn Ser Lys Trp Glu Val Leu Asp
100 105 110
Ser Asn Ser Ala Ser Asp Gly Asp Val Trp Met Ala Trp Ser Leu Leu
115 120 125
Glu Ala Gly Arg Leu Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser
130 135 140
Ala Leu Leu Lys Arg Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly
145 150 155 160
Leu Gly Ser Met Leu Leu Pro Gly Lys Val Gly Phe Ala Glu Asp Asn
165 170 175
Ser Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Thr Leu Ala Gln Tyr
180 185 190
Phe Thr Arg Phe Gly Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln
195 200 205
Arg Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val
210 215 220
Arg Tyr Glu Lys Asp Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu
225 230 235 240
Ile Ser Ser Tyr Asp Ala Ile Arg Val Tyr Met Trp Val Gly Met Met
245 250 255
Pro Asp Ser Asp Pro Gln Lys Ala Arg Met Leu Asn Arg Phe Lys Pro
260 265 270
Met Ala Thr Phe Thr Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp
275 280 285
Val Ala Thr Gly Lys Ala Gln Gly Lys Gly Pro Val Gly Phe Ser Ala
290 295 300
Ala Met Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg
305 310 315 320
Gln Arg Val Ala Asp Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr
325 330 335
Val Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe
340 345 350
Ser Thr Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn
355 360 365
Ser His
370
<210> 70
<211> 384
<212> PRT
<213> 耐辐射甲基杆菌
<400> 70
Met Ser Arg Arg Asp Arg Ala Pro Leu Ala Ala Ala Phe Leu Val Ala
1 5 10 15
Ala Met Leu Pro Ala Ala Ala Ala Ser Thr Gln Asp Glu Pro Ala Val
20 25 30
Pro Pro Ser Thr Ala Gln Pro Gly Thr Gln Pro Ala Gln Thr Ala Arg
35 40 45
Ala Ala Ser Pro Gly Leu Ala Gly Lys Leu Gly Asn Asp Ala Ala Trp
50 55 60
Arg Ala Tyr Arg Ser Arg Phe Ile Thr Asp Gln Gly Arg Val Val Asp
65 70 75 80
Thr Ala Asn Gly Leu Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met
85 90 95
Leu Leu Ala Val Ala Ala Gly Asp Arg Val Ser Phe Glu Arg Ile Trp
100 105 110
Gly Trp Thr Arg Ala Asn Leu Met Val Arg Gly Asp Glu Leu Leu Ala
115 120 125
Trp Arg Trp Ser Pro Asp Lys Arg Pro Ala Val Ser Asp Met Asn Asn
130 135 140
Ala Thr Asp Gly Asp Ile Leu Val Ala Trp Ala Leu Thr Glu Ala Ala
145 150 155 160
Glu Ala Trp Asn Glu Pro Ser Tyr Arg Thr Ala Ala Arg Arg Ile Ala
165 170 175
Ile Glu Phe Gly Arg Lys Thr Ile Leu Phe Arg Asp Pro His Gly Ala
180 185 190
Leu Leu Leu Pro Ala Val Ser Gly Phe Ser Ala Arg Glu Arg Pro Asp
195 200 205
Gly Pro Leu Val Asn Leu Ser Tyr Trp Ile Phe Pro Ala Phe Pro Arg
210 215 220
Leu Ala Leu Val Ala Pro Glu Tyr Asp Trp Ala Ala Leu Thr Arg Ser
225 230 235 240
Gly Leu Ser Leu Leu Arg Gln Ser Arg Phe Gly Pro Ser Asn Leu Pro
245 250 255
Thr Glu Trp Ile Ser Ala Lys Asp Ala Pro His Pro Ala Thr Gly Phe
260 265 270
Pro Pro Leu Phe Ser Tyr Asn Ala Ile Arg Val Pro Leu Tyr Leu Ala
275 280 285
Trp Ala Gly Leu Val Arg Pro Asp Asp Leu Ala Pro Phe Gln Ala Leu
290 295 300
Trp Ser Ser Ser Asp Arg Glu Arg Leu Pro Ile Val Asp Thr Gly Asp
305 310 315 320
Gly Arg Lys Val Glu Trp Leu Thr Glu Ala Gly Tyr Met Gly Ile Pro
325 330 335
Ala Leu Val Ala Cys Ala Leu Asp Gly Thr Pro Phe Pro Glu Asp Ala
340 345 350
Arg Gly Asp Ile Gly Asn Gln Asn Tyr Tyr Pro Ala Thr Leu Ser Leu
355 360 365
Leu Ala Leu Thr Ala Ala Arg Met Arg Tyr Pro Ser Cys Leu Lys Ser
370 375 380
<210> 71
<211> 367
<212> PRT
<213> 耐辐射甲基杆菌
<400> 71
Met Ser Val Leu Ile Ser Val Leu Leu Arg Gly Leu Ala Gly Leu Cys
1 5 10 15
Val Leu Thr Leu Leu Ala Ala Arg Pro Ala Leu Ala Ala Thr Ala Ser
20 25 30
Ala Pro Pro Leu Ala Gly Thr Leu Arg Asn Ala Glu Ala Trp Arg Ala
35 40 45
Tyr Lys Ala Arg Phe Val Ser Glu Gln Gly Arg Val Val Asp Thr Ala
50 55 60
Asn Gly Gly Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu Leu
65 70 75 80
Ala Val Ala Ala Gly Asp Arg Glu Thr Phe Glu Arg Ile Trp Thr Trp
85 90 95
Thr Arg Ala Asn Leu Met Val Arg Asp Asp Gln Leu Ile Ala Trp Arg
100 105 110
Trp Glu Pro Asp Lys Arg Pro Ala Val Ala Asp Met Asn Asp Ala Ser
115 120 125
Asp Gly Asp Leu Leu Val Ala Trp Ala Leu Thr Glu Ala Ala Glu Ala
130 135 140
Trp His Glu Pro Glu His Gln Val Ala Gly Arg Arg Ile Ala Val Glu
145 150 155 160
Leu Gly Arg Lys Leu Leu Leu Pro Arg Ala Pro Gln Gly Pro Leu Ile
165 170 175
Leu Pro Ala Val Ala Gly Phe Ser Ala Glu Asp Arg Ala Asp Gly Pro
180 185 190
Val Val Asn Leu Ser Tyr Trp Val Phe Pro Ala Leu Asp Arg Val Gly
195 200 205
Leu Leu Ala Pro Glu Phe Asp Trp Arg Ala Leu Ala Arg Ser Gly Arg
210 215 220
Arg Leu Ile Ala Ala Ala Arg Phe Gly Ser Gln Arg Leu Pro Val Glu
225 230 235 240
Trp Ile Ser Leu Ser Gly Ala Ala Pro Arg Pro Ala Glu Gly Phe Pro
245 250 255
Ala Glu Phe Gly Tyr Asn Ala Leu Arg Ile Pro Leu Tyr Leu Ala Met
260 265 270
Ala Gly Ile Thr Glu Pro Asp Leu Tyr Ala Pro Phe Leu Ala Leu Trp
275 280 285
Gln Lys Pro Glu Ala Gly Leu Ala Leu Val Glu Thr Gly Thr Gly Arg
290 295 300
Val Ala Ala Arg Leu Thr Glu Thr Gly Tyr Ala Ala Ile Pro Ala Leu
305 310 315 320
Ala Ala Cys Ala Ser Arg Gly Ala Pro Phe Pro Ala Ala Leu Thr Arg
325 330 335
Val Arg Ala Glu Ser Glu Asn Tyr Tyr Pro Val Thr Leu Gln Leu Leu
340 345 350
Ala Leu Ala Ala Leu Asn Ala Arg Tyr Pro Ala Cys Ala Pro Arg
355 360 365
<210> 72
<211> 359
<212> PRT
<213> Burkholderia ambifaria
<400> 72
Met Gln Cys Gly Ala Ala Trp Pro Arg Trp Asp Ala Phe Lys Arg Asp
1 5 10 15
Phe Val Ser Ala Asp Gly Arg Val Ile Asp Val Gly Ser Ala Asp Ser
20 25 30
Arg Thr Val Ser Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val
35 40 45
Ala Asn Asp Arg Arg Thr Phe Asp Thr Ile Leu Ala Trp Thr Glu Asn
50 55 60
Asn Leu Ala Gln Gly Asp Leu Ser Ala Arg Leu Pro Ala Trp Leu Trp
65 70 75 80
Gly Arg Ala Pro Asp Gly Ala Trp Arg Val Leu Asp Ala Asn Ala Ala
85 90 95
Ser Asp Ala Asp Leu Trp Ile Ala Tyr Thr Leu Val Glu Ala Gly Arg
100 105 110
Leu Trp Arg Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu Ala Lys
115 120 125
Arg Val Leu Asp Asp Glu Thr Ala Ser Val Pro Gly Leu Gly Leu Thr
130 135 140
Leu Leu Pro Gly Pro Thr Gly Phe Lys Arg Ala Asn Gly Gln Trp Arg
145 150 155 160
Val Asn Pro Ser Tyr Ser Pro Pro Gln Val Ile Arg Gly Leu Ala Ala
165 170 175
Arg Leu Pro Asp Asp Arg Arg Trp Ala Ala Leu Ala Ala Ser Thr Gly
180 185 190
Arg Val Leu Leu Asp Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Ala
195 200 205
Leu Tyr Arg Ala Gly Ala Gly Phe Gly Pro Asp Pro Gln Thr Arg Ala
210 215 220
Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Leu
225 230 235 240
Asp Arg Ala Asp Pro Leu Ala Ala Pro Leu Leu Ala Arg Phe Ala Pro
245 250 255
Phe Ala Asp Tyr Ile Ala Ala His Gly Ala Pro Pro Glu Lys Val Asp
260 265 270
Thr Thr Thr Gly Val Ala Gly Pro Asn Asp Gly Asn Gly Gly Phe Ser
275 280 285
Ala Ala Ala Val Pro Phe Leu Asp Ala Arg Gly Gln His Ala Leu Ala
290 295 300
Asp Ala Gln Ala Ala Arg Val Asp Thr Leu Ala Arg Gln Ser Ala Pro
305 310 315 320
Gly Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu Gly Trp Arg Asp
325 330 335
Gly Arg Tyr Arg Phe Gly Ala Asp Gly Ala Leu Asp Ala Arg Trp Glu
340 345 350
Gly Arg Pro Cys Ala Ala Arg
355
<210> 73
<211> 379
<212> PRT
<213> 霍氏纤发菌
<400> 73
Met Ala Thr Arg Ala Gln Ser Ala Asn Pro Cys Ser Ala Val Glu Gly
1 5 10 15
Pro Thr Gly Trp Pro Ala Trp Gln Thr Leu Arg Arg Thr Leu Met Ser
20 25 30
Arg Asp Gly Arg Val Ile Asp Arg Tyr Ala Ser Asp Ala Thr Thr Ser
35 40 45
Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val Asp Asn Asp Arg
50 55 60
Ala Ala Phe Glu Leu Leu Leu Arg Trp Thr Glu Asp Asn Leu Ala Ala
65 70 75 80
Gly Asp Leu Ala Ala Arg Leu Pro Ala Trp Arg Trp Gly Arg Arg Ala
85 90 95
Asp Gly Ser Trp Asn Val Ile Asp Ala Asn Ser Ala Ala Asp Ala Asp
100 105 110
Leu Trp Leu Ser Tyr Val Leu Ser Glu Ala Gly Arg Leu Trp Lys Asn
115 120 125
Arg Arg Tyr Asp Ala Leu Gly Arg Val Leu Ala Arg Arg Ile Ala Ala
130 135 140
Glu Glu Val Ile Glu Leu Pro Gly Leu Gly Thr Thr Leu Leu Pro Gly
145 150 155 160
Pro Gln Gly Phe Arg Arg Gly Glu Arg Gly Ala Lys Leu Asn Pro Ser
165 170 175
Tyr Leu Pro Pro Gln Leu Leu Arg Trp Phe Ala Arg Asn Arg Thr Glu
180 185 190
Ser Val Trp Val Pro Leu Arg Asp Ala Ser Leu Arg Leu Leu His Asp
195 200 205
Ser Ala Pro His Gly Leu Ala Pro Asp Trp Thr Val Phe Asp Ala Asp
210 215 220
Arg Gly Trp Ser Leu Ala Glu Leu Ala Asp Asp Glu Arg Ser Gly Ser
225 230 235 240
Tyr Asn Ala Ile Arg Val Tyr Leu Trp Leu Gly Leu Thr Asp Pro Gly
245 250 255
Asp Pro Ala Arg Gly Arg Leu Leu Ala Arg Tyr Ala Pro Met Ala Arg
260 265 270
Leu Ser Glu Leu Leu Gly Gly Val Pro Glu Lys Val Asp Pro Ala Arg
275 280 285
Pro Ala Leu Glu Gln Ser Ala Gly Ala Gln Ala Asn Gly Pro Val Gly
290 295 300
Phe Gln Ala Ala Met Leu Pro Phe Ala Asp Ala Leu Gly Gln Thr Ala
305 310 315 320
Leu Ser Glu Arg Leu Ala Asp Arg Val Ala Thr Gln Gly Val Gln Pro
325 330 335
Asp Ala Tyr Tyr Asp Gln Val Leu Ser Leu Phe Ala Leu Gly Phe Arg
340 345 350
Glu Arg Arg Tyr Arg Phe Ala Ala Asp Gly Ser Leu Gln Pro Gly Trp
355 360 365
Ala Ser Cys Asp Ala Pro Pro Gly Ser Ser Arg
370 375
<210> 74
<211> 406
<212> PRT
<213> Burkholderia ambifaria
<400> 74
Met Ala Trp Ala Phe Ala Arg Arg Arg Ala Thr Arg Pro Ala Arg Arg
1 5 10 15
Val Gly Thr Thr Leu Val Leu Ala Val Ala Ala Ala Cys Ala Ser Ala
20 25 30
Ala Thr Ala Gly Arg Ala Gln Ala Ala Gln Ser Gly Ala Ala Asp Ala
35 40 45
Gln Cys Gly Ala Ala Trp Pro Arg Trp Asp Ala Phe Lys Arg Asp Phe
50 55 60
Val Ser Ala Asp Gly Arg Val Ile Asp Val Gly Ser Ala Asp Ser Arg
65 70 75 80
Thr Val Ser Glu Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val Ala
85 90 95
Asn Asp Arg Arg Thr Phe Asp Thr Ile Leu Ala Trp Thr Glu Asn Asn
100 105 110
Leu Ala Gln Gly Asp Leu Ser Ala Arg Leu Pro Ala Trp Leu Trp Gly
115 120 125
Arg Ala Pro Asp Gly Ala Trp Arg Val Leu Asp Ala Asn Ala Ala Ser
130 135 140
Asp Ala Asp Leu Trp Ile Ala Tyr Thr Leu Val Glu Ala Gly Arg Leu
145 150 155 160
Trp Arg Glu Arg Ser Tyr Thr Ala Arg Gly Ala Leu Leu Ala Lys Arg
165 170 175
Val Leu Asp Asp Glu Thr Ala Ser Val Pro Gly Leu Gly Leu Thr Leu
180 185 190
Leu Pro Gly Pro Thr Gly Phe Lys Leu Ala Asn Gly Gln Trp Arg Val
195 200 205
Asn Pro Ser Tyr Ser Pro Pro Gln Leu Ile Arg Gly Leu Ala Ala Arg
210 215 220
Leu Pro Asp Asp Arg Arg Trp Ala Ala Leu Ser Ala Ser Thr Gly Arg
225 230 235 240
Val Leu Leu Asp Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Ala Leu
245 250 255
Tyr Arg Ala Gly Ala Gly Phe Gly Pro Asp Pro Gln Thr Arg Ala Glu
260 265 270
Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Leu Asp
275 280 285
Arg Ala Asp Pro Leu Ala Ala Pro Leu Leu Ala Arg Phe Ala Pro Phe
290 295 300
Ala Asp Tyr Ile Ala Ala His Gly Ala Pro Pro Glu Lys Val Asp Thr
305 310 315 320
Thr Thr Gly Val Ala Gly Pro Asn Asp Gly Asn Gly Gly Phe Ser Ala
325 330 335
Ala Ala Val Pro Phe Leu Asp Ala Arg Gly Gln Arg Ala Leu Ala Asp
340 345 350
Ala Gln Ala Ala Arg Val Asp Thr Leu Ala Arg Gln Ser Ala Pro Gly
355 360 365
Tyr Tyr Thr Ser Val Leu Thr Leu Phe Gly Leu Gly Trp Arg Glu Gly
370 375 380
Arg Tyr Arg Phe Gly Ala Asp Gly Ser Leu Asp Ala Arg Trp Glu Gly
385 390 395 400
Arg Pro Cys Val Ala Arg
405
<210> 75
<211> 421
<212> PRT
<213> Methylobacterium populi
<400> 75
Met Arg Pro Pro Arg Thr Pro Cys Arg Ser Arg Leu Gly Gly Thr Leu
1 5 10 15
Arg Arg Ala Gly Trp Ala Leu Ala Leu Ala Ala Ala Ser Pro Ala Ala
20 25 30
Ala Gln Pro Thr Pro Gln Pro Ala Ala Pro Gln Pro Ala Ser Pro Gln
35 40 45
Gln Thr Ser Pro Gln Met Ala Gln Pro Ala Pro Gln Gln Thr Glu Thr
50 55 60
Ser Thr Val Pro Pro Ala Thr Ala Leu Pro Gly Leu Pro Arg Ser Glu
65 70 75 80
Thr Pro Ala Arg Thr Asp Ser Gly Pro Ser Leu Ala Asn Thr Leu Asp
85 90 95
Asp Asp Ala Ala Trp Arg Ala Tyr Arg Ala Arg Phe Ile Thr Glu Gln
100 105 110
Gly Arg Ile Val Asp Thr Ala Asn Gly Met Ile Ser His Ser Glu Gly
115 120 125
Gln Gly Tyr Gly Met Leu Ile Ala Val Ala Ala Gly Asp Arg Ala Ala
130 135 140
Phe Glu Arg Ile Trp Gly Trp Thr Arg Ala Asn Leu Met Val Arg Ser
145 150 155 160
Asp Glu Leu Leu Ala Trp Arg Trp Ala Pro Asp His Arg Pro Ala Val
165 170 175
Ala Asp Met Asn Asn Ala Thr Asp Gly Asp Ile Leu Val Ala Trp Ala
180 185 190
Leu Thr Glu Ala Ala Glu Ala Trp Gly Glu Pro Ser Tyr Arg Thr Ala
195 200 205
Ala Arg Arg Ile Ala Val Glu Phe Gly Arg Lys Thr Ile Leu Phe Lys
210 215 220
Asp Pro His Gly Pro Leu Leu Leu Pro Ala Val Ser Gly Phe Ser Ala
225 230 235 240
Arg Glu Arg Ala Asp Gly Pro Leu Ile Asn Leu Ser Tyr Trp Val Phe
245 250 255
Pro Ala Phe Gln Arg Leu Pro Ile Val Ala Pro Glu Tyr Asp Trp Ala
260 265 270
Ser Leu Ile Arg Ser Gly Leu Asp Phe Leu Arg Gln Ser Arg Phe Gly
275 280 285
Pro Ser Ser Leu Pro Thr Glu Trp Ile Ser Ala Lys Asp Ser Leu Arg
290 295 300
Pro Ala Asp Gly Phe Pro Pro Leu Phe Ser Tyr Asn Ala Ile Arg Val
305 310 315 320
Pro Leu Tyr Leu Ala Trp Ala Gly Val Gly Arg Pro Glu Asp Tyr Thr
325 330 335
Pro Phe Lys Thr Leu Trp Gly Gly Ile Glu Arg Glu Arg Leu Pro Ile
340 345 350
Val Asp Thr Arg Asp Gly Gln Pro Val Glu Trp Leu Ser Glu Val Gly
355 360 365
Tyr Thr Ala Ile Pro Ala Leu Thr Ala Cys Ala Ala Asp Gly Thr Pro
370 375 380
Phe Pro Glu Ser Leu Arg Thr Val Gln Glu Asn Gln Asn Tyr Tyr Pro
385 390 395 400
Met Thr Leu His Leu Leu Ala Leu Ile Ala Ala Arg Met Arg Tyr Pro
405 410 415
Ser Cys Val Lys Ser
420
<210> 76
<211> 412
<212> PRT
<213> Opitutus terrae
<400> 76
Met Arg Phe Pro Phe Leu Pro Leu Val Leu Gly Leu Ala Leu Gly Ser
1 5 10 15
Leu Ser Ala Val Ala Ala Thr Ala Ala Gln Gly Ala Ala Glu Thr Gly
20 25 30
Arg Tyr Arg Asn Pro Phe Lys Glu Leu Leu Gly Lys Ser Asp Ala Glu
35 40 45
Ile Asn Ala Lys Leu Gln Ala Ala Trp Gln Gln Leu Phe Tyr Gly Asp
50 55 60
Glu Asn Ser Gln Arg Leu Phe Tyr Pro Ile Ala Gly Asp Met Ala Tyr
65 70 75 80
Val Pro Asp Ile Asn Asn Asn Asp Val Arg Ser Glu Gly Leu Ser Tyr
85 90 95
Gly Met Met Ile Ala Val Gln Met Asp Arg Gln Lys Glu Phe Asn Gln
100 105 110
Ile Trp Lys Phe Ala Lys His Tyr Met Tyr Tyr Asp Ala Gly Pro Phe
115 120 125
Arg Gly Tyr Phe Ala Trp His Thr Ala Phe Asp Gly Arg Arg Leu Ser
130 135 140
Pro Gly Pro Ala Pro Asp Gly Glu Glu Trp Trp Val Met Ala Leu Phe
145 150 155 160
Phe Ala Ser His Arg Trp Gly Asp Gly Glu Gly Ile Phe Asn Tyr Arg
165 170 175
Lys Glu Ala Gln Asp Leu Leu Arg Thr Met Leu His Lys Asn Glu Glu
180 185 190
Pro Asp Arg Gly Pro Ile Thr Ala Met Phe Asp Pro Val His Lys Gln
195 200 205
Ile Val Phe Val Pro Gln Gly Pro Gly Ala Gln Phe Thr Asp Pro Ser
210 215 220
Tyr His Leu Pro Ala Tyr Tyr Glu Leu Trp Ala Arg Trp Ala Ala Asp
225 230 235 240
Pro Ala Asp Arg Ala Phe Met Ala Glu Ala Ala Lys Ile Ser Arg Glu
245 250 255
His Phe Arg Thr Ala Ala His Pro Lys Thr Gly Leu Met Pro Asp Tyr
260 265 270
Ser Asn Phe Asp Gly Thr Pro Tyr Thr Ala Arg Trp Gly Asn His Gln
275 280 285
Asp Phe Leu Tyr Asp Ala Trp Arg Thr Leu Asn Asn Pro Ala Leu Asp
290 295 300
Tyr Ser Trp Trp Ala Ala Asp Pro Trp Val Val Glu Gln Ser Asn Arg
305 310 315 320
Val Leu Thr Phe Leu Ser Ser Phe Gly Ser Glu Val Pro Asp Arg Phe
325 330 335
Lys Leu Asp Gly Thr Pro Val Ser Thr Asp Thr Asn Thr Ala Gly Leu
340 345 350
Thr Ala Met Ala Ala Cys Ala Gly Leu Ala Ala Asp Ser Val Ile Ala
355 360 365
Lys Pro Trp Val Gln Gln Leu Trp Asp Met Pro Ile Pro Thr Gly Arg
370 375 380
His Arg Tyr Tyr Gly Gly Leu Leu Thr Met Ile Ala Leu Leu Glu Cys
385 390 395 400
Ser Gly Asn Phe Lys Ile Tyr Gly Pro Val Ala Lys
405 410
<210> 77
<211> 445
<212> PRT
<213> Opitutus terrae
<400> 77
Met Thr Phe Val Leu Arg Lys Pro Ala Asn Asp Cys Cys Gly Ala Arg
1 5 10 15
His Arg Ala Asp Val Ala Gly Val Gly Asp Pro Gly Pro Gly Ser Ala
20 25 30
Ser Pro Ala Thr Asn Val Ser Pro Pro Arg Phe Val Ala Trp Ala Ala
35 40 45
Ala Phe Ala Ala Leu Leu Val Ser Ser Leu Ala Ala Ala Pro Ala Pro
50 55 60
Arg Asn Leu Phe Ala Glu Leu Leu Gly Arg Ser Asp Ala Glu Ile Thr
65 70 75 80
Ala Lys Ile Asp Ala Gly Trp Gln Gln Leu Phe Tyr Gly Ser Asp Thr
85 90 95
Glu Glu Arg Leu Tyr Tyr Pro Val Asp Pro Asp Leu Ala Tyr Leu Ala
100 105 110
Asp Ile Asn Asn Gly Asp Val Arg Ser Glu Gly Met Ser Tyr Gly Met
115 120 125
Met Leu Ala Val Gln Ile Asp Arg Arg Ala Glu Phe Asp Arg Leu Tyr
130 135 140
Arg Trp Ala Arg Lys Tyr Met Leu His Thr Asp Ser Pro Arg Arg Gly
145 150 155 160
Tyr Phe Ala Trp Gln Val Ser Phe Asp Gly Arg Gln Ile Asp Pro Gly
165 170 175
Ser Ala Ser Asp Gly Glu Glu Trp Ile Ala Thr Ala Leu Leu Leu Ala
180 185 190
Ala Glu Arg Trp Gly Ser Ser Asp Ala Gly Ser Phe Asn Tyr Leu Ala
195 200 205
Asp Ala Gln Ala Leu Leu His Glu Met Leu His Lys Pro Thr Glu Gly
210 215 220
Asn Ile Thr Ala Ile Phe Ser Arg Glu Glu Lys Gln Val Val Phe Ala
225 230 235 240
Pro Thr Leu Ala Gly Ser Gln Phe Thr Asp Pro Ser Tyr His Leu Pro
245 250 255
Ala Phe Tyr Glu Val Trp Ala Arg His Ala Asp Ser Pro Glu Asp Arg
260 265 270
Ala Phe Trp Arg Asp Ala Thr Gln Thr Ser Arg Ala Phe Phe His Arg
275 280 285
Ala Ala His Pro Gln Thr Gly Leu Met Pro Glu Tyr Ala His Phe Asp
290 295 300
Gly Arg Pro Tyr Thr Gly Phe Gly Ala Glu Arg Gly Asp Phe Arg Tyr
305 310 315 320
Asp Ala Trp Arg Ile Leu Ala Asn Val Ala Phe Asp His Ala His Phe
325 330 335
Ala Ala Asp Pro Trp Gln Val Glu Gln Ser Asn Arg Ile Leu Arg Phe
340 345 350
Leu Ser Ser His Gln Pro Ala Ile Pro Asn Gln Phe Thr Leu Asp Gly
355 360 365
Arg Pro Leu Ser Thr Asp Val Asn Ser Thr Gly Leu Met Ala Met Ala
370 375 380
Ala Val Ala Gly Leu Ala Ala Glu Pro Glu Leu Ala Arg Pro Phe Val
385 390 395 400
Gln Phe Leu Trp Asp Ala Pro Ile Pro Arg Gly Gln Tyr Arg Tyr Tyr
405 410 415
Asp Gly Val Leu Tyr Gln Leu Ala Leu Leu Gln Val Ser Gly Arg Phe
420 425 430
Gln Leu Ser Phe His Leu Thr Ala Pro Thr Thr Ser Lys
435 440 445
<210> 78
<211> 393
<212> PRT
<213> Cupriavidus taiwanensis
<400> 78
Met Thr Asp Gln Thr Arg Arg Arg Tyr Val Thr Tyr Ala Cys Ala Leu
1 5 10 15
Gly Ala Ser Ala Leu Thr Ala Ala Trp Pro Thr Glu Thr Arg Ala His
20 25 30
Ala Pro Pro Ala Thr Arg Arg Ala Ser Arg Trp Pro Leu Tyr Arg Gln
35 40 45
Phe Leu Glu Arg Phe Val Gln Ala Asp Gly Arg Val Ile Asp Tyr Ser
50 55 60
Thr Pro Thr Leu Gln Ser Thr Ser Glu Gly Gln Ser Tyr Gly Met Val
65 70 75 80
Phe Ala Leu Val Ala Asp Asp Arg Asp Ala Phe Asp Arg Leu Trp Arg
85 90 95
Trp Ser Val Ala His Leu Gly Asn Gly Arg Leu Asp Asp Lys Leu Pro
100 105 110
Ala Trp Gln Trp Gly Arg Arg Ala Asp Gly Thr Trp Gly Val Leu Asp
115 120 125
Arg Asn Pro Ala Ala Asp Ala Asp Leu Trp Phe Ala Phe Ala Leu Ala
130 135 140
Glu Ala Ala Arg Leu Trp Gln Ala Pro Ser Tyr Ala Asp Ala Ala Arg
145 150 155 160
Ala Leu Leu Arg Arg Val Ala Ala Gln Glu Val Val Thr Leu Pro Gly
165 170 175
Phe Gly Pro Met Leu Leu Pro Gly Pro Gln Gly Phe Ile Asp Asp Gly
180 185 190
Pro Asp Gly Ser Arg Arg Trp Arg Leu Asn Ala Ser Tyr Leu Pro Val
195 200 205
Pro Leu Leu Arg Arg Leu Ala Ala Phe Asp Pro Ser Gly Pro Trp Asp
210 215 220
Lys Leu Ala Ala Gln Val Ala Arg Leu Ile Gly Ala Val Ser His Ala
225 230 235 240
Gly Ile Val Pro Asp Trp Ser Ala Tyr Arg Val Gly Gly Arg Arg Gln
245 250 255
Gly Phe Val Arg Asp Pro Val Lys Gly Asp Val Ser Ser Tyr Asp Ala
260 265 270
Val Arg Val Tyr Leu Trp Ala Gly Met Thr Pro Asp Gln Asp Ser Thr
275 280 285
Trp Gly Ala Ile Met Gln Ala Leu Leu Pro Asn Ala Ala Arg Leu Ala
290 295 300
Gly Arg Ala Ala Pro Pro Glu Lys Met Tyr Ala Glu Ser Gly Lys Val
305 310 315 320
Glu Gly Ile Gly Pro Pro Ala Phe Ser Ala Ala Leu Leu Pro Phe Leu
325 330 335
Gln Ala Ala Gly Ser Pro Ala Leu Ala Thr Gln Met Ala Arg Ala Gln
340 345 350
Lys Ile Val Gly Ala Pro Pro Gly Pro Gly Gly Pro Thr Tyr Tyr Asp
355 360 365
Thr Val Leu Gly Leu Phe Gly Leu Gly Phe Met Glu Gly Arg Tyr Arg
370 375 380
Phe Ser Ala Ser Gly Gln Leu Asp Arg
385 390
<210> 79
<211> 388
<212> PRT
<213> Burkholderia phymatum
<400> 79
Met Arg Arg Lys Arg Leu Leu Thr Gln Arg Phe Ala Arg Ile Pro Lys
1 5 10 15
Cys Gly Ala Val Arg Arg Leu Val Gly Leu Ala Val Ala Cys Leu Gln
20 25 30
Val Ser Val Ala Ala His Ala Asp Cys Arg Trp Pro Leu Trp Glu Arg
35 40 45
Tyr Ala Gln Gln Gln Ile Ser Lys Asp Gly Arg Ile Val Glu Pro Gly
50 55 60
Lys Gly Asp Arg Thr Thr Ser Glu Ala Gln Ala Tyr Ala Met Phe Phe
65 70 75 80
Ala Val Val Gly Asp Asp Arg Val Arg Phe Asp Ser Leu Leu Asn Trp
85 90 95
Ala Leu Ala His Thr Trp Ala Thr Thr Gly His Pro Pro Ala Trp Leu
100 105 110
Trp Ala Lys Arg Ala Asp Gly Thr Phe Asp Val Leu Asp Asp Asn Ser
115 120 125
Ala Thr Asp Ala Asp Leu Trp Met Ala Tyr Ala Met Leu Glu Ala Ala
130 135 140
Arg Arg Trp Ser Glu Pro Arg Tyr Arg Ala Leu Gly Leu Arg Leu Leu
145 150 155 160
Asp Gln Ile Thr Ala Thr Glu Val Val Trp Gln Asp Glu Thr Ser Ala
165 170 175
Phe Leu Ile Pro Gly Arg Leu Gly Phe Arg Leu Ser Glu His Ala Tyr
180 185 190
Leu Val Asn Pro Ser Tyr Ala Pro Ile Gln Leu Leu Arg Arg Phe Ile
195 200 205
Lys Glu Gln Pro His Gly Pro Trp Val Ser Val Leu Asn His Gln Val
210 215 220
Asp Leu Leu Val Ala Ser Ala Pro Gln Gly Phe Val Pro Asp Trp Tyr
225 230 235 240
Arg Phe Asp Thr Phe Lys Gly Tyr Met Leu Glu Pro Ser Lys Gly Pro
245 250 255
Thr Gly Gly Phe Asp Ala Val Arg Thr Tyr Met Trp Ala Gly Met Leu
260 265 270
Asp Pro Thr Asp Pro Ala Arg Gly Glu Val Ile Pro Ala Leu Ser Gly
275 280 285
Met Ala Asp Leu Val Ala Gln Thr Gly Arg Met Pro Leu Tyr Val Asn
290 295 300
Ile Glu Ser Gly Glu Thr Phe Asp Glu Gly Ser Ala Gly Phe Arg Ala
305 310 315 320
Ala Val Ile Pro Leu Leu Phe Val Thr Gly His Pro Ser Ala Ala Ser
325 330 335
Arg Leu Ala Gln Glu Thr Lys Val Met Gln Gln Ala Arg Glu Trp Asp
340 345 350
Gly Leu His Tyr Tyr Asp Arg Asn Leu Leu Leu Phe Ala Ala Gly Trp
355 360 365
Leu Glu Gly Arg Tyr Arg Phe Asp His Glu Gly Thr Leu Gln Leu Lys
370 375 380
Pro Cys Arg Pro
385
<210> 80
<211> 410
<212> PRT
<213> Burkholderia phymatum
<400> 80
Met Arg Glu Ala Phe Ile Phe Ser Ser Trp Arg Phe Arg Leu Arg Ala
1 5 10 15
Pro Leu Ala Arg Pro Leu Val Cys Ala Gly Leu Val Gly Ser Leu Val
20 25 30
Ala Ser Met Thr Gly His Ala Gln Ala Ala Thr Cys Asp Asp Trp Pro
35 40 45
Ala Tyr Arg Ala Phe Val Ser Arg Phe Val Gln Gln Asp Gly Arg Val
50 55 60
Val Asp Phe Ser Thr Pro Thr Gln Gln Thr Thr Ser Glu Gly Gln Ser
65 70 75 80
Tyr Gly Met Phe Phe Ala Leu Val Ala Asn Asp Arg Ala Thr Phe Glu
85 90 95
Arg Leu Leu Arg Trp Thr Arg Ala Asn Leu Ser Ala Gly Arg Phe Asp
100 105 110
Gly Gly Asp Val Lys Leu Pro Ala Trp Gln Trp Gly Lys Lys Pro Asp
115 120 125
Gly Ala Tyr Gly Val Ile Asp Pro Asn Ser Ala Ala Asp Ser Asp Leu
130 135 140
Trp Ile Ala Tyr Asp Leu Phe Glu Ala Gly Arg Leu Trp Arg Glu Pro
145 150 155 160
Ala Tyr Thr Gln Leu Ala Tyr Ala Leu Val Thr Gln Val Glu Lys Gln
165 170 175
Glu Val Ala Asp Leu Arg Gly Leu Gly Pro Met Leu Leu Pro Gly Ser
180 185 190
Gln Gly Phe Arg Asn Gly Ala Thr Thr Arg Leu Asn Pro Ser Tyr Leu
195 200 205
Pro Leu Pro Leu Ile Arg Ala Leu Ala Ala Gln Ser Pro Asn Gly Pro
210 215 220
Trp Ser Arg Ile Ala Asp Asn Ala Phe Lys Leu Val Lys Thr Thr Ala
225 230 235 240
Pro Leu Gly Phe Ala Pro Asp Trp Ala Ala Tyr Arg Asp Gly Gln Phe
245 250 255
Val Val Asp Pro Gln Thr Gly Asp Ala Gly Ser Tyr Asp Ala Ile Arg
260 265 270
Val Tyr Leu Trp Ala Gly Leu Ala Ala Ser Ala Asp Pro Leu Ala Lys
275 280 285
Pro Trp Leu Ala Ala Leu His Gly Met Arg Asp Arg Ile Ala Gln Thr
290 295 300
Gly Val Pro Pro Glu Lys Val Ala Thr Thr Thr Gly Asn Ala Ala Gly
305 310 315 320
Glu Gly Pro Ile Gly Phe Trp Gly Ala Leu Leu Pro Tyr Phe Arg Ala
325 330 335
Leu Gly Asp Thr Arg Ala Ala Gly Leu Ala Gln Thr His Leu Ala Ser
340 345 350
Leu Ala Thr Pro Ala Pro Gly Ala Ala Gln Ala Ala Arg Asn Gln Pro
355 360 365
Val Tyr Tyr Asp Glu Val Leu Met Leu Phe Gly Thr Ala Ser Ala Asp
370 375 380
Gly Arg Tyr Arg Phe Asp Glu Asn Gly Arg Leu Val Pro Arg Trp Glu
385 390 395 400
Asn Thr Cys Gln Ser Ala Asn Ala Arg Leu
405 410
<210> 81
<211> 365
<212> PRT
<213> 大肠杆菌
<400> 81
Met Arg Ser Gly Ile Val Thr Met Leu Leu Leu Ala Ala Phe Ser Val
1 5 10 15
Gln Ala Ala Cys Thr Trp Pro Ala Trp Glu Gln Phe Lys Lys Asp Tyr
20 25 30
Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser Asp Ala Arg Lys Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe Phe Ala Leu Ala Ala
50 55 60
Asn Asp Arg Ala Ala Phe Asp Asn Ile Leu Asp Trp Thr Gln Asn Asn
65 70 75 80
Leu Ala Gln Gly Ser Leu Lys Glu Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Lys Glu Asn Ser Lys Trp Glu Val Leu Asp Ser Asn Ser Ala Ser
100 105 110
Asp Gly Asp Val Trp Met Ala Trp Ser Leu Leu Glu Ala Gly Arg Leu
115 120 125
Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser Ala Leu Leu Lys Arg
130 135 140
Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly Leu Gly Ser Met Leu
145 150 155 160
Leu Pro Gly Lys Val Gly Phe Ala Glu Asp Asn Ser Trp Arg Phe Asn
165 170 175
Pro Ser Tyr Leu Pro Pro Thr Leu Ala Gln Tyr Phe Thr Arg Phe Gly
180 185 190
Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln Arg Leu Leu Leu Glu
195 200 205
Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg Tyr Glu Lys Asp
210 215 220
Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile Ser Ser Tyr Asp
225 230 235 240
Ala Ile Arg Val Tyr Met Trp Val Gly Met Met Pro Asp Ser Asp Pro
245 250 255
Gln Lys Ala Arg Met Leu Asn Arg Phe Lys Pro Met Ala Thr Phe Thr
260 265 270
Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp Val Ala Thr Gly Lys
275 280 285
Ala Gln Gly Lys Gly Pro Val Gly Phe Ser Ala Ala Met Leu Pro Phe
290 295 300
Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln Arg Val Ala Asp
305 310 315 320
Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val Leu Thr Leu Phe
325 330 335
Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Ser Thr Lys Gly Glu
340 345 350
Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn Ser His
355 360 365
<210> 82
<211> 393
<212> PRT
<213> Burkholderia phytofirmans
<400> 82
Met Arg Val Arg Ile Asn Lys Lys Ile Gly Val Ala Leu Ala Leu Gly
1 5 10 15
Leu Ala Ala Ser Val Ser Phe Ala Asn Leu Val Lys Val Ala Gln Thr
20 25 30
Thr Ala Ala Pro Gly Ala Cys Gly Asp Trp Ser Gly Tyr Arg Ala Phe
35 40 45
Val Glu Arg Phe Val Gln Ala Asp Gly Arg Val Ile Asp Tyr Ser Thr
50 55 60
Pro Ala Gln Gln Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe
65 70 75 80
Ala Leu Val Ala Asn Asp Arg Ala Thr Phe Asp Arg Leu Leu Gly Trp
85 90 95
Thr Arg Thr Asn Leu Ala Gly Asn Gln Phe Asp Ala Gln Asn Met Arg
100 105 110
Leu Pro Ser Trp Gln Trp Gly Arg Lys Ala Asp Gly Ser Tyr Gly Val
115 120 125
Leu Asp Pro Asn Ser Ala Ser Asp Ser Asp Leu Trp Ile Ala Tyr Asp
130 135 140
Leu Leu Gln Ala Gly Arg Leu Trp His Glu Ala Ala Tyr Thr Gln Leu
145 150 155 160
Gly Glu Ala Leu Ala Ala Gln Ile Ala Arg Gln Glu Met Thr Thr Leu
165 170 175
Pro Gly Val Gly Pro Met Leu Leu Pro Gly Pro Gln Gly Phe Lys Ser
180 185 190
Gly Gly Val Thr Arg Leu Asn Pro Ser Tyr Leu Pro Leu Pro Val Leu
195 200 205
Arg Ser Leu Ala His Asp Met Pro Asn Gly Pro Trp Gly Lys Leu Ala
210 215 220
Asp Ser Ala Tyr Lys Leu Ile Lys Thr Thr Ala Pro Gln Gly Phe Ala
225 230 235 240
Pro Asp Trp Ala Ala Trp Gln Asn Gly Gln Phe Val Val Asp Pro Lys
245 250 255
Asn Gly Asp Thr Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala
260 265 270
Gly Leu Ala Ser Pro Ala Asp Pro Leu Ala Lys Pro Trp Leu Ala Ala
275 280 285
Leu Gly Gly Met Arg Ala Arg Val Ala Gln Thr Gly Phe Pro Pro Glu
290 295 300
Lys Val Ser Ser Thr Ser Gly Thr Ala Ser Gly Glu Gly Pro Leu Ser
305 310 315 320
Tyr Trp Ala Ala Leu Ala Pro Tyr Phe Lys Ala Leu Gly Asp Glu Arg
325 330 335
Gly Leu Gly Leu Ala Arg Thr His Leu Ala Ala Leu Asp Thr Asn Val
340 345 350
Pro Gly Arg Glu Pro Val Tyr Tyr Asp Arg Val Leu Gly Leu Phe Gly
355 360 365
Thr Gly Phe Ile Asp Gly Arg Tyr Arg Phe Asp Glu Ala Gly Arg Leu
370 375 380
Val Pro Ser Trp Arg Lys Glu Cys Asp
385 390
<210> 83
<211> 365
<212> PRT
<213> 鲍氏志贺氏菌
<400> 83
Met Arg Ser Gly Ile Val Thr Met Leu Leu Leu Ala Ala Phe Ser Val
1 5 10 15
Gln Ala Ala Cys Thr Trp Pro Ala Trp Glu Gln Phe Lys Lys Asp Tyr
20 25 30
Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser Asp Ala Arg Lys Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe Phe Ala Leu Ala Ala
50 55 60
Asn Asp Arg Ala Ala Phe Asp Asn Ile Leu Asp Trp Thr Gln Asn Asn
65 70 75 80
Leu Ala Gln Gly Ser Leu Lys Glu Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Lys Glu Asn Ser Lys Trp Glu Val Leu Asp Ser Asn Ser Ala Ser
100 105 110
Asp Gly Asp Val Trp Met Ala Trp Ser Leu Leu Glu Ala Gly Arg Leu
115 120 125
Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser Ala Leu Leu Lys Arg
130 135 140
Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly Leu Gly Ser Met Leu
145 150 155 160
Leu Pro Gly Lys Val Gly Phe Ala Glu Asp Asn Ser Trp Arg Phe Asn
165 170 175
Pro Ser Tyr Leu Pro Pro Thr Leu Ala Gln Tyr Phe Thr Arg Phe Gly
180 185 190
Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln Arg Leu Leu Leu Glu
195 200 205
Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg Tyr Glu Lys Asp
210 215 220
Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile Ser Ser Tyr Asp
225 230 235 240
Ala Ile Arg Val Tyr Met Trp Val Gly Met Met Pro Asp Ser Asp Pro
245 250 255
Gln Lys Ala Arg Met Leu Asn Arg Phe Lys Pro Met Ala Thr Phe Thr
260 265 270
Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp Val Ala Thr Gly Lys
275 280 285
Ala Gln Gly Lys Gly Pro Val Gly Phe Ser Ala Ala Met Leu Pro Phe
290 295 300
Leu Gln Asn Arg Asp Ala Gln Val Val Gln Arg Gln Arg Val Ala Asp
305 310 315 320
Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val Leu Thr Leu Phe
325 330 335
Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Ser Thr Lys Gly Glu
340 345 350
Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn Ser His
355 360 365
<210> 84
<211> 333
<212> PRT
<213> Erwinia tasmaniensis
<400> 84
Met Met Ala Met Phe Arg Thr Gly Leu Leu Leu Leu Ala Leu Met Leu
1 5 10 15
Gly Met Ala Gln Ala Ala Ala Ala Asp Gly Trp Ser Ser Phe Lys Leu
20 25 30
Arg Phe Met Thr Ser Asp Gly Arg Ile Gln Asp Thr Gly Asn Gln Asn
35 40 45
Val Ser His Thr Glu Gly Gln Gly Tyr Ala Met Leu Met Ala Val Tyr
50 55 60
Tyr Asp Asp Arg Ser Ser Phe Asp Lys Leu Trp Arg Trp Thr Gln Asn
65 70 75 80
Asn Leu Ser Asn Pro Lys Asn Gly Leu Phe Tyr Trp Lys Tyr Asn Pro
85 90 95
Ala Ala Arg Asp Pro Val Ser Asp Lys Asn Asn Ala Ala Asp Gly Asp
100 105 110
Val Leu Ile Ala Trp Ala Leu Leu Gln Ala Gly Glu Lys Trp Gln Val
115 120 125
Ala Gly Trp Leu Gln Gln Ser Asp Arg Ile Gln Arg Ala Ile Val Ala
130 135 140
His Asn Val Ile Val Phe Gly Gly Arg Thr Leu Met Leu Pro Gly Ala
145 150 155 160
Glu Gly Phe Asn Lys Thr Ser Tyr Val Val Leu Asn Pro Ser Tyr Phe
165 170 175
Val Phe Pro Ala Trp Arg Asp Phe Ala Arg Arg Ser His Leu Lys Leu
180 185 190
Trp Asp Lys Leu Ile Lys Asp Gly Leu Asp Leu Leu Thr Glu Met Arg
195 200 205
Phe Gly Asp Thr Ala Leu Pro Leu Asp Trp Val Ala Met Asn Val Asp
210 215 220
Gly Ser Leu Ala Pro Ala Thr Ala Trp Pro Ala Arg Phe Ser Tyr Asp
225 230 235 240
Ala Ile Arg Ile Pro Leu Tyr Leu His Trp Tyr Asp Ala Ala Ser Leu
245 250 255
Arg Leu Val Pro Phe Gln Arg Phe Trp Met Gly Phe Pro Arg Leu Gln
260 265 270
Thr Pro Ala Trp Ile Asp Val Leu Thr Asn Glu Lys Ala Pro Tyr Asn
275 280 285
Met Ala Gly Gly Leu Leu Ala Val Arg Asp Leu Thr Leu Asp Asp Ala
290 295 300
Gly Tyr Leu Asn Asp Ser Pro Gly Ala Ser Glu Asp Tyr Tyr Ser Ser
305 310 315 320
Ser Leu Gln Leu Leu Ala Trp Leu Ala Phe Lys Ser Arg
325 330
<210> 85
<211> 419
<212> PRT
<213> Bacteroides intestinalis
<400> 85
Met Lys Asn Leu Phe Tyr Leu Leu Leu Cys Leu Ile Ala Gly Thr Ser
1 5 10 15
Cys Ser Gln Ala Asp Pro Thr Lys Pro Trp Asp Lys Gly Ala Phe Glu
20 25 30
Thr Gln Lys Tyr Arg Asn Leu Leu Ala Glu Met Gly Tyr Lys Gln Ala
35 40 45
Asp Ile Asp Ala Lys Leu Lys Ser Val Phe Asp Gly Val Phe Tyr Gly
50 55 60
Pro Asp Lys Val Tyr Phe Glu Val Gly Asp Ser Met Ala Tyr Ile Ser
65 70 75 80
Asp Ile Lys Asn His Asp Val Arg Thr Glu Gly Met Ser Tyr Gly Leu
85 90 95
Met Ile Ala Val Gln Phe Asp Arg Lys Asp Ile Phe Asp Arg Leu Trp
100 105 110
Arg Trp Gly Thr Lys Tyr Met Gln His Gln Asp Gly Pro Leu Lys Gly
115 120 125
Tyr Phe Ala Trp Ser Cys Glu Thr Asp Gly Thr Arg Asn Ser Gln Gly
130 135 140
Pro Ala Ser Asp Gly Glu Leu Tyr Tyr Val Thr Ala Leu Ile Phe Ala
145 150 155 160
Ser Asn Arg Trp Gly Asn Asp Thr Gly Ile Asn Tyr Leu Ala Glu Ala
165 170 175
Arg Asn Ile Leu Asn Cys Ser Met Glu Lys Asp Gly Thr Asp Arg Val
180 185 190
Met Pro Phe Ile Asn Val Glu His Lys Leu Ile Thr Phe Val Pro Asp
195 200 205
Ile Arg Gly Gly Leu Phe Thr Asp Pro Ser Tyr His Val Pro Ala Phe
210 215 220
Tyr Glu Val Trp Ala Arg Trp Ala Asp Asp Gly Arg Ala Asp Phe Trp
225 230 235 240
Arg Glu Cys Ala Glu Cys Ser Arg Glu Tyr Leu His Lys Ser Ile His
245 250 255
Pro Val Thr Gly Leu Asn Pro Asp Tyr Asn Asn Tyr Asp Gly Ser Leu
260 265 270
Leu Gly Asn Asn Arg Ile Ile Gly Asp Ala Phe Arg Phe Asp Ser Trp
275 280 285
Arg Val Pro Met Asn Ile Ala Leu Asp Tyr Ser Trp Ala Cys Ala Asp
290 295 300
Lys Glu Trp Gln Gln Glu Tyr Gly Asn Lys Ile Gln Asn Phe Leu Tyr
305 310 315 320
Ser Gln Gly Ile Asp Thr Phe Val Asp Gln Tyr Asn Ile Asp Gly Thr
325 330 335
Gln Val Lys Asp Thr Leu Arg Ala Gly Glu His Lys Ala Leu Arg His
340 345 350
Ser Leu Gly Leu Val Ala Thr Ser Ala Val Ala Ser Leu Met Cys Thr
355 360 365
His Glu Lys Ser Arg Glu Phe Val Asp Lys Leu Trp Asn Ala Lys His
370 375 380
Glu Pro Tyr Glu Asp Gly Tyr Phe Asp Ala Tyr Tyr Asp Gly Leu Leu
385 390 395 400
Arg Leu Phe Ala Phe Met His Leu Ser Gly Asn Tyr Arg Ile Ile Phe
405 410 415
Pro Glu Lys
<210> 86
<211> 418
<212> PRT
<213> Bacteroides intestinalis
<400> 86
Met Met Lys Leu Thr Thr Leu Phe Ala Val Ser Val Ser Leu Ile Leu
1 5 10 15
Ser Gly Phe Cys Ser Leu Gly Val Gln Ala His Pro Val Gln Glu Asp
20 25 30
Ser Ser Gly Gly Pro Val Pro Ala Gly Ala Tyr Tyr Thr Asp Asn Tyr
35 40 45
Arg Asn Leu Phe Asn Glu Tyr Leu Gly Ile Ser Gln Gln Gln Thr Asp
50 55 60
Gln Lys Met Glu Gln Ile Trp Asn His Phe Phe Val Asn Glu Lys Thr
65 70 75 80
Lys Val Tyr Tyr Glu Ser Asp Asp Asn Thr Ala Tyr Ile Tyr Asp Thr
85 90 95
Gly Asn Gln Asp Val Arg Thr Glu Gly Met Ser Tyr Gly Met Met Ile
100 105 110
Cys Val Gln Leu Asp Lys Gln Ala Glu Phe Asp Lys Leu Trp Arg Trp
115 120 125
Ala Lys Lys Tyr Met Leu Tyr Thr Ser Gly Lys Trp Ser Gly Tyr Tyr
130 135 140
Ala Trp His Cys Thr Pro Arg Gly Val Lys Ile Gly Lys Glu Pro Ser
145 150 155 160
Cys Ala Ser Asp Gly Glu Ile Tyr Phe Ile Thr Ser Leu Phe Phe Ala
165 170 175
Ser His Arg Trp Gly Asn Asp Gly Ala Tyr Asp Tyr Asn Gln Glu Ala
180 185 190
Gln Lys Ile Leu Lys Asp Val Met Ser Lys Asp Gly Ser Gln Gly Val
195 200 205
Tyr Asn Leu Phe Asn Thr Glu Ser Lys Leu Val Thr Phe Val Pro Glu
210 215 220
Lys Val Tyr Tyr Asn Tyr Thr Asp Pro Ser Tyr Asn Leu Pro Ala Phe
225 230 235 240
Phe Glu Leu Trp Ala Leu Trp Ser Asp Thr Asn Lys Glu Phe Trp Lys
245 250 255
Gln Thr Pro Asp Ala Ala Arg Arg Leu Leu Ala Asp Ala Ser His Lys
260 265 270
Lys Thr Gly Leu Phe Pro Asp Tyr Ser Ala Phe Asp Gly Thr Pro Trp
275 280 285
Lys Pro Lys Asn Trp Gly Tyr Asp Thr Arg Arg Tyr Gln Phe Asp Ala
290 295 300
Leu Arg Cys Ala Met Asn Val Gly Met Asp Tyr Tyr Trp Phe Gly Lys
305 310 315 320
Asp Ala Thr Asn Gln Ala Glu Met Met Ser Arg Leu Leu Asn Phe Phe
325 330 335
Lys Gln Asp Asn Phe Thr His Glu Tyr Phe Asn Val Asp Gly Ser Ala
340 345 350
Pro Ala Gly Asn Tyr Ser Thr Gly Met Ile Gly Ala Asn Ala Val Gly
355 360 365
Ala Phe Ala Leu Asn Asp Lys Asn Leu Ala Lys Glu Cys Ile Gln Lys
370 375 380
Leu Trp Asn Glu Pro Leu Pro Thr Gly Lys Phe Arg Tyr Tyr Ser Gly
385 390 395 400
Met Val Tyr Met Met Ser Met Leu His Val Ser Gly Asn Phe Arg Ile
405 410 415
Ile Lys
<210> 87
<211> 407
<212> PRT
<213> 地芽孢杆菌属
<400> 87
Met Lys Val Phe Arg Asn Ser Ile Ile Arg Lys Ser Ala Ala Leu Val
1 5 10 15
Cys Ala Val Leu Leu Ile Leu Pro Ala Gly Leu Ser Leu Ala Ala Asn
20 25 30
Lys Pro Phe Pro Gln His Thr Ser Tyr Thr Ser Gly Ser Ile Lys Pro
35 40 45
Asn Asn Val Thr Gln Thr Ala Met Asp Asn Ala Val Lys Ser Lys Trp
50 55 60
Asn Ser Trp Lys Gly Ser Phe Leu Lys Pro Ala Ala Thr Gly Gln Tyr
65 70 75 80
Tyr Val Lys Tyr Asn Ser Ala Gly Glu Thr Val Ser Glu Ala His Gly
85 90 95
Tyr Gly Met Ile Phe Thr Val Leu Met Ala Gly Tyr Asp Ser Asn Ala
100 105 110
Gln Ser Tyr Phe Asp Gly Leu Tyr Arg Tyr Tyr Lys Ala His Pro Ser
115 120 125
Asp Asn Asn Pro Tyr Leu Met Ala Trp Lys Gln Asn Ser Ser Phe Gln
130 135 140
Asn Ile Glu Gly Ala Asn Ser Ala Thr Asp Gly Asp Met Asp Ile Ala
145 150 155 160
Tyr Ala Leu Leu Leu Ala Asp Lys Gln Trp Gly Ser Ser Gly Ser Ile
165 170 175
Asn Tyr Leu Gln Ala Ala Lys Asp Ile Ile Asn Ala Ile Met Ser Asn
180 185 190
Asp Val Asn Gln Ser Gln Trp Thr Leu Arg Leu Gly Asp Trp Ala Thr
195 200 205
Ser Gly Asn Tyr Asn Thr Ala Thr Arg Pro Ser Asp Phe Met Leu Asn
210 215 220
His Met Lys Ala Phe Arg Ala Ala Thr Gly Asp Ala Arg Trp Asp Asn
225 230 235 240
Val Ile Asn Lys Thr Tyr Thr Ile Ile Asn Ser Ile Tyr Asn Gly Tyr
245 250 255
Ser Ser Asn Thr Gly Leu Leu Pro Asp Phe Val Val Met Ser Gly Gly
260 265 270
Asn Tyr Gln Pro Ala Ala Ala Gly Phe Leu Glu Gly Ala Asn Asp Gly
275 280 285
Lys Tyr Tyr Tyr Asn Ser Ala Arg Thr Pro Trp Arg Ile Thr Thr Asp
290 295 300
Tyr Leu Met Thr Gly Asp Thr Arg Ala Leu Asn Gln Leu Asn Lys Met
305 310 315 320
Asn Thr Phe Ile Lys Ser Ala Ala Asn Ser Asn Pro Ala Asn Ile Lys
325 330 335
Ala Gly Tyr Asn Leu Asn Gly Thr Ala Leu Val Thr Tyr Asn Ser Gly
340 345 350
Ala Phe Tyr Ala Pro Phe Gly Val Ser Ala Met Thr Ser Ser Ser His
355 360 365
Gln Ser Trp Leu Asn Ser Val Trp Asn Tyr Thr Ala Asn Ala Ser Ala
370 375 380
Glu Gly Tyr Tyr Glu Glu Ser Ile Lys Leu Phe Ser Met Met Val Met
385 390 395 400
Ser Gly Asn Trp Trp Thr Tyr
405
<210> 88
<211> 348
<212> PRT
<213> Rhizobium etli
<400> 88
Met Arg Leu Trp Arg Ala Leu Leu Val Ala Ala Thr Ala Met Leu Ala
1 5 10 15
Pro Ala Ile Pro Pro Ala Val Ala Gln Gln Ala Met Ile Asn Ala Asp
20 25 30
Ala Trp Ser Ala Tyr Lys Ser Lys Phe Leu Asp Pro Ser Gly Arg Ile
35 40 45
Ile Asp Asn Gly Asn Gly Asn Ile Ser His Ser Glu Gly Gln Gly Tyr
50 55 60
Gly Met Leu Leu Ala Tyr Leu Ser Ala Ser Pro Ala Asp Phe Glu Gln
65 70 75 80
Ile Trp Tyr Phe Thr Arg Thr Glu Leu Leu Leu Arg Asp Asp Gly Leu
85 90 95
Ala Val Trp Lys Trp Asp Pro Asn Val Lys Pro His Val Thr Asp Thr
100 105 110
Asn Asn Ala Thr Asp Gly Asp Met Leu Ile Ala Tyr Ala Leu Ala Leu
115 120 125
Ala Gly Thr Gln Trp Asn Arg Asn Asp Tyr Ile Leu Ala Ala Ser Arg
130 135 140
Met Ala Glu Ala Leu Leu Ala Lys Thr Val Ala Arg Ser Ala Gly Gln
145 150 155 160
Met Leu Leu Leu Pro Gly Ser Glu Gly Phe Thr Ala Ala Asp Arg Lys
165 170 175
Asp Gly Pro Val Val Asn Pro Ser Tyr Trp Ile Tyr Glu Ala Ile Pro
180 185 190
Val Met Ala Ala Leu Ala Pro Ser Asp Ala Trp Lys Glu Leu Ser Asp
195 200 205
Asp Gly Val Ala Leu Leu Lys Thr Met Gln Phe Gly Pro Arg Lys Leu
210 215 220
Pro Ala Glu Trp Val Ser Leu Ser Gly Pro Pro Arg Pro Ala Glu Gly
225 230 235 240
Phe Asp Ala Glu Phe Ala Tyr Asn Ala Leu Arg Ile Pro Leu Tyr Leu
245 250 255
Ala Arg Gly Gly Ile Thr Asp Lys Ala Leu Leu Thr Arg Leu Arg Lys
260 265 270
Gly Met Ser Gln Asp Gly Ile Pro Ala Thr Ile Asp Leu Thr Thr Gly
275 280 285
Arg Pro Lys Thr Val Leu Ser Asp Pro Gly Tyr Arg Ile Val Asn Asp
290 295 300
Val Val Ala Cys Val Val Asp Gly Thr Lys Leu Pro Ser Ser Ala Leu
305 310 315 320
Gln Phe Ala Pro Thr Leu Tyr Tyr Pro Ser Thr Leu Gln Leu Leu Gly
325 330 335
Leu Ala Tyr Ile Gly Glu Lys His Pro Glu Cys Leu
340 345
<210> 89
<211> 370
<212> PRT
<213> 大肠杆菌
<400> 89
Met Lys Met Asn Val Leu Arg Ser Gly Ile Val Thr Met Leu Leu Leu
1 5 10 15
Ala Ala Phe Ser Val Gln Ala Ala Cys Thr Trp Pro Ala Trp Glu Gln
20 25 30
Phe Lys Lys Asp Tyr Ile Ser Gln Glu Gly Arg Val Ile Asp Pro Ser
35 40 45
Asp Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Gly Met Phe
50 55 60
Phe Ala Leu Ala Ala Asn Asp Arg Val Ala Phe Asp Asn Ile Leu Asp
65 70 75 80
Trp Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Lys Glu Arg Leu Pro
85 90 95
Ala Trp Leu Trp Gly Lys Lys Glu Asn Ser Lys Trp Glu Val Leu Asp
100 105 110
Ser Asn Ser Ala Ser Asp Gly Asp Val Trp Met Ala Trp Ser Leu Leu
115 120 125
Glu Ala Gly Arg Leu Trp Lys Glu Gln Arg Tyr Thr Asp Ile Gly Ser
130 135 140
Ala Leu Leu Lys Arg Ile Ala Arg Glu Glu Val Val Thr Val Pro Gly
145 150 155 160
Leu Gly Ser Met Leu Leu Pro Gly Lys Val Gly Phe Ala Glu Asp Asn
165 170 175
Ser Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Thr Leu Ala Gln Tyr
180 185 190
Phe Thr Arg Phe Gly Ala Pro Trp Thr Thr Leu Arg Glu Thr Asn Gln
195 200 205
Arg Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val
210 215 220
Arg Tyr Glu Lys Asp Lys Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu
225 230 235 240
Ile Ser Ser Tyr Asp Ala Ile Arg Val Tyr Met Trp Val Gly Met Met
245 250 255
Pro Asp Ser Asp Pro Gln Lys Ala Arg Met Leu Asn Arg Phe Lys Pro
260 265 270
Met Ala Thr Phe Thr Glu Lys Asn Gly Tyr Pro Pro Glu Lys Val Asp
275 280 285
Val Ala Thr Gly Lys Ala Gln Gly Lys Gly Pro Val Gly Phe Ser Ala
290 295 300
Ala Met Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg
305 310 315 320
Gln Arg Val Ala Asp Asn Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr
325 330 335
Val Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe
340 345 350
Ser Thr Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Ala Asn
355 360 365
Ser His
370
<210> 90
<211> 380
<212> PRT
<213> 路氏乳杆菌
<400> 90
Met Lys Arg Pro Val Tyr Ile Trp Leu Leu Thr Leu Val Val Ala Val
1 5 10 15
Ile Tyr Ile Ala Thr Leu Ala Phe Val Arg Ile Lys Asn Pro Glu His
20 25 30
Ile Gln Tyr Glu Ala Tyr Arg Arg Trp Gln Ala Ala Tyr Leu Val Arg
35 40 45
Lys Asn Ser Lys Gln Thr Tyr Val Asn Thr Ser Asn Asp Gln Lys His
50 55 60
Pro Val Ala Leu Ser Glu Gly Gln Gly Tyr Gly Leu Gln Val Val Ser
65 70 75 80
Arg Ala Ala Glu Lys Gly Trp Ala Ser Glu Asn Asp Phe Asp Lys Leu
85 90 95
Leu Asn Tyr Tyr Leu Ala His Arg Asn Tyr Val Gly Glu His His Asp
100 105 110
His Leu Thr Tyr Leu Met Ala Trp Arg Gln Ser Tyr Asn Lys Lys Gly
115 120 125
Gln Trp Val Asp Asp His Asn Ser Ala Thr Asp Gly Asp Leu Tyr Ile
130 135 140
Ala Ala Ala Leu His Arg Ala Ala Lys Val Trp Pro Gln Lys Ala Pro
145 150 155 160
Tyr Tyr His Lys Leu Glu Gln Gln Ile Ala Thr Asp Ile Leu Lys Tyr
165 170 175
Glu Tyr Asn Pro Thr Thr His Met Leu Thr Val Gly Asp Trp Val Thr
180 185 190
Lys Asp Ser Lys Phe Tyr Tyr Leu Leu Arg Thr Ser Asp Val Met Pro
195 200 205
Thr Val Phe Asp His Leu Tyr Asp Cys Thr His Asp Ser Arg Trp Gln
210 215 220
Met Ile Lys Asn Asn Met Leu Asp Arg Leu Ala Ala Leu Ser Lys Leu
225 230 235 240
His Gln Thr Gly Leu Val Pro Asp Phe Ala Trp Ala Arg Pro Gly Ser
245 250 255
Thr Lys Pro Val Gly Pro Asn Thr Val Ala Gly Lys Tyr Asp Gly Asp
260 265 270
Tyr Ser Ala Asn Ala Cys Arg Val Pro Met Met Leu Ala Lys Ser Asn
275 280 285
Asp Pro Arg Ala Gln Lys Val Leu Asn Lys Met Met Arg Phe Phe Ser
290 295 300
Glu Gln Tyr Tyr Ile Thr Ala Gly Tyr Ser Leu Asn Gly His Arg Leu
305 310 315 320
Val Lys Tyr Gln Ser Asn Ser Phe Ser Ala Pro Ile Phe Tyr Ala Val
325 330 335
Ser Cys Asn Arg Asn Glu Gly Tyr Asp Asn Leu Phe Ala Ser Gln Lys
340 345 350
His Ile Phe Ser Lys Pro Leu Thr Glu Lys Asn Tyr Tyr Asp Ala Thr
355 360 365
Leu Thr Thr Leu Ala Ala Leu Glu Gly Met Asn Asn
370 375 380
<210> 91
<211> 369
<212> PRT
<213> 肠沙门氏菌
<400> 91
Met Met Thr Met Leu Arg Gly Trp Ile Thr Met Ile Val Met Leu Thr
1 5 10 15
Ala Ile Asn Ala Gln Ala Ala Cys Ser Trp Pro Ala Trp Glu Gln Phe
20 25 30
Lys Lys Asp Tyr Ile Ser Gln Gln Gly Arg Val Ile Asp Pro Gly Asp
35 40 45
Ala Arg Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Met Phe Phe
50 55 60
Ala Leu Ala Ala Asn Asp Arg Pro Ala Phe Ala Gln Leu Phe Asn Trp
65 70 75 80
Thr Gln Asn Asn Leu Ala Gln Gly Ser Leu Arg Glu His Leu Pro Ala
85 90 95
Trp Leu Trp Gly Gln Lys Asn Pro Asp Thr Trp Ser Val Leu Asp Ser
100 105 110
Asn Ser Ala Ser Asp Gly Asp Ile Trp Met Ala Trp Ser Leu Leu Glu
115 120 125
Ala Gly Arg Leu Trp Lys Glu Thr Arg Tyr Thr Glu Val Gly Thr Ala
130 135 140
Leu Leu Lys Arg Ile Ala Arg Glu Glu Val Val Asn Val Pro Gly Leu
145 150 155 160
Gly Ser Met Leu Leu Pro Gly Lys Ile Gly Phe Ala Glu Ala Asn Ser
165 170 175
Trp Arg Phe Asn Pro Ser Tyr Leu Pro Pro Gln Leu Ala Gln Tyr Phe
180 185 190
Ser Arg Phe Gly Ala Pro Trp Ser Thr Leu Arg Glu Thr Asn Leu Arg
195 200 205
Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val Arg
210 215 220
Tyr Glu Ser Lys Gln Gly Trp Gln Leu Lys Ala Glu Lys Thr Leu Ile
225 230 235 240
Ser Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met His
245 250 255
Asp Gly Asp Pro Gln Lys Ala Arg Leu Leu Ala Arg Phe Lys Pro Met
260 265 270
Ala Thr Leu Thr Met Lys Asn Gly Val Pro Pro Glu Lys Val Asp Val
275 280 285
Val Ser Gly Asn Ala Gln Gly Thr Gly Pro Val Gly Phe Ser Ala Ala
290 295 300
Leu Leu Pro Phe Leu Gln Asn Arg Asp Ala Gln Ala Val Gln Arg Gln
305 310 315 320
Arg Val Ala Asp His Phe Pro Gly Ser Asp Ala Tyr Tyr Asn Tyr Val
325 330 335
Leu Thr Leu Phe Gly Gln Gly Trp Asp Gln His Arg Phe Arg Phe Thr
340 345 350
Val Lys Gly Glu Leu Leu Pro Asp Trp Gly Gln Glu Cys Val Ser Ser
355 360 365
Arg
<210> 92
<211> 453
<212> PRT
<213> 蜡状芽孢杆菌
<400> 92
Met Asn Gly Lys Arg Asn Ile Phe Thr Cys Ile Ser Ile Val Gly Ile
1 5 10 15
Gly Leu Ala Ser Phe Ser Asn Ser Ser Phe Ala Ala Ser Val Thr Asp
20 25 30
Asn Ser Ile Gln Asn Ser Ile Pro Val Val Asn Gln Gln Val Ala Ala
35 40 45
Ala Lys Glu Met Lys Pro Phe Pro Gln Gln Val Asn Tyr Ala Gly Val
50 55 60
Ile Lys Pro Asn His Val Thr Gln Glu Ser Leu Asn Ala Ser Val Arg
65 70 75 80
Ser Tyr Tyr Asp Asn Trp Lys Lys Lys Tyr Leu Lys Asn Asp Leu Ser
85 90 95
Ser Leu Pro Gly Gly Tyr Tyr Val Lys Gly Glu Ile Thr Gly Asp Ala
100 105 110
Asp Gly Phe Lys Pro Leu Gly Thr Ser Glu Gly Gln Gly Tyr Gly Met
115 120 125
Ile Ile Thr Val Leu Met Ala Gly Tyr Asp Ser Asn Ala Gln Lys Ile
130 135 140
Tyr Asp Gly Leu Phe Lys Thr Ala Arg Thr Phe Lys Ser Ser Gln Asn
145 150 155 160
Pro Asn Leu Met Gly Trp Val Val Ala Asp Ser Lys Lys Ala Gln Gly
165 170 175
His Phe Asp Ser Ala Thr Asp Gly Asp Leu Asp Ile Ala Tyr Ser Leu
180 185 190
Leu Leu Ala His Lys Gln Trp Gly Ser Asn Gly Ala Val Asn Tyr Leu
195 200 205
Lys Glu Ala Gln Asp Met Ile Thr Lys Gly Ile Lys Ala Ser Asn Val
210 215 220
Thr Asn Asn Ser Arg Leu Asn Leu Gly Asp Trp Asp Ser Lys Ser Ser
225 230 235 240
Leu Asp Thr Arg Pro Ser Asp Trp Met Met Ser His Leu Arg Ala Phe
245 250 255
Tyr Glu Phe Thr Gly Asp Lys Thr Trp Leu Thr Val Ile Asn Asn Leu
260 265 270
Tyr Asp Val Tyr Thr Gln Phe Ser Asn Lys Tyr Ser Pro Asn Thr Gly
275 280 285
Leu Ile Ser Asp Phe Val Val Lys Asn Pro Pro Gln Pro Ala Pro Lys
290 295 300
Asp Phe Leu Asn Glu Ser Glu Tyr Thr Asn Ala Tyr Tyr Tyr Asn Ala
305 310 315 320
Ser Arg Val Pro Leu Arg Ile Val Met Asp Tyr Ala Met Tyr Gly Glu
325 330 335
Lys Arg Ser Lys Val Ile Ser Asp Lys Val Ser Ser Trp Ile Gln Asn
340 345 350
Lys Thr Asn Gly Asn Pro Ser Lys Ile Val Asp Gly Tyr Gln Leu Asn
355 360 365
Gly Ser Asn Ile Gly Ser Tyr Pro Thr Ala Val Phe Val Ser Pro Phe
370 375 380
Ile Ala Ala Ser Ile Thr Asn Ser Asn Asn Gln Lys Trp Val Asn Ser
385 390 395 400
Gly Trp Asp Trp Met Lys Asn Lys Arg Glu Ser Tyr Phe Ser Asp Ser
405 410 415
Tyr Asn Leu Leu Thr Met Leu Phe Ile Thr Gly Asn Trp Trp Lys Pro
420 425 430
Ile Pro Asp Asn Lys Lys Ala Gln Asn Gln Ile Asn Asp Ala Ile Tyr
435 440 445
Glu Gly Tyr Asp Asn
450
<210> 93
<211> 412
<212> PRT
<213> 蜡状芽孢杆菌
<400> 93
Met Arg Arg Asn His Asp Phe Asn Asp Ser Gly Lys Lys Arg Leu Glu
1 5 10 15
Lys Val Glu Ser Ile Phe Thr Ile His Lys Pro Phe Pro Gln Gln Val
20 25 30
Thr Tyr Pro Gly Val Val Lys Pro Asn His Ile Ser Gln Glu Glu Leu
35 40 45
Asn Glu Ser Val Lys Ser Tyr Tyr Asn His Trp Lys Met Asn Tyr Leu
50 55 60
Lys Asn Asp Leu Ser Ser Leu Pro Gly Gly Tyr Tyr Val Lys Gly Glu
65 70 75 80
Ile Thr Gly Asn Pro Glu Gly Phe Thr Pro Leu Gly Thr Ser Glu Gly
85 90 95
Gln Gly Tyr Gly Met Val Ile Thr Val Leu Met Ala Gly Tyr Asp Ser
100 105 110
Ala Ala Gln Ser Ile Tyr Asn Gly Leu Phe Lys Thr Val Lys Thr Phe
115 120 125
Lys Ser Ser Lys Asn Ser Asn Leu Met Gly Trp Ile Val Thr Asp Asp
130 135 140
Lys Lys Ala Phe Gly Tyr Phe Pro Ser Ala Thr Asp Gly Asp Leu Asp
145 150 155 160
Ile Ala Tyr Ser Leu Leu Leu Ala His Thr Gln Trp Gly Ser Thr Gly
165 170 175
Ala Ile Asn Tyr Leu Lys Glu Ala Gln Gln Met Ile Thr Glu Gly Ile
180 185 190
Lys Lys Ser Leu Val Thr Ile Asn Lys Arg Leu Asn Leu Gly Asp Trp
195 200 205
Asp Lys Asp Thr Thr Leu Asn Thr Arg Ser Ser Asp Trp Met Met Ser
210 215 220
Asn Phe Arg Ala Phe Tyr Glu Phe Thr Asn Asp Gln Ile Trp Leu Asn
225 230 235 240
Thr Ile Asp Asn Leu Tyr Asn Ile Tyr Glu Glu Phe Ser Thr Lys Tyr
245 250 255
Ser Pro Asn Thr Gly Leu Val Ser Asp Phe Val Val Asp Ser Pro Pro
260 265 270
Gln Pro Ala Ser Glu Asn Phe Leu Asn Glu Ser Glu Tyr Thr Gly Ala
275 280 285
Tyr Tyr Tyr Asn Ala Ser Arg Val Pro Leu Arg Ile Val Met Asp Tyr
290 295 300
Ala Met Tyr Gly Glu Lys Arg Ser Lys Ile Ile Ser Asp Lys Ile Ser
305 310 315 320
Thr Trp Ile Arg Asn Gln Thr Ser Glu Asn Pro Ser Asn Ile Val Asp
325 330 335
Gly Tyr His Leu Asn Gly Ser Val Ile Gly Ser Tyr Ala Thr Ala Val
340 345 350
Phe Val Ser Pro Phe Ile Ala Ala Ser Ile Thr Asn Thr Ser Asn Gln
355 360 365
Asn Trp Ile Asn Ser Gly Trp Asp Trp Met Glu Asn Lys Lys Glu Ser
370 375 380
Tyr Tyr Ser Asp Ser Tyr Asn Leu Leu Thr Met Leu Phe Ile Ser Gly
385 390 395 400
Asn Trp Trp Lys Pro Val Pro Glu Asn Arg Gln Ile
405 410
<210> 94
<211> 477
<212> PRT
<213> Clostridium thermocellum
<400> 94
Met Lys Asn Val Lys Lys Arg Val Gly Val Val Leu Leu Ile Leu Ala
1 5 10 15
Val Leu Gly Val Tyr Met Leu Ala Met Pro Ala Asn Thr Val Ser Ala
20 25 30
Ala Gly Val Pro Phe Asn Thr Lys Tyr Pro Tyr Gly Pro Thr Ser Ile
35 40 45
Ala Asp Asn Gln Ser Glu Val Thr Ala Met Leu Lys Ala Glu Trp Glu
50 55 60
Asp Trp Lys Ser Lys Arg Ile Thr Ser Asn Gly Ala Gly Gly Tyr Lys
65 70 75 80
Arg Val Gln Arg Asp Ala Ser Thr Asn Tyr Asp Thr Val Ser Glu Gly
85 90 95
Met Gly Tyr Gly Leu Leu Leu Ala Val Cys Phe Asn Glu Gln Ala Leu
100 105 110
Phe Asp Asp Leu Tyr Arg Tyr Val Lys Ser His Phe Asn Gly Asn Gly
115 120 125
Leu Met His Trp His Ile Asp Ala Asn Asn Asn Val Thr Ser His Asp
130 135 140
Gly Gly Asp Gly Ala Ala Thr Asp Ala Asp Glu Asp Ile Ala Leu Ala
145 150 155 160
Leu Ile Phe Ala Asp Lys Leu Trp Gly Ser Ser Gly Ala Ile Asn Tyr
165 170 175
Gly Gln Glu Ala Arg Thr Leu Ile Asn Asn Leu Tyr Asn His Cys Val
180 185 190
Glu His Gly Ser Tyr Val Leu Lys Pro Gly Asp Arg Trp Gly Gly Ser
195 200 205
Ser Val Thr Asn Pro Ser Tyr Phe Ala Pro Ala Trp Tyr Lys Val Tyr
210 215 220
Ala Gln Tyr Thr Gly Asp Thr Arg Trp Asn Gln Val Ala Asp Lys Cys
225 230 235 240
Tyr Gln Ile Val Glu Glu Val Lys Lys Tyr Asn Asn Gly Thr Gly Leu
245 250 255
Val Pro Asp Trp Cys Thr Ala Ser Gly Thr Pro Ala Ser Gly Gln Ser
260 265 270
Tyr Asp Tyr Lys Tyr Asp Ala Thr Arg Tyr Gly Trp Arg Thr Ala Val
275 280 285
Asp Tyr Ser Trp Phe Gly Asp Gln Arg Ala Lys Ala Asn Cys Asp Met
290 295 300
Leu Thr Lys Phe Phe Ala Arg Asp Gly Ala Lys Gly Ile Val Asp Gly
305 310 315 320
Tyr Thr Ile Gln Gly Ser Lys Ile Ser Asn Asn His Asn Ala Ser Phe
325 330 335
Ile Gly Pro Val Ala Ala Ala Ser Met Thr Gly Tyr Asp Leu Asn Phe
340 345 350
Ala Lys Glu Leu Tyr Arg Glu Thr Val Ala Val Lys Asp Ser Glu Tyr
355 360 365
Tyr Gly Tyr Tyr Gly Asn Ser Leu Arg Leu Leu Thr Leu Leu Tyr Ile
370 375 380
Thr Gly Asn Phe Pro Asn Pro Leu Ser Asp Leu Ser Gly Gln Pro Thr
385 390 395 400
Pro Pro Ser Asn Pro Thr Pro Ser Leu Pro Pro Gln Val Val Tyr Gly
405 410 415
Asp Val Asn Gly Asp Gly Asn Val Asn Ser Thr Asp Leu Thr Met Leu
420 425 430
Lys Arg Tyr Leu Leu Lys Ser Val Thr Asn Ile Asn Arg Glu Ala Ala
435 440 445
Asp Val Asn Arg Asp Gly Ala Ile Asn Ser Ser Asp Met Thr Ile Leu
450 455 460
Lys Arg Tyr Leu Ile Lys Ser Ile Pro His Leu Pro Tyr
465 470 475
<210> 95
<211> 383
<212> PRT
<213> Burkholderia cenocepacia
<400> 95
Met Thr Cys Ala Ala Ala Gly Met Ala Thr Arg Ala His Ala Ala Gly
1 5 10 15
Thr Gly Ala Ala Asp Ala Ala Gly Ala Gly Cys Ser Val Ala Trp Pro
20 25 30
Arg Trp Asp Ala Phe Lys Arg Asp Phe Ile Ser Ala Asp Gly Arg Val
35 40 45
Ile Asp Val Gly Ser Ala Asp Ser Arg Thr Val Ser Glu Gly Gln Ala
50 55 60
Tyr Gly Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Arg Met Phe Asp
65 70 75 80
Thr Ile Leu Ala Trp Thr Glu Asn Asn Leu Ala Gln Gly Asp Leu Ser
85 90 95
Ala His Leu Pro Ala Trp Leu Trp Gly Arg Ala Pro Asp Gly Ala Trp
100 105 110
Arg Val Leu Asp Ala Asn Pro Ala Ser Asp Ala Asp Leu Trp Ile Ala
115 120 125
Tyr Ala Leu Val Glu Ala Gly Arg Leu Trp Arg Glu Arg Ser Tyr Thr
130 135 140
Ala Arg Gly Ala Leu Leu Ala Lys Arg Val Leu Asp Ala Glu Thr Ala
145 150 155 160
Thr Val Pro Gly Leu Gly Leu Thr Leu Leu Pro Gly Pro Thr Gly Phe
165 170 175
Lys Leu Ala Asp Gly Gln Trp Arg Val Asn Pro Ser Tyr Ser Pro Pro
180 185 190
Gln Val Ile Arg Ala Leu Gly Ala Arg Leu Pro Asp Asp Arg Arg Trp
195 200 205
Ala Ala Leu Ala Ser Ser Thr Gly Arg Val Leu Leu Asp Thr Ala Pro
210 215 220
Lys Gly Phe Ser Pro Asp Trp Ala Leu Tyr Arg Ala Gly Lys Gly Phe
225 230 235 240
Gly Pro Asp Pro Asp Thr His Ala Gln Ser Ala Tyr Asn Ala Ile Arg
245 250 255
Val Tyr Leu Trp Ala Gly Met Leu Asp Arg Ala Asp Pro Leu Ala Ala
260 265 270
Pro Leu Leu Ala Lys Phe Ala Pro Phe Ala Asp Ala Ile Ala Ala His
275 280 285
Gly Ala Pro Pro Glu Arg Val Asp Thr Thr Thr Gly Val Ala Gly Pro
290 295 300
Asn Asp Gly Asn Gly Gly Phe Ser Ala Ala Ala Val Pro Phe Leu Asp
305 310 315 320
Ala Arg Gly Gln His Ala Leu Ala Asp Ala Gln Ala Ala Arg Val Asp
325 330 335
Thr Leu Ala Arg Gln Ser Ala Pro Gly Tyr Tyr Thr Ser Val Leu Thr
340 345 350
Leu Phe Gly Leu Gly Trp Arg Asp Gly Arg Tyr Arg Phe Gly Ala Asp
355 360 365
Gly Thr Leu Asp Thr Arg Trp Gly Asp Arg Ser Cys Ala Ala Arg
370 375 380
<210> 96
<211> 345
<212> PRT
<213> 奇异变形菌
<400> 96
Met Ser Ile Arg Gln Leu Ser Arg Leu Thr His Arg Leu Gly Ile Leu
1 5 10 15
Leu Thr Leu Ser Val Met Leu Met Ile Ser Pro Met Thr Gln Ala Thr
20 25 30
Glu Thr Gln Ala Gly Trp Gln Gln Phe Lys Ala Arg Tyr Ile Thr Pro
35 40 45
Glu Gly Arg Val Ile Asp Ser Ala Asn Gln Asn Ile Ser His Ser Glu
50 55 60
Gly Gln Gly Tyr Gly Met Leu Met Ala Val Met Ser Asp Asp Arg Gln
65 70 75 80
Thr Phe Ala Gln Leu Trp His Trp Thr Ala Met Ser Leu Tyr Arg Gly
85 90 95
Asp Leu Gly Leu Phe Lys Trp Arg Tyr Glu Pro Glu Asn Asn Gln His
100 105 110
Thr Pro Asp Pro Asn Asn Ala Thr Asp Gly Asp Ile Leu Ile Ala Trp
115 120 125
Ala Leu Leu Lys Ala Gly Glu Lys Trp His Asp Glu Ser Tyr Leu Ser
130 135 140
Ala Ser Asp Ser Ile Gln His Ala Ile Leu Glu His Thr Leu Val Lys
145 150 155 160
Thr Glu Asn Tyr Ser Val Leu Leu Pro Gly Ile Asn Gly Phe Lys Thr
165 170 175
Pro Glu Glu Ile Ile Ile Asn Pro Ser Tyr Phe Ile Phe Pro Ala Trp
180 185 190
Lys Asp Phe Tyr Arg Val Ser His Asp Ser Arg Trp Lys Asn Leu Ile
195 200 205
Asn Asp Ser Gln Ser Leu Leu Arg Lys Met Arg Phe Gly Lys Tyr Lys
210 215 220
Leu Pro Ser Asp Trp Val Ser Leu Tyr Pro Asp Gly His Leu Ala Pro
225 230 235 240
Ser Glu Lys Trp Pro Ala Arg Phe Ser Phe Asp Ala Ile Arg Ile Pro
245 250 255
Leu Tyr Leu Ala Trp Ala Gln Asp Lys Leu Ala Leu Gln Pro Phe Val
260 265 270
Asn Tyr Trp Gln Gln Phe Asp Arg Asp Lys Thr Pro Ala Trp Ile Ser
275 280 285
Ile Asp Gly Lys Glu Arg Ala Asp Tyr Asn Leu Thr Pro Gly Met Met
290 295 300
Ala Val Arg Asp Leu Thr Met Lys Thr Val Ile Glu Asn Val Asp Leu
305 310 315 320
Thr Lys Asp Thr Asp Tyr Tyr Ser Ser Ala Leu His Leu Leu Ala Ala
325 330 335
Phe Ala Gln Asn Asn His Asn Asp Tyr
340 345
<210> 97
<211> 366
<212> PRT
<213> 麦氏交替单胞菌
<400> 97
Met Val Thr Gly Phe Lys Ala Thr Lys Thr Leu Leu Ala Ile Ala Leu
1 5 10 15
Thr Leu Leu Leu Val Ser Cys Ser Thr Lys Glu Asp Asn Ala Phe Lys
20 25 30
Ser Gln Phe Met Ala Tyr Lys Ala Leu Phe Ile Asp Gly Gly Arg Val
35 40 45
Val Asp Thr Gly Asn Asp Glu Val Ser His Ser Glu Gly Gln Gly Tyr
50 55 60
Gly Met Leu Phe Ala Val Ala Ala Asp Asp Lys Asp Thr Phe Asp Ala
65 70 75 80
Leu Trp His Trp Thr Gln Arg Thr Leu Leu Arg Ser Asp Gly Leu Phe
85 90 95
Ser Trp Arg Tyr Arg Pro Cys Ala Asp Asn Ser Glu Ser Cys Ile Asp
100 105 110
Asp Pro Asn Asn Ala Ser Asp Gly Glu Ile Leu Ile Ala Trp Ala Leu
115 120 125
Leu Arg Ala Ser Glu Lys Trp Gly Ile Lys Gly Tyr Gln Asp Glu Ala
130 135 140
Gly Lys Ile Val Gln Ala Val Glu Gln Lys Leu Leu Val Glu His Gln
145 150 155 160
Gly Ser Val Met Leu Leu Pro Gly Glu Tyr Gly Phe Thr Ser Gln Ser
165 170 175
Glu Gly Gln Lys Ser Leu Gln Leu Asn Leu Ser Tyr Trp Val Phe Pro
180 185 190
Ala Leu Thr Asp Leu Ser Ala Leu Ser Asn Thr Pro Ser Arg Trp Gln
195 200 205
Lys Leu His Gln Thr Gly Leu Thr Leu Leu Ser Asn Met Gln Phe Ser
210 215 220
Ser Tyr Gln Leu Pro Ser Asp Trp Val Arg Phe Glu Pro Arg Asn Ser
225 230 235 240
Gly Ser Ala Lys Ser Gly Leu Ser Gly Glu Leu Thr Leu Thr Asn Val
245 250 255
Val Ser Ala Glu Phe Gly Phe Asn Ala Ile Arg Ile Pro Leu Gln Leu
260 265 270
Ala Trp Ser Val Gln Val Arg Asp Asn Ala Ala Leu Ala Glu Glu Leu
275 280 285
Phe Ala Pro Tyr Tyr Gln Trp Trp Ala Lys Thr Pro Thr Pro Ala Thr
290 295 300
Val Asn Leu Leu Thr Glu Gln Thr Ala Glu Tyr Glu Met Thr Pro Gly
305 310 315 320
Met Gln Ser Val Lys Leu Ala Val Lys Tyr Leu Met Lys Ser Glu Glu
325 330 335
Pro Val Trp Pro Thr Val Asn Arg Lys Met Asp Tyr Tyr Ser Ala Ser
340 345 350
Leu Thr Leu Leu Ser Met Leu Ala Val Ser Asp Asn Ala Ser
355 360 365
<210> 98
<211> 1140
<212> PRT
<213> Anaeromyxobacter
<400> 98
Met Arg Pro Thr Ala Arg Asn Leu Ala Pro Leu Leu Ala Val Leu Cys
1 5 10 15
Ala Pro Pro Ala Leu Leu Gly Gly Cys Gly His Ala Glu Ala Arg Pro
20 25 30
Arg Ala Ala Ala Gln Asp Arg Asp Gly Leu Ser Ala Leu Trp Ser Tyr
35 40 45
Tyr Arg Tyr Thr His Val Gln Gly Gly Arg Val Ala Ala Leu Asp Glu
50 55 60
Gly Gly Val Thr Thr Ser Glu Gly Gln Gly Tyr Ala Met Leu Arg Ala
65 70 75 80
Val Trp Met Asp Asp Arg Ala Thr Leu Asp Ala Val Trp Arg Trp Thr
85 90 95
Arg Glu Asn Leu Ala Val Arg Glu Asp Arg Leu Leu Ala Trp Arg Trp
100 105 110
Leu Gly Gly Lys Val Gln Asp Arg Asn Ala Ala Thr Asp Ala Asp Gln
115 120 125
Asp Val Ala Leu Ala Leu Val Leu Ala Ser Arg Arg Phe Gly Asp Pro
130 135 140
Arg Tyr Leu Glu Ala Ala Arg Pro Leu Leu Ala Asp Ile Trp Ala Arg
145 150 155 160
Glu Val Ile Arg Ala Gly Gly Arg Trp Leu Pro Thr Gly Gly Asn Trp
165 170 175
Ala Pro Ala Glu Arg Tyr Pro Thr Ile His Val Ala Tyr Leu Ala Pro
180 185 190
Tyr Ala Tyr Gln Val Phe Ala Asp Val Asp Pro Glu Arg Pro Trp Ala
195 200 205
Glu Leu Val Glu Ser Ser Tyr Gln Val Leu Arg Phe Leu Met Ile Glu
210 215 220
Lys Gly Leu Ala Leu Pro Pro Glu Arg Ile Trp Val Asp Pro Arg Thr
225 230 235 240
Gly Glu Leu Leu Leu Ala Arg Pro Gly Gly Ala Pro Asp Ala Phe Gly
245 250 255
Tyr Asp Ala Val Pro Ile Tyr Trp Arg Val Ala Leu Asp Ala Arg Trp
260 265 270
Phe Gly Arg Arg Gly Glu Ala Glu Leu Arg Ala Arg Leu Leu Arg Phe
275 280 285
Pro Ala Glu Ala Phe Arg Asp Glu Gly Arg Leu Arg Glu Arg Tyr Thr
290 295 300
Thr Ser Gly Arg Pro Leu Ser Gly Leu Asp Gly Leu Pro His Leu Ala
305 310 315 320
Ala Val Gln Ala Leu Ala Gln Val Glu Asp Pro Glu Leu Ala Arg Ala
325 330 335
Met Arg Ala Ala Ser Leu Asp Ala Leu Phe Asp Arg Ala Leu Ser Gly
340 345 350
Glu Ala Thr Pro Tyr Tyr Leu His Asn Trp Leu Trp Phe Gly Arg Ala
355 360 365
Leu Glu Ala Gly Leu Leu Arg Arg Phe Asp Glu Pro Leu Ala Phe Leu
370 375 380
Ser Ser Leu Asp Trp Gly Ala Phe Arg Asp Arg Phe Pro Leu Leu Ala
385 390 395 400
Val Gly Leu Ala Leu Leu Leu Ala Pro Leu Ala Arg Arg Leu Trp Pro
405 410 415
Ala Arg Ala Ala Leu Val Ala Leu Ala Leu Gly Leu Ser Val Arg Tyr
420 425 430
Leu Ala Trp Arg Ala Gly Ala Thr Leu Asn Phe Val Glu Pro Leu Gly
435 440 445
Pro Leu Leu Ser Leu Ser Leu Leu Ala Ala Glu Ala Tyr Ala Leu Ser
450 455 460
Thr Val Ala Leu Leu Ala Val Gln Val Gly Leu Arg His Arg Ala Arg
465 470 475 480
Pro Pro Glu Pro Pro Leu Gly Pro Glu Glu Pro His Pro Ser Val Asp
485 490 495
Val Met Val Pro Ile Tyr Ser Glu Pro Leu Asp Ile Leu Asp Lys Thr
500 505 510
Leu Ala Ala Cys Ala Ala Met Arg Tyr Pro Arg Lys Thr Val Val Val
515 520 525
Cys Asp Asp Ser His Arg Asp Glu Val Ala Arg Leu Ala Ala Glu His
530 535 540
Gly Ala Arg Tyr Leu Ala Gly Pro Arg Arg His Ala Lys Ala Gly Asn
545 550 555 560
Leu Asn Ala Ala Leu Ala Arg Thr Asp Gly Glu Leu Val Val Val Phe
565 570 575
Asp Thr Asp His Val Pro Thr Ala Ala Phe Leu Glu Arg Thr Val Pro
580 585 590
Cys Phe Arg Asp Pro Arg Leu Gly Val Val Gln Thr Pro His His Phe
595 600 605
Tyr Asn Pro Asp Val Phe Gln Arg Ala Leu Gly Ala Pro Ala Ala Val
610 615 620
Pro Asn Glu Ala Asp Leu Phe Asn His Ala Ile Gln Gly Gly Arg Asp
625 630 635 640
Arg Trp Gly Gly Ala Phe Phe Val Gly Ser Gly Ala Val Phe Arg Arg
645 650 655
Glu Ala Leu Ala Ser Val Gly Gly Phe His Leu Leu Ser Ile Thr Glu
660 665 670
Asp Ile His Thr Ser Gln Lys Leu His Ala Arg Gly Trp Arg Thr Arg
675 680 685
Phe Val Asp Glu Asp Leu Ala Ala Gly Leu Ser Ala Glu Asp Leu Ala
690 695 700
Ser Tyr Val Val Gln Arg Arg Arg Trp Met Leu Gly Cys Leu Gln Ile
705 710 715 720
Phe Phe Arg Asp Asn Pro Leu Leu Gln Arg Gly Leu Pro Leu Arg His
725 730 735
Arg Val Gly Tyr Leu Ala Ser Leu Trp Tyr Phe Phe Phe Pro Leu Ala
740 745 750
Arg Leu Val Phe Phe Ala Thr Pro Leu Ala Tyr Leu Leu Phe His Leu
755 760 765
His Pro Leu Phe Ala Glu Leu Pro Val Leu Leu Ala Tyr Leu Val Pro
770 775 780
His Leu Val Ala Ala Pro Leu Ala Ala Ser Ala Leu Val Pro Gly Trp
785 790 795 800
Pro Arg Ala Leu Trp Gly Ser Leu Tyr Glu Gly Ala Val Ala Phe Pro
805 810 815
Leu Ala Arg Ala Thr Leu Asp Leu Leu Leu Pro Arg Arg Leu Gly Phe
820 825 830
Lys Val Thr Pro Lys Gly Val Arg Ser Glu Arg Arg Arg Phe Asp Leu
835 840 845
Arg Thr Ser Ala Phe Thr Leu Ala Ala Ala Gly Val Gly Leu Leu Ala
850 855 860
Ile Ala Lys Gly Ala Ala Glu Leu Trp Ala Phe Gly Ile Glu Val Glu
865 870 875 880
Ala Tyr Ala Phe Asn Leu Ala Trp Ala Ala Ala Asn Leu Val Ser Val
885 890 895
Leu Leu Ala Leu Val Val Ala Trp Glu Arg Pro Gln Arg Arg Glu Asp
900 905 910
Glu Arg Ile Arg Arg Arg Val Pro Val Arg Leu Glu Ala Pro Gly Leu
915 920 925
Ser Leu Asp Ala Thr Thr Leu Asp Leu Ala Arg Gly Gly Ala Ala Val
930 935 940
Ala Leu Pro Pro Gly Ala Ala Leu Pro Ala Glu Val Glu Val Ala Phe
945 950 955 960
Gly Ala Pro Glu Pro Leu Arg Leu Arg Ala Arg Val Ala Tyr Leu Glu
965 970 975
Ala Val Gly Gly Thr Pro Arg Ala Gly Leu Ala Phe Gln Gly Val Ser
980 985 990
Ala Ala Asp Ala Arg Ala Leu Val Arg Ile Ala Phe Ser Ala Asp Gly
995 1000 1005
Ala His Ala Gly Ala His Ala Gly Arg Ser Arg Trp Gln Val Ala
1010 1015 1020
Met Ala Trp His Leu Leu Ala Gly Leu Trp Arg Ala Val Arg Pro
1025 1030 1035
Leu Arg Ala Arg Arg Arg Leu Gly Ser Arg Arg Arg Thr Leu Arg
1040 1045 1050
Pro Leu Arg Leu Cys Gly Ala Gly Arg Ala Cys Arg Ala Val Ala
1055 1060 1065
Val Asp Ala Ser Pro Gly Gly Leu Gly Leu Val Ala Ala Gly Pro
1070 1075 1080
Gly Asp Ala Met Pro Ala Pro Gly Thr Ser Leu Ala Leu Leu Ala
1085 1090 1095
Pro Glu Gly Ile Ala Trp Ala Arg Val Ala His Ala His Arg Ile
1100 1105 1110
Leu Pro Gly Leu Trp Arg Leu Gly Leu Thr Leu Gln Ala Glu Pro
1115 1120 1125
Val Pro Gly Ala Glu Pro His Ala Tyr Leu Ala Ala
1130 1135 1140
<210> 99
<211> 454
<212> PRT
<213> 原型体微鞘蓝细菌
<400> 99
Met Thr Asn Asp Lys Gly Gln Arg Thr Asn His Trp Val Arg Ser Arg
1 5 10 15
His Phe Ser Ala Arg Ser Ala Lys Ala Leu Thr Thr Ser Met Thr Ile
20 25 30
Ser Ala Met Leu Leu Leu Leu Asn Leu Thr Ser Cys Val Ser Ile Lys
35 40 45
Phe Ser Glu Pro Glu Pro Pro Glu Ala Ser Val Ser Pro Ser Pro Ser
50 55 60
Pro Val Thr Ala Ser Pro Val Ala Asp Phe Pro Val Leu Ser Pro Ser
65 70 75 80
Pro Pro Ile Gln Asp Ile Leu Glu Gln Ser Trp Val Ile Tyr Arg Gln
85 90 95
Gln Phe Ile Gln Glu Asp Gly Arg Val Ile Asp Tyr Glu Ala Ser Asp
100 105 110
Arg Ser Thr Ser Glu Gly Gln Ala Tyr Ala Met Leu Arg Ala Val Leu
115 120 125
Ile Asn Asp Pro Thr Thr Phe Ala Leu Thr Leu Glu Trp Ala Glu Asp
130 135 140
Asn Leu His Arg Leu Thr Glu Thr Gly Glu Pro Glu Asp Asn Leu Trp
145 150 155 160
Val Trp Leu Trp Gly Gln Asp Asp Glu Gly Asn Trp Gly Ala Ile Asp
165 170 175
Arg Asn Phe Ala Ser Asp Ala Asp Ile Asp Ala Ile Thr Ala Leu Ile
180 185 190
Trp Ala Tyr Arg Arg Trp Asp Arg Pro Glu Tyr Leu Glu Leu Ala Arg
195 200 205
Ile Lys Leu Arg Asp Leu Trp Asp Tyr Ser Thr Ile Ala Gly Pro Asp
210 215 220
Gly Lys Arg Tyr Leu Leu Pro Gly Pro Lys Gln Ala Phe Val Pro Ser
225 230 235 240
Pro Ser Thr Ile Tyr Leu Asn Pro Ser Tyr Leu Ala Pro Tyr Ala Phe
245 250 255
Arg Leu Phe Ala Gln Val Asp Pro Glu Arg Asp Trp Leu Ser Leu Ile
260 265 270
Asp Ser Ser Tyr Tyr Val Leu Glu Asn Ser Ser Lys Val Ser Ala Val
275 280 285
Gly Leu Pro Ser Asp Trp Ile Ala Leu Asp Leu Gln Thr Asn Gln Phe
290 295 300
Gln Ser Ile Pro Pro Gln Ser Lys Leu Lys Ser Val Tyr Ser Phe Asp
305 310 315 320
Ala Tyr Arg Val Trp Trp Arg Ile Ala Trp Asp Ala Glu Trp Phe Gln
325 330 335
Ser Thr Glu Ala Leu Asn Tyr Leu Glu Thr Ala Thr Gln Tyr Leu Glu
340 345 350
Asp Gln Trp Gln Ser Ser Ser Arg Leu Pro Ala Arg Ile Asn Leu Asp
355 360 365
Gly Glu Ser Leu Val Lys Tyr Asp Ala Thr Ser Gln Tyr Ala Met Leu
370 375 380
Tyr Pro Ala Met Arg Leu Ile Asn Pro Ala Ile Ala Glu Gln Leu Leu
385 390 395 400
Glu Arg Lys Leu Leu Pro Gln Tyr Asn Gln Gly Ile Trp Asp Asp Glu
405 410 415
Ser Ala Tyr Tyr Thr Gln Asn Leu Ala Trp Ile Ala Leu Val Ser Pro
420 425 430
Asp Leu Val Lys Asn Ser Gln Phe Phe Gln Ser Gln Gln Gln Phe Ser
435 440 445
Arg Asn Ala Ile Arg Glu
450
<210> 100
<211> 326
<212> PRT
<213> 微杆菌科
<400> 100
Met Leu Ala Leu Ala Leu Leu Leu Pro Val Ala Gly His Ala Gly Asp
1 5 10 15
Ala Arg Trp Asp Asp Phe Val Ser Arg Phe Val Ala Ala Asp Gly Arg
20 25 30
Val Ile Asp Thr Gly Asn Gly Gly Ile Ser His Ser Glu Gly Gln Gly
35 40 45
Tyr Thr Leu Leu Leu Ala Val Ala Gln Asn Asp Arg Ala Thr Phe Gly
50 55 60
Arg Val Trp Ala Trp Thr Arg Ser Arg Leu Gln Thr Arg Gly Asp Ala
65 70 75 80
Leu Phe Ser Trp Gln Trp Lys Pro Asp Ala Asp Gly Gly Lys Ile Gly
85 90 95
Asp Arg Asn Asn Ala Ser Asp Gly Asp Leu Leu Ile Ala Trp Ala Leu
100 105 110
Leu Arg Gly Ala Gln Arg Trp Gln Gln Pro Ala Tyr Arg Ala Glu Ala
115 120 125
Leu Lys Ile Leu Ala Asp Val Arg Gly Lys Leu Ile Lys Pro Ser Arg
130 135 140
Phe Gly Pro Leu Leu Met Pro Ala Glu Lys Gly Phe Asp Lys Pro Ala
145 150 155 160
Gly Pro Ile Val Asn Pro Ser Tyr Trp Leu Phe Pro Ala Phe Ala Glu
165 170 175
Phe Ala Arg Ala Asp Arg Ala Pro Val Trp Ala Glu Leu Arg Gln Ser
180 185 190
Gly Leu Ala Leu Leu Ala Ala Gly Arg Phe Gly Gln Trp Gln Leu Pro
195 200 205
Pro Asp Trp Leu Gln Val Gly Asp Thr Leu Ala Pro Ala Met Ser His
210 215 220
Gln Tyr Gly Tyr Asn Ala Leu Arg Val Pro Leu Tyr Leu Ala Trp Ala
225 230 235 240
Gly Leu Asp Thr Pro Glu Arg Ile Ala Pro Phe Leu Ala Tyr Trp Asp
245 250 255
Thr Asn Pro Glu Arg Leu Pro Ala Trp Thr Asp Leu Ala Thr Asn Ala
260 265 270
Ile Asp Pro Asp Ser Ala Gln Pro Gly Leu Tyr Thr Ile Val Ala Leu
275 280 285
Val Arg Gln Arg Gln Ala Gly Gly Thr Val Arg Phe Gly Asp Gly Asp
290 295 300
Asn Arg Asp Tyr Tyr Pro Ala Val Leu Asp Leu Leu Ser Arg Gln Ala
305 310 315 320
Ala Arg Glu Ala Arg Arg
325
<210> 101
<211> 387
<212> PRT
<213> Verrucomicrobiae
<400> 101
Met Leu Leu Leu Thr Ser Cys Ala Pro Glu Pro Gln Ile Lys Pro Gln
1 5 10 15
Thr Asp Gln Arg Asn Leu Phe Ala Glu Tyr Leu Gly Lys Ser Gln Gln
20 25 30
Glu Thr Asp Ala Lys Val Glu Asp Ala Phe Gln Gln Leu Phe Tyr Gly
35 40 45
Asp Asp Glu Ser Glu Arg Ile Tyr Tyr Pro Val Gly Asp Asp Met Ala
50 55 60
Tyr Ile Trp Asp Val Gly Ser Asp Asp Val Arg Ser Glu Gly Met Ser
65 70 75 80
Tyr Gly Met Met Ile Ala Val Gln Leu Asp Lys Lys Asp Glu Phe Asp
85 90 95
Arg Leu Trp Lys Trp Ser His Thr Tyr Met Arg Leu His Glu Gly Pro
100 105 110
Phe Glu Gly Tyr Phe Asn Trp Gln Asn Ala Thr Ser Gly Glu Lys Ile
115 120 125
Ser Pro Gly Ser Ala Ser Asp Gly Glu Glu Trp Phe Ala Met Ala Leu
130 135 140
Phe Phe Ala Ser Asn Leu Trp Gly Asp Gly Glu Gly Ile Phe Asn Tyr
145 150 155 160
Arg Ala Glu Ala Asn Asn Ile Leu Arg Glu Met Leu His Lys Gln Pro
165 170 175
Ser Asp Pro Lys Met Val Ser Ile Phe Asp Tyr Glu Arg His Met Val
180 185 190
Arg Phe Val Pro Trp Thr Asp Trp Asp Gly Val Thr Asp Ala Ser Tyr
195 200 205
His Leu Pro Ala Phe Tyr Glu Leu Trp Ala Lys Trp Ala Asp Ser Asp
210 215 220
Asn Asp Tyr Trp Ala Lys Thr Ala Asp Val Ser Arg Glu Phe Phe Lys
225 230 235 240
Thr Ala Ala His Pro Glu Thr Gly Leu Met Pro Asp Tyr Ser Tyr Tyr
245 250 255
Lys Glu Thr Glu Arg Gln Ile Gly Ser His Ala Asp Phe Arg Phe Asp
260 265 270
Ala Trp Arg Val Met Pro Asn Val Ala Leu Asp His Asp Trp Trp Gly
275 280 285
Lys Asp Ala Gln Trp Gln Thr Met Gln Ser Asp Arg Ile Leu Lys Phe
290 295 300
Leu Gly Ser His Leu Pro Asp Ile Pro Asn Gln Phe Ala Val Asp Gly
305 310 315 320
Thr Pro Leu Asp Thr Gln Ser Ser Pro Gly Leu Tyr Ala Met Ala Ala
325 330 335
Thr Ala Gly Leu Ala Ala Asn Arg Asp Val Ala Glu Pro Phe Val Gln
340 345 350
Arg Leu Trp Asp Gln Pro Val Pro Thr Gly His Trp Arg Tyr Tyr Asp
355 360 365
Gly Val Ile His Met Leu Gly Leu Leu Gln Thr Ser Gly His Phe Lys
370 375 380
Ile Ile Glu
385
<210> 102
<211> 431
<212> PRT
<213> Verrucomicrobiae
<400> 102
Met Lys Thr Ile Gly Ile Leu Ala Val Leu Leu Trp Val Ser Ala Val
1 5 10 15
Thr Ala Ala Pro Pro Pro Gly Gly Ala Ala Leu Thr Gly Glu Tyr Pro
20 25 30
Asn Leu Phe Lys Thr Tyr Leu Gly Lys Thr Asp Ala Glu Ile Glu Ala
35 40 45
Lys Val Glu Ala Ala Phe Asn Gln Leu Phe Tyr Gly Asp Pro Phe Thr
50 55 60
Glu Arg Val Tyr Tyr Thr Ala Ser Glu Asp Thr Ala Tyr Leu Ala Asp
65 70 75 80
Val Gly Ser Arg Asp Val Arg Ser Glu Gly Ile Ser Tyr Gly Met Met
85 90 95
Ile Thr Val Gln Met Asp Lys Gln Lys Glu Phe Asn Ala Leu Trp Lys
100 105 110
Trp Ala Lys Val His Met Gln Tyr Thr Glu Gly Pro Leu Arg Gly Tyr
115 120 125
Phe Ala Trp His Arg Asp Tyr Asp Gly Asp Met Thr Arg Ala Asp Gly
130 135 140
Ser Glu Ile Arg Gly Gly Gly Pro Ala Pro Asp Gly Glu Asn Trp Met
145 150 155 160
Val Met Ala Leu Phe Phe Ala Ser His Arg Trp Gly Asp Gly Glu Gly
165 170 175
Ile Phe Asn Tyr Gly Gln Glu Ala Gln Glu Leu Leu Arg Met Met Ile
180 185 190
His Lys Asp Asp Glu Pro Asp Arg Gly Asp Ile Val Ser Met Phe His
195 200 205
Pro Glu Glu Lys Gln Ile Arg Phe Thr Pro Asp Gly Glu Trp Gly Pro
210 215 220
Lys Phe Thr Asp Pro Ser Tyr His Thr Pro His Phe Thr Glu Leu Met
225 230 235 240
Ala Arg Trp Ala Glu Asn Pro Ala Asp Arg Ala Phe Leu Ala Glu Val
245 250 255
Thr Gln Thr Ser Arg Gln Leu Phe Arg Asn Ala Ala His Pro Glu Thr
260 265 270
Gly Ile Met Ala Asn Tyr Thr Glu Phe Asp Gly Thr Pro Ile Val Arg
275 280 285
Trp Asp Arg Ala Phe Ala Ala Asp Ala Trp Arg Thr Leu Gly Trp Pro
290 295 300
Ala Met Asp Trp Ser Trp Trp Gly Glu Asp Glu Trp Met Val Glu Gln
305 310 315 320
Ser Asn Arg Ile Leu Gly Phe Tyr Ala Ala Gln Pro Glu Asp Asp Trp
325 330 335
Pro Asp His Met Glu Leu Asp Gly Thr Val Gln Arg Arg Asn Gly Trp
340 345 350
Ala His Gly Leu Ser Gly Met Ala Ala Thr Ala Ala Leu Ala Ala Asp
355 360 365
Pro Glu Leu Gly Lys Pro Met Val Glu Arg Leu Trp Asn Leu Glu Leu
370 375 380
Pro Asn Asp Val Gly Arg Asp Thr Asp Gly Leu Met Ala Glu Gly Glu
385 390 395 400
Leu Arg Ser His Arg Tyr Tyr Ser Gly Leu Leu Met Met Phe Gly Leu
405 410 415
Leu His Ala Ser Gly Asn Phe Gln Val Tyr Gly Pro Val Glu Pro
420 425 430
<210> 103
<211> 385
<212> PRT
<213> Methylobacterium chloromethanicum
<400> 103
Met Met Phe Gly Arg Pro Arg Ala Ile Ala Ala Ser Leu Leu Leu Gly
1 5 10 15
Leu Thr Leu Ala Pro Leu Gln Ala Met Ala Glu Pro Ala Pro Ile Glu
20 25 30
Ala Lys Pro Ala Ala Gly Ala Thr Val Gly Lys Ala Ala Ser Val Thr
35 40 45
Ala Glu Thr Leu Pro Met Leu Asn Ser Leu Gly Gln Ala Gly Ala Trp
50 55 60
Arg Ser Tyr Lys Ala Arg Phe Val Thr Asp Gln Gly Arg Val Val Asp
65 70 75 80
Thr Ala Asn Gly Arg Ile Ser His Ser Glu Ser Gln Gly Tyr Gly Leu
85 90 95
Leu Leu Ala Val Ala Ala Gly Asp Arg Asp Thr Phe Gln Arg Ile Trp
100 105 110
Asn Trp Thr Arg Ala Asn Leu Met Val Arg Asp Asp Ala Leu Leu Ala
115 120 125
Trp Arg Trp Glu Pro Asp Lys Arg Pro Ala Val Ala Asp Met Asn Asp
130 135 140
Ala Thr Asp Gly Asp Ile Leu Val Ala Trp Ala Leu Val Glu Ala Gly
145 150 155 160
Glu Gly Trp Ala Asp Asp Ser Tyr Arg Leu Ala Ala Arg Arg Ile Ala
165 170 175
Val Asp Ile Ala Arg Arg Thr Val Leu Phe Arg Thr Glu Gly Pro Pro
180 185 190
Leu Leu Leu Pro Ala Met Ser Gly Phe Ser Ala Glu Asp Arg Pro Asp
195 200 205
Gly Pro Val Ile Asn Leu Ser Tyr Trp Ile Phe Pro Ala Phe Pro Arg
210 215 220
Leu Ala Ala Val Ala Pro Glu Phe Asp Trp Asp Arg Leu Gly Ala Thr
225 230 235 240
Gly Arg Asp Leu Val Leu Arg Ala Arg Phe Gly Asp Ala Lys Leu Pro
245 250 255
Thr Glu Trp Ile Ser Met Arg Gly Gly Gln Pro Gln Pro Ala Ser Gly
260 265 270
Phe Pro Pro His Phe Ser Tyr Asn Ala Leu Arg Val Pro Leu Tyr Leu
275 280 285
Ala Met Ala Gly Ile Ser Glu Arg Arg Tyr Tyr Glu Pro Leu Leu Ala
290 295 300
Leu Trp Gly Glu Pro Asp Pro Ala Gly Leu Pro Ile Ile Asp Thr Glu
305 310 315 320
Gly Gly Ser Val Ala Gly Arg Met Ala Glu Pro Gly Tyr Ala Ile Ile
325 330 335
Pro Ala Leu Ala Ala Cys Ala Val Thr Gly Ala Pro Leu Pro Ala Gly
340 345 350
Leu Gln Asp Pro Ala Thr Asp Glu Asn Tyr Tyr Pro Ala Thr Leu His
355 360 365
Leu Leu Ala Leu Thr Ala Ala Asn Met Arg Tyr Arg Pro Cys Leu Gly
370 375 380
Arg
385
<210> 104
<211> 376
<212> PRT
<213> 解纤维梭菌
<400> 104
Met Ser Lys Gly Ala Tyr Phe Thr Lys Gln Tyr Pro Asn Leu Phe Ala
1 5 10 15
Glu Leu Gly Ile Ser Asp Glu Gln Ile Asn Lys Lys Val Asn Asp Thr
20 25 30
Phe Asn Thr Met Phe Phe Asp Pro Glu Glu Lys Ile Tyr Phe Glu Ile
35 40 45
Gly Lys Asp Met Gly Tyr Met Met Asp Thr Gly Asn Asn Asp Ala Arg
50 55 60
Thr Glu Gly Met Ser Tyr Gly Met Met Met Thr Leu Gln Met Asp Arg
65 70 75 80
Lys Asp Ile Phe Asp Arg Leu Trp Leu Phe Ser Lys Thr Tyr Met Tyr
85 90 95
Gln Asn Glu Gly Lys Tyr Gln Gly Tyr Phe Ala Trp Ser Val Ser Thr
100 105 110
Asp Gly Lys Lys Asn Ala Glu Gly Pro Ala Pro Asp Gly Glu Glu Tyr
115 120 125
Phe Ala Met Ala Leu Phe Phe Ala Gly Lys Arg Trp Gly Asp Gly Lys
130 135 140
Pro Pro Phe Asp Tyr Ser Ile Gln Ala Arg Asp Ile Leu Lys His Cys
145 150 155 160
Ile His Gln Ser Glu Ile Val Glu Gly Gly Glu Pro Met Trp Asp Ser
165 170 175
Thr Asn His Tyr Ile Lys Phe Val Pro Glu Thr Pro Phe Ser Asp Pro
180 185 190
Ser Tyr His Leu Pro His Phe Tyr Glu Leu Phe Ala Leu Leu Ala Asn
195 200 205
Glu Glu Asp Lys Asp Phe Trp Lys Lys Ala Ala Glu Ala Ser Arg Asn
210 215 220
Tyr Leu His Ile Ser Cys Asp Arg Asp Thr Gly Met Ala Ser Glu Tyr
225 230 235 240
Ala Glu Phe Asp Gly Thr Pro Lys Lys Leu Phe Arg Asp Phe Gln Phe
245 250 255
Tyr Ser Asp Ser Tyr Arg Val Ala Met Asn Ile Gly Leu Asp Ala Ala
260 265 270
Trp Phe Ser Lys Asp Glu Ser Leu Gly Asp Ile Val Asp Lys Leu Gln
275 280 285
Ser Phe Phe Ser Glu Asn Thr Val Leu Gly Glu Tyr Lys Ala Tyr Thr
290 295 300
Val Lys Gly Glu Pro Phe Asp Ala Pro Ala Met His Pro Val Ala Ile
305 310 315 320
Ile Ala Thr Asn Ala Ala Gly Ser Leu Ala Ala Lys Gly Lys Tyr Arg
325 330 335
Asp Gln Trp Val Lys Asp Phe Trp Glu Leu Pro Leu Arg Lys Gly Val
340 345 350
His Arg Tyr Tyr Asp Asn Cys Leu Tyr Phe Phe Ser Leu Leu Met Leu
355 360 365
Ala Gly Lys Tyr Lys Ile Tyr Ile
370 375
<210> 105
<211> 477
<212> PRT
<213> 解纤维梭菌
<400> 105
Met Lys Lys Phe Phe Ala Cys Leu Leu Val Leu Ala Ile Ile Val Thr
1 5 10 15
Ile Val Pro Ile Asn Ile Ala Ser Ala Glu Thr Gly Gly Tyr Tyr Ala
20 25 30
Thr Gly Asn Tyr Arg Asn Val Phe Val Glu Thr Gly Arg Thr Glu Ala
35 40 45
Gln Val Gln Asp Lys Met Asn Thr Met Phe Asp Lys Phe Phe Lys Gly
50 55 60
Asp Thr Asn Asn Gln Arg Leu Phe Tyr Glu Thr Gly Thr Asp Glu Ala
65 70 75 80
Tyr Ile Arg Asp Thr Gly Asn Gly Asp Val Arg Ser Glu Gly Met Ser
85 90 95
Tyr Gly Met Met Val Cys Val Gln Met Asp Lys Lys Lys Glu Phe Asp
100 105 110
Met Leu Trp Lys Trp Ala Arg Asn His Met Tyr Gln Thr Ser Gly Gln
115 120 125
Phe Lys Gly Phe Phe Ala Trp Gln Cys Asn Tyr Asp Gly Gly Ile Ile
130 135 140
Asp Ser Thr Pro Ala Ser Asp Gly Asp Glu Tyr Phe Ala Met Ala Leu
145 150 155 160
Leu Phe Ala Ala Arg Arg Trp Gly Asn Gly Thr Gly Ile Tyr Asn Tyr
165 170 175
Glu Ala Glu Ala Gln Thr Ile Leu Asp Ala Met Leu His Gln Ser Asp
180 185 190
Asp Gly Val Gly Tyr Asn Met Ile Asn Lys Asn Ala Asn Gln Val Val
195 200 205
Phe Cys Pro Ser Ala Gly Asn Tyr Asp Phe Thr Asp Pro Ser Tyr His
210 215 220
Leu Pro Ala Phe Tyr Glu Leu Trp Ala Met Trp Gly Pro Glu Arg Asp
225 230 235 240
Arg Ala Thr Trp Ser Lys Val Ala Ala Thr Ser Arg Glu Phe Leu Lys
245 250 255
Lys Ser Thr His Pro Thr Thr Gly Leu Asn Pro Asp Tyr Ala Asn Phe
260 265 270
Asp Gly Ser Ala Lys Glu Val Ser Trp Ser Ser Gly His Gly Asp Phe
275 280 285
Arg Phe Asp Ala Trp Arg Val Ile Gln Asn Ser Cys Val Asp Tyr Ala
290 295 300
Trp Trp Gln Lys Asp Ser Trp Pro Ala Thr Thr Phe Ala Pro Lys Ile
305 310 315 320
Gln Ala Phe Phe Lys Asn Gln Gly Leu Ser Thr Tyr Gly Asn Gln Tyr
325 330 335
Thr Leu Ser Gly Ser Lys Leu Ser Ser Asp His Ser Pro Gly Leu Val
340 345 350
Ala Met Asn Ala Val Ser Ala Leu Ala Ser Asp Ala Thr Thr Ala Lys
355 360 365
Pro Phe Val Asp Glu Leu Trp Asn Thr Ala Val Pro Ser Gly Gln Tyr
370 375 380
Arg Tyr Tyr Asp Gly Met Leu Tyr Met Leu Gly Met Leu Asn Val Ser
385 390 395 400
Gly Asn Phe Lys Ile Trp Gly Ala Pro Thr Glu Pro Ser Val Thr Arg
405 410 415
Gly Asp Ile Asn Asp Asp Gly Thr Ile Asp Ser Val Asp Phe Ala Leu
420 425 430
Leu Lys Ser Tyr Leu Leu Gly Arg Thr Thr Thr Leu Pro Asn Met Lys
435 440 445
Ala Ala Asp Leu Asn Gly Asp Gly Val Val Asp Ala Met Asp Trp Ala
450 455 460
Val Leu Arg Gln Tyr Leu Leu Gly Ile Ile Lys Thr Leu
465 470 475
<210> 106
<211> 380
<212> PRT
<213> 寡养单胞菌属
<400> 106
Met Ala Asn Ser Ala Asp Leu His Arg Arg Arg Leu Leu Gln Ala Ala
1 5 10 15
Ala Ala Val Pro Leu Leu Gly Trp Gly Ala Ala Gln Ala Gln Pro Ala
20 25 30
Pro Ser Ala Ser Arg Trp Pro Ala Trp Gln Val Leu Leu Asp Ser Ser
35 40 45
Leu Ser Arg Asp Gly Arg Met Ile Asp Arg Ser Gln Asp Asp Gln Arg
50 55 60
Ser Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala
65 70 75 80
Asn Asp Gln Thr Leu Phe Asp Arg Ile Leu Ala Trp Thr Gln Asp Asn
85 90 95
Leu Ala Gly Gly Asp Met Arg Gln His Leu Pro Ala Trp Leu Trp Gly
100 105 110
Arg Asp Ala Lys Gly Gly Trp Gln Val Leu Asp Ser Asn Pro Ala Ser
115 120 125
Asp Ser Asp Leu Trp Leu Ala Tyr Ala Leu Leu Glu Gly Ala Arg Leu
130 135 140
Trp Arg Arg Pro Ala Leu Gln Ala Ile Ala Glu Gly Met Leu Gln Gln
145 150 155 160
Val Arg Glu Arg Glu Ile Val Gln Leu Pro Gly Leu Gly Pro Met Leu
165 170 175
Leu Pro Gly Pro Gln Gly Phe Val Glu Gly Asp Ala Thr Arg Ile Asn
180 185 190
Pro Ser Tyr Leu Pro Leu Pro Leu Leu Arg Arg Phe Ala Ser Val Asp
195 200 205
Arg Lys Gly Pro Trp Pro Ala Leu Ala Ala Asn Thr Val Arg Met Leu
210 215 220
Gln Gln Ser Ser Pro His Gly Phe Ala Pro Asp Trp Thr Ala Trp Gln
225 230 235 240
Ala Gly Gly Phe Val Ala Asp Pro Val Lys Gly Ala Val Gly Ser Tyr
245 250 255
Asp Ala Ile Arg Cys Tyr Ala Trp Ala Gly Met Thr Ala Pro Arg Asp
260 265 270
Pro Leu Phe Arg Pro Gln Leu Ala Ala Leu Ser Gly Pro Leu Gln Arg
275 280 285
Leu Arg Ser Gly Ala Ala Met Trp Glu Lys Ile Asp Thr Arg Ser Gly
290 295 300
Gln Gly Gln Gly Glu Gly Asn Tyr Gly Phe Arg Ala Ala Leu Leu Pro
305 310 315 320
Tyr Leu Leu Ala Gln Gly Glu Asn Glu Arg Ala Arg Ala Leu Gln Ala
325 330 335
Ser Leu Pro Ser Ala Glu Gln Gln Arg Ala Asp Ala Pro Ala Tyr Tyr
340 345 350
Ser Gln Met Leu Ser Leu Phe Gly Leu Gly Trp Ala Glu Gly Arg Trp
355 360 365
Arg Phe Asn Ala Asp Gly Arg Leu Gln Pro Arg Trp
370 375 380
<210> 107
<211> 356
<212> PRT
<213> 类球红细菌
<400> 107
Met Arg Arg Arg Thr Ile Leu Thr Ser Ala Ala Ala Ala Leu Met Leu
1 5 10 15
Ala Pro Ala Gly Arg Leu Leu Ala Gln Ser Gly Gly Glu Ala Leu Pro
20 25 30
Ala Asp His Pro Leu Gln Ala Ala Trp Arg Ser Trp Lys Asp Ala Phe
35 40 45
Leu Leu Pro Ala Gly Arg Ile Val Asp Gly Pro Gln Gln Asn Ala Ser
50 55 60
His Ser Glu Gly Gln Gly Tyr Gly Ala Thr Leu Ala Ala Ile Phe Gly
65 70 75 80
Asp Glu Glu Ala Leu Arg Arg Ile Val Asp Trp Thr Glu Ala Asn Leu
85 90 95
Ala Arg Arg Glu Asp Lys Leu Leu Ser Trp Arg Trp Leu Pro Gly Val
100 105 110
Ala Leu Ala Val Pro Asp Glu Asn Asn Ala Thr Asp Gly Asp Leu Phe
115 120 125
Tyr Ala Trp Gly Leu Ala Met Ala Ala Gln Arg Phe Gly Lys Ala Asp
130 135 140
Tyr Ala Gly Arg Ala Thr Glu Leu Ala Arg Ala Ile Ala Leu His Cys
145 150 155 160
Val Arg Pro His Pro Asp Gly Ser Glu Gln Leu Val Leu Leu Pro Gly
165 170 175
Ala Ser Gly Phe Glu Thr Pro Asp Gly Val Val Leu Asn Pro Ser Tyr
180 185 190
Tyr Met Pro Arg Ala Leu Thr Glu Leu Ala Ala Phe Ser Gly Gln Asp
195 200 205
Arg Leu Ala Arg Cys Ala Arg Asp Gly Ala Asp Trp Ile Ala Ser Leu
210 215 220
Gly Leu Pro Pro Asp Trp Ala Leu Val Thr Pro Phe Gly Thr Gln Pro
225 230 235 240
Ala Pro Gly Leu Ser His Asn Ser Gly Tyr Asp Ala Leu Arg Val Pro
245 250 255
Leu Phe Leu Leu Trp Ser Gly Leu Thr Ala Asn Pro Ala Leu Arg Arg
260 265 270
Ala Val Glu Ala Ala Gly Asp Ala Ala Ala Gly Asp Thr Pro Val Thr
275 280 285
Phe Asp Arg Asp Thr Gly Ala Val Leu Glu Arg Ser Ala Asp Pro Gly
290 295 300
Phe Arg Ala Val Leu Ala Leu Gly Asp Cys Ala Leu Ser Gly Arg Pro
305 310 315 320
Gly Ala Ala Ile Pro Pro Phe Asp Ala Arg Gln Pro Tyr Tyr Pro Ala
325 330 335
Thr Leu His Leu Met Ala Leu Val Ala Gln Val Glu Gly Phe Ser Ala
340 345 350
Cys Val Pro Ile
355
<210> 108
<211> 384
<212> PRT
<213> 假小链双歧杆菌
<400> 108
Met Met Arg Lys Asp Glu Thr Met Gly Ala Gly Ala Met Gly His Met
1 5 10 15
Glu Thr Ser Met Phe Glu Gln Cys Gly Tyr Gly Arg Glu Ala Ile Gln
20 25 30
Ala Arg Leu Glu Arg Asn Trp Thr Glu Met Phe Glu Gly Pro Asp Lys
35 40 45
Ile Tyr Trp Glu Asn Asp Glu Gly Leu Gly Tyr Val Met Asp Thr Gly
50 55 60
Asn His Asp Val Arg Thr Glu Gly Met Ser Tyr Ala Met Met Ile Ala
65 70 75 80
Val Gln Tyr Asp Arg Lys Asp Val Phe Asp Lys Leu Trp Gly Trp Val
85 90 95
Met Lys Tyr Met Phe Met Thr Glu Gly Leu His Lys Gly Tyr Phe Ala
100 105 110
Trp Ser Val Asp Leu Ser Gly Val Pro Asn Ala Asp Gly Pro Ala Pro
115 120 125
Asp Gly Glu Glu Tyr Phe Ala Met Ala Leu Phe Leu Ala Ser Gly Arg
130 135 140
Trp Gly Asp Gly Glu Gly Val Tyr Glu Tyr Ser Arg His Ala Arg Ser
145 150 155 160
Ile Leu His Thr Cys Val His Arg Gly Glu Asp Gly Glu Gly Tyr Pro
165 170 175
Met Trp Asn Pro Glu Asn His Leu Ile Lys Phe Ile Pro Glu Thr Glu
180 185 190
Trp Thr Asp Pro Ser Tyr His Leu Pro His Phe Tyr Glu Val Phe Ala
195 200 205
Gln Arg Ala Asp Glu Ala Asp Arg Pro Phe Trp Arg Glu Ala Ala Ala
210 215 220
Ala Ser Arg Arg Tyr Leu Val Thr Ala Cys His Pro Glu Thr Gly Met
225 230 235 240
Asn Pro Glu Tyr Ser Asn Tyr Asp Gly Thr Pro His Val Asp Glu Arg
245 250 255
Asp His Trp His Phe Tyr Ser Asp Ala Tyr Arg Thr Ala Gly Asn Ile
260 265 270
Gly Leu Asp Cys Leu Trp Asn Gly Val Val Pro Glu Leu Cys Asp Ala
275 280 285
Asn Ala Arg Leu Gln Arg Phe Phe Leu Glu His Asp Arg Thr Cys Val
290 295 300
Tyr Ala Val Asp Gly Thr Pro Val Asp Glu Thr Val Leu His Pro Val
305 310 315 320
Gly Phe Ile Ala Ala Thr Ala Glu Gly Ser Leu Ala Ala Met His Ser
325 330 335
Gln Glu Pro Asp Ala Leu Asp Asn Ala Ile Arg Trp Val Arg Leu Leu
340 345 350
Trp Asp Thr Pro Ile Arg Thr Gly Thr Arg Arg Tyr Tyr Asp Asn Phe
355 360 365
Leu Tyr Ala Phe Ala Phe Leu Ala Leu Ala Gly Glu Tyr Arg Val Trp
370 375 380
<210> 109
<211> 388
<212> PRT
<213> Subdoligranulum variabile
<400> 109
Met Ile Met Glu Gly Ala Tyr Tyr Asn Gly Gln Tyr Leu Asn Arg Leu
1 5 10 15
Glu Arg Leu Gly Tyr Ser Gln Ala Glu Ile Asp Asn Lys Leu Glu Ser
20 25 30
Thr Phe Glu Glu Met Phe Tyr Gly Pro Gln Gly Val Arg Leu Cys His
35 40 45
Phe Ala Gly Pro Asp Met Met Tyr Ile Glu Asp Thr Gly Asn His Asp
50 55 60
Val Arg Thr Glu Gly Met Ser Tyr Ala Met Met Phe Cys Val Gln Met
65 70 75 80
Asn Arg Gln Lys Glu Phe Asp Cys Leu Trp Arg Trp Val Val Thr His
85 90 95
Met Tyr Leu Thr Glu Gly Glu Asn Ala Gly Tyr Phe Ala Trp Ser Cys
100 105 110
His Thr Asp Gly Ser Lys Asn Ser Asp Gly Pro Ala Pro Asp Gly Glu
115 120 125
Glu Phe Phe Ala Met Ser Leu Phe Phe Ala Ala Asn Arg Trp Gly Ser
130 135 140
Gly Glu Gly Val Leu Asp Tyr Ala Ser Trp Ala Arg Arg Ile Leu His
145 150 155 160
Thr Cys Val His Lys Gly Glu Glu Ala Gly Ser Gly Phe Pro Met Trp
165 170 175
Asn Pro Glu Asn His Leu Ile Lys Phe Val Pro Asn Cys Glu Phe Thr
180 185 190
Asp Pro Ser Tyr His Leu Pro His Phe Tyr Glu Leu Phe Ala Leu Trp
195 200 205
Ser Asp Glu Cys Asp Arg Pro Phe Trp His Ala Ala Ala Ala Ala Ser
210 215 220
Arg Gln Tyr Leu Arg Lys Ala Cys His Ala Arg Thr Gly Leu Ser Ala
225 230 235 240
Glu Tyr Ala Glu Tyr Asp Gly Arg Pro His Arg Gly Asp Gln Pro Asp
245 250 255
Arg His Asp Trp Phe Phe Ser Asp Ala Tyr Arg Thr Leu Gly Asn Ile
260 265 270
Ala Leu Asp Ala Gln Trp Phe Gly Asp Lys Asp Gly Trp Ala Gln Glu
275 280 285
Thr Ala Ala His Ile Gln Gln Phe Phe Glu Glu Lys Glu His Gly Gln
290 295 300
Thr Asn Gly Ile Tyr Leu Ile Asp Gly Thr Pro Val Glu Gly Asn Ala
305 310 315 320
Leu His Pro Val Gly Leu Leu Ala Thr Ile Ala Gln Gly Ser Leu Ile
325 330 335
Leu Asp Asp Ala Cys Ala Asp Glu Trp Leu Arg Arg Phe Phe Ser Thr
340 345 350
Pro Leu Arg Ser Gly Pro Arg Arg Tyr Tyr Asp Asn Cys Leu Tyr Leu
355 360 365
Phe Ala Leu Leu Ala Leu Ser Gly His Tyr Arg Ile Trp Phe Pro Gln
370 375 380
Glu Ala Lys Val
385
<210> 110
<211> 350
<212> PRT
<213> 盐单胞菌属
<400> 110
Met Asn Gly Lys Ser Val Lys Ala Ala Val Ile Tyr Leu Leu Gly Gly
1 5 10 15
Leu Leu Val Leu Leu Gly Leu Ala Ser Met Pro Ala Ser Ala Asn Ala
20 25 30
Asn Phe Ala Val Gly Asp Pro Gly Trp Glu Ala Tyr Lys Lys Arg Phe
35 40 45
Leu Leu Pro Glu Gly Arg Ile Val Asp Thr Ala Asn Asn Asn Ile Ser
50 55 60
His Thr Glu Gly Gln Gly Trp Gly Met Phe Leu Ala Val Gln Phe Asn
65 70 75 80
Asp Arg Gln Ala Phe Asp Arg Ile Trp Gln Trp Thr Glu Ala His Leu
85 90 95
Ala Arg Gln Asp Ile Ala Leu Tyr Ala Trp Arg Tyr Asp Pro Asn Ala
100 105 110
Gln Pro Pro Val Ala Asp Asn Asn Asn Ala Thr Asp Gly Asp Leu Phe
115 120 125
Ala Ala Trp Ser Leu Gln Leu Ala Ala Asp Arg Trp Gly Asp Glu Arg
130 135 140
Tyr Ala Gln Arg Ser Glu Ala Ile Arg Gly Ala Ile Arg Asp His Leu
145 150 155 160
Ile Ala Asp Val Gly Gly Tyr Gln Val Leu Leu Pro Gly Leu Asp Gly
165 170 175
Phe Arg His Lys Thr Phe Thr Asp Ile Asn Leu Ser Tyr Trp Val Ile
180 185 190
Pro Ala Leu Arg Asp Phe Ala Ala Arg His Pro Asp Glu Pro Trp Leu
195 200 205
Ala Val Ile Asp Ser Gly Arg Gln Leu Leu Glu Arg Ala Gln Phe Gly
210 215 220
Ser Ser Gln Leu Pro Ala Asp Trp Leu Arg Leu Gln Thr Ser Gly Glu
225 230 235 240
Leu Arg Pro Ala Glu Gly Trp Pro Pro Arg Phe Gly Phe Glu Asn Ile
245 250 255
Arg Thr Pro Leu Tyr Phe Thr Trp Gly Gly Leu Arg Asp Ile Asp Thr
260 265 270
Leu Glu Asp Ile Ala Arg Phe Trp Asp Gln Ser Ala Pro Pro Ala Trp
275 280 285
Val Asp Val Glu Ser Gly Asp Thr Ala Glu Tyr Pro Ile Ser Gln Gly
290 295 300
Gly Lys Ala Ile Ser Ala Leu Leu Ser Gly Arg Pro Trp Ala Ile Asp
305 310 315 320
Ile Thr Pro Ala Ala Gly Glu Asn Tyr Tyr Ser Ala Thr Leu Leu Leu
325 330 335
Leu Thr Arg Val Ala Ala Ala Gln Ile Ser Leu Ser Ala Gly
340 345 350
<210> 111
<211> 390
<212> PRT
<213> 昏暗地嗜皮菌
<400> 111
Met Ser Pro Arg Gly Ile Thr Val Gly Ala Leu Gly Val Leu Ala Leu
1 5 10 15
Leu Val Val Val Leu Ala Ala Val Val Gly Thr Arg Gly Gly Gly Thr
20 25 30
Thr Gly Ala Pro Leu Pro Ser Pro Thr Gly Pro Ser Thr Val Ala Pro
35 40 45
Ala Pro Pro Ala Glu Leu Arg Thr Tyr Thr Ala Ala Glu Ala Gly Arg
50 55 60
Ala Phe Leu Asp Gly Tyr Val Asp Glu Asp Gly Gln Val Val Arg Pro
65 70 75 80
Asp Gln Gly Gly Asp Thr Val Ser Glu Gly Gln Ala Tyr Ala Met Leu
85 90 95
Val Ala Val Gly Leu Gly Asp Ala Lys Thr Phe Ala Arg Ala Trp Asn
100 105 110
Trp Thr Arg Glu Asn Leu Gln Arg Pro Asp Gly Leu Leu Ser Trp Arg
115 120 125
Trp Glu Glu Gly Gly Val Val Asp Ala Ser Ser Ala Ser Asp Ala Asp
130 135 140
Leu Asp Ala Ala Arg Ala Leu Val Leu Ala Gly Glu Gln Phe Gly Arg
145 150 155 160
Pro Glu Tyr Thr Ala Ala Gly Leu Asp Leu Gly Arg Ala Val Leu Asp
165 170 175
Leu Glu Thr Val Pro Thr Ala Ala Gly Arg Ile Leu Thr Ala Gly Gln
180 185 190
Trp Ala Thr Thr Glu Pro Tyr Ala Tyr Asn Pro Ser Tyr Ala Ser Pro
195 200 205
Gly Ala Thr Ala Val Leu Ala Ala Ala Ser Gly Asp Pro Arg Trp Thr
210 215 220
Glu Leu Ala Glu Gly Ser Arg Ala Val Thr Ala Ala Leu Leu Ser Gln
225 230 235 240
Ala Pro Leu Pro Pro Asp Trp Ala Gln Val Arg Ala Asp Gly Thr Val
245 250 255
Glu Ala Met Pro Gly Ala Met Gly Arg Gly Gln Ser Val Arg Tyr Gly
260 265 270
Tyr Asp Ala Thr Arg Thr Pro Ile Arg Phe Ala Glu Ser Cys Asp Pro
275 280 285
Ala Asp Arg Ala Leu Ala Ala Ala Val Val Glu Pro Leu Asp Arg Gly
290 295 300
Gly Asp Ser Ala Glu Leu Asp Leu Gly Gly Ser Pro Val Ala Pro Gly
305 310 315 320
Glu Ser Val Val Ala Ala Ala Gly Gln Ala Ala Ala Val Ala Ala Ala
325 330 335
Gly Asp Ala Gly Arg Ala Val Glu Glu Leu Val Asp Ala Asp His Leu
340 345 350
Ala Gln Ser Ala Pro Ser Tyr Tyr Gly Ala Ala Trp Ala Ala Leu Gly
355 360 365
Arg Phe Met Leu Thr Asp Asp Val Leu Gly Gly Cys Pro Pro Val Pro
370 375 380
Ala Thr Ser Gly Thr Ala
385 390
<210> 112
<211> 372
<212> PRT
<213> 加氏乳杆菌
<400> 112
Met Lys Val Lys Asn Leu Ile Leu Ile Leu Gly Thr Ile Ile Ala Tyr
1 5 10 15
Leu Gly Ile Ile Gly Tyr Val Arg Met Ser Asn Asp Arg Thr Leu Glu
20 25 30
Lys Arg Tyr Tyr His Glu Trp Arg Glu Asp Tyr Ile Lys Asn Lys Asn
35 40 45
Gln Asn Glu Gln Tyr Val Asn Ala Ala Gly Gly Lys Gly Pro Ser Phe
50 55 60
Ala Leu Ser Glu Ala Gln Gly Tyr Gly Met Leu Leu Ala Ala Lys Ala
65 70 75 80
Gly Glu Lys His Leu Gly Ser Arg Ile Asp Phe Gln Lys Leu Asp Asn
85 90 95
Tyr Tyr Leu Ala His Arg Leu Ile Asn Ser Asn Leu Met Ser Trp Arg
100 105 110
Gln Lys Asp Arg Lys Asn Ile Trp Arg Asp Asn Pro Val Ser Ala Ser
115 120 125
Asp Gly Asp Ile Met Ile Ala Gln Ala Leu Leu His Ala Asn Arg Val
130 135 140
Trp Pro Gly His Gly Tyr Lys Ser Gln Ala Val Asn Leu Ile Asn Asp
145 150 155 160
Ile Lys Arg Leu Glu Ile Asn Gln Lys Ala Lys Met Val Thr Val Gly
165 170 175
Asn Trp Ala Asn Lys Asp Ser Arg Phe Tyr Asn Val Leu Arg Thr Ser
180 185 190
Asp Val Met Pro Lys Ala Phe Glu Glu Phe Tyr Gln Ala Thr Gly Asp
195 200 205
Arg Glu Trp Leu Thr Ile Lys Ser Lys Met Leu Asn Tyr Leu Gln Lys
210 215 220
Leu Ser Lys Lys His Asp Thr Gly Leu Val Pro Asp Phe Ala Trp Val
225 230 235 240
Ser Asn Asn Tyr Val Lys Pro Ala Lys Ala Asn Glu Val Ala Thr Asn
245 250 255
Asp Asp Gly His Tyr Ser Ala Asn Ala Cys Arg Val Pro Met Leu Leu
260 265 270
Ala Glu Ser Asn Asp Lys Asn Ala Ala Gln Val Val Lys Lys Met Leu
275 280 285
Lys Phe Phe Lys Lys Lys Gly Thr Ser Ala Gly Phe Thr Leu Lys Gly
290 295 300
Lys Lys Leu His Tyr Tyr Gln Ser Ala Ser Phe Ser Ala Pro Ile Phe
305 310 315 320
Val Ala Ala Asn Lys Tyr Arg Asn Gln Gly Tyr Asp Thr Leu Val Glu
325 330 335
Gln Glu Lys Tyr Ile Phe Ser Arg Ser Leu Pro Lys Asp Asn Tyr Tyr
340 345 350
Asp Ala Thr Leu Thr Thr Leu Ala Ala Leu Asn Thr Asn Glu Leu Thr
355 360 365
Gly Leu Glu Lys
370
<210> 113
<211> 337
<212> PRT
<213> 维涅兰德固氮菌
<400> 113
Met Ala Ala Arg Lys Ala Gly Pro Leu Gly Ala Leu Ile Ala Leu Leu
1 5 10 15
Leu Phe Cys Leu Pro Ala Ser Ala Gly Glu Asp Glu Tyr Trp Arg Leu
20 25 30
Tyr Gln Thr Arg Tyr Ile Asp Gly Gly Arg Val Leu Asp Thr Gly Asn
35 40 45
Lys Gly Val Ser His Ser Glu Gly Gln Gly Trp Gly Met Leu Leu Ala
50 55 60
Glu Ala Asn Gly Asp Arg Arg Thr Phe Asp Arg Leu Trp Ser Trp Thr
65 70 75 80
Arg Arg His Leu Gln Arg Arg Asp Leu Ala Leu Phe Ser Trp Arg Tyr
85 90 95
Asp Pro Ala Ala Ser Pro Pro Val Gln Asp Leu Asn Asn Ala Thr Asp
100 105 110
Gly Asp Leu Leu Ile Ala Trp Ala Leu Leu Arg Ala Ala Gln Arg Trp
115 120 125
Asp Ala Ala Asp Tyr Arg Asn Ala Ser Ala Lys Ile Arg Gln Ala Ile
130 135 140
Ala Gly Arg Leu Ile Lys Glu His Ala Gly Leu Thr Val Leu Leu Pro
145 150 155 160
Gly Leu Gln Gly Phe Glu Gln Asn Gly Gln Leu Ile Leu Asn Pro Ser
165 170 175
Tyr Leu Val Met Pro Ala Ile Arg Ala Phe Ala Arg Ala Asp Pro Gly
180 185 190
Gly Gly Trp Gln Arg Leu Leu Asp Asp Ser Arg Ser Leu Leu Asp Arg
195 200 205
Ala Arg Phe Gly Thr Tyr Arg Leu Pro Pro Asp Trp Leu Ala Leu Gly
210 215 220
Ala Asp Gly Thr Leu Glu Pro Ala Ala Ala Trp Pro Ala Arg Phe Gly
225 230 235 240
Phe Asp Ala Val Arg Val Pro Leu Tyr Leu Ala Trp Asp Gly Glu Pro
245 250 255
Leu Ser Ser Pro Arg Leu Ala Pro Phe Ala Asp Phe Trp Ser Cys Cys
260 265 270
Glu Pro Leu His Ala Trp Val Asp Leu Asn Ser Gly Lys Leu Ser Pro
275 280 285
Tyr Pro Gly Ser Val Gly Val Lys Ala Ile Ala Ala Leu Leu Arg Gly
290 295 300
Glu Glu Pro Val Ala Thr Pro Glu Gln Leu Ala Lys Glu Asp Tyr Tyr
305 310 315 320
Ser Met Ser Leu Leu Met Leu Ala Arg Leu Ala Arg Lys Glu Gly Ala
325 330 335
Pro
<210> 114
<211> 320
<212> PRT
<213> Sulfurihydrogenibium azorense
<400> 114
Met Lys Arg Leu Leu Ile Ile Phe Ser Val Leu Gly Phe Leu Gly Phe
1 5 10 15
Ser Asn Gly Gln Glu Ile Leu Thr Trp Gln Asn Tyr Lys Asn Lys Tyr
20 25 30
Ile Pro Asn Gly Ser Tyr Val Ile Asp Pro Tyr Asn Glu Asn Arg Val
35 40 45
Thr Ser Glu Ser Gln Gly Tyr Gly Met Ile Leu Ala Ile Leu Asn Asp
50 55 60
Asp Lys Thr Thr Phe Asp Asn Leu Trp Gln Trp Thr Arg Lys Asn Leu
65 70 75 80
Gln Arg Glu Asp Tyr Leu Phe Ser Trp Leu Trp Asn Asp Glu Val Lys
85 90 95
Asp Lys Asn Asn Ala Thr Asp Gly Asp Phe Leu Ile Ala Tyr Ala Leu
100 105 110
Leu Lys Ala Tyr Glu Lys Trp Gly Asp Lys Ala Tyr Lys Asp Glu Gly
115 120 125
Glu Lys Ile Phe Asn Ser Leu Lys Asn Leu Ile Val Ile Val Lys Asp
130 135 140
Asn Lys Leu Lys Asp Asn Tyr Leu Leu Leu Pro Ala Thr Tyr Gly Phe
145 150 155 160
Ser Asn Glu Lys Tyr Asp Ile Val Ile Phe Pro Ser Tyr Tyr Ile Thr
165 170 175
Phe Ile Leu Lys Glu Leu Ser Tyr Lys Asp Asn Leu Trp Lys Gly Val
180 185 190
Tyr Asn Tyr Thr Lys Asn Ile Leu Phe Lys Thr Ile Leu Ser Thr Asn
195 200 205
Leu Lys Phe Asn Leu Ile Glu Lys Lys Leu Ile Pro Ile Ser Pro Val
210 215 220
Asn Leu Asp Val Tyr Arg Val Ile Pro Tyr Thr Tyr Met Ala Lys Glu
225 230 235 240
Ser Leu Glu Asp Leu Lys Thr Ser Phe Ser Glu Val Asp Ser Phe Phe
245 250 255
Lys Ala Lys Gly Tyr Ile Pro Phe Asn Tyr Asn Leu Gly Ser Leu Gln
260 265 270
Gln Glu Val Ser Glu Ser Pro Phe Cys Val Tyr Arg Phe Phe Tyr Leu
275 280 285
Leu Tyr Asn Asp Glu Lys Tyr Leu Glu Arg Tyr Lys Val Leu Lys Asn
290 295 300
Asn Asp Lys Asn Asn Tyr Phe Cys Asp Thr Phe Glu Leu Phe Leu Asp
305 310 315 320
<210> 115
<211> 388
<212> PRT
<213> 荚膜酸杆菌
<400> 115
Met Met Arg Met Arg Met Lys Leu Leu Arg His Val Leu Ser Val Ala
1 5 10 15
Ser Leu Ser Ala Leu Ile Phe Ala Thr Gly Cys Arg Ala Gln Ser Trp
20 25 30
Pro Leu Trp Gln Ala Tyr Gln Gly Lys Phe Leu Asn Ser Asp Gly Arg
35 40 45
Val Val Asp Tyr Asn Ala Gln Ala Arg Thr Thr Ser Glu Gly Gln Ser
50 55 60
Tyr Ala Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Pro Ala Phe Asp
65 70 75 80
Lys Val Leu Ala Trp Thr Gln Asn Asn Leu Ala Gln Gly Asp Leu Thr
85 90 95
Ala His Leu Pro Ala Trp Glu Trp Gly Lys Ala Lys Asp Gly Gln Trp
100 105 110
Lys Thr Ile Asp Pro Asn Pro Ala Ser Asp Ala Asp Leu Trp Ile Ser
115 120 125
Tyr Thr Leu Leu Gln Ala Gly Arg Leu Trp His Asp Pro His Tyr Thr
130 135 140
Ala Leu Gly Thr Val Met Ala Lys Arg Ile Ala Asn Glu Glu Val Ala
145 150 155 160
Asn Leu Pro Gly Leu Gly Ser Met Leu Leu Pro Gly Pro Thr Gly Phe
165 170 175
His Pro Asn Ala Ala Thr Trp Leu Leu Asn Pro Ser Tyr Met Pro Leu
180 185 190
Pro Val Val Glu Gly Met Ala His Ala Asp Pro Ser Gly Pro Trp Ala
195 200 205
Gly Met Ala Ala Ala Val Pro Asp Leu Val Lys Gly Ala Ser Pro Ala
210 215 220
Gly Phe Val Met Asp Trp Val Ser Tyr Ser Gln Ala Gly Gly Phe Gln
225 230 235 240
Pro Ala Val Leu Pro Thr Ala Pro Lys Gly Thr Val Pro Met Gly Ser
245 250 255
Tyr Asp Ala Ile Arg Val Tyr Leu Trp Thr Gly Met Ala Asn Pro Ala
260 265 270
Thr Pro Gly Ala Lys Leu Thr Leu Arg Ala Leu Tyr Gly Met Ala Ala
275 280 285
Tyr Leu Lys Ser His Thr Leu Pro Pro Glu Lys Val Asp Pro Gln Gly
290 295 300
Lys Val Leu Ser Thr Ser Ala Pro Val Gly Phe Ser Ala Ala Val Glu
305 310 315 320
Pro Phe Leu Ser Ala Leu Gly Asp Lys Gly Asp Leu Asn Thr Gln Gln
325 330 335
Ala Arg Leu Asp Ala Met Val Asn Pro Asn Thr Lys Leu Tyr Gly Glu
340 345 350
Pro Pro Ala Tyr Phe Asp Gln Asn Leu Ala Met Phe Gly Glu Gly Trp
355 360 365
Gln Gln His Arg Phe Gly Phe Ala Ser Asp Gly Asp Leu Trp Val Lys
370 375 380
Trp Lys Gly Arg
385
<210> 116
<211> 381
<212> PRT
<213> 詹氏乳杆菌
<400> 116
Met Glu Asp Ile Met Lys Gln Lys Ile Met Trp Val Ile Leu Pro Ile
1 5 10 15
Ile Ile Ala Asn Tyr Ile Ala Thr Phe Tyr Phe Ile Arg Thr Lys Asn
20 25 30
Pro Glu Gln Ile Gln Ile Lys Thr Tyr Gln Asp Trp Lys Lys Asn Tyr
35 40 45
Leu Val Thr Lys Asn Ser Asn Gln Val Phe Val Asn Ala Gly Thr Asn
50 55 60
Lys His Pro Val Ala Leu Ser Glu Ala Gln Gly Phe Gly Leu Ile Ile
65 70 75 80
Thr Ala Lys Ala Gly Lys Arg Gly Trp Ala Ser Glu Thr Glu Phe Asp
85 90 95
Lys Leu Leu Asn Tyr Tyr Leu Ala His Gln Asp Tyr Val Gly Asp His
100 105 110
His Thr Gln Lys Val Ser Leu Met Gln Trp Lys Gln Tyr Tyr Asn His
115 120 125
His Gly Lys Trp Val Ser Glu Tyr Asn Ser Ala Thr Asp Gly Asp Leu
130 135 140
Tyr Ile Ala Thr Ala Leu Asn Thr Ala Ala Lys Val Trp Pro Gln Lys
145 150 155 160
Ala Asn Tyr Tyr His Ser Leu Glu Ala Lys Leu Ala Asn Asp Ile Leu
165 170 175
Arg Tyr Glu Tyr Asn Pro Gln Thr Gly Ala Leu Thr Thr Gly Asp Trp
180 185 190
Val Arg Leu Asp Ser Tyr Tyr Ala Asn Leu Met Arg Thr Ser Asp Val
195 200 205
Leu Pro Phe Val Phe Thr Asn Leu Ala Lys Thr Thr Gly Asn Lys Gln
210 215 220
Trp Tyr Ala Val Gln Asp Ser Met Leu Glu Lys Leu Val Lys Leu Ser
225 230 235 240
Lys Gln His Lys Ala Gly Leu Val Pro Asp Phe Ala Trp Val Gly Arg
245 250 255
Asn Tyr Ala Lys Pro Val Ala Pro Lys Thr Ile Ala Gly Lys Asn Asp
260 265 270
Gly Tyr Tyr Ala Tyr Asn Ala Cys Arg Val Pro Ile Met Leu Ala Lys
275 280 285
Ser Glu Ser Pro Lys Ala Lys Phe Val Glu Lys Arg Ile Leu His Tyr
290 295 300
Phe Ser Lys Gln Tyr Asn Val Phe Gly Gly Tyr Lys Leu Asn Gly Glu
305 310 315 320
Arg Leu Val Lys Asn Gln Ser Pro Ser Phe Ser Ala Pro Ile Phe Tyr
325 330 335
Ala Val Asn Gln Tyr Arg Gly Gln Gly Tyr Asp Asn Leu Phe Val Ser
340 345 350
Gln Lys Tyr Ile Phe Ser Lys Ala Leu Pro Lys Asn Asp Tyr Tyr Gly
355 360 365
Ala Thr Leu Thr Thr Leu Val Ala Val Glu Gly Trp Glu
370 375 380
<210> 117
<211> 432
<212> PRT
<213> 舌螺状菌
<400> 117
Met Asn Leu Ile Asn Asn Asn Leu Pro Glu Asn Arg Trp Leu Leu Pro
1 5 10 15
Val Leu Ile Gly Ile Phe Leu Ile Val Thr Pro Met Ala Val Ala Gln
20 25 30
Lys Lys Met Lys Asp Lys Pro Lys Ala Glu Ala Gln Val Gly Asn Tyr
35 40 45
Arg Asn Leu Phe Arg Glu Ala Gly Tyr Lys Gln Ala Asp Ile Asp Ala
50 55 60
Lys Leu Ala Lys Ala Tyr His Asp Val Phe Glu Gly Pro Asn Lys Val
65 70 75 80
Tyr Phe Glu Val Gly Asp Thr Met Ala Tyr Val Ser Asp Val Lys Asn
85 90 95
Lys Asp Ala Arg Thr Glu Gly Leu Ser Tyr Gly Met Met Ile Ala Val
100 105 110
Gln Leu Asp Lys Lys Asp Val Phe Asp Arg Ile Trp Arg Trp Ser Lys
115 120 125
Lys Tyr Leu Gln His Gln Ser Gly Pro Arg Glu Gly Tyr Phe Ala Trp
130 135 140
Ser Ile Asn Pro Gln Thr Met Lys Lys Asn Ser Glu Gly Ser Ala Ser
145 150 155 160
Asp Gly Glu Leu Tyr Tyr Ile Thr Ser Leu Leu Leu Ala Ala Asn Lys
165 170 175
Trp Gly Asn Asn Thr Gly Ile Asn Tyr Tyr Gly Glu Ala Arg Arg Ile
180 185 190
Leu Asp Ala Ile Trp Lys Lys Asp Gly Thr Gly Asn Ile Tyr Asn Leu
195 200 205
Ile Asn Thr Asp Thr Lys Gln Ile Ser Phe Val Pro Glu Gly Gly Met
210 215 220
Tyr Asn Trp Thr Asp Pro Ser Tyr His Leu Pro Ala Phe Tyr Glu Val
225 230 235 240
Trp Gly Thr Tyr Ala Lys Asp Gly His Glu Gln Phe Tyr Arg Glu Cys
245 250 255
Ala Asp Thr Ser Arg Ala Phe Leu His Arg Ala Cys His Pro Val Thr
260 265 270
Gly Leu Asn Ala Asp Tyr Thr Glu Phe Ser Gly Lys Pro His Asp Thr
275 280 285
Arg Trp Ser Pro Ser Ala Phe Arg Tyr Asp Ser Trp Arg Val Pro Met
290 295 300
Asn Ile Ala Met Asp Tyr Thr Trp Ser Gly Lys Asp Lys Ala Trp Gln
305 310 315 320
Glu Asp Tyr Ala Lys Arg Phe Gln Gly Phe Leu Arg Ser Lys Gly Met
325 330 335
Asp Thr Tyr Asp Asp Gln Phe Asn Leu Asp Gly Ser Arg Pro Glu Phe
340 345 350
Ile Leu Gln Ala Gly Pro Val Lys Lys Leu Arg His Ser Leu Gly Leu
355 360 365
Val Ser Thr Ser Ala Thr Leu Ser Leu Val Asn Lys Glu Pro Asn Ser
370 375 380
Lys Asp Phe Val Arg Ala Val Trp Asn Ala Lys Leu Glu Pro Phe Asp
385 390 395 400
Asp Gly Tyr Phe Asp Pro Tyr Tyr Asp Gly Leu Leu Tyr Val Phe Ser
405 410 415
Leu Met His Leu Ser Gly Lys Tyr Arg Ile Ile Thr Pro Gln Ala Arg
420 425 430
<210> 118
<211> 373
<212> PRT
<213> 鲶鱼爱德华氏菌
<400> 118
Met Lys Glu Ser Val Tyr Arg Thr Leu Met Ala Ala Ala Leu Leu Cys
1 5 10 15
Val Ser Val Ala Thr Val Gly Ala Pro Cys Glu Trp His Glu Trp Glu
20 25 30
Gln Phe Arg Gln Gly Tyr Ile Ser Pro Asp Gly Arg Val Ile Asp Pro
35 40 45
Ser Ser Pro Arg Gln Val Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu
50 55 60
Phe Phe Ala Leu Val Asn Asn Asp Arg Ala Leu Phe Gly Lys Leu Leu
65 70 75 80
Ala Trp Thr Gln Asn Asn Leu Ser Gln Gly Ser Leu Ser Gln Arg Leu
85 90 95
Pro Ala Trp Lys Trp Gly Arg His Asp Asp Gly Arg Trp Arg Ile Leu
100 105 110
Asp Asp Asn Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Thr Leu
115 120 125
Leu Glu Ala Gly Arg Leu Trp Gln Asp Ser Asp Tyr Gln His Leu Ser
130 135 140
Ile Gln Leu Leu Ala Arg Ile Ala Arg Glu Glu Val Val Ser Leu Pro
145 150 155 160
Gly Phe Gly Met Met Leu Leu Pro Gly Arg Gln Gly Phe Arg Ser Gly
165 170 175
Ala Leu Trp Thr Ile Asn Pro Ser Tyr Leu Pro Pro Gln Ile Leu Ala
180 185 190
Arg Ile Ala Pro Leu His Gly Pro Trp Asn Arg Leu Gln Tyr Ser Ala
195 200 205
Leu Arg Met Leu Glu Ala Ser Ser Pro His Gly Ile Val Pro Asp Trp
210 215 220
Thr Asn Trp Gln Arg Asn Lys Gly Trp Leu Pro Thr Pro Gly Arg Ala
225 230 235 240
Tyr Ile Ser Gly Tyr Asp Ala Ile Arg Ala Tyr Leu Trp Ala Gly Met
245 250 255
Leu Ala Asp Gly Ala Pro Ala Lys Ser Ala Leu Val Lys His Trp Gln
260 265 270
Pro Met Val Ala Leu Ser Glu Arg Leu Gly Tyr Ile Pro Glu Lys Val
275 280 285
Gly Val Leu Gly Val Glu Pro Gln Gly Glu Gly Asn Val Gly Phe Ser
290 295 300
Ala Ala Met Leu Pro Phe Leu Asp Ala Ser Asn Ser Pro Leu Leu Ala
305 310 315 320
Ala Gln Arg Gln Arg Val Lys Asp Asn Leu Pro Gly Asn Asn Asn Tyr
325 330 335
Tyr Ser Ser Val Leu Thr Leu Phe Gly Leu Gly Trp Asp Gln Arg Arg
340 345 350
Tyr Arg Phe Asn Leu Gln Gly Glu Leu Val Pro Asn Trp Gly Ser Val
355 360 365
Cys Asp Val Ser Asp
370
<210> 119
<211> 436
<212> PRT
<213> Teredinibacter turnerae
<400> 119
Met Phe Pro Arg Leu Ser Pro Ser Arg Phe Arg Gln Val Thr Leu Thr
1 5 10 15
Leu Leu Thr Leu Gly Leu Val Ser Leu Thr Gly Cys Ala Gly Asn Ser
20 25 30
Lys Pro Asp Ala Asp Thr Ser Thr Ala Gly Ala Val Ala Thr Gly Glu
35 40 45
Tyr Arg Asn Leu Phe Ala Glu Ile Gly Lys Ser Glu Ile Asp Ile Gln
50 55 60
Arg Lys Ile Asp Glu Ala Phe Gln His Leu Phe Tyr Gly Asp Ala Lys
65 70 75 80
Asp Ala Ala Val Tyr Tyr Gln Ala Gly Gly Asn Glu Asn Gly Pro Leu
85 90 95
Ala Tyr Val Tyr Asp Val Asn Ser Asn Asp Val Arg Ser Glu Gly Met
100 105 110
Ser Tyr Gly Met Met Ile Thr Val Gln Met Asp Lys Lys Ala Glu Phe
115 120 125
Asp Ala Ile Trp Asn Trp Ala Lys Thr Tyr Met Tyr Gln Asp Ser Pro
130 135 140
Thr His Pro Ala Phe Gly Tyr Phe Ala Trp Ser Met Arg Arg Asp Gly
145 150 155 160
Val Ala Asn Asp Asp Met Pro Ala Pro Asp Gly Glu Glu Tyr Phe Val
165 170 175
Thr Ala Leu Tyr Phe Ala Ala Ala Arg Trp Gly Asn Gly Glu Gly Ile
180 185 190
Phe Asn Tyr Gln Gln Glu Ala Asp Thr Ile Leu Ser Arg Met Arg His
195 200 205
Arg Gln Val Ile Thr Gly Pro Thr Asn Arg Gly Val Met Thr Ala Thr
210 215 220
Asn Leu Phe His Pro Glu Glu Ala Gln Val Arg Phe Thr Pro Asp Ile
225 230 235 240
Asn Asn Ala Asp His Thr Asp Ala Ser Tyr His Leu Pro Ser Phe Tyr
245 250 255
Glu Ile Trp Ala Arg Val Ala Pro Gln Glu Asp Arg Ala Phe Trp Ala
260 265 270
Lys Ala Ala Asp Val Ser Arg Asp Tyr Phe Ala Lys Ala Ala His Pro
275 280 285
Val Thr Ala Leu Thr Pro Asp Tyr Gly Asn Phe Asp Gly Thr Pro Trp
290 295 300
Ala Ala Ser Trp Arg Pro Glu Ser Val Asp Phe Arg Tyr Asp Ala Trp
305 310 315 320
Arg Ser Val Met Asn Trp Ser Met Asp Tyr Ala Trp Trp Gly Lys Asp
325 330 335
Ser Gly Ala Pro Ala Arg Ser Asp Lys Leu Leu Ala Phe Phe Glu Thr
340 345 350
Gln Glu Gly Lys Met Asn His Leu Tyr Ser Leu Asp Gly Lys Pro Leu
355 360 365
Gly Gly Gly Pro Thr Leu Gly Leu Ile Ser Met Asn Ala Thr Ala Ala
370 375 380
Met Ala Ala Thr Asp Pro Arg Trp His Asn Phe Val Glu Lys Leu Trp
385 390 395 400
Gln Gln Gln Pro Pro Thr Gly Gln Tyr Arg Tyr Tyr Asp Gly Val Leu
405 410 415
Tyr Leu Met Ala Leu Leu His Cys Ala Gly Glu Tyr Lys Ala Trp Ile
420 425 430
Pro Asp Gly Glu
435
<210> 120
<211> 325
<212> PRT
<213> Aquifex aeolicus
<400> 120
Met Lys Phe Phe Thr Val Leu Leu Phe Phe Leu Ser Phe Val Phe Ser
1 5 10 15
Ala Ser Ile Asp Val Trp Lys Leu Trp Glu His Tyr Lys Lys Thr Phe
20 25 30
Ile Ser Lys Glu Gly Tyr Val Val Asp Pro Tyr Asn Asn Tyr Arg Val
35 40 45
Thr Ser Glu Ala Gln Gly Tyr Thr Leu Leu Ile Ser Ala Leu Ile Gly
50 55 60
Asp Lys Glu Thr Phe Tyr Arg Val Trp Asn Trp Thr Lys Glu Asn Leu
65 70 75 80
Lys Arg Lys Asp Asn Leu Phe Ser Trp Leu Trp Ile Asn Gly His Val
85 90 95
Val Asp Arg Asn Asn Ala Thr Asp Ala Asp Leu Phe Ile Ala Tyr Ala
100 105 110
Leu Leu Ile Ala Ser Gln Lys Trp Lys Asp Tyr Thr Leu Leu Ser Glu
115 120 125
Ala Lys Arg Ile Lys Asp Ser Val Lys Glu Leu Val Val Pro Val Cys
130 135 140
Asn Gly Arg Arg Asp Tyr Leu Phe Ile Pro Ala Lys Glu Gly Tyr Ile
145 150 155 160
Lys Asn Asn Ile Val Ser Leu Asn Val Val Tyr Tyr Val Pro Phe Ile
165 170 175
Phe Arg Lys Phe Tyr Glu Ser Phe Gly Glu Asp Val Trp Lys Asn Leu
180 185 190
Tyr Arg Tyr Thr Tyr Asp Ile Tyr Thr Ile Arg Asn Ile Ser Thr His
195 200 205
Leu Thr Tyr Asp Leu Phe Lys Lys Glu Leu Arg Lys Gly Asn Phe Ile
210 215 220
Asp Ile Asp Gly Met Arg Phe Leu Ile Tyr Ala Tyr Val Asp Asp Lys
225 230 235 240
Arg Ser Leu Leu Tyr Met Arg Asn Ala Val Glu Gly Ile Leu Lys Phe
245 250 255
Tyr Arg Glu Lys Gly Tyr Ile Pro Leu Lys Tyr Asn Tyr Val Thr Gly
260 265 270
Gly Ala Ser Lys Leu Lys Ala Pro Phe Cys Phe Tyr Tyr Val Phe Ser
275 280 285
Lys Leu Leu Pro Ser Asp Lys Asn Leu Glu Lys Glu Phe Arg Asn Gly
290 295 300
Leu Glu Tyr Asp Lys Lys Asn Tyr Tyr Cys Tyr Ala Leu Leu Leu Ile
305 310 315 320
Ala Leu Leu His Asp
325
<210> 121
<211> 341
<212> PRT
<213> Gluconacetobacter xylinus
<400> 121
Met Ala Val Ala Gly Ser Phe Pro Met Leu Ser Ser Gly Ala Glu Ala
1 5 10 15
Asp Asp Ala Ile Gly Ile Asn Pro Gln Ile Ala Gln Gln Trp Ala Ile
20 25 30
Phe Arg Asp Lys Tyr Phe His Pro Asn Gly Arg Ile Ile Asp Thr Gly
35 40 45
Asn Ser Gly Glu Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu Phe
50 55 60
Ser Ala Ala Ala Gly Asp Gln Ala Ala Phe Glu Val Ile Trp Val Trp
65 70 75 80
Ala Arg Thr Asn Leu Gln His Lys Asp Asp Ala Leu Phe Ser Trp Arg
85 90 95
Tyr Leu Asp Gly His Lys Pro Pro Val Ala Asp Lys Asn Asn Ala Thr
100 105 110
Asp Gly Asp Leu Leu Ile Ala Leu Ala Leu Ala Trp Ala Gly Lys Arg
115 120 125
Trp Lys Arg Ala Asp Tyr Ile Gln Asp Ala Met Asn Ile Tyr Gly Asp
130 135 140
Val Leu Lys Leu Met Thr Lys Ser Val Gly Pro Tyr Thr Val Leu Leu
145 150 155 160
Pro Gly Ala Val Gly Phe Leu Thr Lys Asp Thr Val Thr Leu Asn Leu
165 170 175
Ser Tyr Tyr Val Met Pro Ser Leu Met Gln Ala Phe Ala Leu Thr Gly
180 185 190
Asp Ala Lys Trp Thr Lys Val Met Gly Asp Gly Leu Gln Ile Ile Ala
195 200 205
Lys Gly Arg Phe Gly Glu Trp Lys Leu Pro Pro Asp Trp Leu Ser Ile
210 215 220
Asn Leu His Thr Asn Ala Phe Ser Ile Ala Lys Gly Trp Pro Pro Arg
225 230 235 240
Phe Ser Tyr Asp Ala Ile Arg Val Pro Leu Tyr Leu Ser Trp Ala His
245 250 255
Met Leu Thr Pro Glu Leu Leu Ala Asp Phe Ser Arg Phe Trp Asn His
260 265 270
Tyr Gly Ala Ser Ala Leu Pro Gly Trp Val Asp Leu Thr Asn Gly Ala
275 280 285
Arg Ser Pro Tyr Asn Ala Pro Pro Gly Tyr Leu Ala Val Ala Ser Cys
290 295 300
Thr Gly Leu Ala Ser Ala Gly Glu Leu Pro Thr Leu Asp His Ala Pro
305 310 315 320
Asp Tyr Tyr Ser Ala Ala Leu Thr Met Leu Ala Tyr Ile Ala Arg Asn
325 330 335
Gln Gly Asp Gly Met
340
<210> 122
<211> 359
<212> PRT
<213> 潮湿纤维单胞菌
<400> 122
Met Pro Leu Arg Ala Leu Val Ala Val Ile Val Thr Thr Ala Val Met
1 5 10 15
Leu Val Pro Arg Ala Trp Ala Gln Thr Ala Trp Glu Arg Tyr Lys Ala
20 25 30
Arg Phe Met Met Pro Asp Ala Arg Ile Ile Asp Thr Ala Asn Gly Asn
35 40 45
Val Ser His Thr Glu Gly Gln Gly Phe Ala Met Leu Leu Ala Val Ala
50 55 60
Asn Asn Asp Arg Pro Ala Phe Asp Lys Leu Trp Gln Trp Thr Asp Ser
65 70 75 80
Thr Leu Arg Asp Lys Ser Asn Gly Leu Phe Tyr Trp Arg Tyr Asn Pro
85 90 95
Val Ala Pro Asp Pro Ile Ala Asp Lys Asn Asn Ala Thr Asp Gly Asp
100 105 110
Thr Leu Ile Ala Trp Ala Leu Leu Arg Ala Gln Lys Gln Trp Gln Asp
115 120 125
Lys Arg Tyr Ala Thr Ala Ser Asp Ala Ile Thr Ala Ser Leu Leu Lys
130 135 140
Tyr Thr Val Val Thr Phe Ala Gly Arg Gln Val Met Leu Pro Gly Val
145 150 155 160
Lys Gly Phe Asn Arg Asn Asp His Leu Asn Leu Asn Pro Ser Tyr Phe
165 170 175
Ile Phe Pro Ala Trp Arg Ala Phe Ala Glu Arg Thr His Leu Thr Ala
180 185 190
Trp Arg Thr Leu Gln Ser Asp Gly Gln Ala Leu Leu Gly Gln Met Gly
195 200 205
Trp Gly Lys Ser His Leu Pro Ser Asp Trp Val Ala Leu Arg Ala Asp
210 215 220
Gly Lys Met Leu Pro Ala Lys Glu Trp Pro Pro Arg Met Ser Phe Asp
225 230 235 240
Ala Ile Arg Ile Pro Leu Tyr Ile Ser Trp Val Asp Pro His Ser Ala
245 250 255
Leu Leu Ala Pro Trp Lys Ala Trp Met Gln Ser Tyr Pro Arg Leu Gln
260 265 270
Thr Pro Ala Trp Ile Asn Val Ser Thr Asn Glu Val Ala Pro Trp Asn
275 280 285
Met Ala Gly Gly Leu Leu Ala Val Arg Asp Leu Thr Leu Gly Glu Pro
290 295 300
Leu Glu Arg Arg Arg Leu Thr Thr Arg Met Ile Ile Thr Pro Pro Ala
305 310 315 320
Ser Ser Cys Trp Ser Gly Trp Arg Asn Arg Ile Ser Ala Ser Ala Val
325 330 335
Met Ala Leu Gln Val Ser Gln Pro Val Cys Leu Arg Ala Glu Arg Lys
340 345 350
Glu Gln Glu Arg Leu Thr Met
355
<210> 123
<211> 332
<212> PRT
<213> 菊欧文氏菌
<400> 123
Met Gly Lys Pro Met Trp Arg Cys Trp Ala Leu Met Leu Met Val Trp
1 5 10 15
Phe Ser Ala Ser Ala Thr Ala Ala Asn Gly Trp Glu Ile Tyr Lys Ser
20 25 30
Arg Phe Met Thr Thr Asp Gly Arg Ile Gln Asp Thr Gly Asn Lys Asn
35 40 45
Val Ser His Thr Glu Gly Gln Gly Phe Ala Met Leu Met Ala Val His
50 55 60
Tyr Asp Asp Arg Ile Ala Phe Asp Asn Leu Trp Asn Trp Thr Gln Ser
65 70 75 80
His Leu Arg Asn Thr Thr Ser Gly Leu Phe Tyr Trp Arg Tyr Asp Pro
85 90 95
Ser Ala Ala Asn Pro Val Val Asp Lys Asn Asn Ala Ser Asp Gly Asp
100 105 110
Val Leu Ile Ala Trp Ala Leu Leu Lys Ala Gly Asn Lys Trp Gln Asp
115 120 125
Asn Arg Tyr Leu Gln Ala Ser Asp Ser Ile Gln Lys Ala Ile Ile Ala
130 135 140
Ser Asn Ile Ile Gln Phe Ala Gly Arg Thr Val Met Leu Pro Gly Ala
145 150 155 160
Tyr Gly Phe Asn Lys Asn Ser Tyr Val Ile Leu Asn Pro Ser Tyr Phe
165 170 175
Leu Phe Pro Ala Trp Arg Asp Phe Ala Asn Arg Ser His Leu Gln Val
180 185 190
Trp Arg Gln Leu Ile Asp Asp Ser Leu Ser Leu Val Gly Glu Met Arg
195 200 205
Phe Gly Gln Val Gly Leu Pro Thr Asp Trp Ala Ala Leu Asn Ala Asp
210 215 220
Gly Ser Met Ala Pro Ala Thr Ala Trp Pro Ser Arg Phe Ser Tyr Asp
225 230 235 240
Ala Ile Arg Ile Pro Leu Tyr Leu Tyr Trp Tyr Asp Ala Lys Thr Thr
245 250 255
Ala Leu Val Pro Phe Gln Leu Tyr Trp Arg Asn Tyr Pro Arg Leu Thr
260 265 270
Thr Pro Ala Trp Val Asp Val Leu Ser Ser Asn Thr Ala Thr Tyr Asn
275 280 285
Met Gln Gly Gly Leu Leu Ala Val Arg Asp Leu Thr Met Gly Asn Leu
290 295 300
Asp Gly Leu Ser Asp Leu Pro Gly Ala Ser Glu Asp Tyr Tyr Ser Ser
305 310 315 320
Ser Leu Arg Leu Leu Val Met Leu Ala Arg Gly Lys
325 330
<210> 124
<211> 463
<212> PRT
<213> 芽孢杆菌属菌种KSM-330
<400> 124
Met Val Glu Lys Arg Lys Ile Phe Thr Val Leu Cys Ala Cys Gly Ile
1 5 10 15
Gly Phe Thr Ser Tyr Thr Ser Cys Ile Ser Ala Ala Ala Ile Asp Asn
20 25 30
Asp Thr Leu Ile Asn Asn Gly His Lys Ile Asn Ser Ser Ile Ile Thr
35 40 45
Asn Ser Ser Gln Val Ser Ala Val Ala Lys Glu Met Lys Pro Phe Pro
50 55 60
Gln Gln Val Asn Tyr Ser Gly Ile Leu Lys Pro Asn His Val Ser Gln
65 70 75 80
Glu Ser Leu Asn Asn Ala Val Lys Asn Tyr Tyr Asn Asp Trp Lys Lys
85 90 95
Lys Tyr Leu Lys Asn Asp Leu Ser Ser Leu Pro Gly Gly Tyr Tyr Val
100 105 110
Lys Gly Glu Ile Thr Gly Asn Pro Asp Gly Phe Arg Pro Leu Gly Thr
115 120 125
Ser Glu Gly Gln Gly Tyr Gly Met Ile Ile Thr Val Leu Met Ala Gly
130 135 140
His Asp Ser Asn Ala Gln Thr Ile Tyr Asp Gly Leu Phe Lys Thr Ala
145 150 155 160
Arg Ala Phe Lys Ser Ser Ile Asn Pro Asn Leu Met Gly Trp Val Val
165 170 175
Ala Asp Asp Lys Lys Ala Gln Gly His Phe Asp Ser Ala Thr Asp Gly
180 185 190
Asp Leu Asp Ile Ala Tyr Ser Leu Leu Leu Ala His Lys Gln Trp Gly
195 200 205
Ser Ser Gly Lys Ile Asn Tyr Leu Lys Glu Ala Gln Asn Met Ile Thr
210 215 220
Lys Gly Ile Lys Ala Ser Asn Val Thr Lys Asn Asn Gly Leu Asn Leu
225 230 235 240
Gly Asp Trp Gly Asp Lys Ser Thr Phe Asp Thr Arg Pro Ser Asp Trp
245 250 255
Met Met Ser His Leu Arg Ala Phe Tyr Glu Phe Thr Gly Asp Lys Thr
260 265 270
Trp Leu Asn Val Ile Asp Asn Leu Tyr Asn Thr Tyr Thr Asn Phe Thr
275 280 285
Asn Lys Tyr Ser Pro Lys Thr Gly Leu Ile Ser Asp Phe Val Val Lys
290 295 300
Asn Pro Pro Gln Pro Ala Pro Lys Asp Phe Leu Asp Glu Ser Lys Tyr
305 310 315 320
Thr Asp Ser Tyr Tyr Tyr Asn Ala Ser Arg Val Pro Leu Arg Ile Val
325 330 335
Met Asp Tyr Ala Met Tyr Gly Glu Lys Arg Gly Lys Val Ile Ser Asp
340 345 350
Lys Val Ala Thr Trp Ile Lys Ser Lys Thr Lys Gly Asn Pro Ser Lys
355 360 365
Ile Val Asp Gly Tyr Lys Leu Asp Gly Thr Asn Ile Gly Asp Tyr Pro
370 375 380
Thr Ala Val Tyr Val Ser Pro Phe Ile Ala Ala Gly Thr Thr Asn Ser
385 390 395 400
Lys Asn Gln Glu Trp Val Asn Ser Gly Trp Asp Trp Met Lys Asn Lys
405 410 415
Lys Glu Ser Tyr Phe Ser Asp Ser Tyr Asn Leu Leu Thr Met Leu Phe
420 425 430
Leu Thr Gly Asn Trp Trp Lys Pro Ile Pro Asp Glu Lys Lys Ile Gln
435 440 445
Ser Pro Ile Asn Leu Glu Val Gln Ser Glu Leu Lys Glu Gln Asp
450 455 460
<210> 125
<211> 342
<212> PRT
<213> 木醋杆菌
<400> 125
Met Ser Val Met Ala Ala Met Gly Gly Ala Gln Val Leu Ser Ser Thr
1 5 10 15
Gly Ala Phe Ala Asp Pro Ala Pro Asp Ala Val Ala Gln Gln Trp Ala
20 25 30
Ile Phe Arg Ala Lys Tyr Leu Arg Pro Ser Gly Arg Val Val Asp Thr
35 40 45
Gly Asn Gly Gly Glu Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu
50 55 60
Phe Ala Ala Ser Ala Gly Asp Leu Ala Ser Phe Gln Ser Met Trp Met
65 70 75 80
Trp Ala Arg Thr Asn Leu Gln His Thr Asn Asp Lys Leu Phe Ser Trp
85 90 95
Arg Phe Leu Lys Gly His Gln Pro Pro Val Pro Asp Lys Asn Asn Ala
100 105 110
Thr Asp Gly Asp Leu Leu Ile Ala Leu Ala Leu Gly Arg Ala Gly Lys
115 120 125
Arg Phe Gln Arg Pro Asp Tyr Ile Gln Asp Ala Met Ala Ile Tyr Gly
130 135 140
Asp Val Leu Asn Leu Met Thr Met Lys Ala Gly Pro Tyr Val Val Leu
145 150 155 160
Met Pro Gly Ala Val Gly Phe Thr Lys Lys Asp Ser Val Ile Leu Asn
165 170 175
Leu Ser Tyr Tyr Val Met Pro Ser Leu Leu Gln Ala Phe Asp Leu Thr
180 185 190
Ala Asp Pro Arg Trp Arg Gln Val Met Glu Asp Gly Ile Arg Leu Val
195 200 205
Ser Ala Gly Arg Phe Gly Gln Trp Arg Leu Pro Pro Asp Trp Leu Ala
210 215 220
Val Asn Arg Ala Thr Gly Ala Leu Ser Ile Ala Ser Gly Trp Pro Pro
225 230 235 240
Arg Phe Ser Tyr Asp Ala Ile Arg Val Pro Leu Tyr Phe Tyr Trp Ala
245 250 255
His Met Leu Ala Pro Asn Val Leu Ala Asp Phe Thr Arg Phe Trp Asn
260 265 270
Asn Phe Gly Ala Asn Ala Leu Pro Gly Trp Val Asp Leu Thr Thr Gly
275 280 285
Ala Arg Ser Pro Tyr Asn Ala Pro Pro Gly Tyr Leu Ala Val Ala Glu
290 295 300
Cys Thr Gly Leu Asp Ser Ala Gly Glu Leu Pro Thr Leu Asp His Ala
305 310 315 320
Pro Asp Tyr Tyr Ser Ala Ala Leu Thr Leu Leu Val Tyr Ile Ala Arg
325 330 335
Ala Glu Glu Thr Ile Lys
340
<210> 126
<211> 384
<212> PRT
<213> 地毯草黄单胞菌
<400> 126
Met Thr Arg Arg Arg Leu Leu His Ala Gly Thr Leu Ala Gly Val Ala
1 5 10 15
Ala Leu Leu Pro Ala Ala Ala Leu Ala Ala Pro Ser Gln Cys Gly Pro
20 25 30
Trp Pro Leu Trp Ser Ala Phe Val Asp Lys His Ile Gln Arg Asp Gly
35 40 45
Arg Val Val Asp Phe Leu Asn Pro Asp Gln Arg Ser Thr Ser Glu Gly
50 55 60
Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Asn Asn Asp Gln Val Leu
65 70 75 80
Phe Asp Lys Val Leu Ser Trp Thr Arg His Asn Leu Cys Gly Gly Arg
85 90 95
Pro Asp Leu Asn Leu Pro Ala Trp Leu Trp Gly Arg Asp Gly Ser Gly
100 105 110
Ala Trp Arg Val Leu Asp Ala Asn Thr Ala Ser Asp Gly Glu Leu Trp
115 120 125
Ile Ala Tyr Ala Leu Leu Glu Ala Gly Arg Leu Trp Ser Arg Pro Gly
130 135 140
Phe Leu Lys Ala Gly Gln Gln Met Leu Gln Leu Ile Arg Thr Gln Glu
145 150 155 160
Val Ala Thr Leu Pro Gly Leu Gly Pro Met Leu Leu Pro Gly Arg Thr
165 170 175
Gly Phe Val Asp Asn Gly Arg Trp Thr Leu Asn Pro Ser Tyr Leu Pro
180 185 190
Ile Gln Val Leu Arg Arg Cys Ala Asn Ala Asp Pro Lys Gly Pro Trp
195 200 205
Ala Ala Ile Ala Ala Asn Ser Ala Arg Val Leu Arg Asp Ser Ala Pro
210 215 220
Val Gly Phe Ala Pro Asp Trp Thr Val Trp Asp Gly Lys Thr Phe Asn
225 230 235 240
Ala Asp Pro Lys Arg Gly Asn Val Gly Ser Tyr Asp Ala Ile Arg Val
245 250 255
Tyr Leu Trp Ala Gly Met Leu Asp Ala Gly Glu Pro Leu Arg Ala Arg
260 265 270
Leu Leu Gln Asp Leu Ser Gly Pro Ala Asp Leu Leu Ala Ala Gln Gln
275 280 285
Thr Pro Ala Glu Lys Ile Asp Thr Ala Arg Gly Val Gly Thr Gly Ala
290 295 300
Leu Pro Val Gly Phe Ser Ala Ala Leu Leu Pro Tyr Leu Ser Ala Leu
305 310 315 320
Gly Lys Pro Ala Leu Leu Lys Ala Gln Ala Gln Arg Val Pro Ala Ala
325 330 335
Thr Gln Pro Ala Ala Ala Ala Leu Pro Tyr Phe Glu Arg Thr Leu Ala
340 345 350
Leu Phe Gly Gln Gly Trp Leu Glu Asn Arg Tyr Arg Phe Ala Ala Asp
355 360 365
Gly Arg Leu Leu Pro Ala Trp Arg Thr Pro Ala Cys Ala Ala Thr Thr
370 375 380
<210> 127
<211> 365
<212> PRT
<213> Fulvimarina pelagi
<400> 127
Met Lys Lys Tyr Leu Leu Phe Phe Cys Ala Ala Ala Phe Leu Met Ser
1 5 10 15
Gly Thr Ile Asn Ser Arg Ala Val Glu Pro His Thr Glu Asn Thr Glu
20 25 30
Ser Pro Asn Ile Glu Ala Ala Ser Gly Asp Leu Ala Leu Gly Ala Trp
35 40 45
Gln Phe Tyr Arg Ser Val Phe Met Ser Ala Asp Gly Arg Ile Ile Asp
50 55 60
Arg Glu Asn Gly Ser Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met
65 70 75 80
Leu Ile Ala Ala Met Val Gly Asp Arg Ala Ser Phe Asp Lys Leu Trp
85 90 95
Asp Trp Thr Glu Arg Glu Leu Gln Ile Arg Asp Asp Asn Leu Ser Ala
100 105 110
Trp Lys Trp Asp Pro Ser Ala Thr Pro His Val Thr Asp Arg Asn Asn
115 120 125
Ala Thr Asp Gly Asp Ile Leu Ile Ala Trp Ala Leu Leu Arg Ala Phe
130 135 140
Glu Arg Trp Gly Val Pro Asp His Glu Arg Glu Ala Glu Ala Ile Ile
145 150 155 160
Ala Asp Ile Val Arg Leu Ala Ile Ile Asp Asp Arg Gln Gly Lys Ile
165 170 175
Ile Leu Pro Gly Val Glu Gly Phe Asp Ala Asp Ser Gln Pro Asp Gly
180 185 190
Pro Val Val Asn Leu Ser Tyr Trp Val Phe Pro Ala Leu Ala Glu Ile
195 200 205
Gly Gln His Met Pro Asp Phe Gly Ala Leu Asn Leu Glu Ala Lys Gly
210 215 220
Leu Glu Leu Leu Lys Glu Ala Thr Ala Ser Ser Pro His Leu Pro Thr
225 230 235 240
Glu Trp Ser Gly Leu Gly Ser Gly Thr Val Lys Pro Ala Ala Gln Phe
245 250 255
Asp Pro Phe Phe Gly Tyr Asn Ala Leu Arg Ile Pro Leu Tyr Leu Ala
260 265 270
Trp Ser Glu Ile Glu Ala Pro Glu Ile Leu Lys Arg Ile Asp Lys Ala
275 280 285
Trp Glu Gly Lys Thr Glu Thr Gly Leu Ala Val Ile Asp Val Ala Arg
290 295 300
Asp Ala Pro Val Glu Pro Ile Ser Gly Ala Gly Tyr Gln Ala Ile Arg
305 310 315 320
Glu Leu Val Gln Cys Ser Leu Asn Ser Ile Ala Ala Gly Arg Glu Arg
325 330 335
Ala Leu Phe Asp Gly Ser Thr Tyr Tyr Ser Ser Thr Leu His Leu Leu
340 345 350
Ser Leu Leu Ala Ile Ser Glu Arg Tyr Pro Arg Cys Phe
355 360 365
<210> 128
<211> 376
<212> PRT
<213> Chlorobium ferrooxidans
<400> 128
Met Arg Thr Leu Phe Pro Leu Leu Leu Leu Ser Leu Leu Pro Leu Leu
1 5 10 15
Ser Ser Ser Cys Ser Ala Thr Pro Val Asp Pro Glu Glu Ile Val Arg
20 25 30
Gly Ser Trp Val His Tyr Lys Arg Thr Phe Ile Thr Glu Gly Arg Val
35 40 45
Val Arg Pro Gln Asn Ser Asn Asp Thr Val Ser Glu Gly Glu Ala Tyr
50 55 60
Ala Met Leu Arg Ala Val Leu Met Asp Asp Arg Lys Thr Phe Asp Glu
65 70 75 80
Cys Leu Ala Trp Ser Glu Ala Thr Leu Ser Arg Lys Glu Ser His Gly
85 90 95
Asp Asp Leu Leu Ala Trp His Phe Glu Asn Gly Arg Val Ser Asp Ser
100 105 110
Thr Ala Ala Ser Asp Ala Asp Ile Asp Tyr Ala Tyr Ser Leu Leu Leu
115 120 125
Ala Trp Arg Thr Trp Gln Glu Ser Arg Tyr Leu Asp Leu Ala Arg Lys
130 135 140
Val Leu Gln Ser Ile Leu Asp Lys Glu Thr Val Leu Val Asn Asn Arg
145 150 155 160
Leu Tyr Leu Leu Pro Trp Pro Ala Gly Glu Gly Gly Ser Thr Pro Pro
165 170 175
Asp Ser Thr Pro Gln Asn Pro Ser Tyr Tyr Ala Pro Ser His Phe Arg
180 185 190
Leu Phe Tyr Ala Val Thr Gly Asp Lys Arg Trp Met Glu Leu Val Asp
195 200 205
Thr Thr Tyr Glu Leu Leu Gly Arg Leu Gln Lys Ser Asn Asp Asn Pro
210 215 220
Gly Leu Val Pro Asp Trp Cys Ala Leu Asp Met Gln Glu Asn Ile Ile
225 230 235 240
Pro Met Pro Gly Lys Lys Arg Val Tyr Gly Trp Asp Ala Val Arg Val
245 250 255
Pro Leu Arg Val Ala Ala Asp Tyr Arg Leu Asn Asp Asp His Arg Ala
260 265 270
Leu Gln Val Leu Arg Ile Phe Ser Glu Phe Phe Glu Arg Glu Phe Arg
275 280 285
Glu Asn Gly Lys Ile Val Thr Gly Tyr Ser Gly Ser Asp Ala Thr Lys
290 295 300
Ser Ser Met Glu Asn Pro Leu Phe Tyr Ala Ala Ala Tyr Ala Ala Thr
305 310 315 320
Glu Ala Ser Asp Ser Pro Ile Ala Pro Glu Met Leu Gln Arg Val Arg
325 330 335
Asp Phe Ile His Gln Asn Ser Asp Glu Tyr Tyr Tyr Asn Glu Ala Asn
340 345 350
Asp Tyr Tyr Val Asn Ser Leu Val Trp Leu Thr Glu Tyr Phe Gln Ile
355 360 365
Thr Lys Lys Thr Ala Thr Asn Asp
370 375
<210> 129
<211> 1223
<212> PRT
<213> 哈氏噬纤维菌
<400> 129
Met Ile Lys Leu Met Asn Lys Ile Ser Trp Val Val Ile Ser Val Leu
1 5 10 15
Cys Leu Ile His Val Gln Thr His Ala Gln Ile Asn Thr Pro Ala Gly
20 25 30
Ala Thr Val Pro Phe Gly Ser Asn Asn Ser Tyr Ala Tyr Gly Ile Leu
35 40 45
Pro Thr Asn Leu Pro Thr Ser Gly Thr Tyr Gly Lys Gly Thr Glu Val
50 55 60
Ala Ala Lys Tyr Thr Ala Trp Lys Ser Ala Tyr Ile Glu Asn Cys Gly
65 70 75 80
Thr Asp Lys Ala Arg Val Lys Phe Asp Asn Thr Ser Glu Thr Val Ser
85 90 95
Glu Gly Ile Ala Tyr Gly Met Leu Leu Ala Ala Tyr Ala Ala Asp Gln
100 105 110
Asp Leu Phe Asn Arg Leu Trp Ala Tyr Tyr Lys Gln His Arg Asn Ala
115 120 125
Lys Gly Val Met His Trp Lys Ile Ser Gly Cys Asn Ser Val Ile Gly
130 135 140
Gln Asn Gly Ala Thr Asp Ala Glu Leu Asp Ala Ala Met Ala Leu Ile
145 150 155 160
Val Ala Asn Tyr Gln Trp Pro Asn Thr Thr Ser Pro His Asn Tyr Lys
165 170 175
Thr Asp Ala Val Ser Leu Ile Asn Ala Ile Lys Asn Tyr Glu Ile Asn
180 185 190
Ala Thr Asp Phe Thr Phe Glu Asn Gly Asp Ala Trp Lys Pro Ala Cys
195 200 205
Arg Asn Pro Ser Tyr Gln Ala Pro Gly Tyr Ala Arg Val Phe Lys Leu
210 215 220
Phe Met Ala Glu Asn Gly Gln Ala Asn Asn Thr Phe Trp Asp Asn Val
225 230 235 240
Ile Gln Lys Thr Glu Asn Leu Leu Ile Asn Asn Ala His Thr Thr Ser
245 250 255
Gly Leu Ser Thr Asn Trp Cys Thr Pro Ala Gly Pro Pro Asn Ser Ser
260 265 270
Cys Ser Gly Ser Gly Thr Ala Pro Asp Lys Phe Gly Tyr Asp Ala Cys
275 280 285
Arg Ala Pro Trp Arg Gln Ala Thr Asp Tyr Ile Trp Tyr Gly Pro Ser
290 295 300
Thr Ile Leu Thr Ile Thr Asn Arg Gln Ala Ala Phe Trp Ile Gly Lys
305 310 315 320
Gly Gly Ala Gly Ser Val Gln Gly Gly Asp Gly Val Asn His Asp Gly
325 330 335
Ser Gly Ser Gly Asn His Asn Ala Ala Phe Val Gly Pro Ile Gly Ala
340 345 350
Leu Ser Leu Ala Thr Thr Asn Thr Thr Ala Asn Gln Gln Phe Cys Asn
355 360 365
Ala Leu Tyr Ser Glu Asn Lys Asn Asp Gly Leu Ala Ser Gly Tyr Phe
370 375 380
Thr Lys Ile Leu Gln Met Leu Gly Leu Phe Val Gln Thr Gly Asn Phe
385 390 395 400
Trp Asn Pro Tyr Ala Val Ser Ser Ala Thr Thr Thr Ser Val Ala Leu
405 410 415
Thr Ala Pro Thr Val Thr Phe Ala Ala Glu Gly Asp Val Ile Thr Val
420 425 430
Ser Ala Thr Ala Thr Ala Thr Thr Gly Ala Ile Ser Lys Val Asp Phe
435 440 445
Tyr Ala Gly Thr Thr Leu Ile Gly Thr Asp Asn Ser Ser Pro Tyr Ser
450 455 460
Ile Ser Trp Thr Pro Asn Val Ser Gly Thr Val Ser Ile Thr Ala Lys
465 470 475 480
Ala Thr Asn Thr Asn Gly Asp Val Ala Thr Thr Ala Pro Phe Asn Val
485 490 495
Ser Ile Tyr Lys Ala Val Asn Gln Thr Asn Thr Ala Pro Thr Ile Asp
500 505 510
Gly Val Ala Asp Gly Val Trp Gly Gly Thr Asn Thr Ser Ala Ser Leu
515 520 525
Asn Asn Val Ile Asp Gly Thr Val Ala Asn Ala Ser Asp Leu Ser Ala
530 535 540
Asn Tyr Lys Ala Met Trp Asp Ala Thr Tyr Phe Tyr Val Leu Val Asn
545 550 555 560
Val Thr Asp Asn Val Lys Thr Asn Asn Gly Gly Thr Asp Tyr Tyr Asn
565 570 575
Asp Asp Ala Val Glu Val Phe Phe Asp Ile Gly Asn Asn Lys Thr Thr
580 585 590
Thr Tyr Gly Ala Asn Asp Phe Gln Tyr Thr Phe Arg Trp Asn Asp Asn
595 600 605
Thr Ile Tyr Glu Lys Asn Asn Lys Thr Thr Gly Val Thr Phe Ala Arg
610 615 620
Val Asp Asn Ser Thr Gly Tyr Val Met Glu Met Arg Phe Pro Trp Ser
625 630 635 640
Thr Leu Thr Gly Ser Pro Ala Ile Asn Gln Leu Val Gly Phe Asp Val
645 650 655
Ala Val Asn Asp Asp Asp Asp Gly Asn Ala Arg Asp Arg Lys Ile Ser
660 665 670
Trp Ala Ala Thr Ala Asp Gln Ala Trp Thr Asn Pro Ser Tyr Met Gly
675 680 685
Thr Val Ile Leu Lys Gly Ala Pro Ala Cys Thr Leu Pro Ser Ala Ala
690 695 700
Gly Thr Ile Thr Gly Thr Thr Thr Thr Cys Ala Asn Ala Ala Gly Ile
705 710 715 720
Thr Tyr Ser Ile Ala Ala Val Thr Gly Ala Thr Gly Tyr Thr Trp Thr
725 730 735
Val Pro Ala Gly Ala Thr Ile Thr Ala Gly Ala Asn Thr Thr Ala Ile
740 745 750
Thr Val Lys Phe Gly Thr Thr Ala Gly Asn Ile Ser Val Thr Pro Ala
755 760 765
Asn Thr Cys Gly Asn Gly Thr Ala Ala Thr Thr Ala Val Thr Leu Asn
770 775 780
Ser Leu Pro Ser Ala Ala Gly Ser Ile Thr Gly Ile Thr Thr Thr Cys
785 790 795 800
Ala Asn Ala Ala Gly Ile Thr Tyr Ser Ile Ala Ala Val Thr Gly Ala
805 810 815
Thr Gly Tyr Asn Trp Thr Val Pro Ala Gly Ala Thr Ile Thr Ser Gly
820 825 830
Thr Asn Thr Arg Thr Ile Thr Val Thr Phe Gly Thr Thr Gly Gly Asn
835 840 845
Val Ser Val Thr Pro Thr Asn Thr Cys Gly Asn Gly Thr Ala Ala Ser
850 855 860
Thr Ala Val Thr Val Asn Ser Leu Pro Ala Ala Ala Gly Asp Ile Thr
865 870 875 880
Gly Val Thr Thr Thr Cys Ala Asn Thr Ala Gly Val Thr Tyr Ser Ile
885 890 895
Ala Ala Val Thr Gly Ala Thr Gly Tyr Ser Trp Thr Val Pro Ala Gly
900 905 910
Ala Thr Ile Thr Ala Gly Ala Asn Thr Arg Thr Ile Thr Val Thr Phe
915 920 925
Gly Thr Ala Gly Gly Asn Val Thr Val Thr Pro Thr Asn Thr Cys Gly
930 935 940
Asn Gly Thr Asn Lys Thr Thr Ala Val Thr Val Asn Ala Ser Gly Gly
945 950 955 960
Asn Ala Ser Val Ser Ile Ala Ala Ser Pro Ser Ser Thr Ile Tyr Thr
965 970 975
Gly Gln Ser Val Thr Phe Thr Ala Thr Pro Val Asn Gly Gly Thr Pro
980 985 990
Thr Tyr Gln Trp Lys Asn Gly Ser Thr Asn Ile Ala Gly Ala Thr Ala
995 1000 1005
Ala Thr Tyr Thr Thr Thr Ser Leu Thr Asn Gly Asn Ala Ile Ser
1010 1015 1020
Val Thr Met Thr Ser Thr Lys Gln Cys Val Ala Asn Thr Thr Ala
1025 1030 1035
Asn Ser Asn Thr Ile Thr Met Thr Val Thr Ala Ala Pro Ala Phe
1040 1045 1050
Ser Ser Thr Ile Asn Gly Pro Thr Val Val Ser Pro Asn Gln Ser
1055 1060 1065
Asn Val Thr Phe Ser Val Ser Asn Gln Ser Gly Met Gln Tyr Gln
1070 1075 1080
Trp Thr Val Pro Pro Gly Ala Thr Ile Val Ser Gly Ala Asn Thr
1085 1090 1095
Asn Ser Ile Val Val Asn Phe Gly Thr Ser Gly Gly Asn Val Thr
1100 1105 1110
Val Lys Glu Thr Asn Pro Ser Asn Gln Thr Thr Thr Ile Thr Lys
1115 1120 1125
Ala Ile Thr Val Ser Gly Thr Thr Pro Val Tyr Leu Pro Ala Gln
1130 1135 1140
Glu Val Asn Ile Ser Val Tyr Pro Val Pro Cys Asn Glu Gln Leu
1145 1150 1155
Met Ile Ala Met Gly Val Glu Ala Ser Thr Lys Val Ser Phe Ser
1160 1165 1170
Ile Ile Asp Met Thr Gly Asn Thr Ile Gln Ser Gly Ser Phe Glu
1175 1180 1185
Tyr Asn Ser Met Pro Val Ser Ile Glu Met Pro Val Ala Ala Gly
1190 1195 1200
Ile Tyr Gln Leu Leu Leu Gln Trp Asp Gly Thr Tyr Ala Met His
1205 1210 1215
Lys Ile Met Lys Gln
1220
<210> 130
<211> 912
<212> PRT
<213> Pseudoalteromonas atlantica
<400> 130
Met Ser Thr Leu Ser Val Ser Asn Thr Val Ser Ser Ala Pro Ser Gly
1 5 10 15
Lys Thr Val Ser Leu Ser Lys Ser Val Ala Leu Ala Leu Gly Thr Leu
20 25 30
Leu Leu Thr Ser Phe Ser Met Pro Ser Tyr Ala Glu Val Ser Ile Asp
35 40 45
His Leu Thr Leu Thr Asn Lys Thr Arg Ile Gly Arg Ser Asp Tyr Arg
50 55 60
Tyr Glu Phe Ser Val Phe Val Lys Asn Asp Ala Glu Arg Val Gln Asp
65 70 75 80
Val Gln Ile Asp Val Thr Ser Ser Asn Pro His Ser Arg Ile Glu Ser
85 90 95
Ser Gly Val Ser Leu Ile Glu Ile Pro Ala Asn Ser Val Leu Glu Leu
100 105 110
Thr Glu Pro Phe Val Leu Val Gln Asn Arg Arg Ala Arg Phe Asn Glu
115 120 125
Gln Asp Leu Ser Trp Val Ile Asp Tyr Gln Gln Ser Gly Asn Ala Ile
130 135 140
Ser Asp Asp Phe Ser Pro Asn Ala Ala Tyr His Thr Gly Glu Tyr Lys
145 150 155 160
Asn Phe Phe Val Glu Arg Gly Leu Ala Thr Gln Glu Gln Val Thr Ala
165 170 175
Lys Ile Asp Ala Thr Tyr Gln Gln Leu Phe Glu Leu Val Pro Asp Gln
180 185 190
Ala Leu Pro Gln Pro Ser Lys Gly Glu Arg Ile Tyr His Ala Gly Phe
195 200 205
Asp Asp Ser Ile Asp Asn Trp Gln Trp His Ala Asp Asn Gly Ser Gln
210 215 220
Val Gly Ala Thr Leu Ser Gln His Gly Gly Ala Leu Ile Ile Thr Pro
225 230 235 240
Asn Trp Gln Ser Gly Gly Asp Ala Leu Ala Val Leu Ser Ser Ser Phe
245 250 255
Glu Pro Ile Asp Ala Thr Gln Gly Val Asp Val Glu Tyr Lys Met Ala
260 265 270
Val Asp Gln Ala Tyr Ala Asp Asp Gly Ala Met Ala Ala Gln Leu Phe
275 280 285
Ile Gln Asp Ser Ser Gly Gly Ile Gly Phe Phe Ala Tyr Arg Thr Leu
290 295 300
Thr Gln Pro Asp Glu Ser Thr Ile Leu Val Lys Asp Leu Gly Pro Asp
305 310 315 320
Thr Asn Phe Gly Tyr Leu Ser Glu Gly Phe Asp Phe Ser Gln Ile Ser
325 330 335
Thr Leu Gly Phe Gln Phe Leu Ala Asn Gly Lys Ser Ala Asp Ile Gly
340 345 350
Gly Asp Ile Thr Val Ser Glu Val Ser Val Tyr Gln Pro Val Gly Gly
355 360 365
Ser Glu Thr Asp Asn Ser Pro Ser Phe Leu Asp Ser Phe Asp His Gly
370 375 380
Ile Ala Gln Trp Asn Ala Gly Ala Asn Asn Gly Ser Ala Ile Glu Pro
385 390 395 400
Ala Leu Thr Val Gly Asn Val Ile Gly Gly Asp Gly Gln Ser Asn Asn
405 410 415
Val Leu Leu Ile Ser Pro Asn Trp Leu Ser Asp Ser Asp Val Phe Thr
420 425 430
Val Lys Tyr Gln Gln Phe Ala Ala Val Asp Ile Thr Asp Gly Arg Asp
435 440 445
Ile Ser Phe Asp Val Lys Ile Pro Ala Asn Tyr Val Val Asp Gly Asn
450 455 460
Leu Val Val Gln Leu Val Leu Glu Asp Ala Asn Tyr Gln Pro Ala Phe
465 470 475 480
Leu Gly Tyr Ile Lys Val Asp Gly Phe Pro Lys Asp Glu Phe Ile Thr
485 490 495
Leu Ser Phe Ser Asp Val Ser Ala Ala Thr Glu Phe Gly Tyr Ile Ser
500 505 510
Asp Ser Phe Asp Phe Ser Gln Leu Arg Gly Ile Gly Val Gln Phe Leu
515 520 525
Ala Asn Gly Lys Leu Pro Glu Leu Thr Gly Asp Ile Gln Ile Asp Asn
530 535 540
Val Leu Val Ala Gly Gln Ala Pro Ser Glu Pro Ala Pro Glu Leu Ser
545 550 555 560
Asp Thr Thr Val Leu Tyr Gly Val Gly Asp Asp Met Ala Phe Ile Lys
565 570 575
Ala Ile Asp Ser Asn Asp Ile Arg Ser Glu Gly Met Ser Tyr Gly Met
580 585 590
Met Ile Ser Val Met Met Asp Asp Gln Glu Thr Phe Asn Lys Leu Trp
595 600 605
Arg Phe Thr Lys Ala Lys Met Gln Asn Thr Ser Gly Asn Ser Lys Asp
610 615 620
Phe Phe Ala Trp Arg Leu Ser Ala Asn Ala Pro Tyr Asn Ala Ile Asp
625 630 635 640
Thr Asn Pro Ala Pro Asp Gly Glu Glu Tyr Phe Ala Met Ala Leu Phe
645 650 655
Phe Ala Asn Asn Arg Trp Gly Ser Ala Asp Gly Ile Phe Asp Tyr Gln
660 665 670
Arg Glu Ala Asn Asp Ile Leu His Asp Met Ile Phe Thr Lys Ser Asp
675 680 685
Asn Ser Ser Thr Arg Leu Met Met His Pro Val Tyr Gln Gln Val Glu
690 695 700
Phe Val Thr Thr Thr Asn Val Ala Ser Phe Ser Asp Pro Ser Tyr His
705 710 715 720
Leu Pro Ala Phe Tyr Glu Met Trp Ala Leu Trp Ala Asp Glu Asn Asn
725 730 735
Asp Tyr Trp His Glu Thr Ala Ala Ile Ser Arg Gln Tyr Leu Ala Lys
740 745 750
Ser Ala His Pro Val Thr Gly Leu Phe Ser Asp Tyr Ala Asn His Glu
755 760 765
Gly Glu Pro Gln Thr Thr Ser Phe Asn Pro Asp Ser His Lys Ser Ala
770 775 780
Tyr Asp Ser Phe Arg Val Met Gly Asn Met Ala Met Asp Tyr His Trp
785 790 795 800
Val Ser Gln Ser Pro Val Leu Gln Ser Leu Val Glu Gln Gln Val Thr
805 810 815
Phe Phe Ala Gly Glu Val Glu Gln Tyr Gly Asp Phe Ile Ala Val Tyr
820 825 830
Glu Val Asp Gly Thr Arg Glu Pro Gly Ile Asp Tyr Arg Ser His Gly
835 840 845
Arg Thr Ala Met Asn Gly Val Gly Ala Thr Ile Ser Glu Asn Pro Phe
850 855 860
Ser Thr Glu Met Leu Asn Tyr Leu Trp Gln Gln Asp Ala Pro Thr Gly
865 870 875 880
Met Tyr Arg Tyr Tyr Asp Gly Leu Leu His Met Phe Gly Leu Leu His
885 890 895
Ala Gly Gly Glu Phe Lys Ile Tyr Lys Pro Asn Glu Asn Glu Pro Glu
900 905 910
<210> 131
<211> 292
<212> PRT
<213> Deinococcus geothermalis
<400> 131
Met Lys Arg Ser Lys Thr His Leu Ala Val Val Gly Leu Gly Leu Leu
1 5 10 15
Ser Leu Leu Gly Ser Cys Gly Gln Ser Val Pro Gly Pro Glu Gln Asn
20 25 30
Ala Ala Phe Tyr Thr Gly Lys Tyr Arg Asn Leu Phe Thr Glu Trp Ser
35 40 45
Ile Ala Thr Glu Ala Gln Val Gln Ala Lys Leu Asp Ala Tyr Trp Glu
50 55 60
Ser Leu Phe Ala Ser Thr Asp Asp Gln Arg Arg Val Tyr Tyr Pro Ala
65 70 75 80
Gly Ser Asn Ala Asn Gly Pro Met Ala Tyr Val Ala Asp Ile Gly Ser
85 90 95
Asn Asp Val Arg Thr Glu Gly Met Ser Tyr Gly Met Met Ile Ala Val
100 105 110
Gln Met Asn Lys Gln Ala Glu Phe Asn Ala Leu Trp Asn Tyr Ala Lys
115 120 125
Ser Lys Met Gln His Gln Ser Gly Pro Arg Ala Gly Tyr Phe Ala Trp
130 135 140
His Thr Asp Phe Glu Gly Asn Ile Ile Asp Ala Asn Pro Ala Ser Asp
145 150 155 160
Gly Glu Glu Tyr Phe Ala Thr Ala Leu Phe Phe Ala Ser His Arg Trp
165 170 175
Gly Asp Gly Asn Gly Ile Tyr Asn Tyr Ser Ala Glu Ala Asn Asn Ile
180 185 190
Leu Asn Thr Met Leu His Lys Glu Asp Met Asn Gly Gly Val Val Asn
195 200 205
Gly Val Thr Asn Met Phe Asp Arg Glu His Lys Gln Val Val Phe Val
210 215 220
Pro Glu Gly Asp Asn Ala Ile Phe Thr Asp Pro Ser Tyr His Leu Pro
225 230 235 240
Ala Phe Tyr Glu Leu Trp Ser Arg Trp Ala Thr Gly Trp Asn Gly Gln
245 250 255
Gln Ala Val Asp Arg Lys Phe Trp Ala Glu Ala Ala Gln Val Ser Arg
260 265 270
Asp Tyr Phe Gln Lys Ala Thr His Pro Glu Thr Gly Leu Gly Ser Val
275 280 285
Trp Leu Lys Phe
290
<210> 132
<211> 387
<212> PRT
<213> Ralstonia metallidurans
<400> 132
Met Arg Arg Ala Met Ala Trp Leu Leu Arg Ala Thr Val Val Cys Thr
1 5 10 15
Leu Ala Gly Thr Val Leu Thr Ser Ala Ala Ala Thr Cys Ala Trp Pro
20 25 30
Asp Trp Asp Ala Phe Arg Arg Ser Thr Ile Ser Gln Asp Gly Arg Val
35 40 45
Ile Asp Asp Ser Thr Asp Gln Gln Val Thr Val Ser Glu Gly Gln Ser
50 55 60
Tyr Ala Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Ala Thr Phe Asp
65 70 75 80
Lys Leu Leu Thr Trp Thr Glu Asn Asn Leu Ala Gln Gly Asp Leu Thr
85 90 95
Ala His Leu Pro Ala Trp Ile Trp Gly Lys Arg Thr Glu Gly Glu His
100 105 110
Ala Gly Thr Trp Gly Val Ile Asp Ala Asn Pro Ala Ser Asp Ala Asp
115 120 125
Leu Trp Ile Ala Tyr Ser Leu Leu Glu Ala Gly Arg Leu Trp Asn Glu
130 135 140
Arg Arg Phe Thr Ala Leu Gly Thr Leu Leu Ala Arg Arg Ala Leu Arg
145 150 155 160
Glu Glu Thr Ala Val Leu Pro Gly Leu Gly Arg Thr Leu Leu Pro Gly
165 170 175
Pro Thr Gly Phe His Thr Ser Pro Asp Val Trp Arg Leu Asn Pro Ser
180 185 190
Tyr Val Pro Leu Gln Val Met Arg Arg Leu Ala Ala Met Pro Gly Glu
195 200 205
Asp Ser Ser Trp Lys Thr Leu Val Asp Thr Ser Ala Arg Leu Ile Leu
210 215 220
Asn Thr Ala Pro His Gly Phe Ser Pro Asp Trp Val Glu Tyr His Lys
225 230 235 240
Gly Arg Gly Phe Arg Pro Asp Thr Gln Thr Gln Ala Gly Gly Ser Tyr
245 250 255
Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met Ala Pro Arg Asp
260 265 270
Pro Leu Arg Thr Ser Val Leu Gln Ala Phe Arg Pro Met Ala Asp Tyr
275 280 285
Val Arg Lys Gln Gly Asp Pro Pro Glu Arg Val Asp Thr Gln Ser Gly
290 295 300
Thr Phe Gly Pro Asn Ser Gly Asn Gly Gly Phe Ser Ala Ala Val Ala
305 310 315 320
Pro Tyr Leu Ala Ala Leu Gly Gln Thr Asp Asp Ala Arg Ala Gln Ala
325 330 335
Gln Arg Ala Arg Asp Leu Ala Ala Gln Lys Pro Ala Gly Tyr Tyr Ser
340 345 350
Gln Val Leu Ala Leu Phe Gly Leu Gly His Leu Asp Gly His Phe Arg
355 360 365
Phe Glu Ala Asp Gly Thr Leu Val Pro Ala Trp Lys Ser Thr Cys Pro
370 375 380
Ala Thr Arg
385
<210> 133
<211> 344
<212> PRT
<213> Candidatus Kuenenia stuttgartiensis
<400> 133
Met His Lys Lys Leu Ser Cys Ile Val Ile Cys Val Phe Phe Met Leu
1 5 10 15
Gly Met Cys Lys Met Thr Tyr Gly Arg Gln Pro Ala Val Trp Glu Ala
20 25 30
Phe Lys Thr Asn Phe Ile Gly Gln Asp Gly Arg Val Val Asp His Tyr
35 40 45
Asn Gly Arg Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu Leu
50 55 60
Ala Val Lys Tyr Asp Asp Lys Ala Val Phe Asp Lys Leu Leu Gln Trp
65 70 75 80
Thr Gln Asp Asn Ile Ala Val Arg Asn Asp His Leu Phe Ala Trp Lys
85 90 95
Trp Gly Glu Arg Thr Ser Gly Val Trp Gly Val Val Asp Tyr Asn Asn
100 105 110
Ala Thr Asp Gly Asp Ile Leu Ile Ala Tyr Ala Leu Ile Lys Ala Asp
115 120 125
Glu Glu Trp Pro Asn Asn Asn Tyr Lys Asn Thr Ala Leu Lys Ile Val
130 135 140
Arg Ser Val Arg Glu Asn Leu Ala Val Glu Trp Asn Gly His Gln Leu
145 150 155 160
Leu Leu Pro Gly Tyr His Gly Phe Ile Arg Glu Asn Asp Arg Phe Ile
165 170 175
Val Asn Pro Ser Tyr Val Ile Phe Ser Ala Tyr Arg Ala Phe Ala Lys
180 185 190
Val Asp Ala Glu Ala Phe Trp Gln Lys Val Tyr Lys Asp Ser Met Leu
195 200 205
Leu Val Ala Lys Ser Cys Phe Gly Ser Leu Lys Met Pro Ala Asp Trp
210 215 220
Val Met Leu Glu Gly Arg Asp Ile Asp Val Cys Asn Glu Arg Lys Pro
225 230 235 240
Tyr Phe Gly Tyr Glu Ala Ile Arg Val Phe Leu Tyr Leu Ser Trp Glu
245 250 255
Glu Asn Pro Leu Phe Pro Glu Gly Leu Ala Lys Met Leu Asp Ile Tyr
260 265 270
Asn Lys Leu Gly Phe Ile Pro Leu Trp Val Asp Val Ala Ser Asp Ser
275 280 285
Ile Ser Leu Gln Asn Ala Pro Pro Gly Phe Tyr Ala Val Phe Ala Arg
290 295 300
Ala Ala Glu Lys Met Gly Glu Lys Thr Leu Ser Lys Lys Leu Phe Lys
305 310 315 320
Glu Ala Ser Glu Arg Leu Ala Tyr Asp Lys Lys Asp Tyr Tyr Ser Tyr
325 330 335
Cys Leu Tyr Leu Leu Ala Glu Glu
340
<210> 134
<211> 368
<212> PRT
<213> 解藻朊酸弧菌
<400> 134
Met Lys Lys Leu Ile Ser Phe Leu Met Leu Phe Val Ser Pro Tyr Leu
1 5 10 15
Trp Ala Asn Thr Cys Glu Trp Pro Gln Trp Glu Thr Phe Lys Ser Val
20 25 30
Tyr Met Glu Gln Gly Arg Val Val Asp Gly Ser Asp Glu Arg Met Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala Leu Val Ala
50 55 60
Asn Asp Pro Lys Thr Phe Asp Gln Val Leu Lys Trp Thr Gln Ser His
65 70 75 80
Leu Ala Gly Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Arg Lys Glu Asn Gly Gln Phe Gly Val Leu Asp Ser Asn Pro Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ser Leu Val Glu Ala Gly Arg Leu
115 120 125
Trp Asn Asn Tyr Tyr Tyr Gln Thr Leu Gly His Leu Ile Ala Ser Arg
130 135 140
Ile Leu Arg Glu Glu Thr Val Asn Ile Ala Gly Val Gly Thr Val Leu
145 150 155 160
Leu Pro Ala Pro Thr Gly Phe Asp Ala Asp Gly Gln Tyr Arg Val Asn
165 170 175
Pro Ser Tyr Val Pro Leu Gln Leu Ile Ala Arg Met Gln Ser Leu Tyr
180 185 190
Pro Gln Tyr Asn Trp Asp Ser Met Tyr Lys Ala Ser Val His Met Leu
195 200 205
Glu Lys Thr Met Pro Ala Gly Phe Ser Pro Asp Trp Ala Val Leu Arg
210 215 220
Asn Gly Arg Tyr Ser Ser Asp Val Val Thr Gly Pro Met Gly Ser Tyr
225 230 235 240
Asn Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Asn Asp Gln Val
245 250 255
Ser Glu Lys Ala Val Leu Val Gln Lys Met Gln Pro Phe Val Ala Ala
260 265 270
Thr Lys Ala Leu Gly Ala Pro Ala Arg Glu Val Asn Thr Glu Thr Gly
275 280 285
Lys Tyr Thr Gln Ala Gly Ser Ala Gly Phe Ser Ala Ala Ala Leu Pro
290 295 300
Leu Leu Ala Ala Ser Gly Glu Ser Thr Leu Leu Glu Thr Gln Phe Arg
305 310 315 320
Arg Ala Gln Asn Glu Leu Val Val Asp Lys Asn Asp His Tyr Tyr Asp
325 330 335
Asn Val Leu Ser Leu Phe Gly Leu Gly Trp His Glu Glu Arg Tyr Arg
340 345 350
Phe Gly Val Gln Gly Glu Leu Leu Pro Val Trp Ser Glu Arg Cys Gln
355 360 365
<210> 135
<211> 364
<212> PRT
<213> Aurantimonas sp. SI85-9A1
<400> 135
Met Arg Ser Ile Leu Thr Leu Cys Ala Leu Ala Ser Leu Val Pro Ala
1 5 10 15
Met Thr Tyr Pro Pro Ala Ala His Ala Thr Pro Val Thr Ala Thr Ser
20 25 30
Ala Ala Pro Glu Val Leu Pro Ala Asp Tyr Ile Val Gly Ser Trp Gln
35 40 45
Leu Tyr Arg Ser Leu Phe Met Ser Ala Glu Gly Arg Ile Ile Asp Arg
50 55 60
Glu Asn Gly Ser Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu
65 70 75 80
Ile Ala Val Ser Ala Gly Asp Arg Gln Ser Phe Asp Ala Leu Trp Asp
85 90 95
Trp Thr Glu Arg Glu Leu Met Val Arg Asp Asp Asn Leu Ala Ala Trp
100 105 110
Lys Trp Asp Pro Ala Ala Ser Pro His Val Thr Asp Pro Asn Asn Ala
115 120 125
Thr Asp Gly Asp Ile Leu Ile Ala Trp Ala Leu Leu Arg Ala Trp Lys
130 135 140
Leu Trp Gly Val Arg Asp His Leu Arg Glu Ala Arg Ala Ile Val Gly
145 150 155 160
Asp Val Val Ala Leu Ala Thr Thr Asp Ser Arg Tyr Gly Thr Val Leu
165 170 175
Leu Pro Gly Val Gln Gly Phe Thr Val Glu Asp Ser Ala Asp Gly Pro
180 185 190
Val Val Asn Met Ser Tyr Trp Val Phe Pro Ala Ile Ser Glu Leu Gly
195 200 205
Glu Ala Met Pro Glu Phe Lys Ala Leu Asn Leu Ala Ala Ser Gly Phe
210 215 220
Ala Leu Leu His Ala Ile Asp Ala Arg Pro Glu Lys Ile Pro Ser Asp
225 230 235 240
Trp Thr Gly Ile Gly Asp Glu Val Pro His Pro Ala Thr Ala Phe Ala
245 250 255
Pro Thr Phe Gly Tyr Asn Ala Val Arg Ile Pro Leu Tyr Ile Ala Trp
260 265 270
Gln Thr Asn Thr Glu Pro Gln Leu Leu Glu Ser Leu Gln Arg Ala Trp
275 280 285
Gly Asp Glu Gly Arg Ser Gly Leu Ala Val Leu Asp Val Thr Thr Gly
290 295 300
Thr Pro Ile Glu Pro Met Val Ser Pro Gly Tyr Asp Ala Ile Leu Asp
305 310 315 320
Val Ile Ala Cys Ser Leu Gly Gln Ser Asn Ser Ala Arg Arg Ala Lys
325 330 335
Ala Phe Glu Pro Ser Thr Tyr Tyr Ala Ser Thr Leu His Ile Leu Ser
340 345 350
Leu Met Ala Leu Ser Glu Arg Tyr Ser Glu Cys Phe
355 360
<210> 136
<211> 369
<212> PRT
<213> Photobacterium profundum
<400> 136
Met Lys Lys Leu Val Leu Ile Ile Gly Phe Ile Phe Ser Tyr Gln Val
1 5 10 15
Asn Ala Ser Thr Cys Ser Trp Pro Glu Trp Glu Gln Phe Lys Ser Thr
20 25 30
Tyr Ile Gln Asn Gly Arg Val Ile Asp Gly Ser Asp Ala Arg Lys Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe Ala Leu Val Ala
50 55 60
Asn Asp Pro Thr Ala Phe Lys Ala Met Leu Asn Trp Thr Glu Thr Tyr
65 70 75 80
Leu Ala Asp Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Lys Asp Leu Asn Thr Phe Gly Val Leu Asp Asn Asn Pro Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ser Leu Ile Glu Ala Gly Arg Leu
115 120 125
Trp Gly Glu Tyr Tyr Tyr Glu Ser Leu Gly Tyr Leu Leu Ala Ser Arg
130 135 140
Ile Leu Arg Glu Glu Thr Arg Glu Val Lys Gly Ile Gly Thr Val Leu
145 150 155 160
Leu Pro Gly Lys Lys Gly Phe Asp Asn Gly Lys Gly Gln Leu Arg Leu
165 170 175
Asn Pro Ser Tyr Val Pro Leu Phe Leu Ile Lys Asn Met Val Ser His
180 185 190
Tyr Pro Asn His Gln Trp Gln Ser Leu Tyr Asn Ser Ser Tyr Thr Met
195 200 205
Leu Glu Lys Thr Met Pro Lys Gly Phe Ser Pro Asp Trp Val Thr Leu
210 215 220
Ser Asn Ala Thr Phe Tyr Ala Asp Ala Glu Thr Gly Pro Ile Gly Ser
225 230 235 240
Tyr Asn Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Asn Asp Lys
245 250 255
Thr Glu Glu Lys Gln Thr Leu Leu Ser Met Met Ser Pro Met Val Asn
260 265 270
Ala Ile Lys Gln Leu Gln Ala Pro Pro Arg Ser Val Asn Thr Glu Thr
275 280 285
Gly Thr Phe Lys Gly Gly Gly Ser Ala Gly Phe Ser Ala Ala Leu Leu
290 295 300
Pro Leu Leu Gln Ser Leu Gly Glu Lys Glu Leu Ala Ile Glu Gln Ala
305 310 315 320
Lys Ile Ile Ser Ile Ser Leu Gln Ser Asn Ser Asn Asp Tyr Tyr Tyr
325 330 335
Asp Asn Val Leu Ala Leu Phe Gly Met Gly Trp Tyr Gln Gly Lys Tyr
340 345 350
Gln Phe Gly Val Asn Gly Asp Leu Gln Pro Ser Trp Val Glu Gln Cys
355 360 365
Gln
<210> 137
<211> 414
<212> PRT
<213> 溶杆菌属菌种IB-9374
<400> 137
Met His His Ala Thr Ala Leu Pro Arg Arg Gly Leu Lys Leu Leu Ser
1 5 10 15
Leu Val Leu Ala Thr Ala Ala Ala Leu Gly Ala Ser Leu Ala Ala Gln
20 25 30
Ala Ala Pro Asn Tyr Pro Phe Gly Ser His Arg Gln Ala Tyr Val Ser
35 40 45
Gly Thr Leu Ser Pro Ser Val Gly Arg Ala Ala Ala Asp Gln Ser Thr
50 55 60
Ala Ser Phe Tyr Arg Ala Trp Lys Gln Arg Tyr Leu Val Ala Gly Cys
65 70 75 80
Lys Ala Gly Glu Tyr Arg Val Lys Ala Ser Thr Asp Thr Ala Tyr Val
85 90 95
Val Ser Glu Gly Gln Gly Tyr Gly Met Leu Ile Thr Val Met Met Ala
100 105 110
Gly Asn Asp Pro Asp Ala Gln Ala Leu Phe Asp Gly Leu His Arg Tyr
115 120 125
Asn Arg Gly His Pro Ser Ser Gly Asn Pro Ala Leu Leu Ala Trp Ala
130 135 140
Gln Asp Ala Asn Cys Lys Asn Val Ala Gly Pro Asp Ser Ala Thr Asp
145 150 155 160
Gly Asp Leu Asp Ile Ala Tyr Ala Leu Leu Leu Ala Asp Leu Gln Trp
165 170 175
Gly Ser Ala Gly Ser Ile Asn Tyr Leu Ser Glu Ala Arg Lys Val Ile
180 185 190
Ala Ala Ile Arg Ser Ser Asn Val Asn Ala Asn Thr Lys Leu Val Thr
195 200 205
Leu Gly Asp Trp Val Ser Ser Ser Glu Pro Asn Tyr Tyr Asn Ser Thr
210 215 220
Arg Ser Ser Asp Trp Met Leu Gly His Phe Arg Gly Phe Ala Ser Lys
225 230 235 240
Leu Gly Asp Ser Tyr Trp Thr Gly Val Leu Asp Ala His Gln Thr Leu
245 250 255
Ile Gly Lys Met Gln Ser Ser Tyr Ala Pro Asn Thr Gly Leu Leu Pro
260 265 270
Asp Phe Ile Val Asn Thr Asn Thr Thr Ala Lys Pro Ala Pro Ala Asn
275 280 285
Phe Leu Glu Ser Glu Tyr Asp Gly Ala Tyr Ser Trp Asn Ala Gly Arg
290 295 300
Val Pro Trp Arg Ile Gly Ile Asp Ala Ala Val Ser Gly Asp Ser Arg
305 310 315 320
Ser Arg Asn Ala Ala Arg Lys Leu Ser Gln Trp Ile Lys Val Lys Ala
325 330 335
Gly Asn Asn Ala Asn Asn Ile Arg Ser Gly Tyr Lys Leu Asp Gly Thr
340 345 350
Val Thr Glu Asn Tyr Asn Ser Thr Phe Phe Thr Ala Pro Phe Ala Val
355 360 365
Ala Ala Thr Val Asp Thr Asp Gln Ala Trp Leu Asp Lys Leu Trp Thr
370 375 380
Tyr Leu Ala Asn Gly Ser Thr Ser Asp Tyr Tyr Gly Asp Ser Val Lys
385 390 395 400
Leu Leu Ser Met Leu Ala Val Thr Asn Asn Trp Leu Lys Pro
405 410
<210> 138
<211> 398
<212> PRT
<213> 鸟博德特氏菌
<400> 138
Met Met Leu Arg Leu Ala Ala Leu Arg Leu Ala Ala Leu Arg Leu Met
1 5 10 15
Ser Leu Val Leu Ala Leu Ile Ala Ala Pro Ala Ser Ala Ala Ala Val
20 25 30
Cys Gln Ala Ala Ser Trp Pro Gln Trp Gln Thr Phe Ala Gln His Phe
35 40 45
Val Gln Ala Asp Gly Arg Val Leu Asp Ala Ser Thr Pro Arg Gln Ile
50 55 60
Ser Thr Ser Glu Gly Gln Ser Tyr Ala Met Phe Phe Ala Leu Val Ala
65 70 75 80
Asn Asp Arg Ala Ala Phe Glu Arg Leu Trp Arg Trp Ser Val Ala Asn
85 90 95
Leu Ala Asp Ala Asp Ala Thr Arg Thr Leu Pro Ala Trp Ala Trp Gly
100 105 110
Lys Arg Asp Asp Gly Ser Trp Gly Val Leu Asp Arg Asn Ala Ala Ser
115 120 125
Asp Ala Asp Leu Trp Phe Thr Tyr Ala Leu Leu Glu Ala Gly Arg Leu
130 135 140
Trp Arg Arg Pro Asp Tyr Thr Arg Glu Gly Leu Ala Leu Leu Asn Leu
145 150 155 160
Ile Gln Asn Gln Glu Leu Ala Asp Leu Pro Asp Phe Gly Pro Met Leu
165 170 175
Leu Pro Ala Pro Phe Gly Phe Val Gln Pro Asp Gly Ile Trp Arg Leu
180 185 190
Asn Pro Ser Tyr Leu Pro Ile Pro Val Leu Arg Arg Leu Ala Leu Ala
195 200 205
Ala Pro Gly Gly Pro Trp Thr Lys Ile Ala Ala Asn Thr Val Arg Leu
210 215 220
Ile Gln Ala Thr Thr Pro Lys Gly Phe Ala Ala Asp Trp Val Ala Tyr
225 230 235 240
Gln Ser Thr Ala Gln Ile Ala Gly Gln Phe Val Ala Asp Pro Val His
245 250 255
Gly Tyr Arg Gly Ser Tyr Asp Ala Ile Arg Thr Tyr Met Trp Ala Gly
260 265 270
Met Thr Pro Ala Ala Asp Pro Ala Ala Gln Pro Leu Arg Ala Ala Leu
275 280 285
Tyr Gly Met Ala Ala Ala Thr Leu Ala Ala Gly Val Pro Pro Glu Ser
290 295 300
Val Gln Thr Asp Thr Gly Val Val Gln Gly Ser Gly Pro Phe Gly Phe
305 310 315 320
Ser Ala Ala Leu Leu Pro Tyr Leu Glu Ala Glu Gly Arg Asp Ala Glu
325 330 335
Leu Arg Thr Gln Leu Ala Arg Val Arg Ala Ala Trp Thr Gln Ser Leu
340 345 350
Glu Pro Gly Asn Leu Ala Leu Arg Gln Pro Ser Tyr Tyr Asp Tyr Val
355 360 365
Leu Ser Leu Phe Gly Thr Gly Trp Tyr Glu Gln Arg Tyr Arg Phe Gly
370 375 380
Thr Thr Gly Arg Leu Ser Leu Glu Trp Glu Lys Lys Cys Pro
385 390 395
<210> 139
<211> 555
<212> PRT
<213> Hahella chejuensis
<400> 139
Met Met Pro Asp Thr Phe Arg Ile Ala Pro Leu Ala Ala Arg Val Ile
1 5 10 15
Leu Leu Ser Gly Leu Ala Leu Gly Ala Gln Val Ala Phe Ala Ser Cys
20 25 30
Ala Asp Ser Thr Ala Thr Thr Ala Ser Ala Ala Ala Asp Ala Val Cys
35 40 45
Gln Pro Ala Leu Glu Gln Gln Ala Gln Ala Ala Arg Pro Phe Pro Gln
50 55 60
Ala Leu Glu Phe Ala Gly Lys Ile Lys Pro Asp His Val Ser Gln Thr
65 70 75 80
Gln Met Asn Ala Lys Val Ala Gln Phe Tyr Asp Tyr Trp Lys Ser Ala
85 90 95
Tyr Val Lys Pro Ser Asn Gly Asn Thr Pro Gly Gly Gly Tyr Tyr Val
100 105 110
Asn Met Lys Gly Thr Gly Gly Asp Gly Asn Glu Ile Thr Thr Ser Glu
115 120 125
Ala His Gly Tyr Gly Met Met Leu Phe Ala Leu Met Ala Gly His Asp
130 135 140
Ser Glu Ala Lys Gln Tyr Phe Asp Gly Met Tyr Asn Met Tyr Asp Lys
145 150 155 160
His Arg Ser Thr Leu Asn Asn His Leu Met Ser Trp Val Ile Asp Ala
165 170 175
Ser Glu Asn Thr Ser Lys Asp Ser Asp Ser Ala Thr Asp Gly Asp Met
180 185 190
Asp Ile Ala Tyr Ala Leu Leu Leu Ala His Tyr Gln Trp Gly Ser Asp
195 200 205
Gly Ala Ile Asn Tyr Leu Gln Gln Ala Lys Arg Ile Ile Asn Asn Gly
210 215 220
Leu Lys Gly Asp Asp Val His Arg Ser Ser Lys Arg Thr Met Leu Gly
225 230 235 240
Asp Trp Asp Asp Asn Gln Trp Thr Thr Arg Ser Ser Asp Trp Met Thr
245 250 255
Asp His Met His Ala Tyr Gln Gln Ala Thr Gly Asp Ala Phe Trp Gly
260 265 270
Glu Ala Ala Asp Thr Ala Tyr Gly Ile Ile Asp Lys Met Ile Lys Asn
275 280 285
Tyr Ala Ser Ala Thr Gly Leu Met Ser Asp Phe Val Ile Asp Ser Asp
290 295 300
Pro Arg Pro Ala Pro Pro Asn Phe Leu Glu Ala Asp Thr Asp Asp Asp
305 310 315 320
Phe Ser Trp Asn Ala Cys Arg Tyr Pro Leu Arg Ile Ala Ile Asp Ala
325 330 335
Ala His Phe Gly Asp Ser Arg Ala Ser Ala Ala Met Asn Lys Leu Met
340 345 350
Gln Trp Ala Ile Asn Ala Thr Gly Gly Asn Pro Gly Ser Val Met Ala
355 360 365
Gly Tyr Lys Leu Asn Gly Glu Pro Leu Val Gly Tyr Ser Ala Met Ala
370 375 380
Phe Thr Ala Pro Met Val Ala Ala Ala Ser Val Thr Gly His Gln Ser
385 390 395 400
Phe Leu Asn Gln Gly Trp Asp Leu Ile Ser Gly Asn Arg Ser Asp Tyr
405 410 415
Tyr Ser Asp Ser Ile Asn Leu Leu Ser Met Leu Phe Ile Ser Gly Asn
420 425 430
Trp Trp Ser Pro Val Glu Gly Gly Gly Asp Asp Gly Asp Asp Gly Gly
435 440 445
Asp Gly Gly Asp Asp Gly Asp Asp Gly Asp Asn Gly Gly Asp Asp Gly
450 455 460
Ser Asp Asp Gly Gly Asp Gln Asp Asn Pro Ala Gln Thr Thr Leu Ser
465 470 475 480
Lys Thr Asp Asp Trp Gly Gly Gly Tyr Cys Ala Asn Val Arg Val Phe
485 490 495
Asn Ser Ser Asp Ser Asp Leu Val Trp Ser Val Ser Val Pro Ile Glu
500 505 510
Gly Gln Ala Tyr Thr Val Trp Ser Ala Asn Trp Ser Gln Gln Gly Asp
515 520 525
Ala Leu Asn Ala Ser Gly Val His Trp Asn Arg Glu Leu Gly Pro Gln
530 535 540
Glu Ser Thr Glu Phe Gly Phe Cys Ala Asn Arg
545 550 555
<210> 140
<211> 398
<212> PRT
<213> Chlorobium chlorochromatii
<400> 140
Met Arg Arg Phe Trp Thr Phe Leu Leu Ile Ser Cys Ala Pro Phe Phe
1 5 10 15
Leu Ser Ser Cys Ser Ser Val Pro Gln Asn Pro Glu Lys Ile Ser Thr
20 25 30
Glu Ala Trp His Tyr Tyr Arg Asp Thr Phe Ile Lys Asn Gly Arg Val
35 40 45
Ile Arg Pro Gln Asn Asn Asn Asp Thr Val Ser Glu Gly Gln Ala Tyr
50 55 60
Thr Met Val Arg Ala Val Leu Met Lys Asp Arg Lys Thr Phe Asp Glu
65 70 75 80
Cys Leu Ala Trp Ser Glu Lys Val Leu Ser Arg Lys Asn Ser Asp Gly
85 90 95
Asp Tyr Leu Leu Ala Trp His Tyr Arg Asp Gly Lys Val Thr Asp Thr
100 105 110
Thr Ala Ala Ser Asp Ala Asp Ile Asp Tyr Ala Phe Ser Leu Ile Val
115 120 125
Ala Ser Lys Ile Trp Gln Ala Pro Arg Tyr Leu Glu Leu Ala Lys Glu
130 135 140
Val Leu Ala Ser Ile Leu Glu Ala Glu Thr Thr Arg His Gln Gly Arg
145 150 155 160
Leu Tyr Leu Leu Pro Trp Pro Ala Asn Lys Asn Lys Pro Gly Asp Leu
165 170 175
Leu Ala Gln Asn Leu Ser Tyr Tyr Ala Pro Ser His Phe Lys Leu Phe
180 185 190
Tyr Glu Thr Thr Ser Asp Pro Arg Trp Leu Glu Leu Val Asp Thr Thr
195 200 205
Tyr Tyr Leu Leu Gly Arg Leu Leu His Pro Gly Glu Leu Pro Glu Gly
210 215 220
Pro Ile Val Pro Asp Trp Ile Ala Ile Asn Asp Ala Gly Ala Phe Val
225 230 235 240
His Leu Pro Gly Lys Asp Val Arg Tyr Gly Trp Asp Ala Val Arg Val
245 250 255
Pro Met Arg Ile Ala Ala Asp Tyr His Leu Tyr Gly Asp Lys Arg Ala
260 265 270
Phe Glu Val Leu Ser Trp Leu Ala Val Ser Phe Glu Glu Glu Phe Arg
275 280 285
Gln Gln Ser Lys Phe Leu Leu Gln Arg Asp Ser Thr Leu Gln Val Arg
290 295 300
Asn Asn Ala Leu Phe Tyr Ser Ala Met Tyr Ala Ser Leu Glu Ala Thr
305 310 315 320
Glu Ser Pro Ser Ala Pro Lys Leu Leu Gln Arg Ile Arg Lys Phe Ile
325 330 335
Arg Gln Glu Lys Gln Gly Leu Phe Tyr Asn His Pro Asp Asp Tyr Tyr
340 345 350
Ile Asn Ser Leu Cys Trp Ile Thr Glu Tyr Tyr Glu Gln Asn Lys Lys
355 360 365
His Leu Gln Ala Arg Ser Lys Lys Val Ala Leu Pro Leu Gln Thr His
370 375 380
Glu Ser Thr Ala Asn Thr Ala Ser Leu Ser Leu Gln Ala Pro
385 390 395
<210> 141
<211> 376
<212> PRT
<213> 微黄暗网菌
<400> 141
Met Leu Ile Val Gly Leu Leu Ala Leu Ala Thr Val Trp Leu Val Ser
1 5 10 15
Cys Trp Pro Ala Glu Thr Asp Asp Ala Ile Val Leu Arg Lys Ser Trp
20 25 30
Ala Gly Tyr Leu His Thr Phe Val Gln Asp Gly Arg Val Val Arg Pro
35 40 45
Arg Asn Gly Phe Asp Thr Val Ser Glu Gly Gln Ala Tyr Ala Met Ile
50 55 60
Arg Ala Val Thr Ala Ser Asp Arg Thr Ser Phe Asp Ala Ile Leu Leu
65 70 75 80
Trp Thr Glu Lys Asn Leu Ser Arg Arg Glu Gln Ser Gly Asp Asn Leu
85 90 95
Leu Ala Trp His Tyr Ala Asp Gly Arg Val Val Asp Trp Gln Ala Ala
100 105 110
Ser Asp Ala Asp Ile Asp Tyr Ala Tyr Ser Leu Leu Leu Ala Gly Arg
115 120 125
Lys Trp Gly Asp Pro Ser Tyr Ala Arg Leu Ala Arg Lys Val Leu Ala
130 135 140
Asp Ile Leu Arg Leu Glu Thr Ile Ser Tyr Glu Gly Arg Leu Arg Leu
145 150 155 160
Leu Pro Trp Asn Arg Lys Pro Thr Asp Gly Asn Gly Tyr Val Val Gln
165 170 175
Asn Pro Ser Tyr Tyr Ser Pro Ala Gln Phe Lys Leu Phe Phe Ala Glu
180 185 190
Thr Gly Asp Gly Arg Trp Leu Glu Leu Ala Ala Thr Gly Tyr Asp Leu
195 200 205
Leu Asp Arg Leu Gln Glu Pro Ala Gly Gly Gly Ala Phe Leu Val Pro
210 215 220
Asp Trp Cys Arg Ile Gly Glu Gly Gly Asp Leu Arg Glu Leu Glu Gly
225 230 235 240
Tyr Ser Ser Leu Tyr Gly Trp Asp Ala Leu Arg Val Pro Met Arg Ile
245 250 255
Ala Leu Asp His Ala Leu Phe His Glu Pro Arg Ala Ala Arg Val Leu
260 265 270
Gly Arg Phe Ala Glu Phe Tyr Thr Ser Glu Phe Lys Arg Phe Gly His
275 280 285
Val His Ser Val Tyr Ser Thr Gly Gly Arg Ser Val Val Tyr Asp Glu
290 295 300
Asn Pro Leu Ser Tyr Ala Ala Ala Tyr Ala Ala Leu Glu Ala Ser Gly
305 310 315 320
Ser Pro Leu Ala Glu Thr Ala Tyr Arg Arg Leu Gln Arg Phe Ser His
325 330 335
Leu Lys Lys Gly His Ile Tyr Tyr Leu Asp Arg Lys Asp Tyr Tyr Ala
340 345 350
Asn Ser Leu Ser Trp Leu Pro Ser Tyr Tyr Arg Leu Leu Leu Asn Ser
355 360 365
Arg Lys Ser Ser Leu Gln Asp Arg
370 375
<210> 142
<211> 393
<212> PRT
<213> Xanthomonas euvesicatoria
<400> 142
Met Ser Ala His Ala His Thr Gly Gly Met Thr Arg Arg Arg Leu Leu
1 5 10 15
His Ala Gly Ala Leu Ala Gly Val Ala Ala Leu Leu Pro Ala Ala Ala
20 25 30
Arg Ala Ala Pro Ser Gln Cys Gly Pro Trp Pro Leu Trp Ser Ala Phe
35 40 45
Val Asp Lys His Ile Gln Pro Asp Gly Arg Val Val Asp Phe Leu Asn
50 55 60
Pro Asp Gln Arg Ser Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe
65 70 75 80
Ala Leu Val Asn Asn Asp Gln Val Leu Phe Asp Lys Val Leu Ser Trp
85 90 95
Thr Arg His Asn Leu Cys Gly Gly Arg Pro Asp Leu His Leu Pro Ala
100 105 110
Trp Leu Trp Gly Arg Asp Gly Gly Ser Ala Trp Arg Val Leu Asp Ala
115 120 125
Asn Thr Ala Ser Asp Gly Glu Leu Trp Ile Ala Tyr Ala Leu Leu Glu
130 135 140
Ala Gly Arg Leu Trp Ser Arg Pro Gly Tyr Leu Lys Ala Gly Gln Gln
145 150 155 160
Met Leu Gln Leu Ile Arg Thr Gln Glu Val Ala Thr Leu Pro Gly Leu
165 170 175
Gly Pro Met Leu Leu Pro Gly Arg Thr Gly Phe Val Asp Asn Gly Arg
180 185 190
Trp Thr Leu Asn Pro Ser Tyr Leu Pro Ile Gln Val Leu Arg Arg Cys
195 200 205
Ala Asn Ala Asp Pro Lys Gly Pro Trp Ala Ala Ile Ala Ala Asn Ser
210 215 220
Ala Arg Val Leu Arg Asp Ser Ala Pro Val Gly Phe Ala Pro Asp Trp
225 230 235 240
Thr Val Trp Asp Gly Lys Thr Phe Asn Ala Asp Pro Lys Arg Gly Asn
245 250 255
Val Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Leu
260 265 270
Asp Ala Gly Glu Pro Leu Arg Ala Arg Leu Leu Lys Asp Leu Ser Gly
275 280 285
Pro Ala Asp Leu Leu Ala Ala Gln Gln Thr Pro Ala Glu Lys Ile Asp
290 295 300
Thr Ala Arg Gly Val Gly Thr Gly Ala Leu Pro Val Gly Phe Ser Ala
305 310 315 320
Ala Leu Leu Pro Tyr Leu Ser Ala Leu Gly Lys Pro Ala Leu Leu Lys
325 330 335
Ala Gln Ala Gln Arg Val Pro Ala Ala Thr Gln Pro Ala Ala Ala Ala
340 345 350
Leu Pro Tyr Phe Glu Arg Thr Leu Ala Leu Phe Gly Gln Gly Trp Leu
355 360 365
Glu Asn Arg Tyr Arg Phe Ala Ala Asp Gly Arg Leu Leu Pro Ala Trp
370 375 380
Arg Thr Pro Ala Cys Ala Ala Thr Thr
385 390
<210> 143
<211> 376
<212> PRT
<213> Pseudoalteromonas haloplanktis
<400> 143
Met Lys Leu Phe Ile Thr Lys Ala Phe Ile Met Leu Ile Leu Phe Thr
1 5 10 15
Pro Leu Ser His Ala Gln Gln Cys Ala Pro Trp Gln Gln Trp Gln Thr
20 25 30
Phe Lys Thr His Phe Met Ser Asp Asp Gly Arg Ile Ile Asp Leu Gly
35 40 45
Ser Glu Gln Asn Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe
50 55 60
Phe Ala Leu Ile Ala Asn Asp Lys Thr Ala Phe Asp Lys Val Leu Asn
65 70 75 80
Trp Thr Glu Glu His Leu Ala Glu Gly Asp Leu Ser Thr Arg Leu Pro
85 90 95
Ala Trp Leu Trp Gly Val Asn Lys Asn Asn Val Gly Asn Ile Leu Asp
100 105 110
Ser Asn Pro Ala Ser Asp Ser Asp Leu Trp Ile Ala Tyr Ser Leu Ser
115 120 125
Gln Ala Ala Ile Leu Trp Asp Asp Arg Arg Tyr Arg Ile Leu Ala Ala
130 135 140
Val Leu Ala Gln Arg Ile Met Arg Glu Glu Thr Ala Tyr Ile Lys Gly
145 150 155 160
Leu Gly Leu Ser Leu Leu Pro Ala Pro Ser Gly Phe Glu Phe Asp Asn
165 170 175
Lys Arg Tyr Lys Leu Asn Pro Ser Tyr Ser Pro Leu Phe Ile Tyr Gln
180 185 190
Gln Phe Ser Lys Leu Tyr Pro His Ser Pro Trp Gln Glu Leu His Asp
195 200 205
Gly Ser Ala Asn Leu Ile Leu Glu Thr Ser Lys Gln Gly Val Ser Pro
210 215 220
Asp Trp Ile Met Phe Asp Thr Asn Lys Gly Phe Tyr Phe Asp Lys Lys
225 230 235 240
Val Thr Asp Leu Gly Ser Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala
245 250 255
Ser Met Met Ala Glu Asp Ala Pro Tyr Lys Lys Gln Leu Val Thr Gln
260 265 270
Phe Ala Pro Phe Ile Lys Thr Ile Asn Lys Arg Gly Tyr Val Pro Leu
275 280 285
Asn Thr Tyr Ala Gln Thr Gly Asn Ser Asp Lys Arg Gly Pro Val Gly
290 295 300
Phe Asn Ala Ala Leu Leu Pro Leu Leu Ala Thr Ala Asn Asn Asp Asn
305 310 315 320
Asp Ala Val Thr Met Ser Ile Gln Gln Lys Leu Met Val Asp Lys Ser
325 330 335
Phe Ser Lys Ser Arg Tyr Tyr Asp Ser Val Leu Asn Leu Phe Gly Ser
340 345 350
Ser Thr Leu Asp Lys Arg Phe Ala Ile Leu Ala Asp Gly Thr Leu Gln
355 360 365
Pro Asn Trp Ser Asp Glu Cys Arg
370 375
<210> 144
<211> 392
<212> PRT
<213> Cupriavidus pinatubonensis
<400> 144
Met Trp Arg Ala Leu Pro Gly Leu Thr Arg Trp Leu Ala Ala Gly Ala
1 5 10 15
Leu Ala Cys Ala Cys Val Ala Ala Leu Pro Ala His Ala Ala Thr Cys
20 25 30
Gly Trp Ala Asp Trp Asp Thr Phe Lys Arg Thr Leu Ile Ser Ala Asp
35 40 45
Gly Arg Val Ile Asp Ala Ser Thr Asp Asp Lys Val Thr Val Ser Glu
50 55 60
Gly Gln Ala Tyr Gly Leu Phe Phe Ala Leu Val Ala Asn Asp Arg Thr
65 70 75 80
Thr Phe Asp Lys Leu Leu Ala Trp Thr Glu Asn Asn Leu Ala Gln Gly
85 90 95
Asp Leu Ile Ala His Leu Pro Ala Trp Ile Trp Gly Arg Ile Pro Ala
100 105 110
Asp Lys Ala Lys Asp Gly Lys Glu Thr Trp Gly Val Ile Asp Thr Asn
115 120 125
Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Thr Leu Leu Glu Ala
130 135 140
Gly Arg Leu Trp Asn Glu Arg Arg Phe Thr Ala Leu Gly Thr Leu Thr
145 150 155 160
Ala Arg Arg Ile Met Arg Glu Glu Thr Ala Ala Met Arg Gly Leu Gly
165 170 175
Arg Thr Val Leu Pro Gly Pro Val Gly Phe Ser Leu Ser Glu Gly Arg
180 185 190
Trp Arg Val Asn Pro Ser Tyr Val Pro Leu Gln Val Met Arg Arg Leu
195 200 205
Ala Val Leu Leu Ala Lys Glu Ser Gly Trp Asp Gln Leu Val Ala Ser
210 215 220
Ser Leu Lys Val Ile Leu Glu Thr Ala Pro Arg Gly Phe Ser Pro Asp
225 230 235 240
Trp Ala Glu Tyr Asp Asn Ser Arg Gly Phe Leu Pro Asp Thr Ala Thr
245 250 255
Lys Ala Glu Ser Ala Tyr Asn Ala Ile Arg Val Tyr Leu Trp Ala Gly
260 265 270
Thr Leu Ala Ala Asp Asp Pro Gln Arg Ala Arg Met Leu Gln Gln Phe
275 280 285
Met Pro Leu Ala Asp Tyr Val Ala Ala His Gly Tyr Ala Pro Glu Arg
290 295 300
Val Asp Thr Gln Thr Gly Gln Pro Gly Pro Asp Ser Gly Asn Ala Gly
305 310 315 320
Phe Ser Ala Ala Val Ala Pro Tyr Leu Ala Ala Leu Gly Arg Thr Asp
325 330 335
Gln Ala Arg Ala Gln Ala Gln Arg Thr Arg Glu Leu Thr Ala Arg Glu
340 345 350
Pro Leu Gly Tyr Tyr Ser Gln Ala Leu Ala Leu Phe Gly Leu Gly His
355 360 365
Leu Asp Gly Leu Tyr Arg Phe Ala Gly Asp Gly Ala Leu Leu Pro Ala
370 375 380
Trp Lys Thr Gly Cys Pro Ala Arg
385 390
<210> 145
<211> 361
<212> PRT
<213> Ustilago maydis
<400> 145
Met Glu Ala Lys Tyr Val Arg Trp Arg Asp Thr Tyr Leu Arg Ser Ser
1 5 10 15
Ala Val Gly Leu Tyr Cys Tyr Tyr Asn Gly Lys Gly Asp Asn Gly Asn
20 25 30
Ala Ile Thr Cys Ser Glu Ala His Gly Tyr Ala Met Leu Ile Ser Val
35 40 45
Leu His Arg Asn Arg Ser Asp Phe Asp Gly Leu Leu Ala Phe Phe Gln
50 55 60
Gly Phe Arg Asn Glu His Gly Leu Met Cys Trp Gln Ile Arg Ser Pro
65 70 75 80
His Gln Pro Gly Ser Asn Ala Ser Val Glu Pro Tyr Val Glu Glu Asp
85 90 95
Gly Arg Thr Ser Ala Thr Asp Gly Asp Ile Asp Ile Ala Ser Ser Leu
100 105 110
Tyr Leu Gly Ala Lys Thr Phe Gly Asp Gln Arg Tyr Lys Leu Glu Ala
115 120 125
Asp Arg Leu Ala Ser Ala Ile Leu Lys Tyr Thr Ile His Pro His Leu
130 135 140
Gly Thr Pro Leu Leu Gly Asp Trp Ala Asn Cys Asp Ser Ala Glu Ala
145 150 155 160
Lys Lys Leu Tyr Asp Ser Thr Arg Thr Ser Asp Phe Ile Leu Ser Ala
165 170 175
Phe Ala Leu Phe Ser Arg Met His Ser Asp Ala Ser Glu Arg Ser Arg
180 185 190
Trp Glu Trp Val Leu Gln Ser Thr Lys Gln Ala Ala Leu Cys Cys Gly
195 200 205
Gly Ser Thr Gly Leu Leu Pro Asp Phe Leu Thr Tyr Asn Thr Met Ser
210 215 220
Gln Thr Trp Gln Pro Ala Arg Ala Lys Leu Leu Glu Ser Glu His Asp
225 230 235 240
Gly Ala Leu Asn Trp Asn Ala Cys Arg Thr Pro Trp Arg Leu Ala His
245 250 255
Tyr Leu Ala Thr Thr Gly Asp Pro Ser Ile Val Pro Leu Leu Gln His
260 265 270
Ala His Arg Ser Val Arg Ser Ser Lys Ala Phe Asn Phe Pro Thr Ile
275 280 285
Pro Ala Gly Val Asp Ile Ala Ala Arg Glu Pro Lys Ala Leu Val Asp
290 295 300
Tyr Thr Asp Lys Ala Phe Ile Ala Pro Val Gly Tyr Leu Cys Tyr Val
305 310 315 320
Leu Gly Asp Thr Asp Gly His Ser Gln Cys Val Thr Ala Leu Asn His
325 330 335
Gln Glu Pro Gly Tyr Phe Gly Asp Ser Ile Asp Val Cys Ile Ala Glu
340 345 350
Gln Ala Gly His Ala His Glu Trp Phe
355 360
<210> 146
<211> 339
<212> PRT
<213> 运动发酵单胞菌
<400> 146
Met Thr Tyr Ser Arg Arg Phe Ile Leu Ser Thr Leu Val Ser Val Pro
1 5 10 15
Leu Leu Val Ala Cys Arg Lys Gly Lys Ala Ala Glu Ala Asp Asn Trp
20 25 30
Thr Leu Phe Lys Asn Arg Phe Phe Lys Asp Gly Arg Ile Ser Asp Ser
35 40 45
Gly Asn Gly Asn Ile Ser His Ser Glu Gly Gln Gly Tyr Gly Met Ile
50 55 60
Gln Ala Glu Ala Ala His Asp Lys Ala Thr Phe Asp Ala Leu Trp Gln
65 70 75 80
Trp Thr Lys Thr His Leu Met Arg Pro Asp Met Ala Leu Phe Ala Trp
85 90 95
Arg Phe Asp Pro Ser Gln Ser Asn Pro Val Ser Asp Gln Asn Asn Ala
100 105 110
Thr Asp Gly Asp Ile Leu Ile Ala Trp Ala Leu Leu Arg Ala Glu Lys
115 120 125
Arg Trp Pro Lys Asn Gly Tyr Gly Gln Asp Ser Glu Ala Ile Arg Lys
130 135 140
Ser Ile Gly Lys Lys Leu Val Leu Ser Gly Gly Gly Glu Thr Ile Leu
145 150 155 160
Leu Pro Gly Leu Gln Gly Phe Thr Gly Thr Asp Tyr Val Ile Leu Asn
165 170 175
Phe Ser Tyr Tyr Ile Trp Pro Ala Leu Lys Ala Phe Asn Glu Ala Asp
180 185 190
Asn Gly Ala Trp His Asn Val Ile Glu Ser Gly Lys Lys Leu Leu Ala
195 200 205
Lys Ala Lys Phe Gly Leu Pro Gln Leu Pro Thr Asp Trp Val Ala Phe
210 215 220
Lys Ser Asn Gly Asn Leu Glu Pro Ala Ala Asp Lys Gln Pro Tyr Phe
225 230 235 240
Gly Phe Asp Ala Val Arg Ile Pro Leu Tyr Leu Ile Trp Gly Gly Glu
245 250 255
Asp Ala Leu Ala Ala Pro Phe Ala Ile Tyr Trp Asn Ser Tyr Leu Ser
260 265 270
His Asn Gln Pro Val Pro Ala Trp Val Asp Val Asn Ser Gln Ala Ile
275 280 285
Ala Pro Tyr Pro Leu Ser Lys Gly Gly Met Ala Ile Leu Asp Leu Ala
290 295 300
Met Asn Lys Pro Ile Thr Ala Lys Ile Ala Asp Gln Asp Asp Tyr Tyr
305 310 315 320
Ser Ser Ala Leu Leu Ala Leu Ser Glu Ile Ala Ala Lys Glu Arg Pro
325 330 335
His Asn Arg
<210> 147
<211> 371
<212> PRT
<213> 胡萝卜软腐欧文氏菌
<400> 147
Met Pro Arg Val Leu Arg Tyr Leu Ile Leu Thr Leu Leu Trp Leu Trp
1 5 10 15
Ala Ser Leu Ala Thr Ala Ala Val Cys Asp Trp Pro Ala Trp Glu Gln
20 25 30
Tyr Lys Gln His Tyr Ile Ser Glu Gln Gly Arg Val Ile Asp Thr Ser
35 40 45
Thr Pro Asn Lys Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Met Phe
50 55 60
Phe Ala Leu Val Ala Asn Asp Arg Ala Met Phe Asp Arg Leu Leu Lys
65 70 75 80
Trp Thr Glu Asp Asn Leu Ser Ala Gly Asp Leu Arg Ala Asn Leu Pro
85 90 95
Ala Trp Leu Trp Gly Glu Ser Lys Asp Lys Gln Trp Ala Val Leu Asp
100 105 110
Pro Asn Ser Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Asn Leu Leu
115 120 125
Glu Ala Gly Arg Leu Trp Lys Glu Ala Arg Tyr Gln Thr Leu Gly Thr
130 135 140
Thr Leu Leu Ala Arg Ile Ala Lys Glu Glu Val Val Asn Ile Pro Gly
145 150 155 160
Leu Gly Val Met Leu Leu Pro Gly Lys Val Gly Phe Ala Glu Lys Glu
165 170 175
Ser Trp Arg Val Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala Arg
180 185 190
Phe Ala Pro Leu Gly Glu Thr Trp Lys Ser Met Gln Arg Thr Thr Gln
195 200 205
Arg Leu Leu Leu Glu Thr Ala Pro Lys Gly Phe Ser Pro Asp Trp Val
210 215 220
Ile Trp Gln Lys Gly Lys Gly Trp Gln Pro Asp Thr Thr Lys Pro Asn
225 230 235 240
Ile Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly Met Met
245 250 255
Ala Asp Ser Ser Arg Gly Lys Ala Asp Leu Leu Lys Gln Phe Gln Pro
260 265 270
Met Ile Gln Gln Thr Leu Gln Gln Gly Leu Pro Pro Glu Lys Ala Asp
275 280 285
Thr Ala Thr Gly Val Val Thr Gly Gln Gly Pro Val Gly Phe Ser Ala
290 295 300
Ser Leu Leu Pro Ile Leu Ser Arg Gln Pro Asp Ala Leu Ala Ala Gln
305 310 315 320
Arg Gln Arg Leu Ala Ala Asn Pro Pro Gly Asp Asp Ala Tyr Phe Ser
325 330 335
Ala Ser Leu Thr Leu Phe Gly Gln Gly Trp Asp Glu Lys Arg Tyr Arg
340 345 350
Phe Thr Ser Gln Gly Gln Leu Leu Pro Ser Arg Gly Ser Gln Cys Thr
355 360 365
Thr Thr Pro
370
<210> 148
<211> 369
<212> PRT
<213> Photobacterium profundum
<400> 148
Met Lys Lys Leu Val Leu Ile Ile Gly Phe Ile Leu Ser Tyr Gln Val
1 5 10 15
Asn Ala Ser Thr Cys Ser Trp Pro Glu Trp Glu Gln Phe Lys Ser Thr
20 25 30
Tyr Ile Gln Ser Gly Arg Val Ile Asp Gly Ser Asp Thr Arg Gln Ile
35 40 45
Thr Thr Ser Glu Gly Gln Ser Tyr Gly Leu Phe Phe Ala Val Val Ala
50 55 60
Asn Asp Pro Thr Ala Phe Lys Ala Val Leu Asn Trp Thr Glu Thr His
65 70 75 80
Leu Ser Gly Gly Asp Leu Thr Ala Arg Leu Pro Ala Trp Leu Trp Gly
85 90 95
Lys Lys Asp Phe Asn Thr Phe Gly Val Leu Asp Asn Asn Pro Ala Ser
100 105 110
Asp Ser Asp Leu Trp Ile Ala Tyr Ser Leu Ile Glu Ala Gly Arg Leu
115 120 125
Trp Gly Glu Tyr Tyr Tyr Glu Ser Leu Gly Tyr Leu Leu Ala Ser Arg
130 135 140
Ile Ile Arg Glu Glu Thr Arg Glu Val Glu Gly Ile Gly Thr Val Leu
145 150 155 160
Leu Pro Gly Lys Lys Gly Phe Asp Asn Gly Lys Gly Gln Leu Arg Leu
165 170 175
Asn Pro Ser Tyr Val Pro Leu Phe Leu Ile Lys Asn Met Ala Ser His
180 185 190
Tyr Pro Asn Asp Gln Trp Gln Ser Leu Tyr Asn Ser Ser Tyr Thr Met
195 200 205
Leu Glu Lys Thr Met Pro Lys Gly Phe Ser Pro Asp Trp Val Thr Leu
210 215 220
Ser Asn Ala Thr Phe Tyr Ala Asp Ala Glu Thr Gly Pro Ile Gly Ser
225 230 235 240
Tyr Asn Ala Ile Arg Thr Tyr Leu Trp Ala Gly Met Leu Asn Asp Lys
245 250 255
Thr Glu Glu Lys Glu Thr Leu Leu Ser Met Met Ser Pro Met Val Asn
260 265 270
Ala Ile Lys Gln Leu Gln Ala Pro Pro Arg Ser Val Asn Thr Glu Thr
275 280 285
Gly Thr Phe Lys Asp Gly Gly Ser Ala Gly Phe Ser Ala Ala Leu Leu
290 295 300
Pro Leu Leu Gln Ser Leu Gly Glu Lys Glu Leu Ala Ile Glu Gln Ala
305 310 315 320
Lys Val Val Ser Met Leu Leu Gln Ser Asn Ser Asn Asp Tyr Tyr Tyr
325 330 335
Asp Asn Val Leu Ala Leu Phe Gly Met Gly Trp Tyr Gln Gly Lys Tyr
340 345 350
His Phe Gly Ile Asn Gly Glu Leu Gln Pro Ser Trp Val Glu Gln Cys
355 360 365
Gln
<210> 149
<211> 342
<212> PRT
<213> 木醋杆菌
<400> 149
Met Ser Val Met Ala Ala Met Gly Gly Ala Gln Val Leu Ser Ser Thr
1 5 10 15
Gly Ala Phe Ala Asp Thr Ala Pro Asp Ala Val Ala Gln Gln Trp Ala
20 25 30
Ile Phe Arg Ala Lys Tyr Leu Arg Pro Ser Gly Arg Val Val Asp Thr
35 40 45
Gly Asn Gly Gly Glu Ser His Ser Glu Gly Gln Gly Tyr Gly Met Leu
50 55 60
Phe Ala Ala Ser Ala Gly Asp Leu Ala Ser Phe Gln Ser Met Trp Met
65 70 75 80
Trp Ala Arg Thr Asn Leu Gln His Thr Asn Asp Lys Leu Phe Ser Trp
85 90 95
Arg Phe Leu Lys Gly His Gln Pro Pro Val Pro Asp Lys Asn Asn Ala
100 105 110
Thr Asp Gly Asp Leu Leu Ile Ala Leu Ala Leu Gly Arg Ala Gly Lys
115 120 125
Arg Phe Gln Arg Pro Asp Tyr Ile Gln Asp Ala Met Ala Ile Tyr Gly
130 135 140
Asp Val Leu Asn Leu Met Thr Met Lys Ala Gly Pro Tyr Val Val Leu
145 150 155 160
Met Pro Gly Ala Val Gly Phe Thr Lys Lys Asp Ser Val Ile Leu Asn
165 170 175
Leu Ser Tyr Tyr Val Met Pro Ser Leu Leu Gln Ala Phe Asp Leu Thr
180 185 190
Ala Asp Pro Arg Trp Arg Gln Val Met Glu Asp Gly Ile Arg Leu Val
195 200 205
Ser Ala Gly Arg Phe Gly Gln Trp Arg Leu Pro Pro Asp Trp Leu Ala
210 215 220
Val Asn Arg Ala Thr Gly Ala Leu Ser Ile Ala Ser Gly Trp Pro Pro
225 230 235 240
Arg Phe Ser Tyr Asp Ala Ile Arg Val Pro Leu Tyr Phe Tyr Trp Ala
245 250 255
His Met Leu Ala Pro Asn Val Leu Ala Asp Phe Thr Arg Phe Trp Asn
260 265 270
Asn Phe Gly Ala Asn Ala Leu Pro Gly Trp Val Asp Leu Thr Thr Gly
275 280 285
Ala Arg Ser Pro Tyr Asn Ala Pro Pro Gly Tyr Leu Ala Val Ala Glu
290 295 300
Cys Thr Gly Leu Asp Ser Ala Gly Glu Leu Pro Thr Leu Asp His Ala
305 310 315 320
Pro Asp Tyr Tyr Ser Ala Ala Leu Thr Leu Leu Val Tyr Ile Ala Arg
325 330 335
Ala Glu Glu Thr Ile Lys
340
<210> 150
<211> 382
<212> PRT
<213> 紫色色杆菌
<400> 150
Met Ser Ala Val Ser Ile Pro Leu Phe Ala Ala Leu Leu Leu Asp Ala
1 5 10 15
Leu Pro Ala Leu Ala Ala Pro Cys Asp Gly Trp Pro Asp Trp Gln Ala
20 25 30
Phe Leu Lys Asn Tyr Val Ser Gly Asp Gly Arg Val Val Asp Arg Ser
35 40 45
Gln Asp Ala Arg Pro Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe
50 55 60
Phe Ala Leu Ala Ala Asn Asp Arg Pro Ala Phe Asp Arg Leu Leu Ala
65 70 75 80
Trp Thr Arg Asp Asn Leu Ala Asp Gly Asp Leu Gly Ala Arg Leu Pro
85 90 95
Ala Trp Leu Trp Gly Arg Gly Gln Asp Gly Gly Trp Lys Val Leu Asp
100 105 110
Asp Asn Ser Ala Ser Asp Ser Asp Met Trp Leu Ala Tyr Asp Leu Leu
115 120 125
Glu Ala Gly Arg Leu Trp Arg Glu Pro Ser Tyr Ala Ala Gln Gly Arg
130 135 140
Gln Leu Ala Leu Arg Ile Leu Arg Glu Glu Thr Ala Asp Leu Pro Gly
145 150 155 160
Leu Gly Leu Thr Leu Leu Pro Gly Lys Asp Gly Phe Leu Phe Ser Asp
165 170 175
Gly Gly Ala Arg Leu Asn Pro Ser Tyr Leu Pro Pro Gln Leu Leu Ala
180 185 190
Arg Phe Ala Tyr Gly Leu Pro Asp Ser Asp Trp Ala Lys Leu Pro Ser
195 200 205
Gly Ser Glu Arg Val Leu Leu Asp Gly Ala Pro Ala Gly Phe Ala Pro
210 215 220
Asp Trp Leu Arg Leu Gln Pro Gly Lys Gly Leu Gln Pro Asp Ala Asp
225 230 235 240
Ser Lys Ala Lys Gly Gly Tyr Ser Ala Ile Arg Val Tyr Leu Trp Ala
245 250 255
Gly Met Leu Ala Pro Ser Ala Pro Leu Arg Ala Ala Leu Leu Ala Arg
260 265 270
Tyr Lys Pro Met Ala Arg Trp Val Ala Arg Glu Gly Ala Pro Pro Glu
275 280 285
Gln Val Asp Thr Arg Asn Gly Lys Ala Gln Gly Arg Ala Pro Thr Gly
290 295 300
Phe Ser Phe Ala Leu Leu Pro Phe Leu Gln Ala Gln Gly Glu Thr Glu
305 310 315 320
Ala Leu Arg Ala Leu Leu Gln Arg Leu Arg Ala Met Pro Arg Asp Asp
325 330 335
Arg Ala Asp Ala Tyr Tyr Asp Gln Val Leu Arg Leu Phe Gly Leu Gly
340 345 350
Trp Leu Gln Gly Arg Phe Arg Phe Glu Arg Asn Gly Lys Leu Leu Pro
355 360 365
Ala Trp Glu Gly Lys Cys Ser Ala Ser Leu Pro Val Ser Arg
370 375 380
<210> 151
<211> 403
<212> PRT
<213> 丁香假单胞菌
<400> 151
Met Pro Thr Leu Val Ile Asn Leu Arg Arg Ala Val Gly Trp Met Thr
1 5 10 15
Gly Val Leu Ala Ser Ala Thr Leu Thr Pro Ala Leu Ala Ala Ser Thr
20 25 30
Cys Asp Gly Pro Ala Trp Pro Phe Trp Gln Asp Tyr Ala Thr Arg Tyr
35 40 45
Val Gln Ala Asp Gly Arg Met Leu Glu Ser Ser Leu Lys Ala Asn His
50 55 60
Ser Thr Ser Glu Gly Gln Ser Tyr Gly Met Leu Phe Ala Leu Val Ala
65 70 75 80
Asn Asp Arg Ala Arg Phe Asp Thr Leu Trp Thr Trp Thr Ala Glu Asn
85 90 95
Met Thr Gly Ala Asp Pro Arg Thr Arg Leu Pro Gly Trp Leu Trp Gly
100 105 110
Gln Gly Glu Asp Gly Ser Trp Lys Leu Gln Asp Ala Asn Ser Ala Ser
115 120 125
Asp Ala Asp Leu Trp Ile Ile Tyr Ala Leu Leu Glu Ala Ala Arg Ile
130 135 140
Trp Gln His Pro Ala Tyr Arg Asn Asp Ala Leu Ala Leu Leu Lys Thr
145 150 155 160
Val Glu Ala Arg Leu Val Val Asn Leu Pro Gly Leu Gly Lys Met Leu
165 170 175
Leu Pro Gly Pro Glu Gly Phe Val Gln Pro Asp His Leu Trp Arg Leu
180 185 190
Asn Ser Ser Tyr Leu Pro Val Pro Leu Leu Arg Arg Leu Ala Arg Glu
195 200 205
Glu Pro Ala Gly Pro Trp Lys Glu Ile Ala Asp Asn Thr Ala Lys Leu
210 215 220
Ile Asp Ala Ser Ser Pro Lys Gly Phe Ile Ala Asp Trp Val Gly Tyr
225 230 235 240
Arg Gly Thr Ser Pro Lys Thr Gly Leu Phe Val Val Asp Pro Val Ser
245 250 255
Gly Glu Leu Gly Ser Tyr Asp Ala Ile Arg Val Tyr Leu Trp Ala Gly
260 265 270
Met Thr Pro Ala Ser Asp Pro Leu Ala Ala Arg Leu Leu Ala Arg Leu
275 280 285
Asp Gly Met Ala Val Ser Thr Ala Ser Ser Gly Thr Pro Pro Glu Lys
290 295 300
Val Gln Val Met Ser Gly Ala Ser Gln Gly Gln Ala Pro Phe Gly Tyr
305 310 315 320
Ser Ala Ala Leu Leu Pro Tyr Phe Gln Ala Lys Gly Gln Thr Trp Leu
325 330 335
Ala Glu Gln Gln Gln Arg Arg Val Glu Ala Gly Val Ser Thr Ala Leu
340 345 350
Ala Arg Asp Pro Thr Asp Arg Thr Glu Pro Ala Tyr Tyr Asn Leu Met
355 360 365
Leu Ser Leu Phe Ala Leu Gly Trp Val Glu Lys Arg Tyr Gln Phe Arg
370 375 380
Glu Asp Gly Thr Leu Lys Leu Ser Trp Glu Thr Ser Cys Pro Arg Ala
385 390 395 400
Ala Thr Arg
<210> 152
<211> 370
<212> PRT
<213> 恶臭假单胞菌
<400> 152
Met Arg Gln Met Leu Val Gly Leu Val Ala Leu Gly Leu Pro Val Phe
1 5 10 15
Ala Asn Ala Gly Ser Val Cys Pro Trp Pro Ala Trp Glu Arg Phe Lys
20 25 30
Ala Glu Leu Val Ser Val Asp Gly Arg Val Ile Asp Pro Ser Asp Glu
35 40 45
Arg Leu Ile Thr Thr Ser Glu Gly Gln Ser Tyr Ala Leu Phe Phe Ala
50 55 60
Leu Val Gly Asn Asp Arg Gln Thr Phe Ala Gln Leu Leu Arg Trp Thr
65 70 75 80
Ser Asn Asn Leu Ala Glu Gly Asp Leu Ala Arg His Leu Pro Ala Trp
85 90 95
Leu Trp Gly Arg Asp Gly Gln Gln Gln Trp Gln Val Leu Asp Ala Asn
100 105 110
Asn Ala Ser Asp Ala Asp Leu Trp Ile Ala Tyr Ser Leu Leu Glu Ala
115 120 125
Gly Arg Leu Trp Asp Gln Pro Ala Tyr Thr Gln Leu Gly Gln His Leu
130 135 140
Leu Trp Arg Ile Ala Ala Gln Thr Val Arg Lys Leu Pro Gly Leu Gly
145 150 155 160
Val Met Leu Leu Pro Gly Asp Tyr Gly Phe Glu Asp Ala Gln Gly Thr
165 170 175
Arg Leu Asn Pro Ser Tyr Leu Pro Leu Gln Leu Leu Asp Arg Phe Ser
180 185 190
Asp Val Asp Pro Leu Trp Gly Glu Leu Ala Ala Asn Thr Arg Arg Leu
195 200 205
Trp Leu Ala Ser Ser Pro Lys Gly Phe Ala Pro Asp Trp Leu Leu Trp
210 215 220
Thr Pro Ala Gly Lys Pro Ala Ala Asp Thr Lys His Gly Asn Ala Gly
225 230 235 240
Asp Tyr Asp Ala Ile Arg Val Tyr Leu Trp Val Gly Met Leu Ala Glu
245 250 255
Gly Ala Ala Gln Arg Arg Glu Leu Val Ala His Tyr Ala Pro Met Ala
260 265 270
Ala Leu Thr Gln Arg Gln Gly Leu Pro Pro Glu His Leu Asp Ala Arg
275 280 285
Ser Gly Glu Ala Arg Gly His Gly Pro Ala Gly Phe Ser Ala Ala Leu
290 295 300
Leu Pro Leu Leu Ala Ala Ser Pro Glu His Val Ala Gly Leu Ala Ala
305 310 315 320
Gln Arg Gln Arg Leu Arg Glu Gln Pro Val Glu Ala Lys Ala Tyr Tyr
325 330 335
Ser Gln Val Leu Ala Leu Phe Gly Gln Gly Phe Asp Glu Ala Arg Tyr
340 345 350
Arg Phe Asp Pro His Gly Arg Leu Leu Pro Ala Trp Ser Ala Pro Cys
355 360 365
Ser Glu
370
<210> 153
<211> 407
<212> PRT
<213> 环状芽孢杆菌
<400> 153
Met Lys Val Phe Arg Asn Ser Ile Ile Arg Lys Ser Ala Val Leu Phe
1 5 10 15
Cys Ala Val Leu Leu Ile Leu Pro Ala Gly Leu Ser Leu Ala Ala Asn
20 25 30
Lys Pro Phe Pro Gln His Thr Ser Tyr Thr Ser Gly Ser Ile Lys Pro
35 40 45
Asn Asn Val Thr Gln Ser Ala Met Asp Asn Ala Val Lys Ser Lys Trp
50 55 60
Asn Ser Trp Lys Gly Ser Phe Leu Lys Pro Ala Ala Thr Gly Gln Tyr
65 70 75 80
Tyr Val Lys Tyr Asn Ser Ala Gly Glu Thr Val Ser Glu Ala His Gly
85 90 95
Tyr Gly Met Leu Phe Thr Val Leu Met Ala Gly Tyr Asp Ser Asn Ala
100 105 110
Gln Ser Tyr Phe Asp Gly Leu Tyr Arg Tyr Tyr Lys Ala His Pro Ser
115 120 125
Asn Asn Asn Pro Tyr Leu Met Ala Trp Lys Gln Asn Ser Ser Phe Gln
130 135 140
Asn Ile Gly Gly Ala Asn Ser Ala Thr Asp Gly Asp Met Asp Ile Ala
145 150 155 160
Tyr Ala Leu Leu Leu Ala Asp Lys Gln Trp Gly Ser Ser Gly Ser Ile
165 170 175
Asn Tyr Leu Gln Ala Ala Lys Asp Met Ile Asn Ala Ile Met Ser Asn
180 185 190
Asp Val Asn Gln Ser Gln Trp Thr Leu Arg Leu Gly Asp Trp Ala Thr
195 200 205
Ser Gly Ile Phe Asp Thr Ala Thr Arg Pro Ser Asp Phe Met Leu Asn
210 215 220
His Met Lys Ala Phe Arg Ala Ala Thr Gly Asp Ala Arg Trp Glu Asn
225 230 235 240
Val Ile Asn Lys Thr Tyr Thr Ile Ile Asn Ser Ile Tyr Asn Gly Tyr
245 250 255
Ser Ser Asn Thr Gly Leu Leu Pro Asp Phe Val Val Met Ser Gly Gly
260 265 270
Asn Tyr Gln Pro Ala Ala Ala Glu Phe Leu Glu Gly Ala Asn Asp Gly
275 280 285
Lys Tyr Tyr Tyr Asn Ser Ser Arg Thr Pro Trp Arg Ile Thr Thr Asp
290 295 300
Tyr Leu Met Thr Gly Asp Thr Arg Ala Leu Asn Gln Leu Asn Lys Met
305 310 315 320
Asn Thr Phe Ile Lys Ser Ala Thr Ser Ser Asn Pro Ala Asn Val Lys
325 330 335
Ala Gly Tyr Asn Leu Asn Gly Thr Ala Leu Val Thr Tyr Asn Ser Gly
340 345 350
Ala Phe Tyr Ala Pro Phe Gly Val Ser Ala Met Thr Ser Ser Ser His
355 360 365
Gln Ser Trp Leu Asn Ser Val Trp Ser Tyr Thr Ala Asn Ala Ser Ala
370 375 380
Glu Gly Tyr Tyr Glu Glu Ser Ile Lys Leu Phe Ser Met Ile Val Met
385 390 395 400
Ser Gly Asn Trp Trp Thr Tyr
405
<210> 154
<211> 797
<212> PRT
<213> Paenibacillus fukuinensis
<400> 154
Met Phe Thr Ser Ser Leu Ser Gly Ser Pro Arg Leu Lys Ser Ala Phe
1 5 10 15
Leu Leu Leu Leu Cys Leu Ala Met Val Val Ser Ile Gly Phe Val Pro
20 25 30
Lys Gly Asn Glu Gly Arg Val His Ala Ala Gly Glu Met Met Pro Phe
35 40 45
Pro Gln Gln Val Ser Tyr Ser Gly Ile Ile Lys Pro Asn His Val Thr
50 55 60
Gln Ala Ala Met Asn Thr Ala Val Ala Ala Tyr Tyr Asp Tyr Trp Lys
65 70 75 80
Gly Lys Tyr Leu Lys Asn Asn Leu Ser Ser Leu Pro Gly Gly Tyr Tyr
85 90 95
Val Lys Gly Glu Ile Thr Gly Ser Pro Glu Gly Phe Val Pro Leu Gly
100 105 110
Thr Ser Glu Gly Gln Gly Tyr Gly Met Ile Ile Thr Ala Leu Met Ala
115 120 125
Gly His Asp Pro Asn Ala Gln Thr Ile Phe Asn Gly Leu Phe Lys Thr
130 135 140
Ala Arg Ala Tyr Lys Ser Ser Gly Asn Pro Asn Leu Met Gly Trp Val
145 150 155 160
Val Ala Asp His Ile Asn Ala Gln Gly His Phe Gly Ser Ala Thr Asp
165 170 175
Gly Asp Leu Asp Ile Ala Tyr Ser Leu Leu Leu Ala His Lys Gln Trp
180 185 190
Gly Ser Asn Gly Thr Val Asn Tyr Leu Ala Glu Ala Gln Asn Met Ile
195 200 205
Thr Asn Gly Ile Lys Ala Ser Tyr Val Thr Thr Asn Asn Arg Leu Asn
210 215 220
Leu Gly Asp Trp Asp Ser Lys Ser Ser Leu Ala Thr Arg Pro Ser Asp
225 230 235 240
Trp Met Leu Ser His Leu Arg Ala Phe Tyr Glu Phe Thr Gly Asp Gln
245 250 255
Thr Trp Ile Asn Val Ile Asn Asn Leu Tyr Asn Val Tyr Thr Gln Val
260 265 270
Ser Asn Asn Tyr Ala Ser Ser Thr Gly Leu Ile Ser Asp Phe Val Val
275 280 285
Asn Asn Pro Pro Gln Pro Ala Pro Glu Trp Tyr Leu Asn Glu Phe Gln
290 295 300
Gln Thr Asn Ala Tyr Tyr Tyr Asn Ala Ala Arg Val Pro Leu Arg Ile
305 310 315 320
Val Met Asp Tyr Ala Met Tyr Gly Asp Thr Arg Gly Lys Thr Ile Ala
325 330 335
Asp Lys Ile Ala Val Trp Ile Lys Gly Lys Ala Ser Asn Ser Pro Ala
340 345 350
Asn Ile Arg Asp Gly Tyr Gln Leu Asn Gly Thr Thr Ile Gly Gly Tyr
355 360 365
Ala Thr Ala Val Phe Val Ser Pro Phe Ile Ala Ala Ser Thr Thr Ser
370 375 380
Thr Ser His Gln Ala Trp Val Asn Ala Gly Trp Asp Trp Met Lys Asn
385 390 395 400
Lys Gln Glu Asn Tyr Phe Ser Asp Ser Tyr Asn Leu Met Thr Met Leu
405 410 415
Phe Ile Thr Gly Asn Trp Trp Lys Pro Thr Ala Ala Ser Ser Asp Thr
420 425 430
Gln Ala Pro Thr Val Pro Gly Ser Leu Thr Ala Ala Ala Thr Ser Ser
435 440 445
Ser Ser Ile Asn Leu Thr Trp Thr Ala Ser Thr Asp Asn Val Gly Val
450 455 460
Thr Gly Tyr Arg Ile Tyr Arg Gly Gly Thr Gln Val Gly Thr Ala Thr
465 470 475 480
Gly Leu Ser Tyr Ala Asp Ser Gly Leu Ser Ala Asn Thr Ser Tyr Ser
485 490 495
Tyr Thr Val Arg Ala Val Asp Ala Ala Gly Asn Val Ser Gly Asn Ser
500 505 510
Asn Thr Ala Ser Ala Thr Thr Leu Ser Gly Thr Thr Pro Pro Thr Gly
515 520 525
Thr Asn Leu Ala Leu Asn Lys Thr Ala Thr Ala Ser Ser Ile Glu Gly
530 535 540
Ala Gly Phe Glu Ala Ser Arg Ala Phe Asp Gly Ser Ser Thr Thr Arg
545 550 555 560
Trp Ala Ser Ala Glu Gly Val Asp Pro Gln Trp Ile Tyr Val Asn Leu
565 570 575
Gly Ser Ser Gln Thr Val Asn Arg Val Lys Leu Asn Trp Glu Ala Ala
580 585 590
Tyr Ala Ser Ser Tyr Thr Ile Gln Val Ser Asn Asp Ser Gly Thr Pro
595 600 605
Thr Asn Trp Thr Thr Val Tyr Thr Thr Thr Thr Gly Asp Gly Gly Ile
610 615 620
Asp Asp Ile Thr Phe Thr Ala Arg Thr Ala Lys Tyr Val Arg Val His
625 630 635 640
Gly Thr Val Arg Gly Thr Pro Tyr Gly Tyr Ser Leu Trp Glu Phe Glu
645 650 655
Val Tyr Gly Gly Ser Thr Ala Pro Ser Asn Leu Ala Leu Asn Lys Ala
660 665 670
Thr Ala Thr Ser Ser Ile Glu Thr Ala Gly His Glu Gly Asp Lys Ala
675 680 685
Val Asp Gly Asn Ala Ala Thr Arg Trp Ala Ser Ala Tyr Gly Ala Ser
690 695 700
Pro Gln Trp Ile Tyr Ile Asn Leu Gly Ser Thr Gln Ser Ile Ser Arg
705 710 715 720
Val Lys Leu Asn Trp Glu Asp Ala Tyr Ala Thr Ala Tyr Ser Ile Gln
725 730 735
Val Ser Asn Asp Ser Gly Ser Thr Pro Thr Asn Trp Thr Thr Val Tyr
740 745 750
Ser Thr Thr Thr Gly Asp Gly Ala Ile Asp Asp Ile Thr Phe Ala Ala
755 760 765
Thr Asn Ala Lys Phe Val Arg Val Tyr Ala Thr Thr Arg Ala Thr Ala
770 775 780
Tyr Gly Tyr Ser Leu Trp Glu Phe Glu Val Tyr Gly Ala
785 790 795
<210> 155
<211> 4
<212> PRT
<213> 人工序列
<220>
<223> GH木聚糖酶保守基序2
<220>
<221> NON_CONS
<222> (1)..(1)
<223> 保守基序位置1的氨基酸为天冬酰胺(N)或天冬氨酸(D)
<220>
<221> NON_CONS
<222> (2)..(2)
<223> 保守基序位置2的氨基酸为丙氨酸(A)或丝氨酸(S)
<220>
<221> NON_CONS
<222> (3)..(3)
<223> 保守基序位置3的氨基酸为任意氨基酸
<220>
<221> NON_CONS
<222> (4)..(4)
<223> 保守基序位置4的氨基酸为精氨酸(R), 亮氨酸(L), 脯氨酸(P)或谷氨酰胺(Q)
<400> 155
Xaa Xaa Xaa Xaa
1
<210> 156
<211> 42
<212> DNA
<213> 人工序列
<220>
<223> 引物LBei411f
<400> 156
gcttctacaa cagacgctga caaccttggc aacggctatt ac 42
<210> 157
<211> 42
<212> DNA
<213> 人工序列
<220>
<223> 引物LBei411r
<400> 157
gtaatagccg ttgccaaggt tgtcagcgtc tgttgtagaa gc 42
<210> 158
<211> 1299
<212> DNA
<213> Pseudoalteromonas haloplanktis
<220>
<221> CDS
<222> (1)..(1296)
<220>
<221> 信号肽
<222> (1)..(81)
<220>
<221> 成熟肽
<222> (82)..(1296)
<400> 158
atg aag aaa ccg ttg ggg aaa att gtc gca agc acc gca cta ctc att 48
Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile
-25 -20 -15
tct gtt gct ttt agt tca tcg atc gca tcg gct gca ttt aat aat aac 96
Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Phe Asn Asn Asn
-10 -5 -1 1 5
cca tcg agt gta ggc gcc gac agt tca gga gca tac cgt aac ctc gca 144
Pro Ser Ser Val Gly Ala Asp Ser Ser Gly Ala Tyr Arg Asn Leu Ala
10 15 20
caa gaa atg ggt aaa aca aat ata cag caa aaa gtg aat agt act ttt 192
Gln Glu Met Gly Lys Thr Asn Ile Gln Gln Lys Val Asn Ser Thr Phe
25 30 35
gac aat atg ttt ggc tat aac aac aca caa caa ctt tac tac ccg tac 240
Asp Asn Met Phe Gly Tyr Asn Asn Thr Gln Gln Leu Tyr Tyr Pro Tyr
40 45 50
acc gaa aac ggt gtt tat aaa gca cac tac ata aaa gca att aac cca 288
Thr Glu Asn Gly Val Tyr Lys Ala His Tyr Ile Lys Ala Ile Asn Pro
55 60 65
gac gaa ggc gac gat ata aga aca gaa ggg caa tcg tgg gga atg acc 336
Asp Glu Gly Asp Asp Ile Arg Thr Glu Gly Gln Ser Trp Gly Met Thr
70 75 80 85
gcc gct gtc atg ctt aat aaa caa gaa gaa ttt gat aac cta tgg cgc 384
Ala Ala Val Met Leu Asn Lys Gln Glu Glu Phe Asp Asn Leu Trp Arg
90 95 100
ttt gca aaa gcg tat caa aaa aat cca gac aac cac cct gat gct aaa 432
Phe Ala Lys Ala Tyr Gln Lys Asn Pro Asp Asn His Pro Asp Ala Lys
105 110 115
aaa caa ggc gtt tac gcg tgg aaa cta aag ctt aat caa aac ggc ttt 480
Lys Gln Gly Val Tyr Ala Trp Lys Leu Lys Leu Asn Gln Asn Gly Phe
120 125 130
gtt tat aaa gta gat gaa ggc ccc gct ccc gat ggc gaa gag tac ttt 528
Val Tyr Lys Val Asp Glu Gly Pro Ala Pro Asp Gly Glu Glu Tyr Phe
135 140 145
gct ttt gca cta ctt aat gcc tcc gct cgt tgg ggc aat tcg ggt gag 576
Ala Phe Ala Leu Leu Asn Ala Ser Ala Arg Trp Gly Asn Ser Gly Glu
150 155 160 165
ttt aac tac tac aac gat gcc att acc atg tta aac acg att aaa agt 624
Phe Asn Tyr Tyr Asn Asp Ala Ile Thr Met Leu Asn Thr Ile Lys Ser
170 175 180
aag ttg atg gaa aac caa ata atc cgc ttt tca cct tac att gat aac 672
Lys Leu Met Glu Asn Gln Ile Ile Arg Phe Ser Pro Tyr Ile Asp Asn
185 190 195
cta aca gac cct tct tat cac ata cct gcg ttt tat gac tac ttt gca 720
Leu Thr Asp Pro Ser Tyr His Ile Pro Ala Phe Tyr Asp Tyr Phe Ala
200 205 210
aat aac gta act aac caa gca gac aaa act tac tgg cga caa gtc gcc 768
Asn Asn Val Thr Asn Gln Ala Asp Lys Thr Tyr Trp Arg Gln Val Ala
215 220 225
aca aaa agt aga acc tta ctt aaa aac cat ttt aca aaa gta agt ggt 816
Thr Lys Ser Arg Thr Leu Leu Lys Asn His Phe Thr Lys Val Ser Gly
230 235 240 245
agc ccg cat tgg aac tta cct aca ttt tta tca cgc tta gat ggc agc 864
Ser Pro His Trp Asn Leu Pro Thr Phe Leu Ser Arg Leu Asp Gly Ser
250 255 260
cct gtt att ggc tac att ttt aac gac caa gca aac cca ggt caa tgg 912
Pro Val Ile Gly Tyr Ile Phe Asn Asp Gln Ala Asn Pro Gly Gln Trp
265 270 275
tat gaa ttt gat gca tgg cgc gta att atg aat gtg ggt tta gac gcg 960
Tyr Glu Phe Asp Ala Trp Arg Val Ile Met Asn Val Gly Leu Asp Ala
280 285 290
cat tta atg ggt gct caa gcg tgg cat aaa agc gca gta aat aaa gca 1008
His Leu Met Gly Ala Gln Ala Trp His Lys Ser Ala Val Asn Lys Ala
295 300 305
ctg ggc ttt tta agt tat gca aaa act aac aac agt aat aac tgt tac 1056
Leu Gly Phe Leu Ser Tyr Ala Lys Thr Asn Asn Ser Asn Asn Cys Tyr
310 315 320 325
gag caa gtg tat tcg tac ggt ggg gcg caa aac aga ggc tgt gcg ggc 1104
Glu Gln Val Tyr Ser Tyr Gly Gly Ala Gln Asn Arg Gly Cys Ala Gly
330 335 340
gaa ggt caa aaa gcg gct aat gca gta gcg cta ctg gct tca aca aat 1152
Glu Gly Gln Lys Ala Ala Asn Ala Val Ala Leu Leu Ala Ser Thr Asn
345 350 355
gct ggg caa gca act gag ttt ttt aac gaa ttt tgg tct tta tcg caa 1200
Ala Gly Gln Ala Thr Glu Phe Phe Asn Glu Phe Trp Ser Leu Ser Gln
360 365 370
cca aca ggt gat tat cgt tac tac aat ggt tcg ctg tat atg tta gct 1248
Pro Thr Gly Asp Tyr Arg Tyr Tyr Asn Gly Ser Leu Tyr Met Leu Ala
375 380 385
atg ctg cat gta tcg ggt aat ttt aag ttt tat aac aac acg ttt aat 1296
Met Leu His Val Ser Gly Asn Phe Lys Phe Tyr Asn Asn Thr Phe Asn
390 395 400 405
taa 1299
<210> 159
<211> 432
<212> PRT
<213> Pseudoalteromonas haloplanktis
<400> 159
Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile
-25 -20 -15
Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Phe Asn Asn Asn
-10 -5 -1 1 5
Pro Ser Ser Val Gly Ala Asp Ser Ser Gly Ala Tyr Arg Asn Leu Ala
10 15 20
Gln Glu Met Gly Lys Thr Asn Ile Gln Gln Lys Val Asn Ser Thr Phe
25 30 35
Asp Asn Met Phe Gly Tyr Asn Asn Thr Gln Gln Leu Tyr Tyr Pro Tyr
40 45 50
Thr Glu Asn Gly Val Tyr Lys Ala His Tyr Ile Lys Ala Ile Asn Pro
55 60 65
Asp Glu Gly Asp Asp Ile Arg Thr Glu Gly Gln Ser Trp Gly Met Thr
70 75 80 85
Ala Ala Val Met Leu Asn Lys Gln Glu Glu Phe Asp Asn Leu Trp Arg
90 95 100
Phe Ala Lys Ala Tyr Gln Lys Asn Pro Asp Asn His Pro Asp Ala Lys
105 110 115
Lys Gln Gly Val Tyr Ala Trp Lys Leu Lys Leu Asn Gln Asn Gly Phe
120 125 130
Val Tyr Lys Val Asp Glu Gly Pro Ala Pro Asp Gly Glu Glu Tyr Phe
135 140 145
Ala Phe Ala Leu Leu Asn Ala Ser Ala Arg Trp Gly Asn Ser Gly Glu
150 155 160 165
Phe Asn Tyr Tyr Asn Asp Ala Ile Thr Met Leu Asn Thr Ile Lys Ser
170 175 180
Lys Leu Met Glu Asn Gln Ile Ile Arg Phe Ser Pro Tyr Ile Asp Asn
185 190 195
Leu Thr Asp Pro Ser Tyr His Ile Pro Ala Phe Tyr Asp Tyr Phe Ala
200 205 210
Asn Asn Val Thr Asn Gln Ala Asp Lys Thr Tyr Trp Arg Gln Val Ala
215 220 225
Thr Lys Ser Arg Thr Leu Leu Lys Asn His Phe Thr Lys Val Ser Gly
230 235 240 245
Ser Pro His Trp Asn Leu Pro Thr Phe Leu Ser Arg Leu Asp Gly Ser
250 255 260
Pro Val Ile Gly Tyr Ile Phe Asn Asp Gln Ala Asn Pro Gly Gln Trp
265 270 275
Tyr Glu Phe Asp Ala Trp Arg Val Ile Met Asn Val Gly Leu Asp Ala
280 285 290
His Leu Met Gly Ala Gln Ala Trp His Lys Ser Ala Val Asn Lys Ala
295 300 305
Leu Gly Phe Leu Ser Tyr Ala Lys Thr Asn Asn Ser Asn Asn Cys Tyr
310 315 320 325
Glu Gln Val Tyr Ser Tyr Gly Gly Ala Gln Asn Arg Gly Cys Ala Gly
330 335 340
Glu Gly Gln Lys Ala Ala Asn Ala Val Ala Leu Leu Ala Ser Thr Asn
345 350 355
Ala Gly Gln Ala Thr Glu Phe Phe Asn Glu Phe Trp Ser Leu Ser Gln
360 365 370
Pro Thr Gly Asp Tyr Arg Tyr Tyr Asn Gly Ser Leu Tyr Met Leu Ala
375 380 385
Met Leu His Val Ser Gly Asn Phe Lys Phe Tyr Asn Asn Thr Phe Asn
390 395 400 405